Spg024058 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg024058
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionOxoglutarate/iron-dependent dioxygenase
Locationscaffold13: 993883 .. 998695 (+)
RNA-Seq ExpressionSpg024058
SyntenySpg024058
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTAGGATCAAAAGGGTTAATGAAACTTGGATTAATCAAAGCCCAAGCTGGATATGTAATTGGGCCTTGTATGACCATTTTTCTGATGCCCATGTGGAGTAATAAAATGTCTTTATTAAAAAAAAAAAAAAAAATAGGTTGGATGGACTCCAATTTATCAATGTAAGATTTCATGCATTAATTCAATTTTTATTCTTCCCTAAATGTCCTTATTTAATTTGGTAACTCTTATTTTATTAATTTATTTTAGTATTATATTTTTTTTCTGTCAACCGATAAGGGCAAATACGTAAATGGACTTCTTAAACTCATTTAATATCCGATTGTGAGATGACAAAAATGTAAAATTATAACAAAATAGACACTTCTGAAATATGAAACACTTGTTCACAAAATAAAATAAAATAAATCAGTTACAAATGTGAAATCTTAGAACTGACGTTAATGGAATTTTACCTAGTTATCTTAAACGTCATCAAGGCCCAACGAAACGACAGTAAAAGTAAGAAGTAAAACTAGACGAACTTTGTTAAAGAAGGAGGAGAAAAAAAAAAAAAAAAAAAAAAAAAGGCAAAACCTCTCTCAATCCCCTGCGATCTCTCTCCATCACTTTCCCGACTTCTCTCGATCGCTCCTCCGCTTCAAGGTTTGTTCGTGATTGAATGCGTTTTTTTCTTTTCAAGATTTTGCTTGGTTAACAACTTACAACTGTTGCAATCACGATTGGAATTTTTTTTCTCTGTTTTATTCTGCTCGATCTTTCCCTCTCACAGCCCCAGAATGTTGTTTGAATGTGAAATATTGAAGTGTAATTAGCAATTAGCAATTCTTTGTTTATAATCTCATATCTTAGCAATCCTTTCAATGATCAATGCCAGCGGCTTGCTGCAATATTCGTTTGCAAGTGTCTTTTGAATTGTTTAATTGATTGAAGTGTATGTTTCTGGTTTTGTGGCGGCTGCGGAGTGTTGAATTTGGTTATTTTTCACTCTACTTTGCTGCTCGATTAGTGTTTGGTTGATTATCTGCTTGTTGATGGTTTGAAGGTTTTCTTTTTCTTATGGAAAAAGAAATAGCCTCAAATGTATAGGCTGTGTTTAGTTCGGGATTTATTTGTTCTCTCTGCGATGATTATCTACTTTATGTTGTGTCTATGATGATTTGTTTAGCTGACTGTTAATTTCTATTTTCTTGATTAGTTTATTGATCTCTTTTCTTTTCATTTGAAGTTCATATTTAAACCGTCTGTCTCTGACTGTCATTGATGCTAAGAATATAACTGTTCTCATGTCTGCTACAGATTTGTCATTGAAATTGATCAAGCGCATTGCAAACGATTCAGCCTCCTTCGGCATTCAATTTCCCTCGATCAATCTCTTCTATTTCCAGTTCCCTTTCAGGATCATGAATCACGGTGGCCCTAGATATGCTGGAAGAGGCCACCCGAATAACAGAGGACGATCTCCTCGCTCTGCTGATAATTCCACGTATCGTCCCCGTCATGTAACTTTCTTTAAGCTCCACGATGTTTACAAACATTAGGCATATTGTCTCTAGCCTTTCTCTCTGCTTTCTTCGTTTATTTCTTTATTCTTGCCTTGCCTATAAAACGATCAAGTTGACTATTGAATCTGTCCAATGTAGTTGTGTTTTCTTTTCTTTTTCCTTCAAGATGAAGAATTGATGACATTCCTTATGCAAATCGATATTTAGTTGAAATTGTAAAGAAAAGTTGGGATTTAAGTAAATTACCCCTTTGTGTTTTTTGTCTCAGTGGTGGATTACGAGAATGACTTGAGGAAGATTTATGGTGAAATTGTTTGTGGAATTTTGAAATTTGTAGGTGGTTGATTTTGTTTGCAAATTTATGAAAATGCCGTGTATGTTAGGGAGGAATTGTATCCATTAGTATTTTTCAAAAAAAATTAATTGTATCCATTAGTAAGGGGATGGAATGAAGGTGCTGGAGTTTAAATTTTTTACTCTCATGCTTACTTCTATTCTTTGCACAATCTATCAAAAAAAGCAGGCTGGAGGTGAAGGGCCTTCTGTTCCAGGTTCTTCTAATCCTAGTGGTGGAGCTTTCCGAGAGAGAAGTTTTCACCAGATGCCATCAAGGAATTTTAGGAAGCAATTTGTGCAAAAACAGGCCTCAAGTTCTGAACAGCAGCAGTGGCGGCCTATAAATAGTGGGAAAGATGCATCTCCTGGTGCTGTTGATCTTCAGCAGGAACACAAATTGGCCGATGATATGAGCAATACCTCTAAACAATCATTGGGATCTGTTGCATCAGATTCTGATGGCAATCTTATGAGCAATACCTCTGAACAACTATTGGGATCTATTGCATCAAATTCTGATTGCAATGAACTCTCACCCTCTTCTGCTCAAAATGTCTCTAAGAGTTTGCATTCTGCTGTAGAAAGAATTCAGATTCGAGAACCTATAGCAGAAGGGGGAAGCTGCAGTACTTCATTGCCTTATGATAATTGTAACAGATCAGATGCTGTTGAACAGGAACTCATTGTTCAAGTTCCCTTGGAATCCTGTGCTAAAGATGAGGGTTCAACCAGAAAATTAAGGGAAAATGATATTTCTTACTGTAAAGACTCAGAGGACAAAAAGCCTTCAGTGAACCTTAATCCGTTTGATATATGCCCTCCAAAGTCTGGAGGTATTAAGCTGAATCCTTCTTTATTAGCTAAGAACAGAGAAAAAAGGAATGAGATGAAGCGTGCAATGGAGGGAAACAGTGGAGTTGTGTTGAGACCGGGAATGGTTCATCTGAAGAGTGGCATTTCCCTCAGGGATCAGGTATACGTTGGTTCTCTCTCTCATCTGCATAAATGATGTATATCAAAATCTTGTTCTGTAGAAATAGCCTCAAACATAACTGAATCAGTTCTTGAGTTAAAGATCTTTCCATATATACTAAGGTGGGAAAGCTCACTTTTCAAGGATGGGGAAAAAATGATACTACACTTTCCCACAAAAGAAGTCCAAACACTAAGCACAAATGGACTCCAATCCAAAAGATTAGATCACGCTGATAATTACAAAAAATCTGAGGAAAGATGCTTTATTGTCAAGCAGAAAATGGATCTTATACGGACGGTTTGTAGTTTCCACTATGGTCTGTGTTTCTCTTTCATACGGGGTTTACCATATAGGTAGTTTGATTAGAGCCTGCCACACTAATATTTCTGGCTTTGTTGGGAGTGTTTTCAAAATTCTGAATCTTTAAGACCTGCTTTCCTTACTGGTACTTTTCCATCTTTATTGTGCTCGCAGGTGAAGATAGTAAAAAGATGTCGGGATCTTGGTATAGGAGCTGGAGGTTTTTACCAACCTGGTTACCGTGAAGGAGGAAAACTACACTTGAAGATGATGTGCCTTGGTAAAAATTGGGATCCTGACAGTAGTTCATATGGGGATGTCCGCCCATTTGATGGTACAAAACCACCAGGCATTCCAAATGAATTTTACCAATTGGTTGAAAAGGCAATCAAAGATTCTTATGCCATTATGGAGAAAGATTCAGCAACCAAAAATCCTGAACGTGTACTTCCATGGATGAAACCAAACATCTGTATTGTAAACTTTTACTCACAAAATGGGCGATTGGGTCTTCATCAGGTGTGCAATATGACTCCTGTGCTCTTCATTTTTTGAACTGGCCCAGCATTTGCTTACTTTTTCCATGACAATCTTTGTTTAATTGCCTCCCAAAAAGCATGTAACTGACTATTTTCCTCCCATTAGGATCGAGATGAAAGTCAAGAAAGTCTTGAAAAAGGGCTGCCTGTTGTCTCCTTCTCCATTGGTGACTCTGCAGAATTTCTATTTGGTGATCGGAGTGACACTGACCAAGCAGAGAAAGTTATTTTGGAATCCGGAGATATTTTGATATTTGGTGGAAAATCAAGACATGTTTTCCATGGGGTGACTGCAATTCATCCAAACACTGCTCCGAAGGCACTTTTAGACGAAACAAATATTTGTCCAGGTCGCTTGAATCTTACTTTCCGTCAGTACTGAAAGTATATTGGTCTCAGTTAGTTCTTAGCCGCTTTTTTGCTCAGCGAAAGAGATGTCCATGAATCAACGCCCTTATAACACAGTTCTAGGCTTCCTCTTTAGTGTATCGGGGAGTTAGTTTTGTTTTATTATGTAATGAATGTCTGTATATATTGGCCTTGCAACACAATACAAAAGTTTCCCTTCTGGAATCCCTATCTAATCTTGATACATGTTGAGAGTATGTAATAGACTCTCTAGAGATATTCTCCTCGTTTTAGTCTATTCTCCTTTTGGGATGAAAAGGGAGCTTGACCATCTTTCATGGGATTGCCCTTTTACTTTCTCCCTCTGCAGTTGGTTTTCAGGACTTCTGGTTTGTTTTGGCTCGCAACAGGGGGCCGCTTTATGATTGATGATGTGCTTCTAAACTCTCCTTTTTGCAATAAAGAGTTTTGTGGGAAACTCTTTTTTTTTTTTTTTTCCTCTCTCTTACCATTTTGTGGGGTGTTTAGTTCGGGAGGGACAATAGGATTTATAGAGGGTTCGAGAGATTGAAGAGGAGCGTTGGTTGTTGGTGTTGGTTTTAACTCCTTTTTGAGCCACCATAATCTGGTTTTTTGTAATTGTCATATCTTTTAGATACATAATTACCATCTTGGTCTCATTCTTTTGGATTGGAGTCCGATCTTGTAGCTAGTGTTTGGAGCTCCTTTTTGTGGATTTGTTTTTTGTATGTCCTTGTATATTCTTTCATTGAATGAAAGTTCGATTTCTTATTATTGAAAACAAA

mRNA sequence

ATGCTAGGATCAAAAGGGTTAATGAAACTTGGATTAATCAAAGCCCAAGCTGGATATGTAATTGGGCCTTATTTGTCATTGAAATTGATCAAGCGCATTGCAAACGATTCAGCCTCCTTCGGCATTCAATTTCCCTCGATCAATCTCTTCTATTTCCAGTTCCCTTTCAGGATCATGAATCACGGTGGCCCTAGATATGCTGGAAGAGGCCACCCGAATAACAGAGGACGATCTCCTCGCTCTGCTGATAATTCCACGTATCGTCCCCGTCATGCTGGAGGTGAAGGGCCTTCTGTTCCAGGTTCTTCTAATCCTAGTGGTGGAGCTTTCCGAGAGAGAAGTTTTCACCAGATGCCATCAAGGAATTTTAGGAAGCAATTTGTGCAAAAACAGGCCTCAAGTTCTGAACAGCAGCAGTGGCGGCCTATAAATAGTGGGAAAGATGCATCTCCTGGTGCTGTTGATCTTCAGCAGGAACACAAATTGGCCGATGATATGAGCAATACCTCTAAACAATCATTGGGATCTGTTGCATCAGATTCTGATGGCAATCTTATGAGCAATACCTCTGAACAACTATTGGGATCTATTGCATCAAATTCTGATTGCAATGAACTCTCACCCTCTTCTGCTCAAAATGTCTCTAAGAGTTTGCATTCTGCTGTAGAAAGAATTCAGATTCGAGAACCTATAGCAGAAGGGGGAAGCTGCAGTACTTCATTGCCTTATGATAATTGTAACAGATCAGATGCTGTTGAACAGGAACTCATTGTTCAAGTTCCCTTGGAATCCTGTGCTAAAGATGAGGGTTCAACCAGAAAATTAAGGGAAAATGATATTTCTTACTGTAAAGACTCAGAGGACAAAAAGCCTTCAGTGAACCTTAATCCGTTTGATATATGCCCTCCAAAGTCTGGAGGTATTAAGCTGAATCCTTCTTTATTAGCTAAGAACAGAGAAAAAAGGAATGAGATGAAGCGTGCAATGGAGGGAAACAGTGGAGTTGTGTTGAGACCGGGAATGGTTCATCTGAAGAGTGGCATTTCCCTCAGGGATCAGGTGAAGATAGTAAAAAGATGTCGGGATCTTGGTATAGGAGCTGGAGGTTTTTACCAACCTGGTTACCGTGAAGGAGGAAAACTACACTTGAAGATGATGTGCCTTGGTAAAAATTGGGATCCTGACAGTAGTTCATATGGGGATGTCCGCCCATTTGATGGTACAAAACCACCAGGCATTCCAAATGAATTTTACCAATTGGTTGAAAAGGCAATCAAAGATTCTTATGCCATTATGGAGAAAGATTCAGCAACCAAAAATCCTGAACGTGTACTTCCATGGATGAAACCAAACATCTGTATTGTAAACTTTTACTCACAAAATGGGCGATTGGGTCTTCATCAGGATCGAGATGAAAGTCAAGAAAGTCTTGAAAAAGGGCTGCCTGTTGTCTCCTTCTCCATTGGTGACTCTGCAGAATTTCTATTTGGTGATCGGAGTGACACTGACCAAGCAGAGAAAGTTATTTTGGAATCCGGAGATATTTTGATATTTGGTGGAAAATCAAGACATGTTTTCCATGGGGTGACTGCAATTCATCCAAACACTGCTCCGAAGGCACTTTTAGACGAAACAAATATTTGTCCAGGTCGCTTGAATCTTACTTTCCGTCAGTACTGA

Coding sequence (CDS)

ATGCTAGGATCAAAAGGGTTAATGAAACTTGGATTAATCAAAGCCCAAGCTGGATATGTAATTGGGCCTTATTTGTCATTGAAATTGATCAAGCGCATTGCAAACGATTCAGCCTCCTTCGGCATTCAATTTCCCTCGATCAATCTCTTCTATTTCCAGTTCCCTTTCAGGATCATGAATCACGGTGGCCCTAGATATGCTGGAAGAGGCCACCCGAATAACAGAGGACGATCTCCTCGCTCTGCTGATAATTCCACGTATCGTCCCCGTCATGCTGGAGGTGAAGGGCCTTCTGTTCCAGGTTCTTCTAATCCTAGTGGTGGAGCTTTCCGAGAGAGAAGTTTTCACCAGATGCCATCAAGGAATTTTAGGAAGCAATTTGTGCAAAAACAGGCCTCAAGTTCTGAACAGCAGCAGTGGCGGCCTATAAATAGTGGGAAAGATGCATCTCCTGGTGCTGTTGATCTTCAGCAGGAACACAAATTGGCCGATGATATGAGCAATACCTCTAAACAATCATTGGGATCTGTTGCATCAGATTCTGATGGCAATCTTATGAGCAATACCTCTGAACAACTATTGGGATCTATTGCATCAAATTCTGATTGCAATGAACTCTCACCCTCTTCTGCTCAAAATGTCTCTAAGAGTTTGCATTCTGCTGTAGAAAGAATTCAGATTCGAGAACCTATAGCAGAAGGGGGAAGCTGCAGTACTTCATTGCCTTATGATAATTGTAACAGATCAGATGCTGTTGAACAGGAACTCATTGTTCAAGTTCCCTTGGAATCCTGTGCTAAAGATGAGGGTTCAACCAGAAAATTAAGGGAAAATGATATTTCTTACTGTAAAGACTCAGAGGACAAAAAGCCTTCAGTGAACCTTAATCCGTTTGATATATGCCCTCCAAAGTCTGGAGGTATTAAGCTGAATCCTTCTTTATTAGCTAAGAACAGAGAAAAAAGGAATGAGATGAAGCGTGCAATGGAGGGAAACAGTGGAGTTGTGTTGAGACCGGGAATGGTTCATCTGAAGAGTGGCATTTCCCTCAGGGATCAGGTGAAGATAGTAAAAAGATGTCGGGATCTTGGTATAGGAGCTGGAGGTTTTTACCAACCTGGTTACCGTGAAGGAGGAAAACTACACTTGAAGATGATGTGCCTTGGTAAAAATTGGGATCCTGACAGTAGTTCATATGGGGATGTCCGCCCATTTGATGGTACAAAACCACCAGGCATTCCAAATGAATTTTACCAATTGGTTGAAAAGGCAATCAAAGATTCTTATGCCATTATGGAGAAAGATTCAGCAACCAAAAATCCTGAACGTGTACTTCCATGGATGAAACCAAACATCTGTATTGTAAACTTTTACTCACAAAATGGGCGATTGGGTCTTCATCAGGATCGAGATGAAAGTCAAGAAAGTCTTGAAAAAGGGCTGCCTGTTGTCTCCTTCTCCATTGGTGACTCTGCAGAATTTCTATTTGGTGATCGGAGTGACACTGACCAAGCAGAGAAAGTTATTTTGGAATCCGGAGATATTTTGATATTTGGTGGAAAATCAAGACATGTTTTCCATGGGGTGACTGCAATTCATCCAAACACTGCTCCGAAGGCACTTTTAGACGAAACAAATATTTGTCCAGGTCGCTTGAATCTTACTTTCCGTCAGTACTGA

Protein sequence

MLGSKGLMKLGLIKAQAGYVIGPYLSLKLIKRIANDSASFGIQFPSINLFYFQFPFRIMNHGGPRYAGRGHPNNRGRSPRSADNSTYRPRHAGGEGPSVPGSSNPSGGAFRERSFHQMPSRNFRKQFVQKQASSSEQQQWRPINSGKDASPGAVDLQQEHKLADDMSNTSKQSLGSVASDSDGNLMSNTSEQLLGSIASNSDCNELSPSSAQNVSKSLHSAVERIQIREPIAEGGSCSTSLPYDNCNRSDAVEQELIVQVPLESCAKDEGSTRKLRENDISYCKDSEDKKPSVNLNPFDICPPKSGGIKLNPSLLAKNREKRNEMKRAMEGNSGVVLRPGMVHLKSGISLRDQVKIVKRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSSYGDVRPFDGTKPPGIPNEFYQLVEKAIKDSYAIMEKDSATKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESLEKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGDILIFGGKSRHVFHGVTAIHPNTAPKALLDETNICPGRLNLTFRQY
Homology
BLAST of Spg024058 vs. NCBI nr
Match: XP_038875730.1 (uncharacterized protein LOC120068103 isoform X1 [Benincasa hispida])

HSP 1 Score: 820.1 bits (2117), Expect = 1.2e-233
Identity = 415/502 (82.67%), Postives = 443/502 (88.25%), Query Frame = 0

Query: 59  MNHGGPRYAGRGHPNNRGRSPRSADNSTYRPRHAGGEGPSVPGSSNPSGGAFRERSFHQM 118
           MN GG RYAGRGHPNNRGRSPRSADN TYRPRHAGGEGPSV GSSNP  G FR+RS   M
Sbjct: 1   MNDGGRRYAGRGHPNNRGRSPRSADNFTYRPRHAGGEGPSVAGSSNPGNGTFRDRSSQHM 60

Query: 119 PSRNFRKQFVQKQASSSEQQQWRPINSGKDASPGAVDLQQEHKLADDMSNTSKQSLGSVA 178
            SRN  K F QKQASSSEQ QWRP+NSGKDAS  A+DLQ EH  ADD+SNTSKQ LGS+A
Sbjct: 61  SSRNVGKPFGQKQASSSEQWQWRPLNSGKDASSAAIDLQPEHNSADDLSNTSKQLLGSIA 120

Query: 179 SDSDGNLMSNTSEQLLGSIASNSDCNELSPSSAQNVSKSLHSAVERIQIREPIAEGGSCS 238
           S++D N MS +S+QLLGSIASNS+C E SPSSAQNVSKSLHSAVERIQIRE  A G SCS
Sbjct: 121 SNTDSNHMSKSSKQLLGSIASNSNCKEFSPSSAQNVSKSLHSAVERIQIRESTAVGESCS 180

Query: 239 TSLPYDNCNRSDAVEQELIVQVPLESCAKDEGSTRKLRE-NDISYCKDSEDKKPSVNLNP 298
            S P+DNCNRSDAV Q+L VQV LESC KDE ST+KLRE N++S   DS+DKKPSVNL+P
Sbjct: 181 DSFPHDNCNRSDAVGQDLKVQVLLESCVKDESSTKKLRESNNVSGSTDSKDKKPSVNLDP 240

Query: 299 FDICPPKSGGIKLNPSLLAKNREKRNEMKRAMEGNSGVVLRPGMVHLKSGISLRDQVKIV 358
           FDIC PK+G + LNPSL AKNREKRNEMKRAMEGNSG+VLRPGMVHLKSGISLRDQV IV
Sbjct: 241 FDICLPKTGVVTLNPSLFAKNREKRNEMKRAMEGNSGIVLRPGMVHLKSGISLRDQVMIV 300

Query: 359 KRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSSYGDVRPFDGTKPPGIPNEF 418
           KRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSS+YGDVRPFD TKPP +P+EF
Sbjct: 301 KRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDDTKPPSLPDEF 360

Query: 419 YQLVEKAIKDSYAIMEKDSATKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 478
           YQLVEKAIK SYAIM KDS  KNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDES+ESL
Sbjct: 361 YQLVEKAIKVSYAIMGKDSTMKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESRESL 420

Query: 479 EKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGDILIFGGKSRHVFHGVTAIHPNTA 538
           +KGLPVVSFSIGDSAEFLFGD+SD DQAEKV LESGDILIFGGKSRHVFHGVT IHPNTA
Sbjct: 421 DKGLPVVSFSIGDSAEFLFGDQSDADQAEKVTLESGDILIFGGKSRHVFHGVTVIHPNTA 480

Query: 539 PKALLDETNICPGRLNLTFRQY 560
           PK LL+ TN+ PGRLNLTFRQY
Sbjct: 481 PKELLEATNLRPGRLNLTFRQY 502

BLAST of Spg024058 vs. NCBI nr
Match: KAA0058530.1 (Oxoglutarate/iron-dependent dioxygenase [Cucumis melo var. makuwa] >TYK07216.1 Oxoglutarate/iron-dependent dioxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 804.7 bits (2077), Expect = 5.1e-229
Identity = 409/502 (81.47%), Postives = 437/502 (87.05%), Query Frame = 0

Query: 59  MNHGGPRYAGRGHPNNRGRSPRSADNSTYRPRHAGGEGPSVPGSSNPSGGAFRERSFHQM 118
           MN GGPRYAGRGHPNNRGRSPRSADN TYRPRHAGGEG SV GSSNP  GAFR RS HQM
Sbjct: 1   MNDGGPRYAGRGHPNNRGRSPRSADNFTYRPRHAGGEGSSVAGSSNPGSGAFRGRSSHQM 60

Query: 119 PSRNFRKQFVQKQASSSEQQQWRPINSGKDASPGAVDLQQEHKLADDMSNTSKQSLGSVA 178
            SRNFRK   QKQASSSEQ QWRP+NS KDASPGAVDL  +H   DDMSNT+KQ LGS+A
Sbjct: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSRKDASPGAVDLHLQHNSTDDMSNTNKQLLGSIA 120

Query: 179 SDSDGNLMSNTSEQLLGSIASNSDCNELSPSSAQNVSKSLHSAVERIQIREPIAEGGSCS 238
           S+SD N +SNTSEQ LG IASNSDC EL  SSAQNVSKSLHSAVERIQI+ P A  G+CS
Sbjct: 121 SNSDSNQISNTSEQRLGPIASNSDCIELLSSSAQNVSKSLHSAVERIQIQGPTAVCGNCS 180

Query: 239 TSLPYDNCNRSDAVEQELIVQVPLESCAKDEGSTRKLRE-NDISYCKDSEDKKPSVNLNP 298
            S PYDN N SD V QEL VQ  LESCAKD+ ST KL E N++    DS+DKKPSV+L+ 
Sbjct: 181 DSFPYDNRNGSDVVGQELKVQPTLESCAKDKSSTIKLGESNNVFNSMDSKDKKPSVDLDT 240

Query: 299 FDICPPKSGGIKLNPSLLAKNREKRNEMKRAMEGNSGVVLRPGMVHLKSGISLRDQVKIV 358
           FDICPPK+GG+ LNPSLLA NREKRNEMKRAM+GN+G+VLRPGMVHLK  ISLRDQ KIV
Sbjct: 241 FDICPPKTGGVTLNPSLLAMNREKRNEMKRAMDGNNGIVLRPGMVHLKGSISLRDQAKIV 300

Query: 359 KRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSSYGDVRPFDGTKPPGIPNEF 418
           K+CRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSS+YGDVRPFD TKPP +P+EF
Sbjct: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDDTKPPNLPDEF 360

Query: 419 YQLVEKAIKDSYAIMEKDSATKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 478
           YQLVEKAIKDSYAI+ KDS  KNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL
Sbjct: 361 YQLVEKAIKDSYAIIAKDSTIKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 420

Query: 479 EKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGDILIFGGKSRHVFHGVTAIHPNTA 538
           +KGLPV+SFSIGDSAEFLFGD SD DQAEKV LESGDILIFGGKSRHVFHGVTAIH NTA
Sbjct: 421 DKGLPVISFSIGDSAEFLFGDWSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA 480

Query: 539 PKALLDETNICPGRLNLTFRQY 560
           PKALL+ TN+ PGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 502

BLAST of Spg024058 vs. NCBI nr
Match: XP_016903133.1 (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103501985 [Cucumis melo])

HSP 1 Score: 798.9 bits (2062), Expect = 2.8e-227
Identity = 408/502 (81.27%), Postives = 436/502 (86.85%), Query Frame = 0

Query: 59  MNHGGPRYAGRGHPNNRGRSPRSADNSTYRPRHAGGEGPSVPGSSNPSGGAFRERSFHQM 118
           MN GGPRYAGRGHPNNRGRSPRSADN TYRPRHAGGEG SV GSSNP  GAFR RS HQM
Sbjct: 1   MNDGGPRYAGRGHPNNRGRSPRSADNFTYRPRHAGGEGSSVAGSSNPGSGAFRGRSSHQM 60

Query: 119 PSRNFRKQFVQKQASSSEQQQWRPINSGKDASPGAVDLQQEHKLADDMSNTSKQSLGSVA 178
            SRNFRK   QKQASSSEQ QWRP+NS KDASPGAVDL  +H   DDMSNT+KQ LGS+A
Sbjct: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSRKDASPGAVDLHLQHNSTDDMSNTNKQLLGSIA 120

Query: 179 SDSDGNLMSNTSEQLLGSIASNSDCNELSPSSAQNVSKSLHSAVERIQIREPIAEGGSCS 238
           S+SD N +SNTSEQ LG IASNSDC EL  SSAQNVSKSLHSAVERIQI+ P A  G+CS
Sbjct: 121 SNSDSNQISNTSEQRLGPIASNSDCIELLSSSAQNVSKSLHSAVERIQIQGPTAVCGNCS 180

Query: 239 TSLPYDNCNRSDAVEQELIVQVPLESCAKDEGSTRKLRE-NDISYCKDSEDKKPSVNLNP 298
            S PYDN N SD V QEL VQ  LESCAKD+ ST KL E N++    DS+DKKPSV+L+ 
Sbjct: 181 DSFPYDNRNGSDVVGQELKVQPTLESCAKDKSSTIKLGESNNVFNSMDSKDKKPSVDLDT 240

