Spg023745 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg023745
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pyruvate kinase
Location	scaffold13: 3470341 .. 3474548 (+)
RNA-Seq Expression	Spg023745
Synteny	Spg023745

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCTCATCCGATCCTTTCCGCCCTCGATCTTCCGGCGGCAATTCCGAAGCTTTCAACCTCCTCTTCTTTGGCATCTCCGTCTCTGTCTCTGCTTTAATCATCTTCTCCATTTCTCAATTCCTCCTCCAATTCTCCAAATCCCATTTCCCCTTCACAATGACACAGTCTCTGCAGCTTTTCTCCTCCTCCTCCACCCGCTCTCCGCCCATCTCTCTCCCCAAACATTCCCTCTCTAAACCCTCCCCTTCCTCCGCCGCCTTCCGCTTCCCCTTCTCCTTCACCAAATCCTCAATCAGAGCCTCTTCCTCCCCGGATCTCACTTCCCCAGTCCTCGTTTCTGCCAATGGCTCCAGTTCCGGAGCTGGGGTTGCCTCGTCTTCTGCTACTACCACGGACTTTCTGCCTGTCGCCTCTGATGCCACCTCGATTGATGTCGATGCTGTCACCGAGGCTGAGTTGAAGGAGAATGGCTTCAGGAGCACCAGGAGGACCAAGCTTATCTGTACTATTGGCCCTGCTACTTGCGGATTCGAGCAGCTTGAGGCGCTTGCTGTCGGCGGTATGAATGTTGCCAGGATCAATATGTGCCATGGGACTCGAGAGTGGCATCAGACGGTTATTGAACGCGTGCGGAGGCTCAATGAGGAGAAGGGTTATGCTGTGGCTATTATGATGGATACTGAAGGGAGTGAAATTCATATGGGTGATCTTGGTGGAGCTTCTTCGGCTAAAGCGGAAGTGAGTTCTGTTCTTTGTCAAATTTTCATCTTATTGTGTGACAACCGGTCGAATTATTGCTAATCTGTGTCCTAGATTTTTAAGAGTTTACTGGCTTTTGTCTGGCTTACAAATAGAAGGGTATAATTAAGTTGTTGATTTACAATTAGATTTCAACTTTCTTCTGCCTGAAACTCTAAACTCCACCCAATTTATAGATTCTTATATTGGACCAGTCTTCACTTTCCTTGAAACCTGGTTCTGAGACAGATATTGCCCATTTTATGTTATTTTAAAAATAAGATGGGTCCTTTCATCATTCAGCGAGACGTGAGCTCAAGGCTTTTAAATGTATACCTTTTTGTCGTAAGCTACTTGTCACGATATCTTGGCTTAAAGTATAAACCTTTATCTTTGGATTAATGAGGAGTTTTTGGTGCAGCTATAAATTCTCTTGAAGTGATTCTTATGCGTTAGCTCAGTTTTTCCAATTCTAAGTAATTATATGGGAAGATTGTCCCGAATGTCTGCTATCTTACTGATGTTTGACTTCGGTTTAAATAGACCAACAGAAGTCGTTGGGTGCGACATGTATAACTGCTTGCTCTGTTCTATGTAGGATGGTGAAATTTGGACGTTCAGTGTCAGAGCTTTTGATTCAACACTCCCAGAACGCACTATTAATGTAAACTATGAAGGGTTTGCAGAAGGTTACTGATTCTTCCCTAAATCAAAATTTCGTTCTGATATTTACTGAATTCATACACAATCTAATATAGCCTTTGGAAATGGTGAATTTGGATTGGATGTATCTGCGTTCATTATTGTAGTAATTTCCATGATGTTATTAATCTTGAATTATAATTGATATTGTGTAGATGTGAGAGTGGGTGATGAACTCCTTGTTGACGGTGGAATGGTGAGGTTTGAGGTGATCGAAAAGATTGGTCCTGATGTTAAGTGCCTGTGTACTGACCCGGGATTGTTGTTGCCACGAGCTAATTTGACTTTTTGGAGGGATGGACATCTAGTACGAGAACGTAATGCCATGCTCCCTACAATTTCTTCTAAGGTAGGCCCCCTGCTCCTTCAACTGCCATTCCCTTTACACGGAAACAATTTGTTAATTTGATTTAACATGATATTTGAGTAGGACTGTAGGAGGTTCTACAAAACCCCCTATAATGTCATTTCCTCCCCAATTAATATTGATAACCACTTTTTTGAGCCTTTTGCAAATTTCTAACCCCATTAGCGAGGAAGAGTGTTGATATAAGTAGAAAGAGTTTATGGTGACCTACAAGTTGTCGTGCATATAGTATTAAATTTACAGTAACCTATGAACTTAAGCTTTTAGTTTTAGGTTTAGAAGTGATTTAACTGTTACTATTGAGTGCCATGGTGTATAACCAATATTAGAAAGAGTTTCATACTATGACGAGTTCAATCATCCAATCTTGGTATATTTTCCTGAATCTGAGTAATGTGTATAGCTAATTCTAGATACATTGTTTTAGAAAATGTAAGCTTTGTATTATTTTAATTATGTTATGTTTCTAAGTGCCAGAAATGTTACCTAGAATTGGAACGACCATTACTGTCCAATCATAATCTAGCACTATTGTTTAATTAGCTTAGATATTCAAACTTTTGATATTGGCATTACCACAAACTCTCAAGTGTTTTCTGTTTACACTTGGTAGAGTAACTAGATTTATGTTGAAAGAATGATAGTTTGCATCATAAAATTTAGGTCATATTTAATGTGGTTCCGTAGTAGTATATCATACTGTAAACTCTTTATGCAACCTAATGACTGCATCTGTTTCTGGGTCTTCGTCTCATTATCTTTGAGGTTAACCTTTCGTGCAACCTAATGGTGAAAAATGCCCTGACACAGTGACTTATTTTCTAGTCTAATACAAGTTTAATGAATCCAGGATTGGTTGGATATTGATTTTGGGATTGCTGAAGGTGTTGATTTTCTTGCCATATCATTTGTCAAGTCAGCTGAAGTGATAAAACATCTTAAAAGCTATATTGCTGCACGTTCTCGTGGCAGGTAAGCAGAATTGTGGTAATGTTGCTGATTGGTTCTTCTTTTATTCCATTTGGATCCGAAGCTATGGACCTCTCATTGTTGATTCTCACCAAATTTTCTCTCCAATTCCGTGAATTTCATCTCTAAAATGTTTATATGTGCGCCTTCTAAATATTCATGTTTTTAAATTTGTTATGCAGTGACATTTCCATCATTGCAAAAATAGAGAGTCTGGACTCATTGAAGAACTTGGAAGAAATTATTGTAGCATCAGATGGAGCTATGGTAGCTAGAGGAGATTTAGGAGCTCAAATACCACTGGAACAGGTGCCATCAGTCCAGCAAAAGGTCGTCCAACTATGCAGGCAGTTAAATAAGCCAGTTATTGTTGCTTCTCAGCTGCTTGAGTCGATGATTGAATACCCTACACCCACCAGAGCTGAAGTTGCTGATGTTTCTGAGGCCGTTAGGCAGCGATCAGATGCTCTAATGCTCTCTGGTGAGTCCGCCATGGGCCAGTACCCCGAGAAGGCATTGGCTGTTTTGAGAAGTGTCAGTCTAAGAATTGAGAAGTGGTGGAGGGATGAGAAACTCCATGAACCTATGGAACTCCCAGAAGTTGGATCTTCATTCTCAGATAGTATCTTAGAAGAGATCTGCAATTCGGCTGCCAAAATGGGTAAGCTTTATCATATTCTCCAGCTCCAGATAATATACCTATACTATTGTTTAGATCTATGCTCTAGAACTATGAAGAACACAAATACTATCTCATATCACCATGTTTTGCTAACAATGCCAACATTGAACCACCATGAGTTGGTATATCGATCAAAAAGGGTTATTTAAATAATAAAGACCTTGGAAGGGGAATGAGTTCAAGTCATAGTGGCCACCTAACTAAAATTTAATATCCTAAGAGTTACTTTGACAGCTAAACGTAGTAGGGTCTGATGGTTGTCTTGTAGTATAATCGAGGAGGTATTCCTAAGCTAGTTTGGACACTCATGGATATTAAAAAGAATGCTAGCGTTGAAAATGGTTCGTTGAGTTGGGCCTATTCATAGGCTATGTCTTCTTTCCCTCACTTCTTTATGGTGCTGTTCCTGTTGCTTATTCCAGTCTAACTGTGAGTTTTGTTTCTCTAGCCAATAATTTGGAGGTGGATGCGATTTTCGTCTACACAAAGACAGGCCACATGGCATCTCTCCTGTCCCGATGCCGACCCGATTGCCCGATCTTTGCATTTACTTCCACGACATCTGTGAGGAGACGTCTTAACTTGCAATGGGGCTTGATACCCTTCCGCCTGAGCTTCTCGGATGACATGGAAAACAATCTCAACAAAACATTTTTGCTGCTTAAAGCCAGAAACTTGATCAAATCAGGTGACCTTGTAATTGCTGTCTCAGACATGTTGCAGTCTATCCAAGTTATGAATGTTCCATAA

