Spg024787 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg024787
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectinesterase
Location	scaffold12: 9647648 .. 9649697 (-)
RNA-Seq Expression	Spg024787
Synteny	Spg024787

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGAACGCCGAAGGTGACAAAAAGAAGAAGAATATCGCCATAGTAAGCGTCTCTGCTCTGATCCTCGTTGCAATGGTCGTCGCCGTTACTGTTGGCGTCAACAGCTCACCCCACGGTGACGATCACGGTGAGGGTCATGGTGACGGTCACGGTGACGGAGGTGCTGAAATCAAGACATCGACGAAAGCAATCCAGTCCATTTGTCGACCAACGGATTTCAAAGAAACCTGCGAGCAGAGCCTGATGGCTGAAGCCAGTAACACGACGGACCCGAAAGAGCTCGCGAAAGCCGGCTTCAAAGCCGCCGTCGAATATATAAACACGGCGATTAGAAATTCCACCACACTCAAAGAGCTGGCCAAGGAACCAAGAACCAACCAAGCCCTTCAGAACTGCCGCGAGCTTCTGGAATACGCCATCGACGATTTAACCCAATCTTTCAACAGAATCGGCACCTTCGAAATGTCGAAACTCGACGAGTTCGTCAACGATTTGAAGATCTGGCTGAGTGGGGCCCTCACGTACGAGCAGACTTGTTTGGACGGATTCGAAAACACAACCGGTGATGCCGGTCAGAAAATGCAGCAGTTTTTGAAGTCCGCTCAGGAAATGACGAGTAACGGTCTTGCGATCGTAACTGAATTGTCCACTATTATGGGATCACTTCATCAAATGCCGGGAGCGACGACGGGACGACGGCTGTTGGACGAGGGAGTGCCGTCGTGGGTGAGCGAAGGAAAGAGGCGCTTGATGCAGGCGGGACCGGCGGGGCTGAAACCGGACGTGGTGGTGGCTCAGGATGGAAGTGGAAAATACAAGACGATCTCTGAAGCTTTGGTTGATGTGCCGAAGAAGAGTAATAAGACATTTGTGATTTATGTGAAGGCTGGAATTTATAAGGAAATCGTTGTTGTTCCTAAGGATGTGACTCATCTTACGATGTACGGCGATGGCCCAACCAAGACTGTCGTCACTGGTAGTCTCAACTTTATCGATGGCGTTCAAACATTCAAGACCGCAACTTTCTGTAAGTCCAATAATATAAATATATTTTTACTCTTTTATATATATATATATATGCTAAAGTCTAATGAATTCGATTTGATATTTAAACAGCTACGATCGGAAGTGATTTCTTCGCGAAAGACATGGGATTCGTGAACAGTGCTGGAGCTGCAAAGCACCAAGCCGTGGCGTTACGAGTCCAATCCGATAGAGCGATCTTTTACAACTGCCAAATGGATGGGTATCAAGATACTCTCTACGCCCATGCCCATCGCCAGTTCTACCGTGACTGTTCGATCAGTGGCACCATCGACTTCGTCTTCGGCAACGCCGCCGCCGTCTTCCAGAACTGCAAACTAATCGTCCGAAAGCCAATGGATAACCAACAGTGCATCGTCACGGCTCAAGGCCGGCTCAACCGCCGCGAACCGACCGCCCTAGTGTTCCAAAGCTGCCACTTCATGGGCGACCCGGCTTACTTGCCATTCAAGGCCGTCAACAAGGCTTACTTGGGGCGACCCTGGAAGGAATATTCACGAACCCTCATTATCGGGTCTCAAATCGACGATTTGATCCAGCCCGAGGGATGGTTGCCGTGGGGTGGCGATTTTGGGCTGAACACGCTGTTCTACGCGGAGGTTCAGAACAAGGGACCAGGTGCCGATCTGAGCAAGAGGGTGAAGTGGCGAGGAATTAAAACTATTACGCCAGATCACGCCGCTAAATTCACTCCCAGGGTATTTATCGACGGCGATGCTTGGATTAAAAACAAGGGAATTCCGTATGCTTCTGGAATGATGTAAAAAGGGAACCAAAGAGACAGCTTATTTCACACTTCGGCGTTATTAATGTAACCATTTCGACTCAATTGGAGGAAATAATTTGAATTACGATTAATAATTTCTTGCCCTTTTTCTTTCGTTTCATTGTTTGGATCAAAGATTTTGCTTGTATTTTTTGTGTATTTGTGTGGTTTCTCCACCATGCAAATACCCCACAAGAGTATATAAATAAAGGAAAAAAAGTTTAAATTAAGGTTTGG

mRNA sequence

ATGTCGAACGCCGAAGGTGACAAAAAGAAGAAGAATATCGCCATAGTAAGCGTCTCTGCTCTGATCCTCGTTGCAATGGTCGTCGCCGTTACTGTTGGCGTCAACAGCTCACCCCACGGTGACGATCACGGTGAGGGTCATGGTGACGGTCACGGTGACGGAGGTGCTGAAATCAAGACATCGACGAAAGCAATCCAGTCCATTTGTCGACCAACGGATTTCAAAGAAACCTGCGAGCAGAGCCTGATGGCTGAAGCCAGTAACACGACGGACCCGAAAGAGCTCGCGAAAGCCGGCTTCAAAGCCGCCGTCGAATATATAAACACGGCGATTAGAAATTCCACCACACTCAAAGAGCTGGCCAAGGAACCAAGAACCAACCAAGCCCTTCAGAACTGCCGCGAGCTTCTGGAATACGCCATCGACGATTTAACCCAATCTTTCAACAGAATCGGCACCTTCGAAATGTCGAAACTCGACGAGTTCGTCAACGATTTGAAGATCTGGCTGAGTGGGGCCCTCACGTACGAGCAGACTTGTTTGGACGGATTCGAAAACACAACCGGTGATGCCGGTCAGAAAATGCAGCAGTTTTTGAAGTCCGCTCAGGAAATGACGAGTAACGGTCTTGCGATCGTAACTGAATTGTCCACTATTATGGGATCACTTCATCAAATGCCGGGAGCGACGACGGGACGACGGCTGTTGGACGAGGGAGTGCCGTCGTGGGTGAGCGAAGGAAAGAGGCGCTTGATGCAGGCGGGACCGGCGGGGCTGAAACCGGACGTGGTGGTGGCTCAGGATGGAAGTGGAAAATACAAGACGATCTCTGAAGCTTTGGTTGATGTGCCGAAGAAGAGTAATAAGACATTTGTGATTTATGTGAAGGCTGGAATTTATAAGGAAATCGTTGTTGTTCCTAAGGATGTGACTCATCTTACGATGTACGGCGATGGCCCAACCAAGACTGTCGTCACTGGTAGTCTCAACTTTATCGATGGCGTTCAAACATTCAAGACCGCAACTTTCTCTACGATCGGAAGTGATTTCTTCGCGAAAGACATGGGATTCGTGAACAGTGCTGGAGCTGCAAAGCACCAAGCCGTGGCGTTACGAGTCCAATCCGATAGAGCGATCTTTTACAACTGCCAAATGGATGGGTATCAAGATACTCTCTACGCCCATGCCCATCGCCAGTTCTACCGTGACTGTTCGATCAGTGGCACCATCGACTTCGTCTTCGGCAACGCCGCCGCCGTCTTCCAGAACTGCAAACTAATCGTCCGAAAGCCAATGGATAACCAACAGTGCATCGTCACGGCTCAAGGCCGGCTCAACCGCCGCGAACCGACCGCCCTAGTGTTCCAAAGCTGCCACTTCATGGGCGACCCGGCTTACTTGCCATTCAAGGCCGTCAACAAGGCTTACTTGGGGCGACCCTGGAAGGAATATTCACGAACCCTCATTATCGGGTCTCAAATCGACGATTTGATCCAGCCCGAGGGATGGTTGCCGTGGGGTGGCGATTTTGGGCTGAACACGCTGTTCTACGCGGAGGTTCAGAACAAGGGACCAGGTGCCGATCTGAGCAAGAGGGTGAAGTGGCGAGGAATTAAAACTATTACGCCAGATCACGCCGCTAAATTCACTCCCAGGGTATTTATCGACGGCGATGCTTGGATTAAAAACAAGGGAATTCCGTATGCTTCTGGAATGATGTAA

Coding sequence (CDS)

ATGTCGAACGCCGAAGGTGACAAAAAGAAGAAGAATATCGCCATAGTAAGCGTCTCTGCTCTGATCCTCGTTGCAATGGTCGTCGCCGTTACTGTTGGCGTCAACAGCTCACCCCACGGTGACGATCACGGTGAGGGTCATGGTGACGGTCACGGTGACGGAGGTGCTGAAATCAAGACATCGACGAAAGCAATCCAGTCCATTTGTCGACCAACGGATTTCAAAGAAACCTGCGAGCAGAGCCTGATGGCTGAAGCCAGTAACACGACGGACCCGAAAGAGCTCGCGAAAGCCGGCTTCAAAGCCGCCGTCGAATATATAAACACGGCGATTAGAAATTCCACCACACTCAAAGAGCTGGCCAAGGAACCAAGAACCAACCAAGCCCTTCAGAACTGCCGCGAGCTTCTGGAATACGCCATCGACGATTTAACCCAATCTTTCAACAGAATCGGCACCTTCGAAATGTCGAAACTCGACGAGTTCGTCAACGATTTGAAGATCTGGCTGAGTGGGGCCCTCACGTACGAGCAGACTTGTTTGGACGGATTCGAAAACACAACCGGTGATGCCGGTCAGAAAATGCAGCAGTTTTTGAAGTCCGCTCAGGAAATGACGAGTAACGGTCTTGCGATCGTAACTGAATTGTCCACTATTATGGGATCACTTCATCAAATGCCGGGAGCGACGACGGGACGACGGCTGTTGGACGAGGGAGTGCCGTCGTGGGTGAGCGAAGGAAAGAGGCGCTTGATGCAGGCGGGACCGGCGGGGCTGAAACCGGACGTGGTGGTGGCTCAGGATGGAAGTGGAAAATACAAGACGATCTCTGAAGCTTTGGTTGATGTGCCGAAGAAGAGTAATAAGACATTTGTGATTTATGTGAAGGCTGGAATTTATAAGGAAATCGTTGTTGTTCCTAAGGATGTGACTCATCTTACGATGTACGGCGATGGCCCAACCAAGACTGTCGTCACTGGTAGTCTCAACTTTATCGATGGCGTTCAAACATTCAAGACCGCAACTTTCTCTACGATCGGAAGTGATTTCTTCGCGAAAGACATGGGATTCGTGAACAGTGCTGGAGCTGCAAAGCACCAAGCCGTGGCGTTACGAGTCCAATCCGATAGAGCGATCTTTTACAACTGCCAAATGGATGGGTATCAAGATACTCTCTACGCCCATGCCCATCGCCAGTTCTACCGTGACTGTTCGATCAGTGGCACCATCGACTTCGTCTTCGGCAACGCCGCCGCCGTCTTCCAGAACTGCAAACTAATCGTCCGAAAGCCAATGGATAACCAACAGTGCATCGTCACGGCTCAAGGCCGGCTCAACCGCCGCGAACCGACCGCCCTAGTGTTCCAAAGCTGCCACTTCATGGGCGACCCGGCTTACTTGCCATTCAAGGCCGTCAACAAGGCTTACTTGGGGCGACCCTGGAAGGAATATTCACGAACCCTCATTATCGGGTCTCAAATCGACGATTTGATCCAGCCCGAGGGATGGTTGCCGTGGGGTGGCGATTTTGGGCTGAACACGCTGTTCTACGCGGAGGTTCAGAACAAGGGACCAGGTGCCGATCTGAGCAAGAGGGTGAAGTGGCGAGGAATTAAAACTATTACGCCAGATCACGCCGCTAAATTCACTCCCAGGGTATTTATCGACGGCGATGCTTGGATTAAAAACAAGGGAATTCCGTATGCTTCTGGAATGATGTAA

Protein sequence

MSNAEGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHGDGGAEIKTSTKAIQSICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAIRNSTTLKELAKEPRTNQALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTCLDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGATTGRRLLDEGVPSWVSEGKRRLMQAGPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYVKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGVQTFKTATFSTIGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNKAYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKNKGIPYASGMM

Homology

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BLAST of Spg024787 vs. NCBI nr
Match: XP_004150503.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucumis sativus] >KGN47423.1 hypothetical protein Csa_021732 [Cucumis sativus])

HSP 1 Score: 950.7 bits (2456), Expect = 5.9e-273
Identity = 470/576 (81.60%), Postives = 522/576 (90.62%), Query Frame = 0


Query: 2   SNAEGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHG-DGGAEIKT 61
           +NAEGDKKKK IAI+SVS+LILVAMVVAVTVGVN++PHG++HG+   D HG +   EI +
Sbjct: 6   ANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGD---DNHGSETTPEITS 65

Query: 62  STKAIQSICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAIRNSTTLKEL 121
           STKAIQ+IC+PTD+KETCE+SL AEA NTTDPKEL K GFK A   +N AI+NSTTLKEL
Sbjct: 66  STKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLKEL 125

Query: 122 AKEPRTNQALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTC 181
           AK+PRTNQALQNCRELLEYAIDDL QSF RIGTF+MSKLD+FV DLKIWLSGALTYEQTC
Sbjct: 126 AKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQTC 185

Query: 182 LDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGATTGRRLL---D 241
           LDGFENTTGDAG +MQ+FLKSAQ+MT+NGL IV ELSTI+GSL Q+PG  +GRRLL   D
Sbjct: 186 LDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSL-QLPG-MSGRRLLEDDD 245

Query: 242 EGVPSWVSEGKRRLMQAGPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYVKA 301
             +PSWVS+GKRRLMQAG A +KPD+VVAQDGSGKYKTI+ AL DVP KSNKTFVI+VKA
Sbjct: 246 REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA 305

Query: 302 GIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGVQTFKTATFSTIGSDFFAKDMGF 361
           GIYKEIVV+PK +THLTMYGDGPTKTVVTGSLNFIDG+QTFKTATFS IG++F+A+DMGF
Sbjct: 306 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 365

Query: 362 VNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNA 421
            N+AGAAKHQAVALRVQSDR+IF+NCQ+DGYQDTLYAHAHRQFYRDC+ISGTIDFVFGNA
Sbjct: 366 ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 425

Query: 422 AAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNKAYL 481
           A  FQNCKL+VRKP+DNQQCIVTA GRLNR+EPTAL+FQSCHFMGDPAYLPFKA+NKAYL
Sbjct: 426 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL 485

Query: 482 GRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVKWRG 541
           GRPWKEYSRT+IIGS IDD+IQPEGWLPW GDFGLNTLFYAEVQNKG GAD SKRVKWRG
Sbjct: 486 GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG 545

Query: 542 IKTITPDHAAKFTPRVFIDGDAWIKNKGIPYASGMM 574
           IK ITP HAA FTPR FIDGDAWI  KGIPY+SGMM
Sbjct: 546 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

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BLAST of Spg024787 vs. NCBI nr
Match: XP_008458409.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo])

HSP 1 Score: 946.4 bits (2445), Expect = 1.1e-271
Identity = 466/575 (81.04%), Postives = 521/575 (90.61%), Query Frame = 0


Query: 2   SNAEGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHGDGGAEIKTS 61
           +NAEGDKKKK IAI+SVS+LILVAMVVAVTVG+N++P+ ++HGE  GD       EI +S
Sbjct: 6   ANAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPY-EEHGE--GDQGSKTTPEISSS 65

Query: 62  TKAIQSICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAIRNSTTLKELA 121
           TKAIQ+IC+PTD+KETCE+SLMAEA NTTDPKEL K GFK A   +N AIRNSTTL+EL+
Sbjct: 66  TKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQELS 125

Query: 122 KEPRTNQALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTCL 181
           K+PRTNQALQNCRELLEYAIDDL +SF RIGTF+MSKLDEFV DLKIWLSGALTYEQTCL
Sbjct: 126 KDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCL 185

Query: 182 DGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGATTGRRLL---DE 241
           DGFENTTGDAG +MQ+FLKSAQEMT+NGL IV ELSTI+GSL Q+PG  TGRRLL   D 
Sbjct: 186 DGFENTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSL-QIPG-MTGRRLLEDDDH 245

Query: 242 GVPSWVSEGKRRLMQAGPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYVKAG 301
            +PSW+S+GKRRLMQAG AG+KPD++VAQDGSGKYKTI+ AL DVP KSNKTFVIYVKAG
Sbjct: 246 EMPSWISDGKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAG 305

Query: 302 IYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGVQTFKTATFSTIGSDFFAKDMGFV 361
           IYKEIVV+PK +THLTMYGDGPTKTVVTGSLNFIDG+QTFKTATFS IG++F+A+DMGF 
Sbjct: 306 IYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFE 365

Query: 362 NSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAA 421
           N+AG+AKHQAVALRVQSDR+IFYNCQ+DGYQDTLYAHAHRQFYRDC+ISGTIDFVFGNAA
Sbjct: 366 NTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 425

Query: 422 AVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNKAYLG 481
             FQNCKL+VRKPMDNQQCIVTA GR+NR+EPTAL+FQ CHFMGDPAYLPFK++NKAYLG
Sbjct: 426 TNFQNCKLVVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKAYLG 485

Query: 482 RPWKEYSRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVKWRGI 541
           RPWK+YSRT+IIGSQIDD+IQPEGWLPW GDFGLNTLFYAEVQNKGPGA  +KRVKWRGI
Sbjct: 486 RPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKWRGI 545

Query: 542 KTITPDHAAKFTPRVFIDGDAWIKNKGIPYASGMM 574
           K ITP HAA FTPR FIDGDAWI  KGIPY+SGMM
Sbjct: 546 KHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 575

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BLAST of Spg024787 vs. NCBI nr
Match: XP_038875843.1 (probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida])

HSP 1 Score: 940.6 bits (2430), Expect = 6.1e-270
Identity = 461/575 (80.17%), Postives = 519/575 (90.26%), Query Frame = 0


Query: 2   SNAEGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHGDGGAEIKTS 61
           +NAEGDKKKK +AI+SVS+LILVAMVVAVTVGVN++P      E HGDGHG    EI ++
Sbjct: 6   ANAEGDKKKKKMAIISVSSLILVAMVVAVTVGVNNAPK-----EEHGDGHGGETHEISST 65

Query: 62  TKAIQSICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAIRNSTTLKELA 121
           TKAIQ+IC+PTD+K+ CE++LMAEA NTTDPKELAK G + A   +N AIRNSTTL+ELA
Sbjct: 66  TKAIQAICQPTDYKDVCEKNLMAEAGNTTDPKELAKVGLRIATRNLNEAIRNSTTLQELA 125

Query: 122 KEPRTNQALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTCL 181
           K+PRTNQALQNCRELLEYAIDDL +SF RIGTF+MSKLDEFV DLKIWLSGALTYEQTCL
Sbjct: 126 KDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCL 185

Query: 182 DGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGATTGRRLLDEG-- 241
           DGFENTTGDAG +MQ+FLKS+Q+MT+NGLAIV ELS ++GSL Q+PG  TGRRLL++   
Sbjct: 186 DGFENTTGDAGVRMQEFLKSSQQMTTNGLAIVNELSGMLGSL-QIPG-MTGRRLLEDDAD 245

Query: 242 -VPSWVSEGKRRLMQAGPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYVKAG 301
            +PSWVSEGKRRLMQA  A +KPD+VVAQDGSGKYKTI+ AL DVP KSNKTFVIYVKAG
Sbjct: 246 EMPSWVSEGKRRLMQAAAASMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAG 305

Query: 302 IYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGVQTFKTATFSTIGSDFFAKDMGFV 361
           IYKEIVV+PK +THLTMYGDGPTKT+VTGSLNFIDG+QTFKTATF+ IG++F+A+DMGF 
Sbjct: 306 IYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGIQTFKTATFAAIGANFYARDMGFE 365

Query: 362 NSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAA 421
           N+AGAAKHQAVALRVQSDR+IFYNC++DGYQDTLYAHAHRQFYRDC ISGTIDFVFGNAA
Sbjct: 366 NTAGAAKHQAVALRVQSDRSIFYNCKIDGYQDTLYAHAHRQFYRDCIISGTIDFVFGNAA 425

Query: 422 AVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNKAYLG 481
            +FQNCKL+VRKPMDNQQCIVTA GRLNR+EPTAL+FQSCHFMGDPAYLPFK +NKAYLG
Sbjct: 426 TIFQNCKLVVRKPMDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKNINKAYLG 485

Query: 482 RPWKEYSRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVKWRGI 541
           RPWK+YSRT+IIGSQIDD+IQPEGWLPW GDFGLNTLFYAEVQNKGPGAD SKRVKWRGI
Sbjct: 486 RPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGADESKRVKWRGI 545

Query: 542 KTITPDHAAKFTPRVFIDGDAWIKNKGIPYASGMM 574
           + ITP HAA +TPR FIDGDAWI  KGIPY+SGMM
Sbjct: 546 RHITPQHAADYTPRRFIDGDAWIPAKGIPYSSGMM 573

[Close]

BLAST of Spg024787 vs. NCBI nr
Match: XP_023549205.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 927.5 bits (2396), Expect = 5.4e-266
Identity = 450/576 (78.12%), Postives = 513/576 (89.06%), Query Frame = 0


Query: 3   NAEGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGD-DHGEGHGDG----HGDGGAE 62
           +AEGDKKKK IA++SVS+LILVAMVVAVTVGVN++P  D D G G G G    HGD G +
Sbjct: 6   DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHGDDGEK 65

Query: 63  IKTSTKAIQSICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAIRNSTTL 122
           + +STKAI++IC+PTD+KETCE+SLM EA NTTDPKEL KAGFK AVE +N AIRNSTTL
Sbjct: 66  VSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIRNSTTL 125

Query: 123 KELAKEPRTNQALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYE 182
           +ELA++PR NQALQNCRELLEYAIDDL  S++RIGTF +SK +EFVNDLKIWLS ALTYE
Sbjct: 126 QELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSAALTYE 185

Query: 183 QTCLDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGATTGRRLLD 242
           QTCLDGFENTTGD+G++M++FLKSAQEMT+NGL IV+ELS I+G+LH    A TGRRLLD
Sbjct: 186 QTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSNILGTLH--APAATGRRLLD 245

Query: 243 EGVPSWVSEGKRRLMQAGPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYVKA 302
             +P WVS+GKRRLMQ G   +KPD+VVAQDGSGKYKTI+EALVDVP KSNKTFVIYVKA
Sbjct: 246 TDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVDVPMKSNKTFVIYVKA 305

Query: 303 GIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGVQTFKTATFSTIGSDFFAKDMGF 362
           GIYKEIVV+PK +THLTMYGDGPTK+VVTG+LNF+DG+QTFKTATF+ IG++FF +DMGF
Sbjct: 306 GIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRDMGF 365

Query: 363 VNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNA 422
           VN+AG  KHQAVALRVQSDRAI YNCQ+DGYQDTLYAHA+RQFYRDC ISGTIDFVFGN+
Sbjct: 366 VNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVFGNS 425

Query: 423 AAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNKAYL 482
           AA+FQNCKL+VRKP+DNQQCIVTA GRLNRREPTAL+FQ CHFMGDPAYLPFK VNKAYL
Sbjct: 426 AAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPAYLPFKKVNKAYL 485

Query: 483 GRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVKWRG 542
           GRPWK+YSRT+I+GSQIDDLIQPEGWLPW GDF LNTLFYAEVQN+GPGAD S RVKWRG
Sbjct: 486 GRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVKWRG 545

Query: 543 IKTITPDHAAKFTPRVFIDGDAWIKNKGIPYASGMM 574
           I+ ITP HAA +T R FIDGDAWI  +G+PY+SGMM
Sbjct: 546 IRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

[Close]

BLAST of Spg024787 vs. NCBI nr
Match: XP_023006255.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita maxima])

HSP 1 Score: 924.1 bits (2387), Expect = 5.9e-265
Identity = 451/597 (75.54%), Postives = 516/597 (86.43%), Query Frame = 0


Query: 3   NAEGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGD-----------DHGEGHGD-- 62
           +AEGDKKKK IA++SVS+LILVAMVVAVTVGVN++PH D           DHG+  GD  
Sbjct: 6   DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPHEDLDDGGGGGDLHDHGDDGGDVH 65

Query: 63  -------------GHGDGGAEIKTSTKAIQSICRPTDFKETCEQSLMAEASNTTDPKELA 122
                         HGD G +I +STKAI++IC+PTD+KETCE+SLM EA NTTD KEL 
Sbjct: 66  DHGDYGGGGGDAHDHGDDGEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDLKELV 125

Query: 123 KAGFKAAVEYINTAIRNSTTLKELAKEPRTNQALQNCRELLEYAIDDLTQSFNRIGTFEM 182
           KAGFK AVE +N AIRNSTTL+ELAK+PR NQALQNCRELLEYAIDDL  S++RIGTF +
Sbjct: 126 KAGFKIAVEKLNAAIRNSTTLQELAKDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHV 185

Query: 183 SKLDEFVNDLKIWLSGALTYEQTCLDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTEL 242
           SK +EFVNDLKIWLS ALTYEQTCLDGFENTTGD+G++M++FLKSAQEMT+NGL IV+EL
Sbjct: 186 SKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSEL 245

Query: 243 STIMGSLHQMPGATTGRRLLDEGVPSWVSEGKRRLMQAGPAGLKPDVVVAQDGSGKYKTI 302
           S I+G+LH+   A TGRRLLD  +P WVS+GKRRLMQ     +KPD+VVAQDGSGKYKTI
Sbjct: 246 SNILGTLHE--PAATGRRLLDTDMPLWVSDGKRRLMQLETGAIKPDLVVAQDGSGKYKTI 305

Query: 303 SEALVDVPKKSNKTFVIYVKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGVQ 362
           +EALVDVP KSNKTFVIYVKAGIYKEIVV+PK +THLTMYGDGPTK+VVTG+LNF+DG+Q
Sbjct: 306 NEALVDVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQ 365

Query: 363 TFKTATFSTIGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHA 422
           TFKTATF+ IG++FF +DMGFVNSAG  KHQAVALR+QSDRAI YNCQ+DG+QDTLYAHA
Sbjct: 366 TFKTATFAAIGANFFVRDMGFVNSAGPTKHQAVALRIQSDRAIIYNCQIDGFQDTLYAHA 425

Query: 423 HRQFYRDCSISGTIDFVFGNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQ 482
           +RQFYRDC+ISGTIDFVFGN+AA+FQNCKL+VRKP+DNQQCIVTA GRLNRREPTAL+FQ
Sbjct: 426 NRQFYRDCTISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQ 485

Query: 483 SCHFMGDPAYLPFKAVNKAYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLF 542
            CHFMGDPAYLPFK VNKAYLGRPWK+YSRT+I+GSQIDDLIQPEGWLPW GDFGLNTLF
Sbjct: 486 GCHFMGDPAYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFGLNTLF 545

Query: 543 YAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKNKGIPYASGMM 574
           YAEVQN+GPGAD S RVKWRGI+ ITP HAA +T R FIDGDAWI  +G+PY+SGMM
Sbjct: 546 YAEVQNRGPGADQSHRVKWRGIRHITPQHAASYTARRFIDGDAWIPVRGVPYSSGMM 600

[Close]

BLAST of Spg024787 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 652.5 bits (1682), Expect = 4.4e-186
Identity = 307/568 (54.05%), Postives = 421/568 (74.12%), Query Frame = 0


Query: 8   KKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHGDGGAEIKTSTKAIQS 67
           K+K+   ++++S+++L++MVVAVTVGV+ + H         DG   G AE+  S KA++ 
Sbjct: 10  KRKRRYIVITISSVLLISMVVAVTVGVSLNKH---------DGDSKGKAEVNASVKAVKD 69

Query: 68  ICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAIRNSTTLKELAKEPRTN 127
           +C PTD+++TCE +L+    NTTDP EL K  F   ++ I  A + S T+ EL K+ RT 
Sbjct: 70  VCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTR 129

Query: 128 QALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTCLDGFENT 187
            AL  C+EL++YA+D+L+ SF  +G FE   LDE + +L+IWLS A+++E+TCL+GF+ T
Sbjct: 130 MALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGT 189

Query: 188 TGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGATTGRRLLDEGVPSWVSEG 247
            G+AG+ M++ LK+A E+T NGLAI++E+S  +G + Q+PG  + RRLL EG PSWV + 
Sbjct: 190 QGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQM-QIPGLNS-RRLLAEGFPSWVDQR 249

Query: 248 KRRLMQAGPA--GLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYVKAGIYKEIVV 307
            R+L+QA  A   +KPD+VVAQDGSG+YKTI+EAL  VPKK N TFV+++KAG+YKE V 
Sbjct: 250 GRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQ 309

Query: 308 VPKDVTHLTMYGDGPTKTVVTGSLNFIDGVQTFKTATFSTIGSDFFAKDMGFVNSAGAAK 367
           V K ++HL   GDGP KT+++G+ N+ DG+ T++TAT + +G+ F AK++GF N+AGA K
Sbjct: 310 VNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIK 369

Query: 368 HQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAAAVFQNCK 427
           HQAVA+RVQSD +IF+NC+ DGYQDTLY H+HRQF+RDC+ISGTIDF+FG+AAAVFQNC 
Sbjct: 370 HQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCT 429

Query: 428 LIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNKAYLGRPWKEYS 487
           L+VRKP+ NQ C +TA GR + RE T  VFQ C   G+P YL  K  +KAYLGRPWKEYS
Sbjct: 430 LLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYS 489

Query: 488 RTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVKWRGIKTITPDH 547
           RT+I+ + I D +QP+GW PW GDFGL TLFY+EVQN GPG+ L+ RV W GIKT++ + 
Sbjct: 490 RTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEED 549

Query: 548 AAKFTPRVFIDGDAWIKNKGIPYASGMM 574
             KFTP  +I GD WI  KG+PY +G++
Sbjct: 550 ILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

[Close]

BLAST of Spg024787 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 1.9e-181
Identity = 308/568 (54.23%), Postives = 412/568 (72.54%), Query Frame = 0


Query: 5   EGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHGDGGAEIKTSTKA 64
           E  K+KK   I+S+S+++L++MVVAVT+GV+ +           D  GD   EI TS KA
Sbjct: 8   EDAKRKKRYVIISISSVLLISMVVAVTIGVSVN---------KSDNAGD--EEITTSVKA 67

Query: 65  IQSICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAIRNSTTLKELAKEP 124
           I+ +C PTD+KETCE +L  +A +T+DP EL K  F A ++ I+   + S T+ EL K+P
Sbjct: 68  IKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDP 127

Query: 125 RTNQALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTCLDGF 184
           R   AL  C+EL++YAI +L++SF  +G FE  K+DE +  L+IWLS  +++EQTCLDGF
Sbjct: 128 RAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGF 187

Query: 185 ENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGATTGRRLLDEGVPSWV 244
           + T G+AG+ +++ LK+A ++T NGLA+VTE+S  +G + Q+P     RRLL +  PSW+
Sbjct: 188 QGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQM-QIP-EMNSRRLLSQEFPSWM 247

Query: 245 SEGKRRLMQAGPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYVKAGIYKEIV 304
               RRL+ A  + +KPD+VVAQDGSG+YKTI+EAL  VPKK N TFV+++K GIYKE V
Sbjct: 248 DARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYV 307

Query: 305 VVPKDVTHLTMYGDGPTKTVVTGSLNFIDGVQTFKTATFSTIGSDFFAKDMGFVNSAGAA 364
            V + +THL   GDGP KTV++GS ++ DG+ T+KTAT + +G  F AK++ F N+AGA 
Sbjct: 308 QVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAI 367

Query: 365 KHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAAAVFQNC 424
           KHQAVA+RV +D +IFYNC+ DGYQDTLYAH+HRQFYRDC+ISGTIDF+FG+AAAVFQNC
Sbjct: 368 KHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNC 427

Query: 425 KLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNKAYLGRPWKEY 484
            L+VRKP+ NQ C +TA GR + RE T  V Q C  +G+P YL  K  +K YLGRPWKEY
Sbjct: 428 TLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEY 487

Query: 485 SRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVKWRGIKTITPD 544
           SRT+I+ + I D + PEGW PW G+FGLNTLFY+EVQN GPGA ++KRV W GIK ++ +
Sbjct: 488 SRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDE 547

Query: 545 HAAKFTPRVFIDGDAWIKNKGIPYASGM 573
              KFTP  +I GDAWI  KG+PY  G+
Sbjct: 548 EILKFTPAQYIQGDAWIPGKGVPYILGL 562

[Close]

BLAST of Spg024787 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 6.2e-164
Identity = 285/574 (49.65%), Postives = 397/574 (69.16%), Query Frame = 0


Query: 5   EGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHGDGGAEIKTSTKA 64
           +G+ KKK   I  V   +LV MVVA  VG+ +S     H E           +IKT+T A
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVA--VGITTS-RNTSHSEKIVP------VQIKTATTA 63

Query: 65  IQSICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAIRNSTT--LKELAK 124
           ++++C PTD+KETC  SLM  + ++T P +L K GF   +  I  +I+ ++     + A 
Sbjct: 64  VEAVCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAAN 123

Query: 125 EPRTNQALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTCLD 184
           +  T  AL+ C +L+  A DDL +  +    F + ++++FV DL++WLSG++ Y+QTC+D
Sbjct: 124 DKDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMD 183

Query: 185 GFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGAT-----TGRRLL- 244
            FE T     Q MQ+  K+++E+TSNGLA++T +S ++G  + + G T       R+LL 
Sbjct: 184 TFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFN-VTGVTGDLGKYARKLLS 243

Query: 245 -DEGVPSWVSEGKRRLMQAGPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYV 304
            ++G+PSWV    RRLM A   G+K +VVVA DGSG+YKTI+EAL  VPK + K FVIY+
Sbjct: 244 AEDGIPSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYI 303

Query: 305 KAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDG-VQTFKTATFSTIGSDFFAKD 364
           K G+Y E V V K +TH+T  GDGPTKT +TGSLN+  G V+T+ TAT +  G +F AK+
Sbjct: 304 KQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKN 363

Query: 365 MGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVF 424
           +GF N+AG   HQAVALRV +D A+FYNCQ+DGYQDTLY H+HRQF+RDC++SGT+DF+F
Sbjct: 364 IGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIF 423

Query: 425 GNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNK 484
           G+   V QNC ++VRKPM +Q C++TAQGR ++RE T LV Q+CH  G+PAY+P K++NK
Sbjct: 424 GDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINK 483

Query: 485 AYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVK 544
           AYLGRPWKE+SRT+I+G+ IDD+I P GWLPW GDF LNTL+YAE +N GPG++ ++RVK
Sbjct: 484 AYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVK 543

Query: 545 WRGIKTITPDHAAKFTPRVFIDGDAWIKNKGIPY 569
           W GIK ++P  A +FTP  F+ G+ WI    +PY
Sbjct: 544 WPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

[Close]

BLAST of Spg024787 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 542.3 bits (1396), Expect = 6.4e-153
Identity = 277/579 (47.84%), Postives = 388/579 (67.01%), Query Frame = 0


Query: 4   AEGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHGDGGAEIKTSTK 63
           ++GDKKKK I   SVS  +   +++ V+V V +S H     E H          I+ +TK
Sbjct: 3   SDGDKKKKFIVAGSVSGFL---VIMVVSVAVVTSKHSPKDDENH----------IRKTTK 62

Query: 64  AIQSICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAI-RNSTTLKELA- 123
           A+Q++C PTDFK+TC  SLM  + ++ DP +L K GFK  ++ IN ++ + S  +K  A 
Sbjct: 63  AVQAVCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKAD 122

Query: 124 KEPRTNQALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTCL 183
           K P    A + C +L+  AIDDL +  +    F + +++ FV DL++WLSG++ ++QTC+
Sbjct: 123 KNPEAKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCM 182

Query: 184 DGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIM--GSLHQMPGATT--GRRLL- 243
           D F     +  Q M +  K+++E++SN LA+VT +ST++   +L  + GA     R+LL 
Sbjct: 183 DSFGEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLS 242

Query: 244 -DEGVPSWVSEGKRRLMQA---GPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFV 303
            ++ +P+WV    RRLM A   GP  +K + VVAQDG+G++KTI++AL  VPK +   F+
Sbjct: 243 TEDSIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFI 302

Query: 304 IYVKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNF-IDGVQTFKTATFSTIGSDFF 363
           I++K GIYKE V V K + H+T  GDGP KT++TGSLNF I  V+TF TAT +  G  F 
Sbjct: 303 IHIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFT 362

Query: 364 AKDMGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTID 423
           AK++G  N+AG    QAVALRV +D A+F++CQ+DG+QDTLY H+HRQFYRDC++SGT+D
Sbjct: 363 AKNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVD 422

Query: 424 FVFGNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKA 483
           F+FG+A  + QNCK++VRKP   Q C+VTAQGR N RE T LV   CH  GDPAY+P K+
Sbjct: 423 FIFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKS 482

Query: 484 VNKAYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSK 543
           VNKAYLGRPWKE+SRT+I+ + IDD+I P GWLPW GDF L TL+YAE  N GPG++ ++
Sbjct: 483 VNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQ 542

Query: 544 RVKWRGIKTITPDHAAKFTPRVFIDGDAWIKNKGIPYAS 571
           RVKW GIK +TP  A  +T   F+ GD WI    +PY +
Sbjct: 543 RVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

[Close]

BLAST of Spg024787 vs. ExPASy Swiss-Prot
Match: Q42920 (Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 5.1e-134
Identity = 230/450 (51.11%), Postives = 312/450 (69.33%), Query Frame = 0


Query: 130 LQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTCLDGFENTTG 189
           ++ C E+L+YA+D + +S   +  F+  KL E+  DLK+WL+G L+++QTCLDGF NTT 
Sbjct: 1   MEICNEVLDYAVDGIHKSVGTLDQFDFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTTT 60

Query: 190 DAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGATTGRRLLDEGVPSWVSEGKR 249
            AG+ M + LK++ E++SN + ++  +S I+        + + R L D+G+PSWV++G R
Sbjct: 61  KAGETMTKVLKTSMELSSNAIDMMDAVSRILKGFDTSQYSVSRRLLSDDGIPSWVNDGHR 120

Query: 250 RLMQAGPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYVKAGIYKEIVVVPKD 309
           RL+  G   ++P+ VVAQDGSG++KT+++AL  VP K+   FVI+VKAG+YKE V V K+
Sbjct: 121 RLLAGG--NVQPNAVVAQDGSGQFKTLTDALKTVPPKNAVPFVIHVKAGVYKETVNVAKE 180

Query: 310 VTHLTMYGDGPTKTVVTGSLNFIDGVQTFKTATFSTIGSDFFAKDMGFVNSAGAAKHQAV 369
           + ++T+ GDGPTKT  TGSLN+ DG+ T+ TATF   G++F AKD+GF N+AG  KHQAV
Sbjct: 181 MNYVTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAGTGKHQAV 240

Query: 370 ALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAAAVFQNCKLIVR 429
           ALRV +D+AIFYNCQMDG+QDTLY  + RQFYRDCSISGTIDFVFG    VFQNCKL+ R
Sbjct: 241 ALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQNCKLVCR 300

Query: 430 KPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNKAYLGRPWKEYSRTLI 489
            P   QQC+VTA GR  +   +ALVFQS HF G+PA L       +YLGRPWK+YS+ +I
Sbjct: 301 LPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPA-LTSVTPKVSYLGRPWKQYSKVVI 360

Query: 490 IGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKF 549
           + S ID +  PEG++PW G     T  Y E  NKGPGAD + RVKW G+K +T + AA++
Sbjct: 361 MDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVKWHGVKVLTSNVAAEY 420

Query: 550 TPRVFID------GDAWIKNKGIPYASGMM 574
            P  F +       D WI   G+PY+ G M
Sbjct: 421 YPGKFFEIVNATARDTWIVKSGVPYSLGPM 447

[Close]

BLAST of Spg024787 vs. ExPASy TrEMBL
Match: A0A0A0KD24 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 2.9e-273
Identity = 470/576 (81.60%), Postives = 522/576 (90.62%), Query Frame = 0


Query: 2   SNAEGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHG-DGGAEIKT 61
           +NAEGDKKKK IAI+SVS+LILVAMVVAVTVGVN++PHG++HG+   D HG +   EI +
Sbjct: 6   ANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGD---DNHGSETTPEITS 65

Query: 62  STKAIQSICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAIRNSTTLKEL 121
           STKAIQ+IC+PTD+KETCE+SL AEA NTTDPKEL K GFK A   +N AI+NSTTLKEL
Sbjct: 66  STKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLKEL 125

Query: 122 AKEPRTNQALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTC 181
           AK+PRTNQALQNCRELLEYAIDDL QSF RIGTF+MSKLD+FV DLKIWLSGALTYEQTC
Sbjct: 126 AKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQTC 185

Query: 182 LDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGATTGRRLL---D 241
           LDGFENTTGDAG +MQ+FLKSAQ+MT+NGL IV ELSTI+GSL Q+PG  +GRRLL   D
Sbjct: 186 LDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSL-QLPG-MSGRRLLEDDD 245

Query: 242 EGVPSWVSEGKRRLMQAGPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYVKA 301
             +PSWVS+GKRRLMQAG A +KPD+VVAQDGSGKYKTI+ AL DVP KSNKTFVI+VKA
Sbjct: 246 REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA 305

Query: 302 GIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGVQTFKTATFSTIGSDFFAKDMGF 361
           GIYKEIVV+PK +THLTMYGDGPTKTVVTGSLNFIDG+QTFKTATFS IG++F+A+DMGF
Sbjct: 306 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 365

Query: 362 VNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNA 421
            N+AGAAKHQAVALRVQSDR+IF+NCQ+DGYQDTLYAHAHRQFYRDC+ISGTIDFVFGNA
Sbjct: 366 ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 425

Query: 422 AAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNKAYL 481
           A  FQNCKL+VRKP+DNQQCIVTA GRLNR+EPTAL+FQSCHFMGDPAYLPFKA+NKAYL
Sbjct: 426 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL 485

Query: 482 GRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVKWRG 541
           GRPWKEYSRT+IIGS IDD+IQPEGWLPW GDFGLNTLFYAEVQNKG GAD SKRVKWRG
Sbjct: 486 GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG 545

Query: 542 IKTITPDHAAKFTPRVFIDGDAWIKNKGIPYASGMM 574
           IK ITP HAA FTPR FIDGDAWI  KGIPY+SGMM
Sbjct: 546 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

[Close]

BLAST of Spg024787 vs. ExPASy TrEMBL
Match: A0A1S3C7B6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 5.4e-272
Identity = 466/575 (81.04%), Postives = 521/575 (90.61%), Query Frame = 0


Query: 2   SNAEGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHGDGGAEIKTS 61
           +NAEGDKKKK IAI+SVS+LILVAMVVAVTVG+N++P+ ++HGE  GD       EI +S
Sbjct: 6   ANAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPY-EEHGE--GDQGSKTTPEISSS 65

Query: 62  TKAIQSICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAIRNSTTLKELA 121
           TKAIQ+IC+PTD+KETCE+SLMAEA NTTDPKEL K GFK A   +N AIRNSTTL+EL+
Sbjct: 66  TKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQELS 125

Query: 122 KEPRTNQALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTCL 181
           K+PRTNQALQNCRELLEYAIDDL +SF RIGTF+MSKLDEFV DLKIWLSGALTYEQTCL
Sbjct: 126 KDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCL 185

Query: 182 DGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGATTGRRLL---DE 241
           DGFENTTGDAG +MQ+FLKSAQEMT+NGL IV ELSTI+GSL Q+PG  TGRRLL   D 
Sbjct: 186 DGFENTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSL-QIPG-MTGRRLLEDDDH 245

Query: 242 GVPSWVSEGKRRLMQAGPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYVKAG 301
            +PSW+S+GKRRLMQAG AG+KPD++VAQDGSGKYKTI+ AL DVP KSNKTFVIYVKAG
Sbjct: 246 EMPSWISDGKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAG 305

Query: 302 IYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGVQTFKTATFSTIGSDFFAKDMGFV 361
           IYKEIVV+PK +THLTMYGDGPTKTVVTGSLNFIDG+QTFKTATFS IG++F+A+DMGF 
Sbjct: 306 IYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFE 365

Query: 362 NSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAA 421
           N+AG+AKHQAVALRVQSDR+IFYNCQ+DGYQDTLYAHAHRQFYRDC+ISGTIDFVFGNAA
Sbjct: 366 NTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 425

Query: 422 AVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNKAYLG 481
             FQNCKL+VRKPMDNQQCIVTA GR+NR+EPTAL+FQ CHFMGDPAYLPFK++NKAYLG
Sbjct: 426 TNFQNCKLVVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKAYLG 485

Query: 482 RPWKEYSRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVKWRGI 541
           RPWK+YSRT+IIGSQIDD+IQPEGWLPW GDFGLNTLFYAEVQNKGPGA  +KRVKWRGI
Sbjct: 486 RPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKWRGI 545

Query: 542 KTITPDHAAKFTPRVFIDGDAWIKNKGIPYASGMM 574
           K ITP HAA FTPR FIDGDAWI  KGIPY+SGMM
Sbjct: 546 KHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 575

[Close]

BLAST of Spg024787 vs. ExPASy TrEMBL
Match: A0A6J1KZN0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499042 PE=3 SV=1)

HSP 1 Score: 924.1 bits (2387), Expect = 2.9e-265
Identity = 451/597 (75.54%), Postives = 516/597 (86.43%), Query Frame = 0


Query: 3   NAEGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGD-----------DHGEGHGD-- 62
           +AEGDKKKK IA++SVS+LILVAMVVAVTVGVN++PH D           DHG+  GD  
Sbjct: 6   DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPHEDLDDGGGGGDLHDHGDDGGDVH 65

Query: 63  -------------GHGDGGAEIKTSTKAIQSICRPTDFKETCEQSLMAEASNTTDPKELA 122
                         HGD G +I +STKAI++IC+PTD+KETCE+SLM EA NTTD KEL 
Sbjct: 66  DHGDYGGGGGDAHDHGDDGEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDLKELV 125

Query: 123 KAGFKAAVEYINTAIRNSTTLKELAKEPRTNQALQNCRELLEYAIDDLTQSFNRIGTFEM 182
           KAGFK AVE +N AIRNSTTL+ELAK+PR NQALQNCRELLEYAIDDL  S++RIGTF +
Sbjct: 126 KAGFKIAVEKLNAAIRNSTTLQELAKDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHV 185

Query: 183 SKLDEFVNDLKIWLSGALTYEQTCLDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTEL 242
           SK +EFVNDLKIWLS ALTYEQTCLDGFENTTGD+G++M++FLKSAQEMT+NGL IV+EL
Sbjct: 186 SKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSEL 245

Query: 243 STIMGSLHQMPGATTGRRLLDEGVPSWVSEGKRRLMQAGPAGLKPDVVVAQDGSGKYKTI 302
           S I+G+LH+   A TGRRLLD  +P WVS+GKRRLMQ     +KPD+VVAQDGSGKYKTI
Sbjct: 246 SNILGTLHE--PAATGRRLLDTDMPLWVSDGKRRLMQLETGAIKPDLVVAQDGSGKYKTI 305

Query: 303 SEALVDVPKKSNKTFVIYVKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGVQ 362
           +EALVDVP KSNKTFVIYVKAGIYKEIVV+PK +THLTMYGDGPTK+VVTG+LNF+DG+Q
Sbjct: 306 NEALVDVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQ 365

Query: 363 TFKTATFSTIGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHA 422
           TFKTATF+ IG++FF +DMGFVNSAG  KHQAVALR+QSDRAI YNCQ+DG+QDTLYAHA
Sbjct: 366 TFKTATFAAIGANFFVRDMGFVNSAGPTKHQAVALRIQSDRAIIYNCQIDGFQDTLYAHA 425

Query: 423 HRQFYRDCSISGTIDFVFGNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQ 482
           +RQFYRDC+ISGTIDFVFGN+AA+FQNCKL+VRKP+DNQQCIVTA GRLNRREPTAL+FQ
Sbjct: 426 NRQFYRDCTISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQ 485

Query: 483 SCHFMGDPAYLPFKAVNKAYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLF 542
            CHFMGDPAYLPFK VNKAYLGRPWK+YSRT+I+GSQIDDLIQPEGWLPW GDFGLNTLF
Sbjct: 486 GCHFMGDPAYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFGLNTLF 545

Query: 543 YAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKNKGIPYASGMM 574
           YAEVQN+GPGAD S RVKWRGI+ ITP HAA +T R FIDGDAWI  +G+PY+SGMM
Sbjct: 546 YAEVQNRGPGADQSHRVKWRGIRHITPQHAASYTARRFIDGDAWIPVRGVPYSSGMM 600

[Close]

BLAST of Spg024787 vs. ExPASy TrEMBL
Match: A0A6J1H4H8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 4.6e-263
Identity = 446/576 (77.43%), Postives = 509/576 (88.37%), Query Frame = 0


Query: 3   NAEGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGD-DHGEGHGDG----HGDGGAE 62
           +AE DKKKK IA++SVS+LILVAMVVAVTVGVN++P  D D G G G G    HGD   +
Sbjct: 6   DAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHGDDAEK 65

Query: 63  IKTSTKAIQSICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAIRNSTTL 122
           I +STKAI++IC+PTD+KETCE+SLM EA NTTDPKEL KAGFK AVE +N AIRNSTTL
Sbjct: 66  ISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIRNSTTL 125

Query: 123 KELAKEPRTNQALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYE 182
           +ELA++PR NQALQNCRELLEYAIDDL  S++RIGTF +SK +EFVNDLKIWLS ALTYE
Sbjct: 126 QELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSAALTYE 185

Query: 183 QTCLDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGATTGRRLLD 242
           QTCLDGFENTTGD+G++M++FLKSAQEMT+NGL IV+ELS I+G+L     A TGRRLLD
Sbjct: 186 QTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLD--APAATGRRLLD 245

Query: 243 EGVPSWVSEGKRRLMQAGPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYVKA 302
             +P WVS+GKRRLMQ G   +KPD+VVAQDGSGKYKTI+EALV VP KSNKTFVIYVKA
Sbjct: 246 TDIPLWVSDGKRRLMQVGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIYVKA 305

Query: 303 GIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGVQTFKTATFSTIGSDFFAKDMGF 362
           GIYKEIVV+PK +THLTMYGDGPTK+VVTG+LNF+DG+QTFKTATF+ IG++FF +DMGF
Sbjct: 306 GIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRDMGF 365

Query: 363 VNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNA 422
           VN+AG  KHQAVALRVQSDRAI YNCQ+DGYQDTLYAHA+RQFYRDC ISGTIDFVFGN+
Sbjct: 366 VNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVFGNS 425

Query: 423 AAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNKAYL 482
           AA+FQNCKL+VRKP+DNQQCIVTA GRLNRREPTAL+FQ CHFMGDP+YLPFK VNKAYL
Sbjct: 426 AAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNKAYL 485

Query: 483 GRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVKWRG 542
           GRPWK+YSRT+I+GSQIDDLIQPEGWLPW GDF LNTLFYAEVQN+GPGAD S RVKWRG
Sbjct: 486 GRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVKWRG 545

Query: 543 IKTITPDHAAKFTPRVFIDGDAWIKNKGIPYASGMM 574
           I+ ITP HAA +T R FIDGDAWI  +G+PY+SGMM
Sbjct: 546 IRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

[Close]

BLAST of Spg024787 vs. ExPASy TrEMBL
Match: A0A6J1KJ31 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111494433 PE=3 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 2.1e-260
Identity = 439/572 (76.75%), Postives = 502/572 (87.76%), Query Frame = 0


Query: 3   NAEGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHGDGGAEIKTST 62
           +AEGD KKK +A V VS+L LVAMVVAVTVGVN+S H    GEG GDGHGDG  EI +ST
Sbjct: 6   DAEGDNKKKKMAFVGVSSLFLVAMVVAVTVGVNNSSH---KGEGLGDGHGDGAGEISSST 65

Query: 63  KAIQSICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAIRNSTTLKELAK 122
           KAIQ+ICRPTD+KETCE+SLM+E SNTTDP+EL K GFK  ++ +N+AIRNSTTL+ LAK
Sbjct: 66  KAIQTICRPTDYKETCEKSLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAK 125

Query: 123 EPRTNQALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTCLD 182
           EPRTNQALQNCRELLEYAIDD+T SFNRIGTF+M+KLD+FV DL IWLSGALTYEQTCLD
Sbjct: 126 EPRTNQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSIWLSGALTYEQTCLD 185

Query: 183 GFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGATTGRRLL-DEGVP 242
           GFENTTGDAG++M+ FLKSAQEMTSNGLAIVTEL  ++GSL    G+  GRRLL  + VP
Sbjct: 186 GFENTTGDAGERMKMFLKSAQEMTSNGLAIVTELKNMLGSLQ---GSKAGRRLLAGDEVP 245

Query: 243 SWVSEGKRRLMQAGPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYVKAGIYK 302
           SWVS  KRRLMQ   + + P+VVVAQDGSGKYKTI+EALVDVPKKSN TFVIYVKAGIYK
Sbjct: 246 SWVSSEKRRLMQLPRSAITPNVVVAQDGSGKYKTINEALVDVPKKSNITFVIYVKAGIYK 305

Query: 303 EIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGVQTFKTATFSTIGSDFFAKDMGFVNSA 362
           E V++PK +TH+TMYGDGPTKT+V+G+LNF+DG+QTFKTATF+ +G++FF +DMGF N+A
Sbjct: 306 ETVLIPKALTHVTMYGDGPTKTIVSGALNFVDGIQTFKTATFAVVGANFFCRDMGFENTA 365

Query: 363 GAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAAAVF 422
           G+AKHQAVALRVQSDR+IF+NCQMDGYQDTLYA  HRQFYRDC +SGTIDFVFGN+AAVF
Sbjct: 366 GSAKHQAVALRVQSDRSIFFNCQMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVF 425

Query: 423 QNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNKAYLGRPW 482
           QNC L+VRKP+ NQQCIVTAQGRL  REPT  VFQ CH MGDPAYLPFKAVNKAYLGRPW
Sbjct: 426 QNCNLVVRKPLANQQCIVTAQGRLEAREPTGFVFQKCHLMGDPAYLPFKAVNKAYLGRPW 485

Query: 483 KEYSRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVKWRGIKTI 542
           K+YSRT+I+GS+IDDLIQPEGWLPW GDFGLN+LFYAE QNKGPGADL+ RVKW+GIKT+
Sbjct: 486 KQYSRTIIMGSEIDDLIQPEGWLPWMGDFGLNSLFYAEFQNKGPGADLTNRVKWQGIKTL 545

Query: 543 TPDHAAKFTPRVFIDGDAWIKNKGIPYASGMM 574
           T   AA FTP+VFIDGDAWI N G+PY+ GMM
Sbjct: 546 TAQQAADFTPKVFIDGDAWILNTGVPYSPGMM 571

[Close]

BLAST of Spg024787 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 652.5 bits (1682), Expect = 3.1e-187
Identity = 307/568 (54.05%), Postives = 421/568 (74.12%), Query Frame = 0


Query: 8   KKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHGDGGAEIKTSTKAIQS 67
           K+K+   ++++S+++L++MVVAVTVGV+ + H         DG   G AE+  S KA++ 
Sbjct: 10  KRKRRYIVITISSVLLISMVVAVTVGVSLNKH---------DGDSKGKAEVNASVKAVKD 69

Query: 68  ICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAIRNSTTLKELAKEPRTN 127
           +C PTD+++TCE +L+    NTTDP EL K  F   ++ I  A + S T+ EL K+ RT 
Sbjct: 70  VCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTR 129

Query: 128 QALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTCLDGFENT 187
            AL  C+EL++YA+D+L+ SF  +G FE   LDE + +L+IWLS A+++E+TCL+GF+ T
Sbjct: 130 MALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGT 189

Query: 188 TGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGATTGRRLLDEGVPSWVSEG 247
            G+AG+ M++ LK+A E+T NGLAI++E+S  +G + Q+PG  + RRLL EG PSWV + 
Sbjct: 190 QGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQM-QIPGLNS-RRLLAEGFPSWVDQR 249

Query: 248 KRRLMQAGPA--GLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYVKAGIYKEIVV 307
            R+L+QA  A   +KPD+VVAQDGSG+YKTI+EAL  VPKK N TFV+++KAG+YKE V 
Sbjct: 250 GRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQ 309

Query: 308 VPKDVTHLTMYGDGPTKTVVTGSLNFIDGVQTFKTATFSTIGSDFFAKDMGFVNSAGAAK 367
           V K ++HL   GDGP KT+++G+ N+ DG+ T++TAT + +G+ F AK++GF N+AGA K
Sbjct: 310 VNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIK 369

Query: 368 HQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAAAVFQNCK 427
           HQAVA+RVQSD +IF+NC+ DGYQDTLY H+HRQF+RDC+ISGTIDF+FG+AAAVFQNC 
Sbjct: 370 HQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCT 429

Query: 428 LIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNKAYLGRPWKEYS 487
           L+VRKP+ NQ C +TA GR + RE T  VFQ C   G+P YL  K  +KAYLGRPWKEYS
Sbjct: 430 LLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYS 489

Query: 488 RTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVKWRGIKTITPDH 547
           RT+I+ + I D +QP+GW PW GDFGL TLFY+EVQN GPG+ L+ RV W GIKT++ + 
Sbjct: 490 RTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEED 549

Query: 548 AAKFTPRVFIDGDAWIKNKGIPYASGMM 574
             KFTP  +I GD WI  KG+PY +G++
Sbjct: 550 ILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

[Close]

BLAST of Spg024787 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 637.1 bits (1642), Expect = 1.4e-182
Identity = 308/568 (54.23%), Postives = 412/568 (72.54%), Query Frame = 0


Query: 5   EGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHGDGGAEIKTSTKA 64
           E  K+KK   I+S+S+++L++MVVAVT+GV+ +           D  GD   EI TS KA
Sbjct: 8   EDAKRKKRYVIISISSVLLISMVVAVTIGVSVN---------KSDNAGD--EEITTSVKA 67

Query: 65  IQSICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAIRNSTTLKELAKEP 124
           I+ +C PTD+KETCE +L  +A +T+DP EL K  F A ++ I+   + S T+ EL K+P
Sbjct: 68  IKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDP 127

Query: 125 RTNQALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTCLDGF 184
           R   AL  C+EL++YAI +L++SF  +G FE  K+DE +  L+IWLS  +++EQTCLDGF
Sbjct: 128 RAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGF 187

Query: 185 ENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGATTGRRLLDEGVPSWV 244
           + T G+AG+ +++ LK+A ++T NGLA+VTE+S  +G + Q+P     RRLL +  PSW+
Sbjct: 188 QGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQM-QIP-EMNSRRLLSQEFPSWM 247

Query: 245 SEGKRRLMQAGPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYVKAGIYKEIV 304
               RRL+ A  + +KPD+VVAQDGSG+YKTI+EAL  VPKK N TFV+++K GIYKE V
Sbjct: 248 DARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYV 307

Query: 305 VVPKDVTHLTMYGDGPTKTVVTGSLNFIDGVQTFKTATFSTIGSDFFAKDMGFVNSAGAA 364
            V + +THL   GDGP KTV++GS ++ DG+ T+KTAT + +G  F AK++ F N+AGA 
Sbjct: 308 QVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAI 367

Query: 365 KHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAAAVFQNC 424
           KHQAVA+RV +D +IFYNC+ DGYQDTLYAH+HRQFYRDC+ISGTIDF+FG+AAAVFQNC
Sbjct: 368 KHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNC 427

Query: 425 KLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNKAYLGRPWKEY 484
            L+VRKP+ NQ C +TA GR + RE T  V Q C  +G+P YL  K  +K YLGRPWKEY
Sbjct: 428 TLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEY 487

Query: 485 SRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVKWRGIKTITPD 544
           SRT+I+ + I D + PEGW PW G+FGLNTLFY+EVQN GPGA ++KRV W GIK ++ +
Sbjct: 488 SRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDE 547

Query: 545 HAAKFTPRVFIDGDAWIKNKGIPYASGM 573
              KFTP  +I GDAWI  KG+PY  G+
Sbjct: 548 EILKFTPAQYIQGDAWIPGKGVPYILGL 562

[Close]

BLAST of Spg024787 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 578.9 bits (1491), Expect = 4.4e-165
Identity = 285/574 (49.65%), Postives = 397/574 (69.16%), Query Frame = 0


Query: 5   EGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHGDGGAEIKTSTKA 64
           +G+ KKK   I  V   +LV MVVA  VG+ +S     H E           +IKT+T A
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVA--VGITTS-RNTSHSEKIVP------VQIKTATTA 63

Query: 65  IQSICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAIRNSTT--LKELAK 124
           ++++C PTD+KETC  SLM  + ++T P +L K GF   +  I  +I+ ++     + A 
Sbjct: 64  VEAVCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAAN 123

Query: 125 EPRTNQALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTCLD 184
           +  T  AL+ C +L+  A DDL +  +    F + ++++FV DL++WLSG++ Y+QTC+D
Sbjct: 124 DKDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMD 183

Query: 185 GFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLHQMPGAT-----TGRRLL- 244
            FE T     Q MQ+  K+++E+TSNGLA++T +S ++G  + + G T       R+LL 
Sbjct: 184 TFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFN-VTGVTGDLGKYARKLLS 243

Query: 245 -DEGVPSWVSEGKRRLMQAGPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFVIYV 304
            ++G+PSWV    RRLM A   G+K +VVVA DGSG+YKTI+EAL  VPK + K FVIY+
Sbjct: 244 AEDGIPSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYI 303

Query: 305 KAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDG-VQTFKTATFSTIGSDFFAKD 364
           K G+Y E V V K +TH+T  GDGPTKT +TGSLN+  G V+T+ TAT +  G +F AK+
Sbjct: 304 KQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKN 363

Query: 365 MGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVF 424
           +GF N+AG   HQAVALRV +D A+FYNCQ+DGYQDTLY H+HRQF+RDC++SGT+DF+F
Sbjct: 364 IGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIF 423

Query: 425 GNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKAVNK 484
           G+   V QNC ++VRKPM +Q C++TAQGR ++RE T LV Q+CH  G+PAY+P K++NK
Sbjct: 424 GDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINK 483

Query: 485 AYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSKRVK 544
           AYLGRPWKE+SRT+I+G+ IDD+I P GWLPW GDF LNTL+YAE +N GPG++ ++RVK
Sbjct: 484 AYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVK 543

Query: 545 WRGIKTITPDHAAKFTPRVFIDGDAWIKNKGIPY 569
           W GIK ++P  A +FTP  F+ G+ WI    +PY
Sbjct: 544 WPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

[Close]

BLAST of Spg024787 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 542.3 bits (1396), Expect = 4.5e-154
Identity = 277/579 (47.84%), Postives = 388/579 (67.01%), Query Frame = 0


Query: 4   AEGDKKKKNIAIVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHGDGGAEIKTSTK 63
           ++GDKKKK I   SVS  +   +++ V+V V +S H     E H          I+ +TK
Sbjct: 3   SDGDKKKKFIVAGSVSGFL---VIMVVSVAVVTSKHSPKDDENH----------IRKTTK 62

Query: 64  AIQSICRPTDFKETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAI-RNSTTLKELA- 123
           A+Q++C PTDFK+TC  SLM  + ++ DP +L K GFK  ++ IN ++ + S  +K  A 
Sbjct: 63  AVQAVCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKAD 122

Query: 124 KEPRTNQALQNCRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTCL 183
           K P    A + C +L+  AIDDL +  +    F + +++ FV DL++WLSG++ ++QTC+
Sbjct: 123 KNPEAKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCM 182

Query: 184 DGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIM--GSLHQMPGATT--GRRLL- 243
           D F     +  Q M +  K+++E++SN LA+VT +ST++   +L  + GA     R+LL 
Sbjct: 183 DSFGEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLS 242

Query: 244 -DEGVPSWVSEGKRRLMQA---GPAGLKPDVVVAQDGSGKYKTISEALVDVPKKSNKTFV 303
            ++ +P+WV    RRLM A   GP  +K + VVAQDG+G++KTI++AL  VPK +   F+
Sbjct: 243 TEDSIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFI 302

Query: 304 IYVKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNF-IDGVQTFKTATFSTIGSDFF 363
           I++K GIYKE V V K + H+T  GDGP KT++TGSLNF I  V+TF TAT +  G  F 
Sbjct: 303 IHIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFT 362

Query: 364 AKDMGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTID 423
           AK++G  N+AG    QAVALRV +D A+F++CQ+DG+QDTLY H+HRQFYRDC++SGT+D
Sbjct: 363 AKNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVD 422

Query: 424 FVFGNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPAYLPFKA 483
           F+FG+A  + QNCK++VRKP   Q C+VTAQGR N RE T LV   CH  GDPAY+P K+
Sbjct: 423 FIFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKS 482

Query: 484 VNKAYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGDFGLNTLFYAEVQNKGPGADLSK 543
           VNKAYLGRPWKE+SRT+I+ + IDD+I P GWLPW GDF L TL+YAE  N GPG++ ++
Sbjct: 483 VNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQ 542

Query: 544 RVKWRGIKTITPDHAAKFTPRVFIDGDAWIKNKGIPYAS 571
           RVKW GIK +TP  A  +T   F+ GD WI    +PY +
Sbjct: 543 RVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

[Close]

BLAST of Spg024787 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 459.9 bits (1182), Expect = 3.0e-129
Identity = 275/703 (39.12%), Postives = 376/703 (53.49%), Query Frame = 0


Query: 15  IVSVSALILVAMVVAVTVGVNSSPHGDDHGEGHGDGHGDGGAEIKTSTKAIQSICRPTDF 74
           ++ V+ALI+ AMV+ V     +SP          + H +    I  +T +I  IC  TD+
Sbjct: 6   LLGVTALIM-AMVICVEANDGNSP-----SRKMEEVHRESKLMITKTTVSI--ICASTDY 65

Query: 75  KETCEQSLMAEASNTTDPKELAKAGFKAAVEYINTAI-RNSTTLKELA-KEPRTNQALQN 134
           K+ C  SL    S   DP+ L ++ F  A+  I + I R    LK  A  +  T +AL  
Sbjct: 66  KQDCTTSLATVRS--PDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNT 125

Query: 135 CRELLEYAIDDLTQSFNRIGTFEMSKLDEFVNDLKIWLSGALTYEQTCLDGFENTTGDAG 194
           CREL++ AIDDL ++ ++   F  ++L +FV DL +WLSG++TY+QTC+DGFE    +A 
Sbjct: 126 CRELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAA 185

Query: 195 QKMQQFLKSAQEMTSNGLAIVTEL------------------------------------ 254
             M++ ++  Q +TSNGLAI   L                                    
Sbjct: 186 VMMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGS 245

Query: 255 ---------------STIMGSLHQ------------------------------------ 314
                          ST+  S +Q                                    
Sbjct: 246 SQDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKK 305

Query: 315 ---------------MPGATTG-------------------------------------- 374
                          +P  +T                                       
Sbjct: 306 SAFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDS 365

Query: 375 ---RRLLDEG-VPSWVSEGKRRLMQAGPA--GLKPDVVVAQDGSGKYKTISEALVDVPKK 434
              R L +EG  P WV+   RRL+   P   G+K +VVVA+DGSGK KTI++AL  VP K
Sbjct: 366 LKDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMK 425

Query: 435 SNKTFVIYVKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFI-DGVQTFKTATFST 494
           + K FVI++K G+YKE V V K + H+   GDGPTKTV+TG + F+ D V T++TA+ + 
Sbjct: 426 NTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAV 485

Query: 495 IGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCS 554
            G  F AKD+GF N+AGAA+HQAVALRV +D A+F+NC M+GYQDTLY H HRQFYR+C 
Sbjct: 486 NGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCR 545

Query: 555 ISGTIDFVFGNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQSCHFMGDPA 569
           +SGTIDFVFG+A AVFQNC+ ++R+PM++QQCIVTAQGR +RRE T +V  +    GD +
Sbjct: 546 VSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDAS 605

The following BLAST results are available for this feature:

BLAST of Spg024787 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004150503.1	5.9e-273	81.60	probable pectinesterase/pectinesterase inhibitor 21 [Cucumis sativus] >KGN47423....	[more]
XP_008458409.1	1.1e-271	81.04	PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo]	[more]
XP_038875843.1	6.1e-270	80.17	probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida]	[more]
XP_023549205.1	5.4e-266	78.13	probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo]	[more]
XP_023006255.1	5.9e-265	75.54	probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita maxima]	[more]

BLAST of Spg024787 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GX86	4.4e-186	54.05	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3...	[more]
Q3E8Z8	1.9e-181	54.23	Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3...	[more]
Q9FJ21	6.2e-164	49.65	Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3...	[more]
Q8GXA1	6.4e-153	47.84	Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3...	[more]
Q42920	5.1e-134	51.11	Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1	[more]

BLAST of Spg024787 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KD24	2.9e-273	81.60	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1	[more]
A0A1S3C7B6	5.4e-272	81.04	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1	[more]
A0A6J1KZN0	2.9e-265	75.54	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499042 PE=3 SV=1	[more]
A0A6J1H4H8	4.6e-263	77.43	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1	[more]
A0A6J1KJ31	2.1e-260	76.75	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111494433 PE=3 SV=1	[more]

BLAST of Spg024787 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G05610.1	3.1e-187	54.05	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G27870.1	1.4e-182	54.23	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G49180.1	4.4e-165	49.65	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G06830.1	4.5e-154	47.84	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G15980.1	3.0e-129	39.12	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 60..212 e-value: 2.2E-38 score: 143.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 64..212 e-value: 6.7E-28 score: 97.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 60..216 e-value: 4.9E-24 score: 82.6
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 257..572 e-value: 5.2E-117 score: 392.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 59..217 e-value: 3.8E-34 score: 119.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 63..216
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 262..558 e-value: 7.3E-138 score: 458.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 37..57
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 40..56
None	No IPR available	PANTHER	PTHR31707:SF337	PECTINESTERASE	coord: 13..572
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 13..572
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 67..218 e-value: 9.93357E-48 score: 161.837
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 406..415
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 287..306
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 259..570

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg024787.1	Spg024787.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity