Spg023020 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg023020
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Gluconokinase
Location	scaffold12: 28870502 .. 28874008 (-)
RNA-Seq Expression	Spg023020
Synteny	Spg023020

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCTCTCAGCATCCGATTTCGAACGGTATTCTATTCTATTCTTCAATTTCTCTCTTACGCTTCTCAATTTCATTCATTCATTCATTGAATTCTGACATTAATCTGGTTTCAAGCTTTGGCGATCGTGCTTATGGGCGTCAGCGGTTCTGGAAAATCGTAAGTCTCTTCTCTCTCCGATCTACGGCCGATTCTTCGTCTTCTGTAAATAAAACGATTGATCGATTCAACGGCTTTAGATGATATGAAATTCAATTTAGAGTTGAAATATTGGAGAAAATATATTTAACCTGAGATGAATTTGTGTTACTGAATTCCTTATTGTGGAATTTTCATTTCTGGGTTGATTCTGTTTTCTGTGATCGAAGGGAATTCGTTTTCCCCCTTCCGATGATTTTGATGAGTCGTGTGTGTGTGTGGTGCAGTACTATTGGTGCGATGCTGGCCAACTCCATGGACTTCTTCACTTTTCTTGATGCTGATGATTTTCACCCAATTTCTAACAAGGGTATGCCGCAACCTCTTTCTCTAGCTTGTTTGTTTTATTTAGGGTTTAAATCCTCCTTAAACTTAAATATATCTTTTCGGCACTTTTGGTCCCTCAACCTATGAAAGGTAACAAAATTTAACAGTTTAATATTTTCTACACTTTAAAATTTGTAATAGTTAATGTTTAAAAAATCCCATCAAAATTAAGTTTGAACACATTCAATTTATTGATATAGGTAAGAAATTTCATTAAAACATAACAATCTTTTTCATGATAAAAGACTAAATTCGTTATAAATTTTAAAATATGACTAAATCTTTATACACTAAAGTTCATGATTAAATTGTTACAAAATTCAAAGTATGAAACTAAATTGTTACAAACGAAAATCCAAGGATGTAAGTTCAAAGACCAAAGTGGTTTTTAACCATCATGGGTTGGCCCTAATGGTCAATAAGGGCCATAAAAAAAATAAAGGGCTTAGAGAGAATGGGTTGACCTACCTAGGATATTTAATATCCTACGAGTTTCCTTGGCAACCAAATGTAGTAGGGTCAGGCGGTTGTTCCGTGAGATTAGTCGAGGTGCGCGTAAGTTGGCCCGGACACTCACGGATATCAAAAAAAAAAAAAAAAAAAAAAGACCAAAGTGGTTTTTAGCCTATTTCTTTCCAGCTTAGTATCTATTGTTTAAAGAATTTATATTTAGCCGTCCCTTATATATTAACATTATTCAATTTAGTCCATAATTATTGGTGAGTGTTAAAATTGGTTGATAAACTTAGATGACATAATACTCGGTGAATTGACATAAATTTAGTGGAGAAAAAATGTTATCTAGGAGGAAAATTTTGAATTAATAATTTTTTAGAAATTTGGAGGAATTAGAAATTTTCATCTTTTACATAACTTTTTATTCCTTTTCTAAGGGTTAGTATGAAATACCTTTTAGTTTAAATGTTTTTCAAGAATGCATTTTATTTTAAACATTTTGTATAAAAATTGTTTAGAATTTAAACATTCATATGTTTGATTACATTTCTTCATAAGTGTTTTTATACACAAGTTATTATATATATATTTTTAAAAAATTGATTTCAAGTGTTTCTCTCGAAATGACTTATTTCACAATCTCATTTTTTAATAAGTCATTTTTAATAGTTGCCAAACACTAAAAAAAAAAATTCAAAATGATTTTTTCTTCAAAATTAATTAAACACTTGAAAAGTTAAACCAAACATACTCTAAATTTCTACTCATCCACTAAATGTAACTTTGTGGTAAACTAATTCTTAACATTCACCACCATTAAGAGATTAGTTAAATTGAAACATTTTTTAATAATAGGGACTAAATTTTAAAAAGAACGTCCACACATCTGAGACTAAAACATGTATTTTTCTTTTTAAAAAAGAGACTAAAATATGTATTTAGCCCTTTAAACTTATTAGCATTCTATTTCTTTTCCAAAAATTTTGAATCGTTTTATTTAATTTTTGAATTTTGTATAAAAAATTATTTTAATTCTTGGGTTTATTATTTTAATTATTTAATAAAAATTTGACAAAAACACTTATTTTCTTGTTTGTAATAGAAGTTGAGTTTTGAAATATATTTAATTATTTGTTAAATGGTCAACAAAAAATTAACTCAAGGAACCTAAATATAGCTTTTTTTTTCCTTGCAAAGTTTAGAGCAGAAAACAAAATAATTGAAAGTTAGGGAGATTGAAAATAAAATATTTACATATTTTAGAACCAAAATATAATGCTACGAATGTTTAGAACTTGTACCAATTTATCCATGTTGCAAAGTATCACGAGGGATGATGACCAAGGGTGATCATGACACATAACTTTAAAGAAAATAAAAAGTAAGCTAAATAAATATTTGAGTCCTCAATGTCTAGGATTTTTACAATTTAGTCTCCAATGTTTAAAAAGATTTGATTTAGTTCTTTATGTACATTTTTCAATTTAATCATTGACCGATTGTTAAAATTGACTAATGATAAACTTACCTTTCATAATACTTAGTGAATTGTTGGAAATTTAGGGGGAAATGATTATAAGAGAGAAAAGTTTCTAATTCCTTCAAATTTCTAAGAAATGAGTAGATATTATGATGAGGACCCACTTGAAGAATGTTAATGCATAAGGTACTATGTTGAAACTTGTTAAACAATATACTTGTAAAAAAATTGTAAAATTACTCAAACATTAGAAGTAAAAAAATATATGGAACAAATAGAAAGTATTTATCAAAATTTTTATAGAATTTGGCTTCTTTGTTAAAAATTTTAAAATATTTAAAGCATAGTCCTTTTTCTCAATAGCACTCTGAGTAAACAAGTATTTCACATAAAGTACTACTTTCAAAAGTCATTTCAAACCCACCTCGAAACTTTCATTGTCATGAACAGAAAAAATGTCGAAAGGAATCCCTCTTTCGGACGAAGATCGGATCCCCTGGCTCGAGAAGCTTCGAGACACCTTGAGAGAGAACATAGGTTGTAGAAGCAGAGTAATTCTTGGTTGCTCAGCTCTAAGAAAACAGTACAGAGAGACTCTGAGATCAGCAGATCCAAATTATAAAATGGGAATGAGCTGTGTGGTGAAGTTTGTTCTGTTGGATGCTCCAGCTGAAGTGATTGCTTCCAGATTGGAGAAAAGAGCCAAAGAAGGGAAGCATTTCATGCCTTCAACTCTTTTGAAATCCCAATTGGATCTGTTGCAGATTGATGATTCTGAAGGCATTTTAAAAGTTGATGCTACTCTTAGCCCTCAAGTAATTGTGAGTAATATTCTTAAGTTGCTTTTTGGAACTAACAACAATGGGAGATGATCATATATAGTTGATTTTCTCATCCTCTTTTTCTTGTTTTCACTTTCTCTTTAAAACTTAGTAGTCATGTGATTTTCTGGTGCTCCTAATGGAAACATTATTGTCAAGGACATTTTTGAAACAGTTTGAAATGTAAATAAAAATTGAAACTTTTGAAACGATAACAGTAATTTT

mRNA sequence

ATGGGCTCTCAGCATCCGATTTCGAACGGTATTCTATTCTATTCTTCAATTTCTCTCTTACGCTTCTCAATTTCATTCATTCATTCATTGAATTCTGACATTAATCTGGTTTCAAGCTTTGGCGATCGTGCTTATGGGCGTCAGCGTACTATTGGTGCGATGCTGGCCAACTCCATGGACTTCTTCACTTTTCTTGATGCTGATGATTTTCACCCAATTTCTAACAAGGAAAAAATGTCGAAAGGAATCCCTCTTTCGGACGAAGATCGGATCCCCTGGCTCGAGAAGCTTCGAGACACCTTGAGAGAGAACATAGGTTGTAGAAGCAGAGTAATTCTTGGTTGCTCAGCTCTAAGAAAACAGTACAGAGAGACTCTGAGATCAGCAGATCCAAATTATAAAATGGGAATGAGCTGTGTGGTGAAGTTTGTTCTGTTGGATGCTCCAGCTGAAGTGATTGCTTCCAGATTGGAGAAAAGAGCCAAAGAAGGGAAGCATTTCATGCCTTCAACTCTTTTGAAATCCCAATTGGATCTGTTGCAGATTGATGATTCTGAAGGCATTTTAAAAGTTGATGCTACTCTTAGCCCTCAAGTAATTGTGAGTAATATTCTTAAGTTGCTTTTTGGAACTAACAACAATGGGAGATGA

Coding sequence (CDS)

ATGGGCTCTCAGCATCCGATTTCGAACGGTATTCTATTCTATTCTTCAATTTCTCTCTTACGCTTCTCAATTTCATTCATTCATTCATTGAATTCTGACATTAATCTGGTTTCAAGCTTTGGCGATCGTGCTTATGGGCGTCAGCGTACTATTGGTGCGATGCTGGCCAACTCCATGGACTTCTTCACTTTTCTTGATGCTGATGATTTTCACCCAATTTCTAACAAGGAAAAAATGTCGAAAGGAATCCCTCTTTCGGACGAAGATCGGATCCCCTGGCTCGAGAAGCTTCGAGACACCTTGAGAGAGAACATAGGTTGTAGAAGCAGAGTAATTCTTGGTTGCTCAGCTCTAAGAAAACAGTACAGAGAGACTCTGAGATCAGCAGATCCAAATTATAAAATGGGAATGAGCTGTGTGGTGAAGTTTGTTCTGTTGGATGCTCCAGCTGAAGTGATTGCTTCCAGATTGGAGAAAAGAGCCAAAGAAGGGAAGCATTTCATGCCTTCAACTCTTTTGAAATCCCAATTGGATCTGTTGCAGATTGATGATTCTGAAGGCATTTTAAAAGTTGATGCTACTCTTAGCCCTCAAGTAATTGTGAGTAATATTCTTAAGTTGCTTTTTGGAACTAACAACAATGGGAGATGA

Protein sequence

MGSQHPISNGILFYSSISLLRFSISFIHSLNSDINLVSSFGDRAYGRQRTIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKLRDTLRENIGCRSRVILGCSALRKQYRETLRSADPNYKMGMSCVVKFVLLDAPAEVIASRLEKRAKEGKHFMPSTLLKSQLDLLQIDDSEGILKVDATLSPQVIVSNILKLLFGTNNNGR

Homology

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BLAST of Spg023020 vs. NCBI nr
Match: XP_038874456.1 (gluconokinase [Benincasa hispida] >XP_038874457.1 gluconokinase [Benincasa hispida])

HSP 1 Score: 264.2 bits (674), Expect = 9.6e-67
Identity = 137/170 (80.59%), Postives = 148/170 (87.06%), Query Frame = 0


Query: 50  TIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKLRDTLRENIGCRS 109
           TIG ML  ++D FTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEK+RDTLRENI CR 
Sbjct: 15  TIGEMLGKALD-FTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKIRDTLRENITCRR 74

Query: 110 RVILGCSALRKQYRETLRSADPNYKM--GMSCVVKFVLLDAPAEVIASRLEKRAKEGKHF 169
            V+LGCSAL+K YRE LRS+D NY+    M CVVKFVLLDAPAEVIASRLEKRAKEG HF
Sbjct: 75  SVVLGCSALQKHYREILRSSDQNYEKMETMKCVVKFVLLDAPAEVIASRLEKRAKEGNHF 134

Query: 170 MPSTLLKSQLDLLQIDDSEGILKVDATLSPQVIVSNILKLLFG----TNN 214
           MPSTLLKSQLDLLQIDDSEGI+KVDATL+PQ IVSNI+ L+FG    TNN
Sbjct: 135 MPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLVFGSLVLTNN 183

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BLAST of Spg023020 vs. NCBI nr
Match: XP_022995367.1 (gluconokinase [Cucurbita maxima])

HSP 1 Score: 253.8 bits (647), Expect = 1.3e-63
Identity = 131/162 (80.86%), Postives = 145/162 (89.51%), Query Frame = 0


Query: 50  TIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKLRDTLRENIGCRS 109
           TIGAMLA +M   TFLDADDFHP SNKEKMSKGIPLSDEDRIPWLEK+R TLRE +G ++
Sbjct: 25  TIGAMLAEAMG-LTFLDADDFHPSSNKEKMSKGIPLSDEDRIPWLEKIRGTLREKMGSKN 84

Query: 110 RVILGCSALRKQYRETLRSADPNYK-MGMSCVVKFVLLDAPAEVIASRLEKRAKEGKHFM 169
            V+LGCSAL+KQYRE LRSADPNY+ +G+SCVVKFVLLDAPAEVIA RLEKRAKEG HFM
Sbjct: 85  SVVLGCSALQKQYREILRSADPNYEGIGISCVVKFVLLDAPAEVIALRLEKRAKEGNHFM 144

Query: 170 PSTLLKSQLDLLQIDDSEGILKVDATLSPQVIVSNILKLLFG 211
           PSTLL SQLDLLQID SEGIL+VDATLSPQ IVS+I+KL+ G
Sbjct: 145 PSTLLNSQLDLLQIDVSEGILQVDATLSPQAIVSSIIKLVRG 185

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BLAST of Spg023020 vs. NCBI nr
Match: XP_023532636.1 (gluconokinase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 252.7 bits (644), Expect = 2.9e-63
Identity = 130/162 (80.25%), Postives = 143/162 (88.27%), Query Frame = 0


Query: 50  TIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKLRDTLRENIGCRS 109
           TIGAMLA +M   TFLDADDFHP SNKEKMSKGIPLS+EDRIPWLEK+RDTLRE +G + 
Sbjct: 25  TIGAMLAEAMG-LTFLDADDFHPSSNKEKMSKGIPLSEEDRIPWLEKIRDTLREKMGSKK 84

Query: 110 RVILGCSALRKQYRETLRSADPNYK-MGMSCVVKFVLLDAPAEVIASRLEKRAKEGKHFM 169
            V+LGCSAL+KQYR+ LRSADPNY+ +G+ CVVKFVLLDAPAEVIA RLEKRAKEG HFM
Sbjct: 85  SVVLGCSALQKQYRDILRSADPNYEGIGIRCVVKFVLLDAPAEVIAFRLEKRAKEGNHFM 144

Query: 170 PSTLLKSQLDLLQIDDSEGILKVDATLSPQVIVSNILKLLFG 211
           PSTLL SQLDLLQID SEGILKVDATLSPQ IVS I+KL+ G
Sbjct: 145 PSTLLNSQLDLLQIDVSEGILKVDATLSPQAIVSRIIKLVRG 185

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BLAST of Spg023020 vs. NCBI nr
Match: XP_004143662.1 (gluconokinase isoform X1 [Cucumis sativus])

HSP 1 Score: 252.3 bits (643), Expect = 3.8e-63
Identity = 132/182 (72.53%), Postives = 152/182 (83.52%), Query Frame = 0


Query: 30  LNSDINLVSSFGDRAYGRQRTIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDED 89
           +NS+  ++   G    G+  TIG ML  ++D FTFLDAD FHPISNKEKMSKGIPLSDED
Sbjct: 1   MNSNAMVIILMGVCGSGKS-TIGEMLGKALD-FTFLDADHFHPISNKEKMSKGIPLSDED 60

Query: 90  RIPWLEKLRDTLRENIGCRSRVILGCSALRKQYRETLRSADPNY-KMG--MSCVVKFVLL 149
           R+PWLEK+RDTLRENI C+S V+LGCSAL+K YRE LRS+DPNY K+G  M CVVKFVLL
Sbjct: 61  RMPWLEKIRDTLRENIACKSSVVLGCSALQKHYREILRSSDPNYEKVGITMKCVVKFVLL 120

Query: 150 DAPAEVIASRLEKRAKEGKHFMPSTLLKSQLDLLQIDDSEGILKVDATLSPQVIVSNILK 209
           DAPAEVIA RLEKRAKEG HFMPS LLKSQLDLLQI+D+EGI+KVDAT +PQ I+SNIL 
Sbjct: 121 DAPAEVIALRLEKRAKEGNHFMPSALLKSQLDLLQIEDTEGIVKVDATQTPQAILSNILN 180

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BLAST of Spg023020 vs. NCBI nr
Match: XP_022159526.1 (uncharacterized protein LOC111025918 isoform X1 [Momordica charantia])

HSP 1 Score: 251.9 bits (642), Expect = 5.0e-63
Identity = 126/162 (77.78%), Postives = 146/162 (90.12%), Query Frame = 0


Query: 50  TIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKLRDTLRENIGCRS 109
           TIG MLA +M+   FLDADDFHP SNKEKMSKGIPLS+EDR+PWLEK+RD L+E+I C+ 
Sbjct: 26  TIGEMLAKAMN-LNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKVRDALKESIACKK 85

Query: 110 RVILGCSALRKQYRETLRSADPNYKMGMSCVVKFVLLDAPAEVIASRLEKRAKEGKHFMP 169
            V+LGCSAL+KQYRE LRSADPNY++G SCVVKFVLLDAP EVIA RLE+RAKEG HFM 
Sbjct: 86  SVVLGCSALQKQYREILRSADPNYEIG-SCVVKFVLLDAPPEVIAYRLERRAKEGNHFMS 145

Query: 170 STLLKSQLDLLQIDDSEGILKVDATLSPQVIVSNILKLLFGT 212
           STLL+SQLDLL+IDDSEGILKVDATL+PQVIV+NILK++FG+
Sbjct: 146 STLLESQLDLLEIDDSEGILKVDATLTPQVIVTNILKVVFGS 185

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BLAST of Spg023020 vs. ExPASy Swiss-Prot
Match: Q9SLE0 (Gluconokinase OS=Arabidopsis thaliana OX=3702 GN=At2g16790 PE=2 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 1.7e-45
Identity = 94/166 (56.63%), Postives = 124/166 (74.70%), Query Frame = 0


Query: 50  TIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKLRDTLRENIGCRS 109
           TIG ML  ++    FLDADDFH +SN++KM +GI LSDEDR+PWLEK++++LR+ +    
Sbjct: 24  TIGKMLGKALS-CDFLDADDFHSLSNRDKMRQGIALSDEDRMPWLEKIQESLRKRLLDGE 83

Query: 110 RVILGCSALRKQYRETLRSADPNYKMG--MSCVVKFVLLDAPAEVIASRLEKRAKEGKHF 169
            V+L CS+LRKQYRE LR +DP+YK G   SC V FVLL+  AEVIA+RL+KRA E +HF
Sbjct: 84  TVVLACSSLRKQYREILRGSDPDYKPGSYTSCKVTFVLLEGNAEVIAARLQKRASEEEHF 143

Query: 170 MPSTLLKSQLDLLQIDDSEGILKVDATLSPQVIVSNILKLLFGTNN 214
           MP TLL+SQ DLLQ D+ E I K+   LSP+VIV+ IL+++  + N
Sbjct: 144 MPLTLLQSQFDLLQADECEKIFKISVVLSPEVIVNTILEMVANSLN 188

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BLAST of Spg023020 vs. ExPASy Swiss-Prot
Match: Q5T6J7 (Probable gluconokinase OS=Homo sapiens OX=9606 GN=IDNK PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 4.4e-22
Identity = 66/172 (38.37%), Postives = 96/172 (55.81%), Query Frame = 0


Query: 50  TIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKLRDTLRENIGCRS 109
           T+GA+LA+ +  + F DADD+HP  N+ KM KGIPL+D+DRIPWL  L D L  ++    
Sbjct: 19  TVGALLASELG-WKFYDADDYHPEENRRKMGKGIPLNDQDRIPWLCNLHDILLRDVASGQ 78

Query: 110 RVILGCSALRKQYRETLRSADPNYKMGMSC------------VVKFVLLDAPAEVIASRL 169
           RV+L CSAL+K YR+ L        + + C             +  V L    EVI+ RL
Sbjct: 79  RVVLACSALKKTYRDILTQGKDG--VALKCEESGKEAKQAEMQLLVVHLSGSFEVISGRL 138

Query: 170 EKRAKEGKHFMPSTLLKSQLDLLQIDDS-EGILKVDATLSPQVIVSNILKLL 209
            KR  EG HFMP  LL+SQ + L+   + E  +++    +   I++ I++ L
Sbjct: 139 LKR--EG-HFMPPELLQSQFETLEPPAAPENFIQISVDKNVSEIIATIMETL 184

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BLAST of Spg023020 vs. ExPASy Swiss-Prot
Match: B0BML1 (Probable gluconokinase OS=Xenopus tropicalis OX=8364 GN=idnk PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 5.8e-22
Identity = 64/162 (39.51%), Postives = 91/162 (56.17%), Query Frame = 0


Query: 51  IGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKLRDTLRENIGCRSR 110
           +G+ LA  +  + F DADD+HP+ NKEKMS+G PL+D+DR PWL +L + +         
Sbjct: 16  VGSQLAKKLG-WNFYDADDYHPLENKEKMSQGTPLNDQDRHPWLCELHEIMMREKALGQH 75

Query: 111 VILGCSALRKQYRETLRSAD-----PNYKMG--MSCVVKFVLLDAPAEVIASRLEKRAKE 170
           V+L CSAL++ YR TL +        NY+    +S    FV L    E+++ RL +R   
Sbjct: 76  VVLACSALKRAYRSTLLTGSTPHWPENYQENDDLSSDTLFVHLHGSLEILSRRLLERK-- 135

Query: 171 GKHFMPSTLLKSQLDLLQIDDS-EGILKVDATLSPQVIVSNI 205
             HFMP TLL SQ+D L+   + E  + +D      VIVS I
Sbjct: 136 -GHFMPRTLLDSQIDTLEPPSAPERFIAIDVDKDISVIVSEI 173

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BLAST of Spg023020 vs. ExPASy Swiss-Prot
Match: P39208 (Thermosensitive gluconokinase OS=Escherichia coli (strain K12) OX=83333 GN=idnK PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 9.8e-22
Identity = 57/131 (43.51%), Postives = 78/131 (59.54%), Query Frame = 0


Query: 64  FLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKLRDTLRENIGCRSRVILGCSALRKQYR 123
           F+D DD HP  N +KMS+GIPLSDEDR+PWLE+L D             + CS+L+KQYR
Sbjct: 31  FIDGDDLHPAKNIDKMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYR 90

Query: 124 ETLRSADPNYKMGMSCVVKFVLLDAPAEVIASRLEKRAKEGKHFMPSTLLKSQLDLLQID 183
           + LR   P+        V F+ LD   E I +R+++RA    HFMP  LLKSQ + L+  
Sbjct: 91  DILRKGSPH--------VHFLWLDGDYETILARMQRRA---GHFMPVALLKSQFEALERP 150

Query: 184 --DSEGILKVD 193
             D + I+++D
Sbjct: 151 QADEQDIVRID 150

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BLAST of Spg023020 vs. ExPASy Swiss-Prot
Match: Q5FQ97 (Gluconokinase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=GOX1709 PE=1 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 1.4e-20
Identity = 60/158 (37.97%), Postives = 88/158 (55.70%), Query Frame = 0


Query: 50  TIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKLRDTLRENIGCRS 109
           T+   LA  + +  F + D  HP +N EKMS G PL+D DR PWL    D LRE +    
Sbjct: 27  TVATGLATRLGWH-FQEGDALHPPANVEKMSTGQPLTDADRAPWLALCHDWLREQVKAGH 86

Query: 110 RVILGCSALRKQYRETLRSADPNYKMGMSCVVKFVLLDAPAEVIASRLEKRAKEGKHFMP 169
             +L CSAL++ YRE LR  D          ++FV +D     +A RL++R  EG HFMP
Sbjct: 87  GAVLTCSALKRSYREQLRGDD--------LPIEFVHIDTSTGELADRLQRR--EG-HFMP 146

Query: 170 STLLKSQLDLLQI-DDSEGILKVDATLSPQVIVSNILK 207
           ++LL SQL  L++  D E +++V     P V++  +++
Sbjct: 147 ASLLPSQLATLEVPGDDEPVIRVSGEKHPDVVLEELIR 172

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BLAST of Spg023020 vs. ExPASy TrEMBL
Match: A0A6J1JYN8 (Gluconokinase OS=Cucurbita maxima OX=3661 GN=LOC111490938 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 6.3e-64
Identity = 131/162 (80.86%), Postives = 145/162 (89.51%), Query Frame = 0


Query: 50  TIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKLRDTLRENIGCRS 109
           TIGAMLA +M   TFLDADDFHP SNKEKMSKGIPLSDEDRIPWLEK+R TLRE +G ++
Sbjct: 25  TIGAMLAEAMG-LTFLDADDFHPSSNKEKMSKGIPLSDEDRIPWLEKIRGTLREKMGSKN 84

Query: 110 RVILGCSALRKQYRETLRSADPNYK-MGMSCVVKFVLLDAPAEVIASRLEKRAKEGKHFM 169
            V+LGCSAL+KQYRE LRSADPNY+ +G+SCVVKFVLLDAPAEVIA RLEKRAKEG HFM
Sbjct: 85  SVVLGCSALQKQYREILRSADPNYEGIGISCVVKFVLLDAPAEVIALRLEKRAKEGNHFM 144

Query: 170 PSTLLKSQLDLLQIDDSEGILKVDATLSPQVIVSNILKLLFG 211
           PSTLL SQLDLLQID SEGIL+VDATLSPQ IVS+I+KL+ G
Sbjct: 145 PSTLLNSQLDLLQIDVSEGILQVDATLSPQAIVSSIIKLVRG 185

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BLAST of Spg023020 vs. ExPASy TrEMBL
Match: A0A0A0KN86 (Gluconokinase OS=Cucumis sativus OX=3659 GN=Csa_5G169050 PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.8e-63
Identity = 132/182 (72.53%), Postives = 152/182 (83.52%), Query Frame = 0


Query: 30  LNSDINLVSSFGDRAYGRQRTIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDED 89
           +NS+  ++   G    G+  TIG ML  ++D FTFLDAD FHPISNKEKMSKGIPLSDED
Sbjct: 1   MNSNAMVIILMGVCGSGKS-TIGEMLGKALD-FTFLDADHFHPISNKEKMSKGIPLSDED 60

Query: 90  RIPWLEKLRDTLRENIGCRSRVILGCSALRKQYRETLRSADPNY-KMG--MSCVVKFVLL 149
           R+PWLEK+RDTLRENI C+S V+LGCSAL+K YRE LRS+DPNY K+G  M CVVKFVLL
Sbjct: 61  RMPWLEKIRDTLRENIACKSSVVLGCSALQKHYREILRSSDPNYEKVGITMKCVVKFVLL 120

Query: 150 DAPAEVIASRLEKRAKEGKHFMPSTLLKSQLDLLQIDDSEGILKVDATLSPQVIVSNILK 209
           DAPAEVIA RLEKRAKEG HFMPS LLKSQLDLLQI+D+EGI+KVDAT +PQ I+SNIL 
Sbjct: 121 DAPAEVIALRLEKRAKEGNHFMPSALLKSQLDLLQIEDTEGIVKVDATQTPQAILSNILN 180

[Close]

BLAST of Spg023020 vs. ExPASy TrEMBL
Match: A0A6J1E468 (Gluconokinase OS=Momordica charantia OX=3673 GN=LOC111025918 PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 2.4e-63
Identity = 126/162 (77.78%), Postives = 146/162 (90.12%), Query Frame = 0


Query: 50  TIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKLRDTLRENIGCRS 109
           TIG MLA +M+   FLDADDFHP SNKEKMSKGIPLS+EDR+PWLEK+RD L+E+I C+ 
Sbjct: 26  TIGEMLAKAMN-LNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKVRDALKESIACKK 85

Query: 110 RVILGCSALRKQYRETLRSADPNYKMGMSCVVKFVLLDAPAEVIASRLEKRAKEGKHFMP 169
            V+LGCSAL+KQYRE LRSADPNY++G SCVVKFVLLDAP EVIA RLE+RAKEG HFM 
Sbjct: 86  SVVLGCSALQKQYREILRSADPNYEIG-SCVVKFVLLDAPPEVIAYRLERRAKEGNHFMS 145

Query: 170 STLLKSQLDLLQIDDSEGILKVDATLSPQVIVSNILKLLFGT 212
           STLL+SQLDLL+IDDSEGILKVDATL+PQVIV+NILK++FG+
Sbjct: 146 STLLESQLDLLEIDDSEGILKVDATLTPQVIVTNILKVVFGS 185

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BLAST of Spg023020 vs. ExPASy TrEMBL
Match: A0A6J1H380 (Gluconokinase OS=Cucurbita moschata OX=3662 GN=LOC111459704 PE=3 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 5.3e-63
Identity = 129/162 (79.63%), Postives = 143/162 (88.27%), Query Frame = 0


Query: 50  TIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKLRDTLRENIGCRS 109
           TIGAMLA +M   TFLDADDFHP SNKEKMSKGIPLSDEDRIPWLEK+RDTLRE +G ++
Sbjct: 25  TIGAMLAEAMG-LTFLDADDFHPSSNKEKMSKGIPLSDEDRIPWLEKIRDTLREKMGSKN 84

Query: 110 RVILGCSALRKQYRETLRSADPNYK-MGMSCVVKFVLLDAPAEVIASRLEKRAKEGKHFM 169
            V+LGCSAL+KQYR+ LRSADPNY+ +G+ CVVKFVLLDAPAEVIA RLEKRAKEG HFM
Sbjct: 85  SVVLGCSALQKQYRDILRSADPNYEGIGIRCVVKFVLLDAPAEVIALRLEKRAKEGNHFM 144

Query: 170 PSTLLKSQLDLLQIDDSEGILKVDATLSPQVIVSNILKLLFG 211
           PSTLL SQLDLLQID SEGIL+VDAT SPQ IVS I+KL+ G
Sbjct: 145 PSTLLNSQLDLLQIDVSEGILQVDATRSPQAIVSRIIKLVRG 185

[Close]

BLAST of Spg023020 vs. ExPASy TrEMBL
Match: A0A1S3CTF0 (Gluconokinase OS=Cucumis melo OX=3656 GN=LOC103504681 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 1.2e-62
Identity = 132/182 (72.53%), Postives = 150/182 (82.42%), Query Frame = 0


Query: 30  LNSDINLVSSFGDRAYGRQRTIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDED 89
           +NS+  ++   G    G+  TIG ML  ++D FTFLDAD FHPISNKEKMSKGIPLSDED
Sbjct: 1   MNSNAMVIVLMGVCGSGKS-TIGEMLGKALD-FTFLDADHFHPISNKEKMSKGIPLSDED 60

Query: 90  RIPWLEKLRDTLRENIGCRSRVILGCSALRKQYRETLRSADPNY-KMG--MSCVVKFVLL 149
           R+PWLEK+RDTLRENI  +  V++GCSAL+K YRE LRS+DPNY KMG  M CVVKFVLL
Sbjct: 61  RMPWLEKIRDTLRENIASKRSVVVGCSALQKHYREILRSSDPNYEKMGITMKCVVKFVLL 120

Query: 150 DAPAEVIASRLEKRAKEGKHFMPSTLLKSQLDLLQIDDSEGILKVDATLSPQVIVSNILK 209
           DAPAEVIA RLEKRAKEG HFMPS LLKSQLDLLQIDDSEGI+KVDAT +PQ I+SNIL 
Sbjct: 121 DAPAEVIALRLEKRAKEGNHFMPSALLKSQLDLLQIDDSEGIVKVDATQTPQAILSNILN 180

[Close]

BLAST of Spg023020 vs. TAIR 10
Match: AT2G16790.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 186.0 bits (471), Expect = 3.1e-47
Identity = 98/187 (52.41%), Postives = 133/187 (71.12%), Query Frame = 0


Query: 29  SLNSDINLVSSFGDRAYGRQRTIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDE 88
           S  +D  +++  G    G+  TIG ML  ++    FLDADDFH +SN++KM +GI LSDE
Sbjct: 2   SAKNDGKVIAIMGVSGAGKS-TIGKMLGKALS-CDFLDADDFHSLSNRDKMRQGIALSDE 61

Query: 89  DRIPWLEKLRDTLRENIGCRSRVILGCSALRKQYRETLRSADPNYKMG--MSCVVKFVLL 148
           DR+PWLEK++++LR+ +     V+L CS+LRKQYRE LR +DP+YK G   SC V FVLL
Sbjct: 62  DRMPWLEKIQESLRKRLLDGETVVLACSSLRKQYREILRGSDPDYKPGSYTSCKVTFVLL 121

Query: 149 DAPAEVIASRLEKRAKEGKHFMPSTLLKSQLDLLQIDDSEGILKVDATLSPQVIVSNILK 208
           +  AEVIA+RL+KRA E +HFMP TLL+SQ DLLQ D+ E I K+   LSP+VIV+ IL+
Sbjct: 122 EGNAEVIAARLQKRASEEEHFMPLTLLQSQFDLLQADECEKIFKISVVLSPEVIVNTILE 181

Query: 209 LLFGTNN 214
           ++  + N
Sbjct: 182 MVANSLN 186

[Close]

BLAST of Spg023020 vs. TAIR 10
Match: AT2G16790.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 184.1 bits (466), Expect = 1.2e-46
Identity = 94/166 (56.63%), Postives = 124/166 (74.70%), Query Frame = 0


Query: 50  TIGAMLANSMDFFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKLRDTLRENIGCRS 109
           TIG ML  ++    FLDADDFH +SN++KM +GI LSDEDR+PWLEK++++LR+ +    
Sbjct: 24  TIGKMLGKALS-CDFLDADDFHSLSNRDKMRQGIALSDEDRMPWLEKIQESLRKRLLDGE 83

Query: 110 RVILGCSALRKQYRETLRSADPNYKMG--MSCVVKFVLLDAPAEVIASRLEKRAKEGKHF 169
            V+L CS+LRKQYRE LR +DP+YK G   SC V FVLL+  AEVIA+RL+KRA E +HF
Sbjct: 84  TVVLACSSLRKQYREILRGSDPDYKPGSYTSCKVTFVLLEGNAEVIAARLQKRASEEEHF 143

Query: 170 MPSTLLKSQLDLLQIDDSEGILKVDATLSPQVIVSNILKLLFGTNN 214
           MP TLL+SQ DLLQ D+ E I K+   LSP+VIV+ IL+++  + N
Sbjct: 144 MPLTLLQSQFDLLQADECEKIFKISVVLSPEVIVNTILEMVANSLN 188

The following BLAST results are available for this feature:

BLAST of Spg023020 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038874456.1	9.6e-67	80.59	gluconokinase [Benincasa hispida] >XP_038874457.1 gluconokinase [Benincasa hispi...	[more]
XP_022995367.1	1.3e-63	80.86	gluconokinase [Cucurbita maxima]	[more]
XP_023532636.1	2.9e-63	80.25	gluconokinase [Cucurbita pepo subsp. pepo]	[more]
XP_004143662.1	3.8e-63	72.53	gluconokinase isoform X1 [Cucumis sativus]	[more]
XP_022159526.1	5.0e-63	77.78	uncharacterized protein LOC111025918 isoform X1 [Momordica charantia]	[more]

BLAST of Spg023020 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SLE0	1.7e-45	56.63	Gluconokinase OS=Arabidopsis thaliana OX=3702 GN=At2g16790 PE=2 SV=2	[more]
Q5T6J7	4.4e-22	38.37	Probable gluconokinase OS=Homo sapiens OX=9606 GN=IDNK PE=1 SV=1	[more]
B0BML1	5.8e-22	39.51	Probable gluconokinase OS=Xenopus tropicalis OX=8364 GN=idnk PE=2 SV=1	[more]
P39208	9.8e-22	43.51	Thermosensitive gluconokinase OS=Escherichia coli (strain K12) OX=83333 GN=idnK ...	[more]
Q5FQ97	1.4e-20	37.97	Gluconokinase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=GOX1709 PE=1 S...	[more]

BLAST of Spg023020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JYN8	6.3e-64	80.86	Gluconokinase OS=Cucurbita maxima OX=3661 GN=LOC111490938 PE=3 SV=1	[more]
A0A0A0KN86	1.8e-63	72.53	Gluconokinase OS=Cucumis sativus OX=3659 GN=Csa_5G169050 PE=3 SV=1	[more]
A0A6J1E468	2.4e-63	77.78	Gluconokinase OS=Momordica charantia OX=3673 GN=LOC111025918 PE=3 SV=1	[more]
A0A6J1H380	5.3e-63	79.63	Gluconokinase OS=Cucurbita moschata OX=3662 GN=LOC111459704 PE=3 SV=1	[more]
A0A1S3CTF0	1.2e-62	72.53	Gluconokinase OS=Cucumis melo OX=3656 GN=LOC103504681 PE=3 SV=1	[more]

BLAST of Spg023020 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G16790.2	3.1e-47	52.41	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT2G16790.1	1.2e-46	56.63	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR031322	Shikimate kinase/gluconokinase	PFAM	PF01202	SKI	coord: 50..177 e-value: 7.2E-6 score: 26.2
IPR006001	Carbohydrate kinase, thermoresistant glucokinase	TIGRFAM	TIGR01313	TIGR01313	coord: 49..205 e-value: 1.5E-36 score: 123.6
IPR006001	Carbohydrate kinase, thermoresistant glucokinase	PANTHER	PTHR43442	GLUCONOKINASE-RELATED	coord: 29..210
IPR006001	Carbohydrate kinase, thermoresistant glucokinase	CDD	cd02021	GntK	coord: 50..204 e-value: 1.01335E-43 score: 142.006
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 48..209 e-value: 2.6E-45 score: 156.2
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 50..210

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg023020.1	Spg023020.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046177	D-gluconate catabolic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0046316	gluconokinase activity
molecular_function	GO:0016301	kinase activity