mRNA sequence

ATGACACAGTCTCTGCAGCTTTTCTCCTCCTCCTCCACCCGCTCTCCGCCCATCTCTCTCCCCAAACATTCCCTCTCTAAACCCTCCCCTTCCTCCGCCGCCTTCCGCTTCCCCTTCTCCTTCACCAAATCCTCAATCAGAGCCTCTTCCTCCCCGGATCTCACTTCCCCAGTCCTCGTTTCTGCCAATGGCTCCAGTTCCGGAGCTGGGGTTGCCTCGTCTTCTGCTACTACCACGGACTTTCTGCCTGTCGCCTCTGATGCCACCTCGATTGATGTCGATGCTGTCACCGAGGCTGAGTTGAAGGAGAATGGCTTCAGGAGCACCAGGAGGACCAAGCTTATCTGTACTATTGGCCCTGCTACTTGCGGATTCGAGCAGCTTGAGGCGCTTGCTGTCGGCGGTATGAATGTTGCCAGGATCAATATGTGCCATGGGACTCGAGAGTGGCATCAGACGGTTATTGAACGCGTGCGGAGGCTCAATGAGGAGAAGGGTTATGCTGTGGCTATTATGATGGATACTGAAGGGAGTGAAATTCATATGGGTGATCTTGGTGGAGCTTCTTCGGCTAAAGCGGAAGATGGTGAAATTTGGACGTTCAGTGTCAGAGCTTTTGATTCAACACTCCCAGAACGCACTATTAATGTAAACTATGAAGGGTTTGCAGAAGATGTGAGAGTGGGTGATGAACTCCTTGTTGACGGTGGAATGGTGAGGTTTGAGGTGATCGAAAAGATTGGTCCTGATGTTAAGTGCCTGTGTACTGACCCGGGATTGTTGTTGCCACGAGCTAATTTGACTTTTTGGAGGGATGGACATCTAGTACGAGAACGTAATGCCATGCTCCCTACAATTTCTTCTAAGGATTGGTTGGATATTGATTTTGGGATTGCTGAAGGTGTTGATTTTCTTGCCATATCATTTGTCAAGTCAGCTGAAGTGATAAAACATCTTAAAAGCTATATTGCTGCACGTTCTCGTGGCAGTGACATTTCCATCATTGCAAAAATAGAGAGTCTGGACTCATTGAAGAACTTGGAAGAAATTATTGTAGCATCAGATGGAGCTATGGTAGCTAGAGGAGATTTAGGAGCTCAAATACCACTGGAACAGGTGCCATCAGTCCAGCAAAAGGTCGTCCAACTATGCAGGCAGTTAAATAAGCCAGTTATTGTTGCTTCTCAGCTGCTTGAGTCGATGATTGAATACCCTACACCCACCAGAGCTGAAGTTGCTGATGTTTCTGAGGCCGTTAGGCAGCGATCAGATGCTCTAATGCTCTCTGGTGAGTCCGCCATGGGCCAGTACCCCGAGAAGGCATTGGCTGTTTTGAGAAGTGTCAGTCTAAGAATTGAGAAGTGGTGGAGGGATGAGAAACTCCATGAACCTATGGAACTCCCAGAAGTTGGATCTTCATTCTCAGATAGTATCTTAGAAGAGATCTGCAATTCGGCTGCCAAAATGGGCTATGTCTTCTTTCCCTCACTTCTTTATGGTGCTGTTCCTGTTGCTTATTCCAGTCTAACTGTGAGTTTTGTTTCTCTAGCCAATAATTTGGAGGTGGATGCGATTTTCGTCTACACAAAGACAGGCCACATGGCATCTCTCCTGTCCCGATGCCGACCCGATTGCCCGATCTTTGCATTTACTTCCACGACATCTGTGAGGAGACGTCTTAACTTGCAATGGGGCTTGATACCCTTCCGCCTGAGCTTCTCGGATGACATGGAAAACAATCTCAACAAAACATTTTTGCTGCTTAAAGCCAGAAACTTGATCAAATCAGGTGACCTTGTAATTGCTGTCTCAGACATGTTGCAGTCTATCCAAGTTATGAATGTTCCATAA

Coding sequence (CDS)

ATGACACAGTCTCTGCAGCTTTTCTCCTCCTCCTCCACCCGCTCTCCGCCCATCTCTCTCCCCAAACATTCCCTCTCTAAACCCTCCCCTTCCTCCGCCGCCTTCCGCTTCCCCTTCTCCTTCACCAAATCCTCAATCAGAGCCTCTTCCTCCCCGGATCTCACTTCCCCAGTCCTCGTTTCTGCCAATGGCTCCAGTTCCGGAGCTGGGGTTGCCTCGTCTTCTGCTACTACCACGGACTTTCTGCCTGTCGCCTCTGATGCCACCTCGATTGATGTCGATGCTGTCACCGAGGCTGAGTTGAAGGAGAATGGCTTCAGGAGCACCAGGAGGACCAAGCTTATCTGTACTATTGGCCCTGCTACTTGCGGATTCGAGCAGCTTGAGGCGCTTGCTGTCGGCGGTATGAATGTTGCCAGGATCAATATGTGCCATGGGACTCGAGAGTGGCATCAGACGGTTATTGAACGCGTGCGGAGGCTCAATGAGGAGAAGGGTTATGCTGTGGCTATTATGATGGATACTGAAGGGAGTGAAATTCATATGGGTGATCTTGGTGGAGCTTCTTCGGCTAAAGCGGAAGATGGTGAAATTTGGACGTTCAGTGTCAGAGCTTTTGATTCAACACTCCCAGAACGCACTATTAATGTAAACTATGAAGGGTTTGCAGAAGATGTGAGAGTGGGTGATGAACTCCTTGTTGACGGTGGAATGGTGAGGTTTGAGGTGATCGAAAAGATTGGTCCTGATGTTAAGTGCCTGTGTACTGACCCGGGATTGTTGTTGCCACGAGCTAATTTGACTTTTTGGAGGGATGGACATCTAGTACGAGAACGTAATGCCATGCTCCCTACAATTTCTTCTAAGGATTGGTTGGATATTGATTTTGGGATTGCTGAAGGTGTTGATTTTCTTGCCATATCATTTGTCAAGTCAGCTGAAGTGATAAAACATCTTAAAAGCTATATTGCTGCACGTTCTCGTGGCAGTGACATTTCCATCATTGCAAAAATAGAGAGTCTGGACTCATTGAAGAACTTGGAAGAAATTATTGTAGCATCAGATGGAGCTATGGTAGCTAGAGGAGATTTAGGAGCTCAAATACCACTGGAACAGGTGCCATCAGTCCAGCAAAAGGTCGTCCAACTATGCAGGCAGTTAAATAAGCCAGTTATTGTTGCTTCTCAGCTGCTTGAGTCGATGATTGAATACCCTACACCCACCAGAGCTGAAGTTGCTGATGTTTCTGAGGCCGTTAGGCAGCGATCAGATGCTCTAATGCTCTCTGGTGAGTCCGCCATGGGCCAGTACCCCGAGAAGGCATTGGCTGTTTTGAGAAGTGTCAGTCTAAGAATTGAGAAGTGGTGGAGGGATGAGAAACTCCATGAACCTATGGAACTCCCAGAAGTTGGATCTTCATTCTCAGATAGTATCTTAGAAGAGATCTGCAATTCGGCTGCCAAAATGGGCTATGTCTTCTTTCCCTCACTTCTTTATGGTGCTGTTCCTGTTGCTTATTCCAGTCTAACTGTGAGTTTTGTTTCTCTAGCCAATAATTTGGAGGTGGATGCGATTTTCGTCTACACAAAGACAGGCCACATGGCATCTCTCCTGTCCCGATGCCGACCCGATTGCCCGATCTTTGCATTTACTTCCACGACATCTGTGAGGAGACGTCTTAACTTGCAATGGGGCTTGATACCCTTCCGCCTGAGCTTCTCGGATGACATGGAAAACAATCTCAACAAAACATTTTTGCTGCTTAAAGCCAGAAACTTGATCAAATCAGGTGACCTTGTAATTGCTGTCTCAGACATGTTGCAGTCTATCCAAGTTATGAATGTTCCATAA

Protein sequence

MTQSLQLFSSSSTRSPPISLPKHSLSKPSPSSAAFRFPFSFTKSSIRASSSPDLTSPVLVSANGSSSGAGVASSSATTTDFLPVASDATSIDVDAVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMELPEVGSSFSDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP

Homology

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BLAST of Spg023745 vs. NCBI nr
Match: XP_038874381.1 (pyruvate kinase isozyme A, chloroplastic [Benincasa hispida])

HSP 1 Score: 1048.5 bits (2710), Expect = 2.2e-302
Identity = 559/623 (89.73%), Postives = 575/623 (92.30%), Query Frame = 0


Query: 1   MTQSLQLFSSSSTRSPPISLPKHSLSKPSPSSAAFRFPFSFTKSSIRASSSPDL------ 60
           MTQSLQLF +SSTRSP ISLP+ SLSKPS SSAAFRFPFSF+KSSIRASSSPDL      
Sbjct: 1   MTQSLQLF-TSSTRSPGISLPRRSLSKPSSSSAAFRFPFSFSKSSIRASSSPDLNPLSSS 60

