Spg023450 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg023450
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptioncytochrome P450 86A8
Locationscaffold13: 10104949 .. 10106598 (-)
RNA-Seq ExpressionSpg023450
SyntenySpg023450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGTGGGATTGGCGTTGGTTATTCTAACGGCCGTAACGGCGTATCTGCTGTGGTTCACCTTCATTTCACGGTCCTTAAAAGGCCCTCAAATGTGGCCCTTACTCGGCAGCCTCCCCGGACTCATCGAGAACTGCGACCGTTTACACGACTGGATCTCCGACAACCTCCGCGCCTGCGGCGGCACCTACCAGACCTGCATCTGGGCCTTCCCTTTCTTCGCCACCAAACAGGGCCTCGTCACCGTCACCTGCGACCCCAGAAATCTCGAGCACATTCTCAAGACCCGTTTCGACAATTACCCCAAGGGACCCACTTGGCAAGCCGTTTTTCACGACCTCCTCGGCCAGGGCATCTTTAACTCCGACGGTGACACCTGGCTCTTCCAGAGAAAAACTGCCGCCTTGGAATTTACCACTAGGACCCTCCGCCAAGCCATGGCTCGCTGGGTCAGCCGAGCCATTAAGCTTAGATTTTGCCCTATTCTCAAACAAGCCGAGCGAAATTCTAACCCCATTGATCTTCAGGATGTCTTGCTACGCCTTACTTTTGATAACATCTGCGGCTTGGCTTTCGGTAAGGACCCCATGACTTGCGCGCCCGGTTTCCCCGAGAACGGCTTTGCCACGGCTTTCGACCGAGCCACCGAGGCTTCCTTGCAACGCTTCATTTTGCCCGAAGTGCTTTGGAAATTCAAGAAGTGGCTCCGGCTTGGAATGGAGGTCAGCTTGAGCCGGAGCCTCGTCCATATTGAGGAGTACTTATCCAGTGTGATCGACTCACGTAAGCTCGAGTTGATGAATCAGCCTAAAGACTCCACCCCACACGACGATTTATTGTCGCGTTTCATGAAGAAGAAGGAAAGTAACTACTCCAACGCCTTCCTCCGCCACGTGGCGCTTAATTTCATCCTAGCTGGACGGGACACGTCGTCTGTCGCGCTGAGCTGGTTTTTCTGGTTGGTGACATTAAACCCACTCGTAGAGGAAAAAATCCTCCGCGAGATCTGCACCGTTCTGATTGAGACACGTGGCAATAACATCCCTTCATGGCTTGACGAGCCGTTAGGGTTCGAAGAGATCGACCGTCTGATTTATCTCAAAGCCGCTCTCACCGAAACCCTAAGACTGTACCCGTCGGTGCCGGAGGATTCAAAGCACGTCGTCGCCGACGACGTTCTGCCGGACGGTACCTTCGTTCCGGCGGGATCTTCGGTGACTTATTCGATCTACTCCACCGGAAGAATGAAGTCGACTTGGGGAGAGGACTGCCTCGAATTCCGGCCGGAGCGGTGGCTGACGGCGGATGGTAAGAAATTCATACCGTACGATTCGTACAAATTCGTAGCGTTCAATGCAGGGCCGAGGATCTGTTTGGGGAAGGATTTGGCGTATTTACAGATGAAGTCGATCGCAGCGGCGGTGCTGCTCCGGCACCGGCTGACGGTGGCGGCGGGGCATAAGGTGGAGCAGAAAATGTCGTTGACGCTGTTTATGAAGTACGGGCTGAAGGTGAACGTTCACCGGAGGGACTTGGAGGGGATTGTCGAAAGTATCAGGGGCAAAAACGGAATTTCGCAAGGGATAGATGCTCCGTTAATCGCCGTTGTGAACTGTAAAGGCGGTTGTAACGGAGTTAACGGAGTATAA

mRNA sequence

ATGGACGTGGGATTGGCGTTGGTTATTCTAACGGCCGTAACGGCGTATCTGCTGTGGTTCACCTTCATTTCACGGTCCTTAAAAGGCCCTCAAATGTGGCCCTTACTCGGCAGCCTCCCCGGACTCATCGAGAACTGCGACCGTTTACACGACTGGATCTCCGACAACCTCCGCGCCTGCGGCGGCACCTACCAGACCTGCATCTGGGCCTTCCCTTTCTTCGCCACCAAACAGGGCCTCGTCACCGTCACCTGCGACCCCAGAAATCTCGAGCACATTCTCAAGACCCGTTTCGACAATTACCCCAAGGGACCCACTTGGCAAGCCGTTTTTCACGACCTCCTCGGCCAGGGCATCTTTAACTCCGACGGTGACACCTGGCTCTTCCAGAGAAAAACTGCCGCCTTGGAATTTACCACTAGGACCCTCCGCCAAGCCATGGCTCGCTGGGTCAGCCGAGCCATTAAGCTTAGATTTTGCCCTATTCTCAAACAAGCCGAGCGAAATTCTAACCCCATTGATCTTCAGGATGTCTTGCTACGCCTTACTTTTGATAACATCTGCGGCTTGGCTTTCGGTAAGGACCCCATGACTTGCGCGCCCGGTTTCCCCGAGAACGGCTTTGCCACGGCTTTCGACCGAGCCACCGAGGCTTCCTTGCAACGCTTCATTTTGCCCGAAGTGCTTTGGAAATTCAAGAAGTGGCTCCGGCTTGGAATGGAGGTCAGCTTGAGCCGGAGCCTCGTCCATATTGAGGAGTACTTATCCAGTGTGATCGACTCACGTAAGCTCGAGTTGATGAATCAGCCTAAAGACTCCACCCCACACGACGATTTATTGTCGCGTTTCATGAAGAAGAAGGAAAGTAACTACTCCAACGCCTTCCTCCGCCACGTGGCGCTTAATTTCATCCTAGCTGGACGGGACACGTCGTCTGTCGCGCTGAGCTGGTTTTTCTGGTTGGTGACATTAAACCCACTCGTAGAGGAAAAAATCCTCCGCGAGATCTGCACCGTTCTGATTGAGACACGTGGCAATAACATCCCTTCATGGCTTGACGAGCCGTTAGGGTTCGAAGAGATCGACCGTCTGATTTATCTCAAAGCCGCTCTCACCGAAACCCTAAGACTGTACCCGTCGGTGCCGGAGGATTCAAAGCACGTCGTCGCCGACGACGTTCTGCCGGACGGTACCTTCGTTCCGGCGGGATCTTCGGTGACTTATTCGATCTACTCCACCGGAAGAATGAAGTCGACTTGGGGAGAGGACTGCCTCGAATTCCGGCCGGAGCGGTGGCTGACGGCGGATGGTAAGAAATTCATACCGTACGATTCGTACAAATTCGTAGCGTTCAATGCAGGGCCGAGGATCTGTTTGGGGAAGGATTTGGCGTATTTACAGATGAAGTCGATCGCAGCGGCGGTGCTGCTCCGGCACCGGCTGACGGTGGCGGCGGGGCATAAGGTGGAGCAGAAAATGTCGTTGACGCTGTTTATGAAGTACGGGCTGAAGGTGAACGTTCACCGGAGGGACTTGGAGGGGATTGTCGAAAGTATCAGGGGCAAAAACGGAATTTCGCAAGGGATAGATGCTCCGTTAATCGCCGTTGTGAACTGTAAAGGCGGTTGTAACGGAGTTAACGGAGTATAA

