Spg022300 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg022300 vs. NCBI nr
Match: XP_022969128.1 (protein PHOX1-like [Cucurbita maxima] >XP_022969137.1 protein PHOX1-like [Cucurbita maxima] >XP_022969145.1 protein PHOX1-like [Cucurbita maxima])

HSP 1 Score: 1253.4 bits (3242), Expect = 0.0e+00
Identity = 654/764 (85.60%), Postives = 687/764 (89.92%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNTLFQSRD 60
           MGKQ GKKKKQIGDKFRE I+KHRQ GDGSP + DKDH IFITMS  LKEEGN LFQ+RD
Sbjct: 1   MGKQSGKKKKQIGDKFREVIAKHRQSGDGSPSNNDKDHAIFITMSLALKEEGNRLFQARD 60

Query: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALEVTPKYSK 120
           LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMG+SEYPRAIHECNLALEVTPKYSK
Sbjct: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGISEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSKDVDAEIKL 180
           ALLKRARCYEALHRLDLALRDVN VLNMEP NIMALEISERLT+ALETKGSK+ DAEIKL
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNTVLNMEPNNIMALEISERLTKALETKGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKA------------D 240
           PLDFVELPA VLPQK  KEK+RKKKNNQKTKETIDEKKADEIVEEK             +
Sbjct: 181 PLDFVELPAEVLPQKGSKEKNRKKKNNQKTKETIDEKKADEIVEEKVGNETVEVEKAGNE 240

Query: 241 EIVEEKAGDETV-----------QEKADKIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQ 300
            + EEKAG+ETV           +EKA    VEEEKA+ET EEKKAEDKLVVEEKIA+  
Sbjct: 241 TVEEEKAGNETVEEETAGNGTVAEEKAGNGTVEEEKAKETVEEKKAEDKLVVEEKIARAL 300

Query: 301 EEIPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITT 360
           EE PMKTVKLVF EDIRWAQ+P+ CTLLQLRE IRDRFPT  AVLIKYRDEEGDLVTIT 
Sbjct: 301 EETPMKTVKLVFGEDIRWAQVPVDCTLLQLREAIRDRFPTCPAVLIKYRDEEGDLVTITA 360

Query: 361 NEELRLAETSTESQGSVRFYIFEVSPEKDPFYERL-NNNEVAKCKVEQSSVFENGHVLRA 420
           NEELR+AET+  SQ SVRFYIFEV PE+DPFYE+   ++EVAKCKVEQSS+FENGH+LRA
Sbjct: 361 NEELRMAETAKASQASVRFYIFEVKPEQDPFYEKFKKDDEVAKCKVEQSSIFENGHLLRA 420

Query: 421 KEMKMSSCIDDWIIHFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFD 480
           KE+K SSCIDDWII FAQLFINHVGFESGP LDLHDLGMKLY+EAVEETVTSEEAQGLF 
Sbjct: 421 KEIKTSSCIDDWIIQFAQLFINHVGFESGPCLDLHDLGMKLYSEAVEETVTSEEAQGLFK 480

Query: 481 LAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGR 540
           LAAEKFHEM ALALFYWGNVIMS ARKKVYFTDGASK PLF+QIK+AYDWVE EY EAGR
Sbjct: 481 LAAEKFHEMTALALFYWGNVIMSSARKKVYFTDGASKGPLFEQIKSAYDWVENEYVEAGR 540

Query: 541 IYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNM 600
            YQMA+EIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVD KTLPCTEVLKLYN AE++M
Sbjct: 541 KYQMAVEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDPKTLPCTEVLKLYNYAEESM 600

Query: 601 ETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILW 660
           ETGMKMWEEWEEQRT EFSK+S VKTQLQKMGLDGLIKDISA EAAEQAKNMRSHIN+LW
Sbjct: 601 ETGMKMWEEWEEQRTSEFSKTSTVKTQLQKMGLDGLIKDISADEAAEQAKNMRSHINLLW 660

Query: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720
           GTMLYERSILEFKMG+PVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK
Sbjct: 661 GTMLYERSILEFKMGIPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720

Query: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 741
           IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA
Sbjct: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 764

BLAST of Spg022300 vs. NCBI nr
Match: KAG6587865.1 (Protein PHOX1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021753.1 Protein PHOX1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1249.2 bits (3231), Expect = 0.0e+00
Identity = 653/764 (85.47%), Postives = 686/764 (89.79%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNTLFQSRD 60
           MGKQ GKKKKQIGDKFRE I+KHRQ GDGSP + DKDH IFITMS  LKEEGN LFQ+RD
Sbjct: 1   MGKQSGKKKKQIGDKFREVIAKHRQSGDGSPSNNDKDHAIFITMSLALKEEGNRLFQARD 60

Query: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALEVTPKYSK 120
           LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMG+SEYPRAIHECNLALEVTPKYSK
Sbjct: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGISEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSKDVDAEIKL 180
           ALLKRARCYEALHRLDLALRDVN VLNMEP NIMALEISERLT+ALETKGSK+ DAEIKL
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNTVLNMEPNNIMALEISERLTKALETKGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKA------------D 240
           PLDFVELPA VLPQK  KEK+RKKKNNQKTKETI EKKADEIVEEK             +
Sbjct: 181 PLDFVELPAEVLPQKGSKEKNRKKKNNQKTKETIIEKKADEIVEEKVGNETVEVEKAGNE 240

Query: 241 EIVEEKAGDETV-----------QEKADKIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQ 300
            + EEKAG+ETV           +EKA    VEEEKA+E+ EEKKAEDKLVVEEKIA+  
Sbjct: 241 TVEEEKAGNETVEEETAGNGTVAEEKAGNGTVEEEKAKESVEEKKAEDKLVVEEKIARAL 300

Query: 301 EEIPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITT 360
           EE PMKTVKLVF EDIRWAQ+P+ CTLLQLRE IRDRFPT  AVLIKYRDEEGDLVTIT 
Sbjct: 301 EETPMKTVKLVFGEDIRWAQVPVDCTLLQLREAIRDRFPTCPAVLIKYRDEEGDLVTITA 360

Query: 361 NEELRLAETSTESQGSVRFYIFEVSPEKDPFYERL-NNNEVAKCKVEQSSVFENGHVLRA 420
           NEELR+AET+  SQ SVRFYIFEV PE+DPFYE+   ++EVAKCKVEQSS+FENGH+LRA
Sbjct: 361 NEELRMAETAKASQASVRFYIFEVKPEQDPFYEKFKKDDEVAKCKVEQSSIFENGHLLRA 420

Query: 421 KEMKMSSCIDDWIIHFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFD 480
           KE+K SSCIDDWII FAQLFINHVGFESGP LDLHDLGMKLY+EAVEETVTSEEAQGLF 
Sbjct: 421 KEIKTSSCIDDWIIQFAQLFINHVGFESGPCLDLHDLGMKLYSEAVEETVTSEEAQGLFK 480

Query: 481 LAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGR 540
           LAAEKFHEM ALALFYWGNVIMS ARKKVYFTDGASK PLF+QIK+AYDWVE EY EAGR
Sbjct: 481 LAAEKFHEMTALALFYWGNVIMSSARKKVYFTDGASKGPLFEQIKSAYDWVENEYVEAGR 540

Query: 541 IYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNM 600
            YQMA+EIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVD KTLPCTEVLKLYN AE++M
Sbjct: 541 KYQMAVEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDPKTLPCTEVLKLYNYAEESM 600

Query: 601 ETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILW 660
           ETGMKMWEEWEEQRT EFSK+S VKTQLQKMGLDGLIKDISA EAAEQAKNMRSHIN+LW
Sbjct: 601 ETGMKMWEEWEEQRTSEFSKTSTVKTQLQKMGLDGLIKDISADEAAEQAKNMRSHINLLW 660

Query: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720
           GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK
Sbjct: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720

Query: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 741
           IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA
Sbjct: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 764

BLAST of Spg022300 vs. NCBI nr
Match: XP_022932878.1 (protein PHOX1-like [Cucurbita moschata] >XP_022932887.1 protein PHOX1-like [Cucurbita moschata] >XP_022932896.1 protein PHOX1-like [Cucurbita moschata])

HSP 1 Score: 1248.4 bits (3229), Expect = 0.0e+00
Identity = 652/764 (85.34%), Postives = 686/764 (89.79%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNTLFQSRD 60
           MGKQ GKKKKQIGDKFRE I+KHRQ GDGSP + DKDH IFITMS  LKEEGN LFQ+RD
Sbjct: 1   MGKQSGKKKKQIGDKFREVIAKHRQSGDGSPSNNDKDHAIFITMSLALKEEGNRLFQARD 60

Query: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALEVTPKYSK 120
           LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMG+SEYPRAIHECNLALEVTPKYSK
Sbjct: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGISEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSKDVDAEIKL 180
           ALLKRARCYEALHRLDLALRDVN VLNMEP NIMALEISERLT+ALETKGSK+ DAEIKL
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNTVLNMEPNNIMALEISERLTKALETKGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKA------------D 240
           PLDFVELPA VLPQK  KEK+RKKKNNQKTKETI EKKADEIVEEK             +
Sbjct: 181 PLDFVELPAEVLPQKGSKEKNRKKKNNQKTKETIIEKKADEIVEEKVGNETVEVEKAGNE 240

Query: 241 EIVEEKAGDETV-----------QEKADKIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQ 300
            + EEKAG+ETV           +EKA    VEEEKA+E+ EEKKAEDKLVVEEKIA+  
Sbjct: 241 TVEEEKAGNETVEEETAGNGTVAEEKAGNGTVEEEKAKESVEEKKAEDKLVVEEKIARAL 300

Query: 301 EEIPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITT 360
           EE PMKTVKLVF EDIRWAQ+P+ CTLLQLRE IRDRFPT  AVLIKYRDEEGDLVTIT 
Sbjct: 301 EETPMKTVKLVFGEDIRWAQVPVDCTLLQLREAIRDRFPTCPAVLIKYRDEEGDLVTITA 360

Query: 361 NEELRLAETSTESQGSVRFYIFEVSPEKDPFYERL-NNNEVAKCKVEQSSVFENGHVLRA 420
           NEELR+AET+  SQ SVRFYIFEV PE+DPFYE+   ++EVAKCKVEQSS+FENGH+LRA
Sbjct: 361 NEELRMAETAKASQASVRFYIFEVKPEQDPFYEKFKKDDEVAKCKVEQSSIFENGHLLRA 420

Query: 421 KEMKMSSCIDDWIIHFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFD 480
           KE+K SSCIDDWII FAQLFINHVGFESGP LDLHDLGMKLY+EAVEETVTSEEAQGLF 
Sbjct: 421 KEIKTSSCIDDWIIQFAQLFINHVGFESGPCLDLHDLGMKLYSEAVEETVTSEEAQGLFK 480

Query: 481 LAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGR 540
           LAAEKFHEM ALALFYWGNVIMS ARKKVYFTDGASK PLF+Q+K+AYDWVE EY EAGR
Sbjct: 481 LAAEKFHEMTALALFYWGNVIMSSARKKVYFTDGASKEPLFEQVKSAYDWVENEYVEAGR 540

Query: 541 IYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNM 600
            YQMA+EIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVD KTLPCTEVLKLYN AE++M
Sbjct: 541 KYQMAVEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDPKTLPCTEVLKLYNYAEESM 600

Query: 601 ETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILW 660
           ETGMKMWEEWEEQRT EFSK+S VKTQLQKMGLDGLIKDISA EAAEQAKNMRSHIN+LW
Sbjct: 601 ETGMKMWEEWEEQRTSEFSKTSTVKTQLQKMGLDGLIKDISADEAAEQAKNMRSHINLLW 660

Query: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720
           GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK
Sbjct: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720

Query: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 741
           IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA
Sbjct: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 764

BLAST of Spg022300 vs. NCBI nr
Match: XP_023530345.1 (protein PHOX1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1245.7 bits (3222), Expect = 0.0e+00
Identity = 650/764 (85.08%), Postives = 685/764 (89.66%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNTLFQSRD 60
           MGKQ GKKKKQIGDKFRE I+KHRQ GDGSP + DKDH IFITMS  LKEEGN LFQ+RD
Sbjct: 1   MGKQSGKKKKQIGDKFREVIAKHRQSGDGSPSNNDKDHAIFITMSLALKEEGNRLFQARD 60

Query: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALEVTPKYSK 120
           LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMG+SEYPRAIHECNLALEVTPKYSK
Sbjct: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGISEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSKDVDAEIKL 180
           ALLKRARCYEALHRLDLALRDVN VLNMEP NIMALEISERLT+ALETKGSK+ DAEIKL
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNTVLNMEPNNIMALEISERLTKALETKGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKA------------D 240
           PLDFVELPA VLPQK  KEK+RKKKNNQKTKETIDEKKADEIVEEK             +
Sbjct: 181 PLDFVELPAEVLPQKGSKEKNRKKKNNQKTKETIDEKKADEIVEEKVGNETVEVEKAGNE 240

Query: 241 EIVEEKAGDETV-----------QEKADKIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQ 300
            + EEKAG+ETV           +EKA    VEEEKA+E+ EEKKAEDKLVVEEKIA+  
Sbjct: 241 TVEEEKAGNETVEEETAGNGTVAEEKAGNGTVEEEKAKESVEEKKAEDKLVVEEKIARAL 300

Query: 301 EEIPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITT 360
           EE PMKTVKLVF EDIRWAQ+P+ CTLLQLRE IRDRFPT  AVLIKYRDEEGDLVTIT 
Sbjct: 301 EETPMKTVKLVFGEDIRWAQVPVDCTLLQLREAIRDRFPTCPAVLIKYRDEEGDLVTITA 360

Query: 361 NEELRLAETSTESQGSVRFYIFEVSPEKDPFYERL-NNNEVAKCKVEQSSVFENGHVLRA 420
           NEELR+AET+  SQ SVRFYIFEV PE+DPFYE+   ++EVAKCKVEQSS+FENGH+LRA
Sbjct: 361 NEELRMAETAKASQASVRFYIFEVKPEQDPFYEKFKKDDEVAKCKVEQSSIFENGHLLRA 420

Query: 421 KEMKMSSCIDDWIIHFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFD 480
           KE+K SSCIDDWII FAQLFINHVGFESGP LDLHDLGMKLY+EAVEETVTSEEAQGLF 
Sbjct: 421 KEIKTSSCIDDWIIQFAQLFINHVGFESGPCLDLHDLGMKLYSEAVEETVTSEEAQGLFK 480

Query: 481 LAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGR 540
           LAAEKFHEM ALALFYWGNVIMS ARKKVYFTDGASK PLF+QIK+AYDWVE +Y EAGR
Sbjct: 481 LAAEKFHEMTALALFYWGNVIMSSARKKVYFTDGASKGPLFEQIKSAYDWVENKYVEAGR 540

Query: 541 IYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNM 600
            YQMA+EIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVD KTL CTEVLKLYN AE++M
Sbjct: 541 KYQMAVEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDPKTLSCTEVLKLYNYAEESM 600

Query: 601 ETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILW 660
           ETGMKMWEEWEEQRT EFSK+S VKTQLQKMGLDGLIKDISA EA EQAKNMRSHIN+LW
Sbjct: 601 ETGMKMWEEWEEQRTSEFSKTSTVKTQLQKMGLDGLIKDISADEATEQAKNMRSHINLLW 660

Query: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720
           GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEG+GFK
Sbjct: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGIGFK 720

Query: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 741
           IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA
Sbjct: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 764

BLAST of Spg022300 vs. NCBI nr
Match: XP_023530344.1 (protein PHOX1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1241.9 bits (3212), Expect = 0.0e+00
Identity = 650/774 (83.98%), Postives = 685/774 (88.50%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNTLFQSRD 60
           MGKQ GKKKKQIGDKFRE I+KHRQ GDGSP + DKDH IFITMS  LKEEGN LFQ+RD
Sbjct: 1   MGKQSGKKKKQIGDKFREVIAKHRQSGDGSPSNNDKDHAIFITMSLALKEEGNRLFQARD 60

Query: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALEVTPKYSK 120
           LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMG+SEYPRAIHECNLALEVTPKYSK
Sbjct: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGISEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSKDVDAEIKL 180
           ALLKRARCYEALHRLDLALRDVN VLNMEP NIMALEISERLT+ALETKGSK+ DAEIKL
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNTVLNMEPNNIMALEISERLTKALETKGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKA------------D 240
           PLDFVELPA VLPQK  KEK+RKKKNNQKTKETIDEKKADEIVEEK             +
Sbjct: 181 PLDFVELPAEVLPQKGSKEKNRKKKNNQKTKETIDEKKADEIVEEKVGNETVEVEKAGNE 240

Query: 241 EIVEEKAGDETV---------------------QEKADKIIVEEEKAEETGEEKKAEDKL 300
            + EEKAG+ETV                     +EKA    VEEEKA+E+ EEKKAEDKL
Sbjct: 241 TVEEEKAGNETVEEETAGNGTVAEEKAGNGTVEEEKAGNGTVEEEKAKESVEEKKAEDKL 300

Query: 301 VVEEKIAKTQEEIPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRD 360
           VVEEKIA+  EE PMKTVKLVF EDIRWAQ+P+ CTLLQLRE IRDRFPT  AVLIKYRD
Sbjct: 301 VVEEKIARALEETPMKTVKLVFGEDIRWAQVPVDCTLLQLREAIRDRFPTCPAVLIKYRD 360

Query: 361 EEGDLVTITTNEELRLAETSTESQGSVRFYIFEVSPEKDPFYERL-NNNEVAKCKVEQSS 420
           EEGDLVTIT NEELR+AET+  SQ SVRFYIFEV PE+DPFYE+   ++EVAKCKVEQSS
Sbjct: 361 EEGDLVTITANEELRMAETAKASQASVRFYIFEVKPEQDPFYEKFKKDDEVAKCKVEQSS 420

Query: 421 VFENGHVLRAKEMKMSSCIDDWIIHFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETV 480
           +FENGH+LRAKE+K SSCIDDWII FAQLFINHVGFESGP LDLHDLGMKLY+EAVEETV
Sbjct: 421 IFENGHLLRAKEIKTSSCIDDWIIQFAQLFINHVGFESGPCLDLHDLGMKLYSEAVEETV 480

Query: 481 TSEEAQGLFDLAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDW 540
           TSEEAQGLF LAAEKFHEM ALALFYWGNVIMS ARKKVYFTDGASK PLF+QIK+AYDW
Sbjct: 481 TSEEAQGLFKLAAEKFHEMTALALFYWGNVIMSSARKKVYFTDGASKGPLFEQIKSAYDW 540

Query: 541 VEKEYAEAGRIYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVL 600
           VE +Y EAGR YQMA+EIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVD KTL CTEVL
Sbjct: 541 VENKYVEAGRKYQMAVEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDPKTLSCTEVL 600

Query: 601 KLYNNAEDNMETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAK 660
           KLYN AE++METGMKMWEEWEEQRT EFSK+S VKTQLQKMGLDGLIKDISA EA EQAK
Sbjct: 601 KLYNYAEESMETGMKMWEEWEEQRTSEFSKTSTVKTQLQKMGLDGLIKDISADEATEQAK 660

Query: 661 NMRSHINILWGTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSS 720
           NMRSHIN+LWGTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSS
Sbjct: 661 NMRSHINLLWGTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSS 720

Query: 721 NNAHEGLGFKIDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 741
           NNAHEG+GFKIDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA
Sbjct: 721 NNAHEGIGFKIDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 774

BLAST of Spg022300 vs. ExPASy Swiss-Prot
Match: F4IRM4 (Protein PHOX1 OS=Arabidopsis thaliana OX=3702 GN=PHOX1 PE=1 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 3.2e-205
Identity = 406/751 (54.06%), Postives = 526/751 (70.04%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGD-------GSPKSYDKDHVIFITMSQVLKEEGN 60
           MGK  GKKK     +     S    GG         + KS+D D  IFI  +  LKEEGN
Sbjct: 1   MGKPTGKKKNNNYTEMPPTESSTTGGGKTGKSFDRSATKSFDDDMTIFINRALELKEEGN 60

Query: 61  TLFQSRDLEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALE 120
            LFQ RD EGAM +Y+KA+KLLPR+H DV+YLR++MA+CYMQMGL EYP AI+ECNLALE
Sbjct: 61  KLFQKRDYEGAMFRYDKAVKLLPRDHGDVAYLRTSMASCYMQMGLGEYPNAINECNLALE 120

Query: 121 VTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSKD 180
            +P++SKALLKRARCYEAL++LD A RD   VLNMEP+N+ A EI ER+ + L  KG  D
Sbjct: 121 ASPRFSKALLKRARCYEALNKLDFAFRDSRVVLNMEPENVSANEIFERVKKVLVGKGI-D 180

Query: 181 VDAEIKLPLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKADEIVEE 240
           VD E++  L  V+   A   +K  KE+ RKKK     K+++         E K+ E V E
Sbjct: 181 VD-EMEKNLVNVQPVGAARLRKIVKERLRKKK-----KKSMTMTNGGNDGERKSVEAVVE 240

Query: 241 KAGDETVQEKADKIIVEEEKAEETGEEKKAEDKLVVEEK---IAKTQEEIPM-KTVKLVF 300
            A  +  +E      V+  +  +  EEKK EDK+ V +K    ++ +E+  + +TVKLV 
Sbjct: 241 DAKVDNGEE------VDSGRKGKAIEEKKLEDKVAVMDKEVIASEIKEDATVTRTVKLVH 300

Query: 301 EEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAETSTE 360
            +DIRWAQLP+  +++ +R+VI+DRFP     LIKYRD EGDLVTITT +ELRLA ++ E
Sbjct: 301 GDDIRWAQLPLDSSVVLVRDVIKDRFPALKGFLIKYRDSEGDLVTITTTDELRLAASTRE 360

