Spg024163 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg024163
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRNase H domain-containing protein
Locationscaffold4: 19940489 .. 19942864 (-)
RNA-Seq ExpressionSpg024163
SyntenySpg024163
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCCATCCAGTTTGAAGAGCCAGTCGAGTCACCCTTTATGGAGGCCGCCAGACCGTGGATGGTGGAAGCTCAACTCCGATGCCGCCTGGAGCAATTCCGCAAGGAGAGGAGGCGTTGGATGGGTGCTTCGTGAGTCCACCGGATCTTTAGTTGGAGCAGGTTGCAGTCAAATTACCCGGAGCTGGCCAATCAAGGCCCTTGAAGGCGAAGCTATTCGCGTTGGCTTGAGCGCGTTTCTTCGTGGGACGAGTGACGGCCGCCTCAGACCTCCGTTAAAGGTCGAATCAGATGCCCTCGGTGTCGTGAAAGTGTTAGCGGGAGAGGAAGAAGATCTTTCGGAATCAGGCCTGATCTTCGCTGTGATTTTAGAGATGTCTAGGGGAGAAAGAGTCGATTTTGAGTTGTGTAGTAGAAGTACCAATGTTTTGGCCCATCAGCTTGCGCATGCAGCAACTGAATGGGGGGATTTTTCTATGTTTTTTGGCTCTTCTTCCCCTGTTCTAGAAGATAGAGAGGTTGGTAGAGATGAGAGTATCCCTCATTGGTTTGTTTCTTTATTATCTGTGGACGTTGGTAGATCTAACGAACTACTAAACCATATTATCTCATCCAAATTTATTTAATGGAGTACTACATCAATATTCCACATTCAGCATTAAGGACATAGACTAAGGGTAACAATAATTCAAAATCTAAAAACGTAATTGAAAGTTTTTAAAGTCCATAAACGAAGACTAAGAAAACATAATGTCAAGGGGCAGTTGTATAGATGGCAAATTGAACCAAATTAATAGCAAATATAGCACAGTTTCAAACTAATTGCAAATATAGCAAAATTAATTGTACAAGACCAAAAAGCTGTTGTCGGATTCTATCCGTGTCTATCACTAATAGACTCTGATTATTCAAAGTATATCAATGTCTATCGCTGATAGACTTCGATTAATCAAAGTCTATTAGTGATAGTCACTGATAGACACTGATAGACATTGATAGATTTTGATTAACCAGAGTTTATCAGTGATAGACATTGATAGACTCTGAGTAATCGGAGTCTATCAGTAGACTGATAGATTCTGATTAATCAGAGTCTATCGCTGATAAACTCTGATAATCGAAGTCTATCAGATAGACTCCGATAGATTTTGCTATATTTGTAATTTTTTTTATATGGTGCTATATTTGCTATTAATTTGGTCCAATTTGCCACCCACTGCAGTTTTCCTAATGTTAAAGTATATATACAAAAGTTATAATCTAATCCATACTGTATAAATGTAGGCAACACGGATATGATTTATTTATATATTCTTACTAAAAGAAACTTTTGAGATTATTTAGTGAACTTTTTGTCTTTTAAAATGATTTTAGTTACAACCTTTTTTACAAAATTACTATATTACATATATTTTATAAAACCAAGGTATAGGATGCAATATAATAAATTTTCATCTTTTAACTTTCCCACACTAATAAAATTTGGATAATGTGACTGGACGACGCCGTTTGCCTGATATAAGTGTTGATTCCGAGGTTTGAACAAGGGGTCCCACCCTCACATTGGCCTGAGAGGGACTTTGTTTAATGGTTGAACCATAAACAAATTGTTCATTAGAGGACTAGTGGACTTAAGGAACAAAGATGTGCTATAGGGGTAAAACGGACTTTTGACCTGGCTATAGTTACGAATGACCTGTGAAGGATTGACTTACCATCATATTGTTCCAAAGTTTATTCCTTAAGCTAGAAAGGGGACCTAACGAACCTACAAATCATGGGCTCCAACGATTCGAGGTTTAACTGGCCAAACTTTTTTAACCTAGTTAACTAACATTCATTAACTACCGGGTCACTCCACTATAGCCTAGTAGTTGCACTCCCCTCACTGTAGATATATTTATGTCCACTCGATATAACCATGATTAGTAAGTCAACCCTTCACAGGTCGCTCGTAACTATAGCTAGGTCAAAAGTCCGTTTTACCCCTATAGCACTTCTTGTTTCCTTAAGTCCACTGGTCCTCTAATGAACAATATTAGTTTGTGATCAAACCACTAAACAGAGTCCCTCTCGGGCGAATGAGAGGGTGGGCCCTTTGTTCAAGCCTCGGAGTCAGGACTTAAGGAAACAACCTTTCTGCTATCCCTAATATTGGTAGGAGTGAATTCCTTCTTGCAATACTATGTCCCCAGCTATCTATCTGAAGGAGTGAAGAACACTTGAGCGGAAGTGTTAGCGGATCTTAGGCACTAATTTTCATTAATTTCGTTGATTAAAAGGATGTTGAAACCGAGAAAACAGATCAAGAACAACTAACCTCTTGGAGCTCCGAAATCGTTGATTCTCTTGGAAGTTTCTCCACACGAACTTGA

mRNA sequence

ATGGAGCCATCCAGTTTGAAGAGCCAGTCGAGTCACCCTTTATGGAGGCCGCCAGACCGTGGATGGTGGAAGCTCAACTCCGATGCCGCCTGGAGCAATTCCGCAAGGAGAGGAGGCGTTGGATGGGTGCTTCGTGAGTCCACCGGATCTTTAGTTGGAGCAGGTTGCAGTCAAATTACCCGGAGCTGGCCAATCAAGGCCCTTGAAGGCGAAGCTATTCGCGTTGGCTTGAGCGCGTTTCTTCGTGGGACGAGTGACGGCCGCCTCAGACCTCCGTTAAAGGTCGAATCAGATGCCCTCGGTGTCGTGAAAGTGTTAGCGGGAGAGGAAGAAGATCTTTCGGAATCAGGCCTGATCTTCGCTGTGATTTTAGAGATGTCTAGGGGAGAAAGAGTCGATTTTGAGTTGTGTAGTAGAAGTACCAATGTTTTGGCCCATCAGCTTGCGCATGCAGCAACTGAATGGGGGGATTTTTCTATGTTTTTTGGCTCTTCTTCCCCTGTTCTAGAAGATAGAGAGGATGTTGAAACCGAGAAAACAGATCAAGAACAACTAACCTCTTGGAGCTCCGAAATCGTTGATTCTCTTGGAAGTTTCTCCACACGAACTTGA

Coding sequence (CDS)

ATGGAGCCATCCAGTTTGAAGAGCCAGTCGAGTCACCCTTTATGGAGGCCGCCAGACCGTGGATGGTGGAAGCTCAACTCCGATGCCGCCTGGAGCAATTCCGCAAGGAGAGGAGGCGTTGGATGGGTGCTTCGTGAGTCCACCGGATCTTTAGTTGGAGCAGGTTGCAGTCAAATTACCCGGAGCTGGCCAATCAAGGCCCTTGAAGGCGAAGCTATTCGCGTTGGCTTGAGCGCGTTTCTTCGTGGGACGAGTGACGGCCGCCTCAGACCTCCGTTAAAGGTCGAATCAGATGCCCTCGGTGTCGTGAAAGTGTTAGCGGGAGAGGAAGAAGATCTTTCGGAATCAGGCCTGATCTTCGCTGTGATTTTAGAGATGTCTAGGGGAGAAAGAGTCGATTTTGAGTTGTGTAGTAGAAGTACCAATGTTTTGGCCCATCAGCTTGCGCATGCAGCAACTGAATGGGGGGATTTTTCTATGTTTTTTGGCTCTTCTTCCCCTGTTCTAGAAGATAGAGAGGATGTTGAAACCGAGAAAACAGATCAAGAACAACTAACCTCTTGGAGCTCCGAAATCGTTGATTCTCTTGGAAGTTTCTCCACACGAACTTGA

Protein sequence

MEPSSLKSQSSHPLWRPPDRGWWKLNSDAAWSNSARRGGVGWVLRESTGSLVGAGCSQITRSWPIKALEGEAIRVGLSAFLRGTSDGRLRPPLKVESDALGVVKVLAGEEEDLSESGLIFAVILEMSRGERVDFELCSRSTNVLAHQLAHAATEWGDFSMFFGSSSPVLEDREDVETEKTDQEQLTSWSSEIVDSLGSFSTRT
Homology
BLAST of Spg024163 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 87.4 bits (215), Expect = 1.5e-13
Identity = 54/140 (38.57%), Postives = 76/140 (54.29%), Query Frame = 0

Query: 15  WRPPDRGWWKLNSDAAWSNSARRGGVGWVLRESTGSLVGAGCSQITRSWPIKALEGEAIR 74
           W PP    W LN+DA+WS+S  RGG+GW++R   G +V AG   +     +K LE  AI 
Sbjct: 71  WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAIL 130

Query: 75  VGLSAFLRGTSDGRLRPPLKVESDALGVVKVLAGEEEDLSESGLIFAVILEMSRG-ERVD 134
            GL      T+ G LR PL +E+D+  V  +L  + EDL+++G +   IL +    E + 
Sbjct: 131 EGLRNL---TNLGVLR-PLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILA 190

Query: 135 FELCSRSTNVLAHQLAHAAT 154
           F    R TN  AH LA  A+
Sbjct: 191 FAKVGRETNGCAHSLAQRAS 206

BLAST of Spg024163 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 78.6 bits (192), Expect = 7.1e-11
Identity = 51/145 (35.17%), Postives = 70/145 (48.28%), Query Frame = 0

Query: 15  WRPPDRGWWKLNSDAAWSNSARRGGVGWVLRESTGSLVGAGCSQITRSWPIKALEGEAIR 74
           W+PP    WKLN+DAAW      GG+GW+LR+  G ++ A C  I     I  LE  AI 
Sbjct: 79  WKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAIC 138

Query: 75  VGLSAF----LRGTSDGRLRPPLKVESDALGVVKVLAGEEEDLSESGLIFAVILEMSRGE 134
            GL A      R       R P+ +ESD+L  + +L  + +D +E   +   I +M    
Sbjct: 139 EGLRAIRQEHCRPIQQEHCR-PIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDM 198

Query: 135 R-VDFELCSRSTNVLAHQLAHAATE 155
           + V     SR  N +AH LA  A E
Sbjct: 199 KIVSMRHISREANKVAHDLARRAME 222

BLAST of Spg024163 vs. NCBI nr
Match: CAB4273082.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 78.2 bits (191), Expect = 9.2e-11
Identity = 48/136 (35.29%), Postives = 74/136 (54.41%), Query Frame = 0

Query: 15   WRPPDRGWWKLNSDAAWSNSARRGGVGWVLRESTGSLVGAGCSQITRSWPIKALEGEAIR 74
            WR P  G  K+N DAAW +  + GGVGWV+R+S G +V AG     R     A+E EAIR
Sbjct: 1147 WRKPPYGVLKVNCDAAWVSQTQMGGVGWVVRDSDGWMVQAGGKGDMRGGSALAMEAEAIR 1206

Query: 75   VGLSAFLRGTSDGRLRPPLKVESDALGVVKVLAGE-EEDLSESGLIFAVILEMSRGERVD 134
              L A ++          L+VESD+L V++++ GE   D++   ++F +   + + +   
Sbjct: 1207 EALVACVQSGLS-----KLEVESDSLQVIRMIGGEWNMDIAVDTIVFDIKQLVGQFQHCV 1266

Query: 135  FELCSRSTNVLAHQLA 150
            F    R+ N  AH++A
Sbjct: 1267 FLYTPRNCNKAAHKIA 1277

BLAST of Spg024163 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 77.8 bits (190), Expect = 1.2e-10
Identity = 49/141 (34.75%), Postives = 70/141 (49.65%), Query Frame = 0

Query: 15  WRPPDRGWWKLNSDAAWSNSARRGGVGWVLRESTGSLVGAGCSQITRSWPIKALEGEAIR 74
           W+PP    WKLN++AAW      GG+GW+LR+  G ++ A C  I     I  LE  AI 
Sbjct: 79  WKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAIC 138

Query: 75  VGLSAFLRGTSDGRLRPPLKVESDALGVVKVLAGEEEDLSESGLIFAVILEMSRG-ERVD 134
            GL A  +         P+ +ESD+L  + +L  + +D +E   +   I +M +  E V 
Sbjct: 139 EGLRAIRQEHC-----RPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVS 198

Query: 135 FELCSRSTNVLAHQLAHAATE 155
               SR  N +AH LA  A E
Sbjct: 199 MRHISREANKVAHGLARRAME 214

BLAST of Spg024163 vs. NCBI nr
Match: BBH00303.1 (Ribonuclease H-like superfamily protein [Prunus dulcis])

HSP 1 Score: 76.6 bits (187), Expect = 2.7e-10
Identity = 53/145 (36.55%), Postives = 67/145 (46.21%), Query Frame = 0

Query: 13  PLWRPPDRGWWKLNSDAAWSNSARRGGVGWVLRESTGSLVGAGCSQITRSWPIKALEGEA 72
           P W  P  G  KLN D AW     RGGVGWVLR+  G  + AG     R       E EA
Sbjct: 440 PGWSSPPPGLVKLNCDGAWVAQTGRGGVGWVLRDGIGCFISAGGCGDMRCTSALMAEAEA 499

Query: 73  IRVGLSAFLRGTSDGRLRPPLKVESDALGVVKVLAGEE-EDLSESGLIFAVILEMSRGER 132
           +R  L+  L    D      + VESD+L ++K+L  E+  DL   G++F V     R   
Sbjct: 500 VREALTWCLELGFD-----KVAVESDSLSLIKMLNKEDVVDLEIEGILFDVHCLSHRAHH 559

Query: 133 VDFELCSRSTNVLAHQLAHAATEWG 157
           V+F    R  N  AH +A  A   G
Sbjct: 560 VEFLYAPRKCNQAAHNVAAYAFRIG 579

BLAST of Spg024163 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 7.3e-14
Identity = 54/140 (38.57%), Postives = 76/140 (54.29%), Query Frame = 0

Query: 15  WRPPDRGWWKLNSDAAWSNSARRGGVGWVLRESTGSLVGAGCSQITRSWPIKALEGEAIR 74
           W PP    W LN+DA+WS+S  RGG+GW++R   G +V AG   +     +K LE  AI 
Sbjct: 71  WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAIL 130

Query: 75  VGLSAFLRGTSDGRLRPPLKVESDALGVVKVLAGEEEDLSESGLIFAVILEMSRG-ERVD 134
            GL      T+ G LR PL +E+D+  V  +L  + EDL+++G +   IL +    E + 
Sbjct: 131 EGLRNL---TNLGVLR-PLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILA 190

Query: 135 FELCSRSTNVLAHQLAHAAT 154
           F    R TN  AH LA  A+
Sbjct: 191 FAKVGRETNGCAHSLAQRAS 206

BLAST of Spg024163 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 3.4e-11
Identity = 51/145 (35.17%), Postives = 70/145 (48.28%), Query Frame = 0

Query: 15  WRPPDRGWWKLNSDAAWSNSARRGGVGWVLRESTGSLVGAGCSQITRSWPIKALEGEAIR 74
           W+PP    WKLN+DAAW      GG+GW+LR+  G ++ A C  I     I  LE  AI 
Sbjct: 79  WKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAIC 138

Query: 75  VGLSAF----LRGTSDGRLRPPLKVESDALGVVKVLAGEEEDLSESGLIFAVILEMSRGE 134
            GL A      R       R P+ +ESD+L  + +L  + +D +E   +   I +M    
Sbjct: 139 EGLRAIRQEHCRPIQQEHCR-PIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDM 198

Query: 135 R-VDFELCSRSTNVLAHQLAHAATE 155
           + V     SR  N +AH LA  A E
Sbjct: 199 KIVSMRHISREANKVAHDLARRAME 222

BLAST of Spg024163 vs. ExPASy TrEMBL
Match: A0A6J5UAY2 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS20161 PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 4.5e-11
Identity = 48/136 (35.29%), Postives = 74/136 (54.41%), Query Frame = 0

Query: 15   WRPPDRGWWKLNSDAAWSNSARRGGVGWVLRESTGSLVGAGCSQITRSWPIKALEGEAIR 74
            WR P  G  K+N DAAW +  + GGVGWV+R+S G +V AG     R     A+E EAIR
Sbjct: 1147 WRKPPYGVLKVNCDAAWVSQTQMGGVGWVVRDSDGWMVQAGGKGDMRGGSALAMEAEAIR 1206

Query: 75   VGLSAFLRGTSDGRLRPPLKVESDALGVVKVLAGE-EEDLSESGLIFAVILEMSRGERVD 134
              L A ++          L+VESD+L V++++ GE   D++   ++F +   + + +   
Sbjct: 1207 EALVACVQSGLS-----KLEVESDSLQVIRMIGGEWNMDIAVDTIVFDIKQLVGQFQHCV 1266

Query: 135  FELCSRSTNVLAHQLA 150
            F    R+ N  AH++A
Sbjct: 1267 FLYTPRNCNKAAHKIA 1277

BLAST of Spg024163 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 5.8e-11
Identity = 49/141 (34.75%), Postives = 70/141 (49.65%), Query Frame = 0

Query: 15  WRPPDRGWWKLNSDAAWSNSARRGGVGWVLRESTGSLVGAGCSQITRSWPIKALEGEAIR 74
           W+PP    WKLN++AAW      GG+GW+LR+  G ++ A C  I     I  LE  AI 
Sbjct: 79  WKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAIC 138

Query: 75  VGLSAFLRGTSDGRLRPPLKVESDALGVVKVLAGEEEDLSESGLIFAVILEMSRG-ERVD 134
            GL A  +         P+ +ESD+L  + +L  + +D +E   +   I +M +  E V 
Sbjct: 139 EGLRAIRQEHC-----RPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVS 198

Query: 135 FELCSRSTNVLAHQLAHAATE 155
               SR  N +AH LA  A E
Sbjct: 199 MRHISREANKVAHGLARRAME 214

BLAST of Spg024163 vs. ExPASy TrEMBL
Match: A0A4Y1R838 (Ribonuclease H-like superfamily protein OS=Prunus dulcis OX=3755 GN=Prudu_010255 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.3e-10
Identity = 53/145 (36.55%), Postives = 67/145 (46.21%), Query Frame = 0

Query: 13  PLWRPPDRGWWKLNSDAAWSNSARRGGVGWVLRESTGSLVGAGCSQITRSWPIKALEGEA 72
           P W  P  G  KLN D AW     RGGVGWVLR+  G  + AG     R       E EA
Sbjct: 440 PGWSSPPPGLVKLNCDGAWVAQTGRGGVGWVLRDGIGCFISAGGCGDMRCTSALMAEAEA 499

Query: 73  IRVGLSAFLRGTSDGRLRPPLKVESDALGVVKVLAGEE-EDLSESGLIFAVILEMSRGER 132
           +R  L+  L    D      + VESD+L ++K+L  E+  DL   G++F V     R   
Sbjct: 500 VREALTWCLELGFD-----KVAVESDSLSLIKMLNKEDVVDLEIEGILFDVHCLSHRAHH 559

Query: 133 VDFELCSRSTNVLAHQLAHAATEWG 157
           V+F    R  N  AH +A  A   G
Sbjct: 560 VEFLYAPRKCNQAAHNVAAYAFRIG 579

BLAST of Spg024163 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 58.5 bits (140), Expect = 7.0e-09
Identity = 41/142 (28.87%), Postives = 66/142 (46.48%), Query Frame = 0

Query: 8   SQSSHPLWRPPDRGWWKLNSDAAWSNSARRGGVGWVLRESTGSLVGAGCSQITRSWPIKA 67
           ++SS   WRPP   W K N+DA W+    R G+GWVLR   G +   G   + +   +  
Sbjct: 413 NRSSCGRWRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLE 472

Query: 68  LEGEAIRVGLSAFLRGTSDGRLRPPLKVESDALGVVKVLAGEEEDLSESGLIFAVILEMS 127
            E EA+R  + +  R   +  +      ESD+  ++++L  +E   S    I  +   +S
Sbjct: 473 AELEAMRWAVLSLSRFQYNYVI-----FESDSQVLIEILNNDEIWPSLKPTIQDLQRLLS 532

Query: 128 RGERVDFELCSRSTNVLAHQLA 150
           +   V F    R  N LA ++A
Sbjct: 533 QFTEVKFVFIPREGNTLAERVA 549

BLAST of Spg024163 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 7.8e-08
Identity = 36/144 (25.00%), Postives = 64/144 (44.44%), Query Frame = 0

Query: 15  WRPPDRGWWKLNSDAAWSNSARRGGVGWVLRESTGSLVGAGCSQITRSWPIKALEGEAIR 74
           W+ P   W K N+DA W     R G+GW+LR  +G ++  G   + R+  +   E EA+R
Sbjct: 137 WKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEALR 196

Query: 75  VGLSAFLRGTSDGRLRPPLKVESDALGVVKVLAGEEEDLSESGLIFAVILEMSRGERVDF 134
             +    R          +  ESDA  +V +L  ++   +    +  +   +   E V F
Sbjct: 197 WAVLTMSRFN-----YKRIIFESDAQALVNLLNSDDFWPTLQPALEDIQQLLHHFEEVKF 256

Query: 135 ELCSRSTNVLAHQLAHAATEWGDF 159
           E   R  N +A ++A  +  + ++
Sbjct: 257 EFTPRGGNKVADRIARESISFSNY 275

BLAST of Spg024163 vs. TAIR 10
Match: AT5G19270.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G03566.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 48.1 bits (113), Expect = 9.5e-06
Identity = 39/157 (24.84%), Postives = 60/157 (38.22%), Query Frame = 0

Query: 15  WRPPDRGWWKLNSDAAWSNSARRGGVGWVLRESTGSLVGAGCSQITRSWPIKALEGEAIR 74
           W PPD G  K N +A W N+    GV W+ R+  G  +       TRS          I 
Sbjct: 61  WTPPDFGIVKCNVNAHWGNADMMSGVAWIARDVNGLALYHARDAFTRS-------SNRIM 120

Query: 75  VGLSAFL---RGTSDGRLRPPLKVESDALGVVKVLAGEEEDLSESGLIFAVILEMSRGER 134
             L   L   +   D RL+  + + SD    +  L    +    +  +  + +       
Sbjct: 121 AELRCILWVVKSLRDLRLQNVI-ISSDCQAAIAALYNPRDWPRYAMWLDTIFIACQSFPF 180

Query: 135 VDFELCSRSTNVLAHQLAHAATEWGDFSMFFGSSSPV 169
           + FEL S   N +A  +A + T  G F+ +     PV
Sbjct: 181 IIFELVSCPANSIARDIAESVTRDGRFNPYLSEGGPV 209

BLAST of Spg024163 vs. TAIR 10
Match: AT1G27870.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.2e-05
Identity = 20/50 (40.00%), Postives = 31/50 (62.00%), Query Frame = 0

Query: 6  LKSQSSHPLWRPPDRGWWKLNSDAAWSNSARRGGVGWVLRESTGSLVGAG 56
          ++ + +H  WR P+RGW K N D ++ N   +   GWV+R+S GS + AG
Sbjct: 43 VREEDNHDRWRRPERGWIKCNFDGSFVNGDVKSKAGWVVRDSNGSYLLAG 92

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155262.11.5e-1338.57uncharacterized protein LOC111022403 [Momordica charantia][more]
XP_022156777.17.1e-1135.17uncharacterized protein LOC111023608 [Momordica charantia][more]
CAB4273082.19.2e-1135.29unnamed protein product [Prunus armeniaca][more]
XP_022143535.11.2e-1034.75uncharacterized protein LOC111013412 [Momordica charantia][more]
BBH00303.12.7e-1036.55Ribonuclease H-like superfamily protein [Prunus dulcis][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DNV97.3e-1438.57uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1DSV13.4e-1135.17uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J5UAY24.5e-1135.29Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=... [more]
A0A6J1CP265.8e-1134.75uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A4Y1R8381.3e-1036.55Ribonuclease H-like superfamily protein OS=Prunus dulcis OX=3755 GN=Prudu_010255... [more]
Match NameE-valueIdentityDescription
AT4G29090.17.0e-0928.87Ribonuclease H-like superfamily protein [more]
AT2G34320.17.8e-0825.00Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G19270.19.5e-0624.84unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G27870.11.2e-0540.00Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 26..152
e-value: 2.3E-16
score: 59.8
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 23..156
e-value: 7.3E-7
score: 31.2
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 12..154
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 12..154
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 25..150
e-value: 3.50902E-14
score: 64.2576
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 21..154

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg024163.1Spg024163.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity