Spg023966 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg023966
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Delta(8)-fatty-acid desaturase
Location	scaffold13: 12524618 .. 12525961 (-)
RNA-Seq Expression	Spg023966
Synteny	Spg023966

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGCCAAGAAGAAGTACATTTCCTCTGAGGAGCTTCTCAAGCACAACAAACCAGGAGATCTGTGGATCTCCATTCAGGGTAAGGTCTATAATGTCTCAGATTGGGCTAAGGAACATCCTGGTGGCGATACGCCTTTTCTCAATTTGGCGGGTCATGATGTTACTGATGCATTCATCGCCTATCATCCTGGCACCGCTTGGGCATATCTCGACCGCTTGTTCACTGGATTTCATCTCCAGGATTTCCAGGTCTCCGAGATTTCTAAGGACTACAGGCGCCTTGCTTCTGAGTTTTCCAAACAAGGTTTGTTCGAGAAGAAGGGGCATGTTACCATGTATTCTTTGATTTCTGTTGCTCTCATGTTATTTCTTGTGGTTTATGGGGTTGTCAAATCTGAGAGTGTTTTGGTTCATCTGGCTTCTGCTATGCTTTTGGGTATGCTTTGGATTCAAAGTGCTTATGTGGGTCATGATTCTGGCCATTCTAATGTAATGACCACCCGTGGATTTAACAAATTGGCTCAAATTCTCTCTGGGAATTGCTTGACTGGGATTAGTATAGCCTGGTGGAAATGGACTCATAATGCTCATCATATTTCCTGCAATAGCCTTGACCACGATCCAGATCTTCAGCACATCCCTGTTTTTGCTGTTTCGGCTAATTTCTTCCGGTCTTTGACATCTCATTTCTATGGTAGGAAGTTGAAGTTTGATTCCTTGGCTAGATTCTTCGTTAGTTACCAGCATTTCACATTTTACCCTGTAATGTGTGTGGCTAGAGTGAATCTCTTTGTCCAGACATTGTTGCTCTTGTTTTCCACTAGAAAAGTCCCTGATAGAGCATTGAACATAATGGGGATTCTTGTGTTTTACACTTGGTTTCCTCTTCTGGTTTCCTATCTCCCCAATTGGCCTGAGAGGTTCATGTTTGTTCTTGCAAGTTTTGCTGTCACATCTCTGCAGCACATCCAATTCTGCCTCAACCATTTCTCTGCTAATGTTTATGTTGGGAAGCCCACTGGGAATGACTGGTTTGAGAAGCAAACCAGTGGGACTTTGGATATCGATTGTTCTTCTTGGATGGACTGGTTTTTTGGTGGATTGCAGTTTCAGCTTGAGCATCATTTGTTCCCAAGGATGCCCAGATGCCAACTGAGGAAAATCTCTCCAATTGTTGTGGAGCTTTGCAAGAAGCACAATCTTCCTTACAGAAGCATGTCATTCTGGGATGCTAATGTCCGCACCATTAGAACTCTTAGAACAGCTGCTTTGCAGGCTAGAGACTTGACCAACCCTGTTCCTAAGAACTTGCTATGGGAAGCTGTGAACACCCATGGATGA

mRNA sequence

ATGGAGGCCAAGAAGAAGTACATTTCCTCTGAGGAGCTTCTCAAGCACAACAAACCAGGAGATCTGTGGATCTCCATTCAGGGTAAGGTCTATAATGTCTCAGATTGGGCTAAGGAACATCCTGGTGGCGATACGCCTTTTCTCAATTTGGCGGGTCATGATGTTACTGATGCATTCATCGCCTATCATCCTGGCACCGCTTGGGCATATCTCGACCGCTTGTTCACTGGATTTCATCTCCAGGATTTCCAGGTCTCCGAGATTTCTAAGGACTACAGGCGCCTTGCTTCTGAGTTTTCCAAACAAGGTTTGTTCGAGAAGAAGGGGCATGTTACCATGTATTCTTTGATTTCTGTTGCTCTCATGTTATTTCTTGTGGTTTATGGGGTTGTCAAATCTGAGAGTGTTTTGGTTCATCTGGCTTCTGCTATGCTTTTGGGTATGCTTTGGATTCAAAGTGCTTATGTGGGTCATGATTCTGGCCATTCTAATGTAATGACCACCCGTGGATTTAACAAATTGGCTCAAATTCTCTCTGGGAATTGCTTGACTGGGATTAGTATAGCCTGGTGGAAATGGACTCATAATGCTCATCATATTTCCTGCAATAGCCTTGACCACGATCCAGATCTTCAGCACATCCCTGTTTTTGCTGTTTCGGCTAATTTCTTCCGGTCTTTGACATCTCATTTCTATGGTAGGAAGTTGAAGTTTGATTCCTTGGCTAGATTCTTCGTTAGTTACCAGCATTTCACATTTTACCCTGTAATGTGTGTGGCTAGAGTGAATCTCTTTGTCCAGACATTGTTGCTCTTGTTTTCCACTAGAAAAGTCCCTGATAGAGCATTGAACATAATGGGGATTCTTGTGTTTTACACTTGGTTTCCTCTTCTGGTTTCCTATCTCCCCAATTGGCCTGAGAGGTTCATGTTTGTTCTTGCAAGTTTTGCTGTCACATCTCTGCAGCACATCCAATTCTGCCTCAACCATTTCTCTGCTAATGTTTATGTTGGGAAGCCCACTGGGAATGACTGGTTTGAGAAGCAAACCAGTGGGACTTTGGATATCGATTGTTCTTCTTGGATGGACTGGTTTTTTGGTGGATTGCAGTTTCAGCTTGAGCATCATTTGTTCCCAAGGATGCCCAGATGCCAACTGAGGAAAATCTCTCCAATTGTTGTGGAGCTTTGCAAGAAGCACAATCTTCCTTACAGAAGCATGTCATTCTGGGATGCTAATGTCCGCACCATTAGAACTCTTAGAACAGCTGCTTTGCAGGCTAGAGACTTGACCAACCCTGTTCCTAAGAACTTGCTATGGGAAGCTGTGAACACCCATGGATGA

Coding sequence (CDS)

ATGGAGGCCAAGAAGAAGTACATTTCCTCTGAGGAGCTTCTCAAGCACAACAAACCAGGAGATCTGTGGATCTCCATTCAGGGTAAGGTCTATAATGTCTCAGATTGGGCTAAGGAACATCCTGGTGGCGATACGCCTTTTCTCAATTTGGCGGGTCATGATGTTACTGATGCATTCATCGCCTATCATCCTGGCACCGCTTGGGCATATCTCGACCGCTTGTTCACTGGATTTCATCTCCAGGATTTCCAGGTCTCCGAGATTTCTAAGGACTACAGGCGCCTTGCTTCTGAGTTTTCCAAACAAGGTTTGTTCGAGAAGAAGGGGCATGTTACCATGTATTCTTTGATTTCTGTTGCTCTCATGTTATTTCTTGTGGTTTATGGGGTTGTCAAATCTGAGAGTGTTTTGGTTCATCTGGCTTCTGCTATGCTTTTGGGTATGCTTTGGATTCAAAGTGCTTATGTGGGTCATGATTCTGGCCATTCTAATGTAATGACCACCCGTGGATTTAACAAATTGGCTCAAATTCTCTCTGGGAATTGCTTGACTGGGATTAGTATAGCCTGGTGGAAATGGACTCATAATGCTCATCATATTTCCTGCAATAGCCTTGACCACGATCCAGATCTTCAGCACATCCCTGTTTTTGCTGTTTCGGCTAATTTCTTCCGGTCTTTGACATCTCATTTCTATGGTAGGAAGTTGAAGTTTGATTCCTTGGCTAGATTCTTCGTTAGTTACCAGCATTTCACATTTTACCCTGTAATGTGTGTGGCTAGAGTGAATCTCTTTGTCCAGACATTGTTGCTCTTGTTTTCCACTAGAAAAGTCCCTGATAGAGCATTGAACATAATGGGGATTCTTGTGTTTTACACTTGGTTTCCTCTTCTGGTTTCCTATCTCCCCAATTGGCCTGAGAGGTTCATGTTTGTTCTTGCAAGTTTTGCTGTCACATCTCTGCAGCACATCCAATTCTGCCTCAACCATTTCTCTGCTAATGTTTATGTTGGGAAGCCCACTGGGAATGACTGGTTTGAGAAGCAAACCAGTGGGACTTTGGATATCGATTGTTCTTCTTGGATGGACTGGTTTTTTGGTGGATTGCAGTTTCAGCTTGAGCATCATTTGTTCCCAAGGATGCCCAGATGCCAACTGAGGAAAATCTCTCCAATTGTTGTGGAGCTTTGCAAGAAGCACAATCTTCCTTACAGAAGCATGTCATTCTGGGATGCTAATGTCCGCACCATTAGAACTCTTAGAACAGCTGCTTTGCAGGCTAGAGACTTGACCAACCCTGTTCCTAAGAACTTGCTATGGGAAGCTGTGAACACCCATGGATGA

Protein sequence

MEAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFIAYHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVALMLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSGNCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDSLARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVSYLPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSSWMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTLRTAALQARDLTNPVPKNLLWEAVNTHG

Homology

[Close]

BLAST of Spg023966 vs. NCBI nr
Match: XP_023006577.1 (delta(8)-fatty-acid desaturase 2-like [Cucurbita maxima] >XP_023006578.1 delta(8)-fatty-acid desaturase 2-like [Cucurbita maxima])

HSP 1 Score: 891.0 bits (2301), Expect = 4.4e-255
Identity = 422/447 (94.41%), Postives = 437/447 (97.76%), Query Frame = 0


Query: 1   MEAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFI 60
           MEA+KKYI+SEELLKHNKPGDLWISIQGKVYNV+DWAKEHPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEAEKKYITSEELLKHNKPGDLWISIQGKVYNVTDWAKEHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DF VSE+SKDYRRLASEFSKQGLFEKKGHVTMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLEDFHVSEVSKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 121 LMLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSG 180
           +MLFLVVYGVVKSESVLVHL SAM+LGMLWIQSAYVGHDSGHS VM++RGFNKLAQILSG
Sbjct: 121 IMLFLVVYGVVKSESVLVHLGSAMILGMLWIQSAYVGHDSGHSTVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FF+SLTSHFYGR LKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFKSLTSHFYGRTLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVS 300
           LARFF+SYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVF+TWFPLLVS
Sbjct: 241 LARFFISYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSS 360
           YLPNWPER MFVLASFAVTSLQHIQFCLNHFSANVYVGKP GNDWFEKQT GTLDI CSS
Sbjct: 301 YLPNWPERVMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTKGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRS+SFWDANVRTI TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTINTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVP+NLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPQNLLWEAVNTHG 447

[Close]

BLAST of Spg023966 vs. NCBI nr
Match: XP_008458161.1 (PREDICTED: delta(8)-fatty-acid desaturase 2 [Cucumis melo] >TYK14028.1 delta(8)-fatty-acid desaturase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 890.6 bits (2300), Expect = 5.7e-255
Identity = 423/447 (94.63%), Postives = 438/447 (97.99%), Query Frame = 0


Query: 1   MEAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFI 60
           ME +KKYI+SEELLKHNKPGDLWISIQGKVYNV++WAK HPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DFQVSEISKDYR+LASEFSKQGLFEKKGHV MYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120

Query: 121 LMLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSG 180
           +M+FLVVYGV+KSESVLVHL  AM+LGMLWIQSAYVGHDSGHSNVM++RGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSA FFR+LTSHFYGRKLKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVF+TWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSS 360
           YLPNWPER MFVLASFAV SLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDI CSS
Sbjct: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRS+SFWDANVRTIRTL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Spg023966 vs. NCBI nr
Match: XP_022959210.1 (delta(8)-fatty-acid desaturase 2-like [Cucurbita moschata] >XP_022959211.1 delta(8)-fatty-acid desaturase 2-like [Cucurbita moschata] >KAG7013765.1 Delta(8)-fatty-acid desaturase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 889.8 bits (2298), Expect = 9.7e-255
Identity = 421/447 (94.18%), Postives = 437/447 (97.76%), Query Frame = 0


Query: 1   MEAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFI 60
           MEA+KKYI+SEELLKHNKPGDLWISIQGKVYNV+DWAKEHPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEAEKKYITSEELLKHNKPGDLWISIQGKVYNVTDWAKEHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DF VSE+SKDYRRLASEFS+QGLFEKKGHVTMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLEDFHVSEVSKDYRRLASEFSRQGLFEKKGHVTMYSLISVA 120

Query: 121 LMLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSG 180
           +MLFLVVYGVVKSESVLVHL SAM+LGMLWIQSAYVGHDSGHS VM++RGFNKLAQILSG
Sbjct: 121 IMLFLVVYGVVKSESVLVHLGSAMILGMLWIQSAYVGHDSGHSTVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FF+SLTSHFYGR LKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFKSLTSHFYGRTLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVS 300
           LARFF+SYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVF+TWFPLLVS
Sbjct: 241 LARFFISYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSS 360
           YLPNWPER MFVLASFAVTSLQHIQFCLNHFSANVYVGKP GNDWFEKQT GTLDI CSS
Sbjct: 301 YLPNWPERVMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTQGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRS+SFWDANVRTI TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTINTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVP+NLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPQNLLWEAVNTHG 447

[Close]

BLAST of Spg023966 vs. NCBI nr
Match: XP_031743128.1 (delta(8)-fatty-acid desaturase 2 [Cucumis sativus] >ANN11751.1 delta8-sphingolipid desaturase [Cucumis sativus] >KGN45699.1 hypothetical protein Csa_005537 [Cucumis sativus])

HSP 1 Score: 889.0 bits (2296), Expect = 1.7e-254
Identity = 420/447 (93.96%), Postives = 439/447 (98.21%), Query Frame = 0


Query: 1   MEAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFI 60
           ME +KKYI+SEELLKHNKPGDLWISIQGKVYNV++WAKEHPGGDTPF+NLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKEHPGGDTPFINLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DFQVSEISKDYR+LASEFSKQGLFEKKGHVTMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 121 LMLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSG 180
           +M+FLVVYGV+KSESVLVHL  AM+LGMLWIQSAYVGHDSGHSNVM++RGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FFR+LTSHFYGRKL+FDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFRTLTSHFYGRKLEFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVF+TWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSS 360
           YLPNWPER MFVLASFAV SLQHIQFCLNHFSANVYVGKPTGNDWFEKQ SGTLDI CS+
Sbjct: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQASGTLDISCST 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRS+SFWDANVRTIRTL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Spg023966 vs. NCBI nr
Match: XP_023548521.1 (delta(8)-fatty-acid desaturase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 887.9 bits (2293), Expect = 3.7e-254
Identity = 419/447 (93.74%), Postives = 436/447 (97.54%), Query Frame = 0


Query: 1   MEAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFI 60
           MEA+KKYI+SEELLKHNKPGDLWISIQGK+YNV+DWAKEHPGGDTPFLNL GHDVTDAFI
Sbjct: 1   MEAEKKYITSEELLKHNKPGDLWISIQGKIYNVTDWAKEHPGGDTPFLNLTGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DF VSE+SKDYRRLASEFS+QGLFEKKGHVTMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLEDFHVSEVSKDYRRLASEFSRQGLFEKKGHVTMYSLISVA 120

Query: 121 LMLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSG 180
           +MLFLVVYGVVKSESVLVHL SAM+LGMLWIQSAYVGHDSGHS VM++RGFNKLAQILSG
Sbjct: 121 IMLFLVVYGVVKSESVLVHLGSAMILGMLWIQSAYVGHDSGHSTVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FF+SLTSHFYGR LKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFKSLTSHFYGRTLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVS 300
           LARFF+SYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVF+TWFPLLVS
Sbjct: 241 LARFFISYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSS 360
           YLPNWPER MFVLASFAVTSLQHIQFCLNHFSANVYVGKP GNDWFEKQT GTLDI CSS
Sbjct: 301 YLPNWPERVMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTKGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRS+SFWDANVRTI TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTINTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVP+NLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPQNLLWEAVNTHG 447

[Close]

BLAST of Spg023966 vs. ExPASy Swiss-Prot
Match: Q3EBF7 (Delta(8)-fatty-acid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=SLD2 PE=1 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 2.3e-211
Identity = 334/446 (74.89%), Postives = 389/446 (87.22%), Query Frame = 0


Query: 2   EAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFIA 61
           + KK+Y++SE+L KHNKPGDLWISIQGKVY+VSDW K HPGG+   LNLAG DVTDAFIA
Sbjct: 4   QTKKRYVTSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAFIA 63

Query: 62  YHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVAL 121
           YHPGTAW +L++L  G+H++D  VS++S+DYRRLA+EFSK+GLF+KKGHVT+Y+L  V +
Sbjct: 64  YHPGTAWHHLEKLHNGYHVRDHHVSDVSRDYRRLAAEFSKRGLFDKKGHVTLYTLTCVGV 123

Query: 122 MLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSGN 181
           ML  V+YGV+   S+  HL SA+LLG+LWIQSAYVGHDSGH  V +T+  NKL Q+LSGN
Sbjct: 124 MLAAVLYGVLACTSIWAHLISAVLLGLLWIQSAYVGHDSGHYTVTSTKPCNKLIQLLSGN 183

Query: 182 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDSL 241
           CLTGISIAWWKWTHNAHHI+CNSLDHDPDLQHIP+FAVS  FF S+TS FYGRKL FD L
Sbjct: 184 CLTGISIAWWKWTHNAHHIACNSLDHDPDLQHIPIFAVSTKFFNSMTSRFYGRKLTFDPL 243