Coding sequence (CDS)

ATGGACGTGGGATTGGCGTTGGTTATTCTAACGGCCGTAACGGCGTATCTGCTGTGGTTCACCTTCATTTCACGGTCCTTAAAAGGCCCTCAAATGTGGCCCTTACTCGGCAGCCTCCCCGGACTCATCGAGAACTGCGACCGTTTACACGACTGGATCTCCGACAACCTCCGCGCCTGCGGCGGCACCTACCAGACCTGCATCTGGGCCTTCCCTTTCTTCGCCACCAAACAGGGCCTCGTCACCGTCACCTGCGACCCCAGAAATCTCGAGCACATTCTCAAGACCCGTTTCGACAATTACCCCAAGGGACCCACTTGGCAAGCCGTTTTTCACGACCTCCTCGGCCAGGGCATCTTTAACTCCGACGGTGACACCTGGCTCTTCCAGAGAAAAACTGCCGCCTTGGAATTTACCACTAGGACCCTCCGCCAAGCCATGGCTCGCTGGGTCAGCCGAGCCATTAAGCTTAGATTTTGCCCTATTCTCAAACAAGCCGAGCGAAATTCTAACCCCATTGATCTTCAGGATGTCTTGCTACGCCTTACTTTTGATAACATCTGCGGCTTGGCTTTCGGTAAGGACCCCATGACTTGCGCGCCCGGTTTCCCCGAGAACGGCTTTGCCACGGCTTTCGACCGAGCCACCGAGGCTTCCTTGCAACGCTTCATTTTGCCCGAAGTGCTTTGGAAATTCAAGAAGTGGCTCCGGCTTGGAATGGAGGTCAGCTTGAGCCGGAGCCTCGTCCATATTGAGGAGTACTTATCCAGTGTGATCGACTCACGTAAGCTCGAGTTGATGAATCAGCCTAAAGACTCCACCCCACACGACGATTTATTGTCGCGTTTCATGAAGAAGAAGGAAAGTAACTACTCCAACGCCTTCCTCCGCCACGTGGCGCTTAATTTCATCCTAGCTGGACGGGACACGTCGTCTGTCGCGCTGAGCTGGTTTTTCTGGTTGGTGACATTAAACCCACTCGTAGAGGAAAAAATCCTCCGCGAGATCTGCACCGTTCTGATTGAGACACGTGGCAATAACATCCCTTCATGGCTTGACGAGCCGTTAGGGTTCGAAGAGATCGACCGTCTGATTTATCTCAAAGCCGCTCTCACCGAAACCCTAAGACTGTACCCGTCGGTGCCGGAGGATTCAAAGCACGTCGTCGCCGACGACGTTCTGCCGGACGGTACCTTCGTTCCGGCGGGATCTTCGGTGACTTATTCGATCTACTCCACCGGAAGAATGAAGTCGACTTGGGGAGAGGACTGCCTCGAATTCCGGCCGGAGCGGTGGCTGACGGCGGATGGTAAGAAATTCATACCGTACGATTCGTACAAATTCGTAGCGTTCAATGCAGGGCCGAGGATCTGTTTGGGGAAGGATTTGGCGTATTTACAGATGAAGTCGATCGCAGCGGCGGTGCTGCTCCGGCACCGGCTGACGGTGGCGGCGGGGCATAAGGTGGAGCAGAAAATGTCGTTGACGCTGTTTATGAAGTACGGGCTGAAGGTGAACGTTCACCGGAGGGACTTGGAGGGGATTGTCGAAAGTATCAGGGGCAAAAACGGAATTTCGCAAGGGATAGATGCTCCGTTAATCGCCGTTGTGAACTGTAAAGGCGGTTGTAACGGAGTTAACGGAGTATAA

Protein sequence

MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRACGGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLLRLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGMEVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLRHVALNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEEIDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTWGEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHRLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKNGISQGIDAPLIAVVNCKGGCNGVNGV
Homology
BLAST of Spg023450 vs. NCBI nr
Match: XP_022958814.1 (cytochrome P450 86A8 [Cucurbita moschata])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 527/549 (95.99%), Postives = 534/549 (97.27%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGL LVI+ AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFH+LLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAE NS PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFA AFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLEL+NQ KDSTPHDDLLSRFMKKKESNYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQLKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNP +EEKILREICTVLIETRGNNI SWLDEPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPQIEEKILREICTVLIETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480
           GEDCLEFRPERWLTADGKKFIPYD YKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKNGISQGIDAPLIAVVNCK 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVHRR+LEGIVESIRGKNG SQG D PL+AVVNC 
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRELEGIVESIRGKNGNSQGNDTPLVAVVNCN 540

Query: 541 GGCNGVNGV 550
           GGCNG N V
Sbjct: 541 GGCNGFNDV 549

BLAST of Spg023450 vs. NCBI nr
Match: XP_023547593.1 (cytochrome P450 86A8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 527/549 (95.99%), Postives = 534/549 (97.27%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGLALVI+ AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLALVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFH+LLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAE NS PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFA AFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLEL+NQ KDSTPHDDLLSRFMKKKESNYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQLKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNP +EEKILREICTVLIETRGNNI SWLDEPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPQIEEKILREICTVLIETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480
           GEDCLEFRPERWLTADGKKFIPYD YKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKNGISQGIDAPLIAVVNCK 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVHRR+LEGIVESIRGK G SQG D PL+AVVNC 
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRELEGIVESIRGKKGNSQGNDTPLVAVVNCN 540

Query: 541 GGCNGVNGV 550
           GGCNG N V
Sbjct: 541 GGCNGFNDV 549

BLAST of Spg023450 vs. NCBI nr
Match: XP_023006552.1 (cytochrome P450 86A8 [Cucurbita maxima])

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 525/549 (95.63%), Postives = 534/549 (97.27%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGLALVI+ AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLALVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCIWAFP+FATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFH+LLGQGIF
Sbjct: 61  GGTYQTCIWAFPYFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAE NS PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFA AFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLEL+NQ KDSTPHDDLLSRFMKKKESNYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQLKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNP +EEKILREICTVLIETRGNNI SWLDEPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPQIEEKILREICTVLIETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480
           GEDCLEFRPERWLTADGKKFIPYD YKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKNGISQGIDAPLIAVVNCK 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVHRR+LEGIVE +RGKNG SQG D PL+AVVNC 
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRELEGIVEILRGKNGNSQGKDTPLVAVVNCN 540

Query: 541 GGCNGVNGV 550
           GGCNG N V
Sbjct: 541 GGCNGFNDV 549

BLAST of Spg023450 vs. NCBI nr
Match: XP_008458223.1 (PREDICTED: cytochrome P450 86A8-like [Cucumis melo] >TYK03018.1 cytochrome P450 86A8-like [Cucumis melo var. makuwa])

HSP 1 Score: 1072.0 bits (2771), Expect = 1.7e-309
Identity = 519/549 (94.54%), Postives = 533/549 (97.09%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVG ALVI+TAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCIWAFPFFA KQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFH+LLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILK+AE NS+PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKEAELNSHPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFA AFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLEL+NQPKDSTPHDDLLSRFMKKK+ NYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKDGNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNPL+EEKILREICTVL+ETRGNNI SWLDEPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLLETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480
           GEDCLEFRPERWLTADGKKFI YD YKFV+FNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIQYDPYKFVSFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKNGISQGIDAPLIAVVNCK 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVH+R+LEG+VES+RGK G S G D PLIAVVNC 
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHKRNLEGMVESMRGKYGNSHGKDGPLIAVVNCN 540

Query: 541 GGCNGVNGV 550
           GGCNGV  V
Sbjct: 541 GGCNGVKEV 549

BLAST of Spg023450 vs. NCBI nr
Match: XP_038907189.1 (cytochrome P450 86A8-like [Benincasa hispida])

HSP 1 Score: 1069.3 bits (2764), Expect = 1.1e-308
Identity = 522/549 (95.08%), Postives = 528/549 (96.17%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVG ALVI+ AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 23  MDVGFALVIVAAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 82

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCIWAFPFFA KQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFH+LLGQGIF
Sbjct: 83  GGTYQTCIWAFPFFAKKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 142

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAE NS PIDLQDVLL
Sbjct: 143 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAEFNSEPIDLQDVLL 202

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFA AFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 203 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 262

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300
           EVSL RSLVHIEEYLSSVIDSRKLEL+NQPKDSTPHDDLLSRFMKKKE NYSNAFLRHVA
Sbjct: 263 EVSLGRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 322

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNPL+EEKILREICTVLIETRGNNI SWL+EPL FEE
Sbjct: 323 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLAFEE 382

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 383 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 442

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480
           GEDCLEFRPERWLTADGKKFIPYD YKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 443 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 502

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKNGISQGIDAPLIAVVNCK 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGK G SQ  D PL+ VVN  
Sbjct: 503 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSQEKDTPLVPVVNGN 562

Query: 541 GGCNGVNGV 550
           GGCNG N V
Sbjct: 563 GGCNGFNEV 571

BLAST of Spg023450 vs. ExPASy Swiss-Prot
Match: O80823 (Cytochrome P450 86A8 OS=Arabidopsis thaliana OX=3702 GN=CYP86A8 PE=2 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 1.0e-248
Identity = 413/532 (77.63%), Postives = 464/532 (87.22%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           M++  AL+IL+A+TAY LW TFISR LKGP++WP+LGSLPGLIENC+R+HDWISDNLRAC
Sbjct: 1   MEISTALMILSAITAYFLWLTFISRCLKGPRVWPILGSLPGLIENCERMHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
            GTYQTCI A PF A KQGLVTVTCDPRNLEHILK RFDNYPKGPTWQAVFHDLLGQGIF
Sbjct: 61  SGTYQTCICAIPFLAKKQGLVTVTCDPRNLEHILKNRFDNYPKGPTWQAVFHDLLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWV+RAIKLRF PIL+ A   S PIDLQD+LL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVNRAIKLRFLPILENARLGSEPIDLQDLLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGL FGKDP TCAPG P N FA AFDRATEASLQRFILPE+LWKFK+WLRLG+
Sbjct: 181 RLTFDNICGLTFGKDPRTCAPGLPVNTFAVAFDRATEASLQRFILPEILWKFKRWLRLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300
           EVSL+RSLV ++ YLS +I +RK E+M Q  +   HDDLLSRF+KKKES YS+  L+ VA
Sbjct: 241 EVSLTRSLVQVDNYLSEIITTRKEEMMTQHNNGKHHDDLLSRFIKKKES-YSDETLQRVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEE 360
           LNFILAGRDTSSVALSWFFWL+T +P +E+KILREICTVL+ETRG+++  W DEPL  EE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLITQHPAIEDKILREICTVLVETRGDDVALWTDEPLSCEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           +DRL++LKAAL+ETLRLYPSVPEDSK  V DDVLPDGTFVPAGSS+TYSIYS GRMKSTW
Sbjct: 361 LDRLVFLKAALSETLRLYPSVPEDSKRAVKDDVLPDGTFVPAGSSITYSIYSAGRMKSTW 420

Query: 421 GEDCLEFRPERWLT-ADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRH 480
           GEDCLEF+PERW++ +DG +FI +D +KFVAFNAGPRICLGKDLAYLQMKSIA+AVLLRH
Sbjct: 421 GEDCLEFKPERWISQSDGGRFINHDPFKFVAFNAGPRICLGKDLAYLQMKSIASAVLLRH 480

Query: 481 RLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIR--GKNGISQGI 530
           RLTV  GHKVEQKMSLTLFMKYGL VNVH RDL  I   +R    N ++ G+
Sbjct: 481 RLTVVTGHKVEQKMSLTLFMKYGLLVNVHERDLTAIAADLRECKSNVVNDGV 531

BLAST of Spg023450 vs. ExPASy Swiss-Prot
Match: O23066 (Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1)

HSP 1 Score: 850.1 bits (2195), Expect = 1.4e-245
Identity = 410/546 (75.09%), Postives = 466/546 (85.35%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDV   ++++  V AY LWF  ISR LKGP++WP+LGSLPGLIE  DR+HDWI++NLRAC
Sbjct: 1   MDVSNTMLLVAVVAAYWLWFQRISRWLKGPRVWPVLGSLPGLIEQRDRMHDWITENLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCI A PF A KQGLVTVTCDP+N+EH+LKTRFDNYPKGPTWQAVFHD LGQGIF
Sbjct: 61  GGTYQTCICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAM RWV+R IKLRFCPIL+ A+ N  P+DLQD++L
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQDLIL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKD  TCAPG PENGFA+AFDRATEASLQRFILPE LW+ KKWL LG+
Sbjct: 181 RLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLGLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTP-HDDLLSRFMKKKESNYSNAFLRHV 300
           EVSLSRSL  I+ YL +VI++RK EL++Q +     HDDLLSRFMKKK+ +YS  FLRHV
Sbjct: 241 EVSLSRSLGEIDGYLDAVINTRKQELLSQRESGVQRHDDLLSRFMKKKDQSYSETFLRHV 300

Query: 301 ALNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFE 360
           ALNFILAGRDTSSVALSWFFWL+T +P VE+KI+REIC+VLIETRG ++ SW  EPL F+
Sbjct: 301 ALNFILAGRDTSSVALSWFFWLITTHPTVEDKIVREICSVLIETRGTDVSSWTAEPLEFD 360

Query: 361 EIDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKST 420
           E+DRL+YLKAAL+ETLRLYPSVPEDSKHVV DD+LPDGTFVPAGSSVTYSIY+ GRMKST
Sbjct: 361 EVDRLVYLKAALSETLRLYPSVPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKST 420

Query: 421 WGEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRH 480
           WGEDCLEF+PERW++ D  KF+ +D Y+FVAFNAGPRICLGKDLAYLQMK+IAAAVLLRH
Sbjct: 421 WGEDCLEFKPERWISPDDGKFVNHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRH 480

Query: 481 RLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKNGISQGIDAPLIAVVNC 540
           RLTVA GHKVEQKMSLTLFMK GL VNVH+RDLE +++S+  K             VV  
Sbjct: 481 RLTVAPGHKVEQKMSLTLFMKNGLLVNVHKRDLEVMMKSLVPKERND---------VVVL 537

Query: 541 KGGCNG 546
            G CNG
Sbjct: 541 NGKCNG 537

BLAST of Spg023450 vs. ExPASy Swiss-Prot
Match: Q9LMM1 (Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 6.8e-245
Identity = 410/551 (74.41%), Postives = 475/551 (86.21%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           M++  A++++  VT Y LWF  ISR LKGP++WPLLGSLPGLIE  DR+H+WI++NLRAC
Sbjct: 1   MEISNAMLLVAIVTGYWLWFKRISRWLKGPRVWPLLGSLPGLIEQRDRMHEWITENLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCI+A PF A KQGLVTVTCDP+NLEH+LKTRFDNYPKGPTWQ+VFHDLLGQGIF
Sbjct: 61  GGTYQTCIFAVPFLAKKQGLVTVTCDPKNLEHMLKTRFDNYPKGPTWQSVFHDLLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAM RWV+R IKLRFCPIL  A+ N+ P+DLQD++L
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILATAQDNAEPVDLQDLIL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKD  TCAPG PENGFA+AFDRATEASLQRFI+P+ +WK KKWL LG+
Sbjct: 181 RLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFIIPKFMWKLKKWLGLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDST--PHDDLLSRFMKKKESNYSNAFLRH 300
           EVSLSRSL  I+EYL++VI++RK ELM+Q +  T   HDDLLSRFM KK  +YS+ FL+H
Sbjct: 241 EVSLSRSLGEIDEYLAAVINTRKQELMSQQESGTHQRHDDLLSRFMMKKTESYSDTFLQH 300

Query: 301 VALNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRG-NNIPSWLDEPLG 360
           VALNFILAGRDTSSVALSWFFWL+T++P VE+KI+REIC+VLIETRG +++ SW +EPLG
Sbjct: 301 VALNFILAGRDTSSVALSWFFWLITMHPTVEDKIVREICSVLIETRGTDDVASWTEEPLG 360

Query: 361 FEEIDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMK 420
           F+EIDRL+YLKAA++ETLRLYPSVPEDSKHV  DDVLPDGTFVPAGSSVTYSIY+ GRMK
Sbjct: 361 FDEIDRLVYLKAAISETLRLYPSVPEDSKHVENDDVLPDGTFVPAGSSVTYSIYAAGRMK 420

Query: 421 STWGEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLL 480
           STWGEDCLEF PERW++    KFI +D Y+FVAFNAGPRICLGKDLAYLQMK+IAAAVLL
Sbjct: 421 STWGEDCLEFNPERWISPIDGKFINHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLL 480

Query: 481 RHRLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKNGISQGIDAPLIAVV 540
           RHRLTV  GHKVEQKMSLTLFMK GL VN+++RDL+GI++S+  K   S G+        
Sbjct: 481 RHRLTVVPGHKVEQKMSLTLFMKNGLLVNLYKRDLQGIIKSLVVKK--SDGVS------- 540

Query: 541 NCKGGCNGVNG 549
              G CNGV G
Sbjct: 541 --NGQCNGVIG 540

BLAST of Spg023450 vs. ExPASy Swiss-Prot
Match: B3RFJ6 (Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 3.0e-232
Identity = 398/551 (72.23%), Postives = 457/551 (82.94%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQ---MWPLLGSLPGLIENCDRLHDWISDNL 60
           M+V   ++I+  V AYLLWF  I++S+KGP+   MWP++GSLPGLIEN +R+H+WI+DNL
Sbjct: 1   MEVSTTMMIVAIVAAYLLWFKSITKSMKGPKGPTMWPVVGSLPGLIENGNRMHEWIADNL 60

Query: 61  RACGGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQ 120
           RAC GTYQTCI A PF A KQ LVTVTCDP+NLEHILK RFDNYPKGPTWQAVFHDLLG+
Sbjct: 61  RACSGTYQTCICAIPFLARKQ-LVTVTCDPKNLEHILKVRFDNYPKGPTWQAVFHDLLGE 120

Query: 121 GIFNSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQD 180
           GIFNSDGDTWLFQRKTAALEFTTRTLRQAM RWV+RAIK RFCPIL+ A+    P+DLQD
Sbjct: 121 GIFNSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRAIKNRFCPILEMAQVQGKPVDLQD 180

Query: 181 VLLRLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLR 240
           +LLRLTFDNICGLAFGKDP T +P  PEN FAT+FDRATEA+L RFI+PE +WK KK L 
Sbjct: 181 LLLRLTFDNICGLAFGKDPETLSPELPENNFATSFDRATEATLHRFIMPEFVWKLKKMLG 240

Query: 241 LGMEVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLR 300
           LG+EVSL+ SL  ++ Y++ VI++RKLEL+N       HDDLLSRFMKKKES YS+ FL+
Sbjct: 241 LGLEVSLNNSLKQVDNYMTDVINTRKLELLNHQNGGPQHDDLLSRFMKKKES-YSDKFLQ 300

Query: 301 HVALNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLG 360
           HVALNFILAGRDTSSVALSWFFWLV+ NP VEEKIL EICT+L ETRGN+   WL+EPL 
Sbjct: 301 HVALNFILAGRDTSSVALSWFFWLVSSNPRVEEKILVEICTILAETRGNDTSKWLEEPLV 360

Query: 361 FEEIDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMK 420
           FEE+D+L+YLKAAL+ETLRLYPSVPEDSKHV++DD LPDGTFVPAGS++TYSIYSTGRMK
Sbjct: 361 FEEVDQLMYLKAALSETLRLYPSVPEDSKHVISDDYLPDGTFVPAGSNITYSIYSTGRMK 420

Query: 421 STWGEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLL 480
             WGEDCLEF+PERW++ DG KF   D+++FVAFNAGPRICLGKDLAYLQMKSIAAAVLL
Sbjct: 421 FIWGEDCLEFKPERWMSQDGDKFQVQDTFRFVAFNAGPRICLGKDLAYLQMKSIAAAVLL 480

Query: 481 RHRLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLE---------GIVESIRGKNG-ISQ 539
           RHRL VA GHKVEQKMSLTLFMK GL +NV  RDL          G VES  G++  I+ 
Sbjct: 481 RHRLAVAPGHKVEQKMSLTLFMKDGLVMNVTPRDLTPILAKIEKFGKVESCAGEHHLINN 540

BLAST of Spg023450 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 1.4e-229
Identity = 379/519 (73.03%), Postives = 441/519 (84.97%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MD   A +ILT +  Y++WF  + RS KGP++WPL+GSLP LI N  R+HD+I+DNLR C
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAHRMHDFIADNLRMC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCI+  PF A KQG VTVTCDP+NLEHILKTRFDNYPKGP+WQ+VFHDLLG GIF
Sbjct: 61  GGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTW FQRKTAALEFTTRTLRQAMARWV RAIK R  PIL+ A   + PIDLQDVLL
Sbjct: 121 NSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGL FGKDP T +P FPENGFA AFD ATEA+LQRFI+PE +WK +KWLRLG+
Sbjct: 181 RLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300
           E  +SRS+ H++ YLS +I++RKLEL+ Q +D + HDDLLSRFMKKKES YS+ +L++VA
Sbjct: 241 EDDMSRSISHVDNYLSEIINTRKLELLGQQQDESRHDDLLSRFMKKKES-YSDKYLKYVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEE 360
           LNFILAGRDTSSVA+SWFFWLV+LNP VEEKI+ EICT+LI+TR  N+  W DEPL F+E
Sbjct: 301 LNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDEPLTFDE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           ID+L+YLKAAL+ETLRLYPSVPEDSK VVA+DVLPDGTFVP+GS+VTYSIYS GRMK  W
Sbjct: 361 IDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYSVGRMKFIW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480
           GEDCLEF+PERWL  +  +    + YKFVAFNAGPRICLGKDLAYLQMKSI A++LLRHR
Sbjct: 421 GEDCLEFKPERWL--EESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITASILLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESI 520
           LTVA GH+VEQKMSLTLFMK+GLK++VH+RDL   VE +
Sbjct: 481 LTVAPGHRVEQKMSLTLFMKFGLKMDVHKRDLTLPVEKV 516

BLAST of Spg023450 vs. ExPASy TrEMBL
Match: A0A6J1H676 (cytochrome P450 86A8 OS=Cucurbita moschata OX=3662 GN=LOC111459971 PE=3 SV=1)

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 527/549 (95.99%), Postives = 534/549 (97.27%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGL LVI+ AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFH+LLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAE NS PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFA AFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLEL+NQ KDSTPHDDLLSRFMKKKESNYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQLKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNP +EEKILREICTVLIETRGNNI SWLDEPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPQIEEKILREICTVLIETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480
           GEDCLEFRPERWLTADGKKFIPYD YKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKNGISQGIDAPLIAVVNCK 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVHRR+LEGIVESIRGKNG SQG D PL+AVVNC 
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRELEGIVESIRGKNGNSQGNDTPLVAVVNCN 540

Query: 541 GGCNGVNGV 550
           GGCNG N V
Sbjct: 541 GGCNGFNDV 549

BLAST of Spg023450 vs. ExPASy TrEMBL
Match: A0A6J1KW55 (cytochrome P450 86A8 OS=Cucurbita maxima OX=3661 GN=LOC111499241 PE=3 SV=1)

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 525/549 (95.63%), Postives = 534/549 (97.27%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGLALVI+ AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLALVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCIWAFP+FATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFH+LLGQGIF
Sbjct: 61  GGTYQTCIWAFPYFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAE NS PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFA AFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLEL+NQ KDSTPHDDLLSRFMKKKESNYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQLKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNP +EEKILREICTVLIETRGNNI SWLDEPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPQIEEKILREICTVLIETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480
           GEDCLEFRPERWLTADGKKFIPYD YKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKNGISQGIDAPLIAVVNCK 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVHRR+LEGIVE +RGKNG SQG D PL+AVVNC 
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRELEGIVEILRGKNGNSQGKDTPLVAVVNCN 540

Query: 541 GGCNGVNGV 550
           GGCNG N V
Sbjct: 541 GGCNGFNDV 549

BLAST of Spg023450 vs. ExPASy TrEMBL
Match: A0A5D3BVJ6 (Cytochrome P450 86A8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001130 PE=3 SV=1)

HSP 1 Score: 1072.0 bits (2771), Expect = 8.2e-310
Identity = 519/549 (94.54%), Postives = 533/549 (97.09%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVG ALVI+TAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCIWAFPFFA KQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFH+LLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILK+AE NS+PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKEAELNSHPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFA AFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLEL+NQPKDSTPHDDLLSRFMKKK+ NYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKDGNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNPL+EEKILREICTVL+ETRGNNI SWLDEPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLLETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480
           GEDCLEFRPERWLTADGKKFI YD YKFV+FNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIQYDPYKFVSFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKNGISQGIDAPLIAVVNCK 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVH+R+LEG+VES+RGK G S G D PLIAVVNC 
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHKRNLEGMVESMRGKYGNSHGKDGPLIAVVNCN 540

Query: 541 GGCNGVNGV 550
           GGCNGV  V
Sbjct: 541 GGCNGVKEV 549

BLAST of Spg023450 vs. ExPASy TrEMBL
Match: A0A1S3C7C5 (cytochrome P450 86A8-like OS=Cucumis melo OX=3656 GN=LOC103497711 PE=3 SV=1)

HSP 1 Score: 1072.0 bits (2771), Expect = 8.2e-310
Identity = 519/549 (94.54%), Postives = 533/549 (97.09%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVG ALVI+TAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCIWAFPFFA KQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFH+LLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILK+AE NS+PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKEAELNSHPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFA AFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLEL+NQPKDSTPHDDLLSRFMKKK+ NYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKDGNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNPL+EEKILREICTVL+ETRGNNI SWLDEPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLLETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480
           GEDCLEFRPERWLTADGKKFI YD YKFV+FNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIQYDPYKFVSFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKNGISQGIDAPLIAVVNCK 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVH+R+LEG+VES+RGK G S G D PLIAVVNC 
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHKRNLEGMVESMRGKYGNSHGKDGPLIAVVNCN 540

Query: 541 GGCNGVNGV 550
           GGCNGV  V
Sbjct: 541 GGCNGVKEV 549

BLAST of Spg023450 vs. ExPASy TrEMBL
Match: A0A0A0K9Z1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G004550 PE=3 SV=1)

HSP 1 Score: 1067.4 bits (2759), Expect = 2.0e-308
Identity = 516/546 (94.51%), Postives = 531/546 (97.25%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVG ALVI+TAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCIWAFPFFA KQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFH+LLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILK+AE NS+PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKEAELNSHPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFA AFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLEL+NQPKDSTPHDDLLSRFMKKK+ NYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKDGNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNPL+EEKILREICTVLIETRGNNI SWLDEPL FEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLDEPLEFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480
           GEDCLEFRPERWLTADG KFI YD YKFV+FNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGTKFIAYDPYKFVSFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKNGISQGIDAPLIAVVNCK 540
           LTVAAGHKVEQKMSLTLFMKYGLKVN+H+R+LEGIVES+RGK G SQG D+PLI VVNC 
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNIHKRNLEGIVESMRGKYGNSQGKDSPLITVVNCN 540

Query: 541 GGCNGV 547
           GGCNG+
Sbjct: 541 GGCNGM 546

BLAST of Spg023450 vs. TAIR 10
Match: AT2G45970.1 (cytochrome P450, family 86, subfamily A, polypeptide 8 )

HSP 1 Score: 860.5 bits (2222), Expect = 7.2e-250
Identity = 413/532 (77.63%), Postives = 464/532 (87.22%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           M++  AL+IL+A+TAY LW TFISR LKGP++WP+LGSLPGLIENC+R+HDWISDNLRAC
Sbjct: 1   MEISTALMILSAITAYFLWLTFISRCLKGPRVWPILGSLPGLIENCERMHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
            GTYQTCI A PF A KQGLVTVTCDPRNLEHILK RFDNYPKGPTWQAVFHDLLGQGIF
Sbjct: 61  SGTYQTCICAIPFLAKKQGLVTVTCDPRNLEHILKNRFDNYPKGPTWQAVFHDLLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWV+RAIKLRF PIL+ A   S PIDLQD+LL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVNRAIKLRFLPILENARLGSEPIDLQDLLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGL FGKDP TCAPG P N FA AFDRATEASLQRFILPE+LWKFK+WLRLG+
Sbjct: 181 RLTFDNICGLTFGKDPRTCAPGLPVNTFAVAFDRATEASLQRFILPEILWKFKRWLRLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300
           EVSL+RSLV ++ YLS +I +RK E+M Q  +   HDDLLSRF+KKKES YS+  L+ VA
Sbjct: 241 EVSLTRSLVQVDNYLSEIITTRKEEMMTQHNNGKHHDDLLSRFIKKKES-YSDETLQRVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEE 360
           LNFILAGRDTSSVALSWFFWL+T +P +E+KILREICTVL+ETRG+++  W DEPL  EE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLITQHPAIEDKILREICTVLVETRGDDVALWTDEPLSCEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           +DRL++LKAAL+ETLRLYPSVPEDSK  V DDVLPDGTFVPAGSS+TYSIYS GRMKSTW
Sbjct: 361 LDRLVFLKAALSETLRLYPSVPEDSKRAVKDDVLPDGTFVPAGSSITYSIYSAGRMKSTW 420

Query: 421 GEDCLEFRPERWLT-ADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRH 480
           GEDCLEF+PERW++ +DG +FI +D +KFVAFNAGPRICLGKDLAYLQMKSIA+AVLLRH
Sbjct: 421 GEDCLEFKPERWISQSDGGRFINHDPFKFVAFNAGPRICLGKDLAYLQMKSIASAVLLRH 480

Query: 481 RLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIR--GKNGISQGI 530
           RLTV  GHKVEQKMSLTLFMKYGL VNVH RDL  I   +R    N ++ G+
Sbjct: 481 RLTVVTGHKVEQKMSLTLFMKYGLLVNVHERDLTAIAADLRECKSNVVNDGV 531

BLAST of Spg023450 vs. TAIR 10
Match: AT4G00360.1 (cytochrome P450, family 86, subfamily A, polypeptide 2 )

HSP 1 Score: 850.1 bits (2195), Expect = 9.7e-247
Identity = 410/546 (75.09%), Postives = 466/546 (85.35%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDV   ++++  V AY LWF  ISR LKGP++WP+LGSLPGLIE  DR+HDWI++NLRAC
Sbjct: 1   MDVSNTMLLVAVVAAYWLWFQRISRWLKGPRVWPVLGSLPGLIEQRDRMHDWITENLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCI A PF A KQGLVTVTCDP+N+EH+LKTRFDNYPKGPTWQAVFHD LGQGIF
Sbjct: 61  GGTYQTCICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAM RWV+R IKLRFCPIL+ A+ N  P+DLQD++L
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQDLIL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKD  TCAPG PENGFA+AFDRATEASLQRFILPE LW+ KKWL LG+
Sbjct: 181 RLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLGLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTP-HDDLLSRFMKKKESNYSNAFLRHV 300
           EVSLSRSL  I+ YL +VI++RK EL++Q +     HDDLLSRFMKKK+ +YS  FLRHV
Sbjct: 241 EVSLSRSLGEIDGYLDAVINTRKQELLSQRESGVQRHDDLLSRFMKKKDQSYSETFLRHV 300

Query: 301 ALNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFE 360
           ALNFILAGRDTSSVALSWFFWL+T +P VE+KI+REIC+VLIETRG ++ SW  EPL F+
Sbjct: 301 ALNFILAGRDTSSVALSWFFWLITTHPTVEDKIVREICSVLIETRGTDVSSWTAEPLEFD 360

Query: 361 EIDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKST 420
           E+DRL+YLKAAL+ETLRLYPSVPEDSKHVV DD+LPDGTFVPAGSSVTYSIY+ GRMKST
Sbjct: 361 EVDRLVYLKAALSETLRLYPSVPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKST 420

Query: 421 WGEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRH 480
           WGEDCLEF+PERW++ D  KF+ +D Y+FVAFNAGPRICLGKDLAYLQMK+IAAAVLLRH
Sbjct: 421 WGEDCLEFKPERWISPDDGKFVNHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRH 480

Query: 481 RLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKNGISQGIDAPLIAVVNC 540
           RLTVA GHKVEQKMSLTLFMK GL VNVH+RDLE +++S+  K             VV  
Sbjct: 481 RLTVAPGHKVEQKMSLTLFMKNGLLVNVHKRDLEVMMKSLVPKERND---------VVVL 537

Query: 541 KGGCNG 546
            G CNG
Sbjct: 541 NGKCNG 537

BLAST of Spg023450 vs. TAIR 10
Match: AT1G01600.1 (cytochrome P450, family 86, subfamily A, polypeptide 4 )

HSP 1 Score: 847.8 bits (2189), Expect = 4.8e-246
Identity = 410/551 (74.41%), Postives = 475/551 (86.21%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           M++  A++++  VT Y LWF  ISR LKGP++WPLLGSLPGLIE  DR+H+WI++NLRAC
Sbjct: 1   MEISNAMLLVAIVTGYWLWFKRISRWLKGPRVWPLLGSLPGLIEQRDRMHEWITENLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCI+A PF A KQGLVTVTCDP+NLEH+LKTRFDNYPKGPTWQ+VFHDLLGQGIF
Sbjct: 61  GGTYQTCIFAVPFLAKKQGLVTVTCDPKNLEHMLKTRFDNYPKGPTWQSVFHDLLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAM RWV+R IKLRFCPIL  A+ N+ P+DLQD++L
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILATAQDNAEPVDLQDLIL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKD  TCAPG PENGFA+AFDRATEASLQRFI+P+ +WK KKWL LG+
Sbjct: 181 RLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFIIPKFMWKLKKWLGLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDST--PHDDLLSRFMKKKESNYSNAFLRH 300
           EVSLSRSL  I+EYL++VI++RK ELM+Q +  T   HDDLLSRFM KK  +YS+ FL+H
Sbjct: 241 EVSLSRSLGEIDEYLAAVINTRKQELMSQQESGTHQRHDDLLSRFMMKKTESYSDTFLQH 300

Query: 301 VALNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRG-NNIPSWLDEPLG 360
           VALNFILAGRDTSSVALSWFFWL+T++P VE+KI+REIC+VLIETRG +++ SW +EPLG
Sbjct: 301 VALNFILAGRDTSSVALSWFFWLITMHPTVEDKIVREICSVLIETRGTDDVASWTEEPLG 360

Query: 361 FEEIDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMK 420
           F+EIDRL+YLKAA++ETLRLYPSVPEDSKHV  DDVLPDGTFVPAGSSVTYSIY+ GRMK
Sbjct: 361 FDEIDRLVYLKAAISETLRLYPSVPEDSKHVENDDVLPDGTFVPAGSSVTYSIYAAGRMK 420

Query: 421 STWGEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLL 480
           STWGEDCLEF PERW++    KFI +D Y+FVAFNAGPRICLGKDLAYLQMK+IAAAVLL
Sbjct: 421 STWGEDCLEFNPERWISPIDGKFINHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLL 480

Query: 481 RHRLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKNGISQGIDAPLIAVV 540
           RHRLTV  GHKVEQKMSLTLFMK GL VN+++RDL+GI++S+  K   S G+        
Sbjct: 481 RHRLTVVPGHKVEQKMSLTLFMKNGLLVNLYKRDLQGIIKSLVVKK--SDGVS------- 540

Query: 541 NCKGGCNGVNG 549
              G CNGV G
Sbjct: 541 --NGQCNGVIG 540

BLAST of Spg023450 vs. TAIR 10
Match: AT1G63710.1 (cytochrome P450, family 86, subfamily A, polypeptide 7 )

HSP 1 Score: 797.0 bits (2057), Expect = 9.8e-231
Identity = 379/519 (73.03%), Postives = 441/519 (84.97%), Query Frame = 0

Query: 1   MDVGLALVILTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MD   A +ILT +  Y++WF  + RS KGP++WPL+GSLP LI N  R+HD+I+DNLR C
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAHRMHDFIADNLRMC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIF 120
           GGTYQTCI+  PF A KQG VTVTCDP+NLEHILKTRFDNYPKGP+WQ+VFHDLLG GIF
Sbjct: 61  GGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLL 180
           NSDGDTW FQRKTAALEFTTRTLRQAMARWV RAIK R  PIL+ A   + PIDLQDVLL
Sbjct: 121 NSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGL FGKDP T +P FPENGFA AFD ATEA+LQRFI+PE +WK +KWLRLG+
Sbjct: 181 RLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300
           E  +SRS+ H++ YLS +I++RKLEL+ Q +D + HDDLLSRFMKKKES YS+ +L++VA
Sbjct: 241 EDDMSRSISHVDNYLSEIINTRKLELLGQQQDESRHDDLLSRFMKKKES-YSDKYLKYVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEE 360
           LNFILAGRDTSSVA+SWFFWLV+LNP VEEKI+ EICT+LI+TR  N+  W DEPL F+E
Sbjct: 301 LNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDEPLTFDE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           ID+L+YLKAAL+ETLRLYPSVPEDSK VVA+DVLPDGTFVP+GS+VTYSIYS GRMK  W
Sbjct: 361 IDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYSVGRMKFIW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480
           GEDCLEF+PERWL  +  +    + YKFVAFNAGPRICLGKDLAYLQMKSI A++LLRHR
Sbjct: 421 GEDCLEFKPERWL--EESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITASILLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESI 520
           LTVA GH+VEQKMSLTLFMK+GLK++VH+RDL   VE +
Sbjct: 481 LTVAPGHRVEQKMSLTLFMKFGLKMDVHKRDLTLPVEKV 516

BLAST of Spg023450 vs. TAIR 10
Match: AT5G58860.1 (cytochrome P450, family 86, subfamily A, polypeptide 1 )

HSP 1 Score: 666.0 bits (1717), Expect = 2.6e-191
Identity = 328/505 (64.95%), Postives = 388/505 (76.83%), Query Frame = 0

Query: 10  LTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRACGGTYQTCIW 69
           + A++ Y LWF F+SR L GP++ P +GSLP LI N  R+HDWI+DNLRA GGTYQTC  
Sbjct: 13  VAALSVYALWFYFLSRRLTGPKVLPFVGSLPYLIANRSRIHDWIADNLRATGGTYQTCTM 72

Query: 70  AFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHDLLGQGIFNSDGDTWLF 129
             PF A  QG  TVTC P+N+EHILKTRFDNYPKGP W+A FHDLLGQGIFNSDGDTWL 
Sbjct: 73  VIPFVAKAQGFYTVTCHPKNVEHILKTRFDNYPKGPMWRAAFHDLLGQGIFNSDGDTWLM 132

Query: 130 QRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAERNSNPIDLQDVLLRLTFDNICG 189
           QRKTAALEFTTRTLRQAMARWV+  IK R   IL +A +N+ P+DLQD+ LRLTFDNICG
Sbjct: 133 QRKTAALEFTTRTLRQAMARWVNGTIKNRLWLILDRAVQNNKPVDLQDLFLRLTFDNICG 192

Query: 190 LAFGKDPMTCAPGFPENGFATAFDRATEASLQRFILPEVLWKFKKWLRLGMEVSLSRSLV 249
           L FGKDP T +   P+N F+ AFD ATEA+L+R +    LW+ +K + +G E  L +SL 
Sbjct: 193 LTFGKDPETLSLDLPDNPFSVAFDTATEATLKRLLYTGFLWRIQKAMGIGSEDKLKKSLE 252

Query: 250 HIEEYLSSVIDSRKLELMNQPKDSTPHDDLLSRFMKKKESN---YSNAFLRHVALNFILA 309
            +E Y++  ID+RK         ++P DDLLSRF+KK++ N        L+ +ALNF+LA
Sbjct: 253 VVETYMNDAIDARK---------NSPSDDLLSRFLKKRDVNGNVLPTDVLQRIALNFVLA 312

Query: 310 GRDTSSVALSWFFWLVTLNPLVEEKILREICTVLIETRGNNIPSWLDEPLGFEEIDRLIY 369
           GRDTSSVALSWFFWLV  N  VE KI+ E+  VL ETRGN+   W +EPL F+E DRL+Y
Sbjct: 313 GRDTSSVALSWFFWLVMNNREVETKIVNELSMVLKETRGNDQEKWTEEPLEFDEADRLVY 372

Query: 370 LKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTWGEDCLE 429
           LKAAL ETLRLYPSVP+D K+VV DDVLPDGTFVP GS+VTYSIYS GRMK+ WGEDCLE
Sbjct: 373 LKAALAETLRLYPSVPQDFKYVVDDDVLPDGTFVPRGSTVTYSIYSIGRMKTIWGEDCLE 432

Query: 430 FRPERWLTADGKKF-IPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHRLTVAA 489
           FRPERWLTADG++F  P D YKFVAFNAGPR CLGKDLAY QMKS+A+AVLLR+R+    
Sbjct: 433 FRPERWLTADGERFETPKDGYKFVAFNAGPRTCLGKDLAYNQMKSVASAVLLRYRVFPVP 492

Query: 490 GHKVEQKMSLTLFMKYGLKVNVHRR 511
           GH+VEQKMSLTLFMK GL+V +  R
Sbjct: 493 GHRVEQKMSLTLFMKNGLRVYLQPR 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022958814.10.0e+0095.99cytochrome P450 86A8 [Cucurbita moschata][more]
XP_023547593.10.0e+0095.99cytochrome P450 86A8 [Cucurbita pepo subsp. pepo][more]
XP_023006552.10.0e+0095.63cytochrome P450 86A8 [Cucurbita maxima][more]
XP_008458223.11.7e-30994.54PREDICTED: cytochrome P450 86A8-like [Cucumis melo] >TYK03018.1 cytochrome P450 ... [more]
XP_038907189.11.1e-30895.08cytochrome P450 86A8-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O808231.0e-24877.63Cytochrome P450 86A8 OS=Arabidopsis thaliana OX=3702 GN=CYP86A8 PE=2 SV=1[more]
O230661.4e-24575.09Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1[more]
Q9LMM16.8e-24574.41Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1[more]
B3RFJ63.0e-23272.23Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1[more]
Q9CAD61.4e-22973.03Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H6760.0e+0095.99cytochrome P450 86A8 OS=Cucurbita moschata OX=3662 GN=LOC111459971 PE=3 SV=1[more]
A0A6J1KW550.0e+0095.63cytochrome P450 86A8 OS=Cucurbita maxima OX=3661 GN=LOC111499241 PE=3 SV=1[more]
A0A5D3BVJ68.2e-31094.54Cytochrome P450 86A8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3C7C58.2e-31094.54cytochrome P450 86A8-like OS=Cucumis melo OX=3656 GN=LOC103497711 PE=3 SV=1[more]
A0A0A0K9Z12.0e-30894.51Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G004550 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G45970.17.2e-25077.63cytochrome P450, family 86, subfamily A, polypeptide 8 [more]
AT4G00360.19.7e-24775.09cytochrome P450, family 86, subfamily A, polypeptide 2 [more]
AT1G01600.14.8e-24674.41cytochrome P450, family 86, subfamily A, polypeptide 4 [more]
AT1G63710.19.8e-23173.03cytochrome P450, family 86, subfamily A, polypeptide 7 [more]
AT5G58860.12.6e-19164.95cytochrome P450, family 86, subfamily A, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 458..469
score: 38.77
coord: 369..380
score: 43.62
coord: 306..323
score: 34.14
coord: 449..458
score: 49.59
IPR001128Cytochrome P450PFAMPF00067p450coord: 28..497
e-value: 2.1E-61
score: 208.1
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 315..341
score: 28.54
coord: 368..386
score: 27.97
coord: 295..312
score: 29.22
coord: 448..458
score: 46.92
coord: 458..481
score: 24.93
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 24..510
e-value: 3.0E-96
score: 324.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 21..510
NoneNo IPR availablePANTHERPTHR24296:SF117CYTOCHROME P450 86A2coord: 7..523
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 7..523
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 451..460

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg023450.1Spg023450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen