Spg022467 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022467
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Calcium-binding EF-hand family protein
Location	scaffold2: 3257172 .. 3257732 (+)
RNA-Seq Expression	Spg022467
Synteny	Spg022467

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCACCGACCCCAACCCGATTCCGGCCTCGAGCTCCGACGCCGACCAGGTCTCCAAGTCCAAACCCTCCGTCTTCCTCCAGGACAACGTCGAACTCCGCAAGGTTTTCGAACGCTTCGATGCGAATGGCGACGGCAAGATCTCCATCTCCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTCCGCTCCGTCATGGAGGATCTCGACGCCGATAAGGACGGTTTCATCAACATCGACGAGTTCGACGCCTTCTGCAAGGAACCGATGGCCTCCGACGAGGCCGGAGTGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCACAACGGCCTTATCTCGCACTCCGAGCTCCATCTCGTCCTCAATCGCCTCGGAATCAGTTGCTCCAAGGAGGACTGTCAACGGATGATCCACTCCGTCGATTCCGATGGCGACGGGAATGTCAATTTCGAAGAGTTTAGGAAGATGATGACCACTAATTCCAACAACAAGGCGCCGAATCAGAATGGAACTGCCGCCGCAGCGCCCTAG

mRNA sequence

ATGGCCACCGACCCCAACCCGATTCCGGCCTCGAGCTCCGACGCCGACCAGGTCTCCAAGTCCAAACCCTCCGTCTTCCTCCAGGACAACGTCGAACTCCGCAAGGTTTTCGAACGCTTCGATGCGAATGGCGACGGCAAGATCTCCATCTCCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTCCGCTCCGTCATGGAGGATCTCGACGCCGATAAGGACGGTTTCATCAACATCGACGAGTTCGACGCCTTCTGCAAGGAACCGATGGCCTCCGACGAGGCCGGAGTGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCACAACGGCCTTATCTCGCACTCCGAGCTCCATCTCGTCCTCAATCGCCTCGGAATCAGTTGCTCCAAGGAGGACTGTCAACGGATGATCCACTCCGTCGATTCCGATGGCGACGGGAATGTCAATTTCGAAGAGTTTAGGAAGATGATGACCACTAATTCCAACAACAAGGCGCCGAATCAGAATGGAACTGCCGCCGCAGCGCCCTAG

Coding sequence (CDS)

ATGGCCACCGACCCCAACCCGATTCCGGCCTCGAGCTCCGACGCCGACCAGGTCTCCAAGTCCAAACCCTCCGTCTTCCTCCAGGACAACGTCGAACTCCGCAAGGTTTTCGAACGCTTCGATGCGAATGGCGACGGCAAGATCTCCATCTCCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTCCGCTCCGTCATGGAGGATCTCGACGCCGATAAGGACGGTTTCATCAACATCGACGAGTTCGACGCCTTCTGCAAGGAACCGATGGCCTCCGACGAGGCCGGAGTGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCACAACGGCCTTATCTCGCACTCCGAGCTCCATCTCGTCCTCAATCGCCTCGGAATCAGTTGCTCCAAGGAGGACTGTCAACGGATGATCCACTCCGTCGATTCCGATGGCGACGGGAATGTCAATTTCGAAGAGTTTAGGAAGATGATGACCACTAATTCCAACAACAAGGCGCCGAATCAGAATGGAACTGCCGCCGCAGCGCCCTAG

Protein sequence

MATDPNPIPASSSDADQVSKSKPSVFLQDNVELRKVFERFDANGDGKISISELDSVLKSLGSAIPYEELRSVMEDLDADKDGFINIDEFDAFCKEPMASDEAGVSELRDAFDLYDQDHNGLISHSELHLVLNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTTNSNNKAPNQNGTAAAAP

Homology

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BLAST of Spg022467 vs. NCBI nr
Match: XP_038878467.1 (probable calcium-binding protein CML27 [Benincasa hispida])

HSP 1 Score: 343.2 bits (879), Expect = 1.4e-90
Identity = 174/186 (93.55%), Postives = 179/186 (96.24%), Query Frame = 0


Query: 1   MATDPNPIPASSSDADQVSKSKPSVFLQDNVELRKVFERFDANGDGKISISELDSVLKSL 60
           MATDPN IPAS+SDADQVSK KPSVFLQDN ELRKVFERFDANGDGKISISELDSVLKSL
Sbjct: 1   MATDPNQIPASNSDADQVSKPKPSVFLQDNEELRKVFERFDANGDGKISISELDSVLKSL 60

Query: 61  GSAIPYEELRSVMEDLDADKDGFINIDEFDAFCKEPMASDEAGVSELRDAFDLYDQDHNG 120
           GS+IPYEELRSVMEDLD+DKDG+INIDEF AFCKEPMASDEAG SELRDAFDLYDQDHNG
Sbjct: 61  GSSIPYEELRSVMEDLDSDKDGYINIDEFAAFCKEPMASDEAGASELRDAFDLYDQDHNG 120

Query: 121 LISHSELHLVLNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTTNSNNKAPNQNG 180
           LISHSELHLVLNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMT NSN+KA NQNG
Sbjct: 121 LISHSELHLVLNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTANSNSKAANQNG 180

Query: 181 TAAAAP 187
           TAAAAP
Sbjct: 181 TAAAAP 186

[Close]

BLAST of Spg022467 vs. NCBI nr
Match: XP_023529417.1 (probable calcium-binding protein CML27 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 317.0 bits (811), Expect = 1.1e-82
Identity = 158/186 (84.95%), Postives = 171/186 (91.94%), Query Frame = 0


Query: 1   MATDPNPIPASSSDADQVSKSKPSVFLQDNVELRKVFERFDANGDGKISISELDSVLKSL 60
           MATD N I AS+SD +QV KSKPS  LQDN ELRKVF+RFDANGDGKIS+SELDSVLKSL
Sbjct: 1   MATDSNQIAASNSDTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60

Query: 61  GSAIPYEELRSVMEDLDADKDGFINIDEFDAFCKEPMASDEAGVSELRDAFDLYDQDHNG 120
           GS++PY+ELR+VMEDLD DKDGFINIDEF  FCKEPMAS+EAG SELRDAFDLYDQDHNG
Sbjct: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG 120

Query: 121 LISHSELHLVLNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTTNSNNKAPNQNG 180
           LIS SELHLVLNRLGI+CSKEDCQRMIHSVDSDGDGNVNFEEFRKMM+ NSN++APNQNG
Sbjct: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNG 180

Query: 181 TAAAAP 187
           TAA AP
Sbjct: 181 TAATAP 186

[Close]

BLAST of Spg022467 vs. NCBI nr
Match: XP_022933917.1 (probable calcium-binding protein CML27 [Cucurbita moschata] >KAG7021647.1 putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 315.8 bits (808), Expect = 2.4e-82
Identity = 157/186 (84.41%), Postives = 171/186 (91.94%), Query Frame = 0


Query: 1   MATDPNPIPASSSDADQVSKSKPSVFLQDNVELRKVFERFDANGDGKISISELDSVLKSL 60
           MATD N I AS++D +QV KSKPS  LQDN ELRKVF+RFDANGDGKIS+SELDSVLKSL
Sbjct: 1   MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60

Query: 61  GSAIPYEELRSVMEDLDADKDGFINIDEFDAFCKEPMASDEAGVSELRDAFDLYDQDHNG 120
           GS++PY+ELR+VMEDLD DKDGFINIDEF  FCKEPMAS+EAG SELRDAFDLYDQDHNG
Sbjct: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG 120

Query: 121 LISHSELHLVLNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTTNSNNKAPNQNG 180
           LIS SELHLVLNRLGI+CSKEDCQRMIHSVDSDGDGNVNFEEFRKMM+ NSN++APNQNG
Sbjct: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNG 180

Query: 181 TAAAAP 187
           TAA AP
Sbjct: 181 TAATAP 186

[Close]

BLAST of Spg022467 vs. NCBI nr
Match: KAG6587683.1 (putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 312.4 bits (799), Expect = 2.7e-81
Identity = 156/186 (83.87%), Postives = 170/186 (91.40%), Query Frame = 0


Query: 1   MATDPNPIPASSSDADQVSKSKPSVFLQDNVELRKVFERFDANGDGKISISELDSVLKSL 60
           MATD N I AS++D +QV KSKPS  LQDN ELRKVF+RFDANGDGKIS+SELDSVLKSL
Sbjct: 1   MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60

Query: 61  GSAIPYEELRSVMEDLDADKDGFINIDEFDAFCKEPMASDEAGVSELRDAFDLYDQDHNG 120
           GS++P +ELR+VMEDLD DKDGFINIDEF  FCKEPMAS+EAG SELRDAFDLYDQDHNG
Sbjct: 61  GSSVPNDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG 120

Query: 121 LISHSELHLVLNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTTNSNNKAPNQNG 180
           LIS SELHLVLNRLGI+CSKEDCQRMIHSVDSDGDGNVNFEEFRKMM+ NSN++APNQNG
Sbjct: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNG 180

Query: 181 TAAAAP 187
           TAA AP
Sbjct: 181 TAATAP 186

[Close]

BLAST of Spg022467 vs. NCBI nr
Match: XP_023003784.1 (probable calcium-binding protein CML27 [Cucurbita maxima])

HSP 1 Score: 310.8 bits (795), Expect = 7.7e-81
Identity = 155/186 (83.33%), Postives = 169/186 (90.86%), Query Frame = 0


Query: 1   MATDPNPIPASSSDADQVSKSKPSVFLQDNVELRKVFERFDANGDGKISISELDSVLKSL 60
           MATD N I AS+SD +QV KSKPS  LQDN ELRKVF+RFDANGDGKIS+SELDSVLKS 
Sbjct: 1   MATDLNQIAASNSDTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSS 60

Query: 61  GSAIPYEELRSVMEDLDADKDGFINIDEFDAFCKEPMASDEAGVSELRDAFDLYDQDHNG 120
           GS++PY+ELR+VMEDLD DKDGFINIDEF  FCKEP+AS+EAG SELRDAFDLYDQDHNG
Sbjct: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPIASNEAGASELRDAFDLYDQDHNG 120

Query: 121 LISHSELHLVLNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTTNSNNKAPNQNG 180
           LIS SELHLVLNRLGI+CSKEDCQRMIHSVDSDGDGNVNFEEFRKMM+ NSN++ PNQNG
Sbjct: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEVPNQNG 180

Query: 181 TAAAAP 187
           TAA AP
Sbjct: 181 TAATAP 186

[Close]

BLAST of Spg022467 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.2e-44
Identity = 93/173 (53.76%), Postives = 126/173 (72.83%), Query Frame = 0


Query: 11  SSSDADQVSKSKPSVFLQDNVELRKVFERFDANGDGKISISELDSVLKSLGSAIPYEELR 70
           +S++ +    +  +V + +  EL+KVF++FD+NGDGKIS+ EL  V K++G++    EL 
Sbjct: 2   ASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELN 61

Query: 71  SVMEDLDADKDGFINIDEFDAFCKEPMASDEAGVSELRDAFDLYDQDHNGLISHSELHLV 130
            V+E++D D+DG+IN+DEF   C+       +  +E+RDAFDLYDQD NGLIS SELH V
Sbjct: 62  RVLEEVDTDRDGYINLDEFSTLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQV 121

Query: 131 LNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTTNSNNKAPNQNGTAA 184
           LNRLG+SCS EDC RMI  VD+DGDGNVNFEEF+KMMT++S     N NG+AA
Sbjct: 122 LNRLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS---LLNSNGSAA 166

[Close]

BLAST of Spg022467 vs. ExPASy Swiss-Prot
Match: Q9M7R0 (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 2.3e-43
Identity = 94/158 (59.49%), Postives = 115/158 (72.78%), Query Frame = 0


Query: 21  SKPSVFLQDNVELRKVFERFDANGDGKISISELDSVLKSLGSAIPYEELRSVMEDLDADK 80
           SKPSV+LQ+  E++ VF RFDANGDGKIS  EL  VLK+LGS    EE+  +ME++D DK
Sbjct: 9   SKPSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDK 68

Query: 81  DGFINIDEFDAFCK---EPMASDEAGVSELRDAFDLYDQDHNGLISHSELHLVLNRLGIS 140
           DGFIN+ EF AF K   +P  S   G +EL++AF+LYDQDHNGLIS  ELH +L RLG  
Sbjct: 69  DGFINVQEFAAFVKAETDPYPS-SGGENELKEAFELYDQDHNGLISSVELHKILTRLGER 128

Query: 141 CSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTTNSNNKA 176
            ++ DC  MI SVDSDGDG V+FEEF+KMMT  S N +
Sbjct: 129 YAEHDCVEMIKSVDSDGDGYVSFEEFKKMMTNKSGNNS 165

[Close]

BLAST of Spg022467 vs. ExPASy Swiss-Prot
Match: Q9C9U8 (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 9.6e-42
Identity = 83/140 (59.29%), Postives = 110/140 (78.57%), Query Frame = 0


Query: 30  NVELRKVFERFDANGDGKISISELDSVLKSLGSAIPYEELRSVMEDLDADKDGFINIDEF 89
           ++EL+KVF++FDANGDGKIS+SEL +V KS+G++   EEL  V++++D D DGFIN +EF
Sbjct: 18  DMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEF 77

Query: 90  DAFCKEPMASDEAGVSELRDAFDLYDQDHNGLISHSELHLVLNRLGISCSKEDCQRMIHS 149
              C+       +   E+R+AFDLYDQ+ NGLIS SE+H VLNRLG++CS EDC RMI  
Sbjct: 78  ATICR-----SSSSAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGH 137

Query: 150 VDSDGDGNVNFEEFRKMMTT 170
           VD+DGDGNVNFEEF+KMM++
Sbjct: 138 VDTDGDGNVNFEEFQKMMSS 152

[Close]

BLAST of Spg022467 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 3.3e-34
Identity = 76/147 (51.70%), Postives = 101/147 (68.71%), Query Frame = 0


Query: 32  ELRKVFERFDANGDGKISISELDSVLKSLGSAIPYEELRSVMEDLDADKDGFINIDEFDA 91
           EL +VF R+DANGDGKIS  EL SVL++LG+ +   E+R +M+++D+D+DGF+++ EF A
Sbjct: 5   ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFAA 64

Query: 92  FCKEPMAS-----------DEAGVSELRDAFDLYDQDHNGLISHSELHLVLNRLGISCSK 151
           F   P  +           + A  +ELR+AF +YD D NG IS  ELH VL +LG  CS 
Sbjct: 65  FHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCSV 124

Query: 152 EDCQRMIHSVDSDGDGNVNFEEFRKMM 168
            DC RMI SVD+DGDG VNF+EF+KMM
Sbjct: 125 ADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]

BLAST of Spg022467 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 5.6e-34
Identity = 73/142 (51.41%), Postives = 101/142 (71.13%), Query Frame = 0


Query: 32  ELRKVFERFDANGDGKISISELDSVLKSLGSAIPYEELRSVMEDLDADKDGFINIDEFDA 91
           +++KVF+RFD N DGKISI EL  V+ +L      EE +++M++ D D +GFI++DEF A
Sbjct: 15  DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVA 74

Query: 92  -FCKEPMASDEAGVSELRDAFDLYDQDHNGLISHSELHLVLNRLGISCSKEDCQRMIHSV 151
            F     +S+ + + +L++AFDLYD D NG IS +ELH V+  LG  CS +DCQRMI+ V
Sbjct: 75  LFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKV 134

Query: 152 DSDGDGNVNFEEFRKMMTTNSN 173
           DSDGDG V+FEEF+KMM  N +
Sbjct: 135 DSDGDGCVDFEEFKKMMMINGS 156

[Close]

BLAST of Spg022467 vs. ExPASy TrEMBL
Match: A0A6J1F164 (probable calcium-binding protein CML27 OS=Cucurbita moschata OX=3662 GN=LOC111441185 PE=4 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 1.2e-82
Identity = 157/186 (84.41%), Postives = 171/186 (91.94%), Query Frame = 0


Query: 1   MATDPNPIPASSSDADQVSKSKPSVFLQDNVELRKVFERFDANGDGKISISELDSVLKSL 60
           MATD N I AS++D +QV KSKPS  LQDN ELRKVF+RFDANGDGKIS+SELDSVLKSL
Sbjct: 1   MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60

Query: 61  GSAIPYEELRSVMEDLDADKDGFINIDEFDAFCKEPMASDEAGVSELRDAFDLYDQDHNG 120
           GS++PY+ELR+VMEDLD DKDGFINIDEF  FCKEPMAS+EAG SELRDAFDLYDQDHNG
Sbjct: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG 120

Query: 121 LISHSELHLVLNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTTNSNNKAPNQNG 180
           LIS SELHLVLNRLGI+CSKEDCQRMIHSVDSDGDGNVNFEEFRKMM+ NSN++APNQNG
Sbjct: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNG 180

Query: 181 TAAAAP 187
           TAA AP
Sbjct: 181 TAATAP 186

[Close]

BLAST of Spg022467 vs. ExPASy TrEMBL
Match: A0A6J1KUB4 (probable calcium-binding protein CML27 OS=Cucurbita maxima OX=3661 GN=LOC111497259 PE=4 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 3.7e-81
Identity = 155/186 (83.33%), Postives = 169/186 (90.86%), Query Frame = 0


Query: 1   MATDPNPIPASSSDADQVSKSKPSVFLQDNVELRKVFERFDANGDGKISISELDSVLKSL 60
           MATD N I AS+SD +QV KSKPS  LQDN ELRKVF+RFDANGDGKIS+SELDSVLKS 
Sbjct: 1   MATDLNQIAASNSDTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSS 60

Query: 61  GSAIPYEELRSVMEDLDADKDGFINIDEFDAFCKEPMASDEAGVSELRDAFDLYDQDHNG 120
           GS++PY+ELR+VMEDLD DKDGFINIDEF  FCKEP+AS+EAG SELRDAFDLYDQDHNG
Sbjct: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPIASNEAGASELRDAFDLYDQDHNG 120

Query: 121 LISHSELHLVLNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTTNSNNKAPNQNG 180
           LIS SELHLVLNRLGI+CSKEDCQRMIHSVDSDGDGNVNFEEFRKMM+ NSN++ PNQNG
Sbjct: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEVPNQNG 180

Query: 181 TAAAAP 187
           TAA AP
Sbjct: 181 TAATAP 186

[Close]

BLAST of Spg022467 vs. ExPASy TrEMBL
Match: A0A6J1E0A3 (probable calcium-binding protein CML27 OS=Cucurbita moschata OX=3662 GN=LOC111429663 PE=4 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 9.2e-80
Identity = 154/186 (82.80%), Postives = 169/186 (90.86%), Query Frame = 0


Query: 1   MATDPNPIPASSSDADQVSKSKPSVFLQDNVELRKVFERFDANGDGKISISELDSVLKSL 60
           MATD NPI +S+SDADQ++K KPS  L +N ELRKVF+RFD+NGDG+ISI+ELDSVLKSL
Sbjct: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60

Query: 61  GSAIPYEELRSVMEDLDADKDGFINIDEFDAFCKEPMASDEAGVSELRDAFDLYDQDHNG 120
           GSAIPYEELR VMEDLD DKDGFINIDEF AFCKEPMASDEAG+SELRDAFDLYDQDHNG
Sbjct: 61  GSAIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120

Query: 121 LISHSELHLVLNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTTNSNNKAPNQNG 180
           LISHSELHLVL RLGI+ S EDCQRMIH+VDSDGDGNVNFEEFRKMM+  + +KAPNQNG
Sbjct: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQNG 180

Query: 181 TAAAAP 187
           TAAA P
Sbjct: 181 TAAAGP 186

[Close]

BLAST of Spg022467 vs. ExPASy TrEMBL
Match: A0A6J1JD54 (probable calcium-binding protein CML27 OS=Cucurbita maxima OX=3661 GN=LOC111485714 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.2e-79
Identity = 153/186 (82.26%), Postives = 169/186 (90.86%), Query Frame = 0


Query: 1   MATDPNPIPASSSDADQVSKSKPSVFLQDNVELRKVFERFDANGDGKISISELDSVLKSL 60
           MATD NPIP+S+SDADQ++K KPS  L +N ELRKVF+RFD+NGDG+ISI+ELDSVLKSL
Sbjct: 1   MATDSNPIPSSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60

Query: 61  GSAIPYEELRSVMEDLDADKDGFINIDEFDAFCKEPMASDEAGVSELRDAFDLYDQDHNG 120
           GSAIPYEELR +MEDLD DKDGFINIDEF AFCKEPMASDEAG+SELRDAFDLYDQDHNG
Sbjct: 61  GSAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120

Query: 121 LISHSELHLVLNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTTNSNNKAPNQNG 180
           LISHSELHLVL RLGI+ S EDCQRMIH+VDSDGDGNVNFEEFRKMM+  + +KAPNQN 
Sbjct: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPNQNA 180

Query: 181 TAAAAP 187
           TAAA P
Sbjct: 181 TAAAGP 186

[Close]

BLAST of Spg022467 vs. ExPASy TrEMBL
Match: A0A0A0LPP8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025020 PE=4 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 7.8e-79
Identity = 156/188 (82.98%), Postives = 169/188 (89.89%), Query Frame = 0


Query: 1   MATDPNPIPASSSDADQVSKSKPSVFLQDNVELRKVFERFDANGDGKISISELDSVLKS- 60
           MATDPNPIP S S+ADQ++K  PSV LQD+ ELRKVFERFDANGDGKISISELD+VL S 
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  -LGSAIPYEELRSVMEDLDADKDGFINIDEFDAFCKEPMASDEAGVSELRDAFDLYDQDH 120
            L SAIP EELRSVM+DLD+DKDG+INIDEF AFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTTNSNNKAPNQ 180
           NGLIS SELHLVLNRLGISCSKEDCQ+MI+SVDSDGDGNVNFEEFRKMMT NS +KA  Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NGTAAAAP 187
           NGTAAAAP
Sbjct: 181 NGTAAAAP 188

[Close]

BLAST of Spg022467 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 181.0 bits (458), Expect = 8.6e-46
Identity = 93/173 (53.76%), Postives = 126/173 (72.83%), Query Frame = 0


Query: 11  SSSDADQVSKSKPSVFLQDNVELRKVFERFDANGDGKISISELDSVLKSLGSAIPYEELR 70
           +S++ +    +  +V + +  EL+KVF++FD+NGDGKIS+ EL  V K++G++    EL 
Sbjct: 2   ASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELN 61

Query: 71  SVMEDLDADKDGFINIDEFDAFCKEPMASDEAGVSELRDAFDLYDQDHNGLISHSELHLV 130
            V+E++D D+DG+IN+DEF   C+       +  +E+RDAFDLYDQD NGLIS SELH V
Sbjct: 62  RVLEEVDTDRDGYINLDEFSTLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQV 121

Query: 131 LNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTTNSNNKAPNQNGTAA 184
           LNRLG+SCS EDC RMI  VD+DGDGNVNFEEF+KMMT++S     N NG+AA
Sbjct: 122 LNRLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS---LLNSNGSAA 166

[Close]

BLAST of Spg022467 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 181.0 bits (458), Expect = 8.6e-46
Identity = 93/173 (53.76%), Postives = 126/173 (72.83%), Query Frame = 0


Query: 11  SSSDADQVSKSKPSVFLQDNVELRKVFERFDANGDGKISISELDSVLKSLGSAIPYEELR 70
           +S++ +    +  +V + +  EL+KVF++FD+NGDGKIS+ EL  V K++G++    EL 
Sbjct: 2   ASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELN 61

Query: 71  SVMEDLDADKDGFINIDEFDAFCKEPMASDEAGVSELRDAFDLYDQDHNGLISHSELHLV 130
            V+E++D D+DG+IN+DEF   C+       +  +E+RDAFDLYDQD NGLIS SELH V
Sbjct: 62  RVLEEVDTDRDGYINLDEFSTLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQV 121

Query: 131 LNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTTNSNNKAPNQNGTAA 184
           LNRLG+SCS EDC RMI  VD+DGDGNVNFEEF+KMMT++S     N NG+AA
Sbjct: 122 LNRLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS---LLNSNGSAA 166

[Close]

BLAST of Spg022467 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 171.4 bits (433), Expect = 6.8e-43
Identity = 83/140 (59.29%), Postives = 110/140 (78.57%), Query Frame = 0


Query: 30  NVELRKVFERFDANGDGKISISELDSVLKSLGSAIPYEELRSVMEDLDADKDGFINIDEF 89
           ++EL+KVF++FDANGDGKIS+SEL +V KS+G++   EEL  V++++D D DGFIN +EF
Sbjct: 18  DMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEF 77

Query: 90  DAFCKEPMASDEAGVSELRDAFDLYDQDHNGLISHSELHLVLNRLGISCSKEDCQRMIHS 149
              C+       +   E+R+AFDLYDQ+ NGLIS SE+H VLNRLG++CS EDC RMI  
Sbjct: 78  ATICR-----SSSSAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGH 137

Query: 150 VDSDGDGNVNFEEFRKMMTT 170
           VD+DGDGNVNFEEF+KMM++
Sbjct: 138 VDTDGDGNVNFEEFQKMMSS 152

[Close]

BLAST of Spg022467 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 145.6 bits (366), Expect = 4.0e-35
Identity = 73/142 (51.41%), Postives = 101/142 (71.13%), Query Frame = 0


Query: 32  ELRKVFERFDANGDGKISISELDSVLKSLGSAIPYEELRSVMEDLDADKDGFINIDEFDA 91
           +++KVF+RFD N DGKISI EL  V+ +L      EE +++M++ D D +GFI++DEF A
Sbjct: 15  DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVA 74

Query: 92  -FCKEPMASDEAGVSELRDAFDLYDQDHNGLISHSELHLVLNRLGISCSKEDCQRMIHSV 151
            F     +S+ + + +L++AFDLYD D NG IS +ELH V+  LG  CS +DCQRMI+ V
Sbjct: 75  LFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKV 134

Query: 152 DSDGDGNVNFEEFRKMMTTNSN 173
           DSDGDG V+FEEF+KMM  N +
Sbjct: 135 DSDGDGCVDFEEFKKMMMINGS 156

[Close]

BLAST of Spg022467 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 135.6 bits (340), Expect = 4.1e-32
Identity = 68/140 (48.57%), Postives = 97/140 (69.29%), Query Frame = 0


Query: 32  ELRKVFERFDANGDGKISISELDSVLKSLGSAIPYEELRSVMEDLDADKDGFINIDEFDA 91
           +++KVF+RFD NGDGKIS+ EL  V+++L      EE  ++M+  D D +GFI++DEF A
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 92  FCKEPM---ASDEAGVSELRDAFDLYDQDHNGLISHSELHLVLNRLGISCSKEDCQRMIH 151
             +  +    ++   VS+L++AF+LYD D NG IS  ELH V+  LG  CS +DC++MI 
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 152 SVDSDGDGNVNFEEFRKMMT 169
            VD DGDG VNF+EF+KMM+
Sbjct: 137 KVDIDGDGCVNFDEFKKMMS 156

The following BLAST results are available for this feature:

BLAST of Spg022467 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878467.1	1.4e-90	93.55	probable calcium-binding protein CML27 [Benincasa hispida]	[more]
XP_023529417.1	1.1e-82	84.95	probable calcium-binding protein CML27 [Cucurbita pepo subsp. pepo]	[more]
XP_022933917.1	2.4e-82	84.41	probable calcium-binding protein CML27 [Cucurbita moschata] >KAG7021647.1 putati...	[more]
KAG6587683.1	2.7e-81	83.87	putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023003784.1	7.7e-81	83.33	probable calcium-binding protein CML27 [Cucurbita maxima]	[more]

BLAST of Spg022467 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LE22	1.2e-44	53.76	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]
Q9M7R0	2.3e-43	59.49	Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
Q9C9U8	9.6e-42	59.29	Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 ...	[more]
Q0DJV6	3.3e-34	51.70	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9C8Y1	5.6e-34	51.41	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]

BLAST of Spg022467 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F164	1.2e-82	84.41	probable calcium-binding protein CML27 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1KUB4	3.7e-81	83.33	probable calcium-binding protein CML27 OS=Cucurbita maxima OX=3661 GN=LOC1114972...	[more]
A0A6J1E0A3	9.2e-80	82.80	probable calcium-binding protein CML27 OS=Cucurbita moschata OX=3662 GN=LOC11142...	[more]
A0A6J1JD54	1.2e-79	82.26	probable calcium-binding protein CML27 OS=Cucurbita maxima OX=3661 GN=LOC1114857...	[more]
A0A0A0LPP8	7.8e-79	82.98	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025020 PE=4 SV=1	[more]

BLAST of Spg022467 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18210.1	8.6e-46	53.76	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	8.6e-46	53.76	Calcium-binding EF-hand family protein	[more]
AT1G73630.1	6.8e-43	59.29	EF hand calcium-binding protein family	[more]
AT1G66400.1	4.0e-35	51.41	calmodulin like 23	[more]
AT5G37770.1	4.1e-32	48.57	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 68..96 e-value: 4.9E-4 score: 29.4 coord: 32..60 e-value: 8.2E-6 score: 35.3 coord: 142..170 e-value: 1.1E-4 score: 31.6 coord: 106..134 e-value: 3.0E-4 score: 30.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 106..167 e-value: 9.8E-14 score: 51.6 coord: 32..93 e-value: 5.2E-12 score: 46.0
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 28..63 score: 14.401823
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 102..137 score: 12.979158
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 64..99 score: 11.640179
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 138..173 score: 13.955498
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 106..168 e-value: 6.72895E-21 score: 79.5141
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 32..89 e-value: 1.28335E-16 score: 68.7285
None	No IPR available	GENE3D	1.10.238.10		coord: 2..100 e-value: 9.8E-21 score: 76.3
None	No IPR available	GENE3D	1.10.238.10		coord: 101..179 e-value: 2.7E-23 score: 84.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 19..171
None	No IPR available	PANTHER	PTHR10891:SF814	CALCIUM-BINDING PROTEIN CML26-RELATED	coord: 19..171
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 77..89
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 41..53
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 151..163
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 115..127
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 32..176

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022467.1	Spg022467.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding