Spg025336 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025336 vs. NCBI nr
Match: KAA0040037.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 330.9 bits (847), Expect = 1.7e-86
Identity = 180/541 (33.27%), Postives = 273/541 (50.46%), Query Frame = 0

Query: 4   GSFNLPSIV-EDDILIFLQDDDNVLDNMLYIIKSFEQASGLKINLSKSTVSGINVTDQRV 63
           G+ NL  ++  DDIL+F++DD++ + N+  II  F+ ASGL INL+KST+S INV   R 
Sbjct: 3   GNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLASGLSINLNKSTISPINVDAART 62

Query: 64  SMIASKWGCSFQALPISYLGMPLGGKPRSSSFWDPLVEKIHRKIDNWRFASLSKGGRLTL 123
             IAS+WG S + LPI+YLG+PLGGK  + +FW  + EKI++K+ +W+++ LSKGG++TL
Sbjct: 63  EQIASQWGISTKFLPINYLGVPLGGKQITKTFWKNVEEKINKKLASWKYSMLSKGGKITL 122

Query: 124 LQSVLNFMPLYLSAVLKVPISVCKRIEKIFRKFLWDGIDKSSSSHLIRWDMVTMPKSEGG 183
           ++S L  +P Y  ++ K P+S CK IEK +R FLW    ++   HL+ W  +T PK  GG
Sbjct: 123 IKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNPPETHKLHLVNWAKITSPKERGG 182

Query: 184 LGLPKFKQMNEALLMKWIWRFYNEDDSLWCKLINYKYKSLTPGSIPSISKYASSRSPWMA 243
           LG+ + K  N ALL KW+WR+ +ED  LW K+IN KY+SL+ G IP +  ++SSRSPW +
Sbjct: 183 LGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSSSRSPWFS 242

Query: 244 ISNLQDIFWANSRWELRDGRRILFWFDRWTGSAPLRFVGNRFFSLAADKNICVEKAWMNT 303
           I    + F  +  W++++GR   FW   W  ++PL F   R F+L+ +K+  +   W NT
Sbjct: 243 ICKGLEWFQRHVSWKIKNGRSFSFWHSHWHQNSPLSFHYPRLFALSTNKDSSIRDMWNNT 302

Query: 304 NL-WNFFPRRPLLDRENSVWADFTASLPKLDLSNG------------------------- 363
            + W+  PRR L + E  +WA+   SL      NG                         
Sbjct: 303 LMDWDLNPRRQLREWEYPLWAELKNSLNASFCENGKDSPTWTLNSNGLYTVASVKKALQQ 362

Query: 364 ------------------------------------------------------------ 423
                                                                       
Sbjct: 363 PDQSLLDLQSQNTFKNLWKTSIPKKCIFFIWTLLYDSVNTAEQLMKRLPNLCSRPSWCVM 422

Query: 424 ------------------------ISSAIPLHAGC-----LCEEFLAKKALSQRDTIILN 429
                                   ISS +  +  C     LC    + K  ++++ I+ N
Sbjct: 423 CKRNDEDRIHLFILCPIAKSIWRLISSHLNSNVNCLSPKDLCITMCSWKQKTKKNVILYN 482

BLAST of Spg025336 vs. NCBI nr
Match: XP_016902461.1 (PREDICTED: LINE-1 retrotransposable element ORF2 protein [Cucumis melo])

HSP 1 Score: 327.4 bits (838), Expect = 1.8e-85
Identity = 178/541 (32.90%), Postives = 273/541 (50.46%), Query Frame = 0

Query: 4    GSFNLPSIV-EDDILIFLQDDDNVLDNMLYIIKSFEQASGLKINLSKSTVSGINVTDQRV 63
            G+ NL  ++  DDIL+F++DD++ + N+  II  F+ ASGL INL+KST+S INV   R 
Sbjct: 516  GNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLASGLSINLNKSTISPINVDASRT 575

Query: 64   SMIASKWGCSFQALPISYLGMPLGGKPRSSSFWDPLVEKIHRKIDNWRFASLSKGGRLTL 123
              IAS+WG S + LPI+YLG+PLGGK  + +FW  + EKI++K+ +W+++ LSKGG++TL
Sbjct: 576  EQIASQWGISTKFLPINYLGVPLGGKQITKTFWKNVEEKINKKLASWKYSMLSKGGKITL 635

Query: 124  LQSVLNFMPLYLSAVLKVPISVCKRIEKIFRKFLWDGIDKSSSSHLIRWDMVTMPKSEGG 183
            ++S L  +P Y  ++ KVP+S CK IEK +R FLW    ++   HL+ W  +T  K +GG
Sbjct: 636  IKSSLASLPTYQLSIFKVPVSTCKNIEKTWRNFLWKNPPETHKLHLVNWAKITSSKEKGG 695