Query: 361 SQGSVRFYIFEVSPEKDPFYERLNNNE-VAKCKVEQSSVFENGHVLRAKEM-KMSSCIDD 420
             GS R YI EVSP ++P Y+ ++N+E   K     SSV +NG V    E  K S+ ++ 
Sbjct: 361 KLGSFRLYIAEVSPNQEPTYDVIDNDESTDKFAKGSSSVADNGSVGDFVESEKASTSLEH 420

Query: 421 WIIHFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHEMAA 480
           WI  FAQLF NHVGF+S  YL+LH+LGMKLYTEA+E+ VT E+AQ LFD+AA+KF EMAA
Sbjct: 421 WIFQFAQLFKNHVGFDSDSYLELHNLGMKLYTEAMEDIVTGEDAQELFDIAADKFQEMAA 480

Query: 481 LALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEIKPD 540
           LA+F WGNV MSKAR+++YF +  S+  + ++++A ++W + EY +A   Y+ A++IK D
Sbjct: 481 LAMFNWGNVHMSKARRQIYFPEDGSRETILEKVEAGFEWAKNEYNKAAEKYEGAVKIKSD 540

Query: 541 FYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNMETGMKMWEEWE 600
           FYE  LALGQQQFEQAKL W +A+S  VD ++    +VLKLYN AE++ME GM++WEE E
Sbjct: 541 FYEALLALGQQQFEQAKLCWYHALSGEVDIESDASQDVLKLYNKAEESMEKGMQIWEEME 600

Query: 601 EQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILWGTMLYERSILE 660
           E+R    S     K  LQK+GLDG+  + S  E+AEQ  NM S IN+LWG++LYERSI+E
Sbjct: 601 ERRLNGISNFDKHKELLQKLGLDGIFSEASDEESAEQTANMSSQINLLWGSLLYERSIVE 660

Query: 661 FKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNEM 720
           +K+GLP W ECLEVAVEKFELAGASATDIAVM+KNHCSS+NA EG+GFKIDEIVQAWNEM
Sbjct: 661 YKLGLPTWDECLEVAVEKFELAGASATDIAVMVKNHCSSDNALEGMGFKIDEIVQAWNEM 720

Query: 721 YEARKLLTGVPSFRLEPLFRRRVSKIYHVLE 739
           Y+A++   GVPSFRLEPLFRRR  K++ +LE
Sbjct: 721 YDAKRWQIGVPSFRLEPLFRRRSPKLHDILE 738

BLAST of Spg022300 vs. ExPASy Swiss-Prot
Match: F4JTI1 (Protein PHOX4 OS=Arabidopsis thaliana OX=3702 GN=PHOX4 PE=2 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 2.1e-201
Identity = 404/781 (51.73%), Postives = 528/781 (67.61%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGDGSPKSY--------DKDHVIFITMSQVLKEEG 60
           MGK   KKK    +  ++A      GG  S K+Y        D+D  IFI+ +  LKEEG
Sbjct: 1   MGKPTAKKKNP--ETPKDASGGGGGGGGKSGKTYHRSTSRVFDEDMEIFISRALELKEEG 60

Query: 61  NTLFQSRDLEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLAL 120
           N LFQ RD EGAML ++KALKLLP++HIDV+YLR++MA+CYMQMGL EYP AI ECNLAL
Sbjct: 61  NKLFQKRDHEGAMLSFDKALKLLPKDHIDVAYLRTSMASCYMQMGLGEYPNAISECNLAL 120

Query: 121 EVTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSK 180
           E +P+YSKAL++R+RCYEAL++LD A RD   VLNMEP N+ A EI +R+ + L  KG  
Sbjct: 121 EASPRYSKALVRRSRCYEALNKLDYAFRDARIVLNMEPGNVSANEIFDRVKKVLVDKGI- 180

Query: 181 DVDAEIKLPLDFVELP--AAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIV------- 240
           DVD   ++  DFV++    A   +K  KE+ RK K  +K+    +E K+ ++V       
Sbjct: 181 DVD---EMEKDFVDVQPVCAARLKKIVKERLRKSKKKKKSGGKDEELKSPKVVVVDKGDE 240

Query: 241 --------EEKADEI-VEEKAGDETVQE----KADKIIVEEEKAEETGEEKKAEDKLVVE 300
                   EEK+D+  ++ K G +  ++    K+DK   ++    + GEE+K EDK+VV 
Sbjct: 241 AEGRNKPKEEKSDKSDIDGKIGGKREEKKTSFKSDKGQKKKSGGNKAGEERKVEDKVVVM 300

Query: 301 EKI-----------AKTQEEIPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTST 360
           +K            +K +     +T+KLV  +DIRWAQLP+  T+  +R+VIRDRFP   
Sbjct: 301 DKEVIASEIVDGGGSKKEGATVTRTIKLVHGDDIRWAQLPLDSTVRLVRDVIRDRFPALR 360

Query: 361 AVLIKYRDEEGDLVTITTNEELRLAETSTESQGSVRFYIFEVSPEKDPFYERLNNNE-VA 420
             LIKYRD EGDLVTITT +ELRLA ++ +  GS+R YI EV+P+++P Y+ ++N E   
Sbjct: 361 GFLIKYRDTEGDLVTITTTDELRLAASTHDKLGSLRLYIAEVNPDQEPTYDGMSNTESTD 420

Query: 421 KCKVEQSSVFENGHVLR-AKEMKMSSCIDDWIIHFAQLFINHVGFESGPYLDLHDLGMKL 480
           K     SS+ +NG V       K S C ++WI  FAQLF NHVGF+S  Y+DLHDLGMKL
Sbjct: 421 KVSKRLSSLADNGSVGEYVGSDKASGCFENWIFQFAQLFKNHVGFDSDSYVDLHDLGMKL 480

Query: 481 YTEAVEETVTSEEAQGLFDLAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLF 540
           YTEA+E+ VT E+AQ LF +AA+KF EM ALAL  WGNV MSKARK+V   + AS+  + 
Sbjct: 481 YTEAMEDAVTGEDAQELFQIAADKFQEMGALALLNWGNVHMSKARKQVCIPEDASREAII 540

Query: 541 DQIKAAYDWVEKEYAEAGRIYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDS 600
           + ++AA+ W + EY +A   Y+ AI++KPDFYE  LALGQ+QFE AKL W +A+ S VD 
Sbjct: 541 EAVEAAFVWTQNEYNKAAEKYEEAIKVKPDFYEALLALGQEQFEHAKLCWYHALKSKVDL 600

Query: 601 KTLPCTEVLKLYNNAEDNMETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDIS 660
           ++    EVLKLYN AED+ME GM++WEE EE R    SK    K  L+K+ LD L  + S
Sbjct: 601 ESEASQEVLKLYNKAEDSMERGMQIWEEMEECRLNGISKLDKHKNMLRKLELDELFSEAS 660

Query: 661 AAEAAEQAKNMRSHINILWGTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIA 720
             E  EQ  NM S IN+LWG++LYERSI+E+K+GLP W ECLEVAVEKFELAGASATDIA
Sbjct: 661 EEETVEQTANMSSQINLLWGSLLYERSIVEYKLGLPTWDECLEVAVEKFELAGASATDIA 720

Query: 721 VMIKNHCSSNNAHEGLGFKIDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVL 739
           VM+KNHCSS +A EG+GFKIDEIVQAWNEMY+A++   GVPSFRLEP+FRRR  K++ +L
Sbjct: 721 VMVKNHCSSESALEGMGFKIDEIVQAWNEMYDAKRWQMGVPSFRLEPMFRRRAPKLHDIL 775

BLAST of Spg022300 vs. ExPASy Swiss-Prot
Match: K7TQE3 (HSP-interacting protein OS=Zea mays OX=4577 GN=HIP PE=1 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 1.2e-159
Identity = 326/737 (44.23%), Postives = 467/737 (63.36%), Query Frame = 0

Query: 35  DKDHVIFITMSQVLKEEGNTLFQSRDLEGAMLKYEKALKLLPR-NHIDVSYLRSNMAACY 94
           D D  +F+ +S+ LKEEG  LF  RD EGA  KY+KA++LLP    ++ ++LR+++A CY
Sbjct: 16  DGDDAVFLELSRELKEEGTRLFNRRDFEGAAFKYDKAVQLLPAGRRVEAAHLRASIAHCY 75

Query: 95  MQMGLSEYPRAIHECNLALEVTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNI 154
           M+M  +E+  AIHECNLALE  P+YS+ALL+RA C+EAL R DLA  D+  VL  EP N 
Sbjct: 76  MRMSPAEFHHAIHECNLALEAVPRYSRALLRRAACFEALGRPDLAWGDIRTVLRWEPGNR 135

Query: 155 MALEISERLTEALETKG-SKDVDAEIKLPLDFVELPAAVLPQKRPKEKSRKKKNNQKTKE 214
            A +IS+R+  ALE KG S  +D    LP D  E+ +A       K + RKK  N++   
Sbjct: 136 AARQISDRVRTALEDKGISVALDV---LPEDENEIASA-------KGEERKKSRNKRFDS 195

Query: 215 TIDEKKADEIVEEKADEIVEEKAG----------DETVQEKADKIIVEEEKAEETGEE-- 274
               ++ +  +        E++AG           +  ++     + + E++ ETGE+  
Sbjct: 196 VAGGREGENGIALLESASTEKQAGPRQTNGTGNHQDHTEDSESNGLEKLEQSTETGEKDM 255

Query: 275 -------------KKAEDKLVVEEKIAKTQEEI-----PMKTVKLVFEEDIRWAQLPIGC 334
                        +  E K      +   Q+ I      MK VKLVF EDIR AQ+P  C
Sbjct: 256 GKKRGAHAAGKKPRCGESKQQKHSAVNHCQDNIGAKEEVMKDVKLVFGEDIRCAQMPANC 315

Query: 335 TLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAETSTESQGSVRFYIFEVS 394
           +L QLRE+++++FP+  A LIKY+D+E DLVTIT +EEL  A     SQ  +RFY+ EV+
Sbjct: 316 SLPQLREIVQNKFPSLKAFLIKYKDKEEDLVTITLSEELSWASNLAVSQVPIRFYVVEVN 375

Query: 395 PEKDPFYERLNNN-EVAKCKVEQSSVFENGHVLRAKEMKMSSCIDDWIIHFAQLFINHVG 454
             ++   + +      A  +  +  + +NG +    +++     DDW++ FAQ+F NHVG
Sbjct: 376 HVQELGVDGVRRRPSFATLERNRDIMLDNGTI--GHDVEHKHYADDWMVQFAQIFKNHVG 435

Query: 455 FESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHEMAALALFYWGNVIMSKA 514
           F S  YLDLHDLG++L+ EA+E+T+  EEAQ +F++A  KF EMAALALF  GNV MS+A
Sbjct: 436 FSSDAYLDLHDLGLRLHYEAMEDTIQREEAQEIFEVAESKFKEMAALALFNCGNVHMSRA 495

Query: 515 RKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEIKPDFYEGYLALGQQQFE 574
           R++    +   +  + +++  +YDW   EYA+AG +++ A++ K DF+EG +ALGQQ+FE
Sbjct: 496 RRRPCLAEDPLQEFILEKVNVSYDWACTEYAKAGAMFEEAVKTKSDFFEGLIALGQQKFE 555

Query: 575 QAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNMETGMKMWEEWEEQRTGEFSKSSNVK 634
           QAKLSW YA++  ++ +    TEVL+L+N+AEDNME GM MWE  E  R    SK S  K
Sbjct: 556 QAKLSWYYALACKINME----TEVLELFNHAEDNMEKGMDMWERMETLRLKGLSKPSKEK 615

Query: 635 TQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILWGTMLYERSILEFKMGLPVWHECLEV 694
             L+KM L+G +KDISA EA EQA ++RSHINILWGT+LYERS++EF +GLP W E L V
Sbjct: 616 VVLEKMVLEGFVKDISADEAFEQASSIRSHINILWGTILYERSVVEFNLGLPSWEESLTV 675

Query: 695 AVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNEMYEARKLLTGVPSFR 739
           A+EKF++ GAS  DI V++KNHC++    EGL FK++EIVQAW+EM++A+   +G   FR
Sbjct: 676 AMEKFKIGGASQADINVIVKNHCANETTQEGLSFKVEEIVQAWSEMHDAKNWRSGPLYFR 735

BLAST of Spg022300 vs. ExPASy Swiss-Prot
Match: F4K487 (Protein PHOX3 OS=Arabidopsis thaliana OX=3702 GN=PHOX3 PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 2.1e-148
Identity = 305/700 (43.57%), Postives = 460/700 (65.71%), Query Frame = 0

Query: 42  ITMSQVLKEEGNTLFQSRDLEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEY 101
           ++ +Q LKEEGN LFQ RD +GAM KY +A+K+LP++H++VS++R+N+A+CYMQ+   E+
Sbjct: 123 VSKAQGLKEEGNKLFQKRDYDGAMFKYGEAIKILPKDHVEVSHVRANVASCYMQLEPGEF 182

Query: 102 PRAIHECNLALEVTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISER 161
            +AIHEC+LAL VTP ++KALLKRARCYEAL++LDLALRDV  V  ++PKN MA EI E+
Sbjct: 183 AKAIHECDLALSVTPDHNKALLKRARCYEALNKLDLALRDVCMVSKLDPKNPMASEIVEK 242

Query: 162 LTEALETKGSKDVDAEIKLPLDFVE----LPAAVLPQKRPKEKSRKKKNNQKTKETIDEK 221
           L   LE+KG +  ++ I+LP D+VE     PAA+  +       + KK+NQ  +++  E 
Sbjct: 243 LKRTLESKGLRINNSVIELPPDYVEPVGASPAALWAKLGKVRVKKTKKSNQVEEKS--EG 302

Query: 222 KADEIVEEKADEIVEEKAGDETVQEKADKIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQ 281
           + +++  EK + ++ EK G E ++ K     V+ +++++  +  K ++K+++EE++    
Sbjct: 303 EGEDVEPEKKNNVLAEK-GKEKIKMK-----VKGKQSDKRSDTSKEQEKVIIEEELLVIG 362

Query: 282 EEIPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITT 341
            E   K VK V+ +DIR A+LPI CTL +LREV+ +RFP+  AV IKYRD+EGDLVTITT
Sbjct: 363 VEDVNKDVKFVYSDDIRLAELPINCTLFKLREVVHERFPSLRAVHIKYRDQEGDLVTITT 422

Query: 342 NEELRLAETSTESQGSVRFYIFEVSPEKDPFYERLNNNEVAKCKVEQSSVFENGHVLRAK 401
           +EELR++E S+ SQG++RFY+ EVSPE+DPF+ RL   E+ K K+   S        +AK
Sbjct: 423 DEELRMSEVSSRSQGTMRFYVVEVSPEQDPFFGRL--VEMKKLKITADS-------FKAK 482

Query: 402 EMKMSSC-IDDWIIHFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFD 461
                 C ++DW+I FA LF      +S   L+L +LGMKL +EA+EE VTS+ AQG FD
Sbjct: 483 VNGRGGCKVEDWMIEFAHLFKIQARIDSDRCLNLQELGMKLNSEAMEEVVTSDAAQGPFD 542

Query: 462 LAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGR 521
            AA++F E+AA +L   G V MS ARK++    G S   + +Q+K AY+  +KE+A A  
Sbjct: 543 RAAQQFQEVAARSLLNLGYVHMSGARKRLSLLQGVSGESVSEQVKTAYECAKKEHANAKE 602

Query: 522 IYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNM 581
            Y+ A++IKP+ +E +LALG QQFE+A+LSW Y + S++D KT P  +V++ Y +AE N+
Sbjct: 603 KYEEAMKIKPECFEVFLALGLQQFEEARLSWYYVLVSHLDLKTWPYADVVQFYQSAESNI 662

Query: 582 ETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILW 641
           +  M++ E  E  +  E S++        +  L    ++      A++A  ++S I+IL 
Sbjct: 663 KKSMEVLENLETGKESEPSQAGKTDCLTHEKDLGSSTQN----NPAKEAGRLKSWIDILL 722

Query: 642 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 701
             +LYERSI+E+K+  P W E LE A+EKFELAG    D+  +I     + N    + F 
Sbjct: 723 CAVLYERSIMEYKLDQPFWRESLEAAMEKFELAGTCKDDVVEIISEDYVAGNTLRDIRFH 782

Query: 702 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHV 737
           ++EI+Q ++E+YEA+    G+PS +LE + +RR   I+HV
Sbjct: 783 MEEIIQIFDEIYEAKHWTNGIPSDQLEEILKRRAENIFHV 801

BLAST of Spg022300 vs. ExPASy Swiss-Prot
Match: O48802 (Protein CLMP1 OS=Arabidopsis thaliana OX=3702 GN=CLMP1 PE=1 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 5.4e-120
Identity = 284/748 (37.97%), Postives = 419/748 (56.02%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREA-ISKHRQGGDGSPKS-----YDKDHVIFITMSQVLKEEGNT 60
           MGK GG+KKK  G     + ++     G   P +      D D  IF+  +  LKEEGN 
Sbjct: 1   MGKSGGRKKKSGGSNSNSSQVNSSETSGLSKPSTIVNGGVDFDASIFLKRAHELKEEGNK 60

Query: 61  LFQSRDLEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALEV 120
            FQ+RD  GA+ +YE  +KL+P++H D +   SN AAC MQM   +Y   I EC++AL+ 
Sbjct: 61  KFQARDYVGALEQYENGIKLIPKSHPDRAVFHSNRAACLMQMKPIDYESVISECSMALKS 120

Query: 121 TPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEAL---ETKGS 180
            P +++ALL+RAR +EA+ + DLA++DVN +L  +P +  A EIS+RL  AL   +   S
Sbjct: 121 QPGFTRALLRRARAFEAVGKFDLAVQDVNVLLGSDPNHKDAGEISKRLKTALGPHQDLQS 180

Query: 181 KDVDAEIKLPLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKADEIV 240
           +   A +          A + P    +   +K   +     ++      ++   +    V
Sbjct: 181 RPSPAALGASAALGGPIAGLGPCLPSRNVHKKGVTSPVGSVSLPNASNGKVERPQVVNPV 240

Query: 241 EEKAGDETVQEKADKIIVEE-------EKAEETGEEKKAEDKLVVEEKIAKTQEE-IPMK 300
            E  G  + + +A +++++         K EE G    A         + K QE+ I  +
Sbjct: 241 TENGGSVS-KGQASRVVLKPVSHSPKGSKVEELGSSSVA--------VVGKVQEKRIRWR 300

Query: 301 TVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRL 360
            +K V++ DIR  Q+P+ C   +LRE++  RFP+S AVLIKY+D +GDLVTIT+  EL+L
Sbjct: 301 PLKFVYDHDIRLGQMPVNCRFKELREIVSSRFPSSKAVLIKYKDNDGDLVTITSTAELKL 360

Query: 361 AETS-------------TESQGSVRFYIFEVSPEKDPFYERLNNNEVAKCKVEQSSVFEN 420
           AE++             ++S G +R ++ +VSPE++P        EV +  V +  +   
Sbjct: 361 AESAADCILTKEPDTDKSDSVGMLRLHVVDVSPEQEPMLLEEEEEEVEEKPVIEEVISSP 420

Query: 421 GHVLRAKEM------------KMSSC---------IDDWIIHFAQLFINHVGFESGPYLD 480
              L   E+            K SS          +DDW+  FA LF  HVG +   ++D
Sbjct: 421 TESLSETEINTEKTDKEVEKEKASSSEDPETKELEMDDWLFDFAHLFRTHVGIDPDAHID 480

Query: 481 LHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHEMAALALFYWGNVIMSKARKKVYFTD 540
           LH+LGM+L +EA+EETVTSE+AQ LFD A+ KF E+AALA F WGNV M  ARK++   +
Sbjct: 481 LHELGMELCSEALEETVTSEKAQPLFDKASAKFQEVAALAFFNWGNVHMCAARKRIPLDE 540

Query: 541 GASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLY 600
            A K  +  Q++ AY+WV++ Y  A   Y+ A+ IKPDFYEG LALGQQQFE AKL W Y
Sbjct: 541 SAGKEVVAAQLQTAYEWVKERYTLAKEKYEQALSIKPDFYEGLLALGQQQFEMAKLHWSY 600

Query: 601 AVSSNVDSKTLPCTEVLKLYNNAEDNMETGMKMWEEWEEQRTGEF-----SKSSNVKTQL 660
            ++  +D      +E L L+++AE  M+   +MWE+ EEQR  +      +K   V  + 
Sbjct: 601 LLAQKIDISGWDPSETLNLFDSAEAKMKDATEMWEKLEEQRMDDLKNPNSNKKEEVSKRR 660

Query: 661 QKMGLDG---LIKDISAAEAAEQAKNMRSHINILWGTMLYERSILEFKMGLPVWHECLEV 690
           +K G DG   + + I+A EAAEQA  MRS I++ WG ML+ERS +E K+G   W++ L+ 
Sbjct: 661 KKQGGDGNEEVSETITAEEAAEQATAMRSQIHLFWGNMLFERSQVECKIGKDGWNKNLDS 720

BLAST of Spg022300 vs. ExPASy TrEMBL
Match: A0A6J1HVI3 (protein PHOX1-like OS=Cucurbita maxima OX=3661 GN=LOC111468214 PE=4 SV=1)

HSP 1 Score: 1253.4 bits (3242), Expect = 0.0e+00
Identity = 654/764 (85.60%), Postives = 687/764 (89.92%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNTLFQSRD 60
           MGKQ GKKKKQIGDKFRE I+KHRQ GDGSP + DKDH IFITMS  LKEEGN LFQ+RD
Sbjct: 1   MGKQSGKKKKQIGDKFREVIAKHRQSGDGSPSNNDKDHAIFITMSLALKEEGNRLFQARD 60

Query: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALEVTPKYSK 120
           LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMG+SEYPRAIHECNLALEVTPKYSK
Sbjct: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGISEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSKDVDAEIKL 180
           ALLKRARCYEALHRLDLALRDVN VLNMEP NIMALEISERLT+ALETKGSK+ DAEIKL
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNTVLNMEPNNIMALEISERLTKALETKGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKA------------D 240
           PLDFVELPA VLPQK  KEK+RKKKNNQKTKETIDEKKADEIVEEK             +
Sbjct: 181 PLDFVELPAEVLPQKGSKEKNRKKKNNQKTKETIDEKKADEIVEEKVGNETVEVEKAGNE 240

Query: 241 EIVEEKAGDETV-----------QEKADKIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQ 300
            + EEKAG+ETV           +EKA    VEEEKA+ET EEKKAEDKLVVEEKIA+  
Sbjct: 241 TVEEEKAGNETVEEETAGNGTVAEEKAGNGTVEEEKAKETVEEKKAEDKLVVEEKIARAL 300

Query: 301 EEIPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITT 360
           EE PMKTVKLVF EDIRWAQ+P+ CTLLQLRE IRDRFPT  AVLIKYRDEEGDLVTIT 
Sbjct: 301 EETPMKTVKLVFGEDIRWAQVPVDCTLLQLREAIRDRFPTCPAVLIKYRDEEGDLVTITA 360

Query: 361 NEELRLAETSTESQGSVRFYIFEVSPEKDPFYERL-NNNEVAKCKVEQSSVFENGHVLRA 420
           NEELR+AET+  SQ SVRFYIFEV PE+DPFYE+   ++EVAKCKVEQSS+FENGH+LRA
Sbjct: 361 NEELRMAETAKASQASVRFYIFEVKPEQDPFYEKFKKDDEVAKCKVEQSSIFENGHLLRA 420

Query: 421 KEMKMSSCIDDWIIHFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFD 480
           KE+K SSCIDDWII FAQLFINHVGFESGP LDLHDLGMKLY+EAVEETVTSEEAQGLF 
Sbjct: 421 KEIKTSSCIDDWIIQFAQLFINHVGFESGPCLDLHDLGMKLYSEAVEETVTSEEAQGLFK 480

Query: 481 LAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGR 540
           LAAEKFHEM ALALFYWGNVIMS ARKKVYFTDGASK PLF+QIK+AYDWVE EY EAGR
Sbjct: 481 LAAEKFHEMTALALFYWGNVIMSSARKKVYFTDGASKGPLFEQIKSAYDWVENEYVEAGR 540

Query: 541 IYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNM 600
            YQMA+EIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVD KTLPCTEVLKLYN AE++M
Sbjct: 541 KYQMAVEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDPKTLPCTEVLKLYNYAEESM 600

Query: 601 ETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILW 660
           ETGMKMWEEWEEQRT EFSK+S VKTQLQKMGLDGLIKDISA EAAEQAKNMRSHIN+LW
Sbjct: 601 ETGMKMWEEWEEQRTSEFSKTSTVKTQLQKMGLDGLIKDISADEAAEQAKNMRSHINLLW 660

Query: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720
           GTMLYERSILEFKMG+PVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK
Sbjct: 661 GTMLYERSILEFKMGIPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720

Query: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 741
           IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA
Sbjct: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 764

BLAST of Spg022300 vs. ExPASy TrEMBL
Match: A0A6J1EXN1 (protein PHOX1-like OS=Cucurbita moschata OX=3662 GN=LOC111439405 PE=4 SV=1)

HSP 1 Score: 1248.4 bits (3229), Expect = 0.0e+00
Identity = 652/764 (85.34%), Postives = 686/764 (89.79%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNTLFQSRD 60
           MGKQ GKKKKQIGDKFRE I+KHRQ GDGSP + DKDH IFITMS  LKEEGN LFQ+RD
Sbjct: 1   MGKQSGKKKKQIGDKFREVIAKHRQSGDGSPSNNDKDHAIFITMSLALKEEGNRLFQARD 60

Query: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALEVTPKYSK 120
           LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMG+SEYPRAIHECNLALEVTPKYSK
Sbjct: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGISEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSKDVDAEIKL 180
           ALLKRARCYEALHRLDLALRDVN VLNMEP NIMALEISERLT+ALETKGSK+ DAEIKL
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNTVLNMEPNNIMALEISERLTKALETKGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKA------------D 240
           PLDFVELPA VLPQK  KEK+RKKKNNQKTKETI EKKADEIVEEK             +
Sbjct: 181 PLDFVELPAEVLPQKGSKEKNRKKKNNQKTKETIIEKKADEIVEEKVGNETVEVEKAGNE 240

Query: 241 EIVEEKAGDETV-----------QEKADKIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQ 300
            + EEKAG+ETV           +EKA    VEEEKA+E+ EEKKAEDKLVVEEKIA+  
Sbjct: 241 TVEEEKAGNETVEEETAGNGTVAEEKAGNGTVEEEKAKESVEEKKAEDKLVVEEKIARAL 300

Query: 301 EEIPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITT 360
           EE PMKTVKLVF EDIRWAQ+P+ CTLLQLRE IRDRFPT  AVLIKYRDEEGDLVTIT 
Sbjct: 301 EETPMKTVKLVFGEDIRWAQVPVDCTLLQLREAIRDRFPTCPAVLIKYRDEEGDLVTITA 360

Query: 361 NEELRLAETSTESQGSVRFYIFEVSPEKDPFYERL-NNNEVAKCKVEQSSVFENGHVLRA 420
           NEELR+AET+  SQ SVRFYIFEV PE+DPFYE+   ++EVAKCKVEQSS+FENGH+LRA
Sbjct: 361 NEELRMAETAKASQASVRFYIFEVKPEQDPFYEKFKKDDEVAKCKVEQSSIFENGHLLRA 420

Query: 421 KEMKMSSCIDDWIIHFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFD 480
           KE+K SSCIDDWII FAQLFINHVGFESGP LDLHDLGMKLY+EAVEETVTSEEAQGLF 
Sbjct: 421 KEIKTSSCIDDWIIQFAQLFINHVGFESGPCLDLHDLGMKLYSEAVEETVTSEEAQGLFK 480

Query: 481 LAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGR 540
           LAAEKFHEM ALALFYWGNVIMS ARKKVYFTDGASK PLF+Q+K+AYDWVE EY EAGR
Sbjct: 481 LAAEKFHEMTALALFYWGNVIMSSARKKVYFTDGASKEPLFEQVKSAYDWVENEYVEAGR 540

Query: 541 IYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNM 600
            YQMA+EIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVD KTLPCTEVLKLYN AE++M
Sbjct: 541 KYQMAVEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDPKTLPCTEVLKLYNYAEESM 600

Query: 601 ETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILW 660
           ETGMKMWEEWEEQRT EFSK+S VKTQLQKMGLDGLIKDISA EAAEQAKNMRSHIN+LW
Sbjct: 601 ETGMKMWEEWEEQRTSEFSKTSTVKTQLQKMGLDGLIKDISADEAAEQAKNMRSHINLLW 660

Query: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720
           GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK
Sbjct: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720

Query: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 741
           IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA
Sbjct: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 764

BLAST of Spg022300 vs. ExPASy TrEMBL
Match: A0A6J1JHE2 (protein PHOX1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485121 PE=4 SV=1)

HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 642/742 (86.52%), Postives = 687/742 (92.59%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNTLFQSRD 60
           MGKQ GKKKKQIGDK+REAISK RQGG GS  SYDKDHVIFITMSQ LKEEGN LFQSRD
Sbjct: 1   MGKQTGKKKKQIGDKYREAISKRRQGGVGS-SSYDKDHVIFITMSQALKEEGNKLFQSRD 60

Query: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALEVTPKYSK 120
           +EGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYP+AIHECNLALEVTPKYSK
Sbjct: 61  VEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPQAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSKDVDAEIKL 180
           ALLKRARCYEALHRLDLALRDVNAVLN+EP+NIMALEISERLT+AL  +GSK+ DAEIKL
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNAVLNLEPRNIMALEISERLTKALMMQGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKADE-IVEEKAGDET 240
           PLDFVELP+++LPQ+RPKEK+RK KNNQKTKETIDEKKA E VEEKADE IVEE+  DET
Sbjct: 181 PLDFVELPSSLLPQERPKEKNRKMKNNQKTKETIDEKKAHETVEEKADEIIVEEEKADET 240

Query: 241 VQE-KADKIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQEEIPMKTVKLVFEEDIRWAQL 300
           V+E KAD+IIVEEEKA+ET EEKK EDKLVVEEKI +TQEE PMK+VKLVF +DIRWAQ+
Sbjct: 241 VEEKKADEIIVEEEKADETVEEKKPEDKLVVEEKINRTQEETPMKSVKLVFGDDIRWAQV 300

Query: 301 PIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAETSTESQGSVRFYI 360
           PI CTLLQLREVI+DRFPT TAVLIKYRDEEGDLVTIT +EELRLAETS ESQGSVRFYI
Sbjct: 301 PIDCTLLQLREVIQDRFPTCTAVLIKYRDEEGDLVTITADEELRLAETSKESQGSVRFYI 360

Query: 361 FEVSPEKDPFYERLNNNEVAKCKVEQSSVFENGHVLRAKEMKMSSCIDDWIIHFAQLFIN 420
           FEV+PE+DPFY R  N+E +KCKVE     EN +VLRAKEMK+SSCIDDW+IHFAQLFIN
Sbjct: 361 FEVNPEQDPFYGRFKNDEDSKCKVE-----ENSYVLRAKEMKISSCIDDWLIHFAQLFIN 420

Query: 421 HVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHEMAALALFYWGNVIM 480
           HVGFESGPYLDLHDLGMKLY+EAVEETVTSEEAQGLF+ AAEKFHEMAALALFYWGNVIM
Sbjct: 421 HVGFESGPYLDLHDLGMKLYSEAVEETVTSEEAQGLFESAAEKFHEMAALALFYWGNVIM 480

Query: 481 SKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEIKPDFYEGYLALGQQ 540
           S+ARKKVYFTDG SK  L +QIKAAYDWVEK Y EAG  YQMA++IKPDFYEGYLALGQQ
Sbjct: 481 SRARKKVYFTDGVSKVSLSEQIKAAYDWVEKAYVEAGNKYQMAVKIKPDFYEGYLALGQQ 540

Query: 541 QFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNMETGMKMWEEWEEQRTGEFSKSS 600
           QFEQAKLSW YAVSSNVD KT PCT+V+ LYNNAEDNMETGM+MWEEWE+Q T E SKSS
Sbjct: 541 QFEQAKLSWHYAVSSNVDPKTWPCTKVIHLYNNAEDNMETGMRMWEEWEKQHTAELSKSS 600

Query: 601 NVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILWGTMLYERSILEFKMGLPVWHEC 660
           +V+T LQKMGLDGLIKDISA EAAEQAKNMRSHIN+LWGTMLYERSILEFKMGLP WHEC
Sbjct: 601 DVETPLQKMGLDGLIKDISADEAAEQAKNMRSHINLLWGTMLYERSILEFKMGLPSWHEC 660

Query: 661 LEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNEMYEARKLLTGVP 720
           LEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKI+EIVQAWNEMYEARKLLTGVP
Sbjct: 661 LEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIEEIVQAWNEMYEARKLLTGVP 720

Query: 721 SFRLEPLFRRRVSKIYHVLEEA 741
           SFRLEPLFRRRVSKIYHVLE+A
Sbjct: 721 SFRLEPLFRRRVSKIYHVLEQA 736

BLAST of Spg022300 vs. ExPASy TrEMBL
Match: A0A6J1JJD6 (protein PHOX1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485121 PE=4 SV=1)

HSP 1 Score: 1224.9 bits (3168), Expect = 0.0e+00
Identity = 642/761 (84.36%), Postives = 687/761 (90.28%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNTLFQSRD 60
           MGKQ GKKKKQIGDK+REAISK RQGG GS  SYDKDHVIFITMSQ LKEEGN LFQSRD
Sbjct: 1   MGKQTGKKKKQIGDKYREAISKRRQGGVGS-SSYDKDHVIFITMSQALKEEGNKLFQSRD 60

Query: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALEVTPKYSK 120
           +EGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYP+AIHECNLALEVTPKYSK
Sbjct: 61  VEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPQAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSKDVDAEIKL 180
           ALLKRARCYEALHRLDLALRDVNAVLN+EP+NIMALEISERLT+AL  +GSK+ DAEIKL
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNAVLNLEPRNIMALEISERLTKALMMQGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKADE----------- 240
           PLDFVELP+++LPQ+RPKEK+RK KNNQKTKETIDEKKA E VEEKADE           
Sbjct: 181 PLDFVELPSSLLPQERPKEKNRKMKNNQKTKETIDEKKAHETVEEKADEIIVEEEKADET 240

Query: 241 ---------IVEEKAGDETVQE-KADKIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQEE 300
                    IVEE+  DETV+E KAD+IIVEEEKA+ET EEKK EDKLVVEEKI +TQEE
Sbjct: 241 VEEKKADEIIVEEEKADETVEEKKADEIIVEEEKADETVEEKKPEDKLVVEEKINRTQEE 300

Query: 301 IPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNE 360
            PMK+VKLVF +DIRWAQ+PI CTLLQLREVI+DRFPT TAVLIKYRDEEGDLVTIT +E
Sbjct: 301 TPMKSVKLVFGDDIRWAQVPIDCTLLQLREVIQDRFPTCTAVLIKYRDEEGDLVTITADE 360

Query: 361 ELRLAETSTESQGSVRFYIFEVSPEKDPFYERLNNNEVAKCKVEQSSVFENGHVLRAKEM 420
           ELRLAETS ESQGSVRFYIFEV+PE+DPFY R  N+E +KCKVE     EN +VLRAKEM
Sbjct: 361 ELRLAETSKESQGSVRFYIFEVNPEQDPFYGRFKNDEDSKCKVE-----ENSYVLRAKEM 420

Query: 421 KMSSCIDDWIIHFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAA 480
           K+SSCIDDW+IHFAQLFINHVGFESGPYLDLHDLGMKLY+EAVEETVTSEEAQGLF+ AA
Sbjct: 421 KISSCIDDWLIHFAQLFINHVGFESGPYLDLHDLGMKLYSEAVEETVTSEEAQGLFESAA 480

Query: 481 EKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQ 540
           EKFHEMAALALFYWGNVIMS+ARKKVYFTDG SK  L +QIKAAYDWVEK Y EAG  YQ
Sbjct: 481 EKFHEMAALALFYWGNVIMSRARKKVYFTDGVSKVSLSEQIKAAYDWVEKAYVEAGNKYQ 540

Query: 541 MAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNMETG 600
           MA++IKPDFYEGYLALGQQQFEQAKLSW YAVSSNVD KT PCT+V+ LYNNAEDNMETG
Sbjct: 541 MAVKIKPDFYEGYLALGQQQFEQAKLSWHYAVSSNVDPKTWPCTKVIHLYNNAEDNMETG 600

Query: 601 MKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILWGTM 660
           M+MWEEWE+Q T E SKSS+V+T LQKMGLDGLIKDISA EAAEQAKNMRSHIN+LWGTM
Sbjct: 601 MRMWEEWEKQHTAELSKSSDVETPLQKMGLDGLIKDISADEAAEQAKNMRSHINLLWGTM 660

Query: 661 LYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDE 720
           LYERSILEFKMGLP WHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKI+E
Sbjct: 661 LYERSILEFKMGLPSWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIEE 720

Query: 721 IVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 741
           IVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLE+A
Sbjct: 721 IVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEQA 755

BLAST of Spg022300 vs. ExPASy TrEMBL
Match: A0A6J1BZI4 (protein PHOX1-like OS=Momordica charantia OX=3673 GN=LOC111007136 PE=4 SV=1)

HSP 1 Score: 1224.9 bits (3168), Expect = 0.0e+00
Identity = 639/754 (84.75%), Postives = 684/754 (90.72%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNTLFQSRD 60
           MGKQ GKKKKQIGDKFREAISK RQGGD S +SYDKD+ IFI MSQ LKEEGN LFQSRD
Sbjct: 1   MGKQSGKKKKQIGDKFREAISKQRQGGDSSLRSYDKDNSIFIIMSQALKEEGNKLFQSRD 60

Query: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALEVTPKYSK 120
           LEGAM KYEKALKLLP+NHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALEVTPKYSK
Sbjct: 61  LEGAMFKYEKALKLLPKNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSKDVDAEIKL 180
           ALLKRARCYEALHRLDLALRDVN VLNMEPKNIMALEIS+R+T+ALE K S + DAEI+L
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNTVLNMEPKNIMALEISDRVTKALEMKKSNEDDAEIQL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIV-EEKADEI-VEEKAGDE 240
           PLDFVELP+ VLPQK  KEK+RKKK NQKTKETI EKK DE V EEKADEI V EK  DE
Sbjct: 181 PLDFVELPSPVLPQKIHKEKNRKKK-NQKTKETIGEKKTDETVEEEKADEIKVREKETDE 240

Query: 241 TV------------QEKADKIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQEEIPMKTVK 300
            +            ++KAD+II  E++A+ET EEKKAEDKLVVEEKI +T EE PMK VK
Sbjct: 241 IIVRDKEADEITVREKKADEIIAGEKRADETVEEKKAEDKLVVEEKIRRTHEETPMKAVK 300

Query: 301 LVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAET 360
           LVF EDIRWAQLPI CTLL+LREVIRDRFPT  AVLIKYRDEEGDLVTITTNEELRLAE 
Sbjct: 301 LVFGEDIRWAQLPIDCTLLRLREVIRDRFPTCRAVLIKYRDEEGDLVTITTNEELRLAEA 360

Query: 361 STESQGSVRFYIFEVSPEKDPFYERLNNNEVAKCKVEQSSVFENGHVLRAKEMKMSSCID 420
           +TESQGSVRFYIFEV+PE+DPFY R  N+EV KCKVEQ+S+FENGHVLR KE+ MSSCID
Sbjct: 361 TTESQGSVRFYIFEVNPEQDPFYGRFKNDEVEKCKVEQNSMFENGHVLRGKEIMMSSCID 420

Query: 421 DWIIHFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHEMA 480
           DWI+ FAQLFINHVGFESG YLDL +LGMKLY+EA+EETVTSEEAQGLF++AAEKFHEMA
Sbjct: 421 DWIVQFAQLFINHVGFESGSYLDLQELGMKLYSEAMEETVTSEEAQGLFEIAAEKFHEMA 480

Query: 481 ALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEIKP 540
           ALA+FYWGNVIMS+ARKKVYFTD ASKAP+F+QIKAAYDWV+KEY EAG+ YQ+A+EIKP
Sbjct: 481 ALAMFYWGNVIMSRARKKVYFTDSASKAPVFEQIKAAYDWVKKEYVEAGQKYQIAVEIKP 540

Query: 541 DFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNMETGMKMWEEW 600
           DFYEGYLALGQQQFEQAKLSW YAVSSNVD KT PCTEVL+LYNNAEDNMETGMK+WEEW
Sbjct: 541 DFYEGYLALGQQQFEQAKLSWQYAVSSNVDLKTWPCTEVLQLYNNAEDNMETGMKIWEEW 600

Query: 601 EEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILWGTMLYERSIL 660
           EEQR  E SKSSNVKTQLQKMGLDGLIKDISA EAAEQAKNMRSHIN+LWGTMLYERSIL
Sbjct: 601 EEQRASELSKSSNVKTQLQKMGLDGLIKDISADEAAEQAKNMRSHINLLWGTMLYERSIL 660

Query: 661 EFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNE 720
           EFK+GLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNE
Sbjct: 661 EFKLGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNE 720

Query: 721 MYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 741
           MYEARKLLTGVPSFRLEPLFRRRVSKIYH+LEEA
Sbjct: 721 MYEARKLLTGVPSFRLEPLFRRRVSKIYHILEEA 753

BLAST of Spg022300 vs. TAIR 10
Match: AT2G25290.1 (Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide repeat (TPR)-containing protein )

HSP 1 Score: 716.5 bits (1848), Expect = 2.3e-206
Identity = 406/751 (54.06%), Postives = 526/751 (70.04%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGD-------GSPKSYDKDHVIFITMSQVLKEEGN 60
           MGK  GKKK     +     S    GG         + KS+D D  IFI  +  LKEEGN
Sbjct: 1   MGKPTGKKKNNNYTEMPPTESSTTGGGKTGKSFDRSATKSFDDDMTIFINRALELKEEGN 60

Query: 61  TLFQSRDLEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALE 120
            LFQ RD EGAM +Y+KA+KLLPR+H DV+YLR++MA+CYMQMGL EYP AI+ECNLALE
Sbjct: 61  KLFQKRDYEGAMFRYDKAVKLLPRDHGDVAYLRTSMASCYMQMGLGEYPNAINECNLALE 120

Query: 121 VTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSKD 180
            +P++SKALLKRARCYEAL++LD A RD   VLNMEP+N+ A EI ER+ + L  KG  D
Sbjct: 121 ASPRFSKALLKRARCYEALNKLDFAFRDSRVVLNMEPENVSANEIFERVKKVLVGKGI-D 180

Query: 181 VDAEIKLPLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKADEIVEE 240
           VD E++  L  V+   A   +K  KE+ RKKK     K+++         E K+ E V E
Sbjct: 181 VD-EMEKNLVNVQPVGAARLRKIVKERLRKKK-----KKSMTMTNGGNDGERKSVEAVVE 240

Query: 241 KAGDETVQEKADKIIVEEEKAEETGEEKKAEDKLVVEEK---IAKTQEEIPM-KTVKLVF 300
            A  +  +E      V+  +  +  EEKK EDK+ V +K    ++ +E+  + +TVKLV 
Sbjct: 241 DAKVDNGEE------VDSGRKGKAIEEKKLEDKVAVMDKEVIASEIKEDATVTRTVKLVH 300

Query: 301 EEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAETSTE 360
            +DIRWAQLP+  +++ +R+VI+DRFP     LIKYRD EGDLVTITT +ELRLA ++ E
Sbjct: 301 GDDIRWAQLPLDSSVVLVRDVIKDRFPALKGFLIKYRDSEGDLVTITTTDELRLAASTRE 360

Query: 361 SQGSVRFYIFEVSPEKDPFYERLNNNE-VAKCKVEQSSVFENGHVLRAKEM-KMSSCIDD 420
             GS R YI EVSP ++P Y+ ++N+E   K     SSV +NG V    E  K S+ ++ 
Sbjct: 361 KLGSFRLYIAEVSPNQEPTYDVIDNDESTDKFAKGSSSVADNGSVGDFVESEKASTSLEH 420

Query: 421 WIIHFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHEMAA 480
           WI  FAQLF NHVGF+S  YL+LH+LGMKLYTEA+E+ VT E+AQ LFD+AA+KF EMAA
Sbjct: 421 WIFQFAQLFKNHVGFDSDSYLELHNLGMKLYTEAMEDIVTGEDAQELFDIAADKFQEMAA 480

Query: 481 LALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEIKPD 540
           LA+F WGNV MSKAR+++YF +  S+  + ++++A ++W + EY +A   Y+ A++IK D
Sbjct: 481 LAMFNWGNVHMSKARRQIYFPEDGSRETILEKVEAGFEWAKNEYNKAAEKYEGAVKIKSD 540

Query: 541 FYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNMETGMKMWEEWE 600
           FYE  LALGQQQFEQAKL W +A+S  VD ++    +VLKLYN AE++ME GM++WEE E
Sbjct: 541 FYEALLALGQQQFEQAKLCWYHALSGEVDIESDASQDVLKLYNKAEESMEKGMQIWEEME 600

Query: 601 EQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILWGTMLYERSILE 660
           E+R    S     K  LQK+GLDG+  + S  E+AEQ  NM S IN+LWG++LYERSI+E
Sbjct: 601 ERRLNGISNFDKHKELLQKLGLDGIFSEASDEESAEQTANMSSQINLLWGSLLYERSIVE 660

Query: 661 FKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNEM 720
           +K+GLP W ECLEVAVEKFELAGASATDIAVM+KNHCSS+NA EG+GFKIDEIVQAWNEM
Sbjct: 661 YKLGLPTWDECLEVAVEKFELAGASATDIAVMVKNHCSSDNALEGMGFKIDEIVQAWNEM 720

Query: 721 YEARKLLTGVPSFRLEPLFRRRVSKIYHVLE 739
           Y+A++   GVPSFRLEPLFRRR  K++ +LE
Sbjct: 721 YDAKRWQIGVPSFRLEPLFRRRSPKLHDILE 738

BLAST of Spg022300 vs. TAIR 10
Match: AT2G25290.2 (Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide repeat (TPR)-containing protein )

HSP 1 Score: 716.5 bits (1848), Expect = 2.3e-206
Identity = 406/751 (54.06%), Postives = 526/751 (70.04%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGD-------GSPKSYDKDHVIFITMSQVLKEEGN 60
           MGK  GKKK     +     S    GG         + KS+D D  IFI  +  LKEEGN
Sbjct: 1   MGKPTGKKKNNNYTEMPPTESSTTGGGKTGKSFDRSATKSFDDDMTIFINRALELKEEGN 60

Query: 61  TLFQSRDLEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALE 120
            LFQ RD EGAM +Y+KA+KLLPR+H DV+YLR++MA+CYMQMGL EYP AI+ECNLALE
Sbjct: 61  KLFQKRDYEGAMFRYDKAVKLLPRDHGDVAYLRTSMASCYMQMGLGEYPNAINECNLALE 120

Query: 121 VTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSKD 180
            +P++SKALLKRARCYEAL++LD A RD   VLNMEP+N+ A EI ER+ + L  KG  D
Sbjct: 121 ASPRFSKALLKRARCYEALNKLDFAFRDSRVVLNMEPENVSANEIFERVKKVLVGKGI-D 180

Query: 181 VDAEIKLPLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKADEIVEE 240
           VD E++  L  V+   A   +K  KE+ RKKK     K+++         E K+ E V E
Sbjct: 181 VD-EMEKNLVNVQPVGAARLRKIVKERLRKKK-----KKSMTMTNGGNDGERKSVEAVVE 240

Query: 241 KAGDETVQEKADKIIVEEEKAEETGEEKKAEDKLVVEEK---IAKTQEEIPM-KTVKLVF 300
            A  +  +E      V+  +  +  EEKK EDK+ V +K    ++ +E+  + +TVKLV 
Sbjct: 241 DAKVDNGEE------VDSGRKGKAIEEKKLEDKVAVMDKEVIASEIKEDATVTRTVKLVH 300

Query: 301 EEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAETSTE 360
            +DIRWAQLP+  +++ +R+VI+DRFP     LIKYRD EGDLVTITT +ELRLA ++ E
Sbjct: 301 GDDIRWAQLPLDSSVVLVRDVIKDRFPALKGFLIKYRDSEGDLVTITTTDELRLAASTRE 360

Query: 361 SQGSVRFYIFEVSPEKDPFYERLNNNE-VAKCKVEQSSVFENGHVLRAKEM-KMSSCIDD 420
             GS R YI EVSP ++P Y+ ++N+E   K     SSV +NG V    E  K S+ ++ 
Sbjct: 361 KLGSFRLYIAEVSPNQEPTYDVIDNDESTDKFAKGSSSVADNGSVGDFVESEKASTSLEH 420

Query: 421 WIIHFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHEMAA 480
           WI  FAQLF NHVGF+S  YL+LH+LGMKLYTEA+E+ VT E+AQ LFD+AA+KF EMAA
Sbjct: 421 WIFQFAQLFKNHVGFDSDSYLELHNLGMKLYTEAMEDIVTGEDAQELFDIAADKFQEMAA 480

Query: 481 LALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEIKPD 540
           LA+F WGNV MSKAR+++YF +  S+  + ++++A ++W + EY +A   Y+ A++IK D
Sbjct: 481 LAMFNWGNVHMSKARRQIYFPEDGSRETILEKVEAGFEWAKNEYNKAAEKYEGAVKIKSD 540

Query: 541 FYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNMETGMKMWEEWE 600
           FYE  LALGQQQFEQAKL W +A+S  VD ++    +VLKLYN AE++ME GM++WEE E
Sbjct: 541 FYEALLALGQQQFEQAKLCWYHALSGEVDIESDASQDVLKLYNKAEESMEKGMQIWEEME 600

Query: 601 EQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILWGTMLYERSILE 660
           E+R    S     K  LQK+GLDG+  + S  E+AEQ  NM S IN+LWG++LYERSI+E
Sbjct: 601 ERRLNGISNFDKHKELLQKLGLDGIFSEASDEESAEQTANMSSQINLLWGSLLYERSIVE 660

Query: 661 FKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNEM 720
           +K+GLP W ECLEVAVEKFELAGASATDIAVM+KNHCSS+NA EG+GFKIDEIVQAWNEM
Sbjct: 661 YKLGLPTWDECLEVAVEKFELAGASATDIAVMVKNHCSSDNALEGMGFKIDEIVQAWNEM 720

Query: 721 YEARKLLTGVPSFRLEPLFRRRVSKIYHVLE 739
           Y+A++   GVPSFRLEPLFRRR  K++ +LE
Sbjct: 721 YDAKRWQIGVPSFRLEPLFRRRSPKLHDILE 738

BLAST of Spg022300 vs. TAIR 10
Match: AT2G25290.3 (Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide repeat (TPR)-containing protein )

HSP 1 Score: 716.5 bits (1848), Expect = 2.3e-206
Identity = 406/751 (54.06%), Postives = 526/751 (70.04%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGD-------GSPKSYDKDHVIFITMSQVLKEEGN 60
           MGK  GKKK     +     S    GG         + KS+D D  IFI  +  LKEEGN
Sbjct: 1   MGKPTGKKKNNNYTEMPPTESSTTGGGKTGKSFDRSATKSFDDDMTIFINRALELKEEGN 60

Query: 61  TLFQSRDLEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALE 120
            LFQ RD EGAM +Y+KA+KLLPR+H DV+YLR++MA+CYMQMGL EYP AI+ECNLALE
Sbjct: 61  KLFQKRDYEGAMFRYDKAVKLLPRDHGDVAYLRTSMASCYMQMGLGEYPNAINECNLALE 120

Query: 121 VTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSKD 180
            +P++SKALLKRARCYEAL++LD A RD   VLNMEP+N+ A EI ER+ + L  KG  D
Sbjct: 121 ASPRFSKALLKRARCYEALNKLDFAFRDSRVVLNMEPENVSANEIFERVKKVLVGKGI-D 180

Query: 181 VDAEIKLPLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKADEIVEE 240
           VD E++  L  V+   A   +K  KE+ RKKK     K+++         E K+ E V E
Sbjct: 181 VD-EMEKNLVNVQPVGAARLRKIVKERLRKKK-----KKSMTMTNGGNDGERKSVEAVVE 240

Query: 241 KAGDETVQEKADKIIVEEEKAEETGEEKKAEDKLVVEEK---IAKTQEEIPM-KTVKLVF 300
            A  +  +E      V+  +  +  EEKK EDK+ V +K    ++ +E+  + +TVKLV 
Sbjct: 241 DAKVDNGEE------VDSGRKGKAIEEKKLEDKVAVMDKEVIASEIKEDATVTRTVKLVH 300

Query: 301 EEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAETSTE 360
            +DIRWAQLP+  +++ +R+VI+DRFP     LIKYRD EGDLVTITT +ELRLA ++ E
Sbjct: 301 GDDIRWAQLPLDSSVVLVRDVIKDRFPALKGFLIKYRDSEGDLVTITTTDELRLAASTRE 360

Query: 361 SQGSVRFYIFEVSPEKDPFYERLNNNE-VAKCKVEQSSVFENGHVLRAKEM-KMSSCIDD 420
             GS R YI EVSP ++P Y+ ++N+E   K     SSV +NG V    E  K S+ ++ 
Sbjct: 361 KLGSFRLYIAEVSPNQEPTYDVIDNDESTDKFAKGSSSVADNGSVGDFVESEKASTSLEH 420

Query: 421 WIIHFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHEMAA 480
           WI  FAQLF NHVGF+S  YL+LH+LGMKLYTEA+E+ VT E+AQ LFD+AA+KF EMAA
Sbjct: 421 WIFQFAQLFKNHVGFDSDSYLELHNLGMKLYTEAMEDIVTGEDAQELFDIAADKFQEMAA 480

Query: 481 LALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEIKPD 540
           LA+F WGNV MSKAR+++YF +  S+  + ++++A ++W + EY +A   Y+ A++IK D
Sbjct: 481 LAMFNWGNVHMSKARRQIYFPEDGSRETILEKVEAGFEWAKNEYNKAAEKYEGAVKIKSD 540

Query: 541 FYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNMETGMKMWEEWE 600
           FYE  LALGQQQFEQAKL W +A+S  VD ++    +VLKLYN AE++ME GM++WEE E
Sbjct: 541 FYEALLALGQQQFEQAKLCWYHALSGEVDIESDASQDVLKLYNKAEESMEKGMQIWEEME 600

Query: 601 EQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILWGTMLYERSILE 660
           E+R    S     K  LQK+GLDG+  + S  E+AEQ  NM S IN+LWG++LYERSI+E
Sbjct: 601 ERRLNGISNFDKHKELLQKLGLDGIFSEASDEESAEQTANMSSQINLLWGSLLYERSIVE 660

Query: 661 FKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNEM 720
           +K+GLP W ECLEVAVEKFELAGASATDIAVM+KNHCSS+NA EG+GFKIDEIVQAWNEM
Sbjct: 661 YKLGLPTWDECLEVAVEKFELAGASATDIAVMVKNHCSSDNALEGMGFKIDEIVQAWNEM 720

Query: 721 YEARKLLTGVPSFRLEPLFRRRVSKIYHVLE 739
           Y+A++   GVPSFRLEPLFRRR  K++ +LE
Sbjct: 721 YDAKRWQIGVPSFRLEPLFRRRSPKLHDILE 738

BLAST of Spg022300 vs. TAIR 10
Match: AT4G32070.1 (Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide repeat (TPR)-containing protein )

HSP 1 Score: 688.3 bits (1775), Expect = 6.6e-198
Identity = 404/810 (49.88%), Postives = 528/810 (65.19%), Query Frame = 0

Query: 1   MGKQGGKKKKQIGDKFREAISKHRQGGDGSPKSY--------DKDHVIFITMSQVLKEEG 60
           MGK   KKK    +  ++A      GG  S K+Y        D+D  IFI+ +  LKEEG
Sbjct: 1   MGKPTAKKKNP--ETPKDASGGGGGGGGKSGKTYHRSTSRVFDEDMEIFISRALELKEEG 60

Query: 61  NTLFQSRDLEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLAL 120
           N LFQ RD EGAML ++KALKLLP++HIDV+YLR++MA+CYMQMGL EYP AI ECNLAL
Sbjct: 61  NKLFQKRDHEGAMLSFDKALKLLPKDHIDVAYLRTSMASCYMQMGLGEYPNAISECNLAL 120

Query: 121 EVTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALETKGSK 180
           E +P+YSKAL++R+RCYEAL++LD A RD   VLNMEP N+ A EI +R+ + L  KG  
Sbjct: 121 EASPRYSKALVRRSRCYEALNKLDYAFRDARIVLNMEPGNVSANEIFDRVKKVLVDKGI- 180

Query: 181 DVDAEIKLPLDFVELP--AAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIV------- 240
           DVD   ++  DFV++    A   +K  KE+ RK K  +K+    +E K+ ++V       
Sbjct: 181 DVD---EMEKDFVDVQPVCAARLKKIVKERLRKSKKKKKSGGKDEELKSPKVVVVDKGDE 240

Query: 241 --------EEKADEI-VEEKAGDETVQE----KADKIIVEEEKAEETGEEKKAEDKLVVE 300
                   EEK+D+  ++ K G +  ++    K+DK   ++    + GEE+K EDK+VV 
Sbjct: 241 AEGRNKPKEEKSDKSDIDGKIGGKREEKKTSFKSDKGQKKKSGGNKAGEERKVEDKVVVM 300

Query: 301 EKI-----------AKTQEEIPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTST 360
           +K            +K +     +T+KLV  +DIRWAQLP+  T+  +R+VIRDRFP   
Sbjct: 301 DKEVIASEIVDGGGSKKEGATVTRTIKLVHGDDIRWAQLPLDSTVRLVRDVIRDRFPALR 360

Query: 361 AVLIKYRDEEGDLVTITTNEELRLAETSTESQGSVRFYIFEVSPEKDPFYERLNNNE-VA 420
             LIKYRD EGDLVTITT +ELRLA ++ +  GS+R YI EV+P+++P Y+ ++N E   
Sbjct: 361 GFLIKYRDTEGDLVTITTTDELRLAASTHDKLGSLRLYIAEVNPDQEPTYDGMSNTESTD 420

Query: 421 KCKVEQSSVFENGHVLR-AKEMKMSSCIDDWIIHFAQLFINHVGFESGPYLDLHDLGMKL 480
           K     SS+ +NG V       K S C ++WI  FAQLF NHVGF+S  Y+DLHDLGMKL
Sbjct: 421 KVSKRLSSLADNGSVGEYVGSDKASGCFENWIFQFAQLFKNHVGFDSDSYVDLHDLGMKL 480

Query: 481 YTEAVEETVTSEEAQGLFDLAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLF 540
           YTEA+E+ VT E+AQ LF +AA+KF EM ALAL  WGNV MSKARK+V   + AS+  + 
Sbjct: 481 YTEAMEDAVTGEDAQELFQIAADKFQEMGALALLNWGNVHMSKARKQVCIPEDASREAII 540

Query: 541 DQIKAAYDWVEKEYAEAGRIYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDS 600
           + ++AA+ W + EY +A   Y+ AI++KPDFYE  LALGQ+QFE AKL W +A+ S VD 
Sbjct: 541 EAVEAAFVWTQNEYNKAAEKYEEAIKVKPDFYEALLALGQEQFEHAKLCWYHALKSKVDL 600

Query: 601 KTLPCTEVLKLYNNAEDNMETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDIS 660
           ++    EVLKLYN AED+ME GM++WEE EE R    SK    K  L+K+ LD L  + S
Sbjct: 601 ESEASQEVLKLYNKAEDSMERGMQIWEEMEECRLNGISKLDKHKNMLRKLELDELFSEAS 660

Query: 661 AAEAAEQAKNMRSHINILWGTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIA 720
             E  EQ  NM S IN+LWG++LYERSI+E+K+GLP W ECLEVAVEKFELAGASATDIA
Sbjct: 661 EEETVEQTANMSSQINLLWGSLLYERSIVEYKLGLPTWDECLEVAVEKFELAGASATDIA 720

Query: 721 VMIKNHCSSNNAHE-----------------------------GLGFKIDEIVQAWNEMY 739
           VM+KNHCSS +A E                             G+GFKIDEIVQAWNEMY
Sbjct: 721 VMVKNHCSSESALEGNQFLARIPNSGQVTTQWFSVYNNLRTNAGMGFKIDEIVQAWNEMY 780

BLAST of Spg022300 vs. TAIR 10
Match: AT5G20360.1 (Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide repeat (TPR)-containing protein )

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-149
Identity = 305/700 (43.57%), Postives = 460/700 (65.71%), Query Frame = 0

Query: 42  ITMSQVLKEEGNTLFQSRDLEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEY 101
           ++ +Q LKEEGN LFQ RD +GAM KY +A+K+LP++H++VS++R+N+A+CYMQ+   E+
Sbjct: 123 VSKAQGLKEEGNKLFQKRDYDGAMFKYGEAIKILPKDHVEVSHVRANVASCYMQLEPGEF 182

Query: 102 PRAIHECNLALEVTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISER 161
            +AIHEC+LAL VTP ++KALLKRARCYEAL++LDLALRDV  V  ++PKN MA EI E+
Sbjct: 183 AKAIHECDLALSVTPDHNKALLKRARCYEALNKLDLALRDVCMVSKLDPKNPMASEIVEK 242

Query: 162 LTEALETKGSKDVDAEIKLPLDFVE----LPAAVLPQKRPKEKSRKKKNNQKTKETIDEK 221
           L   LE+KG +  ++ I+LP D+VE     PAA+  +       + KK+NQ  +++  E 
Sbjct: 243 LKRTLESKGLRINNSVIELPPDYVEPVGASPAALWAKLGKVRVKKTKKSNQVEEKS--EG 302

Query: 222 KADEIVEEKADEIVEEKAGDETVQEKADKIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQ 281
           + +++  EK + ++ EK G E ++ K     V+ +++++  +  K ++K+++EE++    
Sbjct: 303 EGEDVEPEKKNNVLAEK-GKEKIKMK-----VKGKQSDKRSDTSKEQEKVIIEEELLVIG 362

Query: 282 EEIPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITT 341
            E   K VK V+ +DIR A+LPI CTL +LREV+ +RFP+  AV IKYRD+EGDLVTITT
Sbjct: 363 VEDVNKDVKFVYSDDIRLAELPINCTLFKLREVVHERFPSLRAVHIKYRDQEGDLVTITT 422

Query: 342 NEELRLAETSTESQGSVRFYIFEVSPEKDPFYERLNNNEVAKCKVEQSSVFENGHVLRAK 401
           +EELR++E S+ SQG++RFY+ EVSPE+DPF+ RL   E+ K K+   S        +AK
Sbjct: 423 DEELRMSEVSSRSQGTMRFYVVEVSPEQDPFFGRL--VEMKKLKITADS-------FKAK 482

Query: 402 EMKMSSC-IDDWIIHFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFD 461
                 C ++DW+I FA LF      +S   L+L +LGMKL +EA+EE VTS+ AQG FD
Sbjct: 483 VNGRGGCKVEDWMIEFAHLFKIQARIDSDRCLNLQELGMKLNSEAMEEVVTSDAAQGPFD 542

Query: 462 LAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGR 521
            AA++F E+AA +L   G V MS ARK++    G S   + +Q+K AY+  +KE+A A  
Sbjct: 543 RAAQQFQEVAARSLLNLGYVHMSGARKRLSLLQGVSGESVSEQVKTAYECAKKEHANAKE 602

Query: 522 IYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEDNM 581
            Y+ A++IKP+ +E +LALG QQFE+A+LSW Y + S++D KT P  +V++ Y +AE N+
Sbjct: 603 KYEEAMKIKPECFEVFLALGLQQFEEARLSWYYVLVSHLDLKTWPYADVVQFYQSAESNI 662

Query: 582 ETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINILW 641
           +  M++ E  E  +  E S++        +  L    ++      A++A  ++S I+IL 
Sbjct: 663 KKSMEVLENLETGKESEPSQAGKTDCLTHEKDLGSSTQN----NPAKEAGRLKSWIDILL 722

Query: 642 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 701
             +LYERSI+E+K+  P W E LE A+EKFELAG    D+  +I     + N    + F 
Sbjct: 723 CAVLYERSIMEYKLDQPFWRESLEAAMEKFELAGTCKDDVVEIISEDYVAGNTLRDIRFH 782

Query: 702 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHV 737
           ++EI+Q ++E+YEA+    G+PS +LE + +RR   I+HV
Sbjct: 783 MEEIIQIFDEIYEAKHWTNGIPSDQLEEILKRRAENIFHV 801