Spg022497 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg022497
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionENTH domain-containing protein
Locationscaffold2: 6302054 .. 6304425 (+)
RNA-Seq ExpressionSpg022497
SyntenySpg022497
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CATATACATATTATTAGTTTTACATATTAAATACTTACTTCTTGGCAGGGGGGAAATAGATTAGAAAGTATGAAATTACCGAAAAGAAAAAAGAGAAAAGAATATTTCAAAAGAAAAAAGAGAGAGAAAAAAAGAAAGAATAGAAACTTCCAAACGTGGGTGTAAATAGAGCTACGAAGGACTGGTATTTGAATCTTCGTCAGTAAGATATTAAGCCACGTGGCAGCACCTGGAGAACGTACAAGTAGAGTTGAACACGAAGTCGTTTTCAATTTTATAAAAAAAAAAAATTAAAAGCAGGCCGATGAGAATCCATGACCATCACATCCCTTCTGTTACGCTATGGATTTCATTTCTCCGCTCTGAAAAACGCTCTTTCGCTTTCACTGCTTCCTAAAGAATCGCTTTTCCTTCTCTCTTCTGCTTCTTTGCAACACACAGCCTCCTCCTTCATCGCCATTACTGCATTTACTCTTCTACTTACAGGTTACAGGTGAATTTACTAATTTCATTTCATTTCAGTTTCCTGCTCTTTTAATAATCTTTCTGAAAAAGGAGAATCGGTCAGACTCGAGGTGATCGTTTTGTAGGCAATGGCTCAAAGCACCATCAGGAAGGCCATTGGAGCTGTCAAGGATCAAACCAGCATCGGCATCGCCAAAGTCGCCGGAACTGCGGCGCCGGAGCTCGAGGTCTTGGTGGTCAAGGCCACCAGCCACGACGAGGATCCTGCCGACGACAGGTATATCAGGGAGATTGTGAATTTCTGCGCTAATTCGAGTGGATATGTGAGTGCGTGTGTGCTTACCATCTCTAAACGATTGAGTAAGACTCGCGATTGGATCGTCGCGCTCAAGGCGCTAATGGTGGTGCACAGGGTTTTGATCGACGGAAATCCCTCGTTTGGGGAGGAAATTGTGTATGCTACGCGTAAAGGTGTGCGAGTCTTGAATTTATCTGGTTTTAGAGATGAAGCGCATTCGAATTCATGGGATCATTCTAGTTTTGTGAGGTTTTACGCTCTGTATCTCGATGAAATGGTCGAGAATCTGGTTTTTGAGAAGAGAGTGAGAGGGGATAATGATGGTAGCTTTAATCATAGAGACGAAGCTGATTATGGGAGGCAGAGGAGGGGGCAGAGTCCATGGAGGTCAAAGCGACCTGAGAGAGTAGTGGAGGAATTGAATCGATTGCTTCGAATGCTTGATCGAGTTTTAGCTTGTAGGCCGATAGGAATGGCCAAGAGTAGTAGATTAGTAATTGCTGGGCTTGATTTAGTTGCTAGAGAGAGCTTTGGGTTGTGTGTTGAGATTTGTGAGGCATTGGGGCTTTTGTTGGATCACTTCAAAGAAATGGAATATGGTGATTGTCTTAGGGCCTTTGATGCTTGTGCTAATGCTGCAAAGAAGATTGATGAACTTGTCGAATTTTATGCTTGGTGTAAGGATGTGGGGATTGCAAGATCTTCTGAATACCCAGAGGTTCAGAGGATAACTCAAAACCTTTTGGTCACTCTTGGGGGCTTCCTAAAGGAGATGTCAAGAAGGCCAAAGGATAACGATGAGAGGAGAAGGGAGGAGAAGCTGAAACCGCTACCGTCTGAGGAGCCTGAATATGACATGAATGAGATAAAGGCTCTTCCTCCGCCAGAGAAATGTGATCCTGTCCTGCATTCGGTTTCTCAAACTCGATCGCTGAAGGAAGACTTGGTGAACTTGAGAGAGGATGGAATTTCAGCAGATGAGCAAGGGAACAAATTGGCCTTGGATCTTTTCTCTGGACCCCCAATTACAAATTCTTATGGTTCATGGGAAACTTTCTCATCAAACCAGGAGCCTGAAGTGAGTTCAGCGTGGCAGACGCCAGCTGCTGAGGCTGGCAAACCAGATTGGGAACTGACATTGGTTGAAACTAGTAGCAATCTGTCAAAGCAGAAGGCTGATATGGGAGGTGGTTTTGATCCACTGCTACTAAATGGAATGTACGACCAAGGGACGATTAGGCAACATGTCAACGCCGAGCAGTTCGGCGGGAGTTCGAGTAGTGTGGCAGTGGCATTGCAAGGCCAAGGCAAGTCTGTTGCACAAGTGCTGGCACTTCCAGCACCAGATGGGACAGTTCAACCGGTCGGGCATCGGGACCCTTTCGCTGCATCACTCGCGGTGCCACCTCCTTCATATGTGCAGATAGCTGAAATGGAGAAGAAGCAGCAGTTGCTAATGCAGGAGCAGCTTCTGTGGCAGCATTATGGCAGAGATGGAATGCACGGCCAAGTGGATTTGGCAAAACTTGCTGGTGGCTCGGCCTATGTTGGCCTAAATCCTCAGCCAATTATGCCTCATGGCTCAGCCTACTATTATTCTCCCTTTTGA

mRNA sequence

ATGACCATCACATCCCTTCTGTTACGCTATGGATTTCATTTCTCCGCTCTGAAAAACGCTCTTTCGCTTTCACTGCTTCCTAAAGAATCGCTTTTCCTTCTCTCTTCTGCTTCTTTGCAACACACAGCCTCCTCCTTCATCGCCATTACTGCATTTACTCTTCTACTTACAGGTTACAGGAAGGCCATTGGAGCTGTCAAGGATCAAACCAGCATCGGCATCGCCAAAGTCGCCGGAACTGCGGCGCCGGAGCTCGAGGTCTTGGTGGTCAAGGCCACCAGCCACGACGAGGATCCTGCCGACGACAGGTATATCAGGGAGATTGTGAATTTCTGCGCTAATTCGAGTGGATATGTGAGTGCGTGTGTGCTTACCATCTCTAAACGATTGAGTAAGACTCGCGATTGGATCGTCGCGCTCAAGGCGCTAATGGTGGTGCACAGGGTTTTGATCGACGGAAATCCCTCGTTTGGGGAGGAAATTGTGTATGCTACGCGTAAAGGTGTGCGAGTCTTGAATTTATCTGGTTTTAGAGATGAAGCGCATTCGAATTCATGGGATCATTCTAGTTTTGTGAGGTTTTACGCTCTGTATCTCGATGAAATGGTCGAGAATCTGGTTTTTGAGAAGAGAGTGAGAGGGGATAATGATGGTAGCTTTAATCATAGAGACGAAGCTGATTATGGGAGGCAGAGGAGGGGGCAGAGTCCATGGAGGTCAAAGCGACCTGAGAGAGTAGTGGAGGAATTGAATCGATTGCTTCGAATGCTTGATCGAGTTTTAGCTTGTAGGCCGATAGGAATGGCCAAGAGTAGTAGATTAGTAATTGCTGGGCTTGATTTAGTTGCTAGAGAGAGCTTTGGGTTGTGTGTTGAGATTTGTGAGGCATTGGGGCTTTTGTTGGATCACTTCAAAGAAATGGAATATGGTGATTGTCTTAGGGCCTTTGATGCTTGTGCTAATGCTGCAAAGAAGATTGATGAACTTGTCGAATTTTATGCTTGGTGTAAGGATGTGGGGATTGCAAGATCTTCTGAATACCCAGAGGTTCAGAGGATAACTCAAAACCTTTTGGTCACTCTTGGGGGCTTCCTAAAGGAGATGTCAAGAAGGCCAAAGGATAACGATGAGAGGAGAAGGGAGGAGAAGCTGAAACCGCTACCGTCTGAGGAGCCTGAATATGACATGAATGAGATAAAGGCTCTTCCTCCGCCAGAGAAATGTGATCCTGTCCTGCATTCGGTTTCTCAAACTCGATCGCTGAAGGAAGACTTGGTGAACTTGAGAGAGGATGGAATTTCAGCAGATGAGCAAGGGAACAAATTGGCCTTGGATCTTTTCTCTGGACCCCCAATTACAAATTCTTATGGTTCATGGGAAACTTTCTCATCAAACCAGGAGCCTGAAGTGAGTTCAGCGTGGCAGACGCCAGCTGCTGAGGCTGGCAAACCAGATTGGGAACTGACATTGGTTGAAACTAGTAGCAATCTGTCAAAGCAGAAGGCTGATATGGGAGGTGGTTTTGATCCACTGCTACTAAATGGAATGTACGACCAAGGGACGATTAGGCAACATGTCAACGCCGAGCAGTTCGGCGGGAGTTCGAGTAGTGTGGCAGTGGCATTGCAAGGCCAAGGCAAGTCTGTTGCACAAGTGCTGGCACTTCCAGCACCAGATGGGACAGTTCAACCGGTCGGGCATCGGGACCCTTTCGCTGCATCACTCGCGGTGCCACCTCCTTCATATGTGCAGATAGCTGAAATGGAGAAGAAGCAGCAGTTGCTAATGCAGGAGCAGCTTCTGTGGCAGCATTATGGCAGAGATGGAATGCACGGCCAAGTGGATTTGGCAAAACTTGCTGGTGGCTCGGCCTATGTTGGCCTAAATCCTCAGCCAATTATGCCTCATGGCTCAGCCTACTATTATTCTCCCTTTTGA

Coding sequence (CDS)