Query: 299 FDICPPKSGGIKLNPSLLAKNREKRNEMKRAMEGNSGVVLRPGMVHLKSGISLRDQVKIV 358
           FDICPPK+GG+ LNPSLLA NREK NEMKRAM+GN+G+VLRPGMVHLK  ISLRDQ KIV
Sbjct: 241 FDICPPKTGGVTLNPSLLAMNREK-NEMKRAMDGNNGIVLRPGMVHLKGSISLRDQAKIV 300

Query: 359 KRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSSYGDVRPFDGTKPPGIPNEF 418
           K+CRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSS+YGDVRPFD TKPP +P+EF
Sbjct: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDDTKPPNLPDEF 360

Query: 419 YQLVEKAIKDSYAIMEKDSATKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 478
           YQLVEKAIKDSYAI+ KDS  KNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL
Sbjct: 361 YQLVEKAIKDSYAIIAKDSTIKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 420

Query: 479 EKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGDILIFGGKSRHVFHGVTAIHPNTA 538
           +KGLPV+SFSIGDSAEFLFGDRSD DQAEKV LESGDILIFGGKSRHVFHGVTAIH  TA
Sbjct: 421 DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSKTA 480

Query: 539 PKALLDETNICPGRLNLTFRQY 560
           PKALL+ TN+ PGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 501

BLAST of Spg024058 vs. NCBI nr
Match: KAG6581809.1 (hypothetical protein SDJN03_21811, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 783.5 bits (2022), Expect = 1.2e-222
Identity = 405/502 (80.68%), Postives = 432/502 (86.06%), Query Frame = 0

Query: 59  MNHGGPRYAGRGHPNNRGRSPRSADNSTYRPRHAGGEGPSVPGSSNPSGGAFRERSFHQM 118
           MN GG RY GRGHPNNRGR PRSADN TYRPRHAGGEGPS  GSSNP  GAF++RS H  
Sbjct: 1   MNDGGHRYGGRGHPNNRGRFPRSADNFTYRPRHAGGEGPSAAGSSNPRNGAFKDRSSH-- 60

Query: 119 PSRNFRKQFVQKQASSSEQQQWRPINSGKDASPGAVDLQQEHKLADDMSNTSKQSLGSVA 178
           PSRN R  F QKQA  SEQ QWRP+NSGKDAS GAVDLQQ+H  ADD++   KQ +GS++
Sbjct: 61  PSRNSRNTFGQKQALGSEQWQWRPLNSGKDASAGAVDLQQDHNTADDIA--PKQLIGSIS 120

Query: 179 SDSDGNLMSNTSEQLLGSIASNSDCNELSPSSAQNVSKSLHSAVERIQIREPIAEGGSCS 238
           S+SDGN MSNTSEQLLGSIAS SD NE SPSSAQNVSKSLHSAVERIQIREP AEG  C+
Sbjct: 121 SNSDGNHMSNTSEQLLGSIASKSDGNEHSPSSAQNVSKSLHSAVERIQIREPTAEGERCN 180

Query: 239 TSLPYDNCNRSDAVEQELIVQVPLESCAKDEGSTRKLRE-NDISYCKDSEDKKPSVNLNP 298
            S PYD+C RSDA  QEL+VQ        D+ +T KLRE N++S CKDS+DKKP  NL  
Sbjct: 181 DSFPYDSCKRSDAGGQELMVQ--------DQTATIKLRESNNVSDCKDSKDKKPLGNLEI 240

Query: 299 FDICPPKSGGIKLNPSLLAKNREKRNEMKRAMEGNSGVVLRPGMVHLKSGISLRDQVKIV 358
           FDICPPKSG + LNPSLL+KNREKRNEMKRAMEGN+G VLRPGMVHLKSGISL DQVKIV
Sbjct: 241 FDICPPKSGVVTLNPSLLSKNREKRNEMKRAMEGNNGNVLRPGMVHLKSGISLSDQVKIV 300

Query: 359 KRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSSYGDVRPFDGTKPPGIPNEF 418
           K+CRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSS YGDVRPFD T PP +P EF
Sbjct: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSKYGDVRPFDNTTPPNMPVEF 360

Query: 419 YQLVEKAIKDSYAIMEKDSATKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 478
           Y+LVEKAIKDSYA+M KDS TKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL
Sbjct: 361 YKLVEKAIKDSYAVMGKDSNTKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 420

Query: 479 EKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGDILIFGGKSRHVFHGVTAIHPNTA 538
           EKGLPVVSFSIGDSAEFLFGDRSD DQAEKV LESGDILIFGGKSRHVFHGVTAIH NTA
Sbjct: 421 EKGLPVVSFSIGDSAEFLFGDRSDIDQAEKVTLESGDILIFGGKSRHVFHGVTAIHQNTA 480

Query: 539 PKALLDETNICPGRLNLTFRQY 560
           PKALL+ TN+ PGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 490

BLAST of Spg024058 vs. NCBI nr
Match: KAG7018258.1 (alkB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 783.1 bits (2021), Expect = 1.6e-222
Identity = 405/502 (80.68%), Postives = 431/502 (85.86%), Query Frame = 0

Query: 59  MNHGGPRYAGRGHPNNRGRSPRSADNSTYRPRHAGGEGPSVPGSSNPSGGAFRERSFHQM 118
           MN GG RY GRGHPNNRGR PRSADN TYRPRHAGGEGPS  GSSNP  GAF++RS H  
Sbjct: 1   MNDGGHRYGGRGHPNNRGRFPRSADNFTYRPRHAGGEGPSAAGSSNPRNGAFKDRSSH-- 60

Query: 119 PSRNFRKQFVQKQASSSEQQQWRPINSGKDASPGAVDLQQEHKLADDMSNTSKQSLGSVA 178
           PSRN R  F QKQA  SEQ QWRP+NSGKDAS GAVDLQQ+H  ADD++   KQ +GS++
Sbjct: 61  PSRNSRNTFGQKQALGSEQWQWRPLNSGKDASAGAVDLQQDHNTADDIA--PKQLIGSIS 120

Query: 179 SDSDGNLMSNTSEQLLGSIASNSDCNELSPSSAQNVSKSLHSAVERIQIREPIAEGGSCS 238
           S+SDGN MSNTSEQLLGSIAS SD NE SPSSAQNVSKSLHSAVERIQIREP AEG  C 
Sbjct: 121 SNSDGNHMSNTSEQLLGSIASKSDGNEHSPSSAQNVSKSLHSAVERIQIREPTAEGERCD 180

Query: 239 TSLPYDNCNRSDAVEQELIVQVPLESCAKDEGSTRKLRE-NDISYCKDSEDKKPSVNLNP 298
            S PYD+C RSDA  QEL+VQ        D+ +T KLRE N++S CKDS+DKKP  NL  
Sbjct: 181 DSFPYDSCKRSDAGGQELMVQ--------DQTATIKLRESNNVSDCKDSKDKKPLGNLEI 240

Query: 299 FDICPPKSGGIKLNPSLLAKNREKRNEMKRAMEGNSGVVLRPGMVHLKSGISLRDQVKIV 358
           FDICPPKSG + LNPSLL+KNREKRNEMKRAMEGN+G VLRPGMVHLKSGISL DQVKIV
Sbjct: 241 FDICPPKSGVVTLNPSLLSKNREKRNEMKRAMEGNNGNVLRPGMVHLKSGISLSDQVKIV 300

Query: 359 KRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSSYGDVRPFDGTKPPGIPNEF 418
           K+CRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSS YGDVRPFD T PP +P EF
Sbjct: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSKYGDVRPFDNTTPPNMPVEF 360

Query: 419 YQLVEKAIKDSYAIMEKDSATKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 478
           Y+LVEKAIKDSYA+M KDS TKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL
Sbjct: 361 YKLVEKAIKDSYAVMGKDSNTKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 420

Query: 479 EKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGDILIFGGKSRHVFHGVTAIHPNTA 538
           EKGLPVVSFSIGDSAEFLFGDRSD DQAEKV LESGDILIFGGKSRHVFHGVTAIH NTA
Sbjct: 421 EKGLPVVSFSIGDSAEFLFGDRSDIDQAEKVTLESGDILIFGGKSRHVFHGVTAIHQNTA 480

Query: 539 PKALLDETNICPGRLNLTFRQY 560
           PKALL+ TN+ PGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 490

BLAST of Spg024058 vs. ExPASy Swiss-Prot
Match: P05050 (Alpha-ketoglutarate-dependent dioxygenase AlkB OS=Escherichia coli (strain K12) OX=83333 GN=alkB PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 7.9e-15
Identity = 48/160 (30.00%), Postives = 78/160 (48.75%), Query Frame = 0

Query: 399 YGDVRPFDGTKPPGIPNEFYQLVEKAIKDSYAIMEKDSATKNPERVLPWMKPNICIVNFY 458
           Y  + P      P +P  F+ L ++A           +A   P+      +P+ C++N Y
Sbjct: 78  YSPIDPQTNKPWPAMPQSFHNLCQRAA----------TAAGYPD-----FQPDACLINRY 137

Query: 459 SQNGRLGLHQDRDESQESLEKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGDILIF 518
           +   +L LHQD+DE     +   P+VS S+G  A F FG     D  ++++LE GD++++
Sbjct: 138 APGAKLSLHQDKDEP----DLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVW 197

Query: 519 GGKSRHVFHGVTAIHPNTAPKALLDETNICPGRLNLTFRQ 559
           GG+SR  +HG+  +     P  +         R NLTFRQ
Sbjct: 198 GGESRLFYHGIQPLKAGFHPLTI-------DCRYNLTFRQ 211

BLAST of Spg024058 vs. ExPASy Swiss-Prot
Match: P37462 (Alpha-ketoglutarate-dependent dioxygenase AlkB OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=alkB PE=3 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 8.8e-14
Identity = 42/114 (36.84%), Postives = 63/114 (55.26%), Query Frame = 0

Query: 449 KPNICIVNFYSQNGRLGLHQDRDESQESLEKGLPVVSFSIGDSAEFLFGDRSDTDQAEKV 508
           +P+ C++N Y+   +L LHQD+DE     +   P+VS S+G  A F FG    +D  +++
Sbjct: 113 QPDACLINRYAPGAKLSLHQDKDEP----DLRAPIVSVSLGVPAVFQFGGLRRSDPIQRI 172

Query: 509 ILESGDILIFGGKSRHVFHGV----TAIHPNTAPKALLDETNICPGRLNLTFRQ 559
           +LE GDI+++GG+SR  +HG+       HP T              R NLTFRQ
Sbjct: 173 LLEHGDIVVWGGESRLFYHGIQPLKAGFHPMTG-----------EFRYNLTFRQ 211

BLAST of Spg024058 vs. ExPASy Swiss-Prot
Match: P0CAT7 (Alpha-ketoglutarate-dependent dioxygenase AlkB homolog OS=Caulobacter vibrioides (strain ATCC 19089 / CB15) OX=190650 GN=alkB PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 2.5e-13
Identity = 44/115 (38.26%), Postives = 62/115 (53.91%), Query Frame = 0

Query: 446 PWMKPNICIVNFYSQNGRLGLHQDRDESQESLEKGLPVVSFSIGDSAEFLFGDRSDTDQA 505
           P   P+ C+VN Y    R+GLHQDRDE+    +   PV+S S+GD+A F  G  +  D  
Sbjct: 114 PETPPDSCLVNLYRDGARMGLHQDRDEA----DPRFPVLSISLGDTAVFRIGGVNRKDPT 173

Query: 506 EKVILESGDILIFGGKSRHVFHGVTAIHPNTAPKALLDETNICP--GRLNLTFRQ 559
             + L SGD+    G +R  FHGV  I P +        +++ P  GR+NLT R+
Sbjct: 174 RSLRLASGDVCRLLGPARLAFHGVDRILPGS--------SSLVPGGGRINLTLRR 216

BLAST of Spg024058 vs. ExPASy Swiss-Prot
Match: B8GWW6 (Alpha-ketoglutarate-dependent dioxygenase AlkB homolog OS=Caulobacter vibrioides (strain NA1000 / CB15N) OX=565050 GN=alkB PE=3 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 2.5e-13
Identity = 44/115 (38.26%), Postives = 62/115 (53.91%), Query Frame = 0

Query: 446 PWMKPNICIVNFYSQNGRLGLHQDRDESQESLEKGLPVVSFSIGDSAEFLFGDRSDTDQA 505
           P   P+ C+VN Y    R+GLHQDRDE+    +   PV+S S+GD+A F  G  +  D  
Sbjct: 114 PETPPDSCLVNLYRDGARMGLHQDRDEA----DPRFPVLSISLGDTAVFRIGGVNRKDPT 173

Query: 506 EKVILESGDILIFGGKSRHVFHGVTAIHPNTAPKALLDETNICP--GRLNLTFRQ 559
             + L SGD+    G +R  FHGV  I P +        +++ P  GR+NLT R+
Sbjct: 174 RSLRLASGDVCRLLGPARLAFHGVDRILPGS--------SSLVPGGGRINLTLRR 216

BLAST of Spg024058 vs. ExPASy Swiss-Prot
Match: Q54N08 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44689 GN=alkB PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 6.9e-11
Identity = 35/92 (38.04%), Postives = 56/92 (60.87%), Query Frame = 0

Query: 455 VNFYSQNGRLGLHQDRDESQESLEKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGD 514
           VNFYS++  +G H   D++++ +EK  P++S S G +A FL G  +       + + SGD
Sbjct: 266 VNFYSEDSIMGGH--LDDAEQEMEK--PIISISFGSTAVFLMGAETRDIAPVPLFIRSGD 325

Query: 515 ILIFGGKSRHVFHGVTAIHPNTAPKALLDETN 547
           I+I GG+SR+ +HGV  I  N+    L+DE +
Sbjct: 326 IVIMGGRSRYCYHGVAKIVENSFDLGLIDEND 353

BLAST of Spg024058 vs. ExPASy TrEMBL
Match: A0A5D3CA69 (Oxoglutarate/iron-dependent dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold606G001030 PE=4 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 2.5e-229
Identity = 409/502 (81.47%), Postives = 437/502 (87.05%), Query Frame = 0

Query: 59  MNHGGPRYAGRGHPNNRGRSPRSADNSTYRPRHAGGEGPSVPGSSNPSGGAFRERSFHQM 118
           MN GGPRYAGRGHPNNRGRSPRSADN TYRPRHAGGEG SV GSSNP  GAFR RS HQM
Sbjct: 1   MNDGGPRYAGRGHPNNRGRSPRSADNFTYRPRHAGGEGSSVAGSSNPGSGAFRGRSSHQM 60

Query: 119 PSRNFRKQFVQKQASSSEQQQWRPINSGKDASPGAVDLQQEHKLADDMSNTSKQSLGSVA 178
            SRNFRK   QKQASSSEQ QWRP+NS KDASPGAVDL  +H   DDMSNT+KQ LGS+A
Sbjct: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSRKDASPGAVDLHLQHNSTDDMSNTNKQLLGSIA 120

Query: 179 SDSDGNLMSNTSEQLLGSIASNSDCNELSPSSAQNVSKSLHSAVERIQIREPIAEGGSCS 238
           S+SD N +SNTSEQ LG IASNSDC EL  SSAQNVSKSLHSAVERIQI+ P A  G+CS
Sbjct: 121 SNSDSNQISNTSEQRLGPIASNSDCIELLSSSAQNVSKSLHSAVERIQIQGPTAVCGNCS 180

Query: 239 TSLPYDNCNRSDAVEQELIVQVPLESCAKDEGSTRKLRE-NDISYCKDSEDKKPSVNLNP 298
            S PYDN N SD V QEL VQ  LESCAKD+ ST KL E N++    DS+DKKPSV+L+ 
Sbjct: 181 DSFPYDNRNGSDVVGQELKVQPTLESCAKDKSSTIKLGESNNVFNSMDSKDKKPSVDLDT 240

Query: 299 FDICPPKSGGIKLNPSLLAKNREKRNEMKRAMEGNSGVVLRPGMVHLKSGISLRDQVKIV 358
           FDICPPK+GG+ LNPSLLA NREKRNEMKRAM+GN+G+VLRPGMVHLK  ISLRDQ KIV
Sbjct: 241 FDICPPKTGGVTLNPSLLAMNREKRNEMKRAMDGNNGIVLRPGMVHLKGSISLRDQAKIV 300

Query: 359 KRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSSYGDVRPFDGTKPPGIPNEF 418
           K+CRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSS+YGDVRPFD TKPP +P+EF
Sbjct: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDDTKPPNLPDEF 360

Query: 419 YQLVEKAIKDSYAIMEKDSATKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 478
           YQLVEKAIKDSYAI+ KDS  KNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL
Sbjct: 361 YQLVEKAIKDSYAIIAKDSTIKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 420

Query: 479 EKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGDILIFGGKSRHVFHGVTAIHPNTA 538
           +KGLPV+SFSIGDSAEFLFGD SD DQAEKV LESGDILIFGGKSRHVFHGVTAIH NTA
Sbjct: 421 DKGLPVISFSIGDSAEFLFGDWSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA 480

Query: 539 PKALLDETNICPGRLNLTFRQY 560
           PKALL+ TN+ PGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 502

BLAST of Spg024058 vs. ExPASy TrEMBL
Match: A0A1S4E4H2 (LOW QUALITY PROTEIN: uncharacterized protein LOC103501985 OS=Cucumis melo OX=3656 GN=LOC103501985 PE=4 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 1.4e-227
Identity = 408/502 (81.27%), Postives = 436/502 (86.85%), Query Frame = 0

Query: 59  MNHGGPRYAGRGHPNNRGRSPRSADNSTYRPRHAGGEGPSVPGSSNPSGGAFRERSFHQM 118
           MN GGPRYAGRGHPNNRGRSPRSADN TYRPRHAGGEG SV GSSNP  GAFR RS HQM
Sbjct: 1   MNDGGPRYAGRGHPNNRGRSPRSADNFTYRPRHAGGEGSSVAGSSNPGSGAFRGRSSHQM 60

Query: 119 PSRNFRKQFVQKQASSSEQQQWRPINSGKDASPGAVDLQQEHKLADDMSNTSKQSLGSVA 178
            SRNFRK   QKQASSSEQ QWRP+NS KDASPGAVDL  +H   DDMSNT+KQ LGS+A
Sbjct: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSRKDASPGAVDLHLQHNSTDDMSNTNKQLLGSIA 120

Query: 179 SDSDGNLMSNTSEQLLGSIASNSDCNELSPSSAQNVSKSLHSAVERIQIREPIAEGGSCS 238
           S+SD N +SNTSEQ LG IASNSDC EL  SSAQNVSKSLHSAVERIQI+ P A  G+CS
Sbjct: 121 SNSDSNQISNTSEQRLGPIASNSDCIELLSSSAQNVSKSLHSAVERIQIQGPTAVCGNCS 180

Query: 239 TSLPYDNCNRSDAVEQELIVQVPLESCAKDEGSTRKLRE-NDISYCKDSEDKKPSVNLNP 298
            S PYDN N SD V QEL VQ  LESCAKD+ ST KL E N++    DS+DKKPSV+L+ 
Sbjct: 181 DSFPYDNRNGSDVVGQELKVQPTLESCAKDKSSTIKLGESNNVFNSMDSKDKKPSVDLDT 240

Query: 299 FDICPPKSGGIKLNPSLLAKNREKRNEMKRAMEGNSGVVLRPGMVHLKSGISLRDQVKIV 358
           FDICPPK+GG+ LNPSLLA NREK NEMKRAM+GN+G+VLRPGMVHLK  ISLRDQ KIV
Sbjct: 241 FDICPPKTGGVTLNPSLLAMNREK-NEMKRAMDGNNGIVLRPGMVHLKGSISLRDQAKIV 300

Query: 359 KRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSSYGDVRPFDGTKPPGIPNEF 418
           K+CRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSS+YGDVRPFD TKPP +P+EF
Sbjct: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDDTKPPNLPDEF 360

Query: 419 YQLVEKAIKDSYAIMEKDSATKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 478
           YQLVEKAIKDSYAI+ KDS  KNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL
Sbjct: 361 YQLVEKAIKDSYAIIAKDSTIKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 420

Query: 479 EKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGDILIFGGKSRHVFHGVTAIHPNTA 538
           +KGLPV+SFSIGDSAEFLFGDRSD DQAEKV LESGDILIFGGKSRHVFHGVTAIH  TA
Sbjct: 421 DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSKTA 480

Query: 539 PKALLDETNICPGRLNLTFRQY 560
           PKALL+ TN+ PGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 501

BLAST of Spg024058 vs. ExPASy TrEMBL
Match: A0A0A0LC72 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G651830 PE=4 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 5.2e-227
Identity = 410/536 (76.49%), Postives = 442/536 (82.46%), Query Frame = 0

Query: 25  LSLKLIKRIANDSASFGIQFPSINLFYFQFPFRIMNHGGPRYAGRGHPNNRGRSPRSADN 84
           LS KLI+R+AN SASF IQFP            IMN GGPRYAGRGHPNNRGRSPRSAD+
Sbjct: 30  LSHKLIERLANSSASFVIQFPP-----------IMNDGGPRYAGRGHPNNRGRSPRSADH 89

Query: 85  STYRPRHAGGEGPSVPGSSNPSGGAFRERSFHQMPSRNFRKQFVQKQASSSEQQQWRPIN 144
            TYRPRHAGGEG SV GSSNP  GAFR RS HQM SRNFRK   QKQASSSEQ QWRP+N
Sbjct: 90  FTYRPRHAGGEGSSVAGSSNPGSGAFRGRSSHQMSSRNFRKPVGQKQASSSEQWQWRPLN 149

Query: 145 SGKDASPGAVDLQQEHKLADDMSNTSKQSLGSVASDSDGNLMSNTSEQLLGSIASNSDCN 204
           SGKDASPGAVDLQ +H   DDMSN +K                    QLL SIASNSDC 
Sbjct: 150 SGKDASPGAVDLQLQHNSTDDMSNNNK--------------------QLLESIASNSDCI 209

Query: 205 ELSPSSAQNVSKSLHSAVERIQIREPIAEGGSCSTSLPYDNCNRSDAVEQELIVQVPLES 264
           ELS SSAQNVSKSLHSAVERI ++ P A  GS   S PYDNCNRSD V QEL VQ  L+S
Sbjct: 210 ELSSSSAQNVSKSLHSAVERIHVQGPTAVCGSYGDSFPYDNCNRSDVVGQELKVQPSLKS 269

Query: 265 CAKDEGSTRKL-RENDISYCKDSEDKKPSVNLNPFDICPPKSGGIKLNPSLLAKNREKRN 324
           CAKDE  T +L + ND+    DS+DKKPSV+L+ FDICPPK+GG+ LNPSLLA NREKRN
Sbjct: 270 CAKDESFTIQLGKSNDVFNSTDSKDKKPSVDLDSFDICPPKTGGVMLNPSLLAMNREKRN 329

Query: 325 EMKRAMEGNSGVVLRPGMVHLKSGISLRDQVKIVKRCRDLGIGAGGFYQPGYREGGKLHL 384
           EM+RAMEGN+G+VLRPGMVHLK GIS+RDQ KIVK+CRDLGIGAGGFYQPGYREGGKLHL
Sbjct: 330 EMRRAMEGNNGIVLRPGMVHLKGGISVRDQAKIVKKCRDLGIGAGGFYQPGYREGGKLHL 389

Query: 385 KMMCLGKNWDPDSSSYGDVRPFDGTKPPGIPNEFYQLVEKAIKDSYAIMEKDSATKNPER 444
           KMMCLGKNWDPDSS+YGD+RPFD TKPP +P+EFYQLVEKAIKDSYAIM +DS  KNPER
Sbjct: 390 KMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEFYQLVEKAIKDSYAIMAEDSTIKNPER 449

Query: 445 VLPWMKPNICIVNFYSQNGRLGLHQDRDESQESLEKGLPVVSFSIGDSAEFLFGDRSDTD 504
           VLPWMKP+ICIVNFYSQNGRLGLHQDRDESQESL+KGLPV+SFSIGDSAEFLFGDRSD D
Sbjct: 450 VLPWMKPDICIVNFYSQNGRLGLHQDRDESQESLDKGLPVISFSIGDSAEFLFGDRSDVD 509

Query: 505 QAEKVILESGDILIFGGKSRHVFHGVTAIHPNTAPKALLDETNICPGRLNLTFRQY 560
           QAEKV LESGDILIFGGKSRHVFHGVTAIH NTAPKALL+ TN+ PGRLNLTFRQY
Sbjct: 510 QAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTAPKALLEATNLRPGRLNLTFRQY 534

BLAST of Spg024058 vs. ExPASy TrEMBL
Match: A0A6J1GV59 (uncharacterized protein LOC111457830 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457830 PE=4 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 3.8e-222
Identity = 404/502 (80.48%), Postives = 430/502 (85.66%), Query Frame = 0

Query: 59  MNHGGPRYAGRGHPNNRGRSPRSADNSTYRPRHAGGEGPSVPGSSNPSGGAFRERSFHQM 118
           MN GG RY GRGHPNNRGR PRSADN TYRPRHAGGEGPS  GSSNP  GAF++RS H  
Sbjct: 1   MNDGGHRYGGRGHPNNRGRFPRSADNFTYRPRHAGGEGPSAAGSSNPRNGAFKDRSSH-- 60

Query: 119 PSRNFRKQFVQKQASSSEQQQWRPINSGKDASPGAVDLQQEHKLADDMSNTSKQSLGSVA 178
           PSRN R  F QKQA  SEQ QWRP+NSGKDASPGAVDLQQ+H  ADD++   KQ +GS++
Sbjct: 61  PSRNSRNTFGQKQALGSEQWQWRPLNSGKDASPGAVDLQQDHNTADDIA--PKQLIGSIS 120

Query: 179 SDSDGNLMSNTSEQLLGSIASNSDCNELSPSSAQNVSKSLHSAVERIQIREPIAEGGSCS 238
           S+SDGN MSNTSEQLLGSIAS SD NE SP SAQNVSKSLHSAVERIQIREP AEG  C 
Sbjct: 121 SNSDGNDMSNTSEQLLGSIASKSDGNEHSPCSAQNVSKSLHSAVERIQIREPTAEGERCD 180

Query: 239 TSLPYDNCNRSDAVEQELIVQVPLESCAKDEGSTRKLRE-NDISYCKDSEDKKPSVNLNP 298
            S PYD+C RSDA  QEL+VQ        D+ +T KLRE N++S CKDS+DKKP  NL  
Sbjct: 181 DSFPYDSCKRSDAGGQELMVQ--------DQTATIKLRESNNVSDCKDSKDKKPLGNLEI 240

Query: 299 FDICPPKSGGIKLNPSLLAKNREKRNEMKRAMEGNSGVVLRPGMVHLKSGISLRDQVKIV 358
           FDICPPKSG + LNPSLL+KNREKRNEMKRAMEGN+G VLRPGMVHLKSGISL DQVKIV
Sbjct: 241 FDICPPKSGVVTLNPSLLSKNREKRNEMKRAMEGNNGNVLRPGMVHLKSGISLSDQVKIV 300

Query: 359 KRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSSYGDVRPFDGTKPPGIPNEF 418
           K+CRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSS YGDVRPFD T PP +P EF
Sbjct: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSKYGDVRPFDNTTPPNMPVEF 360

Query: 419 YQLVEKAIKDSYAIMEKDSATKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 478
           Y+LVEKAIKDSYA+M KDS TKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL
Sbjct: 361 YKLVEKAIKDSYAVMGKDSNTKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 420

Query: 479 EKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGDILIFGGKSRHVFHGVTAIHPNTA 538
           EKGLPVVSFSIGDSAEFLFGD SD DQAEKV LESGDILIFGGKSRHVFHGVTAIH NTA
Sbjct: 421 EKGLPVVSFSIGDSAEFLFGDWSDIDQAEKVTLESGDILIFGGKSRHVFHGVTAIHQNTA 480

Query: 539 PKALLDETNICPGRLNLTFRQY 560
           PKALL+ TN+ PGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 490

BLAST of Spg024058 vs. ExPASy TrEMBL
Match: A0A6J1IWW9 (uncharacterized protein LOC111479925 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479925 PE=4 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 1.1e-221
Identity = 403/502 (80.28%), Postives = 431/502 (85.86%), Query Frame = 0

Query: 59  MNHGGPRYAGRGHPNNRGRSPRSADNSTYRPRHAGGEGPSVPGSSNPSGGAFRERSFHQM 118
           MN GG RY GRGHPNNRGR PRSAD+ TYRPRHAGGEGPSV GSSNP  GAF++RS H  
Sbjct: 1   MNDGGHRYGGRGHPNNRGRFPRSADSFTYRPRHAGGEGPSVAGSSNPRNGAFKDRSSH-- 60

Query: 119 PSRNFRKQFVQKQASSSEQQQWRPINSGKDASPGAVDLQQEHKLADDMSNTSKQSLGSVA 178
           PSRN R  F QKQA  SEQ QWRP+NSGKD+SPGAVDLQQ H  ADD++   KQ +GS++
Sbjct: 61  PSRNSRNTFGQKQALGSEQWQWRPLNSGKDSSPGAVDLQQYHNTADDIA--PKQLIGSIS 120

Query: 179 SDSDGNLMSNTSEQLLGSIASNSDCNELSPSSAQNVSKSLHSAVERIQIREPIAEGGSCS 238
           S+SDGN MSNTSEQLLGSIAS SD NE SPSSAQNVSKSLHSAVERIQIREP AEG  C 
Sbjct: 121 SNSDGNHMSNTSEQLLGSIASKSDGNEHSPSSAQNVSKSLHSAVERIQIREPTAEGERCD 180

Query: 239 TSLPYDNCNRSDAVEQELIVQVPLESCAKDEGSTRKLRE-NDISYCKDSEDKKPSVNLNP 298
            S PY +C RSDA  QE +VQ        D+ +T KLRE N++S CKDS+DKKPS NL  
Sbjct: 181 DSFPYHSCKRSDAAGQEPMVQ--------DQTATIKLRESNNVSDCKDSKDKKPSGNLEI 240

Query: 299 FDICPPKSGGIKLNPSLLAKNREKRNEMKRAMEGNSGVVLRPGMVHLKSGISLRDQVKIV 358
           FDICPPKSG + LNPSLL+KNREKRNEMKRAMEGN+G VLRPGMVHLKSGISL DQVKIV
Sbjct: 241 FDICPPKSGVVTLNPSLLSKNREKRNEMKRAMEGNNGNVLRPGMVHLKSGISLSDQVKIV 300

Query: 359 KRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSSYGDVRPFDGTKPPGIPNEF 418
           KRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSS+YGDVRPFD T PP +P EF
Sbjct: 301 KRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDNTTPPNMPVEF 360

Query: 419 YQLVEKAIKDSYAIMEKDSATKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 478
           Y+LVEKAIKDSYA++ KDS TKNPERVLPWMKPNICIVNFY QNGRLGLHQDRDESQESL
Sbjct: 361 YKLVEKAIKDSYAVLGKDSNTKNPERVLPWMKPNICIVNFYLQNGRLGLHQDRDESQESL 420

Query: 479 EKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGDILIFGGKSRHVFHGVTAIHPNTA 538
           EKGLPVVSFSIGDSAEFLFGDRSD DQAEKV LESGDILIFGGKSRHVFHGVTAIH NTA
Sbjct: 421 EKGLPVVSFSIGDSAEFLFGDRSDIDQAEKVTLESGDILIFGGKSRHVFHGVTAIHQNTA 480

Query: 539 PKALLDETNICPGRLNLTFRQY 560
           PKALL+ TN+ PGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 490

BLAST of Spg024058 vs. TAIR 10
Match: AT3G14160.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 330.1 bits (845), Expect = 3.4e-90
Identity = 221/518 (42.66%), Postives = 286/518 (55.21%), Query Frame = 0

Query: 59  MNHGGPRYAGRGHPNNRGRSPRS-------------ADNSTYRPRHAGGEGPSVPGSS-- 118
           MNH   R +G GH   RGRS  S              D+S  R R + G+G S    S  
Sbjct: 1   MNHSEARGSG-GHKGYRGRSQASEQWVPVAADDKSLEDSSGNRVRGSQGKGRSRTSWSPR 60

Query: 119 NPSGGAFRERSFHQMPSRNFRKQFVQKQASSSEQQQWRPINSGKDASPGAVDLQQEHK-L 178
           N  G    E S  Q         FV+K                         +QQ+ K L
Sbjct: 61  NSYGRGNDEHSPVQAYVNKSNVGFVEK------------------------GVQQDRKSL 120

Query: 179 ADDMSNTSKQSLGSVASDSDGNLMSNTSEQLLGSIASNSDCNELSPSSAQNVSKSLHSAV 238
            D + +T +   G+ A D+   L S ++    GS  S S+C +      ++ +K     V
Sbjct: 121 EDGIGSTKQPDDGAAAGDNKAVLQSKSTNVSGGSFVS-SECED------KDGAKMYCDLV 180

Query: 239 ERIQIREPIAEGGSCSTSLPYDNCNRSDAVEQ-ELIVQVPLESCAKDEGSTRKLRENDIS 298
            R+              +L       S  V++ EL      +S  K +G+     E+   
Sbjct: 181 NRVN-----------DVTLSCQESVSSTVVQKVELSSVEDQKSAPKADGAGNSSNESSTR 240

Query: 299 YCKDSEDKKPSVNLNPFDICPPKSGGIKLNPSLLAKNREKRNEMKRAMEGNSGVVLRPGM 358
           +               FDI   K  GI L P+LL  +REK    K+A +G SG V+RPGM
Sbjct: 241 H---------------FDIFLEKK-GIVLKPNLLVLSREK----KKAAKGYSGTVIRPGM 300

Query: 359 VHLKSGISLRDQVKIVKRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSSYGD 418
           V LK+ +S+ DQV IV +CR LG+G GGFYQPGYR+  KLHLKMMCLGKNWDP++S YG+
Sbjct: 301 VLLKNYLSINDQVMIVNKCRRLGLGEGGFYQPGYRDEAKLHLKMMCLGKNWDPETSRYGE 360

Query: 419 VRPFDGTKPPGIPNEFYQLVEKAIKDSYAIMEKDSATKNPERVLPWMKPNICIVNFYSQN 478
            RPFDG+  P IP EF Q VEKA+K+S ++   +S        +P+M P+ICIVNFYS  
Sbjct: 361 TRPFDGSTAPRIPAEFNQFVEKAVKESQSLAASNSKQTKGGDEIPFMLPDICIVNFYSST 420

Query: 479 GRLGLHQDRDESQESLEKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGDILIFGGK 538
           GRLGLHQD+DES+ S+ KGLPVVSFSIGDSAEFL+GD+ D D+AE + LESGD+L+FGG+
Sbjct: 421 GRLGLHQDKDESENSIRKGLPVVSFSIGDSAEFLYGDQRDEDKAETLTLESGDVLLFGGR 455

Query: 539 SRHVFHGVTAIHPNTAPKALLDETNICPGRLNLTFRQY 560
           SR VFHGV +I  +TAPKALL ET++ PGRLNLTFRQY
Sbjct: 481 SRKVFHGVRSIRKDTAPKALLQETSLRPGRLNLTFRQY 455

BLAST of Spg024058 vs. TAIR 10
Match: AT5G01780.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 288.5 bits (737), Expect = 1.2e-77
Identity = 152/327 (46.48%), Postives = 210/327 (64.22%), Query Frame = 0

Query: 235 GSCSTSLPYDNCNRSDAVEQELIVQVPLESCAKDEGSTRKLRENDISYCKDSEDKKPSVN 294
           GS ++ + +D+ N S + +      + +        S  K R+      KD         
Sbjct: 76  GSPTSMVHFDSTNPSSSSKSSQSQNLKIRKVRNHRNSGFKSRDQSPQRIKDPP------- 135

Query: 295 LNPFDICPP--KSGGIKLNPSLLAKNREKRNEMKRAMEGNSGVVLRPGMVHLKSGISLRD 354
             PFDIC    +     +   +LA    +    +     N   V+RPGMV LK  ++   
Sbjct: 136 --PFDICSSVLERNDTSIKDWILADETNR----ETVEVSNKHKVIRPGMVLLKDFLTPDI 195

Query: 355 QVKIVKRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSSYGDVRPFDGTKPPG 414
           QV IVK CR+LG+   GFYQPGY  G KLHL+MMCLG+NWDP  + Y      D +K P 
Sbjct: 196 QVDIVKTCRELGVKPTGFYQPGYSVGSKLHLQMMCLGRNWDP-QTKYRKNTDID-SKAPE 255

Query: 415 IPNEFYQLVEKAIKDSYAIMEKDSATKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDE 474
           IP  F  LVEKAI++++A+++++S T++ ER+LP M P+ICIVNFYS+ GRLGLHQDRDE
Sbjct: 256 IPVTFNVLVEKAIREAHALIDRESGTEDAERILPVMSPDICIVNFYSETGRLGLHQDRDE 315

Query: 475 SQESLEKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGDILIFGGKSRHVFHGVTAI 534
           S+ES+ +GLP+VSFSIGDSAEFL+G++ D ++A+ VILESGD+LIFGG+SR +FHGV +I
Sbjct: 316 SEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVILESGDVLIFGGESRMIFHGVKSI 375

Query: 535 HPNTAPKALLDETNICPGRLNLTFRQY 560
            PN+AP +LL+E+ +  GRLNLTFR +
Sbjct: 376 IPNSAPMSLLNESKLRTGRLNLTFRHF 387

BLAST of Spg024058 vs. TAIR 10
Match: AT3G14140.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 277.3 bits (708), Expect = 2.7e-74
Identity = 139/265 (52.45%), Postives = 184/265 (69.43%), Query Frame = 0

Query: 296 NPFDICPPKSGGIKLNPSLLAKNREKRNEMKRAMEGNSGVVLRPGMVHLKSGISLRDQVK 355
           +PFDI   K   ++L PS L  NREK    K+A +G SG+V+RPGMV LK+ +S+ +QV 
Sbjct: 202 SPFDIFLKKK-VMRLKPSFLELNREK----KKAAKGFSGIVIRPGMVLLKNYLSINNQVM 261

Query: 356 IVKRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSSYGDVRPFDGTKPPGIPN 415
           IV +CR LG+G GGFYQPG+++GG LHLKMMCLGKNWD  +  YG++RP DG+ PP IP 
Sbjct: 262 IVNKCRQLGLGEGGFYQPGFQDGGLLHLKMMCLGKNWDCQTRRYGEIRPIDGSVPPRIPV 321

Query: 416 EFYQLVEKAIKDSYAIMEKDSATKNPERVLPWMKPNICIVNFYSQNGRLGLHQ------- 475
           EF QLVEKAIK+S +++  +S        +P + P+IC+VNFY+  G+LGLHQ       
Sbjct: 322 EFSQLVEKAIKESKSLVATNSNETKGGDEIPLLLPDICVVNFYTSTGKLGLHQVSVYDKT 381

Query: 476 --------------DRDESQESLEKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGD 535
                         D+ ES++SL KGLP+VSFSIGDSAEFL+GD+ D D+A+ +ILESGD
Sbjct: 382 SFDFLKYKGGYLNTDKGESKKSLRKGLPIVSFSIGDSAEFLYGDQKDVDKADTLILESGD 441

Query: 536 ILIFGGKSRHVFHGVTAIHPNTAPK 540
           +LIFG +SR+VFHGV +I     P+
Sbjct: 442 VLIFGERSRNVFHGVRSIRKILPPR 461

BLAST of Spg024058 vs. TAIR 10
Match: AT5G01780.2 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 276.2 bits (705), Expect = 5.9e-74
Identity = 180/455 (39.56%), Postives = 251/455 (55.16%), Query Frame = 0

Query: 113 RSFHQMPSRNFRKQFVQKQASSS--EQQQWRPINSGKDASPGAV-DLQQEHKLADDMSNT 172
           RSFH         +     AS +        PI     AS  ++ D +++       S T
Sbjct: 7   RSFHLTSRHQIVLRLCSPSASRTIMSSSPHSPIPKTHTASLDSLNDYEEQFPPLTGGSKT 66

Query: 173 SKQSLGSVASDS---DGNLMSNTSEQLLGSIASNSDCNELSPSSAQNVSKSLHSAVERIQ 232
            K  LGS    +      L + TS +   S+  N  C        +  S+ LH       
Sbjct: 67  KKFYLGSTNPSTPCQSSQLQNWTSGKDALSLQRNLGCKN---RRRRRASRFLHEESNGTT 126

Query: 233 IREPIAEGGSCSTSLPYDNCNRSDAVEQELIVQVPLESCAKDEGSTRKLRENDISYCKDS 292
             E  A  GS ++ + +D+ N S + +      + +        S  K R+      KD 
Sbjct: 127 F-EVGAGIGSPTSMVHFDSTNPSSSSKSSQSQNLKIRKVRNHRNSGFKSRDQSPQRIKDP 186

Query: 293 EDKKPSVNLNPFDICPP--KSGGIKLNPSLLAKNREKRNEMKRAMEGNSGVVLRPGMVHL 352
                     PFDIC    +     +   +LA    +    +     N   V+RPGMV L
Sbjct: 187 P---------PFDICSSVLERNDTSIKDWILADETNR----ETVEVSNKHKVIRPGMVLL 246

Query: 353 KSGISLRDQVKIVKRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSSYGDVRP 412
           K  ++   QV IVK CR+LG+   GFYQPGY  G KLHL+MMCLG+NWDP  + Y     
Sbjct: 247 KDFLTPDIQVDIVKTCRELGVKPTGFYQPGYSVGSKLHLQMMCLGRNWDP-QTKYRKNTD 306

Query: 413 FDGTKPPGIPNEFYQLVEKAIKDSYAIMEKDSATKNPERVLPWMKPNICIVNFYSQNGRL 472
            D +K P IP  F  LVEKAI++++A+++++S T++ ER+LP M P+ICIVNFYS+ GRL
Sbjct: 307 ID-SKAPEIPVTFNVLVEKAIREAHALIDRESGTEDAERILPVMSPDICIVNFYSETGRL 366

Query: 473 GLHQDRDESQESLEKGLPVVSFSIGDSAEFLFGDRSDTDQAEKVILESGDILIFGGKSRH 532
           GLHQDRDES+ES+ +GLP+VSFSIGDSAEFL+G++ D ++A+ VILESGD+LIFGG+SR 
Sbjct: 367 GLHQDRDESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVILESGDVLIFGGESRM 426

Query: 533 VFHGVTAIHPNTAPKALLDETNICPGRLNLTFRQY 560
           +FHGV +I PN+AP +LL+E+ +  GRLNLTFR +
Sbjct: 427 IFHGVKSIIPNSAPMSLLNESKLRTGRLNLTFRHF 442

BLAST of Spg024058 vs. TAIR 10
Match: AT1G11780.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 60.5 bits (145), Expect = 5.1e-09
Identity = 31/84 (36.90%), Postives = 45/84 (53.57%), Query Frame = 0

Query: 449 KPNICIVNFYSQNGRLGLHQDRDESQESLEKGLPVVSFSIGDSAEFLFGDRSDTDQAEKV 508
           +P   IVN++     LG H D  E+  S     P+VS S+G  A FL G +S  D    +
Sbjct: 225 RPEGAIVNYFGIGDTLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSKDDPPHAM 284

Query: 509 ILESGDILIFGGKSRHVFHGVTAI 533
            L SGD+++  G++R  FHG+  I
Sbjct: 285 YLRSGDVVLMAGEARECFHGIPRI 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875730.11.2e-23382.67uncharacterized protein LOC120068103 isoform X1 [Benincasa hispida][more]
KAA0058530.15.1e-22981.47Oxoglutarate/iron-dependent dioxygenase [Cucumis melo var. makuwa] >TYK07216.1 O... [more]
XP_016903133.12.8e-22781.27PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103501985 [Cucumis me... [more]
KAG6581809.11.2e-22280.68hypothetical protein SDJN03_21811, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7018258.11.6e-22280.68alkB [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
P050507.9e-1530.00Alpha-ketoglutarate-dependent dioxygenase AlkB OS=Escherichia coli (strain K12) ... [more]
P374628.8e-1436.84Alpha-ketoglutarate-dependent dioxygenase AlkB OS=Salmonella typhimurium (strain... [more]
P0CAT72.5e-1338.26Alpha-ketoglutarate-dependent dioxygenase AlkB homolog OS=Caulobacter vibrioides... [more]
B8GWW62.5e-1338.26Alpha-ketoglutarate-dependent dioxygenase AlkB homolog OS=Caulobacter vibrioides... [more]
Q54N086.9e-1138.04Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44... [more]
Match NameE-valueIdentityDescription
A0A5D3CA692.5e-22981.47Oxoglutarate/iron-dependent dioxygenase OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S4E4H21.4e-22781.27LOW QUALITY PROTEIN: uncharacterized protein LOC103501985 OS=Cucumis melo OX=365... [more]
A0A0A0LC725.2e-22776.49Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G... [more]
A0A6J1GV593.8e-22280.48uncharacterized protein LOC111457830 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1IWW91.1e-22180.28uncharacterized protein LOC111479925 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT3G14160.13.4e-9042.662-oxoglutarate-dependent dioxygenase family protein [more]
AT5G01780.11.2e-7746.482-oxoglutarate-dependent dioxygenase family protein [more]
AT3G14140.12.7e-7452.452-oxoglutarate-dependent dioxygenase family protein [more]
AT5G01780.25.9e-7439.562-oxoglutarate-dependent dioxygenase family protein [more]
AT1G11780.15.1e-0936.90oxidoreductase, 2OG-Fe(II) oxygenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 552..559
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 167..188
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 126..145
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 65..112
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 126..152
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 165..188
NoneNo IPR availablePANTHERPTHR16557:SF92OG-FE(II) OXYGENASE FAMILY PROTEINcoord: 116..559
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 337..559
IPR027450Alpha-ketoglutarate-dependent dioxygenase AlkB-likePFAMPF135322OG-FeII_Oxy_2coord: 340..557
e-value: 7.3E-42
score: 143.8
IPR037151Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamilyGENE3D2.60.120.590coord: 335..559
e-value: 2.9E-52
score: 179.3
IPR004574Alkylated DNA repair protein AlkBPANTHERPTHR16557ALKYLATED DNA REPAIR PROTEIN ALKB-RELATEDcoord: 116..559
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 449..559
score: 10.048603

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg024058.1Spg024058.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006281 DNA repair
molecular_function GO:0051213 dioxygenase activity