Spg025332 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025332 vs. NCBI nr
Match: XP_024190234.1 (uncharacterized protein LOC112194221 [Rosa chinensis])

HSP 1 Score: 153.7 bits (387), Expect = 3.4e-33
Identity = 105/389 (26.99%), Postives = 176/389 (45.24%), Query Frame = 0

Query: 6    DEIIWKYEDKGSFSVKSAYHLAKSLSSKDIAS-GSSDASSK---SIWKSIWKANCIPIAK 65
            D I+W Y   G ++VKS   LA  L      S G S++S+K    +W ++WK       K
Sbjct: 853  DSIVWHYCKDGRYTVKSGCWLASELQRVHEGSVGCSNSSNKEGTQVWDTLWKLRIANKVK 912

Query: 66   ITVWKILNDALPTKTNIAKKGIHTNLCCCLCRNGKESTAHIFWICKFSKKLWADFIPNAS 125
            + +W+     LP   N+ ++ +  +  CC C   +E+T H  W C +SKK+W     N  
Sbjct: 913  LFLWRACKGFLPCAANLFRRHVGNSSLCCRCGREEETTIHCMWSCPWSKKVWKFSFLNGV 972

Query: 126  PLWLRCRDWEKP--IDFWNFIQRNLSKEETRIAILLLWHIWDARNTSNINNYLPDINRVK 185
                  + W +P  ID +  + +  SKEE  +  ++ W +W +RN       + +   + 
Sbjct: 973  -----YKQWREPSFIDLFAHVVKTSSKEEVELFSVICWLLWKSRNRFKHEGVVEESKHIV 1032

Query: 186  RRIATSIDERPNLQEDNQNIS-ELKSLSSHLHWEPPDPGCWKLNADASWSEKDEIGGIGW 245
                  ++   + Q  N+N++ +       + W PP     KLN DA+   K +   +G 
Sbjct: 1033 WSANEWLNNFQDAQRSNENVTKDATQKRVKVKWRPPTAPKLKLNTDAAIDYKKKKADLGM 1092

Query: 246  AIRDSNGSLIGLGCKKISKLWNIKCLEAKAIIEGLKAYEDSGGFEGSRRKPPLVVESDSV 305
             +RD  G L   G K +    +I  +EA A+  GL  Y ++ GF+       LVVESDS 
Sbjct: 1093 VVRDGEGKLKCAGHKVLPGNPSIVAIEALAVYHGLLLYREA-GFQN------LVVESDST 1152

Query: 306  EVVNAVNRVADDATELCLFVDEIDSFRRSNRVRSFSKCSRQSNSLAHELARAAAKNGDFL 365
             V++A+N+   D +     +D+I     +     + K +R+ N  AH++A+ A       
Sbjct: 1153 IVIDALNKTELDLSVEGGVLDDIKCLASTFESVPWKKVNREGNIAAHKMAKHA------- 1212

Query: 366  FFVNFSLLYGEEDRFWREVPFPEWCRSVV 388
                   L  +EDR W+E   P W   ++
Sbjct: 1213 -------LMADEDRLWQEAG-PPWLTGII 1214

BLAST of Spg025332 vs. NCBI nr
Match: XP_042939495.1 (uncharacterized protein LOC122274525 [Carya illinoinensis])

HSP 1 Score: 144.4 bits (363), Expect = 2.0e-30
Identity = 106/381 (27.82%), Postives = 167/381 (43.83%), Query Frame = 0

Query: 2    SGSKDEIIWKYEDKGSFSVKSAYHLAKSLSSKDIASGSSDASSKSIWKSIWKANCIPIAK 61
            SG  D+ IW Y   G FSVKSAYHL   +S K    G S       WK +W  N   + K
Sbjct: 1051 SGLPDKQIWGYTKDGFFSVKSAYHL--EVSRKRREKGESSEGHSFNWKKLWGLNVPRVVK 1110

Query: 62   ITVWKILNDALPTKTNIAKKGIHTNLCCCLCRNGKESTAHIFWICKFSKKLWADFIPNAS 121
            + +WK LND LPT+TN+ K+ +  + CC +     ES  H  W C+ S  +WAD     S
Sbjct: 1111 VFLWKALNDCLPTRTNLKKRKVVKDACCPIYETEDESITHALWSCRGSMDVWAD---KKS 1170

Query: 122  PLWLRCRDWEKPIDFWNFIQRNLSKEETRIAILLLWHIWDARNTSNINNYLPDINRVKRR 181
            PL     +  +  + W  +   ++KEE  + ++++  +W  RN     N   D   +  +
Sbjct: 1171 PLHKWSSNEVQFYELWERLSALVAKEELELVVVVMRGVWFRRNAFIFENKFTDPGTIVEQ 1230

Query: 182  IATSIDERPNLQEDNQ-NISELKSLSSHLHWEPPDPGCWKLNADASWSEKDEIGGIGWAI 241
             A S++     Q   +  +           W+ P   C K+N DAS ++KDE  GIG  +
Sbjct: 1231 AAISLENFQTSQTIKKGGMGSNGKGRQGCKWKAPIGECIKVNWDASINKKDERVGIGVVV 1290

Query: 242  RDSNGSLIGLGCKKISKLWNIKCLEAKAIIEGLKAYEDSGGFEGSRRKPPLVVESDSVEV 301
            RD  G ++   C       +    E +A+   +K   +   FE       ++ E D++ V
Sbjct: 1291 RDGRGEVMVSLCYSNEGCCSPVVAELRALWRAMKLCAEL-NFEN------VIFEGDALVV 1350

Query: 302  VNAVNRVADDATELCLFVDEIDSFRRSNRVRSFSKCSRQSNSLAHELARAAAKNGDFLFF 361
            VNAVN   +        V+++    +  +        R+ N +AH L +           
Sbjct: 1351 VNAVNSEKESWEWYGQMVEDMKGVLKIRQRWKVQHVFRECNQVAHLLVK----------- 1405

Query: 362  VNFSLLYGEEDRFWREVPFPE 382
            V+F+     E++ W E   PE
Sbjct: 1411 VSFTF---NEEQIWMECCPPE 1405

BLAST of Spg025332 vs. NCBI nr
Match: XP_023915006.1 (uncharacterized protein LOC112026546 [Quercus suber])

HSP 1 Score: 144.1 bits (362), Expect = 2.7e-30
Identity = 102/352 (28.98%), Postives = 161/352 (45.74%), Query Frame = 0

Query: 5   KDEIIWKYEDKGSFSVKSAYHLAKS-LSSKDIASGSSDASSKSIWKSIWKANCIPIAKIT 64
           +D ++W    KGSF+VKSAY++A S L S +    SS      +WK IW     P  KI 
Sbjct: 148 EDCLVWVGNKKGSFTVKSAYYIASSILDSNEDGESSSSNEMTGLWKRIWHQKIPPKLKIF 207

Query: 65  VWKILNDALPTKTNIAKKGIHTNLCCCLCRNGKESTAHIFWICKFSKKLWADFIPNASPL 124
            W++  + LPT  N++ +G+H +  C LC    E+TAH    C  +K  WA +    + L
Sbjct: 208 AWRLCVNGLPTMFNLSHRGVHCSCFCPLCDKAIETTAHALLHCDHAKLTWAHWHNGFAVL 267

Query: 125 WLRCRDWEKPIDFWNFIQRNLSKEETRIAILLLWHIWDARNTSNINNYLPDINRVKRRIA 184
                  ++P+D    I +N S     + I + W IW  RN +         +++     
Sbjct: 268 ---SAGTQEPVDIALDIIKNDSSSALELFIAVAWSIWWNRNQALHEGSGAPPSQIWDMAI 327

Query: 185 TSIDERPNLQEDNQNISELKSLSSHLHWEPPDPGCWKLNADASWSEKDEIGGIGWAIRDS 244
             + E     ++  + S L  +SS ++W PP  G  K+N D + S+       G  IRDS
Sbjct: 328 RILGE----YKEACSFSNLPLVSSLINWRPPPAGFTKINVDGAASDDGRPSSTGVIIRDS 387

Query: 245 NGSLIGLGCKKISKLWNIKCLEAKAIIEGLKAYEDSGGFEGSRRKPPLVVESDSVEVVNA 304
            GS I   C+ +S  +     EA A+ +G+    + G          ++ ESD++ ++ A
Sbjct: 388 QGSSIAASCRVLSSPFPADISEALALQDGVMLALELG-------LSKVIFESDALSIIQA 447

Query: 305 VNRVADDATELCLFVDEIDSFRRSNRVRSFSKCSRQSNSLAHELARAAAKNG 356
           +N   +   E+   +  I     S    +F    R+ N  AHELARAA   G
Sbjct: 448 INE-GNVGGEIGHIMQNIKDLASSFSWCTFQHQKREGNRAAHELARAARMTG 484

BLAST of Spg025332 vs. NCBI nr
Match: XP_027118368.1 (uncharacterized protein LOC113735569 [Coffea arabica])

HSP 1 Score: 142.5 bits (358), Expect = 7.7e-30
Identity = 102/360 (28.33%), Postives = 177/360 (49.17%), Query Frame = 0

Query: 2    SGSKDEIIWKYEDKGSFSVKSAYHLAKSLSS--KDIASGSSDASSKSIWKSIWKANCIPI 61
            S + D++IW Y   GSF+VKSAYHL K+ S+  +D+ASG S+ S + IW  +WK N    
Sbjct: 796  SHNHDKLIWHYSKNGSFTVKSAYHLIKTQSTGWQDVASG-SNGSVQDIWPRLWKLNIPNK 855

Query: 62   AKITVWKILNDALPTKTNIAKKGIHTNLCCCLCRNGKESTAHIFWICKFSKKLWADFIPN 121
             KI +W+I    LPT + +  +G+H +  C LC+   E   H+F  C  + + WA     
Sbjct: 856  VKIFLWRICKGILPTSSTLNSRGVHLDPMCLLCKTPVEDVTHLFLCCPVAVQAWA----- 915

Query: 122  ASPLWLRCR-DWEKPIDFW-NFIQRNLSKEETRIAILLLWHIWDARNTSNINNYLPDINR 181
             S L L+ R D  K +  W   +   L K++  +  ++LW++W  RN +  +    D   
Sbjct: 916  LSFLTLKIRLDSAKQVSSWLPGLLNLLKKQDMELVAIILWNLWLNRNGALFDGSYRDPLS 975

Query: 182  VKRRIATSIDERPNLQEDNQN-ISELKSLSSHLHWEPPDPGCWKLNADASWSEKDEIGGI 241
            +   ++ SI+     +E N +  + +++  + LHW  P  G  K N D +   +    G+
Sbjct: 976  L---VSLSINFLQKYREANTSPNTPIRTSIAPLHWTKPPVGFLKANFDGAVFSEYGYSGV 1035

Query: 242  GWAIRDSNGSLIGLGCKKISKLWNIKCLEAKAIIEGLKAYEDSGGFEGSRRKPPLVVESD 301
            G  +RD  G+ +     KIS +++ + +EA A    +             + P +++E D
Sbjct: 1036 GVVVRDERGNFVTGTSHKISGIFSPEVIEAYAARTAISLLL-------QWKVPNIILEGD 1095

Query: 302  SVEVVNAVNRVADDATELCLFVDEIDSFRRSNRVRSFSKCSRQSNSLAHELARAAAKNGD 357
            S+++VN +  +  D +   + +D+I    ++      +   R++N  AH LAR A    D
Sbjct: 1096 SLKIVNMLKLMESDDSAYGVLLDDIFVRLQNFAAWEANWVPRKANVPAHLLARNARSISD 1139

BLAST of Spg025332 vs. NCBI nr
Match: XP_021847414.1 (uncharacterized protein LOC110787151 [Spinacia oleracea])

HSP 1 Score: 141.0 bits (354), Expect = 2.3e-29
Identity = 99/348 (28.45%), Postives = 160/348 (45.98%), Query Frame = 0

Query: 4    SKDEIIWKYEDKGSFSVKSAYHLAKSLSSKDIASGSSDASSKSIWKSIWKANCIPIAKIT 63
            ++D + W+ E  G +SV+SAY        + +   SS+ S  S+WK IWK N +P  K+ 
Sbjct: 822  AEDRVCWELESDGKYSVRSAYRALFMDDWRRMEVSSSNPS--SLWKQIWKLNVLPRVKVF 881

Query: 64   VWKILNDALPTKTNIAKKGIHTNLCCCLCRNGKESTAHIFWICKFSKKLWADFIPNASPL 123
             W+  ++ALPT+  ++K+    +  C +C    ES  H    CK ++ +W +        
Sbjct: 882  AWRACHEALPTRKGLSKRVAGYDAVCGVCHCEDESVIHAVRECKLARAIWCESRFEHMLS 941

Query: 124  WLRCRDWEKPIDFWNFIQRNLSKEETRIAILLLWHIWDARNTSNINNYLPDINRVKRRIA 183
            W  C      +D+W+   + L  E     I L W +W ARN + +     D     +   
Sbjct: 942  WQCC----SLVDWWDRGFKELDSEGMEEFITLCWAVWGARNKAVMEGLTSDPTSTVKYAT 1001

Query: 184  TSIDERPNLQEDNQNISELKSLSSHLHWEPPDPGCWKLNADASWSEKDEIG-GIGWAIRD 243
            T  +E           S        + W  P+ G +K+N DA      E+G G+G  IRD
Sbjct: 1002 TVCNELRQEGVKGSRGSSGGGAEWAVEWSKPEEGWYKVNVDAGL--LGEVGSGMGAVIRD 1061

Query: 244  SNGSLIGLGCKKISKLWNIKCLEAKAIIEGLKAYEDSGGFEGSRRKPPLVVESDSVEVVN 303
              G ++    ++  + W     EAKA++ G++   + G          +VVESD + V+ 
Sbjct: 1062 DEGGVVSCAVRQGCEKWATNVAEAKAVLFGMELCAELG-------MQRVVVESDCLGVIQ 1121

Query: 304  AVNRVADDATELCLFVDEIDSFRRS--NRVRSFSKCSRQSNSLAHELA 349
            A+ R A   +E  L V++I +F  +  N V SF K  R  N +AH+LA
Sbjct: 1122 ALRREASGCSEFWLVVEDIMAFCSNFLNVVWSFVK--RSGNKVAHDLA 1152

BLAST of Spg025332 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.3e-08
Identity = 75/358 (20.95%), Postives = 139/358 (38.83%), Query Frame = 0

Query: 1   MSGSKDEIIWKYEDKGSFSVKSAYHLAKSLSSKDIASGSSDASSKSIWKSIWKANCIPIA 60
           ++G++D + WK+   G FSV+SAY +   L+  ++       +  S +  +WK       
Sbjct: 248 VTGARDRLSWKFSQDGQFSVRSAYEM---LTVDEVPR----PNMASFFNCLWKVRVPERV 307

Query: 61  KITVWKILNDALPTKTNIAKKGIHTNLCCCLCRNGKESTAHIFWICKFSKKLWADFIPNA 120
           K  +W + N A+ T+    ++ +  +  C +C+ G ES  H+   C     +W   +P  
Sbjct: 308 KTFLWLVGNQAVMTEEERHRRHLSASNVCQVCKGGVESMLHVLRDCPAQLGIWVRVVPQR 367

Query: 121 SPLWLRCRDWEKPIDFW---NFIQRNLSKE---ETRIAILLLW-HIWDARNTSNINNYLP 180
                    + K +  W   N   R+  ++    T  A+++ W   W   N    N    
Sbjct: 368 RQQGF----FSKSLFEWLYDNLGDRSGCEDIPWSTIFAVIIWWGWKWRCGNIFGENTKCR 427

Query: 181 DINRVKRRIATSIDERPNLQEDNQNISELKSLSSHLHWEPPDPGCWKLNADASWSEKDEI 240
           D  +  +  A  +  R +       I++ + +   + W  P  G  K+N D +      +
Sbjct: 428 DRVKFVKEWAVEV-YRAHSGNVLVGITQPR-VERMIGWVSPCVGWVKVNTDGASRGNPGL 487

Query: 241 GGIGWAIRDSNGSLIGLGCKKISKLWNIKCLEAKAIIEGLKAYEDSGGFEGSRRKPPLVV 300
              G  +RD  G+  G     I +  +    E   +  GL        F   ++ P + +
Sbjct: 488 ASAGGVLRDCTGAWCGGFSLNIGRC-SAPQAELWGVYYGLY-------FAWEKKVPRVEL 547

Query: 301 ESDSVEVVNAVNRVADDATELCLFVDEIDSFRRSNRVRSFSKCSRQSNSLAHELARAA 352
           E DS  +V  +     D+  L   V     F + + +       R++N LA  LA  A
Sbjct: 548 EVDSEVIVGFLKTGISDSHPLSFLVRLCHGFLQKDWLVRIVHVYREANRLADGLANYA 584

BLAST of Spg025332 vs. ExPASy TrEMBL
Match: A0A2N9H3I8 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS34205 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 2.0e-31
Identity = 103/350 (29.43%), Postives = 165/350 (47.14%), Query Frame = 0

Query: 6   DEIIWKYEDKGSFSVKSAYHLAKSLSSKDIASGSSDASSKSIWKSIWKANCIPIAKITVW 65
           D +IW    +G FSVKSAYHL  SL +   A+ SS  S  SIW SIW     P  K+ VW
Sbjct: 552 DSLIWIVTKRGVFSVKSAYHLQLSLRNSQEAATSSRTSVHSIWSSIWAVKVPPKVKLFVW 611

Query: 66  KILNDALPTKTNIAKKGIHTNLCCCLCRNGKESTAHIFWICKFSKKLWADFIPNASPLWL 125
           K  +D LPT+T + +KGI  +  C  C    E+  H+ W C+F+ K+W       S  + 
Sbjct: 612 KACHDILPTRTKLFEKGISHSYSCLWCAEEPETCDHVLWGCEFAHKVWKSSSVAISSQYS 671

Query: 126 RCRDWEKPIDFWNFIQRNLSKEETRIAILLLWHIWDARNTSNINNYLPDINRVKRRIA-T 185
            C  ++   DF   +   L      IA    W +W ARN +     +P ++ + + +A  
Sbjct: 672 LCLHFK---DFIGTLILELPSPALEIAFTTAWALWKARNETCWEGKIPVVDAICQEVACI 731

Query: 186 SIDERPNLQEDNQNISELKSLSSHLHWEPPDPGCWKLNADASWSEKDEIGGIGWAIRDSN 245
           ++D    L+ +++ + ++  L     W+ P  G  KLN    +S      G G  IRD+ 
Sbjct: 732 ALD---FLEANSKVLGDMAGLGGSSRWQKPPSGAVKLNLACCFSLNAAKVGAGLLIRDTG 791

Query: 246 GSLIGLGCKKISKLWNIKCLEAKAIIEGLKAYEDSGGFEGSRRKPPLVVESDSVEVVNAV 305
           G ++ +    I    ++    A+A+++ ++   D     G RR   LVVE D++E+ N +
Sbjct: 792 GIVMAVLEDSIQACGDLLQSHARAVLKAVQFAFD----VGFRR---LVVEMDNLELCNLL 851

Query: 306 NRVADDATELCLFVDEIDSFRRSNRVRSFSKCSRQSNSLAHELARAAAKN 355
                    + + V++I S+     + SF    +  N  AH LA  AA +
Sbjct: 852 QAGLPCLAPIGVLVEDICSWALCFNMLSFVFSKKICNKAAHALATEAASS 888

BLAST of Spg025332 vs. ExPASy TrEMBL
Match: A0A7C9A7V4 (Uncharacterized protein OS=Opuntia streptacantha OX=393608 PE=4 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 3.4e-31
Identity = 106/353 (30.03%), Postives = 161/353 (45.61%), Query Frame = 0

Query: 5   KDEIIWKYEDKGSFSVKSAYHLAKSLSSKDIASGSSDASS--KSIWKSIWKANCIPIAKI 64
           +D +IW     G F+V+SA  LA+ +  +   +  +  S      W  IW A   P AK 
Sbjct: 84  EDTLIWDSTPSGVFTVRSATMLARRIEMQQQLNNEASCSGDFDGRWARIWTALATPRAKN 143

Query: 65  TVWKILNDALPTKTNIAKKGIHTNLCCCLCRNGKESTAHIFWICKFSKKLWADFIPNASP 124
             W+   DALPT  N+ K+G+  ++ C +C NG E+T HIF  C  + + W       SP
Sbjct: 144 LCWRACRDALPTCVNLYKRGVEIDVICPVCGNGYETTTHIFLDCDLAAEYW-----RKSP 203

Query: 125 LWLRCRD-WEKPIDFW-NFIQRNLSKEETRIAILLLWHIWDARNTSNINNYLPDINRVKR 184
                RD  E     W +   + L K +  +   LLW +W  RN    +    D+     
Sbjct: 204 FRFCTRDRHEHDFGSWCHGTTKLLDKAQCGLLATLLWGLWLTRNRWVFDRKREDVGLTML 263

Query: 185 RIATSIDERPNLQEDNQNISELKSLSSHLHWEPPDPGCWKLNADASWSEKDEIGGIGWAI 244
           R   S +    + + N      K     L WEPP  G  K+N DAS + +D + G+G  +
Sbjct: 264 RCVDSWNHYVEVLQANGERGGRKQ-GECLTWEPPRQGQLKVNVDAS-TGRDGMKGVGVVV 323

Query: 245 RDSNGSLIGLGCKKISKLWNIKCLEAKAIIEGLKAYEDSGGFEGSRRKPPLVVESDSVEV 304
           RD NG +  + C+     W ++ +EA A++ GL+     G          L +E+DS +V
Sbjct: 324 RDKNGDVKIMACRSFRANWEVETVEAYAVLYGLQICWQEGFH-------TLELETDSKQV 383

Query: 305 VNAVNRVADDATELCLFVDEIDSFRRSNRVRSFSKCSRQSNSLAHELARAAAK 354
            +A+N   D       F+ +  +      V SFS  SR +N +AHELAR A +
Sbjct: 384 ADALNGKKDFLNYTSTFIHDALNLGTRIPVISFSFVSRVTNRVAHELARLALR 422

BLAST of Spg025332 vs. ExPASy TrEMBL
Match: A0A803PAX6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.3e-30
Identity = 92/353 (26.06%), Postives = 169/353 (47.88%), Query Frame = 0

Query: 4    SKDEIIWKYEDKGSFSVKSAYHLAKSLSSKDIASGSSDASSKSIWKSIWKANCIPIAKIT 63
            S D++IW +   G ++V+S Y +A+  + K  A  SS+A+++  W SIWK    P  +  
Sbjct: 965  SNDDLIWHFTSDGLYTVRSGYEVAEIDNYK--AGPSSEATTRKWWTSIWKIEAPPKIRNF 1024

Query: 64   VWKILNDALPTKTNIAKKGIHTNLCCCLCRNGKESTAHIFWICKFSKKLWADFIPNASPL 123
            +W++ N  +P  T + ++G++ N  C  C   +E+  H  W C +SK++W  +     P 
Sbjct: 1025 LWRVCNGWIPVNTILQRRGMNINTSCYWCGQAEETIEHCLWFCPYSKQIWKKY-----PW 1084

Query: 124  WLRCRDWEKP-IDFWNFIQRNLSKEETRIAILLLWHIWDARNTSNINNYLPD----INRV 183
            W   +  +   ID    I+  ++KEE    I++ W IW+ RN   +N+++      +   
Sbjct: 1085 WKVLKQSKGTIIDILINIRNQVAKEEFNFFIIMSWLIWNRRNKKRLNHFMSSQESWLKWA 1144

Query: 184  KRRIATSIDERPNLQEDNQNISELKSLSSHLHWEPPDPGCWKLNADASWSEKDEIGGIGW 243
            +  +   + + P L+  N     L     HL WEPP    + +N+DAS    +   G+G 
Sbjct: 1145 QMEVDYIMHQDPKLESHN-----LTKPLKHLGWEPPPVNIFCINSDASVHYTEAEIGLGV 1204

Query: 244  AIRDSNGSLIGLGCKKISKLWNIKCLEAKAIIEGLKAYEDSGGFEGSRRKPPLVVESDSV 303
             IR + G +     + ++ +++I+  EA AI  G+     +          P +++SD +
Sbjct: 1205 VIRTNKGEVTAAATQHVNGVFSIELAEALAIQMGISLAVQTSAI-------PFIIQSDCL 1264

Query: 304  EVVNAVNRVADDATELCLFVDEIDSFRRSNRVRSFSKCSRQSNSLAHELARAA 352
             V+  +       T+    +DE+ S       ++ S  SR +N +AH LA+ A
Sbjct: 1265 RVIEYIKGKFQAKTDWGALLDEV-SDPAFVHCQAASHTSRNNNRVAHSLAKLA 1297

BLAST of Spg025332 vs. ExPASy TrEMBL
Match: A0A6P6WSG1 (uncharacterized protein LOC113735569 OS=Coffea arabica OX=13443 GN=LOC113735569 PE=4 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 3.7e-30
Identity = 102/360 (28.33%), Postives = 177/360 (49.17%), Query Frame = 0

Query: 2    SGSKDEIIWKYEDKGSFSVKSAYHLAKSLSS--KDIASGSSDASSKSIWKSIWKANCIPI 61
            S + D++IW Y   GSF+VKSAYHL K+ S+  +D+ASG S+ S + IW  +WK N    
Sbjct: 796  SHNHDKLIWHYSKNGSFTVKSAYHLIKTQSTGWQDVASG-SNGSVQDIWPRLWKLNIPNK 855

Query: 62   AKITVWKILNDALPTKTNIAKKGIHTNLCCCLCRNGKESTAHIFWICKFSKKLWADFIPN 121
             KI +W+I    LPT + +  +G+H +  C LC+   E   H+F  C  + + WA     
Sbjct: 856  VKIFLWRICKGILPTSSTLNSRGVHLDPMCLLCKTPVEDVTHLFLCCPVAVQAWA----- 915

Query: 122  ASPLWLRCR-DWEKPIDFW-NFIQRNLSKEETRIAILLLWHIWDARNTSNINNYLPDINR 181
             S L L+ R D  K +  W   +   L K++  +  ++LW++W  RN +  +    D   
Sbjct: 916  LSFLTLKIRLDSAKQVSSWLPGLLNLLKKQDMELVAIILWNLWLNRNGALFDGSYRDPLS 975

Query: 182  VKRRIATSIDERPNLQEDNQN-ISELKSLSSHLHWEPPDPGCWKLNADASWSEKDEIGGI 241
            +   ++ SI+     +E N +  + +++  + LHW  P  G  K N D +   +    G+
Sbjct: 976  L---VSLSINFLQKYREANTSPNTPIRTSIAPLHWTKPPVGFLKANFDGAVFSEYGYSGV 1035

Query: 242  GWAIRDSNGSLIGLGCKKISKLWNIKCLEAKAIIEGLKAYEDSGGFEGSRRKPPLVVESD 301
            G  +RD  G+ +     KIS +++ + +EA A    +             + P +++E D
Sbjct: 1036 GVVVRDERGNFVTGTSHKISGIFSPEVIEAYAARTAISLLL-------QWKVPNIILEGD 1095

Query: 302  SVEVVNAVNRVADDATELCLFVDEIDSFRRSNRVRSFSKCSRQSNSLAHELARAAAKNGD 357
            S+++VN +  +  D +   + +D+I    ++      +   R++N  AH LAR A    D
Sbjct: 1096 SLKIVNMLKLMESDDSAYGVLLDDIFVRLQNFAAWEANWVPRKANVPAHLLARNARSISD 1139

BLAST of Spg025332 vs. ExPASy TrEMBL
Match: A0A803QH76 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 6.4e-30
Identity = 114/396 (28.79%), Postives = 177/396 (44.70%), Query Frame = 0

Query: 6    DEIIWKYEDKGSFSVKSAYHLAKSLSSKDIASGSSDASSKSIWKSIWKANCIPIAKITVW 65
            D+I+W + D G ++V+S YHLA SL S+D    SS ++++  W  +W        KI  W
Sbjct: 1333 DKIMWHHSDSGCYTVQSGYHLAVSLDSRD--DHSSSSTNRKWWNRLWSLQLPKKVKIFAW 1392

Query: 66   KILNDALPTKTNIAKKGIHTNLCCCLCRNGKESTAHIFWICKFSKKLWAD-----FIPNA 125
            +++NDALPT  N+A + I ++  C LC+   ES  H  + C  +K +W       FIPN 
Sbjct: 1393 RLVNDALPTAVNLAHRKIASSAACALCKCSWESVGHALFRCHRAKLVWDKFHFNVFIPNI 1452

Query: 126  SPLWLRCRDWEKPIDFWNFIQRNLSKEETRIAILLLWHIWDARNTSNINNYLPDINRVKR 185
            + +        K  D ++ +    +  +  I   LLW IW  RN   I+   P    +  
Sbjct: 1453 ANI--------KGFDIYSHLAAAHNDNDLEIITCLLWCIWSERN-KEIHGTKPKPAPILC 1512

Query: 186  RIATSIDERPNLQEDNQN-ISELKSLSSHL------------HWEPPDPGCWKLNADASW 245
              A +   +  +    Q   S L S S +              W+PPD GC+KLN DA+ 
Sbjct: 1513 SFAANYLTQYKMAATQQKCASGLSSTSCNASVPVDAPEKTRPQWKPPDEGCYKLNVDAAC 1572

Query: 246  SEKDEIGGIGWAIRDSNGSLIGLGCKKISKLWNIKCLEAKAIIEGLKAYEDSGGFEGSRR 305
            +E   + G G  +RD +G +I    K     ++ K +E  A+   L+       +     
Sbjct: 1573 TEAGAVIGFGAILRDHHGFVIAGLSKPFPGTFSPKEMEVTALFHSLQ-------WALQLH 1632

Query: 306  KPPLVVESDSVEVVNAVNRVADDATELCLFVDEID--SFRRSNRVR-SFSKCSRQSNSLA 365
             P   +E+D++ VVNA+N+ A     L  F D ++  S+  S   R   S   R +N  A
Sbjct: 1633 LPISFIETDALVVVNALNK-ASSTKFLAPFYDLVEDVSYLLSFFPRVKVSHVKRDANEAA 1692

Query: 366  HELARAAAKNGDFLFFVNFSLLYGEEDRFWREVPFP 381
            H LA             NF+L   E+  +  E P P
Sbjct: 1693 HALA-------------NFALRLDEDYTWLGETPSP 1696

BLAST of Spg025332 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 100.5 bits (249), Expect = 3.1e-21
Identity = 90/353 (25.50%), Postives = 151/353 (42.78%), Query Frame = 0

Query: 2   SGSKDEIIWKYEDKGSFSVKSAYHLAKSLSSKDIASGSSDASSKSIWKSIWKANCIPIAK 61
           S   D+IIW Y   G ++V+S Y L     S +I + +    S  +   IW    +P  K
Sbjct: 113 SKKPDKIIWNYNTTGEYTVRSGYWLLTHDPSTNIPAINPPHGSIDLKTRIWNLPIMPKLK 172

Query: 62  ITVWKILNDALPTKTNIAKKGIHTNLCCCLCRNGKESTAHIFWICKFSKKLWADFIPNAS 121
             +W+ L+ AL T   +  +G+  +  C  C    ES  H  + C F+   W     +  
Sbjct: 173 HFLWRALSQALATTERLTTRGMRIDPSCPRCHRENESINHALFTCPFATMAWRLSDSSLI 232

Query: 122 PLWLRCRDWEKPI-DFWNFIQ-RNLSKEETRIAILLLWHIWDARNTSNINNYLPDINR-V 181
              L   D+E+ I +  NF+Q   +S     + + L+W IW ARN    N +    ++ V
Sbjct: 233 RNQLMSNDFEENISNILNFVQDTTMSDFHKLLPVWLIWRIWKARNNVVFNKFRESPSKTV 292

Query: 182 KRRIATSIDERPNLQEDNQNISELKSLSSH-LHWEPPDPGCWKLNADASWS-EKDEIGGI 241
               A + D     Q   +  S  + ++ + + W  P     K N DA +  +K E  G 
Sbjct: 293 LSAKAETHDWLNATQSHKKTPSPTRQIAENKIEWRNPPATYVKCNFDAGFDVQKLEATG- 352

Query: 242 GWAIRDSNGSLIGLGCKKISKLWNIKCLEAKAIIEGLKAYEDSGGFEGSRRKPPLVVESD 301
           GW IR+  G+ I  G  K++   N    E KA++  L+           R    + +E D
Sbjct: 353 GWIIRNHYGTPISWGSMKLAHTSNPLEAETKALLAALQQ-------TWIRGYTQVFMEGD 412

Query: 302 SVEVVNAVNRVADDATELCLFVDEIDSFRRSNRVRSFSKCSRQSNSLAHELAR 350
              ++N +N ++  ++ L   +++I  +        F    R+ N LAH LA+
Sbjct: 413 CQTLINLINGISFHSS-LANHLEDISFWANKFASIQFGFIRRKGNKLAHVLAK 456

BLAST of Spg025332 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 1.2e-09
Identity = 54/208 (25.96%), Postives = 91/208 (43.75%), Query Frame = 0

Query: 155 LLWHIWDARNTSNINNYLPDINRVKRRIATSIDERPN---------LQEDNQNISELKSL 214
           L+W IW + N    N+        + +  T+++   N         +  + QN +     
Sbjct: 47  LMWRIWKSGNDLVFNH-------TRTKFQTTVEMALNDTKEWLDNTMTNEQQNGNRNADP 106

Query: 215 SSHLHWEPPDPGCWKLNADASWSEKDEIGGIGWAIRDSNGSLIGLGCKKISKLWNIKCLE 274
           S +  W PP     K N DAS  E++ + G+GW +R+S G++I  G  K       +  E
Sbjct: 107 SRNTKWSPPGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKFQGRMTTEEAE 166

Query: 275 AKAIIEGLKAYEDSGGFEGSRRKPPLVVESDSVEVVNAVNRVADDATELCLFVDEIDSFR 334
              +I  ++A   S GF G ++   ++ E D+  +   +N  + +   L  F+D I S+ 
Sbjct: 167 CSTLIWAIQA---SYGF-GHKK---VIFEGDNQTITRMINTKSSN-PRLQHFLDTIQSWI 226

Query: 335 RSNRVRSFSKCSRQSNSLAHELARAAAK 354
            S     FS   R+ N  A  LA+ A K
Sbjct: 227 PSFESIEFSFKHREQNGCADFLAKQAIK 239

BLAST of Spg025332 vs. TAIR 10
Match: AT5G18880.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 53.9 bits (128), Expect = 3.4e-07
Identity = 45/168 (26.79%), Postives = 65/168 (38.69%), Query Frame = 0

Query: 2   SGSKDEIIWKYEDKGSFSVKSAYHLAKSLSSKDIASGSSDASSKSIW-KSIWKANCIPIA 61
           S  +D  +W+          +A     S SS+D        S    W K +W    IP  
Sbjct: 90  SRGQDSFLWR---------NAAGSYLPSFSSRDTWEQIRVHSPTVPWAKVVWFKEYIPRF 149

Query: 62  KITVWKILNDALPTKTNIAKKGIHTNLCCCLCRNGKESTAHIFWICKFSKKLWADFIPNA 121
            +  W    + LPT+  +   G++      LC NG E+ AH+F+ C FS  +W  F    
Sbjct: 150 SLITWMSFLERLPTRDRLRGWGMNIPSSWVLCSNGDETHAHLFFECSFSLAIWEFFASKF 209

Query: 122 SPLWLRCRDWEKPIDFWNFIQRNLSKEETRIAILLL----WHIWDARN 165
            P       +  P      +Q  L    T I  LLL    +H+W  RN
Sbjct: 210 RP----SPPFGLPAASSWILQLPLRSHSTTILKLLLQSAVYHVWKERN 244

BLAST of Spg025332 vs. TAIR 10
Match: AT1G52990.1 (thioredoxin family protein )

HSP 1 Score: 48.9 bits (115), Expect = 1.1e-05
Identity = 40/140 (28.57%), Postives = 62/140 (44.29%), Query Frame = 0

Query: 216 PGC-WKLNADASWSEKDEIGGIGWAIRDSNGSLIGLGCKKISKLWNIKCLEAKAIIEGLK 275
           P C  K N DAS  E D + G+GW IR+S G+++  G  K       +  E  A+I  ++
Sbjct: 44  PSCRVKCNYDASHHEGDVVSGLGWLIRNSQGTVLECGMGKFQGRMTPEEAECSALIWAIQ 103

Query: 276 AYEDSGGFEGSRRKPPLVVESDSVEVVNAVNRVADDATELCLFVDEIDSFRRSNRVRSFS 335
           A    G          ++ E D+  V   +N  +D+   L  ++D I S+  S     F 
Sbjct: 104 ATSAFG-------YTKVIFEGDNSNVNRLINTKSDN-PRLKHYLDTIKSWIPSFTSTEFI 163

Query: 336 KCSRQSNSLAHELARAAAKN 355
              R+ N  A  L + A K+
Sbjct: 164 FTHREQNQCADTLVKKAIKS 175

BLAST of Spg025332 vs. TAIR 10
Match: AT4G09775.1 (BEST Arabidopsis thaliana protein match is: Ribonuclease H-like superfamily protein (TAIR:AT2G02650.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 47.8 bits (112), Expect = 2.4e-05
Identity = 33/131 (25.19%), Postives = 53/131 (40.46%), Query Frame = 0

Query: 156 LWHIWDARNTSNINNYLPDINRVKRRIA------------TSIDERPNLQEDNQNISELK 215
           +W +W +RN          I+R   ++A            T+I++  N     Q      
Sbjct: 1   MWRLWKSRN----EFLFQQIDRFPWKVAQKGEQEATEWVETTINDTANSHSTEQPNDRPS 60

Query: 216 SLSSHLHWEPPDPGCWKLNADASWSEKDEIGGIGWAIRDSNGSLIGLGCKKISKLWNIKC 275
             S    W PP  G  K N D+ + +  +     W IRDSNG +I  GC K+ + ++   
Sbjct: 61  GRSK--EWSPPPEGYLKCNFDSGYVQGRDYTSTCWIIRDSNGHVIHSGCAKLQQSYSALQ 120