ATGACCATCACATCCCTTCTGTTACGCTATGGATTTCATTTCTCCGCTCTGAAAAACGCTCTTTCGCTTTCACTGCTTCCTAAAGAATCGCTTTTCCTTCTCTCTTCTGCTTCTTTGCAACACACAGCCTCCTCCTTCATCGCCATTACTGCATTTACTCTTCTACTTACAGGTTACAGGAAGGCCATTGGAGCTGTCAAGGATCAAACCAGCATCGGCATCGCCAAAGTCGCCGGAACTGCGGCGCCGGAGCTCGAGGTCTTGGTGGTCAAGGCCACCAGCCACGACGAGGATCCTGCCGACGACAGGTATATCAGGGAGATTGTGAATTTCTGCGCTAATTCGAGTGGATATGTGAGTGCGTGTGTGCTTACCATCTCTAAACGATTGAGTAAGACTCGCGATTGGATCGTCGCGCTCAAGGCGCTAATGGTGGTGCACAGGGTTTTGATCGACGGAAATCCCTCGTTTGGGGAGGAAATTGTGTATGCTACGCGTAAAGGTGTGCGAGTCTTGAATTTATCTGGTTTTAGAGATGAAGCGCATTCGAATTCATGGGATCATTCTAGTTTTGTGAGGTTTTACGCTCTGTATCTCGATGAAATGGTCGAGAATCTGGTTTTTGAGAAGAGAGTGAGAGGGGATAATGATGGTAGCTTTAATCATAGAGACGAAGCTGATTATGGGAGGCAGAGGAGGGGGCAGAGTCCATGGAGGTCAAAGCGACCTGAGAGAGTAGTGGAGGAATTGAATCGATTGCTTCGAATGCTTGATCGAGTTTTAGCTTGTAGGCCGATAGGAATGGCCAAGAGTAGTAGATTAGTAATTGCTGGGCTTGATTTAGTTGCTAGAGAGAGCTTTGGGTTGTGTGTTGAGATTTGTGAGGCATTGGGGCTTTTGTTGGATCACTTCAAAGAAATGGAATATGGTGATTGTCTTAGGGCCTTTGATGCTTGTGCTAATGCTGCAAAGAAGATTGATGAACTTGTCGAATTTTATGCTTGGTGTAAGGATGTGGGGATTGCAAGATCTTCTGAATACCCAGAGGTTCAGAGGATAACTCAAAACCTTTTGGTCACTCTTGGGGGCTTCCTAAAGGAGATGTCAAGAAGGCCAAAGGATAACGATGAGAGGAGAAGGGAGGAGAAGCTGAAACCGCTACCGTCTGAGGAGCCTGAATATGACATGAATGAGATAAAGGCTCTTCCTCCGCCAGAGAAATGTGATCCTGTCCTGCATTCGGTTTCTCAAACTCGATCGCTGAAGGAAGACTTGGTGAACTTGAGAGAGGATGGAATTTCAGCAGATGAGCAAGGGAACAAATTGGCCTTGGATCTTTTCTCTGGACCCCCAATTACAAATTCTTATGGTTCATGGGAAACTTTCTCATCAAACCAGGAGCCTGAAGTGAGTTCAGCGTGGCAGACGCCAGCTGCTGAGGCTGGCAAACCAGATTGGGAACTGACATTGGTTGAAACTAGTAGCAATCTGTCAAAGCAGAAGGCTGATATGGGAGGTGGTTTTGATCCACTGCTACTAAATGGAATGTACGACCAAGGGACGATTAGGCAACATGTCAACGCCGAGCAGTTCGGCGGGAGTTCGAGTAGTGTGGCAGTGGCATTGCAAGGCCAAGGCAAGTCTGTTGCACAAGTGCTGGCACTTCCAGCACCAGATGGGACAGTTCAACCGGTCGGGCATCGGGACCCTTTCGCTGCATCACTCGCGGTGCCACCTCCTTCATATGTGCAGATAGCTGAAATGGAGAAGAAGCAGCAGTTGCTAATGCAGGAGCAGCTTCTGTGGCAGCATTATGGCAGAGATGGAATGCACGGCCAAGTGGATTTGGCAAAACTTGCTGGTGGCTCGGCCTATGTTGGCCTAAATCCTCAGCCAATTATGCCTCATGGCTCAGCCTACTATTATTCTCCCTTTTGA

Protein sequence

MTITSLLLRYGFHFSALKNALSLSLLPKESLFLLSSASLQHTASSFIAITAFTLLLTGYRKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCANSSGYVSACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFRDEAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGDNDGSFNHRDEADYGRQRRGQSPWRSKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEALGLLLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNLLVTLGGFLKEMSRRPKDNDERRREEKLKPLPSEEPEYDMNEIKALPPPEKCDPVLHSVSQTRSLKEDLVNLREDGISADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTPAAEAGKPDWELTLVETSSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVNAEQFGGSSSSVAVALQGQGKSVAQVLALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQEQLLWQHYGRDGMHGQVDLAKLAGGSAYVGLNPQPIMPHGSAYYYSPF
Homology
BLAST of Spg022497 vs. NCBI nr
Match: XP_022934011.1 (probable clathrin assembly protein At4g32285 [Cucurbita moschata])

HSP 1 Score: 932.6 bits (2409), Expect = 1.9e-267
Identity = 479/586 (81.74%), Postives = 514/586 (87.71%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCANSSGYV 119
           RKA+GAVKDQTSI IAKVAG  A ELEVLVVKATSHDEDPAD RYIREIVN C+ S+GYV
Sbjct: 7   RKALGAVKDQTSISIAKVAGNTASELEVLVVKATSHDEDPADHRYIREIVNLCSTSNGYV 66

Query: 120 SACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFRD 179
           SACV+TISKRLSKTRDWIVALKAL+VVHRVLIDGN SFGEEIVYATRKG+RVL+LS FRD
Sbjct: 67  SACVITISKRLSKTRDWIVALKALVVVHRVLIDGNSSFGEEIVYATRKGIRVLDLSSFRD 126

Query: 180 EAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGDNDGSFNHRDEADYGRQRRGQSPWR 239
           EAHSNSWDHSSFVR YALYLDEM ENLV EKR+RG +D +F+ RDE D  RQRR QSPWR
Sbjct: 127 EAHSNSWDHSSFVRSYALYLDEMAENLVLEKRIRGSDDTNFDRRDEFDNVRQRRRQSPWR 186

Query: 240 SKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEALGL 299
           S RPE VV+EL++LLR+LDR+LACRP GMAKSSRLVI  LDLV  +SFGL VEICEALG 
Sbjct: 187 SMRPESVVDELSQLLRILDRILACRPTGMAKSSRLVITALDLVVNDSFGLYVEICEALGY 246

Query: 300 LLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNLLV 359
           LLD FKEMEYGDCLRAFD CANAAKKIDEL EFY WCKDVGIARSSEYPE QRIT NLLV
Sbjct: 247 LLDRFKEMEYGDCLRAFDVCANAAKKIDELDEFYTWCKDVGIARSSEYPEAQRITCNLLV 306

Query: 360 TLGGFLKEMSRRPKDNDERRREEKLKPLPSEEPEYDMNEIKALPPPEKCDPVLHSVSQTR 419
           TLGGFLKEMSRRPKD +ERRREEK+K LP+EEPE  MNEIKALPP E C P L    Q  
Sbjct: 307 TLGGFLKEMSRRPKDYNERRREEKVKSLPAEEPECYMNEIKALPPLENCSPTL----QPP 366

Query: 420 SLKEDLVNLREDGISADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTPAA 479
           S  EDLVNLREDG+SADEQGNKLAL LFSGPP  N  GSWETFSS+++PEVSSAWQTPAA
Sbjct: 367 SRPEDLVNLREDGVSADEQGNKLALALFSGPP-ANPNGSWETFSSDKQPEVSSAWQTPAA 426

Query: 480 EAGKPDWELTLVETSSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVNAEQFGGSSSSVA 539
           EAGKP+WEL LVET+SNLSKQKADMGGGFDPLLLNGMYD GTIRQH NAEQF GSSSS  
Sbjct: 427 EAGKPEWELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRQHANAEQFDGSSSS-- 486

Query: 540 VALQGQGKSVAQVLALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQEQ 599
           VA++GQ K+ AQVLALPAPDGTV+P+G +DPFAASL VPPPSYVQIAEMEKKQQLLMQEQ
Sbjct: 487 VAMKGQSKTAAQVLALPAPDGTVKPIGDQDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQ 546

Query: 600 LLWQHYGRDGMHGQVDLAKLAGGSAYVGLNPQPIMPHGSAYYYSPF 646
           LLWQ YGRDGMHGQV L KLAGGSAY+G NPQPI P  SAY+Y+PF
Sbjct: 547 LLWQQYGRDGMHGQVGLVKLAGGSAYIGSNPQPIAPQSSAYHYAPF 585

BLAST of Spg022497 vs. NCBI nr
Match: XP_023529315.1 (putative clathrin assembly protein At2g25430 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 931.0 bits (2405), Expect = 5.5e-267
Identity = 479/586 (81.74%), Postives = 514/586 (87.71%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCANSSGYV 119
           RKA+GAVKDQTSI IAKVAG  A ELEVLVVKATSHDEDPAD RYIREIVN C+ S+GYV
Sbjct: 7   RKALGAVKDQTSISIAKVAGNTASELEVLVVKATSHDEDPADHRYIREIVNLCSTSNGYV 66

Query: 120 SACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFRD 179
           SACV+T+SKRLSKTRDWIVALKAL+VVHRVLIDGN SFGEEIVYATRKG+RVL+LSGFRD
Sbjct: 67  SACVITVSKRLSKTRDWIVALKALVVVHRVLIDGNSSFGEEIVYATRKGIRVLDLSGFRD 126

Query: 180 EAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGDNDGSFNHRDEADYGRQRRGQSPWR 239
           EAHSNSWDHSSFVR YALYLDEM ENLV EKR+RG +D +F+ RDE D  RQRR QSPWR
Sbjct: 127 EAHSNSWDHSSFVRSYALYLDEMAENLVLEKRMRGSDDTNFDRRDEFDNVRQRRRQSPWR 186

Query: 240 SKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEALGL 299
           S RPE VV+EL++LLR+LDRVLACRP GMAKSSRLVI  LDLV  +SFGL VEICE LG 
Sbjct: 187 SMRPESVVDELSQLLRILDRVLACRPTGMAKSSRLVITALDLVVNDSFGLYVEICEGLGY 246

Query: 300 LLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNLLV 359
           LLD FKEMEYGDCLRAFD CANAAKKIDEL EFY WCKDVGIARSSEYPE QRIT NLLV
Sbjct: 247 LLDRFKEMEYGDCLRAFDVCANAAKKIDELDEFYTWCKDVGIARSSEYPEAQRITCNLLV 306

Query: 360 TLGGFLKEMSRRPKDNDERRREEKLKPLPSEEPEYDMNEIKALPPPEKCDPVLHSVSQTR 419
           TLGGFLKEMSRRPKD +ERRREEK+K LP+EEPE  MNEIKALPP E C P L    Q  
Sbjct: 307 TLGGFLKEMSRRPKDYNERRREEKVKSLPAEEPECYMNEIKALPPLENCSPTL----QPP 366

Query: 420 SLKEDLVNLREDGISADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTPAA 479
           S   DLVNLREDG+SADEQGNKLAL LFSGPP  N  GSWETFSS+++PEVSSAWQTPAA
Sbjct: 367 SRPIDLVNLREDGVSADEQGNKLALALFSGPP-ANPNGSWETFSSDKQPEVSSAWQTPAA 426

Query: 480 EAGKPDWELTLVETSSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVNAEQFGGSSSSVA 539
           EAGKP+WEL LVET+SNLSKQKADMGGGFDPLLLNGMYD GTIR+H NAEQFGGSSSS  
Sbjct: 427 EAGKPEWELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRKHANAEQFGGSSSS-- 486

Query: 540 VALQGQGKSVAQVLALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQEQ 599
           VAL+GQGK+ AQVLALPAPDGTV+P+G +DPFAASL VPPPSYVQIAEME KQQLLMQEQ
Sbjct: 487 VALKGQGKTAAQVLALPAPDGTVKPIGDQDPFAASLTVPPPSYVQIAEMEMKQQLLMQEQ 546

Query: 600 LLWQHYGRDGMHGQVDLAKLAGGSAYVGLNPQPIMPHGSAYYYSPF 646
           LLWQ YGRDGMHGQV L KLAGGSAY+G NPQPI P  SAY+Y+PF
Sbjct: 547 LLWQQYGRDGMHGQVGLVKLAGGSAYIGPNPQPIAPQSSAYHYAPF 585

BLAST of Spg022497 vs. NCBI nr
Match: KAG6587890.1 (putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 929.9 bits (2402), Expect = 1.2e-266
Identity = 478/586 (81.57%), Postives = 513/586 (87.54%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCANSSGYV 119
           RKA+GAVKDQTSI IAKVAG  A ELEVLVVKATSHDEDPA  RYIREIVN C+ S+GYV
Sbjct: 7   RKALGAVKDQTSISIAKVAGNTASELEVLVVKATSHDEDPAGHRYIREIVNLCSTSNGYV 66

Query: 120 SACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFRD 179
           SACV+TISKRLSKTRDWIVALKAL+VVHRVLIDGN SFGEEIVYATRKG+RVL+LS FRD
Sbjct: 67  SACVITISKRLSKTRDWIVALKALVVVHRVLIDGNSSFGEEIVYATRKGIRVLDLSSFRD 126

Query: 180 EAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGDNDGSFNHRDEADYGRQRRGQSPWR 239
           EAHSNSWDHSSFVR YALYLDEM ENLV EKR+RG +D +F+ RDE D  RQRR QSPWR
Sbjct: 127 EAHSNSWDHSSFVRSYALYLDEMAENLVLEKRIRGSDDTNFDRRDEFDNVRQRRRQSPWR 186

Query: 240 SKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEALGL 299
           S RPE VV+EL++LLR+LDR+LACRP GMAKSSRLVI  LDLV  +SFGL VEICEALG 
Sbjct: 187 SMRPESVVDELSQLLRILDRILACRPTGMAKSSRLVITALDLVVNDSFGLYVEICEALGY 246

Query: 300 LLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNLLV 359
           LLD FKEMEYGDCLRAFD CANAAKKIDEL EFY WC DVGIARSSEYPE QRIT NLLV
Sbjct: 247 LLDRFKEMEYGDCLRAFDVCANAAKKIDELDEFYTWCTDVGIARSSEYPEAQRITCNLLV 306

Query: 360 TLGGFLKEMSRRPKDNDERRREEKLKPLPSEEPEYDMNEIKALPPPEKCDPVLHSVSQTR 419
           TLGGFLKEMSRRPKD +ERRREEK+K LP+EEPE  MNEIKALPP E C P L    Q  
Sbjct: 307 TLGGFLKEMSRRPKDYNERRREEKVKSLPAEEPECYMNEIKALPPLENCSPTL----QPP 366

Query: 420 SLKEDLVNLREDGISADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTPAA 479
           S  EDLVNLREDG+SADEQGNKLAL LFSGPP  N  GSWETFSS+++PEVSSAWQTPAA
Sbjct: 367 SRPEDLVNLREDGVSADEQGNKLALALFSGPP-ANPNGSWETFSSDKQPEVSSAWQTPAA 426

Query: 480 EAGKPDWELTLVETSSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVNAEQFGGSSSSVA 539
           EAGKP+WEL LVET+SNLSKQKADMGGGFDPLLLNGMYD GTIRQH NAEQF GSSSS  
Sbjct: 427 EAGKPEWELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRQHANAEQFDGSSSS-- 486

Query: 540 VALQGQGKSVAQVLALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQEQ 599
           VA++GQGK+ AQVLALPAPDGTV+P+G +DPFAASL VPPPSYVQIAEMEKKQQLLMQEQ
Sbjct: 487 VAMKGQGKTAAQVLALPAPDGTVKPIGDQDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQ 546

Query: 600 LLWQHYGRDGMHGQVDLAKLAGGSAYVGLNPQPIMPHGSAYYYSPF 646
           LLWQ YGRDGMHGQV L KLAGGSAY+G NPQPI P  SAY+Y+PF
Sbjct: 547 LLWQQYGRDGMHGQVGLVKLAGGSAYIGSNPQPIAPQSSAYHYAPF 585

BLAST of Spg022497 vs. NCBI nr
Match: XP_022145098.1 (probable clathrin assembly protein At4g32285 [Momordica charantia])

HSP 1 Score: 924.5 bits (2388), Expect = 5.1e-265
Identity = 476/588 (80.95%), Postives = 523/588 (88.95%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCANSSGYV 119
           RKAIGAVKDQTSI IAKVAG  APELEVLVVKATSHDEDP DDRYI EIVN C++S+GYV
Sbjct: 7   RKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCSHSNGYV 66

Query: 120 SACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFRD 179
           SACV+TISKRL+KTRDWIVALKALMVVHRVLIDG+PSFGEEIVYATR+G+RVLNLSGFRD
Sbjct: 67  SACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLNLSGFRD 126

Query: 180 EAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGD-NDGSFNHRDEADYGRQRRGQSPW 239
           EAHSNSWDHS FVR YALYLD M+E+LV EK+VRG  +DG   + DE+DYGRQRR QSPW
Sbjct: 127 EAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDG---NSDESDYGRQRRRQSPW 186

Query: 240 RSKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEALG 299
           RS RPERV++E ++LLRMLDRVLACRP+GMAKSSRLVIA + L  +ESFGL +EICEALG
Sbjct: 187 RSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEICEALG 246

Query: 300 LLLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNLL 359
           +LLDHFKEMEYGDCLRAFD   NAAKKID+L+EFYAWCKD+GIARSSEYPEVQRIT NLL
Sbjct: 247 VLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRITHNLL 306

Query: 360 VTLGGFLKEMSRRPKDNDERRREEKLKPLPSEEPEYDMNEIKALPPPEKCDPVLHSV-SQ 419
            TLG FLKEM RRPKD +ERRREEKLK  P+++PE DMNE+KALPPP+  DP LHSV  Q
Sbjct: 307 GTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSLHSVLPQ 366

Query: 420 TRSLKEDLVNLREDGISADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTP 479
           TR  KEDLVNLRED +SADEQGNKLAL LFSGPPITN  GSWETFSSNQ+PEVSSAW+TP
Sbjct: 367 TRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSAWKTP 426

Query: 480 AAEAGKPDWELTLVETSSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVNAEQFGGSSSS 539
           AAEAGK DWEL LVET+SNLSKQKADMGGGFDPLLLN MYDQGTIR+HVNAEQFGGSSSS
Sbjct: 427 AAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQFGGSSSS 486

Query: 540 VAVALQGQGKSVAQVLALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQ 599
           VA+ +QG+    AQVLALPAPDGTV PVGHRDPFAASL VPPPSYVQIAEMEKKQQLL+Q
Sbjct: 487 VALQVQGK-TXAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQLLVQ 546

Query: 600 EQLLWQHYGRDGMHGQVDLAKLAGGSAYVGLNPQPIMPHGSAYYYSPF 646
           EQL+WQ YGR+GMHGQV LAK+ GGSA+V +    IMPH SAYYY+PF
Sbjct: 547 EQLVWQQYGREGMHGQVGLAKIGGGSAHVNV---AIMPHASAYYYAPF 587

BLAST of Spg022497 vs. NCBI nr
Match: XP_038879765.1 (putative clathrin assembly protein At2g25430 [Benincasa hispida])

HSP 1 Score: 920.2 bits (2377), Expect = 9.7e-264
Identity = 468/587 (79.73%), Postives = 515/587 (87.73%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCAN-SSGY 119
           RKAIG +KDQT+I IAKVAG   PEL+VLVVKATSHDEDPADDRYIREIVN C+N S+GY
Sbjct: 7   RKAIGVMKDQTTISIAKVAGNMTPELDVLVVKATSHDEDPADDRYIREIVNLCSNYSNGY 66

Query: 120 VSACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFR 179
           V+ACV+TIS+RLSKTRDWIVALK+L+VVHR+LIDG+PSFGEEIVYATRKG+RVLNLSGFR
Sbjct: 67  VNACVVTISRRLSKTRDWIVALKSLIVVHRILIDGHPSFGEEIVYATRKGMRVLNLSGFR 126

Query: 180 DEAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGDNDGSFNHRDEADYGRQRRGQSPW 239
           DEAHSNSWDHSSF+RFYALYLDE++ENLV EKR++G         DE+ Y  + R QSPW
Sbjct: 127 DEAHSNSWDHSSFLRFYALYLDELIENLVLEKRLKGG--------DESCY--RSRKQSPW 186

Query: 240 RSKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEALG 299
           RS RPERVVEELN+LLRMLDRVLACRP GMAK+SRLVI  L+L+ +ESFGL VEIC+ALG
Sbjct: 187 RSMRPERVVEELNQLLRMLDRVLACRPAGMAKNSRLVITALNLIIKESFGLYVEICDALG 246

Query: 300 LLLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNLL 359
           +LLDHFKEMEY DCLRAFD CANAAK I++LVEFY WCKDVGIARSSEYPEVQ IT NLL
Sbjct: 247 VLLDHFKEMEYVDCLRAFDVCANAAKNINDLVEFYGWCKDVGIARSSEYPEVQMITHNLL 306

Query: 360 VTLGGFLKEMSRRPKDNDERRREEKLKPLPSEEPEYDMNEIKALPPPEKCDPVLHSVSQT 419
           +TLGGFLKEMSRRPKD DE R EE++KPLP  E  YDMNEIKALP  E C PV  S+ +T
Sbjct: 307 ITLGGFLKEMSRRPKDFDEMRTEEEMKPLPVNESGYDMNEIKALPLLENCAPVPQSILRT 366

Query: 420 RSLKEDLVNLREDGISADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTPA 479
           +S KEDLVNLREDG+SADEQGNKLAL LFSGPPITN  GSWETFSSN EPEVSSAWQTPA
Sbjct: 367 QSPKEDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPEVSSAWQTPA 426

Query: 480 AEAGKPDWELTLVETSSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVNAEQFGGSSSSV 539
           AE GKPDWELTLVET+S+LSKQK DMGG FDPLLLNGMYDQ T+R+HVNA QFGGSSSSV
Sbjct: 427 AEFGKPDWELTLVETASHLSKQKPDMGGCFDPLLLNGMYDQETVRRHVNAVQFGGSSSSV 486

Query: 540 AVALQGQGKSVAQVLALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQE 599
           AVALQGQG + AQVLALPAPDGT +P+G+RDPFAASL VPPPSYVQIAEMEKKQ+LL QE
Sbjct: 487 AVALQGQGNAAAQVLALPAPDGTAEPIGYRDPFAASLTVPPPSYVQIAEMEKKQELLTQE 546

Query: 600 QLLWQHYGRDGMHGQVDLAKLAGGSAYVGLNPQPIMPHGSAYYYSPF 646
           QLLW+ YGRDGM GQ  L KL GGSAYV LNPQ IMPHGSAYYY+ F
Sbjct: 547 QLLWKQYGRDGMQGQGGLGKLGGGSAYVDLNPQQIMPHGSAYYYASF 583

BLAST of Spg022497 vs. ExPASy Swiss-Prot
Match: Q8S9J8 (Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=At4g32285 PE=1 SV=2)

HSP 1 Score: 621.3 bits (1601), Expect = 1.2e-176
Identity = 340/634 (53.63%), Postives = 424/634 (66.88%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCANSSGYV 119
           RKAIG VKDQTSIGIAKVA   AP+LEV +VKATSHD+D + D+YIREI++  + S GYV
Sbjct: 6   RKAIGVVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRGYV 65

Query: 120 SACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFRD 179
            ACV ++S+RL KTRDWIVALKALM+VHR+L +G+P F EEI+YATR+G R+LN+S FRD
Sbjct: 66  HACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDFRD 125

Query: 180 EAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGDNDGSFNHRDEADYG---------- 239
           EAHS+SWDHS+FVR YA YLD+ +E  +FE+R R     S +H+   D G          
Sbjct: 126 EAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDDFRS 185

Query: 240 --------------------------------RQRRGQSPWRSKRPERVVEELNRLLRML 299
                                            +++  +P R   PER+  ++  L R+L
Sbjct: 186 PPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFGKMGHLQRLL 245

Query: 300 DRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEALGLLLDHFKEMEYGDCLRAFD 359
           DR L+CRP G+AK+SR+++  +  V +ESF L  +ICE L +LLD F +MEY DC++AFD
Sbjct: 246 DRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTDCVKAFD 305

Query: 360 ACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNLLVTLGGFLKEMSRRPKDNDE 419
           A A+AAK+IDEL+ FY WCKD G+ARSSEYPEVQRIT  LL TL  F+++ ++R K  + 
Sbjct: 306 AYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKRAKSPER 365

Query: 420 RRREEKLKPLPSEEPEYDMNEIKALPPPEK----CDPVLHSVSQTRSLKEDLVNLREDGI 479
           +  E    P P  E   DMNEIKALPPPE       P      Q   + +DLVNLRED +
Sbjct: 366 KEIEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPAPEPKPQQPQVTDDLVNLREDDV 425

Query: 480 SADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTPAAEAGKPDWELTLVET 539
           S D+QGNK AL LF+GPP  N  G WE FSS+    V+SAWQ PAAE GK DWEL LVET
Sbjct: 426 SGDDQGNKFALALFAGPPANN--GKWEAFSSDN--NVTSAWQNPAAELGKADWELALVET 485

Query: 540 SSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVN-AEQFGGSSSSVAVALQGQGKSVAQV 599
           +SNL  QKA MGGG DPLLLNGMYDQG +RQHV+ +E  GGSSSSVA+ L G+  S   +
Sbjct: 486 ASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELTGGSSSSVALPLPGKVNS--HI 545

Query: 600 LALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQEQLLWQHYGRDGMHG 644
           LALPAPDGTVQ V ++DPFAASL +PPPSYVQ+AEM+KKQ LL QEQ LWQ Y ++GM G
Sbjct: 546 LALPAPDGTVQKV-NQDPFAASLTIPPPSYVQMAEMDKKQYLLTQEQQLWQQYQQEGMRG 605

BLAST of Spg022497 vs. ExPASy Swiss-Prot
Match: Q8LF20 (Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=At2g25430 PE=1 SV=2)

HSP 1 Score: 616.3 bits (1588), Expect = 3.9e-175
Identity = 353/654 (53.98%), Postives = 440/654 (67.28%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCANSSGYV 119
           RKAIGAVKDQTSIGIAKVA   AP+LEV +VKATSHD+DPA ++YIREI+N  + S GY+
Sbjct: 6   RKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTSLSRGYI 65

Query: 120 SACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFRD 179
            ACV ++S+RLSKTRDW+VALKALM+VHR+L +G+P F EEI+Y+TR+G R+LN+S FRD
Sbjct: 66  LACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRMLNMSDFRD 125

Query: 180 EAHSNSWDHSSFVRFYALYLDEMVENLVFEKR---------------------------- 239
           EAHS+SWDHS+FVR YA YLD+ +E  +FE++                            
Sbjct: 126 EAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDDRYGRGRDDF 185

Query: 240 -------------------VRGDNDG-------SFNHRDEADYG--------RQRRGQSP 299
                               RGDN+G       S ++ D  + G         +++  +P
Sbjct: 186 RSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGGGRDEKKVVTP 245

Query: 300 WRSKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEAL 359
            R   PER+  ++  L R+LDR L+ RP G+AK+SR+++  L  V RESF L  +ICE L
Sbjct: 246 LREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFKLYADICEVL 305

Query: 360 GLLLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNL 419
            +LLD F +MEY DC++AFDA A+AAK+IDEL+ FY WCK+ G+ARSSEYPEVQRIT  L
Sbjct: 306 AVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYPEVQRITSKL 365

Query: 420 LVTLGGFLKEMSRRPKDNDERRREEKLKPLPSEEPEYDMNEIKALPPPEKCDPVLHSVSQ 479
           L TL  F+++ ++R K  + +  E     +  EEPE DMNEIKALPPPE   P      +
Sbjct: 366 LETLEEFVRDRAKRGKSPERKEIEAPPPVVEEEEPEPDMNEIKALPPPENYTPPPPPEPE 425

Query: 480 TRSLK----EDLVNLREDGISADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSA 539
            +  K    EDLVNLRED ++AD+QGNK AL LF+GPP  N  G WE FSSN    V+SA
Sbjct: 426 PQPEKPQFTEDLVNLREDEVTADDQGNKFALALFAGPPGNN--GKWEAFSSN---GVTSA 485

Query: 540 WQTPAAEAGKPDWELTLVETSSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVNAEQF-G 599
           WQ PAAE GK DWEL LVET+SNL KQ A +GGGFD LLLNGMYDQG +RQHV+  Q  G
Sbjct: 486 WQNPAAEPGKADWELALVETTSNLEKQTAALGGGFDNLLLNGMYDQGMVRQHVSTSQLTG 545

Query: 600 GSSSSVAVALQGQGKSVAQVLALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQ 644
           GS+SSVA+ L   GK+  QVLALPAPDGTV+ V ++DPFAASL +PPPSYVQ+AEMEKKQ
Sbjct: 546 GSASSVALPL--PGKTNNQVLALPAPDGTVEKV-NQDPFAASLTIPPPSYVQMAEMEKKQ 605

BLAST of Spg022497 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 3.9e-114
Identity = 242/602 (40.20%), Postives = 364/602 (60.47%), Query Frame = 0

Query: 59  YRKAIGAVKDQTSIGIAKVAGTAA--PELEVLVVKATSHDEDPADDRYIREIVNFCANSS 118
           +++AIGAVKDQTS+G+AKV G +A   EL+V +VKAT H+E PA+++YIREI++  + S 
Sbjct: 6   FKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSLTSYSR 65

Query: 119 GYVSACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSG 178
            Y++ACV T+S+RL+KT+ W VALK L+++ R+L +G+ ++ +EI +ATR+G R+LN+S 
Sbjct: 66  SYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRLLNMSD 125

Query: 179 FRDEAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGDNDGSFNHRDEADYGRQRRG-- 238
           FRD + SNSWD+S+FVR YALYLDE ++   F  + R    G +    EAD   Q +   
Sbjct: 126 FRDVSRSNSWDYSAFVRTYALYLDERLD---FRMQARHGKRGVYCVGGEADEEEQDQAAA 185

Query: 239 ---------QSPWRSKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVARE 298
                      P    + E++   +  L ++LDR LACRP G A+++R+VI  L  + +E
Sbjct: 186 DLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPIVKE 245

Query: 299 SFGLCVEICEALGLLLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARSS 358
           SF +  ++ E +G+L++ F E++  D ++ +D     +K+ +EL +FY+WCK++GIARSS
Sbjct: 246 SFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIARSS 305

Query: 359 EYPEVQRITQNLLVTLGGFLKE-------------MSRRPKDNDERRREEKLKPLPSEEP 418
           EYPE+++ITQ  L  +  F+++              S   +D+DE R EE        E 
Sbjct: 306 EYPEIEKITQKKLDLMDEFIRDKSALEHTKQSKSVKSEADEDDDEARTEE------VNEE 365

Query: 419 EYDMNEIKAL--PPPEKCDPVLHS--------VSQTRSLKEDLVNL-REDGISADEQGNK 478
           + DMN IKAL  PPP++ D V           + + +    DL++L   +G  A + G+ 
Sbjct: 366 QEDMNAIKALPEPPPKEEDDVKPEEEAKEEVIIEKKQEEMGDLLDLGNTNGGEAGQAGDS 425

Query: 479 LALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTPAAEAGKPDWELTLVETSSNLSKQK 538
           LAL LF GP           ++S    E    W+  A +    DWE  LV+T++NLS QK
Sbjct: 426 LALALFDGP-----------YASGSGSESGPGWE--AFKDDSADWETALVQTATNLSGQK 485

Query: 539 ADMGGGFDPLLLNGMYDQGTIRQHV-NAEQFGGSSSSVAVALQGQGKSVAQVLALPAPD- 598
           +++GGGFD LLLNGMY  G +   V  +  +G S S+ ++A    G+  A +LALPAP  
Sbjct: 486 SELGGGFDMLLLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATMLALPAPST 545

Query: 599 -----GTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQEQLLWQHYGRDGMHGQV 617
                G +      DPFAASL V PP+YVQ+ +MEKKQ++LM+EQ++W  Y RDG  G +
Sbjct: 546 ANGNAGNINSPVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSRDGRQGHM 585

BLAST of Spg022497 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 407.1 bits (1045), Expect = 3.6e-112
Identity = 239/602 (39.70%), Postives = 359/602 (59.63%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAG--TAAPELEVLVVKATSHDEDPADDRYIREIVNFCANSSG 119
           ++AIGAVKDQTS+G+AKV G  ++  ELE+ VVKAT HD+ PA+D+YIREI+   + S  
Sbjct: 7   KRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCLTSYSRN 66

Query: 120 YVSACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGF 179
           YVSACV T+S+RL+KT++W VALK L+++ R+L DG+ ++ +EI +ATR+G R+LN+S F
Sbjct: 67  YVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRLLNMSDF 126

Query: 180 RDEAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVR----------GDNDGSFNHRDEAD 239
           RD + S+SWD+S+FVR YALYLDE ++  +  +R +          GD+    +HR  ++
Sbjct: 127 RDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEEDDHRGTSN 186

Query: 240 YGRQRR---GQSPWRSKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVAR 299
             R +       P    + E++   +  L ++LDR LACRP G AK++R+VI  +  + +
Sbjct: 187 DIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIVAMYPIVK 246

Query: 300 ESFGLCVEICEALGLLLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARS 359
           ESF L   I E +G+L++ F E++  D ++ ++     +K+ DEL  FY WCK++ +ARS
Sbjct: 247 ESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCKNMAVARS 306

Query: 360 SEYPEVQRITQNLLVTLGGFLKEMS-------RRPKDNDERRREEKLKPLPSEEPEYDMN 419
           SEYPE+++ITQ  L  +  F+++ S       +       +  EE+ K    +E + D+N
Sbjct: 307 SEYPELEKITQKKLDLMDEFIRDKSALAAQTTKSSSKRSNKSEEEESKTEYIQENQEDLN 366

Query: 420 EIKALPPPEKCDPV-----------LHSVSQTRSLKEDLVNLRED-GISADEQGNKLALD 479
            IKALP PE+ +             +  V   +  + DL++L ++ G++A   G+ LAL 
Sbjct: 367 SIKALPAPEQKEEEEEEEKMETKKDVEEVVSRQDQEGDLLDLTDEAGVTAGTVGDSLALA 426

Query: 480 LFSGPPITNSYG--SWETFSSNQEPEVSSAWQTPAAEAGKPDWELTLVETSSNLSKQKAD 539
           LF G   T S     WE F+ N                   DWE  LV +++ LS QK++
Sbjct: 427 LFDGVVGTESASGPGWEAFNDN-----------------SADWETDLVRSATRLSGQKSE 486

Query: 540 MGGGFDPLLLNGMYDQGTIRQHV-NAEQFGGSSSSVAVALQGQGKSVAQVLALPAPDGTV 599
           +GGGFD LLL+GMY  G +   V  +  +G S S+ +VA    G   A +LALPAP  T 
Sbjct: 487 LGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSASSVAFGSAGSPAASMLALPAPPPTA 546

Query: 600 QPVGHR------DPFAASLAVPPPSYVQIAEMEKKQQLLMQEQLLWQHYGRDGMHGQVDL 619
              G+R      DPFAASL V PP+YVQ+ +MEKKQ+LLM+EQ++W  Y R G  G ++ 
Sbjct: 547 N--GNRNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSGRQGHMNF 589

BLAST of Spg022497 vs. ExPASy Swiss-Prot
Match: Q9ZVN6 (Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE=1 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 5.3e-63
Identity = 135/328 (41.16%), Postives = 202/328 (61.59%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAA-----PELEVLVVKATSHDED-PADDRYIREIVNFCA 119
           +KAIGAVKDQTSI +AKVA  A        LEV ++KATSHDE+ P DDR + EI+   +
Sbjct: 6   KKAIGAVKDQTSISLAKVANGATGGGDLTTLEVAILKATSHDEEVPIDDRLVTEILGIIS 65

Query: 120 NSSGYVSACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLN 179
           +   + ++C   I +R+ +TR+WIVALK+L++V R+  DG+P F  E+++A ++G ++LN
Sbjct: 66  SKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKRGAKILN 125

Query: 180 LSGFRDEAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGDNDGSFNHRDEADYGRQRR 239
           LS FRD+++S  WD ++FVR +ALYLDE ++  +  K  R   +     R   +   + R
Sbjct: 126 LSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKLQRRYTNREQTGRISTNSTTRSR 185

Query: 240 ---------GQSPWRSKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVAR 299
                     +   R  +P  +++++    ++LDR +A RP G AK++RLV   L  V +
Sbjct: 186 FNPKAGIKSHEPAVRDMKPVMLLDKITYWQKLLDRAIATRPTGDAKANRLVKMSLYAVMQ 245

Query: 300 ESFGLCVEICEALGLLLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARS 359
           ESF L  +I + L LLLD F  ++Y  C+ AF AC  A+K+ +EL  FY   K +GI R+
Sbjct: 246 ESFDLYRDISDGLALLLDSFFHLQYQSCINAFQACVRASKQFEELNAFYDLSKSIGIGRT 305

Query: 360 SEYPEVQRITQNLLVTLGGFLKEMSRRP 373
           SEYP +Q+I+  LL TL  FLK+ S  P
Sbjct: 306 SEYPSIQKISLELLETLQEFLKDQSSFP 333

BLAST of Spg022497 vs. ExPASy TrEMBL
Match: A0A6J1F0M3 (probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LOC111441244 PE=4 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 9.1e-268
Identity = 479/586 (81.74%), Postives = 514/586 (87.71%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCANSSGYV 119
           RKA+GAVKDQTSI IAKVAG  A ELEVLVVKATSHDEDPAD RYIREIVN C+ S+GYV
Sbjct: 7   RKALGAVKDQTSISIAKVAGNTASELEVLVVKATSHDEDPADHRYIREIVNLCSTSNGYV 66

Query: 120 SACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFRD 179
           SACV+TISKRLSKTRDWIVALKAL+VVHRVLIDGN SFGEEIVYATRKG+RVL+LS FRD
Sbjct: 67  SACVITISKRLSKTRDWIVALKALVVVHRVLIDGNSSFGEEIVYATRKGIRVLDLSSFRD 126

Query: 180 EAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGDNDGSFNHRDEADYGRQRRGQSPWR 239
           EAHSNSWDHSSFVR YALYLDEM ENLV EKR+RG +D +F+ RDE D  RQRR QSPWR
Sbjct: 127 EAHSNSWDHSSFVRSYALYLDEMAENLVLEKRIRGSDDTNFDRRDEFDNVRQRRRQSPWR 186

Query: 240 SKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEALGL 299
           S RPE VV+EL++LLR+LDR+LACRP GMAKSSRLVI  LDLV  +SFGL VEICEALG 
Sbjct: 187 SMRPESVVDELSQLLRILDRILACRPTGMAKSSRLVITALDLVVNDSFGLYVEICEALGY 246

Query: 300 LLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNLLV 359
           LLD FKEMEYGDCLRAFD CANAAKKIDEL EFY WCKDVGIARSSEYPE QRIT NLLV
Sbjct: 247 LLDRFKEMEYGDCLRAFDVCANAAKKIDELDEFYTWCKDVGIARSSEYPEAQRITCNLLV 306

Query: 360 TLGGFLKEMSRRPKDNDERRREEKLKPLPSEEPEYDMNEIKALPPPEKCDPVLHSVSQTR 419
           TLGGFLKEMSRRPKD +ERRREEK+K LP+EEPE  MNEIKALPP E C P L    Q  
Sbjct: 307 TLGGFLKEMSRRPKDYNERRREEKVKSLPAEEPECYMNEIKALPPLENCSPTL----QPP 366

Query: 420 SLKEDLVNLREDGISADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTPAA 479
           S  EDLVNLREDG+SADEQGNKLAL LFSGPP  N  GSWETFSS+++PEVSSAWQTPAA
Sbjct: 367 SRPEDLVNLREDGVSADEQGNKLALALFSGPP-ANPNGSWETFSSDKQPEVSSAWQTPAA 426

Query: 480 EAGKPDWELTLVETSSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVNAEQFGGSSSSVA 539
           EAGKP+WEL LVET+SNLSKQKADMGGGFDPLLLNGMYD GTIRQH NAEQF GSSSS  
Sbjct: 427 EAGKPEWELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRQHANAEQFDGSSSS-- 486

Query: 540 VALQGQGKSVAQVLALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQEQ 599
           VA++GQ K+ AQVLALPAPDGTV+P+G +DPFAASL VPPPSYVQIAEMEKKQQLLMQEQ
Sbjct: 487 VAMKGQSKTAAQVLALPAPDGTVKPIGDQDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQ 546

Query: 600 LLWQHYGRDGMHGQVDLAKLAGGSAYVGLNPQPIMPHGSAYYYSPF 646
           LLWQ YGRDGMHGQV L KLAGGSAY+G NPQPI P  SAY+Y+PF
Sbjct: 547 LLWQQYGRDGMHGQVGLVKLAGGSAYIGSNPQPIAPQSSAYHYAPF 585

BLAST of Spg022497 vs. ExPASy TrEMBL
Match: A0A6J1CV16 (probable clathrin assembly protein At4g32285 OS=Momordica charantia OX=3673 GN=LOC111014605 PE=4 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 2.5e-265
Identity = 476/588 (80.95%), Postives = 523/588 (88.95%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCANSSGYV 119
           RKAIGAVKDQTSI IAKVAG  APELEVLVVKATSHDEDP DDRYI EIVN C++S+GYV
Sbjct: 7   RKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCSHSNGYV 66

Query: 120 SACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFRD 179
           SACV+TISKRL+KTRDWIVALKALMVVHRVLIDG+PSFGEEIVYATR+G+RVLNLSGFRD
Sbjct: 67  SACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLNLSGFRD 126

Query: 180 EAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGD-NDGSFNHRDEADYGRQRRGQSPW 239
           EAHSNSWDHS FVR YALYLD M+E+LV EK+VRG  +DG   + DE+DYGRQRR QSPW
Sbjct: 127 EAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDG---NSDESDYGRQRRRQSPW 186

Query: 240 RSKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEALG 299
           RS RPERV++E ++LLRMLDRVLACRP+GMAKSSRLVIA + L  +ESFGL +EICEALG
Sbjct: 187 RSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEICEALG 246

Query: 300 LLLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNLL 359
           +LLDHFKEMEYGDCLRAFD   NAAKKID+L+EFYAWCKD+GIARSSEYPEVQRIT NLL
Sbjct: 247 VLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRITHNLL 306

Query: 360 VTLGGFLKEMSRRPKDNDERRREEKLKPLPSEEPEYDMNEIKALPPPEKCDPVLHSV-SQ 419
            TLG FLKEM RRPKD +ERRREEKLK  P+++PE DMNE+KALPPP+  DP LHSV  Q
Sbjct: 307 GTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSLHSVLPQ 366

Query: 420 TRSLKEDLVNLREDGISADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTP 479
           TR  KEDLVNLRED +SADEQGNKLAL LFSGPPITN  GSWETFSSNQ+PEVSSAW+TP
Sbjct: 367 TRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSAWKTP 426

Query: 480 AAEAGKPDWELTLVETSSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVNAEQFGGSSSS 539
           AAEAGK DWEL LVET+SNLSKQKADMGGGFDPLLLN MYDQGTIR+HVNAEQFGGSSSS
Sbjct: 427 AAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQFGGSSSS 486

Query: 540 VAVALQGQGKSVAQVLALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQ 599
           VA+ +QG+    AQVLALPAPDGTV PVGHRDPFAASL VPPPSYVQIAEMEKKQQLL+Q
Sbjct: 487 VALQVQGK-TXAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQLLVQ 546

Query: 600 EQLLWQHYGRDGMHGQVDLAKLAGGSAYVGLNPQPIMPHGSAYYYSPF 646
           EQL+WQ YGR+GMHGQV LAK+ GGSA+V +    IMPH SAYYY+PF
Sbjct: 547 EQLVWQQYGREGMHGQVGLAKIGGGSAHVNV---AIMPHASAYYYAPF 587

BLAST of Spg022497 vs. ExPASy TrEMBL
Match: A0A6J1I3H3 (probable clathrin assembly protein At4g32285 OS=Cucurbita maxima OX=3661 GN=LOC111470668 PE=4 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 7.5e-262
Identity = 472/586 (80.55%), Postives = 509/586 (86.86%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCANSSGYV 119
           RKA+GAVKDQTSI +AKVAG  A ELEVLVVKATSHDEDPAD RYIREIVN+C+ S+GYV
Sbjct: 7   RKALGAVKDQTSISMAKVAGNTASELEVLVVKATSHDEDPADHRYIREIVNWCSTSNGYV 66

Query: 120 SACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFRD 179
           SACV+TISKRLSKTRDWIVALKAL+VVHRVLIDGN SFGEEIVYATRKG+RVL+LS FRD
Sbjct: 67  SACVITISKRLSKTRDWIVALKALVVVHRVLIDGNSSFGEEIVYATRKGIRVLDLSSFRD 126

Query: 180 EAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGDNDGSFNHRDEADYGRQRRGQSPWR 239
           EAHSNSWDHSSFVR YALYLDEM ENLV EKR+RG +D  F+ RDE D  R    QSPWR
Sbjct: 127 EAHSNSWDHSSFVRSYALYLDEMAENLVLEKRMRGSDDTYFDRRDEFDNVR----QSPWR 186

Query: 240 SKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEALGL 299
           S RPE VV+EL++LLR+LDR+LACRP GMAKSSRLVI+ LDLV  +SFGL VEIC+ALG 
Sbjct: 187 SMRPESVVDELSQLLRILDRLLACRPTGMAKSSRLVISALDLVVNDSFGLYVEICKALGY 246

Query: 300 LLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNLLV 359
           LLD FKEMEYGDCLRAFD CANAAKKIDEL EFY WCKDVGIARSSE+PE QRIT NLLV
Sbjct: 247 LLDRFKEMEYGDCLRAFDVCANAAKKIDELDEFYTWCKDVGIARSSEFPEAQRITSNLLV 306

Query: 360 TLGGFLKEMSRRPKDNDERRREEKLKPLPSEEPEYDMNEIKALPPPEKCDPVLHSVSQTR 419
           TLGGFLKEMSRRPKD +ERRREEK+K LP+EEPE  MNEIKALPP E C P L    Q  
Sbjct: 307 TLGGFLKEMSRRPKDYNERRREEKVKSLPTEEPECYMNEIKALPPLENCSPTL----QPP 366

Query: 420 SLKEDLVNLREDGISADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTPAA 479
           S  EDLVNLREDG+SADEQGNKLA  LFSGPP  N  GSWETFSS+++PEVSSAWQTPAA
Sbjct: 367 SRPEDLVNLREDGVSADEQGNKLAWALFSGPP-ANPNGSWETFSSDKQPEVSSAWQTPAA 426

Query: 480 EAGKPDWELTLVETSSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVNAEQFGGSSSSVA 539
           EAGKP+WEL LVET+SNLSKQKADMGGGFDPLLLNGMYD GTIRQH N EQFGGSSSS  
Sbjct: 427 EAGKPEWELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRQHANGEQFGGSSSS-- 486

Query: 540 VALQGQGKSVAQVLALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQEQ 599
           VAL+GQGK+ AQVLALPAPDGTV+PVG +DPFA SL VPPPSYVQIAEMEKKQQLLMQEQ
Sbjct: 487 VALKGQGKTAAQVLALPAPDGTVKPVGVQDPFAVSLTVPPPSYVQIAEMEKKQQLLMQEQ 546

Query: 600 LLWQHYGRDGMHGQVDLAKLAGGSAYVGLNPQPIMPHGSAYYYSPF 646
           LLWQ YG DGMHGQV L KLAGGS Y+G NPQPI P  SAY+Y+PF
Sbjct: 547 LLWQQYGIDGMHGQVGLVKLAGGSVYIGPNPQPIAPQSSAYHYAPF 581

BLAST of Spg022497 vs. ExPASy TrEMBL
Match: A0A5D3C4X9 (Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00870 PE=4 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 2.6e-238
Identity = 435/567 (76.72%), Postives = 480/567 (84.66%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCAN-SSGY 119
           RKAIGA+KDQT+I IAKVAG  A EL+VLVVKATSHDEDPADDRYIREIVN C+N S+GY
Sbjct: 7   RKAIGAMKDQTTISIAKVAGNMARELDVLVVKATSHDEDPADDRYIREIVNLCSNYSNGY 66

Query: 120 VSACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFR 179
           V+ACV+TISKRLSKTRDWIVALKAL+VVHRVLIDG+PSFGEEIVYATRKG RVL+LSGFR
Sbjct: 67  VNACVVTISKRLSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGTRVLDLSGFR 126

Query: 180 DEAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGDNDGSFNHRDEADYGRQRRGQSPW 239
           DEAHSNSWDHSSF+RFYALYLDE++E LV EK+V+G         DE+ Y  +R  QS W
Sbjct: 127 DEAHSNSWDHSSFLRFYALYLDELIETLVLEKKVKGG--------DESYYRGKR--QSSW 186

Query: 240 RSKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEALG 299
           RS RPERVVEELN+L+RMLDRVLACRP GMAKSSRLVI  L+LV +ESF L +EIC+ALG
Sbjct: 187 RSIRPERVVEELNQLIRMLDRVLACRPNGMAKSSRLVITALNLVIKESFRLYIEICDALG 246

Query: 300 LLLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNLL 359
           +LLD FKEMEY DCLR FD CANA K IDELVEFY WCKDVGIARSSEYP V+ IT NLL
Sbjct: 247 VLLDQFKEMEYADCLRVFDVCANAVKNIDELVEFYCWCKDVGIARSSEYPGVRMITNNLL 306

Query: 360 VTLGGFLKEMSRRPKDNDERRREEKLKPLPSEEPEYDMNEIKALPPPEKCDPVLHSVSQT 419
           +TLGGFLKEM+RRPKD +E R +E +KP+  +E  YDMNEIKALPPPE C PV  SV Q 
Sbjct: 307 ITLGGFLKEMTRRPKDYNEMRTKE-MKPVSVKESRYDMNEIKALPPPENCAPVSQSVLQ- 366

Query: 420 RSLKEDLVNLREDGISADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTPA 479
            SLKEDLVNLRED +SADEQG KLA+  FSGPP TN  GSWETFS N EPEVSS WQT A
Sbjct: 367 -SLKEDLVNLREDEVSADEQGEKLAMAFFSGPPTTNPNGSWETFSLNHEPEVSSVWQTRA 426

Query: 480 AEAGKPDWELTLVETSSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVNAEQFGGSSSSV 539
           AE GKPDWEL LVET+S+LSKQ+ DMGG FDP+LLNGMYDQ  +R+HVN EQ GGSSSSV
Sbjct: 427 AEFGKPDWELALVETASHLSKQRPDMGGCFDPMLLNGMYDQEAVRRHVNVEQLGGSSSSV 486

Query: 540 AVALQGQGKSVAQVLALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQE 599
           AVALQGQG + +QVLALPAPDGTV+ VG RDPFAASL VPPPSYVQIAEMEKKQQLL QE
Sbjct: 487 AVALQGQGSAGSQVLALPAPDGTVELVGQRDPFAASLMVPPPSYVQIAEMEKKQQLLAQE 546

Query: 600 QLLWQHYGRDGMHGQVDLAKLAGGSAY 626
           QLLW+ YGRDGM GQV L K+  GSAY
Sbjct: 547 QLLWKQYGRDGMQGQVGLEKVGNGSAY 560

BLAST of Spg022497 vs. ExPASy TrEMBL
Match: A0A1S3B992 (putative clathrin assembly protein At2g25430 OS=Cucumis melo OX=3656 GN=LOC103487428 PE=4 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 2.6e-238
Identity = 435/567 (76.72%), Postives = 480/567 (84.66%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCAN-SSGY 119
           RKAIGA+KDQT+I IAKVAG  A EL+VLVVKATSHDEDPADDRYIREIVN C+N S+GY
Sbjct: 7   RKAIGAMKDQTTISIAKVAGNMARELDVLVVKATSHDEDPADDRYIREIVNLCSNYSNGY 66

Query: 120 VSACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFR 179
           V+ACV+TISKRLSKTRDWIVALKAL+VVHRVLIDG+PSFGEEIVYATRKG RVL+LSGFR
Sbjct: 67  VNACVVTISKRLSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGTRVLDLSGFR 126

Query: 180 DEAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGDNDGSFNHRDEADYGRQRRGQSPW 239
           DEAHSNSWDHSSF+RFYALYLDE++E LV EK+V+G         DE+ Y  +R  QS W
Sbjct: 127 DEAHSNSWDHSSFLRFYALYLDELIETLVLEKKVKGG--------DESYYRGKR--QSSW 186

Query: 240 RSKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEALG 299
           RS RPERVVEELN+L+RMLDRVLACRP GMAKSSRLVI  L+LV +ESF L +EIC+ALG
Sbjct: 187 RSIRPERVVEELNQLIRMLDRVLACRPNGMAKSSRLVITALNLVIKESFRLYIEICDALG 246

Query: 300 LLLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNLL 359
           +LLD FKEMEY DCLR FD CANA K IDELVEFY WCKDVGIARSSEYP V+ IT NLL
Sbjct: 247 VLLDQFKEMEYADCLRVFDVCANAVKNIDELVEFYCWCKDVGIARSSEYPGVRMITNNLL 306

Query: 360 VTLGGFLKEMSRRPKDNDERRREEKLKPLPSEEPEYDMNEIKALPPPEKCDPVLHSVSQT 419
           +TLGGFLKEM+RRPKD +E R +E +KP+  +E  YDMNEIKALPPPE C PV  SV Q 
Sbjct: 307 ITLGGFLKEMTRRPKDYNEMRTKE-MKPVSVKESRYDMNEIKALPPPENCAPVSQSVLQ- 366

Query: 420 RSLKEDLVNLREDGISADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTPA 479
            SLKEDLVNLRED +SADEQG KLA+  FSGPP TN  GSWETFS N EPEVSS WQT A
Sbjct: 367 -SLKEDLVNLREDEVSADEQGEKLAMAFFSGPPTTNPNGSWETFSLNHEPEVSSVWQTRA 426

Query: 480 AEAGKPDWELTLVETSSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVNAEQFGGSSSSV 539
           AE GKPDWEL LVET+S+LSKQ+ DMGG FDP+LLNGMYDQ  +R+HVN EQ GGSSSSV
Sbjct: 427 AEFGKPDWELALVETASHLSKQRPDMGGCFDPMLLNGMYDQEAVRRHVNVEQLGGSSSSV 486

Query: 540 AVALQGQGKSVAQVLALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQE 599
           AVALQGQG + +QVLALPAPDGTV+ VG RDPFAASL VPPPSYVQIAEMEKKQQLL QE
Sbjct: 487 AVALQGQGSAGSQVLALPAPDGTVELVGQRDPFAASLMVPPPSYVQIAEMEKKQQLLAQE 546

Query: 600 QLLWQHYGRDGMHGQVDLAKLAGGSAY 626
           QLLW+ YGRDGM GQV L K+  GSAY
Sbjct: 547 QLLWKQYGRDGMQGQVGLEKVGNGSAY 560

BLAST of Spg022497 vs. TAIR 10
Match: AT4G32285.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 621.3 bits (1601), Expect = 8.6e-178
Identity = 340/634 (53.63%), Postives = 424/634 (66.88%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCANSSGYV 119
           RKAIG VKDQTSIGIAKVA   AP+LEV +VKATSHD+D + D+YIREI++  + S GYV
Sbjct: 6   RKAIGVVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRGYV 65

Query: 120 SACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFRD 179
            ACV ++S+RL KTRDWIVALKALM+VHR+L +G+P F EEI+YATR+G R+LN+S FRD
Sbjct: 66  HACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDFRD 125

Query: 180 EAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGDNDGSFNHRDEADYG---------- 239
           EAHS+SWDHS+FVR YA YLD+ +E  +FE+R R     S +H+   D G          
Sbjct: 126 EAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDDFRS 185

Query: 240 --------------------------------RQRRGQSPWRSKRPERVVEELNRLLRML 299
                                            +++  +P R   PER+  ++  L R+L
Sbjct: 186 PPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFGKMGHLQRLL 245

Query: 300 DRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEALGLLLDHFKEMEYGDCLRAFD 359
           DR L+CRP G+AK+SR+++  +  V +ESF L  +ICE L +LLD F +MEY DC++AFD
Sbjct: 246 DRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTDCVKAFD 305

Query: 360 ACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNLLVTLGGFLKEMSRRPKDNDE 419
           A A+AAK+IDEL+ FY WCKD G+ARSSEYPEVQRIT  LL TL  F+++ ++R K  + 
Sbjct: 306 AYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKRAKSPER 365

Query: 420 RRREEKLKPLPSEEPEYDMNEIKALPPPEK----CDPVLHSVSQTRSLKEDLVNLREDGI 479
           +  E    P P  E   DMNEIKALPPPE       P      Q   + +DLVNLRED +
Sbjct: 366 KEIEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPAPEPKPQQPQVTDDLVNLREDDV 425

Query: 480 SADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTPAAEAGKPDWELTLVET 539
           S D+QGNK AL LF+GPP  N  G WE FSS+    V+SAWQ PAAE GK DWEL LVET
Sbjct: 426 SGDDQGNKFALALFAGPPANN--GKWEAFSSDN--NVTSAWQNPAAELGKADWELALVET 485

Query: 540 SSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVN-AEQFGGSSSSVAVALQGQGKSVAQV 599
           +SNL  QKA MGGG DPLLLNGMYDQG +RQHV+ +E  GGSSSSVA+ L G+  S   +
Sbjct: 486 ASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELTGGSSSSVALPLPGKVNS--HI 545

Query: 600 LALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQEQLLWQHYGRDGMHG 644
           LALPAPDGTVQ V ++DPFAASL +PPPSYVQ+AEM+KKQ LL QEQ LWQ Y ++GM G
Sbjct: 546 LALPAPDGTVQKV-NQDPFAASLTIPPPSYVQMAEMDKKQYLLTQEQQLWQQYQQEGMRG 605

BLAST of Spg022497 vs. TAIR 10
Match: AT4G32285.2 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 621.3 bits (1601), Expect = 8.6e-178
Identity = 340/634 (53.63%), Postives = 424/634 (66.88%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCANSSGYV 119
           RKAIG VKDQTSIGIAKVA   AP+LEV +VKATSHD+D + D+YIREI++  + S GYV
Sbjct: 6   RKAIGVVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRGYV 65

Query: 120 SACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFRD 179
            ACV ++S+RL KTRDWIVALKALM+VHR+L +G+P F EEI+YATR+G R+LN+S FRD
Sbjct: 66  HACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDFRD 125

Query: 180 EAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGDNDGSFNHRDEADYG---------- 239
           EAHS+SWDHS+FVR YA YLD+ +E  +FE+R R     S +H+   D G          
Sbjct: 126 EAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDDFRS 185

Query: 240 --------------------------------RQRRGQSPWRSKRPERVVEELNRLLRML 299
                                            +++  +P R   PER+  ++  L R+L
Sbjct: 186 PPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFGKMGHLQRLL 245

Query: 300 DRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEALGLLLDHFKEMEYGDCLRAFD 359
           DR L+CRP G+AK+SR+++  +  V +ESF L  +ICE L +LLD F +MEY DC++AFD
Sbjct: 246 DRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTDCVKAFD 305

Query: 360 ACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNLLVTLGGFLKEMSRRPKDNDE 419
           A A+AAK+IDEL+ FY WCKD G+ARSSEYPEVQRIT  LL TL  F+++ ++R K  + 
Sbjct: 306 AYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKRAKSPER 365

Query: 420 RRREEKLKPLPSEEPEYDMNEIKALPPPEK----CDPVLHSVSQTRSLKEDLVNLREDGI 479
           +  E    P P  E   DMNEIKALPPPE       P      Q   + +DLVNLRED +
Sbjct: 366 KEIEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPAPEPKPQQPQVTDDLVNLREDDV 425

Query: 480 SADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTPAAEAGKPDWELTLVET 539
           S D+QGNK AL LF+GPP  N  G WE FSS+    V+SAWQ PAAE GK DWEL LVET
Sbjct: 426 SGDDQGNKFALALFAGPPANN--GKWEAFSSDN--NVTSAWQNPAAELGKADWELALVET 485

Query: 540 SSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVN-AEQFGGSSSSVAVALQGQGKSVAQV 599
           +SNL  QKA MGGG DPLLLNGMYDQG +RQHV+ +E  GGSSSSVA+ L G+  S   +
Sbjct: 486 ASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELTGGSSSSVALPLPGKVNS--HI 545

Query: 600 LALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQEQLLWQHYGRDGMHG 644
           LALPAPDGTVQ V ++DPFAASL +PPPSYVQ+AEM+KKQ LL QEQ LWQ Y ++GM G
Sbjct: 546 LALPAPDGTVQKV-NQDPFAASLTIPPPSYVQMAEMDKKQYLLTQEQQLWQQYQQEGMRG 605

BLAST of Spg022497 vs. TAIR 10
Match: AT2G25430.1 (epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related )

HSP 1 Score: 616.3 bits (1588), Expect = 2.8e-176
Identity = 353/654 (53.98%), Postives = 440/654 (67.28%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAGTAAPELEVLVVKATSHDEDPADDRYIREIVNFCANSSGYV 119
           RKAIGAVKDQTSIGIAKVA   AP+LEV +VKATSHD+DPA ++YIREI+N  + S GY+
Sbjct: 6   RKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTSLSRGYI 65

Query: 120 SACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGFRD 179
            ACV ++S+RLSKTRDW+VALKALM+VHR+L +G+P F EEI+Y+TR+G R+LN+S FRD
Sbjct: 66  LACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRMLNMSDFRD 125

Query: 180 EAHSNSWDHSSFVRFYALYLDEMVENLVFEKR---------------------------- 239
           EAHS+SWDHS+FVR YA YLD+ +E  +FE++                            
Sbjct: 126 EAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDDRYGRGRDDF 185

Query: 240 -------------------VRGDNDG-------SFNHRDEADYG--------RQRRGQSP 299
                               RGDN+G       S ++ D  + G         +++  +P
Sbjct: 186 RSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGGGRDEKKVVTP 245

Query: 300 WRSKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVARESFGLCVEICEAL 359
            R   PER+  ++  L R+LDR L+ RP G+AK+SR+++  L  V RESF L  +ICE L
Sbjct: 246 LREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFKLYADICEVL 305

Query: 360 GLLLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARSSEYPEVQRITQNL 419
            +LLD F +MEY DC++AFDA A+AAK+IDEL+ FY WCK+ G+ARSSEYPEVQRIT  L
Sbjct: 306 AVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYPEVQRITSKL 365

Query: 420 LVTLGGFLKEMSRRPKDNDERRREEKLKPLPSEEPEYDMNEIKALPPPEKCDPVLHSVSQ 479
           L TL  F+++ ++R K  + +  E     +  EEPE DMNEIKALPPPE   P      +
Sbjct: 366 LETLEEFVRDRAKRGKSPERKEIEAPPPVVEEEEPEPDMNEIKALPPPENYTPPPPPEPE 425

Query: 480 TRSLK----EDLVNLREDGISADEQGNKLALDLFSGPPITNSYGSWETFSSNQEPEVSSA 539
            +  K    EDLVNLRED ++AD+QGNK AL LF+GPP  N  G WE FSSN    V+SA
Sbjct: 426 PQPEKPQFTEDLVNLREDEVTADDQGNKFALALFAGPPGNN--GKWEAFSSN---GVTSA 485

Query: 540 WQTPAAEAGKPDWELTLVETSSNLSKQKADMGGGFDPLLLNGMYDQGTIRQHVNAEQF-G 599
           WQ PAAE GK DWEL LVET+SNL KQ A +GGGFD LLLNGMYDQG +RQHV+  Q  G
Sbjct: 486 WQNPAAEPGKADWELALVETTSNLEKQTAALGGGFDNLLLNGMYDQGMVRQHVSTSQLTG 545

Query: 600 GSSSSVAVALQGQGKSVAQVLALPAPDGTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQ 644
           GS+SSVA+ L   GK+  QVLALPAPDGTV+ V ++DPFAASL +PPPSYVQ+AEMEKKQ
Sbjct: 546 GSASSVALPL--PGKTNNQVLALPAPDGTVEKV-NQDPFAASLTIPPPSYVQMAEMEKKQ 605

BLAST of Spg022497 vs. TAIR 10
Match: AT1G03050.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 413.7 bits (1062), Expect = 2.7e-115
Identity = 242/602 (40.20%), Postives = 364/602 (60.47%), Query Frame = 0

Query: 59  YRKAIGAVKDQTSIGIAKVAGTAA--PELEVLVVKATSHDEDPADDRYIREIVNFCANSS 118
           +++AIGAVKDQTS+G+AKV G +A   EL+V +VKAT H+E PA+++YIREI++  + S 
Sbjct: 6   FKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSLTSYSR 65

Query: 119 GYVSACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSG 178
            Y++ACV T+S+RL+KT+ W VALK L+++ R+L +G+ ++ +EI +ATR+G R+LN+S 
Sbjct: 66  SYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRLLNMSD 125

Query: 179 FRDEAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVRGDNDGSFNHRDEADYGRQRRG-- 238
           FRD + SNSWD+S+FVR YALYLDE ++   F  + R    G +    EAD   Q +   
Sbjct: 126 FRDVSRSNSWDYSAFVRTYALYLDERLD---FRMQARHGKRGVYCVGGEADEEEQDQAAA 185

Query: 239 ---------QSPWRSKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVARE 298
                      P    + E++   +  L ++LDR LACRP G A+++R+VI  L  + +E
Sbjct: 186 DLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPIVKE 245

Query: 299 SFGLCVEICEALGLLLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARSS 358
           SF +  ++ E +G+L++ F E++  D ++ +D     +K+ +EL +FY+WCK++GIARSS
Sbjct: 246 SFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIARSS 305

Query: 359 EYPEVQRITQNLLVTLGGFLKE-------------MSRRPKDNDERRREEKLKPLPSEEP 418
           EYPE+++ITQ  L  +  F+++              S   +D+DE R EE        E 
Sbjct: 306 EYPEIEKITQKKLDLMDEFIRDKSALEHTKQSKSVKSEADEDDDEARTEE------VNEE 365

Query: 419 EYDMNEIKAL--PPPEKCDPVLHS--------VSQTRSLKEDLVNL-REDGISADEQGNK 478
           + DMN IKAL  PPP++ D V           + + +    DL++L   +G  A + G+ 
Sbjct: 366 QEDMNAIKALPEPPPKEEDDVKPEEEAKEEVIIEKKQEEMGDLLDLGNTNGGEAGQAGDS 425

Query: 479 LALDLFSGPPITNSYGSWETFSSNQEPEVSSAWQTPAAEAGKPDWELTLVETSSNLSKQK 538
           LAL LF GP           ++S    E    W+  A +    DWE  LV+T++NLS QK
Sbjct: 426 LALALFDGP-----------YASGSGSESGPGWE--AFKDDSADWETALVQTATNLSGQK 485

Query: 539 ADMGGGFDPLLLNGMYDQGTIRQHV-NAEQFGGSSSSVAVALQGQGKSVAQVLALPAPD- 598
           +++GGGFD LLLNGMY  G +   V  +  +G S S+ ++A    G+  A +LALPAP  
Sbjct: 486 SELGGGFDMLLLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATMLALPAPST 545

Query: 599 -----GTVQPVGHRDPFAASLAVPPPSYVQIAEMEKKQQLLMQEQLLWQHYGRDGMHGQV 617
                G +      DPFAASL V PP+YVQ+ +MEKKQ++LM+EQ++W  Y RDG  G +
Sbjct: 546 ANGNAGNINSPVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSRDGRQGHM 585

BLAST of Spg022497 vs. TAIR 10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 407.1 bits (1045), Expect = 2.6e-113
Identity = 239/602 (39.70%), Postives = 359/602 (59.63%), Query Frame = 0

Query: 60  RKAIGAVKDQTSIGIAKVAG--TAAPELEVLVVKATSHDEDPADDRYIREIVNFCANSSG 119
           ++AIGAVKDQTS+G+AKV G  ++  ELE+ VVKAT HD+ PA+D+YIREI+   + S  
Sbjct: 7   KRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCLTSYSRN 66

Query: 120 YVSACVLTISKRLSKTRDWIVALKALMVVHRVLIDGNPSFGEEIVYATRKGVRVLNLSGF 179
           YVSACV T+S+RL+KT++W VALK L+++ R+L DG+ ++ +EI +ATR+G R+LN+S F
Sbjct: 67  YVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRLLNMSDF 126

Query: 180 RDEAHSNSWDHSSFVRFYALYLDEMVENLVFEKRVR----------GDNDGSFNHRDEAD 239
           RD + S+SWD+S+FVR YALYLDE ++  +  +R +          GD+    +HR  ++
Sbjct: 127 RDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEEDDHRGTSN 186

Query: 240 YGRQRR---GQSPWRSKRPERVVEELNRLLRMLDRVLACRPIGMAKSSRLVIAGLDLVAR 299
             R +       P    + E++   +  L ++LDR LACRP G AK++R+VI  +  + +
Sbjct: 187 DIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIVAMYPIVK 246

Query: 300 ESFGLCVEICEALGLLLDHFKEMEYGDCLRAFDACANAAKKIDELVEFYAWCKDVGIARS 359
           ESF L   I E +G+L++ F E++  D ++ ++     +K+ DEL  FY WCK++ +ARS
Sbjct: 247 ESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCKNMAVARS 306

Query: 360 SEYPEVQRITQNLLVTLGGFLKEMS-------RRPKDNDERRREEKLKPLPSEEPEYDMN 419
           SEYPE+++ITQ  L  +  F+++ S       +       +  EE+ K    +E + D+N
Sbjct: 307 SEYPELEKITQKKLDLMDEFIRDKSALAAQTTKSSSKRSNKSEEEESKTEYIQENQEDLN 366

Query: 420 EIKALPPPEKCDPV-----------LHSVSQTRSLKEDLVNLRED-GISADEQGNKLALD 479
            IKALP PE+ +             +  V   +  + DL++L ++ G++A   G+ LAL 
Sbjct: 367 SIKALPAPEQKEEEEEEEKMETKKDVEEVVSRQDQEGDLLDLTDEAGVTAGTVGDSLALA 426

Query: 480 LFSGPPITNSYG--SWETFSSNQEPEVSSAWQTPAAEAGKPDWELTLVETSSNLSKQKAD 539
           LF G   T S     WE F+ N                   DWE  LV +++ LS QK++
Sbjct: 427 LFDGVVGTESASGPGWEAFNDN-----------------SADWETDLVRSATRLSGQKSE 486

Query: 540 MGGGFDPLLLNGMYDQGTIRQHV-NAEQFGGSSSSVAVALQGQGKSVAQVLALPAPDGTV 599
           +GGGFD LLL+GMY  G +   V  +  +G S S+ +VA    G   A +LALPAP  T 
Sbjct: 487 LGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSASSVAFGSAGSPAASMLALPAPPPTA 546

Query: 600 QPVGHR------DPFAASLAVPPPSYVQIAEMEKKQQLLMQEQLLWQHYGRDGMHGQVDL 619
              G+R      DPFAASL V PP+YVQ+ +MEKKQ+LLM+EQ++W  Y R G  G ++ 
Sbjct: 547 N--GNRNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSGRQGHMNF 589

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022934011.11.9e-26781.74probable clathrin assembly protein At4g32285 [Cucurbita moschata][more]
XP_023529315.15.5e-26781.74putative clathrin assembly protein At2g25430 [Cucurbita pepo subsp. pepo][more]
KAG6587890.11.2e-26681.57putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022145098.15.1e-26580.95probable clathrin assembly protein At4g32285 [Momordica charantia][more]
XP_038879765.19.7e-26479.73putative clathrin assembly protein At2g25430 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8S9J81.2e-17653.63Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8LF203.9e-17553.98Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9SA653.9e-11440.20Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8GX473.6e-11239.70Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9ZVN65.3e-6341.16Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1F0M39.1e-26881.74probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1CV162.5e-26580.95probable clathrin assembly protein At4g32285 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1I3H37.5e-26280.55probable clathrin assembly protein At4g32285 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A5D3C4X92.6e-23876.72Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3B9922.6e-23876.72putative clathrin assembly protein At2g25430 OS=Cucumis melo OX=3656 GN=LOC10348... [more]
Match NameE-valueIdentityDescription
AT4G32285.18.6e-17853.63ENTH/ANTH/VHS superfamily protein [more]
AT4G32285.28.6e-17853.63ENTH/ANTH/VHS superfamily protein [more]
AT2G25430.12.8e-17653.98epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly p... [more]
AT1G03050.12.7e-11540.20ENTH/ANTH/VHS superfamily protein [more]
AT4G02650.12.6e-11339.70ENTH/ANTH/VHS superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013809ENTH domainSMARTSM00273enth_2coord: 83..213
e-value: 1.2E-33
score: 127.8
IPR013809ENTH domainPROSITEPS50942ENTHcoord: 77..213
score: 30.799936
IPR011417AP180 N-terminal homology (ANTH) domainPFAMPF07651ANTHcoord: 84..366
e-value: 3.9E-68
score: 229.4
IPR014712ANTH domain superfamilyGENE3D1.20.58.150ANTH domaincoord: 230..384
e-value: 1.2E-28
score: 101.5
IPR008942ENTH/VHSGENE3D1.25.40.90coord: 58..208
e-value: 5.6E-38
score: 131.9
IPR008942ENTH/VHSSUPERFAMILY48464ENTH/VHS domaincoord: 83..204
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 370..394
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 219..241
NoneNo IPR availablePANTHERPTHR22951CLATHRIN ASSEMBLY PROTEINcoord: 59..627
NoneNo IPR availablePANTHERPTHR22951:SF62ASSEMBLY PLANT-LIKE PROTEIN, PUTATIVE-RELATEDcoord: 59..627
NoneNo IPR availableCDDcd16987ANTH_N_AP180_plantcoord: 85..204
e-value: 1.69953E-56
score: 185.135
NoneNo IPR availableSUPERFAMILY89009GAT-like domaincoord: 234..361

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg022497.1Spg022497.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048268 clathrin coat assembly
biological_process GO:0072583 clathrin-dependent endocytosis
biological_process GO:0006900 vesicle budding from membrane
cellular_component GO:0005905 clathrin-coated pit
cellular_component GO:0030136 clathrin-coated vesicle
cellular_component GO:0005794 Golgi apparatus
molecular_function GO:0005545 1-phosphatidylinositol binding
molecular_function GO:0032050 clathrin heavy chain binding
molecular_function GO:0005546 phosphatidylinositol-4,5-bisphosphate binding
molecular_function GO:0000149 SNARE binding
molecular_function GO:0030276 clathrin binding
molecular_function GO:0005543 phospholipid binding