Query: 242 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVSY 301
           ARF +SYQH+TFYPVMCV R+NLF+QT LLLFS R VPDRALNI GILVF+TWFPLLVS+
Sbjct: 244 ARFLISYQHWTFYPVMCVGRINLFIQTFLLLFSKRHVPDRALNIAGILVFWTWFPLLVSF 303

Query: 302 LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSSW 361
           LPNW ERF+FV  SFAVT++QH+QFCLNHF+A+VY G P GNDWFEKQT+GTLDI C S+
Sbjct: 304 LPNWQERFIFVFVSFAVTAIQHVQFCLNHFAADVYTGPPNGNDWFEKQTAGTLDISCRSF 363

Query: 362 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTLR 421
           MDWFFGGLQFQLEHHLFPR+PRC LR +SP+V ELCKKHNLPYRS+S+W+ANV TIRTL+
Sbjct: 364 MDWFFGGLQFQLEHHLFPRLPRCHLRTVSPVVKELCKKHNLPYRSLSWWEANVWTIRTLK 423

Query: 422 TAALQARDLTNPVPKNLLWEAVNTHG 448
            AA+QARD TNPV KNLLWEAVNTHG
Sbjct: 424 NAAIQARDATNPVLKNLLWEAVNTHG 449

[Close]

BLAST of Spg023966 vs. ExPASy Swiss-Prot
Match: Q9ZRP7 (Delta(8)-fatty-acid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=SLD1 PE=1 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 2.3e-206
Identity = 329/446 (73.77%), Postives = 382/446 (85.65%), Query Frame = 0


Query: 2   EAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFIA 61
           E +KKYI++E+L KHNK GDLWI+IQGKVYNVSDW K HPGGDT  LNL G DVTDAFIA
Sbjct: 4   ETEKKYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAFIA 63

Query: 62  YHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVAL 121
           +HPGTAW +LD LFTG+H++DFQVSE+S+DYRR+A+EF K GLFE KGHVT+Y+L  VA 
Sbjct: 64  FHPGTAWHHLDHLFTGYHIRDFQVSEVSRDYRRMAAEFRKLGLFENKGHVTLYTLAFVAA 123

Query: 122 MLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSGN 181
           M   V+YGV+   SV  H  +A LLG+LWIQSAY+GHDSGH  +M+ + +N+ AQ+LSGN
Sbjct: 124 MFLGVLYGVLACTSVFAHQIAAALLGLLWIQSAYIGHDSGHYVIMSNKSYNRFAQLLSGN 183

Query: 182 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDSL 241
           CLTGISIAWWKWTHNAHH++CNSLD+DPDLQHIPVFAVS  FF SLTS FY RKL FD +
Sbjct: 184 CLTGISIAWWKWTHNAHHLACNSLDYDPDLQHIPVFAVSTKFFSSLTSRFYDRKLTFDPV 243

Query: 242 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVSY 301
           ARF VSYQHFT+YPVMC  R+NLF+QT LLLFS R+VPDRALN  GILVF+TWFPLLVS 
Sbjct: 244 ARFLVSYQHFTYYPVMCFGRINLFIQTFLLLFSKREVPDRALNFAGILVFWTWFPLLVSC 303

Query: 302 LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSSW 361
           LPNWPERF FV  SF VT+LQHIQF LNHF+A+VYVG PTG+DWFEKQ +GT+DI C S+
Sbjct: 304 LPNWPERFFFVFTSFTVTALQHIQFTLNHFAADVYVGPPTGSDWFEKQAAGTIDISCRSY 363

Query: 362 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTLR 421
           MDWFFGGLQFQLEHHLFPR+PRC LRK+SP+V ELCKKHNLPYRSMS+++ANV TI TL+
Sbjct: 364 MDWFFGGLQFQLEHHLFPRLPRCHLRKVSPVVQELCKKHNLPYRSMSWFEANVLTINTLK 423

Query: 422 TAALQARDLTNPVPKNLLWEAVNTHG 448
           TAA QARD+ NPV KNL+WEA+NTHG
Sbjct: 424 TAAYQARDVANPVVKNLVWEALNTHG 449

[Close]

BLAST of Spg023966 vs. ExPASy Swiss-Prot
Match: Q9FR82 (Delta(8)-fatty-acid desaturase OS=Borago officinalis OX=13363 GN=sld1 PE=1 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 1.5e-202
Identity = 331/447 (74.05%), Postives = 384/447 (85.91%), Query Frame = 0


Query: 1   MEAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFI 60
           ME  KKYIS  EL KHN+ GD+WISIQGKVYNV+DW K+HPGGD P +NLAG D TDAFI
Sbjct: 1   MEGTKKYISVGELEKHNQLGDVWISIQGKVYNVTDWIKKHPGGDVPIMNLAGQDATDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAW  L+ LFTG+HL+D+ VSEISKDYR+LASEFSK GLFEKKGH  +Y L  +A
Sbjct: 61  AYHPGTAWKNLENLFTGYHLEDYLVSEISKDYRKLASEFSKAGLFEKKGHTVIYCLSFIA 120

Query: 121 LMLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSG 180
           L+L   VYGV+ S S+ VH+ S  +LGM +IQ+AY+GHDSGH  +M+++G+NK AQ+L+G
Sbjct: 121 LLLCGCVYGVLCSNSLWVHMLSGAMLGMCFIQAAYLGHDSGHYTMMSSKGYNKFAQVLNG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHI+CNSLD+DPDLQH+PVFAV ++FF+SLTS FYGR+L FD 
Sbjct: 181 NCLTGISIAWWKWTHNAHHIACNSLDYDPDLQHLPVFAVPSSFFKSLTSRFYGRELTFDG 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVS 300
           L+RF VSYQHFT Y VM   R+NL+VQT LLLFSTRKVPDRALNI+GILV++TWFP LVS
Sbjct: 241 LSRFLVSYQHFTIYLVMIFGRINLYVQTFLLLFSTRKVPDRALNIIGILVYWTWFPYLVS 300

Query: 301 YLPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSS 360
            LPNW ER +FVL  F+VT+LQHIQF LNHF+A+VYVG PTG +WFEKQ +GT+DI CSS
Sbjct: 301 CLPNWNERVLFVLTCFSVTALQHIQFTLNHFAADVYVGPPTGTNWFEKQAAGTIDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLR ISPIV + CKKHNLPYRS+SF+DANV TI+TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRNISPIVQDYCKKHNLPYRSLSFFDANVATIKTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLT  VP+NLLWEA NTHG
Sbjct: 421 RTAALQARDLT-VVPQNLLWEAFNTHG 446

[Close]

BLAST of Spg023966 vs. ExPASy Swiss-Prot
Match: Q43469 (Delta(8)-fatty-acid desaturase OS=Helianthus annuus OX=4232 GN=sld1 PE=1 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 1.7e-201
Identity = 319/443 (72.01%), Postives = 378/443 (85.33%), Query Frame = 0


Query: 5   KKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFIAYHP 64
           KKYI+S+EL KHN P DLWISI GKVYNV++WAKEHPGGD P +NLAG DVTDAFIA+HP
Sbjct: 16  KKYITSKELKKHNNPNDLWISILGKVYNVTEWAKEHPGGDAPLINLAGQDVTDAFIAFHP 75

Query: 65  GTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVALMLF 124
           GTAW +LD+LFTG+HL+D+QVS+IS+DYR+LASEF+K G+FEKKGH  +YSL  V+L+L 
Sbjct: 76  GTAWKHLDKLFTGYHLKDYQVSDISRDYRKLASEFAKAGMFEKKGHGVIYSLCFVSLLLS 135

Query: 125 LVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSGNCLT 184
             VYGV+ S S  +H+ S  +LG+ W+Q AY+GHD+GH  +M TRG+NK A I  GNC+T
Sbjct: 136 ACVYGVLYSGSFWIHMLSGAILGLAWMQIAYLGHDAGHYQMMATRGWNKFAGIFIGNCIT 195

Query: 185 GISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDSLARF 244
           GISIAWWKWTHNAHHI+CNSLD+DPDLQH+P+ AVS+  F S+TS FYGR+L FD LARF
Sbjct: 196 GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNSITSVFYGRQLTFDPLARF 255

Query: 245 FVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVSYLPN 304
           FVSYQH+ +YP+MCVARVNL++QT+LLL S RK+PDR LNI+G L+F+TWFPLLVS LPN
Sbjct: 256 FVSYQHYLYYPIMCVARVNLYLQTILLLISKRKIPDRGLNILGTLIFWTWFPLLVSRLPN 315

Query: 305 WPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSSWMDW 364
           WPER  FVL SF VT +QHIQF LNHFS +VYVG P G++WFEKQT GT+DI CSSWMDW
Sbjct: 316 WPERVAFVLVSFCVTGIQHIQFTLNHFSGDVYVGPPKGDNWFEKQTRGTIDIACSSWMDW 375

Query: 365 FFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTLRTAA 424
           FFGGLQFQLEHHLFPR+PRC LR ISPI  ELCKK+NLPY S+SF+DANV T++TLRTAA
Sbjct: 376 FFGGLQFQLEHHLFPRLPRCHLRSISPICRELCKKYNLPYVSLSFYDANVTTLKTLRTAA 435

Query: 425 LQARDLTNPVPKNLLWEAVNTHG 448
           LQARDLTNP P+NL WEA NTHG
Sbjct: 436 LQARDLTNPAPQNLAWEAFNTHG 458

[Close]

BLAST of Spg023966 vs. ExPASy Swiss-Prot
Match: O04353 (Acyl-lipid (9-3)-desaturase OS=Borago officinalis OX=13363 PE=1 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 8.1e-180
Identity = 283/443 (63.88%), Postives = 353/443 (79.68%), Query Frame = 0


Query: 5   KKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFIAYHP 64
           KKYI+S+EL  H+KPGDLWISIQGK Y+VSDW K+HPGG  P  +LAG +VTDAF+A+HP
Sbjct: 6   KKYITSDELKNHDKPGDLWISIQGKAYDVSDWVKDHPGGSFPLKSLAGQEVTDAFVAFHP 65

Query: 65  GTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVALMLF 124
            + W  LD+ FTG++L+D+ VSE+SKDYR+L  EFSK GL++KKGH+   +L  +A++  
Sbjct: 66  ASTWKNLDKFFTGYYLKDYSVSEVSKDYRKLVFEFSKMGLYDKKGHIMFATLCFIAMLFA 125

Query: 125 LVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSGNCLT 184
           + VYGV+  E VLVHL S  L+G LWIQS ++GHD+GH  V++    NK   I + NCL+
Sbjct: 126 MSVYGVLFCEGVLVHLFSGCLMGFLWIQSGWIGHDAGHYMVVSDSRLNKFMGIFAANCLS 185

Query: 185 GISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDSLARF 244
           GISI WWKW HNAHHI+CNSL++DPDLQ+IP   VS+ FF SLTSHFY ++L FDSL+RF
Sbjct: 186 GISIGWWKWNHNAHHIACNSLEYDPDLQYIPFLVVSSKFFGSLTSHFYEKRLTFDSLSRF 245

Query: 245 FVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVSYLPN 304
           FVSYQH+TFYP+MC AR+N++VQ+L++L + R V  RA  ++G LVF  W+PLLVS LPN
Sbjct: 246 FVSYQHWTFYPIMCAARLNMYVQSLIMLLTKRNVSYRAHELLGCLVFSIWYPLLVSCLPN 305

Query: 305 WPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSSWMDW 364
           W ER MFV+AS +VT +Q +QF LNHFS++VYVGKP GN+WFEKQT GTLDI C  WMDW
Sbjct: 306 WGERIMFVIASLSVTGMQQVQFSLNHFSSSVYVGKPKGNNWFEKQTDGTLDISCPPWMDW 365

Query: 365 FFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTLRTAA 424
           F GGLQFQ+EHHLFP+MPRC LRKISP V+ELCKKHNLPY   SF  AN  T+RTLR  A
Sbjct: 366 FHGGLQFQIEHHLFPKMPRCNLRKISPYVIELCKKHNLPYNYASFSKANEMTLRTLRNTA 425

Query: 425 LQARDLTNPVPKNLLWEAVNTHG 448
           LQARD+T P+PKNL+WEA++THG
Sbjct: 426 LQARDITKPLPKNLVWEALHTHG 448

[Close]

BLAST of Spg023966 vs. ExPASy TrEMBL
Match: A0A6J1KW79 (delta(8)-fatty-acid desaturase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111499262 PE=4 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 2.1e-255
Identity = 422/447 (94.41%), Postives = 437/447 (97.76%), Query Frame = 0


Query: 1   MEAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFI 60
           MEA+KKYI+SEELLKHNKPGDLWISIQGKVYNV+DWAKEHPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEAEKKYITSEELLKHNKPGDLWISIQGKVYNVTDWAKEHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DF VSE+SKDYRRLASEFSKQGLFEKKGHVTMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLEDFHVSEVSKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 121 LMLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSG 180
           +MLFLVVYGVVKSESVLVHL SAM+LGMLWIQSAYVGHDSGHS VM++RGFNKLAQILSG
Sbjct: 121 IMLFLVVYGVVKSESVLVHLGSAMILGMLWIQSAYVGHDSGHSTVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FF+SLTSHFYGR LKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFKSLTSHFYGRTLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVS 300
           LARFF+SYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVF+TWFPLLVS
Sbjct: 241 LARFFISYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSS 360
           YLPNWPER MFVLASFAVTSLQHIQFCLNHFSANVYVGKP GNDWFEKQT GTLDI CSS
Sbjct: 301 YLPNWPERVMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTKGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRS+SFWDANVRTI TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTINTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVP+NLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPQNLLWEAVNTHG 447

[Close]

BLAST of Spg023966 vs. ExPASy TrEMBL
Match: A0A5D3CS55 (Delta(8)-fatty-acid desaturase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold268G00250 PE=4 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 2.8e-255
Identity = 423/447 (94.63%), Postives = 438/447 (97.99%), Query Frame = 0


Query: 1   MEAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFI 60
           ME +KKYI+SEELLKHNKPGDLWISIQGKVYNV++WAK HPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DFQVSEISKDYR+LASEFSKQGLFEKKGHV MYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120

Query: 121 LMLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSG 180
           +M+FLVVYGV+KSESVLVHL  AM+LGMLWIQSAYVGHDSGHSNVM++RGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSA FFR+LTSHFYGRKLKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVF+TWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSS 360
           YLPNWPER MFVLASFAV SLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDI CSS
Sbjct: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRS+SFWDANVRTIRTL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Spg023966 vs. ExPASy TrEMBL
Match: A0A1S3C7C0 (delta(8)-fatty-acid desaturase 2 OS=Cucumis melo OX=3656 GN=LOC103497683 PE=4 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 2.8e-255
Identity = 423/447 (94.63%), Postives = 438/447 (97.99%), Query Frame = 0


Query: 1   MEAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFI 60
           ME +KKYI+SEELLKHNKPGDLWISIQGKVYNV++WAK HPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DFQVSEISKDYR+LASEFSKQGLFEKKGHV MYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120

Query: 121 LMLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSG 180
           +M+FLVVYGV+KSESVLVHL  AM+LGMLWIQSAYVGHDSGHSNVM++RGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSA FFR+LTSHFYGRKLKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVF+TWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSS 360
           YLPNWPER MFVLASFAV SLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDI CSS
Sbjct: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRS+SFWDANVRTIRTL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Spg023966 vs. ExPASy TrEMBL
Match: A0A6J1H485 (delta(8)-fatty-acid desaturase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460269 PE=4 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 4.7e-255
Identity = 421/447 (94.18%), Postives = 437/447 (97.76%), Query Frame = 0


Query: 1   MEAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFI 60
           MEA+KKYI+SEELLKHNKPGDLWISIQGKVYNV+DWAKEHPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEAEKKYITSEELLKHNKPGDLWISIQGKVYNVTDWAKEHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DF VSE+SKDYRRLASEFS+QGLFEKKGHVTMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLEDFHVSEVSKDYRRLASEFSRQGLFEKKGHVTMYSLISVA 120

Query: 121 LMLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSG 180
           +MLFLVVYGVVKSESVLVHL SAM+LGMLWIQSAYVGHDSGHS VM++RGFNKLAQILSG
Sbjct: 121 IMLFLVVYGVVKSESVLVHLGSAMILGMLWIQSAYVGHDSGHSTVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FF+SLTSHFYGR LKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFKSLTSHFYGRTLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVS 300
           LARFF+SYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVF+TWFPLLVS
Sbjct: 241 LARFFISYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSS 360
           YLPNWPER MFVLASFAVTSLQHIQFCLNHFSANVYVGKP GNDWFEKQT GTLDI CSS
Sbjct: 301 YLPNWPERVMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTQGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRS+SFWDANVRTI TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTINTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVP+NLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPQNLLWEAVNTHG 447

[Close]

BLAST of Spg023966 vs. ExPASy TrEMBL
Match: A0A0A0KA29 (Delta8-sphingolipid desaturase OS=Cucumis sativus OX=3659 GN=Csa_6G006850 PE=4 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 8.0e-255
Identity = 420/447 (93.96%), Postives = 439/447 (98.21%), Query Frame = 0


Query: 1   MEAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFI 60
           ME +KKYI+SEELLKHNKPGDLWISIQGKVYNV++WAKEHPGGDTPF+NLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKEHPGGDTPFINLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DFQVSEISKDYR+LASEFSKQGLFEKKGHVTMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 121 LMLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSG 180
           +M+FLVVYGV+KSESVLVHL  AM+LGMLWIQSAYVGHDSGHSNVM++RGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FFR+LTSHFYGRKL+FDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFRTLTSHFYGRKLEFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVF+TWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSS 360
           YLPNWPER MFVLASFAV SLQHIQFCLNHFSANVYVGKPTGNDWFEKQ SGTLDI CS+
Sbjct: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQASGTLDISCST 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRS+SFWDANVRTIRTL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Spg023966 vs. TAIR 10
Match: AT2G46210.1 (Fatty acid/sphingolipid desaturase )

HSP 1 Score: 736.1 bits (1899), Expect = 1.7e-212
Identity = 334/446 (74.89%), Postives = 389/446 (87.22%), Query Frame = 0


Query: 2   EAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFIA 61
           + KK+Y++SE+L KHNKPGDLWISIQGKVY+VSDW K HPGG+   LNLAG DVTDAFIA
Sbjct: 4   QTKKRYVTSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAFIA 63

Query: 62  YHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVAL 121
           YHPGTAW +L++L  G+H++D  VS++S+DYRRLA+EFSK+GLF+KKGHVT+Y+L  V +
Sbjct: 64  YHPGTAWHHLEKLHNGYHVRDHHVSDVSRDYRRLAAEFSKRGLFDKKGHVTLYTLTCVGV 123

Query: 122 MLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSGN 181
           ML  V+YGV+   S+  HL SA+LLG+LWIQSAYVGHDSGH  V +T+  NKL Q+LSGN
Sbjct: 124 MLAAVLYGVLACTSIWAHLISAVLLGLLWIQSAYVGHDSGHYTVTSTKPCNKLIQLLSGN 183

Query: 182 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDSL 241
           CLTGISIAWWKWTHNAHHI+CNSLDHDPDLQHIP+FAVS  FF S+TS FYGRKL FD L
Sbjct: 184 CLTGISIAWWKWTHNAHHIACNSLDHDPDLQHIPIFAVSTKFFNSMTSRFYGRKLTFDPL 243

Query: 242 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVSY 301
           ARF +SYQH+TFYPVMCV R+NLF+QT LLLFS R VPDRALNI GILVF+TWFPLLVS+
Sbjct: 244 ARFLISYQHWTFYPVMCVGRINLFIQTFLLLFSKRHVPDRALNIAGILVFWTWFPLLVSF 303

Query: 302 LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSSW 361
           LPNW ERF+FV  SFAVT++QH+QFCLNHF+A+VY G P GNDWFEKQT+GTLDI C S+
Sbjct: 304 LPNWQERFIFVFVSFAVTAIQHVQFCLNHFAADVYTGPPNGNDWFEKQTAGTLDISCRSF 363

Query: 362 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTLR 421
           MDWFFGGLQFQLEHHLFPR+PRC LR +SP+V ELCKKHNLPYRS+S+W+ANV TIRTL+
Sbjct: 364 MDWFFGGLQFQLEHHLFPRLPRCHLRTVSPVVKELCKKHNLPYRSLSWWEANVWTIRTLK 423

Query: 422 TAALQARDLTNPVPKNLLWEAVNTHG 448
            AA+QARD TNPV KNLLWEAVNTHG
Sbjct: 424 NAAIQARDATNPVLKNLLWEAVNTHG 449

[Close]

BLAST of Spg023966 vs. TAIR 10
Match: AT3G61580.1 (Fatty acid/sphingolipid desaturase )

HSP 1 Score: 719.5 bits (1856), Expect = 1.6e-207
Identity = 329/446 (73.77%), Postives = 382/446 (85.65%), Query Frame = 0


Query: 2   EAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAFIA 61
           E +KKYI++E+L KHNK GDLWI+IQGKVYNVSDW K HPGGDT  LNL G DVTDAFIA
Sbjct: 4   ETEKKYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAFIA 63

Query: 62  YHPGTAWAYLDRLFTGFHLQDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVAL 121
           +HPGTAW +LD LFTG+H++DFQVSE+S+DYRR+A+EF K GLFE KGHVT+Y+L  VA 
Sbjct: 64  FHPGTAWHHLDHLFTGYHIRDFQVSEVSRDYRRMAAEFRKLGLFENKGHVTLYTLAFVAA 123

Query: 122 MLFLVVYGVVKSESVLVHLASAMLLGMLWIQSAYVGHDSGHSNVMTTRGFNKLAQILSGN 181
           M   V+YGV+   SV  H  +A LLG+LWIQSAY+GHDSGH  +M+ + +N+ AQ+LSGN
Sbjct: 124 MFLGVLYGVLACTSVFAHQIAAALLGLLWIQSAYIGHDSGHYVIMSNKSYNRFAQLLSGN 183

Query: 182 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSANFFRSLTSHFYGRKLKFDSL 241
           CLTGISIAWWKWTHNAHH++CNSLD+DPDLQHIPVFAVS  FF SLTS FY RKL FD +
Sbjct: 184 CLTGISIAWWKWTHNAHHLACNSLDYDPDLQHIPVFAVSTKFFSSLTSRFYDRKLTFDPV 243

Query: 242 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFYTWFPLLVSY 301
           ARF VSYQHFT+YPVMC  R+NLF+QT LLLFS R+VPDRALN  GILVF+TWFPLLVS 
Sbjct: 244 ARFLVSYQHFTYYPVMCFGRINLFIQTFLLLFSKREVPDRALNFAGILVFWTWFPLLVSC 303

Query: 302 LPNWPERFMFVLASFAVTSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDIDCSSW 361
           LPNWPERF FV  SF VT+LQHIQF LNHF+A+VYVG PTG+DWFEKQ +GT+DI C S+
Sbjct: 304 LPNWPERFFFVFTSFTVTALQHIQFTLNHFAADVYVGPPTGSDWFEKQAAGTIDISCRSY 363

Query: 362 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSMSFWDANVRTIRTLR 421
           MDWFFGGLQFQLEHHLFPR+PRC LRK+SP+V ELCKKHNLPYRSMS+++ANV TI TL+
Sbjct: 364 MDWFFGGLQFQLEHHLFPRLPRCHLRKVSPVVQELCKKHNLPYRSMSWFEANVLTINTLK 423

Query: 422 TAALQARDLTNPVPKNLLWEAVNTHG 448
           TAA QARD+ NPV KNL+WEA+NTHG
Sbjct: 424 TAAYQARDVANPVVKNLVWEALNTHG 449

[Close]

BLAST of Spg023966 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 72.4 bits (176), Expect = 1.0e-12
Identity = 48/126 (38.10%), Postives = 67/126 (53.17%), Query Frame = 0


Query: 1   MEAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAF- 60
           M    K  S EE   HNK  D W+ I GKVY+VS +  EHPGGD   L +AG D TD F 
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 61  IAYHPGTAWAYLDRLFTGFHLQDFQVSEIS--KDYRRLASEFSKQGLFE--KKGHVTMYS 120
            A H   A   +++ F G  L +  + EI   K Y++   + S Q LF+  K+  V   S
Sbjct: 61  DAGHSKDARELMEKYFIG-ELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVS 120

Query: 121 LISVAL 122
           +I++++
Sbjct: 121 IITISV 125

[Close]

BLAST of Spg023966 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 68.2 bits (165), Expect = 2.0e-11
Identity = 33/78 (42.31%), Postives = 46/78 (58.97%), Query Frame = 0


Query: 1  MEAKKKYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAF- 60
          M + +K +S EE+ KHNK  D W+ I GKVY+V+ +  +HPGGD   L+  G D T+ F 
Sbjct: 1  MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61 IAYHPGTAWAYLDRLFTG 78
             H  TA   +D+ F G
Sbjct: 61 DVGHSDTARDMMDKYFIG 78

[Close]

BLAST of Spg023966 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 60.8 bits (146), Expect = 3.1e-09
Identity = 30/73 (41.10%), Postives = 39/73 (53.42%), Query Frame = 0


Query: 6  KYISSEELLKHNKPGDLWISIQGKVYNVSDWAKEHPGGDTPFLNLAGHDVTDAF-IAYHP 65
          K  +  E+ +H+   D WI I GKVY+V+ +  +HPGGD   L   G D TD F    H 
Sbjct: 6  KVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHS 65

Query: 66 GTAWAYLDRLFTG 78
           TA A LD  + G
Sbjct: 66 STAKAMLDEYYVG 78

The following BLAST results are available for this feature:

BLAST of Spg023966 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023006577.1	4.4e-255	94.41	delta(8)-fatty-acid desaturase 2-like [Cucurbita maxima] >XP_023006578.1 delta(8...	[more]
XP_008458161.1	5.7e-255	94.63	PREDICTED: delta(8)-fatty-acid desaturase 2 [Cucumis melo] >TYK14028.1 delta(8)-...	[more]
XP_022959210.1	9.7e-255	94.18	delta(8)-fatty-acid desaturase 2-like [Cucurbita moschata] >XP_022959211.1 delta...	[more]
XP_031743128.1	1.7e-254	93.96	delta(8)-fatty-acid desaturase 2 [Cucumis sativus] >ANN11751.1 delta8-sphingolip...	[more]
XP_023548521.1	3.7e-254	93.74	delta(8)-fatty-acid desaturase 2-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg023966 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3EBF7	2.3e-211	74.89	Delta(8)-fatty-acid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=SLD2 PE=1 SV...	[more]
Q9ZRP7	2.3e-206	73.77	Delta(8)-fatty-acid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=SLD1 PE=1 SV...	[more]
Q9FR82	1.5e-202	74.05	Delta(8)-fatty-acid desaturase OS=Borago officinalis OX=13363 GN=sld1 PE=1 SV=1	[more]
Q43469	1.7e-201	72.01	Delta(8)-fatty-acid desaturase OS=Helianthus annuus OX=4232 GN=sld1 PE=1 SV=1	[more]
O04353	8.1e-180	63.88	Acyl-lipid (9-3)-desaturase OS=Borago officinalis OX=13363 PE=1 SV=1	[more]

BLAST of Spg023966 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KW79	2.1e-255	94.41	delta(8)-fatty-acid desaturase 2-like OS=Cucurbita maxima OX=3661 GN=LOC11149926...	[more]
A0A5D3CS55	2.8e-255	94.63	Delta(8)-fatty-acid desaturase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C7C0	2.8e-255	94.63	delta(8)-fatty-acid desaturase 2 OS=Cucumis melo OX=3656 GN=LOC103497683 PE=4 SV...	[more]
A0A6J1H485	4.7e-255	94.18	delta(8)-fatty-acid desaturase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460...	[more]
A0A0A0KA29	8.0e-255	93.96	Delta8-sphingolipid desaturase OS=Cucumis sativus OX=3659 GN=Csa_6G006850 PE=4 S...	[more]

BLAST of Spg023966 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G46210.1	1.7e-212	74.89	Fatty acid/sphingolipid desaturase	[more]
AT3G61580.1	1.6e-207	73.77	Fatty acid/sphingolipid desaturase	[more]
AT1G26340.1	1.0e-12	38.10	cytochrome B5 isoform A	[more]
AT5G53560.1	2.0e-11	42.31	cytochrome B5 isoform E	[more]
AT5G48810.1	3.1e-09	41.10	cytochrome B5 isoform D	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..80 e-value: 1.8E-19 score: 80.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 10..77 e-value: 3.0E-21 score: 75.3
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..89 score: 22.267321
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 139..406 e-value: 7.2E-36 score: 124.3
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 1..79 e-value: 6.4E-21 score: 76.3
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 4..85
IPR012171	Fatty acid desaturase	PIRSF	PIRSF015921	FA_sphingolip_des	coord: 1..434 e-value: 1.3E-128 score: 427.7
None	No IPR available	PANTHER	PTHR19353	FATTY ACID DESATURASE 2	coord: 1..446
None	No IPR available	PANTHER	PTHR19353:SF28	DELTA(8)-FATTY-ACID DESATURASE 2	coord: 1..446
None	No IPR available	CDD	cd03506	Delta6-FADS-like	coord: 140..402 e-value: 3.36006E-63 score: 201.331

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg023966.1	Spg023966.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
molecular_function	GO:0016491	oxidoreductase activity