Spg022183 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg022183
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionvacuolar protein sorting-associated protein 27-like isoform X2
Locationscaffold2: 9903708 .. 9904844 (+)
RNA-Seq ExpressionSpg022183
SyntenySpg022183
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCACAGGGCGCTTGCCGCCAGCCCTGCGCGCGCGCGCACTGTCTGTGTCGCGCGCGCGCGCGCTGCTCGTGACCCGCGCGCACGATCGCGCGCGCGCGCCCCGTCCGTCTGCGCGCCAGCCTGCGCGCGCTTCCAGTCGTCCGTGCGCGCGCCCCTTGCGCACGCTGCCTGCCGCCCGCGCGCGCGCGCCCCTGCCTGCCGTGCGCGTGCGTGCGGCCTTGCGCCTGCCTGGCGCTGGGCAGCGTCTGGCGCCCCTCTGTGCGCCAGTGCGCCCAGCTGCGCGCCACCTGCGCGTGCTGGGCGTGTCTGCTGGGCGTGGGCAGCAAGCAACGCCCACTTCGCCCCCTTAGCAGCCACCCTATGCCATCAGTGGCCCTCCTGCCCCTCATGCCTGCCGTGCATGTTGTCATGAGTGCCTTGGCACGTTTGATCCCACTCCGCCTACCTTGCGCTTCACCTGTGTGCCGCCCACGCACACTCCTCGACTTGGGTGGTTCAAGGACCGCTCCACCCATCGAGTGTCCAAGTTTTAGGGTCGTGACACGTGCCCCCTATGAAACTTGATTATTCTTGTGGCAGAAGAAAACAACTTCCACCTCTACTCAGTACAATTCAAAATCAAAGAAGGCTGACTCCAATCATAGGAATAAAGGATCGACCTCCAACAGCAAGCTTCAGTATGCTATTTGGAGTTTTTGTTTTATTCTATATTCGAGCCTGTCTTCTCTTTCTGGCCCTGTATGTGGGAAAAGTAAGTTAATCATACTCCTTCTATGCGTACGTATGTCTTGGGTTAAGTGACATATTTTCTCATTGTTTATATTCCTGTGTGTGTAATAGGTATCAAATTACCCTCTTTATCACTGTTCGTATTATTGATTTCGTAGATAAGTCCAATCTTTAATCTGCCGTTTGTAATTTATATTATTAAATAGGAATCTTGCAGAGGAATCGAGGATTAATTATGAAGCAAGCAGCGAGGTATGTAGTCTGTGTCATCTCGATATTTATATGGTGTTTTTCAAAGGGTTTTCAAGTTCTGCTTCATCTCTAGAGTTTCATATCCTTGCAGGGGTTAAGGGAAGCAATCAAGAATGGCGACACTCATGCTGTCAGGAAACTTTTGAGTGAG

mRNA sequence

ATGGCGCACAGGGCGCTTGCCGCCAGCCCTGCGCGCGCGCGCACTGTCTGTGTCGCGCGCGCGCGCGCTGCTCGTGACCCGCGCGCACGATCGCGCGCGCGCGCCCCGTCCGTCTGCGCGCCAGCCTGCGCGCGCTTCCAGTCGTCCGTGCGCGCGCCCCTTGCGCACGCTGCCTGCCGCCCGCGCGCGCGCGCCCCTGCCTGCCGTGCGCGTGCGTGCGGCCTTGCGCCTGCCTGGCGCTGGGCAGCGTCTGGCGCCCCTCTGTGCGCCAGTGCGCCCAGCTGCGCGCCACCTGCGCGTGCTGGGCGTGTCTGCTGGGCGTGGGCAGCAAGCAACGCCCACTTCGCCCCCTTAGCAGCCACCCTATGCCATCAGTGGCCCTCCTGCCCCTCATGCCTGCCGTGCATGAATCTTGCAGAGGAATCGAGGATTAATTATGAAGCAAGCAGCGAGGGGTTAAGGGAAGCAATCAAGAATGGCGACACTCATGCTGTCAGGAAACTTTTGAGTGAG

Coding sequence (CDS)

ATGGCGCACAGGGCGCTTGCCGCCAGCCCTGCGCGCGCGCGCACTGTCTGTGTCGCGCGCGCGCGCGCTGCTCGTGACCCGCGCGCACGATCGCGCGCGCGCGCCCCGTCCGTCTGCGCGCCAGCCTGCGCGCGCTTCCAGTCGTCCGTGCGCGCGCCCCTTGCGCACGCTGCCTGCCGCCCGCGCGCGCGCGCCCCTGCCTGCCGTGCGCGTGCGTGCGGCCTTGCGCCTGCCTGGCGCTGGGCAGCGTCTGGCGCCCCTCTGTGCGCCAGTGCGCCCAGCTGCGCGCCACCTGCGCGTGCTGGGCGTGTCTGCTGGGCGTGGGCAGCAAGCAACGCCCACTTCGCCCCCTTAGCAGCCACCCTATGCCATCAGTGGCCCTCCTGCCCCTCATGCCTGCCGTGCATGAATCTTGCAGAGGAATCGAGGATTAATTATGAAGCAAGCAGCGAGGGGTTAAGGGAAGCAATCAAGAATGGCGACACTCATGCTGTCAGGAAACTTTTGAGTGAG

Protein sequence

MAHRALAASPARARTVCVARARAARDPRARSRARAPSVCAPACARFQSSVRAPLAHAACRPRARAPACRARACGLAPAWRWAASGAPLCASAPSCAPPARAGRVCWAWAASNAHFAPLAATLCHQWPSCPSCLPCMNLAEESRINYEASSEGLREAIKNGDTHAVRKLLSE
Homology
[Close]
BLAST of Spg022183 vs. NCBI nr
Match: XP_022929532.1 (vacuolar protein sorting-associated protein 27-like isoform X1 [Cucurbita moschata] >XP_022929540.1 vacuolar protein sorting-associated protein 27-like isoform X1 [Cucurbita moschata] >KAG7021998.1 Rabankyrin-5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-06
Identity = 31/35 (88.57%), Postives = 33/35 (94.29%), Query Frame = 0 

Query: 137 NLAEESRINYEASSEGLREAIKNGDTHAVRKLLSE 172
           N A+ESRINYEAS EGLREAIKNGDTHAV+KLLSE
Sbjct: 181 NPADESRINYEASGEGLREAIKNGDTHAVKKLLSE 215

[Close]
BLAST of Spg022183 vs. NCBI nr
Match: KAG6588113.1 (Rabankyrin-5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-06
Identity = 31/35 (88.57%), Postives = 33/35 (94.29%), Query Frame = 0 

Query: 137 NLAEESRINYEASSEGLREAIKNGDTHAVRKLLSE 172
           N A+ESRINYEAS EGLREAIKNGDTHAV+KLLSE
Sbjct: 175 NPADESRINYEASGEGLREAIKNGDTHAVKKLLSE 209

[Close]
BLAST of Spg022183 vs. NCBI nr
Match: XP_022929552.1 (potassium channel KOR2-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-06
Identity = 31/35 (88.57%), Postives = 33/35 (94.29%), Query Frame = 0 

Query: 137 NLAEESRINYEASSEGLREAIKNGDTHAVRKLLSE 172
           N A+ESRINYEAS EGLREAIKNGDTHAV+KLLSE
Sbjct: 175 NPADESRINYEASGEGLREAIKNGDTHAVKKLLSE 209

[Close]
BLAST of Spg022183 vs. NCBI nr
Match: XP_022929548.1 (vacuolar protein sorting-associated protein 27-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-06
Identity = 31/35 (88.57%), Postives = 33/35 (94.29%), Query Frame = 0 

Query: 137 NLAEESRINYEASSEGLREAIKNGDTHAVRKLLSE 172
           N A+ESRINYEAS EGLREAIKNGDTHAV+KLLSE
Sbjct: 180 NPADESRINYEASGEGLREAIKNGDTHAVKKLLSE 214

[Close]
BLAST of Spg022183 vs. NCBI nr
Match: XP_038878461.1 (vacuolar protein sorting-associated protein 27 [Benincasa hispida])

HSP 1 Score: 62.4 bits (150), Expect = 4.4e-06
Identity = 31/35 (88.57%), Postives = 33/35 (94.29%), Query Frame = 0 

Query: 137 NLAEESRINYEASSEGLREAIKNGDTHAVRKLLSE 172
           NLA+ESRINYEAS EGLREAIKNGDT AV+KLLSE
Sbjct: 175 NLADESRINYEASGEGLREAIKNGDTIAVKKLLSE 209

[Close]
BLAST of Spg022183 vs. ExPASy TrEMBL
Match: A0A6J1EUN7 (vacuolar protein sorting-associated protein 27-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436069 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 7.3e-07
Identity = 31/35 (88.57%), Postives = 33/35 (94.29%), Query Frame = 0 

Query: 137 NLAEESRINYEASSEGLREAIKNGDTHAVRKLLSE 172
           N A+ESRINYEAS EGLREAIKNGDTHAV+KLLSE
Sbjct: 181 NPADESRINYEASGEGLREAIKNGDTHAVKKLLSE 215

[Close]
BLAST of Spg022183 vs. ExPASy TrEMBL
Match: A0A6J1ESF6 (vacuolar protein sorting-associated protein 27-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436069 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 7.3e-07
Identity = 31/35 (88.57%), Postives = 33/35 (94.29%), Query Frame = 0 

Query: 137 NLAEESRINYEASSEGLREAIKNGDTHAVRKLLSE 172
           N A+ESRINYEAS EGLREAIKNGDTHAV+KLLSE
Sbjct: 180 NPADESRINYEASGEGLREAIKNGDTHAVKKLLSE 214

[Close]
BLAST of Spg022183 vs. ExPASy TrEMBL
Match: A0A6J1EN28 (potassium channel KOR2-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111436069 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 7.3e-07
Identity = 31/35 (88.57%), Postives = 33/35 (94.29%), Query Frame = 0 

Query: 137 NLAEESRINYEASSEGLREAIKNGDTHAVRKLLSE 172
           N A+ESRINYEAS EGLREAIKNGDTHAV+KLLSE
Sbjct: 175 NPADESRINYEASGEGLREAIKNGDTHAVKKLLSE 209

[Close]
BLAST of Spg022183 vs. ExPASy TrEMBL
Match: A0A5A7UKZ2 (Vacuolar protein sorting-associated protein 27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001170 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.1e-06
Identity = 31/35 (88.57%), Postives = 33/35 (94.29%), Query Frame = 0 

Query: 137 NLAEESRINYEASSEGLREAIKNGDTHAVRKLLSE 172
           NLA+ESRINYEAS EGLREAIKNGDT AV+KLLSE
Sbjct: 175 NLADESRINYEASGEGLREAIKNGDTIAVKKLLSE 209

[Close]
BLAST of Spg022183 vs. ExPASy TrEMBL
Match: A0A1S4DY61 (LOW QUALITY PROTEIN: vacuolar protein sorting-associated protein 27 OS=Cucumis melo OX=3656 GN=LOC103492539 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.1e-06
Identity = 31/35 (88.57%), Postives = 33/35 (94.29%), Query Frame = 0 

Query: 137 NLAEESRINYEASSEGLREAIKNGDTHAVRKLLSE 172
           NLA+ESRINYEAS EGLREAIKNGDT AV+KLLSE
Sbjct: 175 NLADESRINYEASGEGLREAIKNGDTIAVKKLLSE 209

[Close]
BLAST of Spg022183 vs. TAIR 10
Match: AT2G26210.1 (Ankyrin repeat family protein )

HSP 1 Score: 41.6 bits (96), Expect = 7.5e-04
Identity = 19/27 (70.37%), Postives = 22/27 (81.48%), Query Frame = 0 

Query: 145 NYEASSEGLREAIKNGDTHAVRKLLSE 172
           +YEAS EGLREAIKNGD+   +KLL E
Sbjct: 98  DYEASGEGLREAIKNGDSAGAKKLLKE 124

[Close]
BLAST of Spg022183 vs. TAIR 10
Match: AT2G26210.2 (Ankyrin repeat family protein )

HSP 1 Score: 41.6 bits (96), Expect = 7.5e-04
Identity = 19/27 (70.37%), Postives = 22/27 (81.48%), Query Frame = 0 

Query: 145 NYEASSEGLREAIKNGDTHAVRKLLSE 172
           +YEAS EGLREAIKNGD+   +KLL E
Sbjct: 75  DYEASGEGLREAIKNGDSAGAKKLLKE 101

[Close]
BLAST of Spg022183 vs. TAIR 10
Match: AT2G26210.3 (Ankyrin repeat family protein )

HSP 1 Score: 41.6 bits (96), Expect = 7.5e-04
Identity = 19/27 (70.37%), Postives = 22/27 (81.48%), Query Frame = 0 

Query: 145 NYEASSEGLREAIKNGDTHAVRKLLSE 172
           +YEAS EGLREAIKNGD+   +KLL E
Sbjct: 75  DYEASGEGLREAIKNGDSAGAKKLLKE 101

The following BLAST results are available for this feature:
BLAST of Spg022183 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022929532.11.5e-0688.57vacuolar protein sorting-associated protein 27-like isoform X1 [Cucurbita moscha... [more]
KAG6588113.11.5e-0688.57Rabankyrin-5, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022929552.11.5e-0688.57potassium channel KOR2-like isoform X3 [Cucurbita moschata][more]
XP_022929548.11.5e-0688.57vacuolar protein sorting-associated protein 27-like isoform X2 [Cucurbita moscha... [more]
XP_038878461.14.4e-0688.57vacuolar protein sorting-associated protein 27 [Benincasa hispida][more]
BLAST of Spg022183 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Spg022183 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1EUN77.3e-0788.57vacuolar protein sorting-associated protein 27-like isoform X1 OS=Cucurbita mosc... [more]
A0A6J1ESF67.3e-0788.57vacuolar protein sorting-associated protein 27-like isoform X2 OS=Cucurbita mosc... [more]
A0A6J1EN287.3e-0788.57potassium channel KOR2-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A5A7UKZ22.1e-0688.57Vacuolar protein sorting-associated protein 27 OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S4DY612.1e-0688.57LOW QUALITY PROTEIN: vacuolar protein sorting-associated protein 27 OS=Cucumis m... [more]
BLAST of Spg022183 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G26210.17.5e-0470.37Ankyrin repeat family protein [more]
AT2G26210.27.5e-0470.37Ankyrin repeat family protein [more]
AT2G26210.37.5e-0470.37Ankyrin repeat family protein [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg022183.1Spg022183.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006623 protein targeting to vacuole
biological_process GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
cellular_component GO:0033565 ESCRT-0 complex
molecular_function GO:0046872 metal ion binding
molecular_function GO:0032266 phosphatidylinositol-3-phosphate binding
molecular_function GO:0043130 ubiquitin binding