Spg023391 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg023391
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	peptide chain release factor PrfB2, chloroplastic
Location	scaffold13: 9669458 .. 9669745 (+)
RNA-Seq Expression	Spg023391
Synteny	Spg023391

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGAGATTGGAGTTGTTGTCGGTGGAGTTGGAGAAGGCTGATCTGTGGGATGATGCTACACATGCAGGGAAGATAAGTCGGGAGCATGGTTCAATTATGGATAAGATTAAAGAGATCAAAGCATTTGAGAGAGAGTTGCTTGAGCATATCGACATGATAAAGCTTGCCAGTGAGGGAGAGAGATCGATCTTGAAAAACCCAGATCTGGAAAACTCTTCAATCCATTTGTCTACTACGCACAAATCTGGAAAAACTCTCGAGAGGGAGACAGAGACCGAGAGTTAG

mRNA sequence

ATGATGAGATTGGAGTTGTTGTCGGTGGAGTTGGAGAAGGCTGATCTGTGGGATGATGCTACACATGCAGGGAAGATAAGTCGGGAGCATGGTTCAATTATGGATAAGATTAAAGAGATCAAAGCATTTGAGAGAGAGTTGCTTGAGCATATCGACATGATAAAGCTTGCCAGTGAGGGAGAGAGATCGATCTTGAAAAACCCAGATCTGGAAAACTCTTCAATCCATTTGTCTACTACGCACAAATCTGGAAAAACTCTCGAGAGGGAGACAGAGACCGAGAGTTAG

Coding sequence (CDS)

ATGATGAGATTGGAGTTGTTGTCGGTGGAGTTGGAGAAGGCTGATCTGTGGGATGATGCTACACATGCAGGGAAGATAAGTCGGGAGCATGGTTCAATTATGGATAAGATTAAAGAGATCAAAGCATTTGAGAGAGAGTTGCTTGAGCATATCGACATGATAAAGCTTGCCAGTGAGGGAGAGAGATCGATCTTGAAAAACCCAGATCTGGAAAACTCTTCAATCCATTTGTCTACTACGCACAAATCTGGAAAAACTCTCGAGAGGGAGACAGAGACCGAGAGTTAG

Protein sequence

MMRLELLSVELEKADLWDDATHAGKISREHGSIMDKIKEIKAFERELLEHIDMIKLASEGERSILKNPDLENSSIHLSTTHKSGKTLERETETES

Homology

[Close]

BLAST of Spg023391 vs. NCBI nr
Match: XP_023531900.1 (peptide chain release factor PrfB2, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023531901.1 peptide chain release factor PrfB2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 105.5 bits (262), Expect = 2.5e-19
Identity = 61/91 (67.03%), Postives = 69/91 (75.82%), Query Frame = 0


Query: 1   MMRLELLSVELEKADLWDDATHAGKISREHGSIMDKIKEIKAFERELLEHIDMIKLASEG 60
           MMRLELLSVEL KADLWDD  HAGKISREHGSIM KIKEIKAFERELLEHIDMIKLA E 
Sbjct: 129 MMRLELLSVELNKADLWDDPAHAGKISREHGSIMGKIKEIKAFERELLEHIDMIKLAREE 188

Query: 61  ERSILKNPDLENSSIH-LSTTHKSGKTLERE 91
                 + +LE+ S++ L +  ++ K  E E
Sbjct: 189 -----NDAELESESLNALLSMRRNSKEKELE 214

[Close]

BLAST of Spg023391 vs. NCBI nr
Match: XP_022931672.1 (peptide chain release factor PrfB2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 105.5 bits (262), Expect = 2.5e-19
Identity = 61/91 (67.03%), Postives = 69/91 (75.82%), Query Frame = 0


Query: 1   MMRLELLSVELEKADLWDDATHAGKISREHGSIMDKIKEIKAFERELLEHIDMIKLASEG 60
           MMRLELLSVEL KADLWDD  HAGKISREHGSIM KIKEIKAFERELLEHIDMIKLA E 
Sbjct: 129 MMRLELLSVELNKADLWDDPAHAGKISREHGSIMGKIKEIKAFERELLEHIDMIKLAREE 188

Query: 61  ERSILKNPDLENSSIH-LSTTHKSGKTLERE 91
                 + +LE+ S++ L +  ++ K  E E
Sbjct: 189 -----NDAELESESLNALLSMRRNSKEKELE 214

[Close]

BLAST of Spg023391 vs. NCBI nr
Match: XP_038878322.1 (peptide chain release factor PrfB2, chloroplastic isoform X1 [Benincasa hispida] >XP_038878323.1 peptide chain release factor PrfB2, chloroplastic isoform X1 [Benincasa hispida] >XP_038878325.1 peptide chain release factor PrfB2, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 105.1 bits (261), Expect = 3.3e-19
Identity = 62/91 (68.13%), Postives = 67/91 (73.63%), Query Frame = 0


Query: 1   MMRLELLSVELEKADLWDDATHAGKISREHGSIMDKIKEIKAFERELLEHIDMIKLASEG 60
           MMRLE LSVEL KADLWDD  HAGKISREHGSIM KIKEIKAFERELLEHIDMIKLA E 
Sbjct: 124 MMRLEFLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIKAFERELLEHIDMIKLAREE 183

Query: 61  ERSILKNPDLENSSIH-LSTTHKSGKTLERE 91
                 + +LE  SI+ L T  ++ K  E E
Sbjct: 184 -----NDTELELESINALLTMRRNSKEKELE 209

[Close]

BLAST of Spg023391 vs. NCBI nr
Match: XP_022989394.1 (peptide chain release factor PrfB2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 104.4 bits (259), Expect = 5.6e-19
Identity = 60/91 (65.93%), Postives = 69/91 (75.82%), Query Frame = 0


Query: 1   MMRLELLSVELEKADLWDDATHAGKISREHGSIMDKIKEIKAFERELLEHIDMIKLASEG 60
           MMRLELLSVEL KADLWDD  HAGKISREHGSIM KIKE+KAFERELLEHIDMIKLA E 
Sbjct: 129 MMRLELLSVELNKADLWDDPAHAGKISREHGSIMGKIKEMKAFERELLEHIDMIKLAREE 188

Query: 61  ERSILKNPDLENSSIH-LSTTHKSGKTLERE 91
                 + +LE+ S++ L +  ++ K  E E
Sbjct: 189 -----NDAELESESLNALLSMRRNSKEKELE 214

[Close]

BLAST of Spg023391 vs. NCBI nr
Match: XP_004135582.1 (peptide chain release factor PrfB2, chloroplastic [Cucumis sativus] >XP_011659591.1 peptide chain release factor PrfB2, chloroplastic [Cucumis sativus] >XP_011659596.1 peptide chain release factor PrfB2, chloroplastic [Cucumis sativus] >XP_031738430.1 peptide chain release factor PrfB2, chloroplastic [Cucumis sativus] >KGN66015.1 hypothetical protein Csa_007463 [Cucumis sativus])

HSP 1 Score: 103.6 bits (257), Expect = 9.6e-19
Identity = 61/95 (64.21%), Postives = 68/95 (71.58%), Query Frame = 0


Query: 1   MMRLELLSVELEKADLWDDATHAGKISREHGSIMDKIKEIKAFERELLEHIDMIKLASEG 60
           MMRLELLSVEL KADLWDD  HAGKISREHGSIM KIKEI AFERELLEHIDMIKLA E 
Sbjct: 124 MMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLAREE 183

Query: 61  ERSILKNPDLENSSIHLSTTHKSGKTLERETETES 96
                 + +LE+ S++   T +      RE E E+
Sbjct: 184 -----NDTELESESLNALLTMRRN---SREKELEA 210

[Close]

BLAST of Spg023391 vs. ExPASy Swiss-Prot
Match: F4I532 (Peptide chain release factor PrfB2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PRFB2 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.1e-17
Identity = 44/71 (61.97%), Postives = 56/71 (78.87%), Query Frame = 0


Query: 1   MMRLELLSVELEKADLWDDATHAGKISREHGSIMDKIKEIKAFERELLEHIDMIKLASEG 60
           ++RL++LS EL K+DLWDD THAGKISREHGS+  K+K +  FERELLEHIDM+KLA E 
Sbjct: 139 LVRLKVLSAELNKSDLWDDPTHAGKISREHGSLTGKMKGVMTFERELLEHIDMLKLAKEE 198

Query: 61  ERSILKNPDLE 72
             S L++  L+
Sbjct: 199 NDSELESETLK 209

[Close]

BLAST of Spg023391 vs. ExPASy Swiss-Prot
Match: Q2W3F8 (Peptide chain release factor 2 OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=prfB PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.1e-04
Identity = 25/70 (35.71%), Postives = 43/70 (61.43%), Query Frame = 0


Query: 1  MMRLELLSVELEKADLWDDATHAGKISREHGSIMDKIKEIKAFERELLEHIDMIKLAS-E 60
          +MRL+ L+   E  DLW+DA  A KI RE   +   I+  +A EREL +  ++I+L   E
Sbjct: 29 LMRLDELNASAENPDLWNDAGAAQKIMRERNELDSAIQGCRALERELADLAELIELGEME 88

Query: 61 GERSILKNPD 70
          G+++++ + +
Sbjct: 89 GDQTVIDDAE 98

[Close]

BLAST of Spg023391 vs. ExPASy TrEMBL
Match: A0A6J1EUC0 (peptide chain release factor PrfB2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111437810 PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.2e-19
Identity = 61/91 (67.03%), Postives = 69/91 (75.82%), Query Frame = 0


Query: 1   MMRLELLSVELEKADLWDDATHAGKISREHGSIMDKIKEIKAFERELLEHIDMIKLASEG 60
           MMRLELLSVEL KADLWDD  HAGKISREHGSIM KIKEIKAFERELLEHIDMIKLA E 
Sbjct: 129 MMRLELLSVELNKADLWDDPAHAGKISREHGSIMGKIKEIKAFERELLEHIDMIKLAREE 188

Query: 61  ERSILKNPDLENSSIH-LSTTHKSGKTLERE 91
                 + +LE+ S++ L +  ++ K  E E
Sbjct: 189 -----NDAELESESLNALLSMRRNSKEKELE 214

[Close]

BLAST of Spg023391 vs. ExPASy TrEMBL
Match: A0A6J1JP75 (peptide chain release factor PrfB2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111486461 PE=3 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.7e-19
Identity = 60/91 (65.93%), Postives = 69/91 (75.82%), Query Frame = 0


Query: 1   MMRLELLSVELEKADLWDDATHAGKISREHGSIMDKIKEIKAFERELLEHIDMIKLASEG 60
           MMRLELLSVEL KADLWDD  HAGKISREHGSIM KIKE+KAFERELLEHIDMIKLA E 
Sbjct: 129 MMRLELLSVELNKADLWDDPAHAGKISREHGSIMGKIKEMKAFERELLEHIDMIKLAREE 188

Query: 61  ERSILKNPDLENSSIH-LSTTHKSGKTLERE 91
                 + +LE+ S++ L +  ++ K  E E
Sbjct: 189 -----NDAELESESLNALLSMRRNSKEKELE 214

[Close]

BLAST of Spg023391 vs. ExPASy TrEMBL
Match: A0A1S4DY60 (peptide chain release factor PrfB2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492513 PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 4.6e-19
Identity = 61/95 (64.21%), Postives = 68/95 (71.58%), Query Frame = 0


Query: 1   MMRLELLSVELEKADLWDDATHAGKISREHGSIMDKIKEIKAFERELLEHIDMIKLASEG 60
           MMRLELLSVEL KADLWDD  HAGKISREHGSIM KIKEI AFERELLEHIDMIKLA E 
Sbjct: 124 MMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLAREE 183

Query: 61  ERSILKNPDLENSSIHLSTTHKSGKTLERETETES 96
                 + +LE+ S++   T +      RE E E+
Sbjct: 184 -----NDTELESESLNALLTMRRN---SREKELEA 210

[Close]

BLAST of Spg023391 vs. ExPASy TrEMBL
Match: A0A5D3CEK4 (Peptide chain release factor PrfB2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004440 PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 4.6e-19
Identity = 61/95 (64.21%), Postives = 68/95 (71.58%), Query Frame = 0


Query: 1   MMRLELLSVELEKADLWDDATHAGKISREHGSIMDKIKEIKAFERELLEHIDMIKLASEG 60
           MMRLELLSVEL KADLWDD  HAGKISREHGSIM KIKEI AFERELLEHIDMIKLA E 
Sbjct: 124 MMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLAREE 183

Query: 61  ERSILKNPDLENSSIHLSTTHKSGKTLERETETES 96
                 + +LE+ S++   T +      RE E E+
Sbjct: 184 -----NDTELESESLNALLTMRRN---SREKELEA 210

[Close]

BLAST of Spg023391 vs. ExPASy TrEMBL
Match: A0A0A0LVX3 (RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G561950 PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 4.6e-19
Identity = 61/95 (64.21%), Postives = 68/95 (71.58%), Query Frame = 0


Query: 1   MMRLELLSVELEKADLWDDATHAGKISREHGSIMDKIKEIKAFERELLEHIDMIKLASEG 60
           MMRLELLSVEL KADLWDD  HAGKISREHGSIM KIKEI AFERELLEHIDMIKLA E 
Sbjct: 124 MMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLAREE 183

Query: 61  ERSILKNPDLENSSIHLSTTHKSGKTLERETETES 96
                 + +LE+ S++   T +      RE E E+
Sbjct: 184 -----NDTELESESLNALLTMRRN---SREKELEA 210

[Close]

BLAST of Spg023391 vs. TAIR 10
Match: AT1G56350.1 (Peptide chain release factor 2 )

HSP 1 Score: 90.5 bits (223), Expect = 7.8e-19
Identity = 44/71 (61.97%), Postives = 56/71 (78.87%), Query Frame = 0


Query: 1   MMRLELLSVELEKADLWDDATHAGKISREHGSIMDKIKEIKAFERELLEHIDMIKLASEG 60
           ++RL++LS EL K+DLWDD THAGKISREHGS+  K+K +  FERELLEHIDM+KLA E 
Sbjct: 139 LVRLKVLSAELNKSDLWDDPTHAGKISREHGSLTGKMKGVMTFERELLEHIDMLKLAKEE 198

Query: 61  ERSILKNPDLE 72
             S L++  L+
Sbjct: 199 NDSELESETLK 209

The following BLAST results are available for this feature:

BLAST of Spg023391 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023531900.1	2.5e-19	67.03	peptide chain release factor PrfB2, chloroplastic [Cucurbita pepo subsp. pepo] >...	[more]
XP_022931672.1	2.5e-19	67.03	peptide chain release factor PrfB2, chloroplastic [Cucurbita moschata]	[more]
XP_038878322.1	3.3e-19	68.13	peptide chain release factor PrfB2, chloroplastic isoform X1 [Benincasa hispida]...	[more]
XP_022989394.1	5.6e-19	65.93	peptide chain release factor PrfB2, chloroplastic [Cucurbita maxima]	[more]
XP_004135582.1	9.6e-19	64.21	peptide chain release factor PrfB2, chloroplastic [Cucumis sativus] >XP_01165959...	[more]

BLAST of Spg023391 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I532	1.1e-17	61.97	Peptide chain release factor PrfB2, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q2W3F8	1.1e-04	35.71	Peptide chain release factor 2 OS=Magnetospirillum magneticum (strain AMB-1 / AT...	[more]

BLAST of Spg023391 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EUC0	1.2e-19	67.03	peptide chain release factor PrfB2, chloroplastic OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1JP75	2.7e-19	65.93	peptide chain release factor PrfB2, chloroplastic OS=Cucurbita maxima OX=3661 GN...	[more]
A0A1S4DY60	4.6e-19	64.21	peptide chain release factor PrfB2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A5D3CEK4	4.6e-19	64.21	Peptide chain release factor PrfB2 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A0A0LVX3	4.6e-19	64.21	RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G561950 P...	[more]

BLAST of Spg023391 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56350.1	7.8e-19	61.97	Peptide chain release factor 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.58.410	Release factor	coord: 1..93 e-value: 4.7E-9 score: 38.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 74..95
None	No IPR available	SUPERFAMILY	75620	Release factor	coord: 3..61

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg023391.1	Spg023391.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006415	translational termination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016149	translation release factor activity, codon specific