Spg024128 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg024128
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCalmodulin-related, putative isoform 1
Locationscaffold4: 19314838 .. 19317271 (-)
RNA-Seq ExpressionSpg024128
SyntenySpg024128
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGAACAACGCTACTCTCCCCTACAAAAGCAACCCTCCACACCCAACATCCGTACCTCCCTCATTCCCATACTCCCGTCGTCCCCCCTTTCCTAACGATTTACAAGATTATTTCCCTTTTTTCCCCTTCTTAACATACCTATTCATAATGGGGGCATTACAGTTTCATGCCTACAGGGAAGTTGAGTCCTGCCTAAAATATATGAAAAGGGAAAATGTCTATTCTGCACGTATTTTATGTAGAAATGTGAATTTCATCTATTTCAACTCGTTCTCATTGGTGTAGTTTGAAAAGTTTTAAAATCTATTTCAGGGAAAGAACAAAGGCTCAAAAGCAAAGAGAAAAGCTTCTGTCGAGAAATCAAATAACTTCGACGATGAAGATGATGATGATGCCTTACGACAGGTAGATTCATAACATTCCATCAATAATTTGTAATACAAGACAGCACAAACAGGCTAACTATATCATGACTTCCCTAGCAGGCAATCGAACTTTCACTACGAGATTCTGGTGAAAATTCTGATGCTCAGGTGCGAGGTCTGTTTGAAAATGTTAGAAGGAAAAATGTGAAGAATCAGGAAGTGAGAGGAGGGATGAAGAGAAAAGGATCGGTAGCTTTTTAACTCAACGTTATAGCCTTGGTGATCCTTTATATTTTTTTTCTCTTAATGCTTTGTCTTCACATCTGATCTGCAGTTAACTAGTCGGATGCAGATGAATGAAGATGAACTTATCATGAACTTCTATTGCTTTGATGGTAATTTATATATTGTTATAAATTAAAACACTTTAAACTAATTTTTGCTACTGAGCTGGTGAATTTGACTAATAGAGTCATGGAAAGGGAGCATTGTTGTTCGTGACTTGAGAAGAGTGGCAGCTGCTTATGATTTTATTTGGTCTGACCAAGAATTAATGGATATGATTGATTGTTTTGACAGTGATGGAGATGGGAAGGTTAGTTTCTTGGATTCATGCTCTTCTTTCATCTTCCTATTCCATTCTTTTGAATCGTAATATTAAAATGGTTTTGAGTCTTAAGATATATGTTTTCAATTTTGTCATTCAAGTTTCAAAGTTCTTAATAATACCATCAAACTTACCCGCTATTTTAAAAATGTTCTTAGTTCTCCCTTTACTTAACAACACCAACAGTGATAAGACATGATACATTATATTTAGTTCACTTGTTTAGAAAAAGACATCTTGACATCAGTGACAATGATGAAGAGAGTGTAACCTTTTACATTGTGTTTACAAACTTCTTCAAGACATTTTTTAAAACATTTGATGCATCTTCTCTGCTTATTTTTTTTTTTCAAAACAGACAATACTTATCAAATATCTTTTAGGTGGTGGTTCTGATATTATGGCAATTGCTTCTAGAAAAGATCAAAATGTTAATGGTAACCTACCAAAGAAGTAAGAATCATAAACTACCTAAATTTCAGCATCATCAGAAGGCTACAGAAAGTCTTTGAAGGTTTATATCTCTAACTACTGGAATATTTTTCGAAAGGAAATTTTTTTTGACCAGTTACCTATATGTATATAGCCTGTTGTTTCAGCCAGTAAATATAATTACTTTTATCTACCATCTTGACGAACATATCTTGCTTGAATCCAAGCTTTGATCATCCTTTCCAAGATATATCTGACCTTCATTTTCCTTTTCCATAACAGTTGAATCTGGATGATTTCCGGAGAATTGCTGGTCGATGTAACATGATAAAAGAGTCCATCACTTGATTGACTTGGACAATGACAGACAGACTGGTGCACCAATGGGCAAGTATGGAGGAGGATCTTGACAAGAATCGAAGAGGATTGTGGTACCAATTGCATCCCTCACTCTCTCTCTCTGCATTTTTGTAGAACAATCAAATTGAGATATGCTCTTTGTCCAACCAATCCGAAACATATTATGAGGTTTGTCGCTCGGAGCATCAAAACTTTCTTGTTGGAAGAAAAAGCTCTGAAGTTCATCATAAGATGACGACCATAGTGGAGCAACTAATCAACTCAAGTCAAGAACCGATCTTCATGTTTTTGTGGATTTCTGAAGTCTTGTAGCTGTAGTGCATTCAAACTTCTCGTCGTGAATGTGAGGTTCCGACCCCATGTGTTTCAAGCTTTGAGATCTGTCTTTGAAGTTTGAAACTGAGGGGAAAGATGTATTGGTATTGTCATGTGTGGGGTTGATTGTGATATTCCTGCTATACTAGTATGGTTAATTTGGTTGAAGGGCATTTGTTTATTATTCTGCTCAAAACATTGCGGTTACAAAAATTGTATGTTTTGGGGATGCTGAATCTGAAAATGCAAGATCATATATTTCAGTTCAATTGGATAGAGTATCGGCAACTTGCTTGATTATGTAGGTGGTCACCTCAAGTCTGAACCTATTCTAAATTAACCAGCCTATGAC

mRNA sequence

ATGCCGAACAACGCTACTCTCCCCTACAAAAGCAACCCTCCACACCCAACATCCGGAAAGAACAAAGGCTCAAAAGCAAAGAGAAAAGCTTCTGTCGAGAAATCAAATAACTTCGACGATGAAGATGATGATGATGCCTTACGACAGGCAATCGAACTTTCACTACGAGATTCTGGTGAAAATTCTGATGCTCAGGTGCGAGGTCTGTTTGAAAATGTTAGAAGGAAAAATGTGAAGAATCAGGAAGTGAGAGGAGGGATGAAGAGAAAAGGATCGTTAACTAGTCGGATGCAGATGAATGAAGATGAACTTATCATGAACTTCTATTGCTTTGATGAGTCATGGAAAGGGAGCATTGTTGTTCGTGACTTGAGAAGAGTGGCAGCTGCTTATGATTTTATTTGGTCTGACCAAGAATTAATGGATATGATTGATTGTTTTGACAGTGATGGAGATGGGAAGTTGAATCTGGATGATTTCCGGAGAATTGCTGGTCGATGTAACATGATAAAAGAGTCCATCACTTGA

Coding sequence (CDS)

ATGCCGAACAACGCTACTCTCCCCTACAAAAGCAACCCTCCACACCCAACATCCGGAAAGAACAAAGGCTCAAAAGCAAAGAGAAAAGCTTCTGTCGAGAAATCAAATAACTTCGACGATGAAGATGATGATGATGCCTTACGACAGGCAATCGAACTTTCACTACGAGATTCTGGTGAAAATTCTGATGCTCAGGTGCGAGGTCTGTTTGAAAATGTTAGAAGGAAAAATGTGAAGAATCAGGAAGTGAGAGGAGGGATGAAGAGAAAAGGATCGTTAACTAGTCGGATGCAGATGAATGAAGATGAACTTATCATGAACTTCTATTGCTTTGATGAGTCATGGAAAGGGAGCATTGTTGTTCGTGACTTGAGAAGAGTGGCAGCTGCTTATGATTTTATTTGGTCTGACCAAGAATTAATGGATATGATTGATTGTTTTGACAGTGATGGAGATGGGAAGTTGAATCTGGATGATTTCCGGAGAATTGCTGGTCGATGTAACATGATAAAAGAGTCCATCACTTGA

Protein sequence

MPNNATLPYKSNPPHPTSGKNKGSKAKRKASVEKSNNFDDEDDDDALRQAIELSLRDSGENSDAQVRGLFENVRRKNVKNQEVRGGMKRKGSLTSRMQMNEDELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSDQELMDMIDCFDSDGDGKLNLDDFRRIAGRCNMIKESIT
Homology
BLAST of Spg024128 vs. NCBI nr
Match: XP_004140495.1 (uncharacterized protein LOC101206207 [Cucumis sativus] >KGN46547.1 hypothetical protein Csa_005249 [Cucumis sativus])

HSP 1 Score: 266.2 bits (679), Expect = 2.1e-67
Identity = 132/159 (83.02%), Postives = 145/159 (91.19%), Query Frame = 0

Query: 17  TSGKNKGSKAKRKASVEKSNNFDDEDDDDALRQAIELSLRDSGENSDAQVRGLFENVRRK 76
           T GKN+GS+ KRK SV+KSNNF+ EDDDDAL+QAI+LSL+DSGENSDAQV+G FENVRRK
Sbjct: 128 TKGKNRGSETKRKVSVKKSNNFNHEDDDDALQQAIKLSLQDSGENSDAQVQGSFENVRRK 187

Query: 77  NVKNQEVRGGMKRKGSLTSRMQMNEDELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWS 136
           N+KNQE RGGMKRKG  TSRMQMNEDELIMNFYCFDESWKG I VRDL+RVA A+DF WS
Sbjct: 188 NLKNQEARGGMKRKGLFTSRMQMNEDELIMNFYCFDESWKGGITVRDLKRVADAHDFTWS 247

Query: 137 DQELMDMIDCFDSDGDGKLNLDDFRRIAGRCNMIKESIT 176
           + EL DMIDCFD+DGDGKLNLDDFRRIAGRCNMIKESIT
Sbjct: 248 EIELRDMIDCFDNDGDGKLNLDDFRRIAGRCNMIKESIT 286

BLAST of Spg024128 vs. NCBI nr
Match: XP_038874842.1 (centrin-1-like [Benincasa hispida])

HSP 1 Score: 258.8 bits (660), Expect = 3.3e-65
Identity = 132/158 (83.54%), Postives = 143/158 (90.51%), Query Frame = 0

Query: 19  GKNKGSKAKRKASVEKSNNFDDEDDD-DALRQAIELSLRDSGENSDAQVRGLFENVRRKN 78
           GKN+GS+AKRK  V+KSNN DDEDDD DALRQAI+LSL+DSGEN DAQV+G  ENVRRKN
Sbjct: 110 GKNRGSEAKRKVPVKKSNNSDDEDDDEDALRQAIKLSLQDSGENCDAQVQGPLENVRRKN 169

Query: 79  VKNQEVRGGMKRKGSLTSRMQMNEDELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSD 138
           VKNQEVRGGMKRKG  TSRMQMN+DELIMNFYCFD+SWKG I V DL+RVAAA+DF WSD
Sbjct: 170 VKNQEVRGGMKRKGLFTSRMQMNDDELIMNFYCFDDSWKGDITVSDLKRVAAAHDFTWSD 229

Query: 139 QELMDMIDCFDSDGDGKLNLDDFRRIAGRCNMIKESIT 176
            EL DMIDCFDSDGDGKLNL+DFRRIAGRCNMIKESIT
Sbjct: 230 IELRDMIDCFDSDGDGKLNLNDFRRIAGRCNMIKESIT 267

BLAST of Spg024128 vs. NCBI nr
Match: XP_022159279.1 (uncharacterized protein LOC111025692 isoform X1 [Momordica charantia])

HSP 1 Score: 258.1 bits (658), Expect = 5.6e-65
Identity = 130/154 (84.42%), Postives = 140/154 (90.91%), Query Frame = 0

Query: 20  KNKGSKAKRKASVEKSNNFDDEDDDDALRQAIELSLRDSGENSDAQVRGLFENVRRKNVK 79
           KNKGSKAKRK SVEKSNN DDEDDD+ALRQAIELSLRDSG NSDAQV+G  ENV+RKNVK
Sbjct: 134 KNKGSKAKRKTSVEKSNNLDDEDDDNALRQAIELSLRDSGVNSDAQVQGAIENVKRKNVK 193

Query: 80  NQEVRGGMKRKGSLTSRMQMNEDELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSDQE 139
            Q++ GGMKRKGS TSRMQMNEDELI+NFY FDE WKGSI V D++RVAAA+DF WSDQE
Sbjct: 194 TQKITGGMKRKGSFTSRMQMNEDELIVNFYYFDELWKGSISVSDMKRVAAAHDFTWSDQE 253

Query: 140 LMDMIDCFDSDGDGKLNLDDFRRIAGRCNMIKES 174
           L DMIDCFDSDGDGKLNLDDFRRIAG+CNMIKES
Sbjct: 254 LKDMIDCFDSDGDGKLNLDDFRRIAGQCNMIKES 287

BLAST of Spg024128 vs. NCBI nr
Match: XP_022959491.1 (caltractin-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 249.6 bits (636), Expect = 2.0e-62
Identity = 127/157 (80.89%), Postives = 142/157 (90.45%), Query Frame = 0

Query: 19  GKNKGSKAKRKASVEKSNNFDDEDDDDALRQAIELSLRDSGENSDAQVRGLFENVRRKNV 78
           GKN+GSKAKRK+SVEKSNN +DE DDDAL+QAI+LSL+DSGE SDAQ++G FENVRRKNV
Sbjct: 128 GKNRGSKAKRKSSVEKSNNLNDE-DDDALQQAIKLSLQDSGETSDAQMQGPFENVRRKNV 187

Query: 79  KNQEVRGGMKRKGSLTSRMQMNEDELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSDQ 138
           KNQE+ GGMKRKG  TSRMQMNEDELIMNFYCFDESWKGSI V DL+RVAA +DF WS +
Sbjct: 188 KNQEMGGGMKRKGLFTSRMQMNEDELIMNFYCFDESWKGSITVSDLKRVAAVHDFTWSVK 247

Query: 139 ELMDMIDCFDSDGDGKLNLDDFRRIAGRCNMIKESIT 176
           EL DMI+CFDSDGD KL++DDFRRIA RCNMIKESIT
Sbjct: 248 ELEDMINCFDSDGDAKLSMDDFRRIASRCNMIKESIT 283

BLAST of Spg024128 vs. NCBI nr
Match: XP_023006622.1 (caltractin-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 248.4 bits (633), Expect = 4.4e-62
Identity = 126/157 (80.25%), Postives = 142/157 (90.45%), Query Frame = 0

Query: 19  GKNKGSKAKRKASVEKSNNFDDEDDDDALRQAIELSLRDSGENSDAQVRGLFENVRRKNV 78
           GKN+GSK KRK+SVEKSNN +DE DDDAL+QAI+LSL++SGE SDAQ++G FENVRRKNV
Sbjct: 128 GKNRGSKEKRKSSVEKSNNLNDE-DDDALQQAIKLSLQESGETSDAQMQGPFENVRRKNV 187

Query: 79  KNQEVRGGMKRKGSLTSRMQMNEDELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSDQ 138
           KNQE+ GGMKRKG  TSRMQMNEDELIMNFYCFDESWKGSI VRDL+RVAA +DF WS +
Sbjct: 188 KNQEMGGGMKRKGLFTSRMQMNEDELIMNFYCFDESWKGSITVRDLKRVAAVHDFTWSVK 247

Query: 139 ELMDMIDCFDSDGDGKLNLDDFRRIAGRCNMIKESIT 176
           EL DMI+CFDSDGD KL++DDFRRIA RCNMIKESIT
Sbjct: 248 ELEDMINCFDSDGDAKLSMDDFRRIASRCNMIKESIT 283

BLAST of Spg024128 vs. ExPASy Swiss-Prot
Match: Q32LE3 (Centrin-1 OS=Bos taurus OX=9913 GN=CETN1 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 7.2e-07
Identity = 27/69 (39.13%), Postives = 43/69 (62.32%), Query Frame = 0

Query: 101 EDELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSDQELMDMIDCFDSDGDGKLNLDDF 160
           ++E++  F  FD+   G I  ++L+RVA       +D+EL +MID  D DGDG++N D+F
Sbjct: 103 KEEILKAFRLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEVNEDEF 162

Query: 161 RRIAGRCNM 170
            RI  + N+
Sbjct: 163 LRIMKKTNL 171

BLAST of Spg024128 vs. ExPASy Swiss-Prot
Match: O35648 (Centrin-3 OS=Mus musculus OX=10090 GN=Cetn3 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 3.6e-06
Identity = 26/59 (44.07%), Postives = 38/59 (64.41%), Query Frame = 0

Query: 102 DELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSDQELMDMIDCFDSDGDGKLNLDDF 161
           +E++  F  FD+   G I +R+LRRVA       SD+EL  MI+ FD DGDG++N ++F
Sbjct: 101 EEILKAFKLFDDDDSGKISLRNLRRVARELGENMSDEELRAMIEEFDKDGDGEINQEEF 159

BLAST of Spg024128 vs. ExPASy Swiss-Prot
Match: P27482 (Calmodulin-like protein 3 OS=Homo sapiens OX=9606 GN=CALML3 PE=1 SV=2)

HSP 1 Score: 50.1 bits (118), Expect = 3.0e-05
Identity = 39/130 (30.00%), Postives = 64/130 (49.23%), Query Frame = 0

Query: 38  FDDEDDDDALRQAIELSLRDSGEN-SDAQVRGLFENVRRK---NVKNQEVRGGMKRKGSL 97
           FD + D     + +   +R  G+N ++A++R +   + R     V   E  G M RK   
Sbjct: 20  FDKDGDGCITTRELGTVMRSLGQNPTEAELRDMMSEIDRDGNGTVDFPEFLGMMARKMKD 79

Query: 98  TSRMQMNEDELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSDQELMDMIDCFDSDGDG 157
           T     NE+E+   F  FD+   G +   +LR V        SD+E+ +MI   D+DGDG
Sbjct: 80  TD----NEEEIREAFRVFDKDGNGFVSAAELRHVMTRLGEKLSDEEVDEMIRAADTDGDG 139

Query: 158 KLNLDDFRRI 164
           ++N ++F R+
Sbjct: 140 QVNYEEFVRV 145

BLAST of Spg024128 vs. ExPASy Swiss-Prot
Match: Q06827 (Caltractin OS=Scherffelia dubia OX=3190 PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 3.4e-04
Identity = 43/166 (25.90%), Postives = 84/166 (50.60%), Query Frame = 0

Query: 22  KGSKAKRKASVEKSNNFDDEDDDDALRQAIELSLRDSGENSDAQ-----VRGLFENVRRK 81
           K + A+R  +  +S    +E   + +R+A +L   D     DA+     +R L    +++
Sbjct: 5   KAASARRDKAKTRSAGLTEEQKQE-IREAFDLFDTDGSGTIDAKELKVAMRALGFEPKKE 64

Query: 82  NVKNQEVRGGMKRKGS-----------LTSRM--QMNEDELIMNFYCFDESWKGSIVVRD 141
            +K  ++   + + GS           +T++M  + + +E++  F  FD+   G I  ++
Sbjct: 65  EIK--KMIADIDKDGSGTIDFEEFLQMMTAKMGERDSREEIMKAFRLFDDDETGKISFKN 124

Query: 142 LRRVAAAYDFIWSDQELMDMIDCFDSDGDGKLNLDDFRRIAGRCNM 170
           L+RVA       +D+EL +MID  D DGDG++N ++F RI  + ++
Sbjct: 125 LKRVAKELGENMTDEELQEMIDEADRDGDGEVNEEEFFRIMKKTSL 167

BLAST of Spg024128 vs. ExPASy Swiss-Prot
Match: O15182 (Centrin-3 OS=Homo sapiens OX=9606 GN=CETN3 PE=1 SV=2)

HSP 1 Score: 46.6 bits (109), Expect = 3.4e-04
Identity = 47/153 (30.72%), Postives = 69/153 (45.10%), Query Frame = 0

Query: 25  KAKRKASVEKSNNFDDEDDDDALRQAIELSLRDSGENSD-----AQVRGLFENVRRKNVK 84
           K KRK   E S     E+    ++ A EL   D  E  D       +R L  +V++ +V 
Sbjct: 13  KTKRKKRRELS-----EEQKQEIKDAFELFDTDKDEAIDYHELKVAMRALGFDVKKADVL 72

Query: 85  NQEVRGGMKRKGSLTSRMQMNE------------DELIMNFYCFDESWKGSIVVRDLRRV 144
                   +  G +T     NE            +E++  F  FD+   G I +R+LRRV
Sbjct: 73  KILKDYDREATGKITFE-DFNEVVTDWILERDPHEEILKAFKLFDDDDSGKISLRNLRRV 132

Query: 145 AAAYDFIWSDQELMDMIDCFDSDGDGKLNLDDF 161
           A       SD+EL  MI+ FD DGDG++N ++F
Sbjct: 133 ARELGENMSDEELRAMIEEFDKDGDGEINQEEF 159

BLAST of Spg024128 vs. ExPASy TrEMBL
Match: A0A0A0KDV1 (EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G108450 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 1.0e-67
Identity = 132/159 (83.02%), Postives = 145/159 (91.19%), Query Frame = 0

Query: 17  TSGKNKGSKAKRKASVEKSNNFDDEDDDDALRQAIELSLRDSGENSDAQVRGLFENVRRK 76
           T GKN+GS+ KRK SV+KSNNF+ EDDDDAL+QAI+LSL+DSGENSDAQV+G FENVRRK
Sbjct: 128 TKGKNRGSETKRKVSVKKSNNFNHEDDDDALQQAIKLSLQDSGENSDAQVQGSFENVRRK 187

Query: 77  NVKNQEVRGGMKRKGSLTSRMQMNEDELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWS 136
           N+KNQE RGGMKRKG  TSRMQMNEDELIMNFYCFDESWKG I VRDL+RVA A+DF WS
Sbjct: 188 NLKNQEARGGMKRKGLFTSRMQMNEDELIMNFYCFDESWKGGITVRDLKRVADAHDFTWS 247

Query: 137 DQELMDMIDCFDSDGDGKLNLDDFRRIAGRCNMIKESIT 176
           + EL DMIDCFD+DGDGKLNLDDFRRIAGRCNMIKESIT
Sbjct: 248 EIELRDMIDCFDNDGDGKLNLDDFRRIAGRCNMIKESIT 286

BLAST of Spg024128 vs. ExPASy TrEMBL
Match: A0A6J1E1Z5 (uncharacterized protein LOC111025692 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025692 PE=4 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.7e-65
Identity = 130/154 (84.42%), Postives = 140/154 (90.91%), Query Frame = 0

Query: 20  KNKGSKAKRKASVEKSNNFDDEDDDDALRQAIELSLRDSGENSDAQVRGLFENVRRKNVK 79
           KNKGSKAKRK SVEKSNN DDEDDD+ALRQAIELSLRDSG NSDAQV+G  ENV+RKNVK
Sbjct: 134 KNKGSKAKRKTSVEKSNNLDDEDDDNALRQAIELSLRDSGVNSDAQVQGAIENVKRKNVK 193

Query: 80  NQEVRGGMKRKGSLTSRMQMNEDELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSDQE 139
            Q++ GGMKRKGS TSRMQMNEDELI+NFY FDE WKGSI V D++RVAAA+DF WSDQE
Sbjct: 194 TQKITGGMKRKGSFTSRMQMNEDELIVNFYYFDELWKGSISVSDMKRVAAAHDFTWSDQE 253

Query: 140 LMDMIDCFDSDGDGKLNLDDFRRIAGRCNMIKES 174
           L DMIDCFDSDGDGKLNLDDFRRIAG+CNMIKES
Sbjct: 254 LKDMIDCFDSDGDGKLNLDDFRRIAGQCNMIKES 287

BLAST of Spg024128 vs. ExPASy TrEMBL
Match: A0A6J1H502 (caltractin-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111460448 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 9.6e-63
Identity = 127/157 (80.89%), Postives = 142/157 (90.45%), Query Frame = 0

Query: 19  GKNKGSKAKRKASVEKSNNFDDEDDDDALRQAIELSLRDSGENSDAQVRGLFENVRRKNV 78
           GKN+GSKAKRK+SVEKSNN +DE DDDAL+QAI+LSL+DSGE SDAQ++G FENVRRKNV
Sbjct: 128 GKNRGSKAKRKSSVEKSNNLNDE-DDDALQQAIKLSLQDSGETSDAQMQGPFENVRRKNV 187

Query: 79  KNQEVRGGMKRKGSLTSRMQMNEDELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSDQ 138
           KNQE+ GGMKRKG  TSRMQMNEDELIMNFYCFDESWKGSI V DL+RVAA +DF WS +
Sbjct: 188 KNQEMGGGMKRKGLFTSRMQMNEDELIMNFYCFDESWKGSITVSDLKRVAAVHDFTWSVK 247

Query: 139 ELMDMIDCFDSDGDGKLNLDDFRRIAGRCNMIKESIT 176
           EL DMI+CFDSDGD KL++DDFRRIA RCNMIKESIT
Sbjct: 248 ELEDMINCFDSDGDAKLSMDDFRRIASRCNMIKESIT 283

BLAST of Spg024128 vs. ExPASy TrEMBL
Match: A0A6J1KWC6 (caltractin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499295 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 2.1e-62
Identity = 126/157 (80.25%), Postives = 142/157 (90.45%), Query Frame = 0

Query: 19  GKNKGSKAKRKASVEKSNNFDDEDDDDALRQAIELSLRDSGENSDAQVRGLFENVRRKNV 78
           GKN+GSK KRK+SVEKSNN +DE DDDAL+QAI+LSL++SGE SDAQ++G FENVRRKNV
Sbjct: 128 GKNRGSKEKRKSSVEKSNNLNDE-DDDALQQAIKLSLQESGETSDAQMQGPFENVRRKNV 187

Query: 79  KNQEVRGGMKRKGSLTSRMQMNEDELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSDQ 138
           KNQE+ GGMKRKG  TSRMQMNEDELIMNFYCFDESWKGSI VRDL+RVAA +DF WS +
Sbjct: 188 KNQEMGGGMKRKGLFTSRMQMNEDELIMNFYCFDESWKGSITVRDLKRVAAVHDFTWSVK 247

Query: 139 ELMDMIDCFDSDGDGKLNLDDFRRIAGRCNMIKESIT 176
           EL DMI+CFDSDGD KL++DDFRRIA RCNMIKESIT
Sbjct: 248 ELEDMINCFDSDGDAKLSMDDFRRIASRCNMIKESIT 283

BLAST of Spg024128 vs. ExPASy TrEMBL
Match: A0A6J1DY88 (uncharacterized protein LOC111025692 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025692 PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 2.8e-54
Identity = 116/146 (79.45%), Postives = 127/146 (86.99%), Query Frame = 0

Query: 20  KNKGSKAKRKASVEKSNNFDDEDDDDALRQAIELSLRDSGENSDAQVRGLFENVRRKNVK 79
           KNKGSKAKRK SVEKSNN DDEDDD+ALRQAIELSLRDSG NSDAQV+G  ENV+RKNVK
Sbjct: 134 KNKGSKAKRKTSVEKSNNLDDEDDDNALRQAIELSLRDSGVNSDAQVQGAIENVKRKNVK 193

Query: 80  NQEVRGGMKRKGSLTSRMQMNEDELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSDQE 139
            Q++ GGMKRKGS TSRMQMNEDELI+NFY FDE WKGSI V D++RVAAA+DF WSDQE
Sbjct: 194 TQKITGGMKRKGSFTSRMQMNEDELIVNFYYFDELWKGSISVSDMKRVAAAHDFTWSDQE 253

Query: 140 LMDMIDCFDSDGD--GKLNLDDFRRI 164
           L DMIDCFDSDGD  GKL+L  FR +
Sbjct: 254 LKDMIDCFDSDGDGKGKLSLCCFRTL 279

BLAST of Spg024128 vs. TAIR 10
Match: AT1G73440.1 (calmodulin-related )

HSP 1 Score: 111.7 bits (278), Expect = 6.0e-25
Identity = 68/156 (43.59%), Postives = 90/156 (57.69%), Query Frame = 0

Query: 20  KNKGSKAKRKASVEKSNNFDDE--DDDDALRQAIELSLRDSGENSDAQVRGLFENVRRKN 79
           KNK S +KRK S  K  N      +DDD L +AI LSL+ S   SD              
Sbjct: 116 KNKSSASKRKVSSRKILNTSVSLGEDDDDLDKAIALSLQGSVAGSD-------------- 175

Query: 80  VKNQEVRGGMKRKGSLTSRMQMNEDELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSD 139
              +E     K++  L S+ QM +DEL+M F  FDE  KG I +RD+ ++A  +DF W++
Sbjct: 176 ---KEAATMKKKRPELMSKTQMTQDELVMYFCQFDEGGKGFITLRDVAKMATVHDFTWTE 235

Query: 140 QELMDMIDCFDSDGDGKLNLDDFRRIAGRCNMIKES 174
           +EL DMI CFD D DGKL+LD+FR+I  RC M+K S
Sbjct: 236 EELQDMIRCFDMDKDGKLSLDEFRKIVSRCRMLKGS 254

BLAST of Spg024128 vs. TAIR 10
Match: AT4G00140.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 48.9 bits (115), Expect = 4.8e-06
Identity = 21/43 (48.84%), Postives = 29/43 (67.44%), Query Frame = 0

Query: 113 ESWKGSIVVRDLRRVAAAYDFIWSDQELMDMIDCFDSDGDGKL 156
           E  KG I  RD+ ++A  +DF W+++EL DMI  FD D DGK+
Sbjct: 91  EGGKGFITRRDVAKMATVHDFTWTEEELQDMIRSFDMDKDGKV 133

BLAST of Spg024128 vs. TAIR 10
Match: AT3G50360.1 (centrin2 )

HSP 1 Score: 42.4 bits (98), Expect = 4.5e-04
Identity = 21/66 (31.82%), Postives = 37/66 (56.06%), Query Frame = 0

Query: 101 EDELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSDQELMDMIDCFDSDGDGKLNLDDF 160
           ++EL   F   D    G I   D++R+A      ++D E+ +M++  D D DG++N+D+F
Sbjct: 98  KEELTKAFQIIDLDKNGKISPDDIKRMAKDLGENFTDAEIREMVEEADRDRDGEVNMDEF 157

Query: 161 RRIAGR 167
            R+  R
Sbjct: 158 MRMMRR 163

BLAST of Spg024128 vs. TAIR 10
Match: AT4G37010.1 (centrin 2 )

HSP 1 Score: 42.4 bits (98), Expect = 4.5e-04
Identity = 21/65 (32.31%), Postives = 36/65 (55.38%), Query Frame = 0

Query: 102 DELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSDQELMDMIDCFDSDGDGKLNLDDFR 161
           DEL   F   D    G I  RD++ +A      ++D ++ +MI+  D D DG++NL++F 
Sbjct: 99  DELSKAFKIIDHDNNGKISPRDIKMIAKELGENFTDNDIEEMIEEADRDKDGEVNLEEFM 158

Query: 162 RIAGR 167
           ++  R
Sbjct: 159 KMMKR 163

BLAST of Spg024128 vs. TAIR 10
Match: AT4G37010.2 (centrin 2 )

HSP 1 Score: 42.4 bits (98), Expect = 4.5e-04
Identity = 21/65 (32.31%), Postives = 36/65 (55.38%), Query Frame = 0

Query: 102 DELIMNFYCFDESWKGSIVVRDLRRVAAAYDFIWSDQELMDMIDCFDSDGDGKLNLDDFR 161
           DEL   F   D    G I  RD++ +A      ++D ++ +MI+  D D DG++NL++F 
Sbjct: 103 DELSKAFKIIDHDNNGKISPRDIKMIAKELGENFTDNDIEEMIEEADRDKDGEVNLEEFM 162

Query: 162 RIAGR 167
           ++  R
Sbjct: 163 KMMKR 167

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004140495.12.1e-6783.02uncharacterized protein LOC101206207 [Cucumis sativus] >KGN46547.1 hypothetical ... [more]
XP_038874842.13.3e-6583.54centrin-1-like [Benincasa hispida][more]
XP_022159279.15.6e-6584.42uncharacterized protein LOC111025692 isoform X1 [Momordica charantia][more]
XP_022959491.12.0e-6280.89caltractin-like isoform X2 [Cucurbita moschata][more]
XP_023006622.14.4e-6280.25caltractin-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q32LE37.2e-0739.13Centrin-1 OS=Bos taurus OX=9913 GN=CETN1 PE=2 SV=1[more]
O356483.6e-0644.07Centrin-3 OS=Mus musculus OX=10090 GN=Cetn3 PE=1 SV=1[more]
P274823.0e-0530.00Calmodulin-like protein 3 OS=Homo sapiens OX=9606 GN=CALML3 PE=1 SV=2[more]
Q068273.4e-0425.90Caltractin OS=Scherffelia dubia OX=3190 PE=1 SV=1[more]
O151823.4e-0430.72Centrin-3 OS=Homo sapiens OX=9606 GN=CETN3 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KDV11.0e-6783.02EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G108450 PE=... [more]
A0A6J1E1Z52.7e-6584.42uncharacterized protein LOC111025692 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1H5029.6e-6380.89caltractin-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111460448 PE=4 SV... [more]
A0A6J1KWC62.1e-6280.25caltractin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499295 PE=4 SV=1[more]
A0A6J1DY882.8e-5479.45uncharacterized protein LOC111025692 isoform X2 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
AT1G73440.16.0e-2543.59calmodulin-related [more]
AT4G00140.14.8e-0648.84Calcium-binding EF-hand family protein [more]
AT3G50360.14.5e-0431.82centrin2 [more]
AT4G37010.14.5e-0432.31centrin 2 [more]
AT4G37010.24.5e-0432.31centrin 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 139..167
e-value: 5.8E-4
score: 29.2
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 135..161
e-value: 1.0E-5
score: 25.2
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 135..170
score: 11.640179
NoneNo IPR availableGENE3D6.10.140.100coord: 8..68
e-value: 3.5E-5
score: 25.7
NoneNo IPR availableGENE3D1.10.238.10coord: 86..174
e-value: 4.3E-11
score: 44.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..19
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..45
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 148..160
IPR003903Ubiquitin interacting motifPROSITEPS50330UIMcoord: 42..61
score: 9.2238
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 93..164

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg024128.1Spg024128.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding