Spg022584 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022584
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RING-type E3 ubiquitin transferase
Location	scaffold2: 8826009 .. 8826560 (-)
RNA-Seq Expression	Spg022584
Synteny	Spg022584

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGATTCTTCTTCATCTTCTTTTACTCAGCCAACGACTCATCCTGCAAAATCAAGCCTACCCATGTTGTATTACGGCCTTGTGGTCGTCGGAACGGCGGCGATTGTGTTGGTTCTTTACAATTTCATCGTTATCAAGTGGTGCTCCGACAACCGCCACGGCAGAGATTCGCCGGCGCTGGCCGGAAGTTACGCGGAGATGGTGAATTCGAGCCGTAGCTTACAGAGCTCCAGCAGGAATCTGTTGTCCAGCTTCAAATTCAAGAAGGGGTCCACGGCGGCGACGGCGGCGGCGGCGGAGGAAGAAGGGAATGGGAATGAATGTGCGGTTTGTTTGTCGGCGTTTGAGGATGGGGAAGAGGTGAAGAAACTGCCGAGATGCACTCACATGTTCCACGCCCCTTGTATAGACATGTGGCTTTACTCTCACTCCGACTGCCCGCTCTGCCGTGCTCCGGTGGCGGCGCCGGGGCTGTGCCAACGCCGCACGGCGGCGGAGCAGCAGGAGAATTCAGGGCATGGGTTGTTGGAATCCGGCGACATGGTTTAA

mRNA sequence

ATGGGTGATTCTTCTTCATCTTCTTTTACTCAGCCAACGACTCATCCTGCAAAATCAAGCCTACCCATGTTGTATTACGGCCTTGTGGTCGTCGGAACGGCGGCGATTGTGTTGGTTCTTTACAATTTCATCGTTATCAAGTGGTGCTCCGACAACCGCCACGGCAGAGATTCGCCGGCGCTGGCCGGAAGTTACGCGGAGATGGTGAATTCGAGCCGTAGCTTACAGAGCTCCAGCAGGAATCTGTTGTCCAGCTTCAAATTCAAGAAGGGGTCCACGGCGGCGACGGCGGCGGCGGCGGAGGAAGAAGGGAATGGGAATGAATGTGCGGTTTGTTTGTCGGCGTTTGAGGATGGGGAAGAGGTGAAGAAACTGCCGAGATGCACTCACATGTTCCACGCCCCTTGTATAGACATGTGGCTTTACTCTCACTCCGACTGCCCGCTCTGCCGTGCTCCGGTGGCGGCGCCGGGGCTGTGCCAACGCCGCACGGCGGCGGAGCAGCAGGAGAATTCAGGGCATGGGTTGTTGGAATCCGGCGACATGGTTTAA

Coding sequence (CDS)

ATGGGTGATTCTTCTTCATCTTCTTTTACTCAGCCAACGACTCATCCTGCAAAATCAAGCCTACCCATGTTGTATTACGGCCTTGTGGTCGTCGGAACGGCGGCGATTGTGTTGGTTCTTTACAATTTCATCGTTATCAAGTGGTGCTCCGACAACCGCCACGGCAGAGATTCGCCGGCGCTGGCCGGAAGTTACGCGGAGATGGTGAATTCGAGCCGTAGCTTACAGAGCTCCAGCAGGAATCTGTTGTCCAGCTTCAAATTCAAGAAGGGGTCCACGGCGGCGACGGCGGCGGCGGCGGAGGAAGAAGGGAATGGGAATGAATGTGCGGTTTGTTTGTCGGCGTTTGAGGATGGGGAAGAGGTGAAGAAACTGCCGAGATGCACTCACATGTTCCACGCCCCTTGTATAGACATGTGGCTTTACTCTCACTCCGACTGCCCGCTCTGCCGTGCTCCGGTGGCGGCGCCGGGGCTGTGCCAACGCCGCACGGCGGCGGAGCAGCAGGAGAATTCAGGGCATGGGTTGTTGGAATCCGGCGACATGGTTTAA

Protein sequence

MGDSSSSSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPALAGSYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAAAEEEGNGNECAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAAPGLCQRRTAAEQQENSGHGLLESGDMV

Homology

[Close]

BLAST of Spg022584 vs. NCBI nr
Match: KAG6590024.1 (RING-H2 finger protein ATL1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023686.1 RING-H2 finger protein ATL1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 309.3 bits (791), Expect = 2.2e-80
Identity = 154/183 (84.15%), Postives = 164/183 (89.62%), Query Frame = 0


Query: 1   MGDSSSSSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPA 60
           M DS+S+ F QPT+HPAKSSLPMLYYGLVV+GTAAIVLVLYNFIVI WCSDNR GR SPA
Sbjct: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAAAEEEGNGNECAVCLSAFEDGE 120
           LAGSYAEMVNSSRSLQSS+RNLL SFKFKKGS AA  AAAEEEGNGNEC VCLSAFE+GE
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGE 120

Query: 121 EVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAAPGLCQRRTAAEQQENSGHGLLESG 180
           EVKKLPRC HMFHA CIDMWL+SH DCPLCRAPVA PGL +R+TAAEQQ NSGHGL ESG
Sbjct: 121 EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG 180

Query: 181 DMV 184
            +V
Sbjct: 181 GLV 183

[Close]

BLAST of Spg022584 vs. NCBI nr
Match: XP_022968369.1 (RING-H2 finger protein ATL52-like [Cucurbita maxima])

HSP 1 Score: 308.9 bits (790), Expect = 2.9e-80
Identity = 154/186 (82.80%), Postives = 162/186 (87.10%), Query Frame = 0


Query: 1   MGDSSSSSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPA 60
           MGDS+S+   QPT+HPAKSSLPMLYYGLVV+GTA IVLVLYN I+IKWCSDN HG+ SPA
Sbjct: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDNSHGQASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAAAEEEGNGNECAVCLSAFEDGE 120
           LAGSYAEMVNSSRS QSS+RNLLSSFKFKKGSTA   A  EEEG GNECAVCLSAFEDGE
Sbjct: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTAKATATIEEEGIGNECAVCLSAFEDGE 120

Query: 121 EVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAAPGLCQRRT---AAEQQENSGHGLL 180
           EVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAP  AP LCQR T   AAEQQ NSGHGLL
Sbjct: 121 EVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPAMAPALCQRHTAEDAAEQQVNSGHGLL 180

Query: 181 ESGDMV 184
           ES  +V
Sbjct: 181 ESSSLV 186

[Close]

BLAST of Spg022584 vs. NCBI nr
Match: XP_022960602.1 (E3 ubiquitin-protein ligase EL5-like [Cucurbita moschata])

HSP 1 Score: 304.3 bits (778), Expect = 7.1e-79
Identity = 154/183 (84.15%), Postives = 164/183 (89.62%), Query Frame = 0


Query: 1   MGDSSSSSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPA 60
           M DS+S+ F QPT+HPAKSSLPMLYYGLVV+GTAAIVLVLYNFIVI WCSDNR GR SPA
Sbjct: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAAAEEEGNGNECAVCLSAFEDGE 120
           LAGSYAEMVNSSRSLQSS+RNLL SFKFKKGS  A AAAAEEEGNGNEC VCLSAFE+GE
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGS--AAAAAAEEEGNGNECVVCLSAFEEGE 120

Query: 121 EVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAAPGLCQRRTAAEQQENSGHGLLESG 180
           EVKKLPRC HMFHA CIDMWL+SH DCPLCRAPVA PGL +R+TAAEQQ NSGHGL ESG
Sbjct: 121 EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG 180

Query: 181 DMV 184
            +V
Sbjct: 181 GLV 181

[Close]

BLAST of Spg022584 vs. NCBI nr
Match: XP_023516728.1 (E3 ubiquitin-protein ligase EL5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 302.0 bits (772), Expect = 3.5e-78
Identity = 152/184 (82.61%), Postives = 162/184 (88.04%), Query Frame = 0


Query: 1   MGDSSSSSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPA 60
           M DS+S+ F QPT HPAKSSLPMLYYGLVV+GTAAIVLVLYNFIVI WCSDNR GR SPA
Sbjct: 1   MSDSASNYFAQPTNHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAA-AEEEGNGNECAVCLSAFEDG 120
           LAG+YAEMVNSSRSLQSS+RNLL SFKFKKGS AA AAA  EEEGNGNEC VCLSAFE+G
Sbjct: 61  LAGNYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAAAAAVVEEEGNGNECVVCLSAFEEG 120

Query: 121 EEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAAPGLCQRRTAAEQQENSGHGLLES 180
           EEVKKLPRC HMFHA CIDMWL+SH DCPLCRAPVA PGL +R+T AEQQ NSGHGL ES
Sbjct: 121 EEVKKLPRCMHMFHARCIDMWLFSHCDCPLCRAPVAVPGLSKRQTVAEQQGNSGHGLPES 180

Query: 181 GDMV 184
           G +V
Sbjct: 181 GGLV 184

[Close]

BLAST of Spg022584 vs. NCBI nr
Match: XP_022987479.1 (E3 ubiquitin-protein ligase EL5-like [Cucurbita maxima])

HSP 1 Score: 302.0 bits (772), Expect = 3.5e-78
Identity = 152/183 (83.06%), Postives = 162/183 (88.52%), Query Frame = 0


Query: 1   MGDSSSSSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPA 60
           M DS+S+ F+QPT HPAKSSLPMLYYGLVV+GTAAIVLVLYNFIVI WCSDNR GR SPA
Sbjct: 1   MSDSASNHFSQPTNHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAAAEEEGNGNECAVCLSAFEDGE 120
           LAGSYAEMVNSSRSLQSS+RNLL SFKFKKGS    AAA EEEGNGNEC VCLSAFE+GE
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGS---AAAAVEEEGNGNECVVCLSAFEEGE 120

Query: 121 EVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAAPGLCQRRTAAEQQENSGHGLLESG 180
           EVKKLPRC HMFHA CIDMWL+SH DCPLCRAPVA PGL +R+TAAEQQ NSGHGL ESG
Sbjct: 121 EVKKLPRCLHMFHARCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG 180

Query: 181 DMV 184
            +V
Sbjct: 181 GLV 180

[Close]

BLAST of Spg022584 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 6.2e-17
Identity = 58/184 (31.52%), Postives = 85/184 (46.20%), Query Frame = 0


Query: 7   SSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPAL----- 66
           S F    +  + SS   L   L+ + T+A++LV Y  ++ K+C  +     S  L     
Sbjct: 40  SFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHN 99

Query: 67  ----AGSYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAAAEEEGNGNECAVCLSAFE 126
                 S   +  +   L  S    ++ +K+K G             +G++C+VCLS FE
Sbjct: 100 GEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFV---------DGSDCSVCLSEFE 159

Query: 127 DGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRA-------PVAAPGLCQRRTAAEQQE 175
           + E ++ LP+C H FH PCID WL SHS+CPLCRA       P A+ G          Q 
Sbjct: 160 ENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFVTGVNNPTASVGQ-NVSVVVANQS 213

[Close]

BLAST of Spg022584 vs. ExPASy Swiss-Prot
Match: Q9LRB7 (E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5.1 PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 8.1e-17
Identity = 64/172 (37.21%), Postives = 83/172 (48.26%), Query Frame = 0


Query: 1   MGDSSSSSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYN--FIVIK-WCSDNRHGRD 60
           M +SSSSS   P + PA     M   G+  V    IV       F++++ +C + R    
Sbjct: 12  MDESSSSSSPSPVSAPA-GQAAMTAGGIATVAAVLIVFAALTLAFVLLQCYCDERRRAVT 71

Query: 61  SPALAGSYAEMVNSSRSLQSS--------SRNLLSSFKFKKGSTAATAAAA----EEEGN 120
           + + +G         RS               +L S      S +  AAAA    EE+ +
Sbjct: 72  TTSTSGRGRRPRPRRRSGSGGDGGTGGGVDPEVLRSLPVTVYSRSTAAAAAKEEEEEDDD 131

Query: 121 GNECAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAAP 158
           G ECAVCL+  EDGEE + LPRC H FHA C+DMWL SHS CPLCR  V  P
Sbjct: 132 GVECAVCLAELEDGEEARFLPRCGHGFHAECVDMWLGSHSTCPLCRLTVVVP 182

[Close]

BLAST of Spg022584 vs. ExPASy Swiss-Prot
Match: P93823 (RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 6.8e-16
Identity = 59/200 (29.50%), Postives = 96/200 (48.00%), Query Frame = 0


Query: 6   SSSFTQ---PTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSD----------N 65
           S++FT    P T  + ++ P+L   ++ +   A +LV Y   VIK C +           
Sbjct: 23  STTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQIDIFRRRR 82

Query: 66  RHGRDSPALAGSYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAAAEEEGNGNECAVC 125
           R    +P +  S  E+   +R L  S+   +  FKFKK      A   ++  N  EC+VC
Sbjct: 83  RSSDQNPLMIYSPHEV---NRGLDESAIRAIPVFKFKKRD--VVAGEEDQSKNSQECSVC 142

Query: 126 LSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPV-----------AAPGLCQ 182
           L+ F++ E+++ +P C H+FH  CID+WL  +++CPLCR  V           +AP   +
Sbjct: 143 LNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSCEASFTLDLISAPSSPR 202

[Close]

BLAST of Spg022584 vs. ExPASy Swiss-Prot
Match: Q9LSW9 (RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.3e-14
Identity = 56/165 (33.94%), Postives = 78/165 (47.27%), Query Frame = 0


Query: 12  PTTHPAKS---SLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSD-------------NRHG 71
           P  H A S   S P+L   ++ +   A +LV Y   VIK C +              R  
Sbjct: 22  PIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSLSRRRR 81

Query: 72  RDSPALAGSYAEMVNSSRSLQSSSRNLLSSFKFKK-----GSTAATAAAAEEEGNGNECA 131
            D   L     E+   SR L  S    +  FKFKK               EEE    EC+
Sbjct: 82  NDQDPLMVYSPEL--RSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQECS 141

Query: 132 VCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVA 156
           VCLS F+D E+++ +P C+H+FH  CID+WL ++++CPLCR  V+
Sbjct: 142 VCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVS 184

[Close]

BLAST of Spg022584 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 80.5 bits (197), Expect = 2.2e-14
Identity = 57/202 (28.22%), Postives = 88/202 (43.56%), Query Frame = 0


Query: 3   DSSSSSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPALA 62
           DSSSS F+             L   L+ +  +A +LV Y  ++ K+C   RH   S + A
Sbjct: 50  DSSSSDFSP------------LLIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAA 109

Query: 63  GSYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAAAEEEGNG---------------- 122
                 ++S  + Q ++ N         G+T          G+G                
Sbjct: 110 A--INRISSDYTWQGTNNN------NNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRK 169

Query: 123 -------NECAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAAPGL 182
                  ++C+VCLS F++ E ++ LP+C H FH PCID WL SHS+CPLCRA +     
Sbjct: 170 MDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCRAFIVTSSA 229

[Close]

BLAST of Spg022584 vs. ExPASy TrEMBL
Match: A0A6J1HZG2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467629 PE=3 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 1.4e-80
Identity = 154/186 (82.80%), Postives = 162/186 (87.10%), Query Frame = 0


Query: 1   MGDSSSSSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPA 60
           MGDS+S+   QPT+HPAKSSLPMLYYGLVV+GTA IVLVLYN I+IKWCSDN HG+ SPA
Sbjct: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDNSHGQASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAAAEEEGNGNECAVCLSAFEDGE 120
           LAGSYAEMVNSSRS QSS+RNLLSSFKFKKGSTA   A  EEEG GNECAVCLSAFEDGE
Sbjct: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTAKATATIEEEGIGNECAVCLSAFEDGE 120

Query: 121 EVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAAPGLCQRRT---AAEQQENSGHGLL 180
           EVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAP  AP LCQR T   AAEQQ NSGHGLL
Sbjct: 121 EVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPAMAPALCQRHTAEDAAEQQVNSGHGLL 180

Query: 181 ESGDMV 184
           ES  +V
Sbjct: 181 ESSSLV 186

[Close]

BLAST of Spg022584 vs. ExPASy TrEMBL
Match: A0A6J1H7W2 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461337 PE=3 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 3.4e-79
Identity = 154/183 (84.15%), Postives = 164/183 (89.62%), Query Frame = 0


Query: 1   MGDSSSSSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPA 60
           M DS+S+ F QPT+HPAKSSLPMLYYGLVV+GTAAIVLVLYNFIVI WCSDNR GR SPA
Sbjct: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAAAEEEGNGNECAVCLSAFEDGE 120
           LAGSYAEMVNSSRSLQSS+RNLL SFKFKKGS  A AAAAEEEGNGNEC VCLSAFE+GE
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGS--AAAAAAEEEGNGNECVVCLSAFEEGE 120

Query: 121 EVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAAPGLCQRRTAAEQQENSGHGLLESG 180
           EVKKLPRC HMFHA CIDMWL+SH DCPLCRAPVA PGL +R+TAAEQQ NSGHGL ESG
Sbjct: 121 EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG 180

Query: 181 DMV 184
            +V
Sbjct: 181 GLV 181

[Close]

BLAST of Spg022584 vs. ExPASy TrEMBL
Match: A0A6J1JJ05 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485020 PE=3 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.7e-78
Identity = 152/183 (83.06%), Postives = 162/183 (88.52%), Query Frame = 0


Query: 1   MGDSSSSSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPA 60
           M DS+S+ F+QPT HPAKSSLPMLYYGLVV+GTAAIVLVLYNFIVI WCSDNR GR SPA
Sbjct: 1   MSDSASNHFSQPTNHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAAAEEEGNGNECAVCLSAFEDGE 120
           LAGSYAEMVNSSRSLQSS+RNLL SFKFKKGS    AAA EEEGNGNEC VCLSAFE+GE
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGS---AAAAVEEEGNGNECVVCLSAFEEGE 120

Query: 121 EVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAAPGLCQRRTAAEQQENSGHGLLESG 180
           EVKKLPRC HMFHA CIDMWL+SH DCPLCRAPVA PGL +R+TAAEQQ NSGHGL ESG
Sbjct: 121 EVKKLPRCLHMFHARCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG 180

Query: 181 DMV 184
            +V
Sbjct: 181 GLV 180

[Close]

BLAST of Spg022584 vs. ExPASy TrEMBL
Match: A0A6J1D0Z7 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016600 PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 5.5e-77
Identity = 154/181 (85.08%), Postives = 162/181 (89.50%), Query Frame = 0


Query: 4   SSSSSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPALAG 63
           SSS+ FTQPT HPAKSSLPMLYYGLVVVGTAAIVLV+YNFIVI+WCSD R GR SP +A 
Sbjct: 6   SSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNFIVIRWCSD-RRGRGSPGMAR 65

Query: 64  SYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAAAEEEGNGNECAVCLSAFEDGEEVK 123
           SYAEM++SS S QSSSRNLLSSF+FKKG T A AA AEEEGNG ECAVCLSAFEDGEEVK
Sbjct: 66  SYAEMMSSSGSFQSSSRNLLSSFRFKKG-TKAAAATAEEEGNGLECAVCLSAFEDGEEVK 125

Query: 124 KLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVA-APGLCQRRTAAEQQENSGHGLLESGDM 183
           KLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVA APGLCQ  TAAEQ ENSGHGLLESG +
Sbjct: 126 KLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAPGLCQHHTAAEQPENSGHGLLESGGL 184

[Close]

BLAST of Spg022584 vs. ExPASy TrEMBL
Match: A0A6J1EGS5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434176 PE=3 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 5.3e-72
Identity = 140/167 (83.83%), Postives = 148/167 (88.62%), Query Frame = 0


Query: 1   MGDSSSSSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPA 60
           MGDS+S+   QPT+HPAKSSLPMLYYGLVV+GTA IVLVLYN I+IKWCSD+ HG+ S A
Sbjct: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASTA 60

Query: 61  LAGSYAEMVNSSRSLQSSSRNLLSSFKFKKGS--TAATAAAAEEEGNGNECAVCLSAFED 120
           LAGSYAEMVNSSRS QSS+RNLLSSFKFKKGS  TAA  A  EEEG GNECAVCLSAFED
Sbjct: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED 120

Query: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAAPGLCQRRTA 166
           GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPV AP LCQR TA
Sbjct: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTA 167

[Close]

BLAST of Spg022584 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 89.0 bits (219), Expect = 4.4e-18
Identity = 58/184 (31.52%), Postives = 85/184 (46.20%), Query Frame = 0


Query: 7   SSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPAL----- 66
           S F    +  + SS   L   L+ + T+A++LV Y  ++ K+C  +     S  L     
Sbjct: 40  SFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHN 99

Query: 67  ----AGSYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAAAEEEGNGNECAVCLSAFE 126
                 S   +  +   L  S    ++ +K+K G             +G++C+VCLS FE
Sbjct: 100 GEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFV---------DGSDCSVCLSEFE 159

Query: 127 DGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRA-------PVAAPGLCQRRTAAEQQE 175
           + E ++ LP+C H FH PCID WL SHS+CPLCRA       P A+ G          Q 
Sbjct: 160 ENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFVTGVNNPTASVGQ-NVSVVVANQS 213

[Close]

BLAST of Spg022584 vs. TAIR 10
Match: AT1G04360.1 (RING/U-box superfamily protein )

HSP 1 Score: 85.5 bits (210), Expect = 4.8e-17
Identity = 59/200 (29.50%), Postives = 96/200 (48.00%), Query Frame = 0


Query: 6   SSSFTQ---PTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSD----------N 65
           S++FT    P T  + ++ P+L   ++ +   A +LV Y   VIK C +           
Sbjct: 23  STTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQIDIFRRRR 82

Query: 66  RHGRDSPALAGSYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAAAEEEGNGNECAVC 125
           R    +P +  S  E+   +R L  S+   +  FKFKK      A   ++  N  EC+VC
Sbjct: 83  RSSDQNPLMIYSPHEV---NRGLDESAIRAIPVFKFKKRD--VVAGEEDQSKNSQECSVC 142

Query: 126 LSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPV-----------AAPGLCQ 182
           L+ F++ E+++ +P C H+FH  CID+WL  +++CPLCR  V           +AP   +
Sbjct: 143 LNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSCEASFTLDLISAPSSPR 202

[Close]

BLAST of Spg022584 vs. TAIR 10
Match: AT5G43420.1 (RING/U-box superfamily protein )

HSP 1 Score: 81.3 bits (199), Expect = 9.1e-16
Identity = 56/165 (33.94%), Postives = 78/165 (47.27%), Query Frame = 0


Query: 12  PTTHPAKS---SLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSD-------------NRHG 71
           P  H A S   S P+L   ++ +   A +LV Y   VIK C +              R  
Sbjct: 22  PIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSLSRRRR 81

Query: 72  RDSPALAGSYAEMVNSSRSLQSSSRNLLSSFKFKK-----GSTAATAAAAEEEGNGNECA 131
            D   L     E+   SR L  S    +  FKFKK               EEE    EC+
Sbjct: 82  NDQDPLMVYSPEL--RSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQECS 141

Query: 132 VCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVA 156
           VCLS F+D E+++ +P C+H+FH  CID+WL ++++CPLCR  V+
Sbjct: 142 VCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVS 184

[Close]

BLAST of Spg022584 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 80.5 bits (197), Expect = 1.6e-15
Identity = 57/202 (28.22%), Postives = 88/202 (43.56%), Query Frame = 0


Query: 3   DSSSSSFTQPTTHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPALA 62
           DSSSS F+             L   L+ +  +A +LV Y  ++ K+C   RH   S + A
Sbjct: 50  DSSSSDFSP------------LLIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAA 109

Query: 63  GSYAEMVNSSRSLQSSSRNLLSSFKFKKGSTAATAAAAEEEGNG---------------- 122
                 ++S  + Q ++ N         G+T          G+G                
Sbjct: 110 A--INRISSDYTWQGTNNN------NNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRK 169

Query: 123 -------NECAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAAPGL 182
                  ++C+VCLS F++ E ++ LP+C H FH PCID WL SHS+CPLCRA +     
Sbjct: 170 MDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCRAFIVTSSA 229

[Close]

BLAST of Spg022584 vs. TAIR 10
Match: AT2G17450.1 (RING-H2 finger A3A )

HSP 1 Score: 79.3 bits (194), Expect = 3.5e-15
Identity = 50/135 (37.04%), Postives = 66/135 (48.89%), Query Frame = 0


Query: 28  LVVVGTAAIVLVLYNFIVIKWCSDNRHGRDSPALAGSYAEMVNSSRSLQSSSRNLLSSFK 87
           + V G AA+V          W      G DSP+            ++LQS  R+  +   
Sbjct: 40  ICVAGLAAVVR-------CAWLRRFTAGGDSPS-----PNKGLKKKALQSLPRSTFT--- 99

Query: 88  FKKGSTAATAAAAEEEGNGNECAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDC 147
               +  +T+ AA EEG+  ECA+CL+ F DGEE++ LP C H FH  CID WL S S C
Sbjct: 100 ----AAESTSGAAAEEGDSTECAICLTDFADGEEIRVLPLCGHSFHVECIDKWLVSRSSC 154

Query: 148 PLCRAPVAAPGLCQR 163
           P CR  +  P  C R
Sbjct: 160 PSCRR-ILTPVRCDR 154

The following BLAST results are available for this feature:

BLAST of Spg022584 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6590024.1	2.2e-80	84.15	RING-H2 finger protein ATL1, partial [Cucurbita argyrosperma subsp. sororia] >KA...	[more]
XP_022968369.1	2.9e-80	82.80	RING-H2 finger protein ATL52-like [Cucurbita maxima]	[more]
XP_022960602.1	7.1e-79	84.15	E3 ubiquitin-protein ligase EL5-like [Cucurbita moschata]	[more]
XP_023516728.1	3.5e-78	82.61	E3 ubiquitin-protein ligase EL5-like [Cucurbita pepo subsp. pepo]	[more]
XP_022987479.1	3.5e-78	83.06	E3 ubiquitin-protein ligase EL5-like [Cucurbita maxima]	[more]

BLAST of Spg022584 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LF64	6.2e-17	31.52	RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1	[more]
Q9LRB7	8.1e-17	37.21	E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5....	[more]
P93823	6.8e-16	29.50	RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1	[more]
Q9LSW9	1.3e-14	33.94	RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1	[more]
Q9SRQ8	2.2e-14	28.22	RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2	[more]

BLAST of Spg022584 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HZG2	1.4e-80	82.80	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467629 P...	[more]
A0A6J1H7W2	3.4e-79	84.15	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461337...	[more]
A0A6J1JJ05	1.7e-78	83.06	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485020 P...	[more]
A0A6J1D0Z7	5.5e-77	85.08	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101660...	[more]
A0A6J1EGS5	5.3e-72	83.83	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434176...	[more]

BLAST of Spg022584 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17600.1	4.4e-18	31.52	RING/U-box superfamily protein	[more]
AT1G04360.1	4.8e-17	29.50	RING/U-box superfamily protein	[more]
AT5G43420.1	9.1e-16	33.94	RING/U-box superfamily protein	[more]
AT3G03550.1	1.6e-15	28.22	RING/U-box superfamily protein	[more]
AT2G17450.1	3.5e-15	37.04	RING-H2 finger A3A	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 109..150 e-value: 8.7E-6 score: 35.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 107..151 e-value: 4.7E-13 score: 49.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 109..151 score: 12.076194
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 65..157 e-value: 1.5E-21 score: 77.9
None	No IPR available	PANTHER	PTHR45676:SF84	RING-H2 FINGER PROTEIN ATL52-LIKE	coord: 6..178
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 101..156
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 6..178

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022584.1	Spg022584.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane