Spg022732 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022732
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	chaperone protein dnaJ 49-like
Location	scaffold2: 6607370 .. 6608455 (-)
RNA-Seq Expression	Spg022732
Synteny	Spg022732

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGGGAATAAGGATGATGCTTTGAAATGTTTGAGAATTGGAAAAGAGGCTTTGGAAGCTGGGAATTTGGTCCGAGCCCTCAAATTTGTCAATAAAGCTCGTCGTCTTGATCCGACGCTTCCCGTCGACGACCTGTTACCGGAACTTAAGAACGTTTCTGGCGATCAGTTGGGTGGTGATGCGTGTGAATCTGGTAATGGGTCATCTGGTTCCCCTTCATCCAAGTCTTCTGAACAGCCTTCTCTTCGCCGTAGAGTTCCGGCATCTGTTTCTTCTGAATCTTCAACGTCGGCTACTTATACCGAGGAGCACGTTGAGATTGTAAGGCAGATCAAGAAGAAGAAGGATTACTACGAGATTTTGGGATTGGATAAGAGTTGCACTGTTGAGGATGTTCGGAAGTCTTATAGAAAGCTGTCTTTAAAGGTCCATCCAGATAAGAACAATGCTCCTGGTGCCGAAGAAGCGTTTAAGGCTGTATCAAAGGCATTTCAGTGTCTAAGTAATGAGGAGAGTAGAAAAAGGTACGATCTTGTTGGGTCCGAGGAACAAGTCTATGAGAGACGGACTGCAAGACATGGTGCAAATGGATTTAATGGGTTTTATGAAGGTGATGTTGATGCAGAGGAGATATTTAGAAACTTCTTCTTTGGTGGAATGCCACCGACAACTACACATTTCCGTAGTTTCAATTTTGGCCCTGGAATGGCACCTAGAACTGCTGATCATGGCTCTGGTGGTGGCCTTCGGACACTGATACAATTGGTTCCTGTGCTGGTAATATTGCTTTTGAACTTTATGCCATCCTCAGAACCCATCTTTGCACTTTCCCGTTCATATCCTTATGAACACCGATTTACAACTCAGAAGGGTGTCAATTATTATGTGAAATCGAAAAACTTTGAACAAGATTTTCCACCAAATAGTCAGGATCGTCTGGTACTTGAGCAACGGGCAGATAGGGAATACTTCAATATTCTTGCACAGAATTGTCGACTTGAGGTGCAACGACGCCAGTGGGGATTCATTCGTGAAACTCCACATTGTGATATGTTGAATAAGTTTGAATCAGCGGCCCAATGA

mRNA sequence

ATGGATGGGAATAAGGATGATGCTTTGAAATGTTTGAGAATTGGAAAAGAGGCTTTGGAAGCTGGGAATTTGGTCCGAGCCCTCAAATTTGTCAATAAAGCTCGTCGTCTTGATCCGACGCTTCCCGTCGACGACCTGTTACCGGAACTTAAGAACGTTTCTGGCGATCAGTTGGGTGGTGATGCGTGTGAATCTGGTAATGGGTCATCTGGTTCCCCTTCATCCAAGTCTTCTGAACAGCCTTCTCTTCGCCGTAGAGTTCCGGCATCTGTTTCTTCTGAATCTTCAACGTCGGCTACTTATACCGAGGAGCACGTTGAGATTGTAAGGCAGATCAAGAAGAAGAAGGATTACTACGAGATTTTGGGATTGGATAAGAGTTGCACTGTTGAGGATGTTCGGAAGTCTTATAGAAAGCTGTCTTTAAAGGTCCATCCAGATAAGAACAATGCTCCTGGTGCCGAAGAAGCGTTTAAGGCTGTATCAAAGGCATTTCAGTGTCTAAGTAATGAGGAGAGTAGAAAAAGGTACGATCTTGTTGGGTCCGAGGAACAAGTCTATGAGAGACGGACTGCAAGACATGGTGCAAATGGATTTAATGGGTTTTATGAAGGTGATGTTGATGCAGAGGAGATATTTAGAAACTTCTTCTTTGGTGGAATGCCACCGACAACTACACATTTCCGTAGTTTCAATTTTGGCCCTGGAATGGCACCTAGAACTGCTGATCATGGCTCTGGTGGTGGCCTTCGGACACTGATACAATTGGTTCCTGTGCTGGTAATATTGCTTTTGAACTTTATGCCATCCTCAGAACCCATCTTTGCACTTTCCCGTTCATATCCTTATGAACACCGATTTACAACTCAGAAGGGTGTCAATTATTATGTGAAATCGAAAAACTTTGAACAAGATTTTCCACCAAATAGTCAGGATCGTCTGGTACTTGAGCAACGGGCAGATAGGGAATACTTCAATATTCTTGCACAGAATTGTCGACTTGAGGTGCAACGACGCCAGTGGGGATTCATTCGTGAAACTCCACATTGTGATATGTTGAATAAGTTTGAATCAGCGGCCCAATGA

Coding sequence (CDS)

ATGGATGGGAATAAGGATGATGCTTTGAAATGTTTGAGAATTGGAAAAGAGGCTTTGGAAGCTGGGAATTTGGTCCGAGCCCTCAAATTTGTCAATAAAGCTCGTCGTCTTGATCCGACGCTTCCCGTCGACGACCTGTTACCGGAACTTAAGAACGTTTCTGGCGATCAGTTGGGTGGTGATGCGTGTGAATCTGGTAATGGGTCATCTGGTTCCCCTTCATCCAAGTCTTCTGAACAGCCTTCTCTTCGCCGTAGAGTTCCGGCATCTGTTTCTTCTGAATCTTCAACGTCGGCTACTTATACCGAGGAGCACGTTGAGATTGTAAGGCAGATCAAGAAGAAGAAGGATTACTACGAGATTTTGGGATTGGATAAGAGTTGCACTGTTGAGGATGTTCGGAAGTCTTATAGAAAGCTGTCTTTAAAGGTCCATCCAGATAAGAACAATGCTCCTGGTGCCGAAGAAGCGTTTAAGGCTGTATCAAAGGCATTTCAGTGTCTAAGTAATGAGGAGAGTAGAAAAAGGTACGATCTTGTTGGGTCCGAGGAACAAGTCTATGAGAGACGGACTGCAAGACATGGTGCAAATGGATTTAATGGGTTTTATGAAGGTGATGTTGATGCAGAGGAGATATTTAGAAACTTCTTCTTTGGTGGAATGCCACCGACAACTACACATTTCCGTAGTTTCAATTTTGGCCCTGGAATGGCACCTAGAACTGCTGATCATGGCTCTGGTGGTGGCCTTCGGACACTGATACAATTGGTTCCTGTGCTGGTAATATTGCTTTTGAACTTTATGCCATCCTCAGAACCCATCTTTGCACTTTCCCGTTCATATCCTTATGAACACCGATTTACAACTCAGAAGGGTGTCAATTATTATGTGAAATCGAAAAACTTTGAACAAGATTTTCCACCAAATAGTCAGGATCGTCTGGTACTTGAGCAACGGGCAGATAGGGAATACTTCAATATTCTTGCACAGAATTGTCGACTTGAGGTGCAACGACGCCAGTGGGGATTCATTCGTGAAACTCCACATTGTGATATGTTGAATAAGTTTGAATCAGCGGCCCAATGA

Protein sequence

MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGGDACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYEILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLVGSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGMAPRTADHGSGGGLRTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRFTTQKGVNYYVKSKNFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFESAAQ

Homology

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BLAST of Spg022732 vs. NCBI nr
Match: XP_022144873.1 (chaperone protein dnaJ 49-like [Momordica charantia] >XP_022144874.1 chaperone protein dnaJ 49-like [Momordica charantia] >XP_022144875.1 chaperone protein dnaJ 49-like [Momordica charantia])

HSP 1 Score: 670.6 bits (1729), Expect = 7.5e-189
Identity = 334/360 (92.78%), Postives = 348/360 (96.67%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           MDGNKDDALKCL+IGKEALEAGNLVRALKFVNKARRLDP LP+DDLLPELKNVSG+QLG 
Sbjct: 1   MDGNKDDALKCLKIGKEALEAGNLVRALKFVNKARRLDPALPIDDLLPELKNVSGEQLGA 60

Query: 61  DACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
           DACE+GNGSSGSPSSKSS+QPSLRRRVPAS SSESSTSATYTEEHVEIVRQIKKKKDYYE
Sbjct: 61  DACEAGNGSSGSPSSKSSDQPSLRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120

Query: 121 ILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGL+KSC++EDVRK+YRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYD+V
Sbjct: 121 ILGLEKSCSIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDIV 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGMAPR 240
           GSEE VYERRTARHGANGFNGFYE DVDAEEIFRNFFFGGM PTTTHFRSFNFGPGM  R
Sbjct: 181 GSEEPVYERRTARHGANGFNGFYEADVDAEEIFRNFFFGGMAPTTTHFRSFNFGPGMGHR 240

Query: 241 TADHGSGGGLRTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRFTTQKGVNYYVKSK 300
           T DHGSGG  RTLIQL+PVLVILLLNFMPSSEPI+ALSRSYPYEHRFTTQKGVNYYVKSK
Sbjct: 241 TGDHGSGGVFRTLIQLLPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKSK 300

Query: 301 NFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFESAA 360
           NFEQDFPPNS+DRLVLEQR DREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKF+SAA
Sbjct: 301 NFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQSAA 360

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BLAST of Spg022732 vs. NCBI nr
Match: XP_022987765.1 (chaperone protein dnaJ 49-like [Cucurbita maxima])

HSP 1 Score: 670.2 bits (1728), Expect = 9.7e-189
Identity = 335/361 (92.80%), Postives = 352/361 (97.51%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG
Sbjct: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60

Query: 61  DACESGNGSSGSPSSKSSE-QPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYY 120
           DAC++G+G SGSPSSKS E QPS+RRRVPAS SSESSTSATYTEE+VEIVRQIKKKKDYY
Sbjct: 61  DACDAGHGPSGSPSSKSPEQQPSVRRRVPASGSSESSTSATYTEENVEIVRQIKKKKDYY 120

Query: 121 EILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDL 180
           EILGL+KSC++EDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRK YDL
Sbjct: 121 EILGLEKSCSIEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKTYDL 180

Query: 181 VGSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGMAP 240
           VGSEE+VYERRTARHG NGFNGFYEGDVDA+EIFRNFFFGGMPPTTTHFRSFNFGPGMAP
Sbjct: 181 VGSEEEVYERRTARHGGNGFNGFYEGDVDADEIFRNFFFGGMPPTTTHFRSFNFGPGMAP 240

Query: 241 RTADHGSGGGLRTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRFTTQKGVNYYVKS 300
           RT D GSGGGLRTLIQL+PVLVILLLNFMPSSEPI+ALSRSYPYEHRFTTQKGVNYYVKS
Sbjct: 241 RTGDQGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKS 300

Query: 301 KNFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFESA 360
           KNFEQ+FPPNS+DRLVL+QR DREYFNILAQNCRLE+QRRQWGFIRETPHCDMLNKF+SA
Sbjct: 301 KNFEQEFPPNSRDRLVLDQRVDREYFNILAQNCRLEMQRRQWGFIRETPHCDMLNKFQSA 360

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BLAST of Spg022732 vs. NCBI nr
Match: XP_008444016.1 (PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo] >XP_016899821.1 PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo] >TYK26921.1 chaperone protein dnaJ 49-like [Cucumis melo var. makuwa])

HSP 1 Score: 669.8 bits (1727), Expect = 1.3e-188
Identity = 332/360 (92.22%), Postives = 350/360 (97.22%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           MDGNKDD LKCLRIGKEALEAGNL RALKFVNKARRLDPTL VDDLLP+LKNVSGDQLGG
Sbjct: 1   MDGNKDDGLKCLRIGKEALEAGNLTRALKFVNKARRLDPTLHVDDLLPKLKNVSGDQLGG 60

Query: 61  DACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
           DACE+GNGSSGS SSKS EQPS+RRRVPAS SSESSTS TY EEHVEIVRQ+KKKKDYYE
Sbjct: 61  DACEAGNGSSGSSSSKSPEQPSVRRRVPASGSSESSTSTTYNEEHVEIVRQLKKKKDYYE 120

Query: 121 ILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGL+KSCT+EDVRK+YRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYD+V
Sbjct: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDVV 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGMAPR 240
           GSEEQVYERRTARHGA+GFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPG+APR
Sbjct: 181 GSEEQVYERRTARHGAHGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGVAPR 240

Query: 241 TADHGSGGGLRTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRFTTQKGVNYYVKSK 300
           T DHGSGGGLRTLIQL+PVLVILLLNFMPSSEPI+ALSRSYPYEHRFTTQKGVNYYVKSK
Sbjct: 241 TGDHGSGGGLRTLIQLIPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKSK 300

Query: 301 NFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFESAA 360
           NFEQDFPP+S+DRLVLEQ+ DREYF+ILAQNCRLEVQRRQWGFIRETPHCDML+KF+SAA
Sbjct: 301 NFEQDFPPSSRDRLVLEQKVDREYFDILAQNCRLEVQRRQWGFIRETPHCDMLDKFQSAA 360

[Close]

BLAST of Spg022732 vs. NCBI nr
Match: KAA0046789.1 (chaperone protein dnaJ 49-like [Cucumis melo var. makuwa])

HSP 1 Score: 669.8 bits (1727), Expect = 1.3e-188
Identity = 332/360 (92.22%), Postives = 350/360 (97.22%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           MDGNKDD LKCLRIGKEALEAGNL RALKFVNKARRLDPTL VDDLLP+LKNVSGDQLGG
Sbjct: 125 MDGNKDDGLKCLRIGKEALEAGNLTRALKFVNKARRLDPTLHVDDLLPKLKNVSGDQLGG 184

Query: 61  DACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
           DACE+GNGSSGS SSKS EQPS+RRRVPAS SSESSTS TY EEHVEIVRQ+KKKKDYYE
Sbjct: 185 DACEAGNGSSGSSSSKSPEQPSVRRRVPASGSSESSTSTTYNEEHVEIVRQLKKKKDYYE 244

Query: 121 ILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGL+KSCT+EDVRK+YRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYD+V
Sbjct: 245 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDVV 304

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGMAPR 240
           GSEEQVYERRTARHGA+GFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPG+APR
Sbjct: 305 GSEEQVYERRTARHGAHGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGVAPR 364

Query: 241 TADHGSGGGLRTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRFTTQKGVNYYVKSK 300
           T DHGSGGGLRTLIQL+PVLVILLLNFMPSSEPI+ALSRSYPYEHRFTTQKGVNYYVKSK
Sbjct: 365 TGDHGSGGGLRTLIQLIPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKSK 424

Query: 301 NFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFESAA 360
           NFEQDFPP+S+DRLVLEQ+ DREYF+ILAQNCRLEVQRRQWGFIRETPHCDML+KF+SAA
Sbjct: 425 NFEQDFPPSSRDRLVLEQKVDREYFDILAQNCRLEVQRRQWGFIRETPHCDMLDKFQSAA 484

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BLAST of Spg022732 vs. NCBI nr
Match: XP_022961054.1 (chaperone protein dnaJ 49-like [Cucurbita moschata])

HSP 1 Score: 666.8 bits (1719), Expect = 1.1e-187
Identity = 334/361 (92.52%), Postives = 351/361 (97.23%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG
Sbjct: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60

Query: 61  DACESGNGSSGSPSSKSSE-QPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYY 120
           DAC++GNG SGSPSSKS E QPS+RRRVPA  SSESSTSATYTEE+VEIVRQIKKKKDYY
Sbjct: 61  DACDAGNGPSGSPSSKSPEQQPSVRRRVPA--SSESSTSATYTEENVEIVRQIKKKKDYY 120

Query: 121 EILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDL 180
           EILGL+KSC++EDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRK YDL
Sbjct: 121 EILGLEKSCSIEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKTYDL 180

Query: 181 VGSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGMAP 240
           VGSEE++YERRTARHG NGFNGFYEGDVDA+EIFRNFFFGGMPPTTTHFRSFNFGPGMAP
Sbjct: 181 VGSEEEIYERRTARHGGNGFNGFYEGDVDADEIFRNFFFGGMPPTTTHFRSFNFGPGMAP 240

Query: 241 RTADHGSGGGLRTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRFTTQKGVNYYVKS 300
           RT D GSGGGLRTLIQL+PVLVILLLNFMPSSEPI+ALSRSYPYEHRFTTQKGVNYYVKS
Sbjct: 241 RTGDQGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKS 300

Query: 301 KNFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFESA 360
           KNFEQ+FPPNS+DRLVL+QR DREYFNILAQNCRLE+QRRQWGFIRETPHCDMLNKF+SA
Sbjct: 301 KNFEQEFPPNSRDRLVLDQRVDREYFNILAQNCRLEMQRRQWGFIRETPHCDMLNKFQSA 359

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BLAST of Spg022732 vs. ExPASy Swiss-Prot
Match: Q9FH28 (Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2)

HSP 1 Score: 231.9 bits (590), Expect = 1.2e-59
Identity = 147/361 (40.72%), Postives = 217/361 (60.11%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           MDGNKDDA +CLRI ++A+ +G+  RALKF+N A+RL+P+L VD+L+             
Sbjct: 1   MDGNKDDASRCLRIAEDAIVSGDKERALKFINMAKRLNPSLSVDELVA------------ 60

Query: 61  DACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
            AC+  N  S S +S  SE+    + +        +    YTEE+V++VR I +  DYY 
Sbjct: 61  -ACD--NLDSVSRNSSVSEK---LKTMDGDDDKLETGKMKYTEENVDLVRNIIRNNDYYA 120

Query: 121 ILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGL+K+C+V+++RK+YRKLSLKVHPDKN APG+EEAFK VSKAF CLS+  SR+++D V
Sbjct: 121 ILGLEKNCSVDEIRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFDQV 180

Query: 181 GSEEQV----YERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPG 240
           G  ++        R  R   N  N F++ + D EEIFR  F          FR+ +    
Sbjct: 181 GIVDEFDHVQRRNRRPRRRYNTRNDFFDDEFDPEEIFRTVF----GQQREVFRASHAYRT 240

Query: 241 MAPRT-----ADHGSGGGLRTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRFTTQK 300
             PR        + +G    T+IQ++P  ++LLL ++P SEP ++L ++  Y+   TTQ 
Sbjct: 241 RQPRNQFREEEINVAGPSCLTIIQILPFFLLLLLAYLPFSEPDYSLHKNQSYQIPKTTQN 300

Query: 301 -GVNYYVKSKN-FEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQWGFIRETPH 351
             +++YV+S + F++ FP +S  R  LE    +EY + L Q+CR+E+Q+R+W     TPH
Sbjct: 301 TEISFYVRSASAFDEKFPLSSSARANLEGNVIKEYKHFLFQSCRIELQKRRWNKKIPTPH 339

[Close]

BLAST of Spg022732 vs. ExPASy Swiss-Prot
Match: Q7ZXQ8 (DnaJ homolog subfamily B member 14 OS=Xenopus laevis OX=8355 GN=dnajb14 PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.5e-46
Identity = 130/353 (36.83%), Postives = 191/353 (54.11%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           M+ N+D+A +C+RIGK A+EAG+  +A +F +KA RL P+     LL  L+         
Sbjct: 1   MESNRDEAERCVRIGKAAIEAGDKEKARRFFSKAERLYPSSEARVLLDALEKND------ 60

Query: 61  DACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
               +GNG      SKS+EQP       A   S   T   +T++ V+ V++IKK K YYE
Sbjct: 61  ---TAGNGPQSEKMSKSTEQPK------AEKDSSGDTGKGHTQDQVDGVQRIKKCKTYYE 120

Query: 121 ILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           +LG+      ED++K+YRKL+LK HPDKN+APGA EAFK +  A+  LSN E RK+YDL 
Sbjct: 121 VLGVSPDAGEEDLKKAYRKLALKFHPDKNHAPGATEAFKKIGNAYAVLSNPEKRKQYDLT 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGMAPR 240
           GSE+ V  +   R+G   ++  +E D+  E++F  FF GG P  + H  S N     +  
Sbjct: 181 GSEDNV--QNNHRNGGFDYHRGFEADITPEDLFNMFFGGGFPSGSVHTFS-NGRTRYSHH 240

Query: 241 TADHGSG---------GGLRTLIQLVPVLVILLLN----FMPSSEPIFALSRSYPYEHRF 300
              H SG         GG    IQL+P++V++L++     M S+ P     RS     R 
Sbjct: 241 QHHHHSGHDREEERADGGFSMFIQLMPIIVLILVSLLSQLMVSNPPYSLYPRSGQTIKRV 300

Query: 301 TTQKGVNYYVKSKNFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQ 341
           T    ++YYV SK+F+ ++  N      LE+  + +Y   +  NC  E Q++Q
Sbjct: 301 TENLQISYYV-SKDFKSEY--NGMLLQKLEKNIEEDYVANVRNNCWRERQQKQ 332

[Close]

BLAST of Spg022732 vs. ExPASy Swiss-Prot
Match: Q28I38 (DnaJ homolog subfamily B member 14 OS=Xenopus tropicalis OX=8364 GN=dnajb14 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 3.3e-46
Identity = 126/355 (35.49%), Postives = 194/355 (54.65%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           M+ N+D+A +C+RI K A+EAG+  +A +F++KA RL P+     LL   +         
Sbjct: 1   MESNRDEAERCVRIAKAAIEAGDKEKAKRFLSKAERLYPSSEARALLQAFEKND------ 60

Query: 61  DACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
               +GNG   +  +K +EQP   +   AS SS+  T   +T++ ++ V++IKK K YYE
Sbjct: 61  ---TAGNGPQSAKMAKGTEQPKAEKDSNASASSD--TGKGHTQDQLDGVQRIKKCKTYYE 120

Query: 121 ILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           +LG+      ED++K+YRKL+LK HPDKN+APGA EAFK +  A+  LSN E RK+YDL 
Sbjct: 121 VLGVSTDAGEEDLKKAYRKLALKFHPDKNHAPGATEAFKKIGNAYAVLSNPEKRKQYDLT 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGMAPR 240
           GSE+Q+  +   R+G   ++  +E D+  E++F  FF GG P  + H     F  G A  
Sbjct: 181 GSEDQM--QNNHRNGGFDYHRGFEADITPEDLFNMFFGGGFPSGSVH----TFSNGRARY 240

Query: 241 T------------ADHGSGGGLRTLIQLVPVLVILLLN----FMPSSEPIFALSRSYPYE 300
           +             D  + GG    IQL+P++V++L++    FM S+ P     RS    
Sbjct: 241 SHHQHHHHSGHDREDERADGGFSMFIQLMPIIVLILVSLLSQFMVSNPPYSLYPRSGQAT 300

Query: 301 HRFTTQKGVNYYVKSKNFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRR 340
            R T    + YYV SK+F+ ++      +  LE+  + +Y   +  NC  E Q+R
Sbjct: 301 KRVTENLQIAYYV-SKDFQSEYSGILLQK--LEKNIEEDYVANVRNNCWRERQQR 335

[Close]

BLAST of Spg022732 vs. ExPASy Swiss-Prot
Match: Q9NXW2 (DnaJ homolog subfamily B member 12 OS=Homo sapiens OX=9606 GN=DNAJB12 PE=1 SV=5)

HSP 1 Score: 176.8 bits (447), Expect = 4.4e-43
Identity = 123/365 (33.70%), Postives = 186/365 (50.96%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELK---NVSGDQ 60
           M+ NKD+A +C+ I  +A+++    RAL+F+ KA+RL PT  V  L+  L      +GDQ
Sbjct: 1   MESNKDEAERCISIALKAIQSNQPDRALRFLEKAQRLYPTPRVRALIESLNQKPQTAGDQ 60

Query: 61  -------------LGGDACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEE 120
                         GG    S NG +G  S+K                        YT E
Sbjct: 61  PPPTDTTHATHRKAGGTDAPSANGEAGGESTKG-----------------------YTAE 120

Query: 121 HVEIVRQIKKKKDYYEILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKA 180
            V  V+++K+ KDYYEILG+ +  + ED++K+YR+L+LK HPDKN+APGA EAFKA+  A
Sbjct: 121 QVAAVKRVKQCKDYYEILGVSRGASDEDLKKAYRRLALKFHPDKNHAPGATEAFKAIGTA 180

Query: 181 FQCLSNEESRKRYDLVGSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPT 240
           +  LSN E RK+YD  G ++    R    HG   F+  +E D+  E++F  FF GG P +
Sbjct: 181 YAVLSNPEKRKQYDQFGDDKSQAARH--GHGHGDFHRGFEADISPEDLFNMFFGGGFPSS 240

Query: 241 TTHFRS---FNFGPGMAPRTADHGSGGGLRTLIQLVPVLVILLLNFMPS---SEPIFALS 300
             H  S     +         D+   GGL   +QL+P+L+++L++ +     S P ++LS
Sbjct: 241 NVHVYSNGRMRYTYQQRQDRRDNQGDGGLGVFVQLMPILILILVSALSQLMVSSPPYSLS 300

Query: 301 RSYPYEH---RFTTQKGVNYYVKSKNFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLE 341
                 H   R T   GV YYV    F +++  +S     +E+  + +Y   L  NC  E
Sbjct: 301 PRPSVGHIHRRVTDHLGVVYYV-GDTFSEEYTGSSLK--TVERNVEDDYIANLRNNCWKE 337

[Close]

BLAST of Spg022732 vs. ExPASy Swiss-Prot
Match: Q9QYI4 (DnaJ homolog subfamily B member 12 OS=Mus musculus OX=10090 GN=Dnajb12 PE=1 SV=2)

HSP 1 Score: 175.3 bits (443), Expect = 1.3e-42
Identity = 119/349 (34.10%), Postives = 186/349 (53.30%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           M+ NKD+A +C+ I  +A+++    RAL+F+ KA+RL PT  V  L+  L N      G 
Sbjct: 1   MESNKDEAERCISIALKAIQSNQPERALRFLEKAQRLYPTPRVSALIESL-NQKPQSTGD 60

Query: 61  DACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
               +    + +  +  +E PS         S++      YT E V  V+++K+ KDYYE
Sbjct: 61  HPQPTDTTHTTTKKAGGTETPSANGEAGGGESAKG-----YTSEQVAAVKRVKQCKDYYE 120

Query: 121 ILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILG+ +S + ED++K+YRKL+LK HPDKN+APGA EAFKA+  A+  LSN E RK+YD  
Sbjct: 121 ILGVSRSASDEDLKKAYRKLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYDQF 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRS---FNFGPGM 240
           G ++    R    HG   F+  +E D+  E++F  FF GG P +  H  S     +    
Sbjct: 181 GDDKSQAARHGHSHG--DFHRGFEADISPEDLFNMFFGGGFPSSNVHVYSNGRMRYTYQQ 240

Query: 241 APRTADHGSGGGLRTLIQLVPVLVILLLNFMPS---SEPIFALSRSYPYEH---RFTTQK 300
                D+   GGL   +QL+P+L+++L++ +     S P ++LS      H   R T   
Sbjct: 241 RQDRRDNQGDGGLGVFVQLMPILILILVSALSQLMVSSPPYSLSPRPSVGHIHKRVTDHL 300

Query: 301 GVNYYVKSKNFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQ 341
            V YYV +  F +++  +S     +E+  + +Y   L  NC  E Q+++
Sbjct: 301 NVAYYV-ADTFSEEYTGSSLK--TVERNVEDDYIANLRNNCWKEKQQKE 338

[Close]

BLAST of Spg022732 vs. ExPASy TrEMBL
Match: A0A6J1CTJ0 (chaperone protein dnaJ 49-like OS=Momordica charantia OX=3673 GN=LOC111014450 PE=4 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 3.6e-189
Identity = 334/360 (92.78%), Postives = 348/360 (96.67%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           MDGNKDDALKCL+IGKEALEAGNLVRALKFVNKARRLDP LP+DDLLPELKNVSG+QLG 
Sbjct: 1   MDGNKDDALKCLKIGKEALEAGNLVRALKFVNKARRLDPALPIDDLLPELKNVSGEQLGA 60

Query: 61  DACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
           DACE+GNGSSGSPSSKSS+QPSLRRRVPAS SSESSTSATYTEEHVEIVRQIKKKKDYYE
Sbjct: 61  DACEAGNGSSGSPSSKSSDQPSLRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120

Query: 121 ILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGL+KSC++EDVRK+YRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYD+V
Sbjct: 121 ILGLEKSCSIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDIV 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGMAPR 240
           GSEE VYERRTARHGANGFNGFYE DVDAEEIFRNFFFGGM PTTTHFRSFNFGPGM  R
Sbjct: 181 GSEEPVYERRTARHGANGFNGFYEADVDAEEIFRNFFFGGMAPTTTHFRSFNFGPGMGHR 240

Query: 241 TADHGSGGGLRTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRFTTQKGVNYYVKSK 300
           T DHGSGG  RTLIQL+PVLVILLLNFMPSSEPI+ALSRSYPYEHRFTTQKGVNYYVKSK
Sbjct: 241 TGDHGSGGVFRTLIQLLPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKSK 300

Query: 301 NFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFESAA 360
           NFEQDFPPNS+DRLVLEQR DREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKF+SAA
Sbjct: 301 NFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQSAA 360

[Close]

BLAST of Spg022732 vs. ExPASy TrEMBL
Match: A0A6J1JHS2 (chaperone protein dnaJ 49-like OS=Cucurbita maxima OX=3661 GN=LOC111485216 PE=4 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 4.7e-189
Identity = 335/361 (92.80%), Postives = 352/361 (97.51%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG
Sbjct: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60

Query: 61  DACESGNGSSGSPSSKSSE-QPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYY 120
           DAC++G+G SGSPSSKS E QPS+RRRVPAS SSESSTSATYTEE+VEIVRQIKKKKDYY
Sbjct: 61  DACDAGHGPSGSPSSKSPEQQPSVRRRVPASGSSESSTSATYTEENVEIVRQIKKKKDYY 120

Query: 121 EILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDL 180
           EILGL+KSC++EDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRK YDL
Sbjct: 121 EILGLEKSCSIEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKTYDL 180

Query: 181 VGSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGMAP 240
           VGSEE+VYERRTARHG NGFNGFYEGDVDA+EIFRNFFFGGMPPTTTHFRSFNFGPGMAP
Sbjct: 181 VGSEEEVYERRTARHGGNGFNGFYEGDVDADEIFRNFFFGGMPPTTTHFRSFNFGPGMAP 240

Query: 241 RTADHGSGGGLRTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRFTTQKGVNYYVKS 300
           RT D GSGGGLRTLIQL+PVLVILLLNFMPSSEPI+ALSRSYPYEHRFTTQKGVNYYVKS
Sbjct: 241 RTGDQGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKS 300

Query: 301 KNFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFESA 360
           KNFEQ+FPPNS+DRLVL+QR DREYFNILAQNCRLE+QRRQWGFIRETPHCDMLNKF+SA
Sbjct: 301 KNFEQEFPPNSRDRLVLDQRVDREYFNILAQNCRLEMQRRQWGFIRETPHCDMLNKFQSA 360

[Close]

BLAST of Spg022732 vs. ExPASy TrEMBL
Match: A0A1S3BA76 (chaperone protein dnaJ 49-like OS=Cucumis melo OX=3656 GN=LOC103487463 PE=4 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 6.2e-189
Identity = 332/360 (92.22%), Postives = 350/360 (97.22%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           MDGNKDD LKCLRIGKEALEAGNL RALKFVNKARRLDPTL VDDLLP+LKNVSGDQLGG
Sbjct: 1   MDGNKDDGLKCLRIGKEALEAGNLTRALKFVNKARRLDPTLHVDDLLPKLKNVSGDQLGG 60

Query: 61  DACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
           DACE+GNGSSGS SSKS EQPS+RRRVPAS SSESSTS TY EEHVEIVRQ+KKKKDYYE
Sbjct: 61  DACEAGNGSSGSSSSKSPEQPSVRRRVPASGSSESSTSTTYNEEHVEIVRQLKKKKDYYE 120

Query: 121 ILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGL+KSCT+EDVRK+YRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYD+V
Sbjct: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDVV 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGMAPR 240
           GSEEQVYERRTARHGA+GFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPG+APR
Sbjct: 181 GSEEQVYERRTARHGAHGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGVAPR 240

Query: 241 TADHGSGGGLRTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRFTTQKGVNYYVKSK 300
           T DHGSGGGLRTLIQL+PVLVILLLNFMPSSEPI+ALSRSYPYEHRFTTQKGVNYYVKSK
Sbjct: 241 TGDHGSGGGLRTLIQLIPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKSK 300

Query: 301 NFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFESAA 360
           NFEQDFPP+S+DRLVLEQ+ DREYF+ILAQNCRLEVQRRQWGFIRETPHCDML+KF+SAA
Sbjct: 301 NFEQDFPPSSRDRLVLEQKVDREYFDILAQNCRLEVQRRQWGFIRETPHCDMLDKFQSAA 360

[Close]

BLAST of Spg022732 vs. ExPASy TrEMBL
Match: A0A5A7TZX0 (Chaperone protein dnaJ 49-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216G00790 PE=4 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 6.2e-189
Identity = 332/360 (92.22%), Postives = 350/360 (97.22%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           MDGNKDD LKCLRIGKEALEAGNL RALKFVNKARRLDPTL VDDLLP+LKNVSGDQLGG
Sbjct: 125 MDGNKDDGLKCLRIGKEALEAGNLTRALKFVNKARRLDPTLHVDDLLPKLKNVSGDQLGG 184

Query: 61  DACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
           DACE+GNGSSGS SSKS EQPS+RRRVPAS SSESSTS TY EEHVEIVRQ+KKKKDYYE
Sbjct: 185 DACEAGNGSSGSSSSKSPEQPSVRRRVPASGSSESSTSTTYNEEHVEIVRQLKKKKDYYE 244

Query: 121 ILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGL+KSCT+EDVRK+YRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYD+V
Sbjct: 245 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDVV 304

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGMAPR 240
           GSEEQVYERRTARHGA+GFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPG+APR
Sbjct: 305 GSEEQVYERRTARHGAHGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGVAPR 364

Query: 241 TADHGSGGGLRTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRFTTQKGVNYYVKSK 300
           T DHGSGGGLRTLIQL+PVLVILLLNFMPSSEPI+ALSRSYPYEHRFTTQKGVNYYVKSK
Sbjct: 365 TGDHGSGGGLRTLIQLIPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKSK 424

Query: 301 NFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFESAA 360
           NFEQDFPP+S+DRLVLEQ+ DREYF+ILAQNCRLEVQRRQWGFIRETPHCDML+KF+SAA
Sbjct: 425 NFEQDFPPSSRDRLVLEQKVDREYFDILAQNCRLEVQRRQWGFIRETPHCDMLDKFQSAA 484

[Close]

BLAST of Spg022732 vs. ExPASy TrEMBL
Match: A0A5D3DT80 (Chaperone protein dnaJ 49-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold876G00010 PE=4 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 6.2e-189
Identity = 332/360 (92.22%), Postives = 350/360 (97.22%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           MDGNKDD LKCLRIGKEALEAGNL RALKFVNKARRLDPTL VDDLLP+LKNVSGDQLGG
Sbjct: 1   MDGNKDDGLKCLRIGKEALEAGNLTRALKFVNKARRLDPTLHVDDLLPKLKNVSGDQLGG 60

Query: 61  DACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
           DACE+GNGSSGS SSKS EQPS+RRRVPAS SSESSTS TY EEHVEIVRQ+KKKKDYYE
Sbjct: 61  DACEAGNGSSGSSSSKSPEQPSVRRRVPASGSSESSTSTTYNEEHVEIVRQLKKKKDYYE 120

Query: 121 ILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGL+KSCT+EDVRK+YRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYD+V
Sbjct: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDVV 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGMAPR 240
           GSEEQVYERRTARHGA+GFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPG+APR
Sbjct: 181 GSEEQVYERRTARHGAHGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGVAPR 240

Query: 241 TADHGSGGGLRTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRFTTQKGVNYYVKSK 300
           T DHGSGGGLRTLIQL+PVLVILLLNFMPSSEPI+ALSRSYPYEHRFTTQKGVNYYVKSK
Sbjct: 241 TGDHGSGGGLRTLIQLIPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKSK 300

Query: 301 NFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFESAA 360
           NFEQDFPP+S+DRLVLEQ+ DREYF+ILAQNCRLEVQRRQWGFIRETPHCDML+KF+SAA
Sbjct: 301 NFEQDFPPSSRDRLVLEQKVDREYFDILAQNCRLEVQRRQWGFIRETPHCDMLDKFQSAA 360

[Close]

BLAST of Spg022732 vs. TAIR 10
Match: AT3G57340.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 429.5 bits (1103), Expect = 2.7e-120
Identity = 224/373 (60.05%), Postives = 292/373 (78.28%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           MDGNKDDALKCL+I K A+EAG+  RALKF+ KARRLDP LP+DDL+ EL N   D+ G 
Sbjct: 1   MDGNKDDALKCLKICKSAMEAGDRPRALKFLAKARRLDPNLPIDDLVSELNNNKSDEPG- 60

Query: 61  DACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
            + +S   ++   SS SS++PSLR+R   S ++ SS+S +YTEE + IVR+IK KKDYYE
Sbjct: 61  -SAKSPGSAAAKDSSNSSDRPSLRQR--GSSTTSSSSSMSYTEEQISIVRKIKSKKDYYE 120

Query: 121 ILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGL+ +C+V+DVRK+YRKLSLKVHPDKN APG+EEAFK+VSKAFQCLSN+E+RK+YD+ 
Sbjct: 121 ILGLESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSKAFQCLSNDEARKKYDVS 180

Query: 181 GSEEQVYE-RRTARHGANGFNG--FYEGDVDAEEIFRNFF------FGGMPPTTTHFRSF 240
           GS+E +Y+ RR+AR  +NGFNG  +YE + D  EIFR+FF       GGMPP T  FRSF
Sbjct: 181 GSDEPIYQPRRSAR--SNGFNGGYYYEDEFDPNEIFRSFFGGGGFGGGGMPPATAQFRSF 240

Query: 241 NFGPGMAPRTADHG----SGGGLRTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRF 300
           NFG     RTA++     +G   R L+QL+PV+ ILLLNFMPSS+P++ LS +YPY+++F
Sbjct: 241 NFG-ATRQRTANNNQAPDAGFNARILLQLLPVVFILLLNFMPSSQPVYQLSATYPYQYKF 300

Query: 301 TTQKGVNYYVKSKNFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQWGFIRET 360
           TTQKGVNY+VKS  FEQD+P +S DR  LE++ +R+Y +IL+QNCR E+QR+QWGF+RET
Sbjct: 301 TTQKGVNYFVKSSKFEQDYPRDSNDRHTLEEQVERDYVSILSQNCRYEMQRKQWGFVRET 360

[Close]

BLAST of Spg022732 vs. TAIR 10
Match: AT3G57340.2 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 429.5 bits (1103), Expect = 2.7e-120
Identity = 224/373 (60.05%), Postives = 292/373 (78.28%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           MDGNKDDALKCL+I K A+EAG+  RALKF+ KARRLDP LP+DDL+ EL N   D+ G 
Sbjct: 1   MDGNKDDALKCLKICKSAMEAGDRPRALKFLAKARRLDPNLPIDDLVSELNNNKSDEPG- 60

Query: 61  DACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
            + +S   ++   SS SS++PSLR+R   S ++ SS+S +YTEE + IVR+IK KKDYYE
Sbjct: 61  -SAKSPGSAAAKDSSNSSDRPSLRQR--GSSTTSSSSSMSYTEEQISIVRKIKSKKDYYE 120

Query: 121 ILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGL+ +C+V+DVRK+YRKLSLKVHPDKN APG+EEAFK+VSKAFQCLSN+E+RK+YD+ 
Sbjct: 121 ILGLESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSKAFQCLSNDEARKKYDVS 180

Query: 181 GSEEQVYE-RRTARHGANGFNG--FYEGDVDAEEIFRNFF------FGGMPPTTTHFRSF 240
           GS+E +Y+ RR+AR  +NGFNG  +YE + D  EIFR+FF       GGMPP T  FRSF
Sbjct: 181 GSDEPIYQPRRSAR--SNGFNGGYYYEDEFDPNEIFRSFFGGGGFGGGGMPPATAQFRSF 240

Query: 241 NFGPGMAPRTADHG----SGGGLRTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRF 300
           NFG     RTA++     +G   R L+QL+PV+ ILLLNFMPSS+P++ LS +YPY+++F
Sbjct: 241 NFG-ATRQRTANNNQAPDAGFNARILLQLLPVVFILLLNFMPSSQPVYQLSATYPYQYKF 300

Query: 301 TTQKGVNYYVKSKNFEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQWGFIRET 360
           TTQKGVNY+VKS  FEQD+P +S DR  LE++ +R+Y +IL+QNCR E+QR+QWGF+RET
Sbjct: 301 TTQKGVNYFVKSSKFEQDYPRDSNDRHTLEEQVERDYVSILSQNCRYEMQRKQWGFVRET 360

[Close]

BLAST of Spg022732 vs. TAIR 10
Match: AT5G05750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 314.3 bits (804), Expect = 1.3e-85
Identity = 175/292 (59.93%), Postives = 220/292 (75.34%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           MDGNKDDALKCL+IGK+A+EAG+  RALKF+ KARRLDP LP+D L+ +LK  S +    
Sbjct: 1   MDGNKDDALKCLKIGKDAIEAGDRSRALKFLEKARRLDPNLPIDGLVSDLKKQSDEPA-- 60

Query: 61  DACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
            A E   GS+ + SSK S++PSLR+R  +S ++ SS+S++ TEE   IVR+IK KKDYYE
Sbjct: 61  -AEEDSPGSAANESSKPSDRPSLRQRGSSSSAAGSSSSSSSTEEQRTIVREIKSKKDYYE 120

Query: 121 ILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGL  +C+VED+RKSYRKLSLKVHPDKN APG+EEAFK+VSKAFQCLSNE++R++YD  
Sbjct: 121 ILGLKSNCSVEDLRKSYRKLSLKVHPDKNKAPGSEEAFKSVSKAFQCLSNEDTRRKYDGS 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGG-MPPTTTHFRSFNFGPGMAP 240
           GS+E  Y+ R      NGFNGFY+ + DA+EIFR+FF GG M P TT FRSFNFG G   
Sbjct: 181 GSDEPAYQPRRDARRNNGFNGFYDDEFDADEIFRSFFGGGEMNPATTQFRSFNFGGG--T 240

Query: 241 RTADHGSGGGL--RTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRFTT 290
           RTA+  S  G   R L+Q++PV+ ILLLNF+PS +PI++LS      HR TT
Sbjct: 241 RTANQASDTGFNPRVLLQILPVVFILLLNFLPSPQPIYSLS------HRITT 281

[Close]

BLAST of Spg022732 vs. TAIR 10
Match: AT5G49060.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 231.9 bits (590), Expect = 8.3e-61
Identity = 147/361 (40.72%), Postives = 217/361 (60.11%), Query Frame = 0


Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60
           MDGNKDDA +CLRI ++A+ +G+  RALKF+N A+RL+P+L VD+L+             
Sbjct: 1   MDGNKDDASRCLRIAEDAIVSGDKERALKFINMAKRLNPSLSVDELVA------------ 60

Query: 61  DACESGNGSSGSPSSKSSEQPSLRRRVPASVSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
            AC+  N  S S +S  SE+    + +        +    YTEE+V++VR I +  DYY 
Sbjct: 61  -ACD--NLDSVSRNSSVSEK---LKTMDGDDDKLETGKMKYTEENVDLVRNIIRNNDYYA 120

Query: 121 ILGLDKSCTVEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGL+K+C+V+++RK+YRKLSLKVHPDKN APG+EEAFK VSKAF CLS+  SR+++D V
Sbjct: 121 ILGLEKNCSVDEIRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFDQV 180

Query: 181 GSEEQV----YERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPG 240
           G  ++        R  R   N  N F++ + D EEIFR  F          FR+ +    
Sbjct: 181 GIVDEFDHVQRRNRRPRRRYNTRNDFFDDEFDPEEIFRTVF----GQQREVFRASHAYRT 240

Query: 241 MAPRT-----ADHGSGGGLRTLIQLVPVLVILLLNFMPSSEPIFALSRSYPYEHRFTTQK 300
             PR        + +G    T+IQ++P  ++LLL ++P SEP ++L ++  Y+   TTQ 
Sbjct: 241 RQPRNQFREEEINVAGPSCLTIIQILPFFLLLLLAYLPFSEPDYSLHKNQSYQIPKTTQN 300

Query: 301 -GVNYYVKSKN-FEQDFPPNSQDRLVLEQRADREYFNILAQNCRLEVQRRQWGFIRETPH 351
             +++YV+S + F++ FP +S  R  LE    +EY + L Q+CR+E+Q+R+W     TPH
Sbjct: 301 TEISFYVRSASAFDEKFPLSSSARANLEGNVIKEYKHFLFQSCRIELQKRRWNKKIPTPH 339

[Close]

BLAST of Spg022732 vs. TAIR 10
Match: AT2G20560.1 (DNAJ heat shock family protein )

HSP 1 Score: 76.3 bits (186), Expect = 5.8e-14
Identity = 54/149 (36.24%), Postives = 78/149 (52.35%), Query Frame = 0


Query: 117 DYYEILGLDKSCTVEDVRKSYRKLSLKVHPDK--NNAPGAEEAFKAVSKAFQCLSNEESR 176
           DYY++L +D+S + +D++K+YRKL++K HPDK  NN   AE  FK +S+A++ LS+ + +
Sbjct: 4   DYYKVLQVDRSASDDDLKKAYRKLAMKWHPDKNPNNKKDAEAMFKQISEAYEVLSDPQKK 63

Query: 177 KRYDLVGSEEQVYERRTARHGANGFNGFYEGD---------VDAEEIFRNFF-----FGG 236
             YD  G  E+  +       A G   F  GD          +A++IF  FF     FGG
Sbjct: 64  AVYDQYG--EEGLKGNVPPPDAGGATYFSTGDGPTSFRFNPRNADDIFAEFFGFSSPFGG 123

Query: 237 MPPTTTHFRSFNFGPGMAPRTADHGSGGG 250
                T F S  FG  M     + G GGG
Sbjct: 124 -GRGGTRFSSSMFGDNMFASFGEGGGGGG 149

The following BLAST results are available for this feature:

BLAST of Spg022732 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144873.1	7.5e-189	92.78	chaperone protein dnaJ 49-like [Momordica charantia] >XP_022144874.1 chaperone p...	[more]
XP_022987765.1	9.7e-189	92.80	chaperone protein dnaJ 49-like [Cucurbita maxima]	[more]
XP_008444016.1	1.3e-188	92.22	PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo] >XP_016899821.1 PREDICT...	[more]
KAA0046789.1	1.3e-188	92.22	chaperone protein dnaJ 49-like [Cucumis melo var. makuwa]	[more]
XP_022961054.1	1.1e-187	92.52	chaperone protein dnaJ 49-like [Cucurbita moschata]	[more]

BLAST of Spg022732 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FH28	1.2e-59	40.72	Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2	[more]
Q7ZXQ8	2.5e-46	36.83	DnaJ homolog subfamily B member 14 OS=Xenopus laevis OX=8355 GN=dnajb14 PE=2 SV=...	[more]
Q28I38	3.3e-46	35.49	DnaJ homolog subfamily B member 14 OS=Xenopus tropicalis OX=8364 GN=dnajb14 PE=2...	[more]
Q9NXW2	4.4e-43	33.70	DnaJ homolog subfamily B member 12 OS=Homo sapiens OX=9606 GN=DNAJB12 PE=1 SV=5	[more]
Q9QYI4	1.3e-42	34.10	DnaJ homolog subfamily B member 12 OS=Mus musculus OX=10090 GN=Dnajb12 PE=1 SV=2	[more]

BLAST of Spg022732 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CTJ0	3.6e-189	92.78	chaperone protein dnaJ 49-like OS=Momordica charantia OX=3673 GN=LOC111014450 PE...	[more]
A0A6J1JHS2	4.7e-189	92.80	chaperone protein dnaJ 49-like OS=Cucurbita maxima OX=3661 GN=LOC111485216 PE=4 ...	[more]
A0A1S3BA76	6.2e-189	92.22	chaperone protein dnaJ 49-like OS=Cucumis melo OX=3656 GN=LOC103487463 PE=4 SV=1	[more]
A0A5A7TZX0	6.2e-189	92.22	Chaperone protein dnaJ 49-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A5D3DT80	6.2e-189	92.22	Chaperone protein dnaJ 49-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]

BLAST of Spg022732 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G57340.1	2.7e-120	60.05	Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977)	[more]
AT3G57340.2	2.7e-120	60.05	Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977)	[more]
AT5G05750.1	1.3e-85	59.93	DNAJ heat shock N-terminal domain-containing protein	[more]
AT5G49060.1	8.3e-61	40.72	Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977)	[more]
AT2G20560.1	5.8e-14	36.24	DNAJ heat shock family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 153..173 score: 37.2 coord: 137..152 score: 53.36 coord: 119..137 score: 35.89
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 116..173 e-value: 1.7E-25 score: 100.7
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 117..178 e-value: 4.9E-23 score: 81.0
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 117..181 score: 19.763956
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 117..170 e-value: 2.24986E-20 score: 81.4377
IPR015399	Domain of unknown function DUF1977, DnaJ-like	PFAM	PF09320	DUF1977	coord: 270..340 e-value: 5.2E-8 score: 33.1
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 113..227 e-value: 1.5E-30 score: 108.0
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 112..227
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 54..100
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 63..100
None	No IPR available	PANTHER	PTHR43908:SF9	SUBFAMILY NOT NAMED	coord: 1..360
None	No IPR available	PANTHER	PTHR43908	AT29763P-RELATED	coord: 1..360
IPR018253	DnaJ domain, conserved site	PROSITE	PS00636	DNAJ_1	coord: 158..177

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022732.1	Spg022732.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071218	cellular response to misfolded protein
biological_process	GO:0051085	chaperone cofactor-dependent protein refolding
biological_process	GO:0030433	ubiquitin-dependent ERAD pathway
cellular_component	GO:0005789	endoplasmic reticulum membrane
molecular_function	GO:0030544	Hsp70 protein binding