Query: 184  LGLPKFKQMNEALLMKWIWRFYNEDDSLWCKLINYKYKSLTPGSIPSISKYASSRSPWMA 243
            LG+ + K  N ALL KW+WR+ +ED  LW K+IN KY+SL+ G IP +  ++SSRSPW +
Sbjct: 696  LGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSSSRSPWFS 755

Query: 244  ISNLQDIFWANSRWELRDGRRILFWFDRWTGSAPLRFVGNRFFSLAADKNICVEKAWMNT 303
            I    + F  +  W++++GR   FW   W  ++PL     R ++L+ +K   +   W NT
Sbjct: 756  ICKGLEWFQRHVSWKIKNGRSFSFWHSHWHQNSPLSSHYPRLYALSTNKESSIRDMWNNT 815

Query: 304  NL-WNFFPRRPLLDRENSVWADFTASLPKLDLSNG------------------------- 363
             + W+  PRR L + E+ +WA+   SL      NG                         
Sbjct: 816  LMDWDLNPRRQLREWEHPLWAELKNSLNASFCENGNDSPMWTLNSNGLYTVASVKKTLQQ 875

Query: 364  ------------------------------------------------------------ 423
                                                                        
Sbjct: 876  PEQNLLDFQSQNTFKNLWKTSIPKKCIFFIWTLLYDSVNTAEQLMKRLPNLCSRPSWCVM 935

Query: 424  ------------------------ISSAIPLHAGC-----LCEEFLAKKALSQRDTIILN 429
                                    ISS +  +  C     LC    + K  ++++ I+ N
Sbjct: 936  CKRNDEDRIHLFILCPIAKSIWRSISSHLSSNVNCLSPKELCITMCSWKQKTKKNVILFN 995

BLAST of Spg025336 vs. NCBI nr
Match: KAA0041397.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 325.9 bits (834), Expect = 5.4e-85
Identity = 185/541 (34.20%), Postives = 258/541 (47.69%), Query Frame = 0

Query: 5    SFNLPSIV-EDDILIFLQDDDNVLDNMLYIIKSFEQASGLKINLSKSTVSGINVTDQRVS 64
            + NL  I+  DDILIF++D ++ + N+  I+  FE ASGL INLSKST+  INV   R +
Sbjct: 497  NLNLTHILFADDILIFVEDKEDYVSNLKMILHLFESASGLNINLSKSTIFPINVPTDRAN 556

Query: 65   MIASKWGCSFQALPISYLGMPLGGKPRSSSFWDPLVEKIHRKIDNWRFASLSKGGRLTLL 124
             I   WG S   LP +YLGMPLGGKP SS+FWD +++KI +K+ +W+++ LSKGGR+TL+
Sbjct: 557  SIVDSWGISKGQLPTTYLGMPLGGKPSSSNFWDNILQKIQKKLSSWKYSQLSKGGRITLI 616

Query: 125  QSVLNFMPLYLSAVLKVPISVCKRIEKIFRKFLWDGIDKSSSSHLIRWDMVTMPKSEGGL 184
             S L  +P+Y  +V KVP  + ++IE  +R FLW+G     +  LIRW+ V  PK +GGL
Sbjct: 617  NSTLESLPIYQLSVFKVPKGIAQKIEAYWRNFLWNGTSNGHNISLIRWNQVVSPKEKGGL 676

Query: 185  GLPKFKQMNEALLMKWIWRFYNEDDSLWCKLINYKYKSLTPGSIPSISKYASSRSPWMAI 244
            G+      N ALL KW+W+F  E + LW +LI  KY     G  PS  KY+S+ SPW A+
Sbjct: 677  GIHSVHSTNFALLCKWLWKFLTEKEPLWKRLIISKYDQEKMGRFPSRGKYSSNNSPWKAV 736

Query: 245  SNLQDIFWANSRWELRDGRRILFWFDRWTGSAPLRFVGNRFFSLAADKNICVEKAWMNTN 304
            +N    F+ N  W++ DG  I FW D W G++PL  V  R F+L+ +K   V+  W N +
Sbjct: 737  TNCISWFYKNIGWKVNDGEDISFWLDNWNGNSPLSLVVPRLFALSTNKKGSVKDLW-NPS 796

Query: 305  L--WNFFPRRPLLDRENSVWADFTASLP--------------------------KLDLSN 364
            L  WN    RPL D E ++W +  ASLP                          K DLS 
Sbjct: 797  LKDWNIHVNRPLRDHEKNLWHNIKASLPTPLPDRGPSKPLWKLNSNNIFDTASIKKDLSE 856

Query: 365  GISSAIPLHA------------------------GC------------------------ 424
              +S    H                         GC                        
Sbjct: 857  ASASPTNFHPSLYKTLWKVDFPKKCKFFIWTLIHGCINTADRLQKRLPNWTLSPNWCYMC 916

Query: 425  ---------------------------------------LCEEFLAKKALSQRDTIILNI 430
                                                   L +   +    +Q+  I  N 
Sbjct: 917  NKSQEDINHLFIHCPYSQQLWSKAQALLKWNSTPNDVKSLAQNICSLNIKTQKGLITFNT 976

BLAST of Spg025336 vs. NCBI nr
Match: KAA0039770.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK24727.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 325.9 bits (834), Expect = 5.4e-85
Identity = 177/541 (32.72%), Postives = 272/541 (50.28%), Query Frame = 0

Query: 4    GSFNLPSIV-EDDILIFLQDDDNVLDNMLYIIKSFEQASGLKINLSKSTVSGINVTDQRV 63
            G+ NL  ++  DDIL+F++DD++ + N+  II  F+ ASGL INL+KST+S INV   R 
Sbjct: 516  GNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLASGLSINLNKSTISPINVDASRT 575

Query: 64   SMIASKWGCSFQALPISYLGMPLGGKPRSSSFWDPLVEKIHRKIDNWRFASLSKGGRLTL 123
              IAS+WG S + LPI+YLG+PLGGK  + +FW  + EKI++K+ +W+++ LSKGG++TL
Sbjct: 576  EQIASQWGISTKFLPINYLGVPLGGKQITKTFWKNVEEKINKKLASWKYSMLSKGGKITL 635

Query: 124  LQSVLNFMPLYLSAVLKVPISVCKRIEKIFRKFLWDGIDKSSSSHLIRWDMVTMPKSEGG 183
            ++S L  +P Y  ++ K P+S CK IEK +R FLW    ++   HL+ W  +T  K +GG
Sbjct: 636  IKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNPPETHKLHLVNWAKITSSKEKGG 695

Query: 184  LGLPKFKQMNEALLMKWIWRFYNEDDSLWCKLINYKYKSLTPGSIPSISKYASSRSPWMA 243
            LG+ + K  N ALL KW+WR+ +ED  LW K+IN KY+SL+ G IP +  ++SSRSPW +
Sbjct: 696  LGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSSSRSPWFS 755

Query: 244  ISNLQDIFWANSRWELRDGRRILFWFDRWTGSAPLRFVGNRFFSLAADKNICVEKAWMNT 303
            I    + F  +  W++++GR   FW   W  ++PL     R ++L+ +K   +   W NT
Sbjct: 756  ICKGLEWFQRHVSWKIKNGRSFSFWHSHWHQNSPLSSHYPRLYALSTNKESSIRDMWNNT 815

Query: 304  NL-WNFFPRRPLLDRENSVWADFTASLPKLDLSNG------------------------- 363
             + W+  PRR L + E+ +WA+   SL      NG                         
Sbjct: 816  LMDWDLNPRRQLREWEHPLWAELKNSLNASFCENGNDSPMWTLNSNGLYTVASVKKTLQQ 875

Query: 364  ------------------------------------------------------------ 423
                                                                        
Sbjct: 876  PEQNLLDFQSQNTFKNLWKTSIPKKCIFFIWTLLYDSVNTAEQLMKRLPNLCSRPSWCVM 935

Query: 424  ------------------------ISSAIPLHAGC-----LCEEFLAKKALSQRDTIILN 429
                                    ISS +  +  C     LC    + K  ++++ I+ N
Sbjct: 936  CKRNDEDRIHLFILCPIAKSIWRSISSHLSSNVNCLSPKELCITMCSWKQKTKKNVILFN 995

BLAST of Spg025336 vs. NCBI nr
Match: TYK06777.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 323.9 bits (829), Expect = 2.0e-84
Identity = 178/541 (32.90%), Postives = 273/541 (50.46%), Query Frame = 0

Query: 4    GSFNLPSIV-EDDILIFLQDDDNVLDNMLYIIKSFEQASGLKINLSKSTVSGINVTDQRV 63
            G+ NL  ++  DDIL+F++DD++ + N+  II  F+ ASGL INL+KST+S INV   R 
Sbjct: 579  GNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLASGLSINLNKSTISPINVDASRT 638

Query: 64   SMIASKWGCSFQALPISYLGMPLGGKPRSSSFWDPLVEKIHRKIDNWRFASLSKGGRLTL 123
              IAS+WG S + LPI+YLG+PLGGK  + +FW  + EKI++K+ +W+++ LSKGG++TL
Sbjct: 639  EQIASQWGISTKFLPINYLGVPLGGKQTTKAFWKNVEEKINKKLASWKYSMLSKGGKITL 698

Query: 124  LQSVLNFMPLYLSAVLKVPISVCKRIEKIFRKFLWDGIDKSSSSHLIRWDMVTMPKSEGG 183
            ++S L  +P Y  ++ K P+S CK IEK +R FLW    ++   HL+ W  +T  K +GG
Sbjct: 699  IKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNPPETHKLHLVNWAKITSSKEKGG 758

Query: 184  LGLPKFKQMNEALLMKWIWRFYNEDDSLWCKLINYKYKSLTPGSIPSISKYASSRSPWMA 243
            LG+ + K  N ALL KW+WR+ +ED  LW K+IN KY+SL+ G IP +  ++SSRSPW +
Sbjct: 759  LGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSSSRSPWFS 818

Query: 244  ISNLQDIFWANSRWELRDGRRILFWFDRWTGSAPLRFVGNRFFSLAADKNICVEKAWMNT 303
            I    + F  +  W++++GR   FW   W  ++PL     R ++L+ +K   +   W NT
Sbjct: 819  ICKGLEWFQRHVSWKIKNGRSFSFWHGHWHQNSPLSSHYPRLYALSTNKESSIRDMWNNT 878

Query: 304  NL-WNFFPRRPLLDRENSVWADFTAS---------------------------------- 363
             + W+  PRR L + E  +WA+   S                                  
Sbjct: 879  LMDWDLNPRRQLREWELPLWAELKNSINASFCENGKDSPMWALNSNGLYTVASVKKALQQ 938

Query: 364  -----------------------------------------------LPKL--------- 423
                                                           LPKL         
Sbjct: 939  PEQSLLDLQSQNTFKNLWKTSIPKKCIFFIWTLLYDSVNTAEQLMKRLPKLCSRPSWCVM 998

Query: 424  -------------------DLSNGISSAIPLHAGC-----LCEEFLAKKALSQRDTIILN 429
                                +   ISS +  +  C     LC    + K  ++++ I+ N
Sbjct: 999  CKRNDEDRIHLFILCPIAKSIWRTISSHLNSNVNCLSPKELCITMCSWKQKTKKNVILFN 1058

BLAST of Spg025336 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.5e-29
Identity = 71/226 (31.42%), Postives = 114/226 (50.44%), Query Frame = 0

Query: 83  MPLGGKPRSSSFWDPLVEKIHRKIDNWRFASLSKGGRLTLLQSVLNFMPLYLSAVLKVPI 142
           MP+  K  +   +  ++E++  ++  WR  +LS  GRLTL ++VL+ MP++  + + +P 
Sbjct: 1   MPVLQKRINKDTFGEILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQ 60

Query: 143 SVCKRIEKIFRKFLWDGIDKSSSSHLIRWDMVTMPKSEGGLGLPKFKQMNEALLMKWIWR 202
           S+  R++++ R FLW    +    HL++W  V  PK EGGLG+   K MN AL+ K  WR
Sbjct: 61  SILNRLDQLSRTFLWGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWR 120

Query: 203 FYNEDDSLWCKLINYKYKSLTPGSIPSISKYASSRSPWMAIS-NLQDIFWANSRWELRDG 262
              E +SLW  ++  KY          +    S  S W +I+  L+D+      W   DG
Sbjct: 121 LLQEKNSLWTLVLQKKYHVGEIRDSRWLIPKGSWSSTWRSIAIGLRDVVSHGVGWIPGDG 180

Query: 263 RRILFWFDRWTGSAPLRFVGNRFFSLAADKNICVEKAWMNTNLWNF 308
           ++I FW DRW    PL  + N       D  +  +  W+    W+F
Sbjct: 181 QQIRFWTDRWVSGKPLLELDNGERPTDCD-TVVAKDLWIPGRGWDF 225

BLAST of Spg025336 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 5.0e-09
Identity = 49/219 (22.37%), Postives = 101/219 (46.12%), Query Frame = 0

Query: 10  SIVEDDILIFLQDDDNVLDNMLYIIKSFEQASGLKINLSKSTVSGINVTDQRVSMIASKW 69
           S+  DD++++L++  +    +L +IK +   SG KIN  KS        +Q    +    
Sbjct: 697 SLFADDMIVYLENTRDSTTKLLEVIKEYSNVSGYKINTHKSVAFIYTNNNQAEKTVKDSI 756

Query: 70  GCSFQALPISYLGMPL--GGKPRSSSFWDPLVEKIHRKIDNWRFASLSKGGRLTLLQ-SV 129
             +     + YLG+ L    K      ++ L ++I   ++ W+    S  GR+ +++ S+
Sbjct: 757 PFTVVPKKMKYLGVYLTKDVKDLYKENYETLRKEIAEDVNKWKNIPCSWLGRINIVKMSI 816

Query: 130 LNFMPLYLSAV-LKVPISVCKRIEKIFRKFLWDGIDKSSSSHLIRWDMVTMPKSEGGLGL 189
           L       +A+ +K P+S  K +EKI   F+W     +     I   +++     GG+ L
Sbjct: 817 LPKAIYNFNAIPIKAPLSYFKDLEKIILHFIW-----NQKKPQIAKTLLSNKNKAGGITL 876

Query: 190 PKFKQMNEALLMKWIWRFY-NEDDSLWCKLINYKYKSLT 224
           P  +   +++++K  W ++ N +  +W ++ N +    T
Sbjct: 877 PDLRLYYKSIVIKTAWYWHKNREVDVWNRIENQEMDPAT 910

BLAST of Spg025336 vs. ExPASy TrEMBL
Match: A0A5A7TES3 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00040 PE=4 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 8.1e-87
Identity = 180/541 (33.27%), Postives = 273/541 (50.46%), Query Frame = 0

Query: 4   GSFNLPSIV-EDDILIFLQDDDNVLDNMLYIIKSFEQASGLKINLSKSTVSGINVTDQRV 63
           G+ NL  ++  DDIL+F++DD++ + N+  II  F+ ASGL INL+KST+S INV   R 
Sbjct: 3   GNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLASGLSINLNKSTISPINVDAART 62

Query: 64  SMIASKWGCSFQALPISYLGMPLGGKPRSSSFWDPLVEKIHRKIDNWRFASLSKGGRLTL 123
             IAS+WG S + LPI+YLG+PLGGK  + +FW  + EKI++K+ +W+++ LSKGG++TL
Sbjct: 63  EQIASQWGISTKFLPINYLGVPLGGKQITKTFWKNVEEKINKKLASWKYSMLSKGGKITL 122

Query: 124 LQSVLNFMPLYLSAVLKVPISVCKRIEKIFRKFLWDGIDKSSSSHLIRWDMVTMPKSEGG 183
           ++S L  +P Y  ++ K P+S CK IEK +R FLW    ++   HL+ W  +T PK  GG
Sbjct: 123 IKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNPPETHKLHLVNWAKITSPKERGG 182

Query: 184 LGLPKFKQMNEALLMKWIWRFYNEDDSLWCKLINYKYKSLTPGSIPSISKYASSRSPWMA 243
           LG+ + K  N ALL KW+WR+ +ED  LW K+IN KY+SL+ G IP +  ++SSRSPW +
Sbjct: 183 LGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSSSRSPWFS 242

Query: 244 ISNLQDIFWANSRWELRDGRRILFWFDRWTGSAPLRFVGNRFFSLAADKNICVEKAWMNT 303
           I    + F  +  W++++GR   FW   W  ++PL F   R F+L+ +K+  +   W NT
Sbjct: 243 ICKGLEWFQRHVSWKIKNGRSFSFWHSHWHQNSPLSFHYPRLFALSTNKDSSIRDMWNNT 302

Query: 304 NL-WNFFPRRPLLDRENSVWADFTASLPKLDLSNG------------------------- 363
            + W+  PRR L + E  +WA+   SL      NG                         
Sbjct: 303 LMDWDLNPRRQLREWEYPLWAELKNSLNASFCENGKDSPTWTLNSNGLYTVASVKKALQQ 362

Query: 364 ------------------------------------------------------------ 423
                                                                       
Sbjct: 363 PDQSLLDLQSQNTFKNLWKTSIPKKCIFFIWTLLYDSVNTAEQLMKRLPNLCSRPSWCVM 422

Query: 424 ------------------------ISSAIPLHAGC-----LCEEFLAKKALSQRDTIILN 429
                                   ISS +  +  C     LC    + K  ++++ I+ N
Sbjct: 423 CKRNDEDRIHLFILCPIAKSIWRLISSHLNSNVNCLSPKDLCITMCSWKQKTKKNVILYN 482

BLAST of Spg025336 vs. ExPASy TrEMBL
Match: A0A1S4E2K5 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo OX=3656 GN=LOC107991687 PE=4 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 8.9e-86
Identity = 178/541 (32.90%), Postives = 273/541 (50.46%), Query Frame = 0

Query: 4    GSFNLPSIV-EDDILIFLQDDDNVLDNMLYIIKSFEQASGLKINLSKSTVSGINVTDQRV 63
            G+ NL  ++  DDIL+F++DD++ + N+  II  F+ ASGL INL+KST+S INV   R 
Sbjct: 516  GNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLASGLSINLNKSTISPINVDASRT 575

Query: 64   SMIASKWGCSFQALPISYLGMPLGGKPRSSSFWDPLVEKIHRKIDNWRFASLSKGGRLTL 123
              IAS+WG S + LPI+YLG+PLGGK  + +FW  + EKI++K+ +W+++ LSKGG++TL
Sbjct: 576  EQIASQWGISTKFLPINYLGVPLGGKQITKTFWKNVEEKINKKLASWKYSMLSKGGKITL 635

Query: 124  LQSVLNFMPLYLSAVLKVPISVCKRIEKIFRKFLWDGIDKSSSSHLIRWDMVTMPKSEGG 183
            ++S L  +P Y  ++ KVP+S CK IEK +R FLW    ++   HL+ W  +T  K +GG
Sbjct: 636  IKSSLASLPTYQLSIFKVPVSTCKNIEKTWRNFLWKNPPETHKLHLVNWAKITSSKEKGG 695

Query: 184  LGLPKFKQMNEALLMKWIWRFYNEDDSLWCKLINYKYKSLTPGSIPSISKYASSRSPWMA 243
            LG+ + K  N ALL KW+WR+ +ED  LW K+IN KY+SL+ G IP +  ++SSRSPW +
Sbjct: 696  LGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSSSRSPWFS 755

Query: 244  ISNLQDIFWANSRWELRDGRRILFWFDRWTGSAPLRFVGNRFFSLAADKNICVEKAWMNT 303
            I    + F  +  W++++GR   FW   W  ++PL     R ++L+ +K   +   W NT
Sbjct: 756  ICKGLEWFQRHVSWKIKNGRSFSFWHSHWHQNSPLSSHYPRLYALSTNKESSIRDMWNNT 815

Query: 304  NL-WNFFPRRPLLDRENSVWADFTASLPKLDLSNG------------------------- 363
             + W+  PRR L + E+ +WA+   SL      NG                         
Sbjct: 816  LMDWDLNPRRQLREWEHPLWAELKNSLNASFCENGNDSPMWTLNSNGLYTVASVKKTLQQ 875

Query: 364  ------------------------------------------------------------ 423
                                                                        
Sbjct: 876  PEQNLLDFQSQNTFKNLWKTSIPKKCIFFIWTLLYDSVNTAEQLMKRLPNLCSRPSWCVM 935

Query: 424  ------------------------ISSAIPLHAGC-----LCEEFLAKKALSQRDTIILN 429
                                    ISS +  +  C     LC    + K  ++++ I+ N
Sbjct: 936  CKRNDEDRIHLFILCPIAKSIWRSISSHLSSNVNCLSPKELCITMCSWKQKTKKNVILFN 995

BLAST of Spg025336 vs. ExPASy TrEMBL
Match: A0A5D3DM72 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002870 PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 2.6e-85
Identity = 177/541 (32.72%), Postives = 272/541 (50.28%), Query Frame = 0

Query: 4    GSFNLPSIV-EDDILIFLQDDDNVLDNMLYIIKSFEQASGLKINLSKSTVSGINVTDQRV 63
            G+ NL  ++  DDIL+F++DD++ + N+  II  F+ ASGL INL+KST+S INV   R 
Sbjct: 516  GNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLASGLSINLNKSTISPINVDASRT 575

Query: 64   SMIASKWGCSFQALPISYLGMPLGGKPRSSSFWDPLVEKIHRKIDNWRFASLSKGGRLTL 123
              IAS+WG S + LPI+YLG+PLGGK  + +FW  + EKI++K+ +W+++ LSKGG++TL
Sbjct: 576  EQIASQWGISTKFLPINYLGVPLGGKQITKTFWKNVEEKINKKLASWKYSMLSKGGKITL 635

Query: 124  LQSVLNFMPLYLSAVLKVPISVCKRIEKIFRKFLWDGIDKSSSSHLIRWDMVTMPKSEGG 183
            ++S L  +P Y  ++ K P+S CK IEK +R FLW    ++   HL+ W  +T  K +GG
Sbjct: 636  IKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNPPETHKLHLVNWAKITSSKEKGG 695

Query: 184  LGLPKFKQMNEALLMKWIWRFYNEDDSLWCKLINYKYKSLTPGSIPSISKYASSRSPWMA 243
            LG+ + K  N ALL KW+WR+ +ED  LW K+IN KY+SL+ G IP +  ++SSRSPW +
Sbjct: 696  LGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSSSRSPWFS 755

Query: 244  ISNLQDIFWANSRWELRDGRRILFWFDRWTGSAPLRFVGNRFFSLAADKNICVEKAWMNT 303
            I    + F  +  W++++GR   FW   W  ++PL     R ++L+ +K   +   W NT
Sbjct: 756  ICKGLEWFQRHVSWKIKNGRSFSFWHSHWHQNSPLSSHYPRLYALSTNKESSIRDMWNNT 815

Query: 304  NL-WNFFPRRPLLDRENSVWADFTASLPKLDLSNG------------------------- 363
             + W+  PRR L + E+ +WA+   SL      NG                         
Sbjct: 816  LMDWDLNPRRQLREWEHPLWAELKNSLNASFCENGNDSPMWTLNSNGLYTVASVKKTLQQ 875

Query: 364  ------------------------------------------------------------ 423
                                                                        
Sbjct: 876  PEQNLLDFQSQNTFKNLWKTSIPKKCIFFIWTLLYDSVNTAEQLMKRLPNLCSRPSWCVM 935

Query: 424  ------------------------ISSAIPLHAGC-----LCEEFLAKKALSQRDTIILN 429
                                    ISS +  +  C     LC    + K  ++++ I+ N
Sbjct: 936  CKRNDEDRIHLFILCPIAKSIWRSISSHLSSNVNCLSPKELCITMCSWKQKTKKNVILFN 995

BLAST of Spg025336 vs. ExPASy TrEMBL
Match: A0A5A7TIB8 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00430 PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 2.6e-85
Identity = 185/541 (34.20%), Postives = 258/541 (47.69%), Query Frame = 0

Query: 5    SFNLPSIV-EDDILIFLQDDDNVLDNMLYIIKSFEQASGLKINLSKSTVSGINVTDQRVS 64
            + NL  I+  DDILIF++D ++ + N+  I+  FE ASGL INLSKST+  INV   R +
Sbjct: 497  NLNLTHILFADDILIFVEDKEDYVSNLKMILHLFESASGLNINLSKSTIFPINVPTDRAN 556

Query: 65   MIASKWGCSFQALPISYLGMPLGGKPRSSSFWDPLVEKIHRKIDNWRFASLSKGGRLTLL 124
             I   WG S   LP +YLGMPLGGKP SS+FWD +++KI +K+ +W+++ LSKGGR+TL+
Sbjct: 557  SIVDSWGISKGQLPTTYLGMPLGGKPSSSNFWDNILQKIQKKLSSWKYSQLSKGGRITLI 616

Query: 125  QSVLNFMPLYLSAVLKVPISVCKRIEKIFRKFLWDGIDKSSSSHLIRWDMVTMPKSEGGL 184
             S L  +P+Y  +V KVP  + ++IE  +R FLW+G     +  LIRW+ V  PK +GGL
Sbjct: 617  NSTLESLPIYQLSVFKVPKGIAQKIEAYWRNFLWNGTSNGHNISLIRWNQVVSPKEKGGL 676

Query: 185  GLPKFKQMNEALLMKWIWRFYNEDDSLWCKLINYKYKSLTPGSIPSISKYASSRSPWMAI 244
            G+      N ALL KW+W+F  E + LW +LI  KY     G  PS  KY+S+ SPW A+
Sbjct: 677  GIHSVHSTNFALLCKWLWKFLTEKEPLWKRLIISKYDQEKMGRFPSRGKYSSNNSPWKAV 736

Query: 245  SNLQDIFWANSRWELRDGRRILFWFDRWTGSAPLRFVGNRFFSLAADKNICVEKAWMNTN 304
            +N    F+ N  W++ DG  I FW D W G++PL  V  R F+L+ +K   V+  W N +
Sbjct: 737  TNCISWFYKNIGWKVNDGEDISFWLDNWNGNSPLSLVVPRLFALSTNKKGSVKDLW-NPS 796

Query: 305  L--WNFFPRRPLLDRENSVWADFTASLP--------------------------KLDLSN 364
            L  WN    RPL D E ++W +  ASLP                          K DLS 
Sbjct: 797  LKDWNIHVNRPLRDHEKNLWHNIKASLPTPLPDRGPSKPLWKLNSNNIFDTASIKKDLSE 856

Query: 365  GISSAIPLHA------------------------GC------------------------ 424
              +S    H                         GC                        
Sbjct: 857  ASASPTNFHPSLYKTLWKVDFPKKCKFFIWTLIHGCINTADRLQKRLPNWTLSPNWCYMC 916

Query: 425  ---------------------------------------LCEEFLAKKALSQRDTIILNI 430
                                                   L +   +    +Q+  I  N 
Sbjct: 917  NKSQEDINHLFIHCPYSQQLWSKAQALLKWNSTPNDVKSLAQNICSLNIKTQKGLITFNT 976

BLAST of Spg025336 vs. ExPASy TrEMBL
Match: A0A5D3C4J1 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00790 PE=4 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 9.9e-85
Identity = 178/541 (32.90%), Postives = 273/541 (50.46%), Query Frame = 0

Query: 4    GSFNLPSIV-EDDILIFLQDDDNVLDNMLYIIKSFEQASGLKINLSKSTVSGINVTDQRV 63
            G+ NL  ++  DDIL+F++DD++ + N+  II  F+ ASGL INL+KST+S INV   R 
Sbjct: 579  GNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLASGLSINLNKSTISPINVDASRT 638

Query: 64   SMIASKWGCSFQALPISYLGMPLGGKPRSSSFWDPLVEKIHRKIDNWRFASLSKGGRLTL 123
              IAS+WG S + LPI+YLG+PLGGK  + +FW  + EKI++K+ +W+++ LSKGG++TL
Sbjct: 639  EQIASQWGISTKFLPINYLGVPLGGKQTTKAFWKNVEEKINKKLASWKYSMLSKGGKITL 698

Query: 124  LQSVLNFMPLYLSAVLKVPISVCKRIEKIFRKFLWDGIDKSSSSHLIRWDMVTMPKSEGG 183
            ++S L  +P Y  ++ K P+S CK IEK +R FLW    ++   HL+ W  +T  K +GG
Sbjct: 699  IKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNPPETHKLHLVNWAKITSSKEKGG 758

Query: 184  LGLPKFKQMNEALLMKWIWRFYNEDDSLWCKLINYKYKSLTPGSIPSISKYASSRSPWMA 243
            LG+ + K  N ALL KW+WR+ +ED  LW K+IN KY+SL+ G IP +  ++SSRSPW +
Sbjct: 759  LGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSSSRSPWFS 818

Query: 244  ISNLQDIFWANSRWELRDGRRILFWFDRWTGSAPLRFVGNRFFSLAADKNICVEKAWMNT 303
            I    + F  +  W++++GR   FW   W  ++PL     R ++L+ +K   +   W NT
Sbjct: 819  ICKGLEWFQRHVSWKIKNGRSFSFWHGHWHQNSPLSSHYPRLYALSTNKESSIRDMWNNT 878

Query: 304  NL-WNFFPRRPLLDRENSVWADFTAS---------------------------------- 363
             + W+  PRR L + E  +WA+   S                                  
Sbjct: 879  LMDWDLNPRRQLREWELPLWAELKNSINASFCENGKDSPMWALNSNGLYTVASVKKALQQ 938

Query: 364  -----------------------------------------------LPKL--------- 423
                                                           LPKL         
Sbjct: 939  PEQSLLDLQSQNTFKNLWKTSIPKKCIFFIWTLLYDSVNTAEQLMKRLPKLCSRPSWCVM 998

Query: 424  -------------------DLSNGISSAIPLHAGC-----LCEEFLAKKALSQRDTIILN 429
                                +   ISS +  +  C     LC    + K  ++++ I+ N
Sbjct: 999  CKRNDEDRIHLFILCPIAKSIWRTISSHLNSNVNCLSPKELCITMCSWKQKTKKNVILFN 1058

BLAST of Spg025336 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 96.7 bits (239), Expect = 4.9e-20
Identity = 58/207 (28.02%), Postives = 94/207 (45.41%), Query Frame = 0

Query: 75  ALPISYLGMPLGGKPRSSSFWDPLVEKIHRKIDNWRFASLSKGGRLTLLQSVLNFMPLYL 134
           ALP+ YLG+PL  K  ++S + PLVEKI  +I  W    LS  GRL L+ SV++ +  + 
Sbjct: 22  ALPVRYLGLPLLTKKMTTSDYGPLVEKIRVRIGKWTARHLSFAGRLQLISSVIHSLTNFW 81

Query: 135 SAVLKVPISVCKRIEKIFRKFLWDGIDKSSSSHLIRWDMVTMPKSEGGLGLPKFKQMNE- 194
            +  ++P +  K I+ I   FLW G + ++    + W  V  PK EGGLG+   K+ N+ 
Sbjct: 82  MSAFRLPSACIKEIDSICSSFLWSGPELNTKKAKVAWSDVCTPKDEGGLGIRSLKEANKG 141

Query: 195 --------ALLMKWIWRFYNEDDSLWCKLINYKYKSLTPGSIPSISKYASSRSPWMAISN 254
                     L  W+W+          K++  K+++L  G +                  
Sbjct: 142 SFWSISGNTTLGSWMWK----------KIL--KHRALASGFV------------------ 189

Query: 255 LQDIFWANSRWELRDGRRILFWFDRWT 273
                    + ++ +G    FWFD W+
Sbjct: 202 ---------KHDIHNGSNTSFWFDNWS 189

BLAST of Spg025336 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 74.3 bits (181), Expect = 2.6e-13
Identity = 43/148 (29.05%), Postives = 69/148 (46.62%), Query Frame = 0

Query: 130 MPLYLSAVLKVPISVCKRIEKIFRKFLWDGIDKSSSSHLIRWDMVTMPKSEGGLGLPKFK 189
           +P Y  A   +P +VCK+I  +   F W    ++   H   WD ++  K+EGG+G    +
Sbjct: 3   LPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDIE 62

Query: 190 QMNEALLMKWIWRFYNEDDSLWCKLINYKY-KSLTPGSIPSISKYASSRSPWMAISNLQD 249
             N ALL K +WR  +  +SL  K+   +Y     P + P  S+       W +I   Q+
Sbjct: 63  AFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSR---PSFVWKSIHASQE 122

Query: 250 IFWANSRWELRDGRRILFWFDRWTGSAP 277
           I    +R  + +G  I+ W  +W  S P
Sbjct: 123 ILRQGARAVVGNGEDIIIWRHKWLDSKP 147

BLAST of Spg025336 vs. TAIR 10
Match: AT3G25720.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 59.3 bits (142), Expect = 8.7e-09
Identity = 35/110 (31.82%), Postives = 53/110 (48.18%), Query Frame = 0

Query: 174 VTMPKSEGGLGLPKFKQMNEALLMKWIWRFYNEDDSLWCKLINYKYKSLTPGSIPSISKY 233
           V +PK+EGGLGL  F + N  L +K +WR ++   SLW     ++Y  L      S+SK+
Sbjct: 18  VCLPKAEGGLGLRWFSEWNTTLNLKLVWRLFSGGGSLWVDW--HRYHHLQGLGDASVSKF 77

Query: 234 ASSRS------PWMAISNLQDIFWANSRWELRDGRRILFWFDRWTGSAPL 278
            +S+        W  +  L+ +     R  + +G    FW D WT   PL
Sbjct: 78  WTSQELVSDSWNWKCLLRLRPLAERFLRCNIGNGLTARFWTDNWTPFGPL 125