Spg025219 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg025219
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionNADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
Locationscaffold13: 40287236 .. 40291886 (+)
RNA-Seq ExpressionSpg025219
SyntenySpg025219
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGCCGGCAAAGGCGTCTTTGAGCAGATGTTTGTCGGTTTCCGAGCTCGGAATAGCATTCCATTCCGCTCGTATTCTTTTGTTGGAAGAACCTGAACTCTACCCGGTTCTGCACGAGTAGCAACAATTTAACTAGTATAATTTAAATTACTTCTTTGATTATTCCATATTAGTTGTTGCCTCCAAATGCCATGCAAAATGGCAAAATCACTGTGGAGCCAAGTTTCTTTGTCGAATGTTCTTGAAATTGTAGACGTGGCTGGCGTGGGCGCGCCCTATTCGTTGAAAAATCTCCAGCAGGGCAAAGGACTAGTTAGAAGCATCGCCTTTCTGGAAGCTTCGCGTTTTCTTGCGATTGATCGGGACTGTTATAAGCCCATCGGCGAGGAAAATGCGATTGAAAAGCAGTAATTACTCTGGGGATAGAGGAATCTGGTGGTCGTCGAGGCTAAAAGATGGCGAGCTCTGTGCTACGTAGGTACAATCTTGTTCGTTGGAATCGATCAGCTTTTGTTCATTGGAGAAGACTGCTTTCCTCGAGTTCCGACTCCGTGGTGGAGTTCAAGCCAGGGGAGATCGGCATAGTCTCCGGTATCCCTGAAGAGAATCTCCGTCGTCGGGTATGCATTTTAAGCGGTTGCTCTCCGTTTTTTGCTCTTCGTTTAGTTTAGTTTCGGATAGGTTAAAAATGTCTTGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACAACATGAAAAAAGTCTTGAAACTTTAAACTAACAATTTAGTCCTCAAATATATATTTATTTATTTATTTTTTTTTTTTTTAAAGTTTAGCAACCTTTGAACTTAAAAATATTTACTTTTAAGCTAGGTTCATTGTCGGTTTGAACTCCATAGTTTATAAACATATTTGTTCTCTAATTAATTTTTGGTTAAATGTTAAGTTTAATCATTGAACTTTCACATTGGTGTCTTTAGTTTTTAAACTTTAAAAAATGTCTTTATGCATAATAGATTTCTAAACCTATTTGAAATTTTTAAAAATTAATTGATTTATTAGATATGAAATTGAATTGGTTAAATTACAAGTTTAGTCCCTAAACTTTCAAGGTTGTATCAATTTGGTCCCTGAACTTTAAAAAGTGTTTAATAGGTTCTTGAACTTGTAATTTTGTGTCTAATAGGTCCCTAAACTTTAAAAATGTCTAATAGATCTTTGAATTTATAATTTTATATCTAATAGGTCTCTAACATGTTCAAGATTTTTAAAATTAATTGATCTATTAGATATAAAATAGAATTTTATGCCTATTGGGACTCTAAACTTCCAATTTTGTATCTAGTAGACTTGTTAATTTAAAAAAAAAAATGTTGAATTGATCAAGGACTTATTAGACATAAAGTTGAGAGTTGAGGAACCTATTGGACCCAAAAGTGAAAGTTTAAAGACTTACTGAATACTTTTTAAAGTGTAGGGACTTACTAGACACAAAATTGAAATTACAAGGATTCATTTGACACTTTTTAAAGTTCAAAGAACCATATTGAACTTTCAAAGTTCAAGGACTAAATTTGTAATTGAACCAATTGAATTTTATGTCTAATGGAATCCTAAACCTTCGATTTTATGTCTAATAGATTCGTGGATTTTAAAAAACCTTGAATAGATGAAGGATTTATCACATATAAAATTAATAATTCAAGAAACTATTGGACACTTCTTTAAAAAAAAAATAATAATAACTATTATTGGACACAAAATTGAAGGTTAAAGAACTTTGTTTGAATCAAATAAACACAAATTTGAAAGTTTAAGATCGTGATACTTTTTAAATTTAGAGCAAAAGACCAACCTGAAAATTGAAGTCTAAACTAAATCATTTGTAAAAATTCTTAAAATTGTTTCTTAAAGGAATGGAAGATTTAGACTAAATTTTTGTAGTTTAGAAATGTAAGTGTTTCTTAACCGTTTACTTAATCTTGAAGTTCTGTTGTATAGTTGATTTTCAAAGAATCTTGGAAAGAGAGTCAAATATAACCGTCAGATTCGAGATATGAACTCGAATCAGATTTAGCTCCATTTAGGAATTAGAGCGGAAATTTTTACTTTGTAAGGAAAACTTTGCTGCATGAGGGTTTATCATGGTGGATTTGTGGAATTACCATGCATTTGAGCTTATGGAGGAACTGATTGGATTGTCTTCCTTTATTTTGCAGGTTATAATCTACTCACCTGCTAGAACCGCCACACAGCAAGGCTCTGGGAAAGTTGGGAAATGGAAGATTAATTTTCTCTCCACACACAAGTATGATTTCTTCAATTGCAAAGCCCGGCCTGACTCCTCTGTTCTCTTGATCCTTTTCCTATATCAATTGATGCATATTTTATCTTATATCATACCTCATAGTTTACATACGATTTATGACATTTATTCACCATTTCTCATTACGGCAAGTCATTCCTCTAGCCTCGGACTAGAGAAAAAGAACTGAGATGAAATTCTGGATATTCAAAAACTGTCAAGTCCATGTAAAAATTGATTCTCTTGGCATTTATCCAGCTTTGGATTCACTCCATTGGATTTCGGTTTTGTTCTCATGCATTTTCAAGATATTTAGAACTGAAAGCAATTATTAATTAGCAAGCGTGCTGACTACTGGATTGGATTCCATTTTCTTTAGTTCATAGCTATTTTAGGTATCGTGAACTGATCGCCCTTTCTTATCTGGCTCCATTTGTTTAGGTGGGAAAACCCCTTGATGGGATGGACATCCACAGGAGACCCATATGCTAATGTCGGCGATGCAGCCCTTAGTTTTGATACTGCAGAAGCTGCAATGACGTTTGCTGAAAAGCATGGCTGGCAATACGAGGTTTCACTGAAATTTTGTTATCATTTACAATTGATACAGTTTCTGGGGCTTCCTTGCTCTTAAAATTGAAACAGAAACAATTTATGTTCTGTAATCAGAGTCGCTAACTTCTATTGTAGGTTAAGAAGCGTCAGACACCAATTCTTAAGGTAAAGTTATTGTTGATTAATAACCGAAACTACTCTTTCATGCTGCCCCCGTTAGTGTTTTCAGCATATACTTGACCAAAAAAGCTTCAGGTTCTTTCTTCAACAGCCAAATTGTTTTTATCACTAATTCTTATGAGTTGGAGAAGAATTTAGAAATCACCCTATGTGGTTTGTCTACTATTTTATACACAATTCCTATGCATTTTCCTGATGATAATCCACATTGATATAATTAAATTTGTCGTAACTTTCACTTTTAGGTTCAGAGATGATTCAACATGATATCAGAATAAGATGTCAGGTATTCGAACTCCTGTAAAGTCATTTTCTCTTCAATTAATATTGATTTCCACCTATTGAGCTTTCTACAAATTTTCAAGTCCACAAGTGACTTTTGAGTTCAATGGTGATTTAACACCAAGCACCTCTAGAGATAATACCATTCCATTAAAAACTTAGATTTATTGCAAGTTTAGTCTTTGAACTTTCAAGGTCGTATTAGGTCCTTGGACCCTGACATATTCGAAAATTTTAAAAATTAGGTCCCTGAATTTTCAATTTTGTGTGTAATAGGTCCCTGACATATTTGAATTAAAATTTTAAAAATTAACATATCTACTATATATAAAATTGAATTTTATGTCCATTGAGACTCTAAACTTTCAATTTTGTATCTAGTATGCTTATAAATTTTTAAAAATGTCGAATAGATCAATGACTTATTAGATACAAAATAAAAAAATTGAGGGACCTGTTAGACACAAAAGTGAAAGTTTAAGAACCTACCAGATACTCTTTAAAGTTCAAAGATCTATTGGAAACAAAATGGAAAGTCCGAAGACCTATTTGAAACTTTTTAAAGTTCATGGTTCATGTATTAAATTGACACAACCTGCAAAAATCCGGTACATTGCTTCCTAGCTTTTAAACTATTAGTGTGTATTAGTGTTAATTAATTATAAATTAGTTATATTGTTATATCCGTTGTACATTGTACTTACACAGGATGATAGTTTAAGTTTCTTAAGCTCATCAGTTCATCAATACAAGGGGCTGATCATCAGAGAGTTCTATCTATCATTACATTTCATTCTGAATTGTTCATAAAACACCTAATTTATTACTAAACTTTCCTTACATGAACTGTGAAGGTTTGTACAATCCTTCCAATTTTCTCTAACTTCATAATGACTTGGATGGATTTCCTCGAGAACTGCAGGCTACAAATTTCTTCTTTTTTCCATTGTTATTTTCATTTCAGGTTTGTTGTTTTTAATCTTGTGCAGCCGAAGTCGTATGCGGACAACTTCAAATTGAAAGGACCACCGCCGGAAGCTGAGGAAAATTAATTTGAAGCTTCAAGCTCCCTACCATGGAGCTGCCATGGGAACGTGAACCGTGAGAATCTGAAAAAAGCATATTTAGTTGCAATGTGAAAAGAAAACTGTATTTGATTGTGAATTTAAAATTTGTTAATGTGCGTTTGATAAAATCTTATTTTAATTTTAAGAAAAAAAAAAAACTAATTTGTGATGTAGAAAAAGATACTTGTTGCAACAAGAGGAAGAAAGATCGAGCATAGTTTAACGGTAATTGGCATGTATCCTCGACCAAGAAGTCTGAGGTTCGAATCTCCC

mRNA sequence

ATGCCGCCGGCAAAGGCGTCTTTGAGCAGATGTTTGTCGGTTTCCGAGCTCGGAATAGCATTCCATTCCGCTCGTATTCTTTTGTTGGAAGAACCTGAACTCTACCCGGTTCTGCACGATTGTTGCCTCCAAATGCCATGCAAAATGGCAAAATCACTGTGGAGCCAAGTTTCTTTGTCGAATGTTCTTGAAATTGTAGACGTGGCTGGCGTGGGCGCGCCCTATTCGTTGAAAAATCTCCAGCAGGGCAAAGGACTAGTTAGAAGCATCGCCTTTCTGGAAGCTTCGCGTTTTCTTGCGATTGATCGGGACTGTTATAAGCCCATCGGCGAGGAAAATGCGATTGAAAAGCAGTACAATCTTGTTCGTTGGAATCGATCAGCTTTTGTTCATTGGAGAAGACTGCTTTCCTCGAGTTCCGACTCCGTGGTGGAGTTCAAGCCAGGGGAGATCGGCATAGTCTCCGGTATCCCTGAAGAGAATCTCCGTCGTCGGGTTATAATCTACTCACCTGCTAGAACCGCCACACAGCAAGGCTCTGGGAAAGTTGGGAAATGGAAGATTAATTTTCTCTCCACACACAAGTGGGAAAACCCCTTGATGGGATGGACATCCACAGGAGACCCATATGCTAATGTCGGCGATGCAGCCCTTAGTTTTGATACTGCAGAAGCTGCAATGACGTTTGCTGAAAAGCATGGCTGGCAATACGAGGTTAAGAAGCGTCAGACACCAATTCTTAAGCCGAAGTCGTATGCGGACAACTTCAAATTGAAAGGACCACCGCCGGAAGCTGAGGAAAATTAA

Coding sequence (CDS)

ATGCCGCCGGCAAAGGCGTCTTTGAGCAGATGTTTGTCGGTTTCCGAGCTCGGAATAGCATTCCATTCCGCTCGTATTCTTTTGTTGGAAGAACCTGAACTCTACCCGGTTCTGCACGATTGTTGCCTCCAAATGCCATGCAAAATGGCAAAATCACTGTGGAGCCAAGTTTCTTTGTCGAATGTTCTTGAAATTGTAGACGTGGCTGGCGTGGGCGCGCCCTATTCGTTGAAAAATCTCCAGCAGGGCAAAGGACTAGTTAGAAGCATCGCCTTTCTGGAAGCTTCGCGTTTTCTTGCGATTGATCGGGACTGTTATAAGCCCATCGGCGAGGAAAATGCGATTGAAAAGCAGTACAATCTTGTTCGTTGGAATCGATCAGCTTTTGTTCATTGGAGAAGACTGCTTTCCTCGAGTTCCGACTCCGTGGTGGAGTTCAAGCCAGGGGAGATCGGCATAGTCTCCGGTATCCCTGAAGAGAATCTCCGTCGTCGGGTTATAATCTACTCACCTGCTAGAACCGCCACACAGCAAGGCTCTGGGAAAGTTGGGAAATGGAAGATTAATTTTCTCTCCACACACAAGTGGGAAAACCCCTTGATGGGATGGACATCCACAGGAGACCCATATGCTAATGTCGGCGATGCAGCCCTTAGTTTTGATACTGCAGAAGCTGCAATGACGTTTGCTGAAAAGCATGGCTGGCAATACGAGGTTAAGAAGCGTCAGACACCAATTCTTAAGCCGAAGTCGTATGCGGACAACTTCAAATTGAAAGGACCACCGCCGGAAGCTGAGGAAAATTAA

Protein sequence

MPPAKASLSRCLSVSELGIAFHSARILLLEEPELYPVLHDCCLQMPCKMAKSLWSQVSLSNVLEIVDVAGVGAPYSLKNLQQGKGLVRSIAFLEASRFLAIDRDCYKPIGEENAIEKQYNLVRWNRSAFVHWRRLLSSSSDSVVEFKPGEIGIVSGIPEENLRRRVIIYSPARTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFDTAEAAMTFAEKHGWQYEVKKRQTPILKPKSYADNFKLKGPPPEAEEN
Homology
BLAST of Spg025219 vs. NCBI nr
Match: XP_022131424.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Momordica charantia])

HSP 1 Score: 275.0 bits (702), Expect = 6.8e-70
Identity = 132/154 (85.71%), Postives = 141/154 (91.56%), Query Frame = 0

Query: 113 NAIEKQYNLVRWNRSAFVHWRRLLSSSSDSVVEFKPGEIGIVSGIPEENLRRRVIIYSPA 172
           +++ + YNL R +RSAFVHWRRLL  SSDS+VEFKP EIGIVSGIPE +LRRR+IIYSPA
Sbjct: 3   SSLLRSYNLARSDRSAFVHWRRLL--SSDSLVEFKPAEIGIVSGIPENHLRRRIIIYSPA 62

Query: 173 RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFDTAEAAMTFAEK 232
           RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFD+AEAAM FAEK
Sbjct: 63  RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFDSAEAAMAFAEK 122

Query: 233 HGWQYEVKKRQTPILKPKSYADNFKLKGPPPEAE 267
           HGWQYEVKKRQTPILKPKSYADNFK KGPP   E
Sbjct: 123 HGWQYEVKKRQTPILKPKSYADNFKWKGPPKTEE 154

BLAST of Spg025219 vs. NCBI nr
Match: XP_038884729.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 268.1 bits (684), Expect = 8.3e-68
Identity = 133/156 (85.26%), Postives = 142/156 (91.03%), Query Frame = 0

Query: 113 NAIEKQYNLVRWNRSAFVHWRRLLSSSSDSVVEFKPGEIGIVSGIPEENLRRRVIIYSPA 172
           N++  +YN  R   SAFVH  RLL  SSDS+VEFKPGEIG+VSGIPEE++RRRVIIYSPA
Sbjct: 3   NSLLGRYNFDRSKLSAFVHCTRLL--SSDSLVEFKPGEIGMVSGIPEEHIRRRVIIYSPA 62

Query: 173 RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFDTAEAAMTFAEK 232
           RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFD+AEAAM FAEK
Sbjct: 63  RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFDSAEAAMRFAEK 122

Query: 233 HGWQYEVKKRQTPILKPKSYADNFKLKGPPPEAEEN 269
           HGWQYEVKKRQTPILKPKSYADNFK KG PP+AEEN
Sbjct: 123 HGWQYEVKKRQTPILKPKSYADNFKWKG-PPKAEEN 155

BLAST of Spg025219 vs. NCBI nr
Match: XP_008444538.1 (PREDICTED: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucumis melo])

HSP 1 Score: 263.1 bits (671), Expect = 2.7e-66
Identity = 128/156 (82.05%), Postives = 141/156 (90.38%), Query Frame = 0

Query: 113 NAIEKQYNLVRWNRSAFVHWRRLLSSSSDSVVEFKPGEIGIVSGIPEENLRRRVIIYSPA 172
           N++ ++YN  R   SAFVH  RLL  SSDS+VEFKPGE+G+ SGIPEE+L RRVIIYSPA
Sbjct: 3   NSLLRRYNFDRSKLSAFVHCTRLL--SSDSLVEFKPGEVGLASGIPEEHLNRRVIIYSPA 62

Query: 173 RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFDTAEAAMTFAEK 232
           RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVG+A+L+FD+AEAAM FAEK
Sbjct: 63  RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGEASLTFDSAEAAMRFAEK 122

Query: 233 HGWQYEVKKRQTPILKPKSYADNFKLKGPPPEAEEN 269
           HGWQYEVKKRQTPILKPKSYADNFK KG PP+AEEN
Sbjct: 123 HGWQYEVKKRQTPILKPKSYADNFKWKG-PPKAEEN 155

BLAST of Spg025219 vs. NCBI nr
Match: KAA0060960.1 (NADH dehydrogenase (ubiquinone) iron-sulfur protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 262.7 bits (670), Expect = 3.5e-66
Identity = 128/156 (82.05%), Postives = 141/156 (90.38%), Query Frame = 0

Query: 113 NAIEKQYNLVRWNRSAFVHWRRLLSSSSDSVVEFKPGEIGIVSGIPEENLRRRVIIYSPA 172
           N++ ++YN  R   SAFVH  RLL  SSDS+VEFKPGEIG+ SGIPEE+L RRVIIYSPA
Sbjct: 3   NSLLRRYNFDRSKLSAFVHCTRLL--SSDSLVEFKPGEIGLASGIPEEHLNRRVIIYSPA 62

Query: 173 RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFDTAEAAMTFAEK 232
           RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVG+A+L+FD+AEAAM FAEK
Sbjct: 63  RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGEASLTFDSAEAAMRFAEK 122

Query: 233 HGWQYEVKKRQTPILKPKSYADNFKLKGPPPEAEEN 269
           HGWQYEVKKRQTP+LKPKSYADNFK KG PP+AEEN
Sbjct: 123 HGWQYEVKKRQTPLLKPKSYADNFKWKG-PPKAEEN 155

BLAST of Spg025219 vs. NCBI nr
Match: XP_004142898.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Cucumis sativus] >KGN62438.1 hypothetical protein Csa_018679 [Cucumis sativus])

HSP 1 Score: 262.3 bits (669), Expect = 4.5e-66
Identity = 131/156 (83.97%), Postives = 137/156 (87.82%), Query Frame = 0

Query: 113 NAIEKQYNLVRWNRSAFVHWRRLLSSSSDSVVEFKPGEIGIVSGIPEENLRRRVIIYSPA 172
           N +  +YN  R   SAFVH  RLL  SSDS+VE KPGEIGI SGIPEE+L RRVIIYSPA
Sbjct: 3   NPLLHRYNFDRSRLSAFVHCTRLL--SSDSLVEVKPGEIGIASGIPEEHLHRRVIIYSPA 62

Query: 173 RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFDTAEAAMTFAEK 232
           RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFD+AEAAM FAEK
Sbjct: 63  RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFDSAEAAMRFAEK 122

Query: 233 HGWQYEVKKRQTPILKPKSYADNFKLKGPPPEAEEN 269
           HGWQYEVKKRQTP+LKPKSYADNFK KG PP AEEN
Sbjct: 123 HGWQYEVKKRQTPLLKPKSYADNFKWKG-PPRAEEN 155

BLAST of Spg025219 vs. ExPASy Swiss-Prot
Match: Q9FJW4 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO1 PE=1 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.1e-50
Identity = 97/129 (75.19%), Postives = 113/129 (87.60%), Query Frame = 0

Query: 139 SSDSVVE--FKPGEIGIVSGIPEENLRRRVIIYSPARTATQQGSGKVGKWKINFLSTHKW 198
           ++D+VVE  +K GEIG VSGIPEE+L R+VIIYSPARTATQ GSGK+GKWKINF+ST KW
Sbjct: 25  ATDAVVESDYKRGEIGKVSGIPEEHLSRKVIIYSPARTATQSGSGKLGKWKINFVSTLKW 84

Query: 199 ENPLMGWTSTGDPYANVGDAALSFDTAEAAMTFAEKHGWQYEVKKRQTPILKPKSYADNF 258
           ENPLMGWTSTGDPYANVGD+AL+FD+ EAA +FAE+HGW Y+VKK  TP+LK KSY+DNF
Sbjct: 85  ENPLMGWTSTGDPYANVGDSALAFDSEEAAKSFAERHGWDYKVKKPNTPLLKVKSYSDNF 144

Query: 259 KLKG-PPPE 265
           K KG P PE
Sbjct: 145 KWKGNPQPE 153

BLAST of Spg025219 vs. ExPASy Swiss-Prot
Match: Q0MQH0 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gorilla gorilla gorilla OX=9595 GN=NDUFS4 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.4e-22
Identity = 50/108 (46.30%), Postives = 73/108 (67.59%), Query Frame = 0

Query: 150 EIGIVSGIPEENLR-RRVIIYSPARTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGD 209
           +I  ++G+PEE+++ R+V I+ PAR   Q G     KWK+ F +  +WENPLMGW ST D
Sbjct: 60  DITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTAD 119

Query: 210 PYANVGDAALSFDTAEAAMTFAEKHGWQYEVKKRQTPILKPKSYADNF 257
           P +N+    L+F T E A++FAEK+GW Y++++R+ P  K KSY  NF
Sbjct: 120 PLSNM---VLTFSTKEDAVSFAEKNGWSYDIEERKVPKPKSKSYGANF 164

BLAST of Spg025219 vs. ExPASy Swiss-Prot
Match: O43181 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Homo sapiens OX=9606 GN=NDUFS4 PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.4e-22
Identity = 50/108 (46.30%), Postives = 73/108 (67.59%), Query Frame = 0

Query: 150 EIGIVSGIPEENLR-RRVIIYSPARTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGD 209
           +I  ++G+PEE+++ R+V I+ PAR   Q G     KWK+ F +  +WENPLMGW ST D
Sbjct: 60  DITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTAD 119

Query: 210 PYANVGDAALSFDTAEAAMTFAEKHGWQYEVKKRQTPILKPKSYADNF 257
           P +N+    L+F T E A++FAEK+GW Y++++R+ P  K KSY  NF
Sbjct: 120 PLSNM---VLTFSTKEDAVSFAEKNGWSYDIEERKVPKPKSKSYGANF 164

BLAST of Spg025219 vs. ExPASy Swiss-Prot
Match: P0CB95 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Pongo abelii OX=9601 GN=NDUFS4 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.9e-22
Identity = 51/108 (47.22%), Postives = 73/108 (67.59%), Query Frame = 0

Query: 150 EIGIVSGIPEENLR-RRVIIYSPARTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGD 209
           +I  ++G+PEE+++ R+V I+ PAR   Q G     KWK+ F +  +WENPLMGW ST D
Sbjct: 60  DITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTAD 119

Query: 210 PYANVGDAALSFDTAEAAMTFAEKHGWQYEVKKRQTPILKPKSYADNF 257
           P +N+    L+F T E A++FAEK+GW Y+V++R+ P  K KSY  NF
Sbjct: 120 PLSNM---VLTFRTKEDAVSFAEKNGWSYDVEERKVPKPKSKSYGANF 164

BLAST of Spg025219 vs. ExPASy Swiss-Prot
Match: P0CB96 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Pongo pygmaeus OX=9600 GN=NDUFS4 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.9e-22
Identity = 51/108 (47.22%), Postives = 73/108 (67.59%), Query Frame = 0

Query: 150 EIGIVSGIPEENLR-RRVIIYSPARTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGD 209
           +I  ++G+PEE+++ R+V I+ PAR   Q G     KWK+ F +  +WENPLMGW ST D
Sbjct: 60  DITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTAD 119

Query: 210 PYANVGDAALSFDTAEAAMTFAEKHGWQYEVKKRQTPILKPKSYADNF 257
           P +N+    L+F T E A++FAEK+GW Y+V++R+ P  K KSY  NF
Sbjct: 120 PLSNM---VLTFRTKEDAVSFAEKNGWSYDVEERKVPKPKSKSYGANF 164

BLAST of Spg025219 vs. ExPASy TrEMBL
Match: A0A6J1BQ75 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111004639 PE=3 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 3.3e-70
Identity = 132/154 (85.71%), Postives = 141/154 (91.56%), Query Frame = 0

Query: 113 NAIEKQYNLVRWNRSAFVHWRRLLSSSSDSVVEFKPGEIGIVSGIPEENLRRRVIIYSPA 172
           +++ + YNL R +RSAFVHWRRLL  SSDS+VEFKP EIGIVSGIPE +LRRR+IIYSPA
Sbjct: 3   SSLLRSYNLARSDRSAFVHWRRLL--SSDSLVEFKPAEIGIVSGIPENHLRRRIIIYSPA 62

Query: 173 RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFDTAEAAMTFAEK 232
           RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFD+AEAAM FAEK
Sbjct: 63  RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFDSAEAAMAFAEK 122

Query: 233 HGWQYEVKKRQTPILKPKSYADNFKLKGPPPEAE 267
           HGWQYEVKKRQTPILKPKSYADNFK KGPP   E
Sbjct: 123 HGWQYEVKKRQTPILKPKSYADNFKWKGPPKTEE 154

BLAST of Spg025219 vs. ExPASy TrEMBL
Match: A0A1S3BAL3 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103487828 PE=3 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 1.3e-66
Identity = 128/156 (82.05%), Postives = 141/156 (90.38%), Query Frame = 0

Query: 113 NAIEKQYNLVRWNRSAFVHWRRLLSSSSDSVVEFKPGEIGIVSGIPEENLRRRVIIYSPA 172
           N++ ++YN  R   SAFVH  RLL  SSDS+VEFKPGE+G+ SGIPEE+L RRVIIYSPA
Sbjct: 3   NSLLRRYNFDRSKLSAFVHCTRLL--SSDSLVEFKPGEVGLASGIPEEHLNRRVIIYSPA 62

Query: 173 RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFDTAEAAMTFAEK 232
           RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVG+A+L+FD+AEAAM FAEK
Sbjct: 63  RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGEASLTFDSAEAAMRFAEK 122

Query: 233 HGWQYEVKKRQTPILKPKSYADNFKLKGPPPEAEEN 269
           HGWQYEVKKRQTPILKPKSYADNFK KG PP+AEEN
Sbjct: 123 HGWQYEVKKRQTPILKPKSYADNFKWKG-PPKAEEN 155

BLAST of Spg025219 vs. ExPASy TrEMBL
Match: A0A5A7V0V4 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00940 PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.7e-66
Identity = 128/156 (82.05%), Postives = 141/156 (90.38%), Query Frame = 0

Query: 113 NAIEKQYNLVRWNRSAFVHWRRLLSSSSDSVVEFKPGEIGIVSGIPEENLRRRVIIYSPA 172
           N++ ++YN  R   SAFVH  RLL  SSDS+VEFKPGEIG+ SGIPEE+L RRVIIYSPA
Sbjct: 3   NSLLRRYNFDRSKLSAFVHCTRLL--SSDSLVEFKPGEIGLASGIPEEHLNRRVIIYSPA 62

Query: 173 RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFDTAEAAMTFAEK 232
           RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVG+A+L+FD+AEAAM FAEK
Sbjct: 63  RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGEASLTFDSAEAAMRFAEK 122

Query: 233 HGWQYEVKKRQTPILKPKSYADNFKLKGPPPEAEEN 269
           HGWQYEVKKRQTP+LKPKSYADNFK KG PP+AEEN
Sbjct: 123 HGWQYEVKKRQTPLLKPKSYADNFKWKG-PPKAEEN 155

BLAST of Spg025219 vs. ExPASy TrEMBL
Match: A0A0A0LKZ2 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis sativus OX=3659 GN=Csa_2G354640 PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 2.2e-66
Identity = 131/156 (83.97%), Postives = 137/156 (87.82%), Query Frame = 0

Query: 113 NAIEKQYNLVRWNRSAFVHWRRLLSSSSDSVVEFKPGEIGIVSGIPEENLRRRVIIYSPA 172
           N +  +YN  R   SAFVH  RLL  SSDS+VE KPGEIGI SGIPEE+L RRVIIYSPA
Sbjct: 3   NPLLHRYNFDRSRLSAFVHCTRLL--SSDSLVEVKPGEIGIASGIPEEHLHRRVIIYSPA 62

Query: 173 RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFDTAEAAMTFAEK 232
           RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFD+AEAAM FAEK
Sbjct: 63  RTATQQGSGKVGKWKINFLSTHKWENPLMGWTSTGDPYANVGDAALSFDSAEAAMRFAEK 122

Query: 233 HGWQYEVKKRQTPILKPKSYADNFKLKGPPPEAEEN 269
           HGWQYEVKKRQTP+LKPKSYADNFK KG PP AEEN
Sbjct: 123 HGWQYEVKKRQTPLLKPKSYADNFKWKG-PPRAEEN 155

BLAST of Spg025219 vs. ExPASy TrEMBL
Match: A0A7J6EM59 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cannabis sativa OX=3483 GN=F8388_003349 PE=3 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 1.3e-58
Identity = 109/136 (80.15%), Postives = 122/136 (89.71%), Query Frame = 0

Query: 132 WRRLLSSSSDSVVEFKPGEIGIVSGIPEENLRRRVIIYSPARTATQQGSGKVGKWKINFL 191
           W+R    SSD++V+ KPGE+ ++SGIPEE+LRRRVIIYSPARTATQQGSGKVGKWKINFL
Sbjct: 20  WKRWF--SSDALVDLKPGEVAVISGIPEEHLRRRVIIYSPARTATQQGSGKVGKWKINFL 79

Query: 192 STHKWENPLMGWTSTGDPYANVGDAALSFDTAEAAMTFAEKHGWQYEVKKRQTPILKPKS 251
           STHKWENPLMGWTSTGDPYANVG+A L+FDT EAA  FAEK+GWQYEVKKR TP+LKPKS
Sbjct: 80  STHKWENPLMGWTSTGDPYANVGEAGLTFDTEEAAKAFAEKYGWQYEVKKRHTPLLKPKS 139

Query: 252 YADNFKLKGPPPEAEE 268
           YADNFK KGPPP+  E
Sbjct: 140 YADNFKWKGPPPQGVE 153

BLAST of Spg025219 vs. TAIR 10
Match: AT5G67590.1 (NADH-ubiquinone oxidoreductase-related )

HSP 1 Score: 200.7 bits (509), Expect = 1.5e-51
Identity = 97/129 (75.19%), Postives = 113/129 (87.60%), Query Frame = 0

Query: 139 SSDSVVE--FKPGEIGIVSGIPEENLRRRVIIYSPARTATQQGSGKVGKWKINFLSTHKW 198
           ++D+VVE  +K GEIG VSGIPEE+L R+VIIYSPARTATQ GSGK+GKWKINF+ST KW
Sbjct: 25  ATDAVVESDYKRGEIGKVSGIPEEHLSRKVIIYSPARTATQSGSGKLGKWKINFVSTLKW 84

Query: 199 ENPLMGWTSTGDPYANVGDAALSFDTAEAAMTFAEKHGWQYEVKKRQTPILKPKSYADNF 258
           ENPLMGWTSTGDPYANVGD+AL+FD+ EAA +FAE+HGW Y+VKK  TP+LK KSY+DNF
Sbjct: 85  ENPLMGWTSTGDPYANVGDSALAFDSEEAAKSFAERHGWDYKVKKPNTPLLKVKSYSDNF 144

Query: 259 KLKG-PPPE 265
           K KG P PE
Sbjct: 145 KWKGNPQPE 153

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131424.16.8e-7085.71NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Momor... [more]
XP_038884729.18.3e-6885.26NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Benin... [more]
XP_008444538.12.7e-6682.05PREDICTED: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-... [more]
KAA0060960.13.5e-6682.05NADH dehydrogenase (ubiquinone) iron-sulfur protein 4 [Cucumis melo var. makuwa][more]
XP_004142898.14.5e-6683.97NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Cucumis sa... [more]
Match NameE-valueIdentityDescription
Q9FJW42.1e-5075.19NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Arabidop... [more]
Q0MQH01.4e-2246.30NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gorilla ... [more]
O431811.4e-2246.30NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Homo sap... [more]
P0CB951.9e-2247.22NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Pongo ab... [more]
P0CB961.9e-2247.22NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Pongo py... [more]
Match NameE-valueIdentityDescription
A0A6J1BQ753.3e-7085.71NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Momordic... [more]
A0A1S3BAL31.3e-6682.05NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis ... [more]
A0A5A7V0V41.7e-6682.05NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis ... [more]
A0A0A0LKZ22.2e-6683.97NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis ... [more]
A0A7J6EM591.3e-5880.15NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cannabis... [more]
Match NameE-valueIdentityDescription
AT5G67590.11.5e-5175.19NADH-ubiquinone oxidoreductase-related [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038532NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamilyGENE3D3.30.160.190atu1810 like domaincoord: 164..239
e-value: 2.9E-34
score: 118.9
IPR006885NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrialPFAMPF04800ETC_C1_NDUFA4coord: 165..259
e-value: 6.3E-33
score: 112.9
IPR006885NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrialPANTHERPTHR12219NADH-UBIQUINONE OXIDOREDUCTASEcoord: 119..266
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 248..268
NoneNo IPR availablePANTHERPTHR12219:SF23NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 4, MITOCHONDRIAL-LIKEcoord: 119..266

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg025219.1Spg025219.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0022900 electron transport chain
cellular_component GO:0005743 mitochondrial inner membrane
cellular_component GO:0070469 respirasome
molecular_function GO:0016651 oxidoreductase activity, acting on NAD(P)H