Spg023957 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg023957
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAnkyrin repeat-containing protein
Locationscaffold13: 1174097 .. 1175676 (-)
RNA-Seq ExpressionSpg023957
SyntenySpg023957
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGAGAATCGCCGAGATCCCACGACCTCTTTCAATTCTCCGGATGCACCAAATGAAGAAATTACAATTGCGATGTGTTCTGTCGAAGAAAAAATAACAAAGCTCTATGAGGCATCAAAGAGTGGATCTATCCAAACTTTGAAAACACTCATTCAAGAGGATCCAAATCTCATTCAAAAAACTCTAACCTCCACTTCCAACGTTGAGAGCCCATTGCACGTTGCAGTTTCCCATGGCCATCTTGAATTCACTCGTTTGCTTCTAGATCACAACCCTGAACTTGCCGCTGAGGTTGACGCCTTTCAACGAACACCCCTCCACCTGGCTTCTAAACATGGAGACATGGAGACCATTCAAGCTTTGTTGGAGAAGAACACCAGTGCTTGCTTGGTTTACGATAAGAATGGGTTGATTCCTCTCCACTATGCAGTAATTGGTGGACAAATCGAGATCATGCTAGTGCTTATCAACGCAAGACCACAATCTTTTTGGATGAAGCTCAACAATGGTCAAACTATTCTACATTTATGTGTAGAAAATAACCATTTGGAGGGGATAAAGTTGCTTGTTGAAACATTTATGAATCGTGATGAAAACTTCCTAAACACAATTGATGACAAGGGAAACACTGTTTTGGATCTCTCATTGATGCTGAGACAAAGCGAGGTAAGTAATAATTAGAGAAACCCGATAAAAGAGTTCCCTTGATTATCGTAATCAACCAGAGATATATCGTGTTTAATTATATGTGGATATGTGATCAAAACCTTTTCCAACTTCTTTTCTTTTTTTATTCTTGTTAGATGGTGGGGTATTTAATCTCGATTCCAGAAGTAAAAACTAGAACAAGCAGTAGAAAGGACTTTGCAGCCTCAAATGGCACAAAAGAAAGTTCAGAACCTCAAGAGTCAAGGGGGCGTTGGAAGGTAATGAGGAAGAATTTGAAGTACAAAGGGGATTGGGTACAAGAAGTACAAGGGACAATGATGTTAGTGGCTACAGTGATAGCAACCGTGACTTTTCAAGCTGGAATCAACCCTCCCGGCGGTGTTTGGCAACAAGATACTCCATTCAATTGTTCGAGCCACATAGAATGGATCTCACGTTACAACCTTCCTTGCTTCGGCATCAATGGATCCATCATTTTCCCAGCTGGAACGTCAATAATGAGTGTCCAACGACCCTTTGATTACTGGTCTTACTTGGGGATGAATACGATATCGTTCTTGGCATCGTTGAGCGTGATTTTACTGATCATCAGTCGGTTTCCTCTTAAAAATAGGATTGGCAGTTGGTTGTTGGCCTTTGCCATGTGTGTAGCAGTGGTGTCCTTAGGACTTGGGTATTTTTTCGGAGTTAGATTTATTAGCTCAGTGACCCTAGATTCCTCTGTACCCTCTGATTCCTCCTATTCATCCTTTACATTATTTTCTTTTACACTTCTTTGGTACGTGGTGGTCGGAGTGGTTGTCTTGTGGCACGTAATTCGCTTTCAGGTTTGGATGGTCAAGAGCTTATTCCGCACTTTCACCTCTAAGCTCAAGTCTTACAGCTTCAATCATCGCGCCACACCTTGA

mRNA sequence

ATGGAGGAGAATCGCCGAGATCCCACGACCTCTTTCAATTCTCCGGATGCACCAAATGAAGAAATTACAATTGCGATGTGTTCTGTCGAAGAAAAAATAACAAAGCTCTATGAGGCATCAAAGAGTGGATCTATCCAAACTTTGAAAACACTCATTCAAGAGGATCCAAATCTCATTCAAAAAACTCTAACCTCCACTTCCAACGTTGAGAGCCCATTGCACGTTGCAGTTTCCCATGGCCATCTTGAATTCACTCGTTTGCTTCTAGATCACAACCCTGAACTTGCCGCTGAGGTTGACGCCTTTCAACGAACACCCCTCCACCTGGCTTCTAAACATGGAGACATGGAGACCATTCAAGCTTTGTTGGAGAAGAACACCAGTGCTTGCTTGGTTTACGATAAGAATGGGTTGATTCCTCTCCACTATGCAGTAATTGGTGGACAAATCGAGATCATGCTAGTGCTTATCAACGCAAGACCACAATCTTTTTGGATGAAGCTCAACAATGGTCAAACTATTCTACATTTATGTGTAGAAAATAACCATTTGGAGGGGATAAAGTTGCTTGTTGAAACATTTATGAATCGTGATGAAAACTTCCTAAACACAATTGATGACAAGGGAAACACTGTTTTGGATCTCTCATTGATGCTGAGACAAAGCGAGATGGTGGGGTATTTAATCTCGATTCCAGAAGTAAAAACTAGAACAAGCAGTAGAAAGGACTTTGCAGCCTCAAATGGCACAAAAGAAAGTTCAGAACCTCAAGAGTCAAGGGGGCGTTGGAAGGTAATGAGGAAGAATTTGAAGTACAAAGGGGATTGGGTACAAGAAGTACAAGGGACAATGATGTTAGTGGCTACAGTGATAGCAACCGTGACTTTTCAAGCTGGAATCAACCCTCCCGGCGGTGTTTGGCAACAAGATACTCCATTCAATTGTTCGAGCCACATAGAATGGATCTCACGTTACAACCTTCCTTGCTTCGGCATCAATGGATCCATCATTTTCCCAGCTGGAACGTCAATAATGAGTGTCCAACGACCCTTTGATTACTGGTCTTACTTGGGGATGAATACGATATCGTTCTTGGCATCGTTGAGCGTGATTTTACTGATCATCAGTCGGTTTCCTCTTAAAAATAGGATTGGCAGTTGGTTGTTGGCCTTTGCCATGTGTGTAGCAGTGGTGTCCTTAGGACTTGGGTATTTTTTCGGAGTTAGATTTATTAGCTCAGTGACCCTAGATTCCTCTGTACCCTCTGATTCCTCCTATTCATCCTTTACATTATTTTCTTTTACACTTCTTTGGTACGTGGTGGTCGGAGTGGTTGTCTTGTGGCACGTAATTCGCTTTCAGGTTTGGATGGTCAAGAGCTTATTCCGCACTTTCACCTCTAAGCTCAAGTCTTACAGCTTCAATCATCGCGCCACACCTTGA

Coding sequence (CDS)

ATGGAGGAGAATCGCCGAGATCCCACGACCTCTTTCAATTCTCCGGATGCACCAAATGAAGAAATTACAATTGCGATGTGTTCTGTCGAAGAAAAAATAACAAAGCTCTATGAGGCATCAAAGAGTGGATCTATCCAAACTTTGAAAACACTCATTCAAGAGGATCCAAATCTCATTCAAAAAACTCTAACCTCCACTTCCAACGTTGAGAGCCCATTGCACGTTGCAGTTTCCCATGGCCATCTTGAATTCACTCGTTTGCTTCTAGATCACAACCCTGAACTTGCCGCTGAGGTTGACGCCTTTCAACGAACACCCCTCCACCTGGCTTCTAAACATGGAGACATGGAGACCATTCAAGCTTTGTTGGAGAAGAACACCAGTGCTTGCTTGGTTTACGATAAGAATGGGTTGATTCCTCTCCACTATGCAGTAATTGGTGGACAAATCGAGATCATGCTAGTGCTTATCAACGCAAGACCACAATCTTTTTGGATGAAGCTCAACAATGGTCAAACTATTCTACATTTATGTGTAGAAAATAACCATTTGGAGGGGATAAAGTTGCTTGTTGAAACATTTATGAATCGTGATGAAAACTTCCTAAACACAATTGATGACAAGGGAAACACTGTTTTGGATCTCTCATTGATGCTGAGACAAAGCGAGATGGTGGGGTATTTAATCTCGATTCCAGAAGTAAAAACTAGAACAAGCAGTAGAAAGGACTTTGCAGCCTCAAATGGCACAAAAGAAAGTTCAGAACCTCAAGAGTCAAGGGGGCGTTGGAAGGTAATGAGGAAGAATTTGAAGTACAAAGGGGATTGGGTACAAGAAGTACAAGGGACAATGATGTTAGTGGCTACAGTGATAGCAACCGTGACTTTTCAAGCTGGAATCAACCCTCCCGGCGGTGTTTGGCAACAAGATACTCCATTCAATTGTTCGAGCCACATAGAATGGATCTCACGTTACAACCTTCCTTGCTTCGGCATCAATGGATCCATCATTTTCCCAGCTGGAACGTCAATAATGAGTGTCCAACGACCCTTTGATTACTGGTCTTACTTGGGGATGAATACGATATCGTTCTTGGCATCGTTGAGCGTGATTTTACTGATCATCAGTCGGTTTCCTCTTAAAAATAGGATTGGCAGTTGGTTGTTGGCCTTTGCCATGTGTGTAGCAGTGGTGTCCTTAGGACTTGGGTATTTTTTCGGAGTTAGATTTATTAGCTCAGTGACCCTAGATTCCTCTGTACCCTCTGATTCCTCCTATTCATCCTTTACATTATTTTCTTTTACACTTCTTTGGTACGTGGTGGTCGGAGTGGTTGTCTTGTGGCACGTAATTCGCTTTCAGGTTTGGATGGTCAAGAGCTTATTCCGCACTTTCACCTCTAAGCTCAAGTCTTACAGCTTCAATCATCGCGCCACACCTTGA

Protein sequence

MEENRRDPTTSFNSPDAPNEEITIAMCSVEEKITKLYEASKSGSIQTLKTLIQEDPNLIQKTLTSTSNVESPLHVAVSHGHLEFTRLLLDHNPELAAEVDAFQRTPLHLASKHGDMETIQALLEKNTSACLVYDKNGLIPLHYAVIGGQIEIMLVLINARPQSFWMKLNNGQTILHLCVENNHLEGIKLLVETFMNRDENFLNTIDDKGNTVLDLSLMLRQSEMVGYLISIPEVKTRTSSRKDFAASNGTKESSEPQESRGRWKVMRKNLKYKGDWVQEVQGTMMLVATVIATVTFQAGINPPGGVWQQDTPFNCSSHIEWISRYNLPCFGINGSIIFPAGTSIMSVQRPFDYWSYLGMNTISFLASLSVILLIISRFPLKNRIGSWLLAFAMCVAVVSLGLGYFFGVRFISSVTLDSSVPSDSSYSSFTLFSFTLLWYVVVGVVVLWHVIRFQVWMVKSLFRTFTSKLKSYSFNHRATP
Homology
BLAST of Spg023957 vs. NCBI nr
Match: XP_008447612.1 (PREDICTED: uncharacterized protein LOC103490026 [Cucumis melo])

HSP 1 Score: 527.7 bits (1358), Expect = 1.0e-145
Identity = 296/513 (57.70%), Postives = 368/513 (71.73%), Query Frame = 0

Query: 1   MEENRRDPTTSFNSPDAPNEEITIAMCSVEEK-ITKLYEASKSGSIQTLKTLIQEDPNLI 60
           MEEN ++ T+ FNS DA N ++ I M S+EE  I KLYEASK G +QTLKTLIQ++P+LI
Sbjct: 1   MEENHQEITSLFNSSDAQNSQVIIEMSSMEEDIIRKLYEASKIGCVQTLKTLIQDNPDLI 60

Query: 61  QKTL--TSTSNVESP-LHVAVSHGHLEFTRLLLDHNPELAAEVDAFQRTPLHLA-SKHGD 120
            K L  TS+  +E+P LHV+V HGHLEFT+LLLDHNP+LAAEVDAFQRTPLH+A S +GD
Sbjct: 61  HKALIYTSSITIETPLLHVSVFHGHLEFTQLLLDHNPQLAAEVDAFQRTPLHIACSNNGD 120

Query: 121 METIQALLEKNTSACLVYDKNGLIPLHYAVIGGQIEIMLVLINARPQSFWMKLNNG--QT 180
           ME I+ALLEKNTSACLV D +GLIPLHYAVI   IE+M +LI ARPQS  MKLNN   +T
Sbjct: 121 MEIIRALLEKNTSACLVQDLSGLIPLHYAVISENIEMMELLIKARPQSVLMKLNNNNDKT 180

Query: 181 ILHLCVENNHLEGIKLLVETFMNRDENFLNTIDDKGNTVLDLSLMLRQSEMVGYLISIPE 240
           +LHLCVE NHLEG+KLL+   +  D++FLN +DD+GNT+LDLSLMLR+ EMVGYL+ IPE
Sbjct: 181 VLHLCVEGNHLEGMKLLIAQTLLFDKDFLNAMDDEGNTILDLSLMLRRIEMVGYLLMIPE 240

Query: 241 VKTRTSSRKD--FAASNGTKESSEPQESR------------GRWKVMRKNLKYKGDWVQE 300
            KTRT+  K+    +   TK  +   + R            GRWKV RK LKY+GDWVQE
Sbjct: 241 AKTRTNDIKEKLLESQKITKTRNRKTQRRESVSLSTKKRPIGRWKVWRKKLKYRGDWVQE 300

Query: 301 VQGTMMLVATVIATVTFQAGINPPGGVWQQDTPFNCSSHI--------EWISRYNL---- 360
           VQGTMMLVATVIATVTFQ G+NPPGGVWQQDTPF  SS I        EW   + L    
Sbjct: 301 VQGTMMLVATVIATVTFQGGVNPPGGVWQQDTPFIYSSIINTTKNGFSEWYKDFGLYEEY 360

Query: 361 PCFGINGSIIFPAGTSIMSVQRPFDYWSYLGMNTISFLASLSVILLIISRFPLKNRIGSW 420
                N S++FPAGT +M  Q+P     YL +NT+SFLAS+SVIL+I+SRFPLKNRI SW
Sbjct: 361 SYLRNNTSVLFPAGTGVMRFQQPLLCSLYLWVNTVSFLASMSVILMIVSRFPLKNRICSW 420

Query: 421 LLAFAMCVAVVSLGLGYFFGVRFISSVTLDSSVPSDSSYSSFTLFSFTLL-WYVVVGVVV 480
           LL   MC+AVVSL +GY  GV+ ++ +T     P    +S+  +FS T++ W+ +VG+V 
Sbjct: 421 LLTLDMCIAVVSLAIGYLLGVKMVNLLTF----PEKEDFSNSDVFSLTVICWFGIVGLVC 480

BLAST of Spg023957 vs. NCBI nr
Match: XP_023526885.1 (ankyrin repeat-containing protein NPR4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.8 bits (1301), Expect = 4.2e-139
Identity = 282/479 (58.87%), Postives = 352/479 (73.49%), Query Frame = 0

Query: 1   MEENRRDPTTSFNS-PDAP-NEEITIAMCSVEEKITKLYEASKSGSIQTLKTLIQEDPNL 60
           ME+N + PT SFN  PDA  N+EI I + SVEE   K+YEASK G IQTLKTLIQEDPN+
Sbjct: 1   MEKNLKHPTPSFNGVPDATRNQEIRIVIGSVEESRRKVYEASKKGCIQTLKTLIQEDPNM 60

Query: 61  IQKTLTSTSNVESP-LHVAVSHGHLEFTRLLLDHNPEL----AAEVDAFQRTPLHLASKH 120
           +   + S+SN  +P LH ++ HGHL+FT+LL  HNPEL     +EVDAFQ TPLHLASK+
Sbjct: 61  VLAVILSSSNNHNPLLHESICHGHLQFTQLLY-HNPELLIVMQSEVDAFQPTPLHLASKN 120

Query: 121 GDMETIQALLEKNTSACLVYDKNGLIPLHYAVIGGQIEIMLVLINARPQSFWMKLNNGQT 180
           GD++T++ALLE NTSACLVYD +G IPLHYAVI GQ++IM  LINA PQS WMKLN+G+T
Sbjct: 121 GDIDTVRALLENNTSACLVYDNDGFIPLHYAVINGQVDIMKELINATPQSIWMKLNDGRT 180

Query: 181 ILHLCVENNHLEGIKLLVETFMNRDENFLNTIDDKGNTVLDLSLMLRQSEMVGYLISIPE 240
           +LHLCVE++HLEGIKLL+ETFMNR E FLNT DDKGNT+LDLS+ LRQSEMVGYL+S+ E
Sbjct: 181 VLHLCVESDHLEGIKLLIETFMNRHEGFLNTTDDKGNTILDLSIKLRQSEMVGYLLSLSE 240

Query: 241 VKTRTSSRKDFAASNGTKESSEPQESR-----------GRWKVMRKNLKYKGDWVQEVQG 300
           VKTR S+     ASN  K S E + S+           GRWK  RK LKYKGDWV+EVQG
Sbjct: 241 VKTRIST-----ASNVRKRSLESRNSKKQRRECASFRIGRWKAWRKKLKYKGDWVEEVQG 300

Query: 301 TMMLVATVIATVTFQAGINPPGGVWQQDTPFNCSSHIEWISRYNLPCFGINGSIIFPAGT 360
           TMMLVATVIATVTFQAG+NP GGVWQQDT +N +++I+    Y +     NG+I+ PAG+
Sbjct: 301 TMMLVATVIATVTFQAGVNPAGGVWQQDTEYNSNNYID--EYYYMTATLKNGTIL-PAGS 360

Query: 361 SIMSVQRPFDYWSYLGMNTISFLASLSVILLIISRFPLKNRIGSWLLAFAMCVAVVSLGL 420
           +IM+ +RP  Y  Y   N +SF+AS+ VILLIISR PLKNR+ SWLL  AMC AVV L L
Sbjct: 361 AIMAYRRPIQYMIYSMANVVSFMASVGVILLIISRVPLKNRVCSWLLVLAMCAAVVFLAL 420

Query: 421 GYFFGVRFISSVTLDSSVPSDSSYSSFTLFSFTLLWYVVVGVVVLWHVIRFQVWMVKSL 462
            +  G   ++   L++     ++   ++L  + L  + +VG+V L+H+IRF VW+VKSL
Sbjct: 421 TFLQGFIMVNLKPLNNLAAGFAARFGYSLALYFL--FGLVGLVGLFHIIRFLVWVVKSL 468

BLAST of Spg023957 vs. NCBI nr
Match: XP_011649355.1 (uncharacterized protein LOC101212496 [Cucumis sativus] >KAE8651928.1 hypothetical protein Csa_005946 [Cucumis sativus])

HSP 1 Score: 497.3 bits (1279), Expect = 1.5e-136
Identity = 288/535 (53.83%), Postives = 366/535 (68.41%), Query Frame = 0

Query: 1   MEENRRDPTTSFNSPDAPNEEITIAMCSVEEK-ITKLYEASKSGSIQTLKTLIQEDPNLI 60
           MEEN R+ T+  NS DA +  + + + S+EE  I KLYEASK G +QTLKT I+E+PNLI
Sbjct: 5   MEENHREITSLLNSSDAQSSHVVVEISSMEEDVIRKLYEASKIGCVQTLKTFIKENPNLI 64

Query: 61  QKTLTSTSNV--ESP-LHVAVSHGHLEFTRLLLDHNPELAAEVDAFQRTPLHLA-SKHGD 120
            K L  TS++  E+P LH++VS GHLEFTRLLLDH P+LA EVDAFQRTPLH+A S +GD
Sbjct: 65  HKVLIYTSSITTETPLLHISVSQGHLEFTRLLLDHIPQLATEVDAFQRTPLHIACSNNGD 124

Query: 121 METIQALLEKNTSACLVYDKNGLIPLHYAVIGGQIEIMLVLINARPQSFWMKL---NNGQ 180
           ME I+ALLEKNTS+CLV D NG IPLHYAVI   IE+M +LI ARPQS  MK    NNG+
Sbjct: 125 MEIIRALLEKNTSSCLVQDLNGFIPLHYAVISENIEMMKLLIKARPQSILMKALHNNNGK 184

Query: 181 TILHLCVENNHLEGIKLLVETFMNRDENFLNTIDDKGNTVLDLSLMLRQSEMVGYLISIP 240
           T+LHLCVE N+LEG+KLL+   +  D++FLNT+DD+GNT+LDLSL LR+ EMVGYL++IP
Sbjct: 185 TVLHLCVEGNYLEGMKLLIPQTLLFDKDFLNTMDDEGNTILDLSLTLRRIEMVGYLLTIP 244

Query: 241 EVKTRTSSRKD---------FAASNGTKESS-----EPQESRGRWKVMRKNLKYKGDWVQ 300
           E KTRT+  K+          A +  TK          ++S G WKV +K LKYKGDWVQ
Sbjct: 245 EAKTRTNDTKEKILESQKITKARNRKTKRRELVSLCTKKKSIGLWKVWKKKLKYKGDWVQ 304

Query: 301 EVQGTMMLVATVIATVTFQAGINPPGGVWQQDTPF----------NCSSHIEW-ISRYNL 360
           EVQGTMMLVATVIATVTFQ G+NPPGGVWQQDTPF          NC +        Y+L
Sbjct: 305 EVQGTMMLVATVIATVTFQGGVNPPGGVWQQDTPFVYSSFNDATKNCLNEFYMNFGLYDL 364

Query: 361 PCFGINGSIIFPAGTSIMSVQRPFDYWSYLGMNTISFLASLSVILLIISRFPLKNRIGSW 420
             +  N +++F AGT +M  Q+P  Y  Y+ +NT+SFLAS++VIL+I+SRFPLKNRI SW
Sbjct: 365 FSYS-NTTVLFAAGTGVMKSQQPEVYSIYIWVNTVSFLASMTVILMIVSRFPLKNRICSW 424

Query: 421 LLAFAMCVAVVSLGLGYFFGVRFISSVTLDSSVPSDSSYSSFTLFSFT------------ 480
           LLAFAMC+AV+SL +GY  GV+ +  +    + P    ++ +++FS T            
Sbjct: 425 LLAFAMCIAVLSLAIGYLLGVKMVHLL----NFPDGRIFAPYSIFSLTIICWLGVVGLVC 484

BLAST of Spg023957 vs. NCBI nr
Match: XP_004141216.1 (uncharacterized protein LOC101203970 [Cucumis sativus] >KGN59794.1 hypothetical protein Csa_000761 [Cucumis sativus])

HSP 1 Score: 492.7 bits (1267), Expect = 3.7e-135
Identity = 277/499 (55.51%), Postives = 351/499 (70.34%), Query Frame = 0

Query: 20  EEITIAMCSVEEKITKLYEASKSGSIQTLKTLIQEDPNLIQKTLTSTSNVESP----LHV 79
           +EI IAM  ++E I KLY A++ G I++LKTLI+EDP +IQK + S+SN        LH+
Sbjct: 8   QEIRIAMSCLQENIRKLYVAAEKGCIESLKTLIEEDPCIIQKVVISSSNNNENRHPLLHL 67

Query: 80  AVSHGHLEFTRLLLDHNPELAAEVDAFQRTPLHLASKHGDMETIQA-LLEKNTSACLVYD 139
           ++S+GHLEFTRLL+ + P+LAAEVD  QRTPLHLASK G+ E ++A LLEKN ++  VYD
Sbjct: 68  SISNGHLEFTRLLIHYEPQLAAEVDLLQRTPLHLASKLGETEIVEALLLEKNMNSYFVYD 127

Query: 140 KNGLIPLHYAVIGGQIEIMLVLINARPQSFWMKL-NNGQTILHLCVENNHLEGIKLLVET 199
            +GLIPLHYAV+ GQ +IM  LI ARP+S WMKL NNGQT+LHLCVE+NHLEG+K L+ET
Sbjct: 128 SDGLIPLHYAVLSGQTDIMQKLIKARPRSLWMKLKNNGQTVLHLCVESNHLEGMKFLIET 187

Query: 200 FMNRDENFLNTIDDKGNTVLDLSLMLRQSEMVGYLISIPEVKTRTSSRKDFAASNGTKES 259
           ++N DE+FLNTIDD GNT+LDLS+ML Q +MVGYL+S  EVKT TS   +  AS+ T ES
Sbjct: 188 YVNDDEDFLNTIDDNGNTILDLSMMLGQRKMVGYLLSALEVKTETSIITNLEASDDTHES 247

Query: 260 SEPQE--------------------SRGRWKVMRKNLKYKGDWVQEVQGTMMLVATVIAT 319
            E Q+                    S+ RW+  R NLKYKGDW QEVQGTMMLVATVIAT
Sbjct: 248 LELQKLSNTRNPRGKKSRKHGLKNTSKLRWRAWRMNLKYKGDWFQEVQGTMMLVATVIAT 307

Query: 320 VTFQAGINPPGGVWQQDTPFNCSSHI------------EWISRYNLPCFGINGSIIFPAG 379
           VTFQAG+NPPGGVWQQDTPFN SS+              + S Y      I    IFPAG
Sbjct: 308 VTFQAGLNPPGGVWQQDTPFNSSSYFNNDPYYRPFNSSSYFSYYPYDSPRIPLGNIFPAG 367

Query: 380 TSIMSVQRPFDYWSYLGMNTISFLASLSVILLIISRFPLKNRIGSWLLAFAMCVAVVSLG 439
           T+IM   +P+ Y  Y+ +NTISFLAS+SVILLI+ RFPLKN+I SWLLA AMCVAVV+LG
Sbjct: 368 TAIMMYFKPYRYSYYMQVNTISFLASISVILLIVGRFPLKNKICSWLLALAMCVAVVTLG 427

Query: 440 LGYFFGVRFISSVTLDSSVPSDSSYSSFTLFSFTLLWYVVVGVVVLWHVIRFQVWMVKSL 481
            GYF GV  ++   ++ S+   S+++S    + + L   +V V  +WH++ F++W+VK+L
Sbjct: 428 NGYFSGVAMVNYSGMNDSL---SAFNSLYFLAISCLG--LVRVTGVWHILSFKLWIVKTL 487

BLAST of Spg023957 vs. NCBI nr
Match: XP_022152683.1 (ankyrin repeat-containing protein BDA1-like [Momordica charantia])

HSP 1 Score: 488.4 bits (1256), Expect = 7.0e-134
Identity = 279/461 (60.52%), Postives = 325/461 (70.50%), Query Frame = 0

Query: 19  NEEITIAMCSVEEKITKLYEASKSGSIQTLKTLIQEDPNLIQKTLTSTSNVESPLHVAVS 78
           N EI I M  VEE   KLYE SK G IQTLKTLIQ+DP LIQK L STSN+ESPLHV+VS
Sbjct: 17  NHEIQIEMSFVEENTEKLYEGSKIGCIQTLKTLIQDDPRLIQKVLVSTSNIESPLHVSVS 76

Query: 79  HGHLEFTRLLLDHNPELAAEVDAFQRTPLHLASKHGDMETIQALLEKNTSACLVYDKNGL 138
           HGHLEFTRLLLDH+PELAAEVDA QRTPLHLAS++GDMETIQALLEKNTSACL YD NGL
Sbjct: 77  HGHLEFTRLLLDHDPELAAEVDASQRTPLHLASENGDMETIQALLEKNTSACLAYDNNGL 136

Query: 139 IPLHYAVIGGQIEIMLVLINARPQSFWMKLNNGQTILHLCVENNHLEGIKLLVETF-MNR 198
           IPLH AVI G+I+IM  LI ARPQS WMKL NGQTILHLCVENNHLE +KLLVETF MN 
Sbjct: 137 IPLHCAVIRGEIKIMQQLIKARPQSIWMKLKNGQTILHLCVENNHLEAMKLLVETFAMNG 196

Query: 199 DENFLNTIDDKGNTVLDLSLMLRQSEMVGYLISIPEVKT------------------RTS 258
           D++ +N IDD GNT+LDLS++LRQ EMVGYL+SIPEVKT                  R  
Sbjct: 197 DKDLVNAIDDAGNTILDLSIVLRQIEMVGYLLSIPEVKTKFVGTKRSSPTPKSIIKRRNR 256

Query: 259 SRKDFAASNGTKESSEPQESRGRW-KVMRKNLKYKGDWVQEVQGTMMLVATVIATVTFQA 318
           + K       T      + S  RW KV R + +YK +W QEVQG MMLVATVIATVTFQA
Sbjct: 257 NLKIMQRRGETASLCSKKRSGRRWGKVWRNDEEYKAEWFQEVQGRMMLVATVIATVTFQA 316

Query: 319 GINPPGGVWQQDTPFNCSSHIEWISRYNLPCFGINGSIIFPAGTSIMSVQRPFDYWSYLG 378
            INPPGGVWQQDT +N SS     S +  P    N   IFPAG++IM+ +    +  YL 
Sbjct: 317 AINPPGGVWQQDTRYNSSSS----SHHYNPT--PNDGTIFPAGSAIMAYRYQEAHLVYLM 376

Query: 379 MNTISFLASLSVILLIISRFPLKNRIGSWLLAFAMCVAVVSLGLGYFFGVRFISSVTLDS 438
           MNT+SFLAS SVILLIISRFPLKN+I SW+L   M  AV  L LGY  G R +S    D 
Sbjct: 377 MNTVSFLASASVILLIISRFPLKNKICSWILTLTMGAAVAFLALGYLLGARLVS--LNDV 436

Query: 439 SVPSDSSYSSFTLFSFTLLWYVVVGVVVLWHVIRFQVWMVK 460
              + S+Y  +++  +  +W+ ++ +V L +V+RF VW+ K
Sbjct: 437 YYTNVSAYIGYSIAFY--VWFGMIALVNLCYVLRFLVWVFK 467

BLAST of Spg023957 vs. ExPASy Swiss-Prot
Match: Q6AWW5 (Ankyrin repeat-containing protein At5g02620 OS=Arabidopsis thaliana OX=3702 GN=At5g02620 PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 1.0e-18
Identity = 85/284 (29.93%), Postives = 125/284 (44.01%), Query Frame = 0

Query: 39  ASKSGSIQTLKTLIQEDPNLIQKTLTSTSNVESPLHVAVSHGHLEFTRLLLDHNPELAAE 98
           A+K+G++Q L  LI+ +P L   + T  S+  + LH A S GH E    LLD   +LAA 
Sbjct: 98  AAKNGNLQVLDVLIEANPEL---SFTFDSSKTTALHTAASQGHGEIVCFLLDKGVDLAAI 157

Query: 99  VDAFQRTPLHLASKHGDMETIQALLEKNTSACLVYDKNGLIPLHYAVIGGQIEIMLVLIN 158
             +  +T LH A+++G    ++ L+EK        DK G   LH AV G   EI+ VL+ 
Sbjct: 158 ARSNGKTALHSAARNGHTVIVKKLIEKKAGMVTRVDKKGQTALHMAVKGQNTEIVDVLME 217

Query: 159 ARPQSFWMKLNNGQTILHLCVENNHLEGIKLLVETFMNRDENFLNTIDDKGNTVLDLSLM 218
           A         N G T LH+ V  N  E    +V+T +   E     ++  G T LD++  
Sbjct: 218 ADGSLINSADNKGNTPLHIAVRKNRAE----IVQTVLKYCEVSRVAVNKSGETALDIAEK 277

Query: 219 LRQSEMVGYLISIPEVKTRTSSRKDFAASNGTKESSEPQESRGRWKVMRKNLKYKGDWVQ 278
               E+V  L  I     R+    +    +G+    +   S    +V    L+  G   +
Sbjct: 278 TGLHEIVPLLQKIGMQNARSIKPAEKVEPSGSSRKLKETVSEIGHEV-HTQLEQTGRTRR 337

Query: 279 EVQG------------------TMMLVATVIATVTFQAGINPPG 305
           E+QG                  +  LVA +IATV F A  N PG
Sbjct: 338 EIQGIAKRVNKMHTEGLNNAINSTTLVAILIATVAFAAIFNVPG 373

BLAST of Spg023957 vs. ExPASy Swiss-Prot
Match: Q8GYH5 (Ankyrin repeat-containing protein BDA1 OS=Arabidopsis thaliana OX=3702 GN=BAD1 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 6.6e-18
Identity = 61/202 (30.20%), Postives = 108/202 (53.47%), Query Frame = 0

Query: 34  TKLYEASKSGSIQTLKTLIQEDPNLIQKTLTSTSNVESPLHVAVSHGHLEFTRLLLDHNP 93
           +KL   ++SGS+  L +LIQ  P+++QK +     + +PLH A S G L+    L+   P
Sbjct: 3   SKLLLVTQSGSVDDLYSLIQAAPDILQK-VDVLPIIHTPLHEASSAGKLDLAMELMILKP 62

Query: 94  ELAAEVDAFQRTPLHLASKHGDMETIQALLEKNTSACLVYDKNGLIPLHYAVIGGQIEIM 153
             A +++ +  +PLHLA ++  +E    L++ + S   +  + G+ PLH     G ++++
Sbjct: 63  SFAKKLNEYGLSPLHLAVENDQVELALELVKVDPSLVRIRGRGGMTPLHLVAKKGDVDLL 122

Query: 154 LVLINARPQSFWMKLNNGQTILHLCVENNHLEGIKLL---VETFMNRDENF---LNTIDD 213
              + A P+S      NG+TILH+ + N+  E +K+L   ++   + D+ F   LN  D 
Sbjct: 123 TDFLLACPESIKDVNVNGETILHITIMNDKYEQLKVLTGWMQKMRDSDDVFIDVLNRRDR 182

Query: 214 KGNTVLDLSLMLRQSEMVGYLI 230
            GNTVL L+      ++V  L+
Sbjct: 183 GGNTVLHLAAYENNDKVVKQLV 203

BLAST of Spg023957 vs. ExPASy Swiss-Prot
Match: P16157 (Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=3)

HSP 1 Score: 85.1 bits (209), Expect = 2.3e-15
Identity = 58/163 (35.58%), Postives = 89/163 (54.60%), Query Frame = 0

Query: 34  TKLYEASKSGSIQTLKTLIQEDPNLIQKTLTSTSNVESPLHVAVSHGHLEFTRLLLDH-- 93
           T L+ A+++G  +  K L+Q       K      + ++PLH A   GH    +LLL++  
Sbjct: 439 TPLHMAARAGHTEVAKYLLQNK----AKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNA 498

Query: 94  NPELAAEVDAFQRTPLHLASKHGDMETIQALLEKNTS-ACLVYDKNGLIPLHYAVIGGQI 153
           NP LA        TPLH+A++ G +ET+ ALLEK  S AC+   K G  PLH A   G++
Sbjct: 499 NPNLATTAG---HTPLHIAAREGHVETVLALLEKEASQACMT--KKGFTPLHVAAKYGKV 558

Query: 154 EIMLVLI--NARPQSFWMKLNNGQTILHLCVENNHLEGIKLLV 192
            +  +L+  +A P +      NG T LH+ V +N+L+ +KLL+
Sbjct: 559 RVAELLLERDAHPNAAG---KNGLTPLHVAVHHNNLDIVKLLL 589

BLAST of Spg023957 vs. ExPASy Swiss-Prot
Match: Q9ZU96 (Ankyrin repeat-containing protein At2g01680 OS=Arabidopsis thaliana OX=3702 GN=At2g01680 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.5e-14
Identity = 51/162 (31.48%), Postives = 78/162 (48.15%), Query Frame = 0

Query: 74  HVAVSHGHLEFTRLLLDHNPELAAEVDAFQRTPLHLASKHGDMETIQALLEKNTSACLVY 133
           HVA   GHL   + LL   PEL    DA   +PL+ A+    +E + A+L+ + S  ++ 
Sbjct: 99  HVAAKRGHLGIVKELLRLWPELCRICDASNTSPLYAAAVQDHLEIVNAMLDVDPSCAMIV 158

Query: 134 DKNGLIPLHYAVIGGQIEIMLVLINARPQSFWMKLNNGQTILHLCVENNHLEGIKLLVET 193
            KNG   LH A   G + I+  LI        +K   GQT LH+ V+   LE    +VE 
Sbjct: 159 RKNGKTSLHTAGRYGLLRIVKALIEKDAAIVGVKDKKGQTALHMAVKGRSLE----VVEE 218

Query: 194 FMNRDENFLNTIDDKGNTVLDLSLMLRQSEMVGYLISIPEVK 236
            +  D   LN  D KGNT L ++    + ++   L++   ++
Sbjct: 219 ILQADYTILNERDRKGNTALHIATRKARPQITSLLLTFTAIE 256

BLAST of Spg023957 vs. ExPASy Swiss-Prot
Match: P0DJE3 (Alpha-latrotoxin-Lhe1a OS=Latrodectus hesperus OX=256737 PE=1 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 7.5e-14
Identity = 50/170 (29.41%), Postives = 80/170 (47.06%), Query Frame = 0

Query: 71  SPLHVAVSHGHLEFTRLLLDHNPELAAEVDAFQRTPLHLASKHGDMETIQALLEKNTSAC 130
           +P+HVA   G+     LL+     + ++   F +TPLHLA++ G + T Q L+E +    
Sbjct: 528 TPIHVAADSGNAGIVNLLIQRGVSINSKTYHFLQTPLHLAAQRGFVNTFQRLMESSEINI 587

Query: 131 LVYDKNGLIPLHYAVIGGQIEIMLVLINARPQSFWMKLNNGQTILHLCVENNHLEGIKLL 190
              DK+G  PLHYAV GG+  I+   +N        K N G T  HL +  N  +    +
Sbjct: 588 NERDKDGFTPLHYAVRGGE-RILEAFMNQIGIDVNAKSNKGLTPFHLAIIKNDWQ----V 647

Query: 191 VETFMNRDENFLNTIDDKGNTVLDLSLMLRQSEMVGYLISIPEVKTRTSS 241
             T +   +  +N +D+   T L  + +L   E    LI++ E+     S
Sbjct: 648 ASTLLRNKKVDINAVDENNMTALHYAAILGYLETTKQLINLKEINANVVS 692

BLAST of Spg023957 vs. ExPASy TrEMBL
Match: A0A1S3BIS1 (uncharacterized protein LOC103490026 OS=Cucumis melo OX=3656 GN=LOC103490026 PE=4 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 5.0e-146
Identity = 296/513 (57.70%), Postives = 368/513 (71.73%), Query Frame = 0

Query: 1   MEENRRDPTTSFNSPDAPNEEITIAMCSVEEK-ITKLYEASKSGSIQTLKTLIQEDPNLI 60
           MEEN ++ T+ FNS DA N ++ I M S+EE  I KLYEASK G +QTLKTLIQ++P+LI
Sbjct: 1   MEENHQEITSLFNSSDAQNSQVIIEMSSMEEDIIRKLYEASKIGCVQTLKTLIQDNPDLI 60

Query: 61  QKTL--TSTSNVESP-LHVAVSHGHLEFTRLLLDHNPELAAEVDAFQRTPLHLA-SKHGD 120
            K L  TS+  +E+P LHV+V HGHLEFT+LLLDHNP+LAAEVDAFQRTPLH+A S +GD
Sbjct: 61  HKALIYTSSITIETPLLHVSVFHGHLEFTQLLLDHNPQLAAEVDAFQRTPLHIACSNNGD 120

Query: 121 METIQALLEKNTSACLVYDKNGLIPLHYAVIGGQIEIMLVLINARPQSFWMKLNNG--QT 180
           ME I+ALLEKNTSACLV D +GLIPLHYAVI   IE+M +LI ARPQS  MKLNN   +T
Sbjct: 121 MEIIRALLEKNTSACLVQDLSGLIPLHYAVISENIEMMELLIKARPQSVLMKLNNNNDKT 180

Query: 181 ILHLCVENNHLEGIKLLVETFMNRDENFLNTIDDKGNTVLDLSLMLRQSEMVGYLISIPE 240
           +LHLCVE NHLEG+KLL+   +  D++FLN +DD+GNT+LDLSLMLR+ EMVGYL+ IPE
Sbjct: 181 VLHLCVEGNHLEGMKLLIAQTLLFDKDFLNAMDDEGNTILDLSLMLRRIEMVGYLLMIPE 240

Query: 241 VKTRTSSRKD--FAASNGTKESSEPQESR------------GRWKVMRKNLKYKGDWVQE 300
            KTRT+  K+    +   TK  +   + R            GRWKV RK LKY+GDWVQE
Sbjct: 241 AKTRTNDIKEKLLESQKITKTRNRKTQRRESVSLSTKKRPIGRWKVWRKKLKYRGDWVQE 300

Query: 301 VQGTMMLVATVIATVTFQAGINPPGGVWQQDTPFNCSSHI--------EWISRYNL---- 360
           VQGTMMLVATVIATVTFQ G+NPPGGVWQQDTPF  SS I        EW   + L    
Sbjct: 301 VQGTMMLVATVIATVTFQGGVNPPGGVWQQDTPFIYSSIINTTKNGFSEWYKDFGLYEEY 360

Query: 361 PCFGINGSIIFPAGTSIMSVQRPFDYWSYLGMNTISFLASLSVILLIISRFPLKNRIGSW 420
                N S++FPAGT +M  Q+P     YL +NT+SFLAS+SVIL+I+SRFPLKNRI SW
Sbjct: 361 SYLRNNTSVLFPAGTGVMRFQQPLLCSLYLWVNTVSFLASMSVILMIVSRFPLKNRICSW 420

Query: 421 LLAFAMCVAVVSLGLGYFFGVRFISSVTLDSSVPSDSSYSSFTLFSFTLL-WYVVVGVVV 480
           LL   MC+AVVSL +GY  GV+ ++ +T     P    +S+  +FS T++ W+ +VG+V 
Sbjct: 421 LLTLDMCIAVVSLAIGYLLGVKMVNLLTF----PEKEDFSNSDVFSLTVICWFGIVGLVC 480

BLAST of Spg023957 vs. ExPASy TrEMBL
Match: A0A0A0LFQ3 (ANK_REP_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G846590 PE=4 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.8e-135
Identity = 277/499 (55.51%), Postives = 351/499 (70.34%), Query Frame = 0

Query: 20  EEITIAMCSVEEKITKLYEASKSGSIQTLKTLIQEDPNLIQKTLTSTSNVESP----LHV 79
           +EI IAM  ++E I KLY A++ G I++LKTLI+EDP +IQK + S+SN        LH+
Sbjct: 8   QEIRIAMSCLQENIRKLYVAAEKGCIESLKTLIEEDPCIIQKVVISSSNNNENRHPLLHL 67

Query: 80  AVSHGHLEFTRLLLDHNPELAAEVDAFQRTPLHLASKHGDMETIQA-LLEKNTSACLVYD 139
           ++S+GHLEFTRLL+ + P+LAAEVD  QRTPLHLASK G+ E ++A LLEKN ++  VYD
Sbjct: 68  SISNGHLEFTRLLIHYEPQLAAEVDLLQRTPLHLASKLGETEIVEALLLEKNMNSYFVYD 127

Query: 140 KNGLIPLHYAVIGGQIEIMLVLINARPQSFWMKL-NNGQTILHLCVENNHLEGIKLLVET 199
            +GLIPLHYAV+ GQ +IM  LI ARP+S WMKL NNGQT+LHLCVE+NHLEG+K L+ET
Sbjct: 128 SDGLIPLHYAVLSGQTDIMQKLIKARPRSLWMKLKNNGQTVLHLCVESNHLEGMKFLIET 187

Query: 200 FMNRDENFLNTIDDKGNTVLDLSLMLRQSEMVGYLISIPEVKTRTSSRKDFAASNGTKES 259
           ++N DE+FLNTIDD GNT+LDLS+ML Q +MVGYL+S  EVKT TS   +  AS+ T ES
Sbjct: 188 YVNDDEDFLNTIDDNGNTILDLSMMLGQRKMVGYLLSALEVKTETSIITNLEASDDTHES 247

Query: 260 SEPQE--------------------SRGRWKVMRKNLKYKGDWVQEVQGTMMLVATVIAT 319
            E Q+                    S+ RW+  R NLKYKGDW QEVQGTMMLVATVIAT
Sbjct: 248 LELQKLSNTRNPRGKKSRKHGLKNTSKLRWRAWRMNLKYKGDWFQEVQGTMMLVATVIAT 307

Query: 320 VTFQAGINPPGGVWQQDTPFNCSSHI------------EWISRYNLPCFGINGSIIFPAG 379
           VTFQAG+NPPGGVWQQDTPFN SS+              + S Y      I    IFPAG
Sbjct: 308 VTFQAGLNPPGGVWQQDTPFNSSSYFNNDPYYRPFNSSSYFSYYPYDSPRIPLGNIFPAG 367

Query: 380 TSIMSVQRPFDYWSYLGMNTISFLASLSVILLIISRFPLKNRIGSWLLAFAMCVAVVSLG 439
           T+IM   +P+ Y  Y+ +NTISFLAS+SVILLI+ RFPLKN+I SWLLA AMCVAVV+LG
Sbjct: 368 TAIMMYFKPYRYSYYMQVNTISFLASISVILLIVGRFPLKNKICSWLLALAMCVAVVTLG 427

Query: 440 LGYFFGVRFISSVTLDSSVPSDSSYSSFTLFSFTLLWYVVVGVVVLWHVIRFQVWMVKSL 481
            GYF GV  ++   ++ S+   S+++S    + + L   +V V  +WH++ F++W+VK+L
Sbjct: 428 NGYFSGVAMVNYSGMNDSL---SAFNSLYFLAISCLG--LVRVTGVWHILSFKLWIVKTL 487

BLAST of Spg023957 vs. ExPASy TrEMBL
Match: A0A6J1DFI1 (ankyrin repeat-containing protein BDA1-like OS=Momordica charantia OX=3673 GN=LOC111020343 PE=4 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 3.4e-134
Identity = 279/461 (60.52%), Postives = 325/461 (70.50%), Query Frame = 0

Query: 19  NEEITIAMCSVEEKITKLYEASKSGSIQTLKTLIQEDPNLIQKTLTSTSNVESPLHVAVS 78
           N EI I M  VEE   KLYE SK G IQTLKTLIQ+DP LIQK L STSN+ESPLHV+VS
Sbjct: 17  NHEIQIEMSFVEENTEKLYEGSKIGCIQTLKTLIQDDPRLIQKVLVSTSNIESPLHVSVS 76

Query: 79  HGHLEFTRLLLDHNPELAAEVDAFQRTPLHLASKHGDMETIQALLEKNTSACLVYDKNGL 138
           HGHLEFTRLLLDH+PELAAEVDA QRTPLHLAS++GDMETIQALLEKNTSACL YD NGL
Sbjct: 77  HGHLEFTRLLLDHDPELAAEVDASQRTPLHLASENGDMETIQALLEKNTSACLAYDNNGL 136

Query: 139 IPLHYAVIGGQIEIMLVLINARPQSFWMKLNNGQTILHLCVENNHLEGIKLLVETF-MNR 198
           IPLH AVI G+I+IM  LI ARPQS WMKL NGQTILHLCVENNHLE +KLLVETF MN 
Sbjct: 137 IPLHCAVIRGEIKIMQQLIKARPQSIWMKLKNGQTILHLCVENNHLEAMKLLVETFAMNG 196

Query: 199 DENFLNTIDDKGNTVLDLSLMLRQSEMVGYLISIPEVKT------------------RTS 258
           D++ +N IDD GNT+LDLS++LRQ EMVGYL+SIPEVKT                  R  
Sbjct: 197 DKDLVNAIDDAGNTILDLSIVLRQIEMVGYLLSIPEVKTKFVGTKRSSPTPKSIIKRRNR 256

Query: 259 SRKDFAASNGTKESSEPQESRGRW-KVMRKNLKYKGDWVQEVQGTMMLVATVIATVTFQA 318
           + K       T      + S  RW KV R + +YK +W QEVQG MMLVATVIATVTFQA
Sbjct: 257 NLKIMQRRGETASLCSKKRSGRRWGKVWRNDEEYKAEWFQEVQGRMMLVATVIATVTFQA 316

Query: 319 GINPPGGVWQQDTPFNCSSHIEWISRYNLPCFGINGSIIFPAGTSIMSVQRPFDYWSYLG 378
            INPPGGVWQQDT +N SS     S +  P    N   IFPAG++IM+ +    +  YL 
Sbjct: 317 AINPPGGVWQQDTRYNSSSS----SHHYNPT--PNDGTIFPAGSAIMAYRYQEAHLVYLM 376

Query: 379 MNTISFLASLSVILLIISRFPLKNRIGSWLLAFAMCVAVVSLGLGYFFGVRFISSVTLDS 438
           MNT+SFLAS SVILLIISRFPLKN+I SW+L   M  AV  L LGY  G R +S    D 
Sbjct: 377 MNTVSFLASASVILLIISRFPLKNKICSWILTLTMGAAVAFLALGYLLGARLVS--LNDV 436

Query: 439 SVPSDSSYSSFTLFSFTLLWYVVVGVVVLWHVIRFQVWMVK 460
              + S+Y  +++  +  +W+ ++ +V L +V+RF VW+ K
Sbjct: 437 YYTNVSAYIGYSIAFY--VWFGMIALVNLCYVLRFLVWVFK 467

BLAST of Spg023957 vs. ExPASy TrEMBL
Match: A0A1S3CM14 (uncharacterized protein LOC103501999 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501999 PE=4 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 2.2e-133
Identity = 270/493 (54.77%), Postives = 342/493 (69.37%), Query Frame = 0

Query: 20  EEITIAMCSVEEKITKLYEASKSGSIQTLKTLIQEDPNLIQKTL-TSTSNVESP----LH 79
           +EI IAM S+EE   KLYE ++ G I++LK L++EDP +IQ  + TS+SN        LH
Sbjct: 8   KEIRIAMSSIEENTRKLYEVTEKGCIESLKILMEEDPYIIQTVVSTSSSNNNETHLPLLH 67

Query: 80  VAVSHGHLEFTRLLLDHNPELAAEVDAFQRTPLHLASKHGDMETIQALLEKNTSACLVYD 139
           +++S GHLEFTRLL+ + P+LAAEVD+ QRTPLHLASK G ME ++ALLEKN SAC VYD
Sbjct: 68  LSISSGHLEFTRLLIHYKPQLAAEVDSLQRTPLHLASKLGKMEIVEALLEKNMSACFVYD 127

Query: 140 KNGLIPLHYAVIGGQIEIMLVLINARPQSFWMKL-NNGQTILHLCVENNHLEGIKLLVET 199
            +G+IPLHYAV+ GQ +++  L+  RP+S WMKL NNGQT+LHLCVE+NHLE +K L+ET
Sbjct: 128 SDGMIPLHYAVLSGQTDVIQKLVKVRPRSLWMKLKNNGQTVLHLCVESNHLEVMKFLIET 187

Query: 200 FMNRDENFLNTIDDKGNTVLDLSLMLRQSEMVGYLISIPEVKTRTSSRKDFAASNGTKES 259
           ++N DE+FLN+IDD GNT+LDLS++LRQ +MVGYL+S  EVKT+TS   +F  S+GT ES
Sbjct: 188 YVNDDEDFLNSIDDHGNTILDLSMLLRQRKMVGYLLSALEVKTKTSIITNFETSDGTNES 247

Query: 260 SEPQE--------------------SRGRWKVMRKNLKYKGDWVQEVQGTMMLVATVIAT 319
            E QE                    ++ RW+  R NLKYKG W QEVQGTMMLVATVIAT
Sbjct: 248 LELQEISKTRNPKRQKSGKHGSKKTTKPRWRAWRTNLKYKGGWFQEVQGTMMLVATVIAT 307

Query: 320 VTFQAGINPPGGVWQQDTPFNCSSHIEWISR-YNLPCFGING-----SIIFPAGTSIMSV 379
           VTFQAG+NPPGGVWQ DTPFN S +     R +N   +   G     SI   AGT+IM  
Sbjct: 308 VTFQAGVNPPGGVWQPDTPFNSSYYFNNDPRSFNSSSYFDYGPYYIPSIQLTAGTAIMMY 367

Query: 380 QRPFDYWSYLGMNTISFLASLSVILLIISRFPLKNRIGSWLLAFAMCVAVVSLGLGYFFG 439
             P  Y +Y  MNTISFLAS+SVILL++ RFPLKN+I SWLLA AM VAV +LG GYF G
Sbjct: 368 LEPDKYTAYTHMNTISFLASISVILLVVGRFPLKNKICSWLLALAMSVAVAALGQGYFLG 427

Query: 440 VRFISSVTLDSSVPSDSSYSSFTLFSFTLLWYVVVGVVVLWHVIRFQVWMVKSLFRTFTS 481
           V  ++  +  +   S S Y  + L    L    V GV   WH++ F++W+VK+LFRTFT+
Sbjct: 428 VEMVNKYSYFNDSFSGSFYLLYLLVISCLGLVRVAGV---WHILSFKLWIVKTLFRTFTT 487

BLAST of Spg023957 vs. ExPASy TrEMBL
Match: A0A0A0LCQ0 (ANK_REP_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G847090 PE=4 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 6.4e-133
Identity = 278/511 (54.40%), Postives = 350/511 (68.49%), Query Frame = 0

Query: 1   MEENRRDPTTSFNSPDAPNEEITIAMCSVEEKITKLYEASKSGSIQTLKTLIQEDPNLIQ 60
           ME N ++ T   +S DA N ++ I+M  VEE ITKLYEASK G ++TLKTLIQ+ P LIQ
Sbjct: 1   MEYNHQEITQLLSSSDAENRQVVISMSVVEEDITKLYEASKIGCVETLKTLIQQHPYLIQ 60

Query: 61  KTLTSTSNVESP-LHVAVSHGHLEFTRLLLDHNPELAAEVDAFQRTPLHLASKHGDMETI 120
           K   S   +E+P LHV+VSHG+LEFT++LL+HNP+LAAEVD +QRTPLH+A  +G +E +
Sbjct: 61  K--ASIYTIETPLLHVSVSHGYLEFTQVLLNHNPQLAAEVDVYQRTPLHIACANGCIEMV 120

Query: 121 QALLEKNTSACLVYDKNGLIPLHYAVIGGQIEIMLVLINARPQSFWMKLNNGQTILHLCV 180
           +A+LEKNTSACLV D NG IPLHYAV  G IE+M +LINARPQS  MKLNNG+T+LHLCV
Sbjct: 121 RAMLEKNTSACLVEDHNGFIPLHYAVTRGNIEMMELLINARPQSILMKLNNGKTVLHLCV 180

Query: 181 ENNHLEGIKLLVETFMNRDENFLNTIDDKGNTVLDLSLMLRQSEMVGYLISIPEVKTRTS 240
           E NHLEG+KLL+   +   E+FLNT+DD GNT+LDLS+MLR+ EMVGYL++IPEV TRT 
Sbjct: 181 EGNHLEGLKLLIAQTLLLFEDFLNTVDDVGNTILDLSVMLRRIEMVGYLLTIPEVNTRT- 240

Query: 241 SRKDFAASNGTKE------------SSEPQESRGRWKV----------MRKNLKYKGDWV 300
           S  DF++SN  K               + +ES   W            M K L+Y+GDWV
Sbjct: 241 SMTDFSSSNRRKRLQSRKITITKSLQRQRRESISLWTTKKLKRRTFDKMSKKLEYQGDWV 300

Query: 301 QEVQGTMMLVATVIATVTFQAGINPPGGVWQQDTPFNCS-------SHIEW---ISRYNL 360
            EVQ TMMLVATVIATVTFQ G+NPPGG+WQQDT FN S       S  +W   +S Y+ 
Sbjct: 301 HEVQDTMMLVATVIATVTFQGGVNPPGGIWQQDTSFNYSDFNNSTNSWNQWFKSLSLYDD 360

Query: 361 PCFGING----SIIFPAGTSIMSVQRPFDYWSYLGMNTISFLASLSVILLIISRFPLKNR 420
               IN     +++FPAGT +M  Q+P  YW YL +NTISFLAS+SVIL+I+ RFPLKNR
Sbjct: 361 LTNTINPNNNLTVLFPAGTGVMGYQQPQIYWIYLCVNTISFLASVSVILMIVGRFPLKNR 420

Query: 421 IGSWLLAFAMCVAVVSLGLGYFFGVRFISSVTLDSSVPSDSSYSSF--TLFSFTLLWYVV 473
           I SW+L+  MC AVVSL +GY  GV+ I+ + ++  +     ++ F   L S    W  V
Sbjct: 421 IFSWILSLTMCTAVVSLAIGYLIGVKMINLMAIEDYI----KFNEFDNVLPSTVFCWLGV 480

BLAST of Spg023957 vs. TAIR 10
Match: AT5G15500.2 (Ankyrin repeat family protein )

HSP 1 Score: 102.1 bits (253), Expect = 1.3e-21
Identity = 84/311 (27.01%), Postives = 144/311 (46.30%), Query Frame = 0

Query: 36  LYEASKSGSIQTLKTLIQEDPNLIQKTLTSTSNVESPLHVAVSHGHLEFTRLLLDHNPEL 95
           L  A+KSG+I  L  LI EDP ++ KT      V +PLHVA  +G  EF   +++  P  
Sbjct: 6   LEAAAKSGNIDLLYELIHEDPYVLDKT-DHVPFVNTPLHVAAVNGKTEFAMEMMNLKPSF 65

Query: 96  AAEVDAFQRTPLHLASKHGDMETIQALLEKNTSACLVYDKNGLIPLHYAVIGGQIEIMLV 155
           A +++A   TPLHLA +HG    +  +++ + S   +  ++G+ PL  AV   +I++M  
Sbjct: 66  ARKLNADGLTPLHLAVEHGHFWLVLEVVKVDPSLVRIKGRHGMTPLLVAVSRKKIDLMSE 125

Query: 156 LINARPQSFWMKLNNGQTILHLCVEN-NHLEGIKLL------VETFMNRDENF-----LN 215
                P+S      NG+  LH+ V N +  EG+ +L      +     +D  +     +N
Sbjct: 126 FFLGCPESIVDANVNGENALHIAVNNYDQREGLSVLKVLMGWILRLCQKDAEWIETRVIN 185

Query: 216 TIDDKGNTVLDLSLMLRQSEMVGYLISIPEVKTRTSSRK-----DFAASNGTKE------ 275
             D  GNT L L+      + +  L+   ++     ++      D A  +  +E      
Sbjct: 186 RRDKDGNTPLHLAAYEINRQAMKLLLESSKINVNIENKNGLTVFDIAVLHNNREIERMVK 245

Query: 276 ---------------SSEPQESRGRWKVMRKNLKYK-GDWV-QEVQGTMMLVATVIATVT 307
                          +S+   S+  W+  R+  K +   W+ +E +  +++VAT+I T T
Sbjct: 246 RHGGKRSVSLVKIKTTSDILASQLSWRESRRTKKIRFYSWISEERRNALLVVATLIVTAT 305

BLAST of Spg023957 vs. TAIR 10
Match: AT5G02620.1 (ankyrin-like1 )

HSP 1 Score: 96.3 bits (238), Expect = 7.2e-20
Identity = 85/284 (29.93%), Postives = 125/284 (44.01%), Query Frame = 0

Query: 39  ASKSGSIQTLKTLIQEDPNLIQKTLTSTSNVESPLHVAVSHGHLEFTRLLLDHNPELAAE 98
           A+K+G++Q L  LI+ +P L   + T  S+  + LH A S GH E    LLD   +LAA 
Sbjct: 98  AAKNGNLQVLDVLIEANPEL---SFTFDSSKTTALHTAASQGHGEIVCFLLDKGVDLAAI 157

Query: 99  VDAFQRTPLHLASKHGDMETIQALLEKNTSACLVYDKNGLIPLHYAVIGGQIEIMLVLIN 158
             +  +T LH A+++G    ++ L+EK        DK G   LH AV G   EI+ VL+ 
Sbjct: 158 ARSNGKTALHSAARNGHTVIVKKLIEKKAGMVTRVDKKGQTALHMAVKGQNTEIVDVLME 217

Query: 159 ARPQSFWMKLNNGQTILHLCVENNHLEGIKLLVETFMNRDENFLNTIDDKGNTVLDLSLM 218
           A         N G T LH+ V  N  E    +V+T +   E     ++  G T LD++  
Sbjct: 218 ADGSLINSADNKGNTPLHIAVRKNRAE----IVQTVLKYCEVSRVAVNKSGETALDIAEK 277

Query: 219 LRQSEMVGYLISIPEVKTRTSSRKDFAASNGTKESSEPQESRGRWKVMRKNLKYKGDWVQ 278
               E+V  L  I     R+    +    +G+    +   S    +V    L+  G   +
Sbjct: 278 TGLHEIVPLLQKIGMQNARSIKPAEKVEPSGSSRKLKETVSEIGHEV-HTQLEQTGRTRR 337

Query: 279 EVQG------------------TMMLVATVIATVTFQAGINPPG 305
           E+QG                  +  LVA +IATV F A  N PG
Sbjct: 338 EIQGIAKRVNKMHTEGLNNAINSTTLVAILIATVAFAAIFNVPG 373

BLAST of Spg023957 vs. TAIR 10
Match: AT5G54610.1 (ankyrin )

HSP 1 Score: 93.6 bits (231), Expect = 4.7e-19
Identity = 61/202 (30.20%), Postives = 108/202 (53.47%), Query Frame = 0

Query: 34  TKLYEASKSGSIQTLKTLIQEDPNLIQKTLTSTSNVESPLHVAVSHGHLEFTRLLLDHNP 93
           +KL   ++SGS+  L +LIQ  P+++QK +     + +PLH A S G L+    L+   P
Sbjct: 3   SKLLLVTQSGSVDDLYSLIQAAPDILQK-VDVLPIIHTPLHEASSAGKLDLAMELMILKP 62

Query: 94  ELAAEVDAFQRTPLHLASKHGDMETIQALLEKNTSACLVYDKNGLIPLHYAVIGGQIEIM 153
             A +++ +  +PLHLA ++  +E    L++ + S   +  + G+ PLH     G ++++
Sbjct: 63  SFAKKLNEYGLSPLHLAVENDQVELALELVKVDPSLVRIRGRGGMTPLHLVAKKGDVDLL 122

Query: 154 LVLINARPQSFWMKLNNGQTILHLCVENNHLEGIKLL---VETFMNRDENF---LNTIDD 213
              + A P+S      NG+TILH+ + N+  E +K+L   ++   + D+ F   LN  D 
Sbjct: 123 TDFLLACPESIKDVNVNGETILHITIMNDKYEQLKVLTGWMQKMRDSDDVFIDVLNRRDR 182

Query: 214 KGNTVLDLSLMLRQSEMVGYLI 230
            GNTVL L+      ++V  L+
Sbjct: 183 GGNTVLHLAAYENNDKVVKQLV 203

BLAST of Spg023957 vs. TAIR 10
Match: AT3G13950.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G13266.1); Has 339 Blast hits to 265 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 339; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 92.8 bits (229), Expect = 8.0e-19
Identity = 72/215 (33.49%), Postives = 112/215 (52.09%), Query Frame = 0

Query: 251 KESSEPQESRGRWKVMRKNLKYKGDWVQEVQGTMMLVATVIATVTFQAGINPPGGVWQQD 310
           +E    +ES   W V  K LK +GDW+++ +G +M+ ATVIA ++FQ  +NPPGGVWQ D
Sbjct: 6   QEGHRYEESSSEWFV--KYLKRQGDWLEKTRGNLMVTATVIAGMSFQVMVNPPGGVWQSD 65

Query: 311 TPFNCSSHIEWISRYNLPCFG-INGSIIF---PAGTSIMSVQRPFDYWSYLGM---NTIS 370
              NCS             FG   G+  F    AGT+++  +      +Y+GM   +T+S
Sbjct: 66  ---NCS-------------FGNQTGTAPFCKGRAGTAVLEYESS-KRIAYIGMIISSTVS 125

Query: 371 FLASLSVILLIISRFPLKNRIGSWLLAFAMCVAVVSLGLGYFFGVRFISSVTLDSSVPSD 430
           F  S+S+ILL+IS   L+NR+   +L   M VAV+ +   +FF +           V SD
Sbjct: 126 FSTSMSLILLVISGIRLRNRMIMAILGTFMVVAVLCISAAFFFAIVL---------VQSD 185

Query: 431 SSYSSFTLFSFTLLWYVVVGVVVLWHVIRFQVWMV 459
                + L  +   W V   +++L  ++RF  W++
Sbjct: 186 DQIIRYILLIYVGFWVVFPVLILLIQLVRFIGWLI 192

BLAST of Spg023957 vs. TAIR 10
Match: AT1G14480.2 (Ankyrin repeat family protein )

HSP 1 Score: 92.4 bits (228), Expect = 1.0e-18
Identity = 77/281 (27.40%), Postives = 138/281 (49.11%), Query Frame = 0

Query: 35  KLYEASKSGSIQTLKTLIQEDPNLIQKTLTSTSNVESPLHVAVSHGHLEFTRLLLDHNPE 94
           +L +A++SGSI  L  LI E+P +++  + +   V +PLHVA   G++EF   +L+  P 
Sbjct: 4   RLQQAAESGSINELYALIDENPYILE-NIDAVPFVSTPLHVAAVFGNIEFAMEMLNLKPS 63

Query: 95  LAAEVDAFQRTPLHLASKHGDMETIQALLEKNTSACLVYDKNGLIPLHYAVIGGQIEIML 154
            A +++    +PLHLA +    + +  +L  +     V  +NG+ P H  VI G  +++ 
Sbjct: 64  FARKLNTSGYSPLHLAVEKEQSDFVSHMLWHDGGLSRVKGRNGVTPFHLLVIRGDDDLVA 123

Query: 155 VLINARPQSFWMKLNNGQTILHLCVENNH---LEGIKLLVETFMNRDENFLNTIDDKGNT 214
             +   P+       + Q  LHL V N+    L+ +KLL++  +  + N +N IDD   T
Sbjct: 124 ECLITSPECIEDVNVDRQNALHLAVMNDRFEVLQALKLLLKCRL-VEPNLVN-IDDL--T 183

Query: 215 VLDLSLMLRQSEMVGYLISIPEVKTRTSSRKDFAASNGTKESSEPQESRGRWKVMRKN-- 274
            +D+ L  +     G  + + +   +T    + A+    KE S+  +S   +        
Sbjct: 184 FVDI-LRTQGENAGGGNLDLEQAVIKTGC-VEAASMPKFKEESDLLKSPINFMTYYSTSM 243

Query: 275 LKYKGDWVQEVQGTMMLVATVIATVTFQAGINPPGGVWQQD 311
            + K     + +G  ++V T+I T T+Q  + PPGGV Q +
Sbjct: 244 KRMKSSTSDQDRGAFLIVCTLIITATYQMALQPPGGVHQSE 277

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008447612.11.0e-14557.70PREDICTED: uncharacterized protein LOC103490026 [Cucumis melo][more]
XP_023526885.14.2e-13958.87ankyrin repeat-containing protein NPR4-like [Cucurbita pepo subsp. pepo][more]
XP_011649355.11.5e-13653.83uncharacterized protein LOC101212496 [Cucumis sativus] >KAE8651928.1 hypothetica... [more]
XP_004141216.13.7e-13555.51uncharacterized protein LOC101203970 [Cucumis sativus] >KGN59794.1 hypothetical ... [more]
XP_022152683.17.0e-13460.52ankyrin repeat-containing protein BDA1-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q6AWW51.0e-1829.93Ankyrin repeat-containing protein At5g02620 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q8GYH56.6e-1830.20Ankyrin repeat-containing protein BDA1 OS=Arabidopsis thaliana OX=3702 GN=BAD1 P... [more]
P161572.3e-1535.58Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=3[more]
Q9ZU961.5e-1431.48Ankyrin repeat-containing protein At2g01680 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
P0DJE37.5e-1429.41Alpha-latrotoxin-Lhe1a OS=Latrodectus hesperus OX=256737 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S3BIS15.0e-14657.70uncharacterized protein LOC103490026 OS=Cucumis melo OX=3656 GN=LOC103490026 PE=... [more]
A0A0A0LFQ31.8e-13555.51ANK_REP_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G846... [more]
A0A6J1DFI13.4e-13460.52ankyrin repeat-containing protein BDA1-like OS=Momordica charantia OX=3673 GN=LO... [more]
A0A1S3CM142.2e-13354.77uncharacterized protein LOC103501999 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0LCQ06.4e-13354.40ANK_REP_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G847... [more]
Match NameE-valueIdentityDescription
AT5G15500.21.3e-2127.01Ankyrin repeat family protein [more]
AT5G02620.17.2e-2029.93ankyrin-like1 [more]
AT5G54610.14.7e-1930.20ankyrin [more]
AT3G13950.18.0e-1933.49unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G14480.21.0e-1827.40Ankyrin repeat family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 68..97
e-value: 0.001
score: 28.3
coord: 136..165
e-value: 0.97
score: 18.5
coord: 208..237
e-value: 1900.0
score: 2.9
coord: 102..131
e-value: 0.012
score: 24.8
coord: 170..199
e-value: 0.0092
score: 25.2
coord: 31..60
e-value: 580.0
score: 6.8
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 68..100
score: 10.15197
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 102..134
score: 9.45751
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 135..192
e-value: 5.6E-10
score: 39.7
coord: 36..127
e-value: 1.9E-12
score: 47.6
IPR026961PGG domainPFAMPF13962PGGcoord: 275..408
e-value: 4.7E-19
score: 68.3
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 29..248
e-value: 6.7E-38
score: 132.1
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILY48403Ankyrin repeatcoord: 32..231
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 240..261
NoneNo IPR availablePANTHERPTHR24128HOMEOBOX PROTEIN WARIAIcoord: 38..441
NoneNo IPR availablePANTHERPTHR24128:SF61ANKYRIN REPEAT-CONTAINING PROTEIN ITN1-LIKEcoord: 38..441
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 68..95
score: 9.569101
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 102..128
score: 9.383383

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg023957.1Spg023957.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0005515 protein binding