Query: 61  -TSPVLVSANGSSSGAGVASSSATTTDFLPVASDATSIDVDAVTEAELKENGFRSTRRTK 120
            TS VLVS NGSSSG GV SSSA T +F+ +ASD+TSIDVDAVTEAELKENGFRSTRRTK
Sbjct: 61  STSQVLVSENGSSSGTGVVSSSA-TKEFVSLASDSTSIDVDAVTEAELKENGFRSTRRTK 120

Query: 121 LICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGYAVAIMM 180
           L+CTIGPA+CGFEQLEALAVGGMNVARINMCHGTREWH+TVIE VRRLN+EKGYAVAIMM
Sbjct: 121 LVCTIGPASCGFEQLEALAVGGMNVARINMCHGTREWHRTVIEYVRRLNDEKGYAVAIMM 180

Query: 181 DTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDELL 240
           DTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD+LL
Sbjct: 181 DTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLL 240

Query: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300
           VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD
Sbjct: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300

Query: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVA 360
           IDFGIAEGVDF+AISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVA
Sbjct: 301 IDFGIAEGVDFIAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVA 360

Query: 361 SDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420
           SDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA
Sbjct: 361 SDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420

Query: 421 DVSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMELPEVGSS 480
           DVSEAVRQRSDALMLSGESAMGQYP+KALAVLRSVSLRIEKWWRDEK HEPMELPEVGSS
Sbjct: 421 DVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSS 480

Query: 481 FSDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFVYTKTGH 540
           FSD+ILEEICNSAAKM                           ANNLEVDAIFVYT +GH
Sbjct: 481 FSDTILEEICNSAAKM---------------------------ANNLEVDAIFVYTTSGH 540

Query: 541 MASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNL 600
           MASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNL
Sbjct: 541 MASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNL 594

Query: 601 IKSGDLVIAVSDMLQSIQVMNVP 617
           IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 601 IKSGDLVIAVSDMLQSIQVMNVP 594

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BLAST of Spg023745 vs. NCBI nr
Match: XP_004150564.1 (plastidial pyruvate kinase 1, chloroplastic [Cucumis sativus] >KGN61654.1 hypothetical protein Csa_006584 [Cucumis sativus])

HSP 1 Score: 1043.9 bits (2698), Expect = 5.5e-301
Identity = 555/623 (89.09%), Postives = 571/623 (91.65%), Query Frame = 0


Query: 1   MTQSLQLFSSSSTRSPPISLPKHSLSKPSPSSAAFRFPFSFTKSSIRASSSPDL------ 60
           MTQSLQLF+SS+     ISLPKHS+SKPS SSAAFRFPFSF+KSSIRASSS DL      
Sbjct: 1   MTQSLQLFTSSA-----ISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSS 60

Query: 61  -TSPVLVSANGSSSGAGVASSSATTTDFLPVASDATSIDVDAVTEAELKENGFRSTRRTK 120
            TS VLVS NGSSSG GV  SSA T +F+ VASD+TSI+VDAVTEAELKENGFRSTRRTK
Sbjct: 61  STSQVLVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTK 120

Query: 121 LICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGYAVAIMM 180
           L+CTIGPATCGFEQLEALAVGGMNVARINMCHGTR+WH+TVIERVRRLN+ KGYAVAIMM
Sbjct: 121 LVCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDGKGYAVAIMM 180

Query: 181 DTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDELL 240
           DTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD+LL
Sbjct: 181 DTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLL 240

Query: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300
           VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD
Sbjct: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300

Query: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVA 360
           IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII+A
Sbjct: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILA 360

Query: 361 SDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420
           SDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA
Sbjct: 361 SDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420

Query: 421 DVSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMELPEVGSS 480
           DVSEAVRQRSDALMLSGESAMGQYP+KALAVLRSVSLRIEKWWRDEK HEPMELPEVGSS
Sbjct: 421 DVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSS 480

Query: 481 FSDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFVYTKTGH 540
           FSDSILEEICNSAAKM                           ANNLEVDAIFVYT +GH
Sbjct: 481 FSDSILEEICNSAAKM---------------------------ANNLEVDAIFVYTTSGH 540

Query: 541 MASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNL 600
           MASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNL
Sbjct: 541 MASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNL 591

Query: 601 IKSGDLVIAVSDMLQSIQVMNVP 617
           IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 601 IKSGDLVIAVSDMLQSIQVMNVP 591

[Close]

BLAST of Spg023745 vs. NCBI nr
Match: KAG6593869.1 (hypothetical protein SDJN03_13345, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1040.4 bits (2689), Expect = 6.1e-300
Identity = 550/624 (88.14%), Postives = 567/624 (90.87%), Query Frame = 0


Query: 1   MTQSLQLFSSSSTRSPPISLPKHSLSK-PSPSSAAFRFPFSFTKSSIRASSSPDL----- 60
           M QSLQLF+SS+TRSPPISLPKHSLSK PS S+AAFR P SF K SIRASSSPDL     
Sbjct: 170 MAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 229

Query: 61  --TSPVLVSANGSSSGAGVASSSATTTDFLPVASDATSIDVDAVTEAELKENGFRSTRRT 120
             TS VLVS NGSSSG G  S+SATT +F+P  SD +SIDVD VTEAELKENGFRSTRRT
Sbjct: 230 ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 289

Query: 121 KLICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGYAVAIM 180
           KL+CTIGP TCGFEQLEALAVGGMNVARINMCHGTREWH+ VIERVRRLNEEKGYAVAIM
Sbjct: 290 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 349

Query: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDEL 240
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD+L
Sbjct: 350 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 409

Query: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 410 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 469

Query: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360
           DIDFGI+EGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV
Sbjct: 470 DIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 529

Query: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420
           ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR+LNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 530 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 589

Query: 421 ADVSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMELPEVGS 480
           ADVSEAVRQR+DALMLSGESAMGQ+PEKAL VLRSVSLRIEKWWRDEK HE MELPEVGS
Sbjct: 590 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 649

Query: 481 SFSDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFVYTKTG 540
           S+SDSILEEICNS AKM                           ANNLEVDAIFVYTKTG
Sbjct: 650 SYSDSILEEICNSTAKM---------------------------ANNLEVDAIFVYTKTG 709

Query: 541 HMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARN 600
           HMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTF+LLKARN
Sbjct: 710 HMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARN 766

Query: 601 LIKSGDLVIAVSDMLQSIQVMNVP 617
           LIKSGDLV+AVSDMLQSIQVMNVP
Sbjct: 770 LIKSGDLVLAVSDMLQSIQVMNVP 766

[Close]

BLAST of Spg023745 vs. NCBI nr
Match: KAG7026211.1 (hypothetical protein SDJN02_12710, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1040.4 bits (2689), Expect = 6.1e-300
Identity = 550/624 (88.14%), Postives = 567/624 (90.87%), Query Frame = 0


Query: 1   MTQSLQLFSSSSTRSPPISLPKHSLSK-PSPSSAAFRFPFSFTKSSIRASSSPDL----- 60
           M QSLQLF+SS+TRSPPISLPKHSLSK PS S+AAFR P SF K SIRASSSPDL     
Sbjct: 62  MAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 121

Query: 61  --TSPVLVSANGSSSGAGVASSSATTTDFLPVASDATSIDVDAVTEAELKENGFRSTRRT 120
             TS VLVS NGSSSG G  S+SATT +F+P  SD +SIDVD VTEAELKENGFRSTRRT
Sbjct: 122 ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 181

Query: 121 KLICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGYAVAIM 180
           KL+CTIGP TCGFEQLEALAVGGMNVARINMCHGTREWH+ VIERVRRLNEEKGYAVAIM
Sbjct: 182 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 241

Query: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDEL 240
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD+L
Sbjct: 242 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 301

Query: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 302 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 361

Query: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360
           DIDFGI+EGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV
Sbjct: 362 DIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 421

Query: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420
           ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR+LNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 422 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 481

Query: 421 ADVSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMELPEVGS 480
           ADVSEAVRQR+DALMLSGESAMGQ+PEKAL VLRSVSLRIEKWWRDEK HE MELPEVGS
Sbjct: 482 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 541

Query: 481 SFSDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFVYTKTG 540
           S+SDSILEEICNS AKM                           ANNLEVDAIFVYTKTG
Sbjct: 542 SYSDSILEEICNSTAKM---------------------------ANNLEVDAIFVYTKTG 601

Query: 541 HMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARN 600
           HMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTF+LLKARN
Sbjct: 602 HMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARN 658

Query: 601 LIKSGDLVIAVSDMLQSIQVMNVP 617
           LIKSGDLV+AVSDMLQSIQVMNVP
Sbjct: 662 LIKSGDLVLAVSDMLQSIQVMNVP 658

[Close]

BLAST of Spg023745 vs. NCBI nr
Match: XP_023000477.1 (pyruvate kinase isozyme A, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1038.5 bits (2684), Expect = 2.3e-299
Identity = 550/624 (88.14%), Postives = 566/624 (90.71%), Query Frame = 0


Query: 1   MTQSLQLFSSSSTRSPPISLPKHSLSK-PSPSSAAFRFPFSFTKSSIRASSSPDL----- 60
           M QSLQLF+SS+TRSP ISLPKHSLSK PS S+AAFR P SF K SIRASSSPDL     
Sbjct: 1   MAQSLQLFTSSTTRSPSISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 60

Query: 61  --TSPVLVSANGSSSGAGVASSSATTTDFLPVASDATSIDVDAVTEAELKENGFRSTRRT 120
             TS VLVS NGSSSG G  S+SATT +F+P  SD +SIDVD VTEAELKENGFRSTRRT
Sbjct: 61  ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 120

Query: 121 KLICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGYAVAIM 180
           KL+CTIGP TCGFEQLEALAVGGMNVARINMCHGTREWH+ VIERVRRLNEEKGYAVAIM
Sbjct: 121 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 180

Query: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDEL 240
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD+L
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240

Query: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360
           DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV
Sbjct: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360

Query: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420
           ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR+LNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 421 ADVSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMELPEVGS 480
           ADVSEAVRQR+DALMLSGESAMGQ+PEKAL VLRSVSLRIEKWWRDEK HE MELPEVGS
Sbjct: 421 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 480

Query: 481 SFSDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFVYTKTG 540
           S+SDSILEEICNS AKM                           ANNLEVDAIFVYTKTG
Sbjct: 481 SYSDSILEEICNSTAKM---------------------------ANNLEVDAIFVYTKTG 540

Query: 541 HMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARN 600
           HMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTF+LLKARN
Sbjct: 541 HMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARN 597

Query: 601 LIKSGDLVIAVSDMLQSIQVMNVP 617
           LIKSGDLV+AVSDMLQSIQVMNVP
Sbjct: 601 LIKSGDLVLAVSDMLQSIQVMNVP 597

[Close]

BLAST of Spg023745 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 2.6e-261
Identity = 487/620 (78.55%), Postives = 531/620 (85.65%), Query Frame = 0


Query: 1   MTQSLQLFSSSSTRSPPISLPKHSLSKPSPSSAAFRFPFSFTKS-SIRASSSPDLTS--P 60
           M+QSL    + +    P   PK  L  P+ +S   R+P +  KS SI+AS+SP  +S   
Sbjct: 1   MSQSLHFSPNLTFAKQP--FPKLPLPFPTSNS---RYPVNNYKSLSIKASTSPSSSSDPQ 60

Query: 61  VLVSANGS-SSGAGVASSSATTTDFLPVASDATSIDVDAVTEAELKENGFRSTRRTKLIC 120
           VLV+ NG+ +SG    +++ + T      SD +SI+VDAVTE ELKENGFRSTRRTKL+C
Sbjct: 61  VLVADNGTGNSGVLYNNNNKSVT-----VSDPSSIEVDAVTETELKENGFRSTRRTKLVC 120

Query: 121 TIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGYAVAIMMDTE 180
           TIGPATCGFE+LEALAVGGMNVARINMCHGTREWH++VIERVRRLNEEKG+AVAIMMDTE
Sbjct: 121 TIGPATCGFEELEALAVGGMNVARINMCHGTREWHKSVIERVRRLNEEKGFAVAIMMDTE 180

Query: 181 GSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDELLVDG 240
           GSEIHMGDLGGASSAKAEDGEIWTFSVRA+DS  PERTINVNY+GFAEDV+VGDELLVDG
Sbjct: 181 GSEIHMGDLGGASSAKAEDGEIWTFSVRAYDSPRPERTINVNYDGFAEDVKVGDELLVDG 240

Query: 241 GMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDF 300
           GMVRFEVIEKIGPDVKC CTDPGLLLPRANLTFWRDG LVRERNAMLPTISSKDWLDIDF
Sbjct: 241 GMVRFEVIEKIGPDVKCRCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSKDWLDIDF 300

Query: 301 GIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVASDG 360
           GIAEGVDF+AISFVKSAEVI HLKSYIAARSR SDI++IAKIES+DSLKNLEEII ASDG
Sbjct: 301 GIAEGVDFIAISFVKSAEVINHLKSYIAARSRDSDIAVIAKIESIDSLKNLEEIIRASDG 360

Query: 361 AMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVS 420
           AMVARGDLGAQIPLEQVPS QQ +VQ+CRQLNKPVIVASQLLESMIEYPTPTRAEVADVS
Sbjct: 361 AMVARGDLGAQIPLEQVPSAQQNIVQVCRQLNKPVIVASQLLESMIEYPTPTRAEVADVS 420

Query: 421 EAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMELPEVGSSFSD 480
           EAVRQR+DALMLSGESAMGQYPEKALAVLRSVS+RIEKWWR+EK HE MELP +GS++SD
Sbjct: 421 EAVRQRADALMLSGESAMGQYPEKALAVLRSVSVRIEKWWREEKHHEAMELPAIGSTYSD 480

Query: 481 SILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFVYTKTGHMAS 540
           SI EEICNSAAKM                           ANNL VDA+FVYTK GHMAS
Sbjct: 481 SISEEICNSAAKM---------------------------ANNLGVDALFVYTKDGHMAS 540

Query: 541 LLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKS 600
           LLSRCRPDCPIFAFT+TTSVRRRLNLQWGLIPFRLSF+DDME+NLNKTF LLKAR +IKS
Sbjct: 541 LLSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIKS 583

Query: 601 GDLVIAVSDMLQSIQVMNVP 617
           GDLVIAVSDMLQSIQVMNVP
Sbjct: 601 GDLVIAVSDMLQSIQVMNVP 583

[Close]

BLAST of Spg023745 vs. ExPASy Swiss-Prot
Match: Q40545 (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 1.6e-258
Identity = 479/629 (76.15%), Postives = 529/629 (84.10%), Query Frame = 0


Query: 1   MTQSLQLFSSSSTRSPPISLPKHSLSKPS--PSSAAFRFPFSFTKSSIR-------ASSS 60
           M+Q+L  F SSS+RSP       ++S+PS  PS+ + R      K S+R       ++++
Sbjct: 1   MSQALNFFVSSSSRSP----ATFTISRPSVFPSTGSLRL---LVKKSLRTLVVEASSAAA 60

Query: 61  PDL----TSPVLVSANGSSSGAGVASSSATTTDFLPVASDATSIDVDAVTEAELKENGFR 120
            DL    +SPVLVS NG  SG  ++S++          +D++SI+VD VTEAELKENGFR
Sbjct: 61  SDLDEPQSSPVLVSENG--SGGVLSSATQEYGRNAAPGTDSSSIEVDTVTEAELKENGFR 120

Query: 121 STRRTKLICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGY 180
           STRRTKLICTIGPATCGFEQLE LA GGMNVARINMCHGTREWH+ VIER+RRLNEEKG+
Sbjct: 121 STRRTKLICTIGPATCGFEQLERLAEGGMNVARINMCHGTREWHRMVIERLRRLNEEKGF 180

Query: 181 AVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVR 240
           AVAIMMDTEGSEIHMGDLGGASSAKAEDGEIW F+VR+FD  LPERT+ VNY+GFAEDV+
Sbjct: 181 AVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWNFTVRSFDPPLPERTVTVNYDGFAEDVK 240

Query: 241 VGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTIS 300
           VGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTIS
Sbjct: 241 VGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGKLVRERNAMLPTIS 300

Query: 301 SKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNL 360
           SKDWLDIDFGIAEGVDF+A+SFVKSAEVIKHLKSYI AR+R SDIS+IAKIES+DSLKNL
Sbjct: 301 SKDWLDIDFGIAEGVDFIAVSFVKSAEVIKHLKSYIQARARDSDISVIAKIESIDSLKNL 360

Query: 361 EEIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTP 420
           EEII ASDGAMVARGDLGAQIPLEQVPS QQK+VQ+CRQLN+PVIVASQLLESMIEYP P
Sbjct: 361 EEIIQASDGAMVARGDLGAQIPLEQVPSEQQKIVQICRQLNRPVIVASQLLESMIEYPIP 420

Query: 421 TRAEVADVSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMEL 480
           TRAEVADVSEAVRQR DALMLSGESAMGQ+PEKAL VLRSVSLRIE+ WR++K HE +EL
Sbjct: 421 TRAEVADVSEAVRQRGDALMLSGESAMGQFPEKALTVLRSVSLRIERMWREQKRHEVIEL 480

Query: 481 PEVGSSFSDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFV 540
           P + SSFSDSI EEICNSAAKM                           ANNLEVDA+FV
Sbjct: 481 PSIASSFSDSISEEICNSAAKM---------------------------ANNLEVDALFV 540

Query: 541 YTKTGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLL 600
           YTK GHMASLLSRCRPDCPIFAFT+TTSVRRRLNLQWGL+PFRLSFSDDME+NLNKTF L
Sbjct: 541 YTKNGHMASLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLMPFRLSFSDDMESNLNKTFSL 593

Query: 601 LKARNLIKSGDLVIAVSDMLQSIQVMNVP 617
           LKAR +IKSGDL+IAVSDMLQSIQVMNVP
Sbjct: 601 LKARGMIKSGDLIIAVSDMLQSIQVMNVP 593

[Close]

BLAST of Spg023745 vs. ExPASy Swiss-Prot
Match: Q9LIK0 (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 7.4e-256
Identity = 471/625 (75.36%), Postives = 522/625 (83.52%), Query Frame = 0


Query: 1   MTQSLQLFSSSSTRSPPISLPKHSLSKPSPSSAAFRFPFSFTK-SSIRASSSPDLTSPVL 60
           M+QS+Q FS+ S     + LP    ++P  S +  RFP +  K +SIRASSS    SP L
Sbjct: 1   MSQSIQ-FSTPSHTPHLLHLPHSQFNRPLSSISFRRFPLTTIKYTSIRASSSSS-PSPDL 60

Query: 61  VSANGSSS--------GAGVASSSATTTDFLPVASDATSIDVDAVTEAELKENGFRSTRR 120
            S++ SSS        G G   S   +     V +D + I+VD VTEAELKENGFRSTRR
Sbjct: 61  DSSSSSSSSQVLLSPNGTGAVKSDERSVVATAVTTDTSGIEVDTVTEAELKENGFRSTRR 120

Query: 121 TKLICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGYAVAI 180
           TKLICTIGPATCGFEQLEALAVGGMNVAR+NMCHGTR+WH+ VI  VRRLNEEKG+AVAI
Sbjct: 121 TKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEEKGFAVAI 180

Query: 181 MMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDE 240
           MMDTEGSEIHMGDLGG +SAKAEDGE+WTF+VRAFDS+ PERTI+V+Y+GFAEDVRVGDE
Sbjct: 181 MMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVRVGDE 240

Query: 241 LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDW 300
           LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTISSKDW
Sbjct: 241 LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSKDW 300

Query: 301 LDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII 360
           LDIDFGIAEGVDF+A+SFVKSAEVI HLKSY+AARSRG +I +IAKIES+DSL NLEEII
Sbjct: 301 LDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLEEII 360

Query: 361 VASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAE 420
           +ASDGAMVARGDLGAQIPLEQVP+ QQ++VQ+CR LNKPVIVASQLLESMIEYPTPTRAE
Sbjct: 361 LASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTPTRAE 420

Query: 421 VADVSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMELPEVG 480
           VADVSEAVRQRSDALMLSGESAMGQ+P+KAL VLR+VSLRIE+WWR+EK HE + L  +G
Sbjct: 421 VADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPLQAIG 480

Query: 481 SSFSDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFVYTKT 540
           SSFSD I EEICNSAAKM                           ANNL VDA+FVYT +
Sbjct: 481 SSFSDKISEEICNSAAKM---------------------------ANNLGVDAVFVYTTS 540

Query: 541 GHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKAR 600
           GHMASL+SRCRPDCPIFAFT+TTSVRRRLNLQWGLIPFRLSFSDDME+NLNKTF LLK+R
Sbjct: 541 GHMASLVSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSR 596

Query: 601 NLIKSGDLVIAVSDMLQSIQVMNVP 617
            +IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 601 GMIKSGDLVIAVSDMLQSIQVMNVP 596

[Close]

BLAST of Spg023745 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 9.7e-99
Identity = 216/504 (42.86%), Postives = 304/504 (60.32%), Query Frame = 0


Query: 108 STRRTKLICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEE-KG 167
           S R+TK++CTIGP+T   E +  LA  GMNVAR+NM HG    HQ  I+ V+  N + + 
Sbjct: 89  SPRKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFED 148

Query: 168 YAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDV 227
             +AIM+DT+G E+  GD+        ++G+ + FS++   ST  E T++VNY+ F  DV
Sbjct: 149 KVIAIMLDTKGPEVISGDV--PKPILLKEGQEFNFSIKRGVST--EDTVSVNYDDFINDV 208

Query: 228 RVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTI 287
             GD LLVDGGM+   V  K    VKC   D G L  R +L        VR ++A LP+I
Sbjct: 209 EAGDILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSRRHLN-------VRGKSATLPSI 268

Query: 288 SSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKN 347
           + KDW DI FG+   VDF A+SFVK A+V+  LK Y+  +S  +DI +I KIES DS+ N
Sbjct: 269 TEKDWDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYL--KSCNADIHVIVKIESADSIPN 328

Query: 348 LEEIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPT 407
           L  II ASDGAMVARGDLGA++P+E+VP +Q+ +++ C+ + KPVIVA+ +LESMI++PT
Sbjct: 329 LHSIISASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPT 388

Query: 408 PTRAEVADVSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPME 467
           PTRAEV+D+S AVR+ +DA+MLSGE+A G+YP KA+ V+  V+LR E       L +   
Sbjct: 389 PTRAEVSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALRTE-----SSLQKSTS 448

Query: 468 LPEVGSSFSDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIF 527
            P   +++            + MG +F           A+ S      S+AN L    I 
Sbjct: 449 SPSQSAAYK-----------SHMGEMF-----------AFHS-----SSMANTLST-PII 508

Query: 528 VYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFL 587
           V+T+TG MA +LS  RP   +FAFT+   V++RL L  G++P  + FS D E   ++   
Sbjct: 509 VFTRTGSMAIILSHNRPSSTVFAFTNNERVKQRLALYHGVVPIYMEFSSDAEETFSRAIK 546

Query: 588 LLKARNLIKSGDLVIAVSDMLQSI 611
           LL +++L+K G  V  V    Q I
Sbjct: 569 LLLSKSLVKDGQYVTLVQSGAQPI 546

[Close]

BLAST of Spg023745 vs. ExPASy Swiss-Prot
Match: Q93Z53 (Plastidial pyruvate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP3 PE=1 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 2.4e-97
Identity = 217/527 (41.18%), Postives = 316/527 (59.96%), Query Frame = 0


Query: 85  ASDATSIDVDAVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARINMC 144
           A D   +D  +   A+ + +   S R+TK++CTIGP++   E +  LA  GMNVAR+NM 
Sbjct: 74  AFDVGVLDSSSYRLADSRTSSNDSRRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMS 133

Query: 145 HGTREWHQTVIERVRRLNE-EKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSV 204
           HG    HQ  I+ V+  N      A+AIM+DT+G E+  GD+        E+G+ + F++
Sbjct: 134 HGDHASHQITIDLVKEYNSLFVDKAIAIMLDTKGPEVRSGDV--PQPIFLEEGQEFNFTI 193

Query: 205 RAFDSTLPERTINVNYEGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLP 264
           +   S   + T++VNY+ F  DV VGD LLVDGGM+   V  K    VKC+  D G L  
Sbjct: 194 KRGVSL--KDTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQS 253

Query: 265 RANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYI 324
           R +L        VR ++A LP+I+ KDW DI FG+   VDF A+SFVK A+V+  LK+Y+
Sbjct: 254 RRHLN-------VRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYL 313

Query: 325 AARSRGSDISIIAKIESLDSLKNLEEIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQL 384
             ++  +DIS+I KIES DS+KNL  II A DGAMVARGDLGA++P+E+VP +Q+++++ 
Sbjct: 314 --KTCSADISVIVKIESADSIKNLPSIISACDGAMVARGDLGAELPIEEVPLLQEEIIRR 373

Query: 385 CRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQYPEKALA 444
           CR ++KPVIVA+ +LESMI +PTPTRAEV+D++ AVR+ +DA+MLSGE+A G++P KA+ 
Sbjct: 374 CRSIHKPVIVATNMLESMINHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVN 433

Query: 445 VLRSVSLRIEKWWRDEKLHEPMELPEVGSSFSDSILEEICNSAAKMGYVFFPSLLYGAVP 504
           V+ +V+LR E             LP         +      + A  G++       G + 
Sbjct: 434 VMHTVALRTE-----------ASLP---------VRTSASRTTAYKGHM-------GQMF 493

Query: 505 VAYSSLTVSFVSLANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQ 564
             ++S+      +AN L    + V+T+TG MA LLS  RP   IFAFT+   + +RL L 
Sbjct: 494 AFHASI------MANTLS-SPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQRLALY 553

Query: 565 WGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSI 611
            G++P  + FSDD E+   ++  LL+  N++K G  V  V    Q I
Sbjct: 554 QGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPI 553

[Close]

BLAST of Spg023745 vs. ExPASy TrEMBL
Match: A0A0A0LKS3 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G215480 PE=3 SV=1)

HSP 1 Score: 1043.9 bits (2698), Expect = 2.7e-301
Identity = 555/623 (89.09%), Postives = 571/623 (91.65%), Query Frame = 0


Query: 1   MTQSLQLFSSSSTRSPPISLPKHSLSKPSPSSAAFRFPFSFTKSSIRASSSPDL------ 60
           MTQSLQLF+SS+     ISLPKHS+SKPS SSAAFRFPFSF+KSSIRASSS DL      
Sbjct: 1   MTQSLQLFTSSA-----ISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSS 60

Query: 61  -TSPVLVSANGSSSGAGVASSSATTTDFLPVASDATSIDVDAVTEAELKENGFRSTRRTK 120
            TS VLVS NGSSSG GV  SSA T +F+ VASD+TSI+VDAVTEAELKENGFRSTRRTK
Sbjct: 61  STSQVLVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTK 120

Query: 121 LICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGYAVAIMM 180
           L+CTIGPATCGFEQLEALAVGGMNVARINMCHGTR+WH+TVIERVRRLN+ KGYAVAIMM
Sbjct: 121 LVCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDGKGYAVAIMM 180

Query: 181 DTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDELL 240
           DTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD+LL
Sbjct: 181 DTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLL 240

Query: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300
           VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD
Sbjct: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300

Query: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVA 360
           IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII+A
Sbjct: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILA 360

Query: 361 SDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420
           SDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA
Sbjct: 361 SDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420

Query: 421 DVSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMELPEVGSS 480
           DVSEAVRQRSDALMLSGESAMGQYP+KALAVLRSVSLRIEKWWRDEK HEPMELPEVGSS
Sbjct: 421 DVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSS 480

Query: 481 FSDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFVYTKTGH 540
           FSDSILEEICNSAAKM                           ANNLEVDAIFVYT +GH
Sbjct: 481 FSDSILEEICNSAAKM---------------------------ANNLEVDAIFVYTTSGH 540

Query: 541 MASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNL 600
           MASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNL
Sbjct: 541 MASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNL 591

Query: 601 IKSGDLVIAVSDMLQSIQVMNVP 617
           IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 601 IKSGDLVIAVSDMLQSIQVMNVP 591

[Close]

BLAST of Spg023745 vs. ExPASy TrEMBL
Match: A0A6J1KDQ9 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111494735 PE=3 SV=1)

HSP 1 Score: 1038.5 bits (2684), Expect = 1.1e-299
Identity = 550/624 (88.14%), Postives = 566/624 (90.71%), Query Frame = 0


Query: 1   MTQSLQLFSSSSTRSPPISLPKHSLSK-PSPSSAAFRFPFSFTKSSIRASSSPDL----- 60
           M QSLQLF+SS+TRSP ISLPKHSLSK PS S+AAFR P SF K SIRASSSPDL     
Sbjct: 1   MAQSLQLFTSSTTRSPSISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 60

Query: 61  --TSPVLVSANGSSSGAGVASSSATTTDFLPVASDATSIDVDAVTEAELKENGFRSTRRT 120
             TS VLVS NGSSSG G  S+SATT +F+P  SD +SIDVD VTEAELKENGFRSTRRT
Sbjct: 61  ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 120

Query: 121 KLICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGYAVAIM 180
           KL+CTIGP TCGFEQLEALAVGGMNVARINMCHGTREWH+ VIERVRRLNEEKGYAVAIM
Sbjct: 121 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 180

Query: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDEL 240
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD+L
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240

Query: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360
           DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV
Sbjct: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360

Query: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420
           ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR+LNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 421 ADVSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMELPEVGS 480
           ADVSEAVRQR+DALMLSGESAMGQ+PEKAL VLRSVSLRIEKWWRDEK HE MELPEVGS
Sbjct: 421 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 480

Query: 481 SFSDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFVYTKTG 540
           S+SDSILEEICNS AKM                           ANNLEVDAIFVYTKTG
Sbjct: 481 SYSDSILEEICNSTAKM---------------------------ANNLEVDAIFVYTKTG 540

Query: 541 HMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARN 600
           HMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTF+LLKARN
Sbjct: 541 HMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARN 597

Query: 601 LIKSGDLVIAVSDMLQSIQVMNVP 617
           LIKSGDLV+AVSDMLQSIQVMNVP
Sbjct: 601 LIKSGDLVLAVSDMLQSIQVMNVP 597

[Close]

BLAST of Spg023745 vs. ExPASy TrEMBL
Match: A0A5A7UTK2 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001510 PE=3 SV=1)

HSP 1 Score: 1036.9 bits (2680), Expect = 3.3e-299
Identity = 553/622 (88.91%), Postives = 570/622 (91.64%), Query Frame = 0


Query: 1   MTQSLQLFSSSSTRSPPISLPKHSLSKPSPSSAAFRFPFSFTKSSIRASS------SPDL 60
           MTQSLQLF+SS+     ISLPKHS+SKP  S+AAFRFPFSF+KSSIRASS      S   
Sbjct: 1   MTQSLQLFTSSA-----ISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSSDLNPLSSSS 60

Query: 61  TSPVLVSANGSSSGAGVASSSATTTDFLPVASDATSIDVDAVTEAELKENGFRSTRRTKL 120
           TS VLVS NGSSSG GV SSSA T +F+ VASD+TSIDVDAVTEAELKENGFRSTRRTKL
Sbjct: 61  TSQVLVSENGSSSGGGVVSSSA-TREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKL 120

Query: 121 ICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGYAVAIMMD 180
           +CTIGPA+CGFEQLEALAVGGMNVARINMCHGTR+WH+TVIERVRRLN+EKGYAVAIMMD
Sbjct: 121 VCTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMD 180

Query: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDELLV 240
           TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD+LLV
Sbjct: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLV 240

Query: 241 DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI 300
           DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI
Sbjct: 241 DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI 300

Query: 301 DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVAS 360
           DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII+AS
Sbjct: 301 DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILAS 360

Query: 361 DGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD 420
           DGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD
Sbjct: 361 DGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD 420

Query: 421 VSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMELPEVGSSF 480
           VSEAVRQRSDALMLSGESAMGQYP+KALAVLRSVSLRIEKWWRDEK HEPMELPEVGSSF
Sbjct: 421 VSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSF 480

Query: 481 SDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFVYTKTGHM 540
           SDSILEEICNSAAKM                           ANNLEVDAIFVYT +GHM
Sbjct: 481 SDSILEEICNSAAKM---------------------------ANNLEVDAIFVYTTSGHM 540

Query: 541 ASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLI 600
           ASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLI
Sbjct: 541 ASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLI 589

Query: 601 KSGDLVIAVSDMLQSIQVMNVP 617
           KSGDLVIAVSDMLQSIQVMNVP
Sbjct: 601 KSGDLVIAVSDMLQSIQVMNVP 589

[Close]

BLAST of Spg023745 vs. ExPASy TrEMBL
Match: A0A1S3BLQ9 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103491219 PE=3 SV=1)

HSP 1 Score: 1036.9 bits (2680), Expect = 3.3e-299
Identity = 553/622 (88.91%), Postives = 570/622 (91.64%), Query Frame = 0


Query: 1   MTQSLQLFSSSSTRSPPISLPKHSLSKPSPSSAAFRFPFSFTKSSIRASS------SPDL 60
           MTQSLQLF+SS+     ISLPKHS+SKP  S+AAFRFPFSF+KSSIRASS      S   
Sbjct: 1   MTQSLQLFTSSA-----ISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSSDLNPLSSSS 60

Query: 61  TSPVLVSANGSSSGAGVASSSATTTDFLPVASDATSIDVDAVTEAELKENGFRSTRRTKL 120
           TS VLVS NGSSSG GV SSSA T +F+ VASD+TSIDVDAVTEAELKENGFRSTRRTKL
Sbjct: 61  TSQVLVSENGSSSGGGVVSSSA-TREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKL 120

Query: 121 ICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGYAVAIMMD 180
           +CTIGPA+CGFEQLEALAVGGMNVARINMCHGTR+WH+TVIERVRRLN+EKGYAVAIMMD
Sbjct: 121 VCTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMD 180

Query: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDELLV 240
           TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD+LLV
Sbjct: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLV 240

Query: 241 DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI 300
           DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI
Sbjct: 241 DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI 300

Query: 301 DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVAS 360
           DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII+AS
Sbjct: 301 DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILAS 360

Query: 361 DGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD 420
           DGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD
Sbjct: 361 DGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD 420

Query: 421 VSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMELPEVGSSF 480
           VSEAVRQRSDALMLSGESAMGQYP+KALAVLRSVSLRIEKWWRDEK HEPMELPEVGSSF
Sbjct: 421 VSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSF 480

Query: 481 SDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFVYTKTGHM 540
           SDSILEEICNSAAKM                           ANNLEVDAIFVYT +GHM
Sbjct: 481 SDSILEEICNSAAKM---------------------------ANNLEVDAIFVYTTSGHM 540

Query: 541 ASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLI 600
           ASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLI
Sbjct: 541 ASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLI 589

Query: 601 KSGDLVIAVSDMLQSIQVMNVP 617
           KSGDLVIAVSDMLQSIQVMNVP
Sbjct: 601 KSGDLVIAVSDMLQSIQVMNVP 589

[Close]

BLAST of Spg023745 vs. ExPASy TrEMBL
Match: A0A6J1EQW0 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111436871 PE=3 SV=1)

HSP 1 Score: 1034.2 bits (2673), Expect = 2.1e-298
Identity = 547/624 (87.66%), Postives = 565/624 (90.54%), Query Frame = 0


Query: 1   MTQSLQLFSSSSTRSPPISLPKHSLSK-PSPSSAAFRFPFSFTKSSIRASSSPDL----- 60
           M QSLQLF+SS+TRSPPISL KHSLSK PS S+A FR P SF + SIRASSSPDL     
Sbjct: 1   MAQSLQLFTSSTTRSPPISLSKHSLSKPPSSSTATFRLPVSFNQFSIRASSSPDLNPLSS 60

Query: 61  --TSPVLVSANGSSSGAGVASSSATTTDFLPVASDATSIDVDAVTEAELKENGFRSTRRT 120
             TS VLVS NGSSSG G  S+SATT +F+P  SD +SIDVD VTEAELKENGFRSTRRT
Sbjct: 61  ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 120

Query: 121 KLICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGYAVAIM 180
           KL+CTIGP TCGFEQLEALAVGGMNVARINMCHGTREWH+ VIERVRRLNEEKGYAVAIM
Sbjct: 121 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 180

Query: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDEL 240
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD+L
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240

Query: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360
           DIDFGI+EGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV
Sbjct: 301 DIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360

Query: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420
           ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR+LNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 421 ADVSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMELPEVGS 480
           ADVSEAVRQR+DALMLSGESAMGQ+PEKAL VLRSVSLRIEKWWRDEK HE MELPEVGS
Sbjct: 421 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 480

Query: 481 SFSDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFVYTKTG 540
           S+SDSILEEICNS AKM                           ANNLEVDAIFVYTKTG
Sbjct: 481 SYSDSILEEICNSTAKM---------------------------ANNLEVDAIFVYTKTG 540

Query: 541 HMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARN 600
           HMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTF+LLKARN
Sbjct: 541 HMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARN 597

Query: 601 LIKSGDLVIAVSDMLQSIQVMNVP 617
           LIKSGDLV+AVSDMLQSIQVMNVP
Sbjct: 601 LIKSGDLVLAVSDMLQSIQVMNVP 597

[Close]

BLAST of Spg023745 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 884.4 bits (2284), Expect = 5.2e-257
Identity = 471/625 (75.36%), Postives = 522/625 (83.52%), Query Frame = 0


Query: 1   MTQSLQLFSSSSTRSPPISLPKHSLSKPSPSSAAFRFPFSFTK-SSIRASSSPDLTSPVL 60
           M+QS+Q FS+ S     + LP    ++P  S +  RFP +  K +SIRASSS    SP L
Sbjct: 1   MSQSIQ-FSTPSHTPHLLHLPHSQFNRPLSSISFRRFPLTTIKYTSIRASSSSS-PSPDL 60

Query: 61  VSANGSSS--------GAGVASSSATTTDFLPVASDATSIDVDAVTEAELKENGFRSTRR 120
            S++ SSS        G G   S   +     V +D + I+VD VTEAELKENGFRSTRR
Sbjct: 61  DSSSSSSSSQVLLSPNGTGAVKSDERSVVATAVTTDTSGIEVDTVTEAELKENGFRSTRR 120

Query: 121 TKLICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGYAVAI 180
           TKLICTIGPATCGFEQLEALAVGGMNVAR+NMCHGTR+WH+ VI  VRRLNEEKG+AVAI
Sbjct: 121 TKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEEKGFAVAI 180

Query: 181 MMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDE 240
           MMDTEGSEIHMGDLGG +SAKAEDGE+WTF+VRAFDS+ PERTI+V+Y+GFAEDVRVGDE
Sbjct: 181 MMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVRVGDE 240

Query: 241 LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDW 300
           LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTISSKDW
Sbjct: 241 LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSKDW 300

Query: 301 LDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII 360
           LDIDFGIAEGVDF+A+SFVKSAEVI HLKSY+AARSRG +I +IAKIES+DSL NLEEII
Sbjct: 301 LDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLEEII 360

Query: 361 VASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAE 420
           +ASDGAMVARGDLGAQIPLEQVP+ QQ++VQ+CR LNKPVIVASQLLESMIEYPTPTRAE
Sbjct: 361 LASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTPTRAE 420

Query: 421 VADVSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMELPEVG 480
           VADVSEAVRQRSDALMLSGESAMGQ+P+KAL VLR+VSLRIE+WWR+EK HE + L  +G
Sbjct: 421 VADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPLQAIG 480

Query: 481 SSFSDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFVYTKT 540
           SSFSD I EEICNSAAKM                           ANNL VDA+FVYT +
Sbjct: 481 SSFSDKISEEICNSAAKM---------------------------ANNLGVDAVFVYTTS 540

Query: 541 GHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKAR 600
           GHMASL+SRCRPDCPIFAFT+TTSVRRRLNLQWGLIPFRLSFSDDME+NLNKTF LLK+R
Sbjct: 541 GHMASLVSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSR 596

Query: 601 NLIKSGDLVIAVSDMLQSIQVMNVP 617
            +IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 601 GMIKSGDLVIAVSDMLQSIQVMNVP 596

[Close]

BLAST of Spg023745 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 357.8 bits (917), Expect = 1.7e-98
Identity = 217/527 (41.18%), Postives = 316/527 (59.96%), Query Frame = 0


Query: 85  ASDATSIDVDAVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARINMC 144
           A D   +D  +   A+ + +   S R+TK++CTIGP++   E +  LA  GMNVAR+NM 
Sbjct: 74  AFDVGVLDSSSYRLADSRTSSNDSRRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMS 133

Query: 145 HGTREWHQTVIERVRRLNE-EKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSV 204
           HG    HQ  I+ V+  N      A+AIM+DT+G E+  GD+        E+G+ + F++
Sbjct: 134 HGDHASHQITIDLVKEYNSLFVDKAIAIMLDTKGPEVRSGDV--PQPIFLEEGQEFNFTI 193

Query: 205 RAFDSTLPERTINVNYEGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLP 264
           +   S   + T++VNY+ F  DV VGD LLVDGGM+   V  K    VKC+  D G L  
Sbjct: 194 KRGVSL--KDTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQS 253

Query: 265 RANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYI 324
           R +L        VR ++A LP+I+ KDW DI FG+   VDF A+SFVK A+V+  LK+Y+
Sbjct: 254 RRHLN-------VRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYL 313

Query: 325 AARSRGSDISIIAKIESLDSLKNLEEIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQL 384
             ++  +DIS+I KIES DS+KNL  II A DGAMVARGDLGA++P+E+VP +Q+++++ 
Sbjct: 314 --KTCSADISVIVKIESADSIKNLPSIISACDGAMVARGDLGAELPIEEVPLLQEEIIRR 373

Query: 385 CRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQYPEKALA 444
           CR ++KPVIVA+ +LESMI +PTPTRAEV+D++ AVR+ +DA+MLSGE+A G++P KA+ 
Sbjct: 374 CRSIHKPVIVATNMLESMINHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVN 433

Query: 445 VLRSVSLRIEKWWRDEKLHEPMELPEVGSSFSDSILEEICNSAAKMGYVFFPSLLYGAVP 504
           V+ +V+LR E             LP         +      + A  G++       G + 
Sbjct: 434 VMHTVALRTE-----------ASLP---------VRTSASRTTAYKGHM-------GQMF 493

Query: 505 VAYSSLTVSFVSLANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQ 564
             ++S+      +AN L    + V+T+TG MA LLS  RP   IFAFT+   + +RL L 
Sbjct: 494 AFHASI------MANTLS-SPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQRLALY 553

Query: 565 WGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSI 611
            G++P  + FSDD E+   ++  LL+  N++K G  V  V    Q I
Sbjct: 554 QGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPI 553

[Close]

BLAST of Spg023745 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 354.8 bits (909), Expect = 1.4e-97
Identity = 207/502 (41.24%), Postives = 294/502 (58.57%), Query Frame = 0


Query: 110 RRTKLICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLN-EEKGYA 169
           R+TK++CT+GP+T   E +  LA  GMNVAR+NM HG    H+ VI+ V+  N + K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 170 VAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRV 229
           +AIM+DT+G E+  GDL        + G+ +TF++    ST     ++VNY+ F  DV  
Sbjct: 170 IAIMLDTKGPEVRSGDL--PQPIMLDPGQEFTFTIERGVST--PSCVSVNYDDFVNDVEA 229

Query: 230 GDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISS 289
           GD LLVDGGM+ F V  K    VKC   D G L  R +L        VR ++A LP+I+ 
Sbjct: 230 GDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLN-------VRGKSATLPSITE 289

Query: 290 KDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLE 349
           KDW DI FG+   VDF A+SFVK A+V+  LK Y+  ++ G+DI +I KIES DS+ NL 
Sbjct: 290 KDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYL--QNSGADIHVIVKIESADSIPNLH 349

Query: 350 EIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPT 409
            II ASDGAMVARGDLGA++P+E+VP +Q++++ LCR + K VIVA+ +LESMI +PTPT
Sbjct: 350 SIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPT 409

Query: 410 RAEVADVSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMELP 469
           RAEV+D++ AVR+ +DA+MLSGE+A G++P KA  V+ +V+LR E      ++      P
Sbjct: 410 RAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGEMP-----P 469

Query: 470 EVGSSFSDSILEEICNSAAKMGYVFFPSLLYGAVPVAYSSLTVSFVSLANNLEVDAIFVY 529
            +G +F + + E     A  M                           +N L    + V+
Sbjct: 470 NLGQAFKNHMSEMFAYHATMM---------------------------SNTLGTSTV-VF 529

Query: 530 TKTGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLL 589
           T+TG MA LLS  RP   I+AFT+   +++RL L  G+ P  + F+DD E         L
Sbjct: 530 TRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANALATL 565

Query: 590 KARNLIKSGDLVIAVSDMLQSI 611
             + ++K G+ +  V    Q I
Sbjct: 590 LKQGMVKKGEEIAIVQSGTQPI 565

[Close]

BLAST of Spg023745 vs. TAIR 10
Match: AT4G26390.1 (Pyruvate kinase family protein )

HSP 1 Score: 232.3 bits (591), Expect = 1.1e-60
Identity = 157/516 (30.43%), Postives = 260/516 (50.39%), Query Frame = 0


Query: 111 RTKLICTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHQTVIERVRRLNEEKGYAVA 170
           +TK++CT+GPA+     +E L + GM+VAR N  HG+ E+HQ  ++ +R+     G   A
Sbjct: 8   KTKIVCTLGPASRSVPMVEKLLMAGMSVARFNFSHGSYEYHQETLDNLRQAMLNTGMLCA 67

Query: 171 IMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD 230
           +M+DT+G EI  G L      + + G+  T S   +D    E+TI ++Y+  A+DV  G 
Sbjct: 68  VMLDTKGPEIRTGFLKDGKPIQLKQGQEITIST-DYDLKGDEKTICMSYKKLAQDVNPGM 127

Query: 231 ELLVDGGMVRFEVI--EKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISS 290
            +L   G +  +V+  +K    V+C C +  +L  R N+     G +V      LPT++ 
Sbjct: 128 VILCADGTISLKVLSCDKEKGTVRCRCENTSMLGERKNVNL--PGVVVD-----LPTLTE 187

Query: 291 KDWLDI-DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNL 350
           KD  DI ++G+   +D +A+SFV+    +  ++  +   ++   I +++K+E+ + + N 
Sbjct: 188 KDKQDILEWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAK--TILLMSKVENQEGVANF 247

Query: 351 EEIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTP 410
           ++I++ SD  M+ARGDLG +IP+E++   Q+ ++  C  + KPV+ A+Q+LESMI+ P P
Sbjct: 248 DDILINSDAFMIARGDLGMEIPIEKIFLAQKVMIYKCNFMGKPVVTATQMLESMIKSPRP 307

Query: 411 TRAEVADVSEAVRQRSDALMLSGESAMGQYPEKALAVLRSVSLRIEKWWRDEKLHEPMEL 470
           TRAE  DV+ AV   +D +MLSGE+A G YPE A+  +  + +  E              
Sbjct: 308 TRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAE-------------- 367

Query: 471 PEVGSSFSDSILEEICNSAAKMGYVFFPSLLYGAVPVA-YSSLTVSFVSLANNLEVDAIF 530
                            S    G +F   +L+ AVP++   SL  S V  A +     + 
Sbjct: 368 -----------------STLDYGDIFKRIMLHAAVPMSPMESLASSAVRTATSSRATLMM 427

Query: 531 VYTKTGHMASLLSRCRPDCPIFAFT-------------STTSVRRRLNLQWGLIPF---- 590
           V T+ G  A L+++ RP  PI +               S  +  R   +  GL+P     
Sbjct: 428 VLTRGGSTARLVAKYRPGIPILSVVVPEITSDSFDWACSNEAPARHSLIYRGLVPVLYAG 482

Query: 591 --RLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAV 604
             R S  +  E  L       K + L K+GD V+A+
Sbjct: 488 SARASIDESTEETLEFASEYGKKKQLCKTGDSVVAL 482

[Close]

BLAST of Spg023745 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 230.7 bits (587), Expect = 3.1e-60
Identity = 163/538 (30.30%), Postives = 270/538 (50.19%), Query Frame = 0


Query: 89  TSIDVDAVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARINMCHGTR 148
           ++ID++ + + EL  +G   T +TK++CT+GPA+     +E L   GMNVAR N  HG+ 
Sbjct: 2   SNIDIEGILK-ELPNDG--RTPKTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSH 61

Query: 149 EWHQTVIERVRRLNEEKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDS 208
           E+HQ  ++ +R   +  G   A+M+DT+G EI  G L   +  + ++G+  T +   +D 
Sbjct: 62  EYHQETLDNLRTAMQNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITT-DYDI 121

Query: 209 TLPERTINVNYEGFAEDVRVGDELLVDGGMVRFEVI--EKIGPDVKCLCTDPGLLLPRAN 268
              E+TI+++Y+    DV+ G+ +L   G +   V+  +     V C C +  +L  R N
Sbjct: 122 KGDEKTISMSYKKLPVDVKPGNTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKN 181

Query: 269 LTFWRDGHLVRERNAMLPTISSKDWLDI-DFGIAEGVDFLAISFVKSAEVIKHLKSYIAA 328
           +     G +V      LPT++ KD  DI  +G+   +D +A+SFV+    + +++  + +
Sbjct: 182 VNL--PGVVVD-----LPTLTDKDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGS 241

Query: 329 RSRGSDISIIAKIESLDSLKNLEEIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR 388
            S+   I +++K+E+ + + N +EI+  +D  MVARGDLG +IP+E++   Q+ ++  C 
Sbjct: 242 HSK--SIMLMSKVENQEGVLNFDEILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCN 301

Query: 389 QLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQYPEKALAVL 448
              KPV+ A+Q+LESMI+ P PTRAE  DV+ AV   +D +MLSGESA G YPE A+  +
Sbjct: 302 LAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKTM 361

Query: 449 RSVSLRIEKWWRDEKLHEPMELPEVGSSFSDSILEEICNSAAKMGYVFFPSLLYGAVPVA 508
             + +  E                               S+     +F   +    +P++
Sbjct: 362 AKICIEAE-------------------------------SSLDYNTIFKEMIRATPLPMS 421

Query: 509 -YSSLTVSFVSLANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFA-----FTSTT----- 568
              SL  S V  AN  +   I V T+ G  A L+++ RP  PI +     FTS T     
Sbjct: 422 TLESLASSAVRTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPILSVVVPVFTSDTFNWSC 481

Query: 569 ---SVRRRLNLQWGLIPF------RLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAV 604
              S  R   +  GLIP       + + S+  E  +         + L   GD V+A+
Sbjct: 482 SDESPARHSLIYRGLIPVLGEGSAKATDSESTEEIIESALKSATEKGLCNHGDAVVAL 495

The following BLAST results are available for this feature:

BLAST of Spg023745 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038874381.1	2.2e-302	89.73	pyruvate kinase isozyme A, chloroplastic [Benincasa hispida]	[more]
XP_004150564.1	5.5e-301	89.09	plastidial pyruvate kinase 1, chloroplastic [Cucumis sativus] >KGN61654.1 hypoth...	[more]
KAG6593869.1	6.1e-300	88.14	hypothetical protein SDJN03_13345, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7026211.1	6.1e-300	88.14	hypothetical protein SDJN02_12710, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023000477.1	2.3e-299	88.14	pyruvate kinase isozyme A, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of Spg023745 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43117	2.6e-261	78.55	Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1	[more]
Q40545	1.6e-258	76.15	Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q9LIK0	7.4e-256	75.36	Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P...	[more]
Q40546	9.7e-99	42.86	Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q93Z53	2.4e-97	41.18	Plastidial pyruvate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P...	[more]

BLAST of Spg023745 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKS3	2.7e-301	89.09	Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G215480 PE=3 SV=1	[more]
A0A6J1KDQ9	1.1e-299	88.14	Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111494735 PE=3 SV=1	[more]
A0A5A7UTK2	3.3e-299	88.91	Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G0015...	[more]
A0A1S3BLQ9	3.3e-299	88.91	Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103491219 PE=3 SV=1	[more]
A0A6J1EQW0	2.1e-298	87.66	Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111436871 PE=3 SV=1	[more]

BLAST of Spg023745 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G22960.1	5.2e-257	75.36	Pyruvate kinase family protein	[more]
AT1G32440.1	1.7e-98	41.18	plastidial pyruvate kinase 3	[more]
AT5G52920.1	1.4e-97	41.24	plastidic pyruvate kinase beta subunit 1	[more]
AT4G26390.1	1.1e-60	30.43	Pyruvate kinase family protein	[more]
AT5G63680.1	3.1e-60	30.30	Pyruvate kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001697	Pyruvate kinase	PRINTS	PR01050	PYRUVTKNASE	coord: 334..360 score: 51.79 coord: 361..385 score: 45.91 coord: 167..183 score: 45.07 coord: 302..316 score: 41.74 coord: 411..429 score: 51.34 coord: 430..446 score: 42.17 coord: 386..410 score: 60.31
IPR001697	Pyruvate kinase	TIGRFAM	TIGR01064	TIGR01064	coord: 110..605 e-value: 1.1E-122 score: 408.0
IPR001697	Pyruvate kinase	PANTHER	PTHR11817	PYRUVATE KINASE	coord: 511..616 coord: 77..489
IPR015806	Pyruvate kinase, insert domain superfamily	GENE3D	2.40.33.10		coord: 179..283 e-value: 1.9E-114 score: 383.9
IPR040442	Pyruvate kinase-like domain superfamily	GENE3D	3.20.20.60		coord: 112..448 e-value: 1.9E-114 score: 383.9
IPR036918	Pyruvate kinase, C-terminal domain superfamily	GENE3D	3.40.1380.20		coord: 497..616 e-value: 3.3E-30 score: 106.5
IPR036918	Pyruvate kinase, C-terminal domain superfamily	SUPERFAMILY	52935	PK C-terminal domain-like	coord: 507..614
IPR015793	Pyruvate kinase, barrel	PFAM	PF00224	PK	coord: 110..455 e-value: 1.3E-92 score: 310.4
IPR015795	Pyruvate kinase, C-terminal	PFAM	PF02887	PK_C	coord: 512..606 e-value: 1.5E-20 score: 73.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..31
None	No IPR available	PANTHER	PTHR11817:SF14	PLASTIDIAL PYRUVATE KINASE 1, CHLOROPLASTIC	coord: 77..489
None	No IPR available	PANTHER	PTHR11817:SF14	PLASTIDIAL PYRUVATE KINASE 1, CHLOROPLASTIC	coord: 511..616
IPR018209	Pyruvate kinase, active site	PROSITE	PS00110	PYRUVATE_KINASE	coord: 332..344
IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily	SUPERFAMILY	51621	Phosphoenolpyruvate/pyruvate domain	coord: 96..456
IPR011037	Pyruvate kinase-like, insert domain superfamily	SUPERFAMILY	50800	PK beta-barrel domain-like	coord: 179..270

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg023745.1	Spg023745.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006096	glycolytic process
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0010431	seed maturation
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0030955	potassium ion binding
molecular_function	GO:0004743	pyruvate kinase activity
molecular_function	GO:0003824	catalytic activity