Spg022062 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg022062 vs. NCBI nr
Match: XP_023531376.1 (cell division cycle protein 27 homolog B-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1422.1 bits (3680), Expect = 0.0e+00
Identity = 719/756 (95.11%), Postives = 739/756 (97.75%), Query Frame = 0

Query: 1   MEAILTDCVHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60
           MEA LTDCVHHSL HFMHRNAIFMCERLC+EFPSETNMQLLAGCFLHNNQAYAAY ILKG
Sbjct: 1   MEATLTDCVHHSLRHFMHRNAIFMCERLCAEFPSETNMQLLAGCFLHNNQAYAAYRILKG 60

Query: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120
           TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA
Sbjct: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120

Query: 121 IQHFQQALSLDPLMWCAYEELCILGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTLSDE 180
           IQHFQ ALSLDPLMWCAYEELC+LGAAEDASSVFGEAAVLCIQKQYLHHRSENLQT +D+
Sbjct: 121 IQHFQLALSLDPLMWCAYEELCVLGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTSNDD 180

Query: 181 LNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAASQIVNGGSNISFYNTP 240
           +NSAS+RN++SDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGA SQ VNGGSNISFYNTP
Sbjct: 181 MNSASSRNSNSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAGSQAVNGGSNISFYNTP 240

Query: 241 SPVAAQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTVNSIIQAPRRKFVDEGKLRKIS 300
           SPVAAQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRST+NSIIQAPRRKFVDEGKLRKIS
Sbjct: 241 SPVAAQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTINSIIQAPRRKFVDEGKLRKIS 300

Query: 301 GRLFSDSGPRRSSRLAGEAGANTNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVVRKG 360
           GRLFSDSGPRRSSRLAGEAGANTNVSAA AANNGTTN+TKYLG SK+NSI FRS+ VRKG
Sbjct: 301 GRLFSDSGPRRSSRLAGEAGANTNVSAAGAANNGTTNATKYLGSSKMNSITFRSVAVRKG 360

Query: 361 QSFANENIDEGIQNEAFDESRSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAKIIN 420
           QSFANEN+DEGIQNEAFD+SRSNASLSISSSSPS+DNRTLEQGA KSVGGSLT+DAKIIN
Sbjct: 361 QSFANENVDEGIQNEAFDDSRSNASLSISSSSPSSDNRTLEQGAPKSVGGSLTSDAKIIN 420

Query: 421 GASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLE 480
           GASEIL LLRILGEGYRLSCLFRCQDALDVYLKLPYKHY+TGWVLSQVGKVYFELVDYLE
Sbjct: 421 GASEILCLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYNTGWVLSQVGKVYFELVDYLE 480

Query: 481 ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMGNCY 540
           ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELIS DRLAPQSWCAMGNCY
Sbjct: 481 ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISTDRLAPQSWCAMGNCY 540

Query: 541 SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNS 600
           SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNS
Sbjct: 541 SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNS 600

Query: 601 WYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAIL 660
           WYGLGMIYL+QEK EFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAIL
Sbjct: 601 WYGLGMIYLKQEKLEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAIL 660

Query: 661 ADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAM 720
           ADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMG IYKRRYMH+KAM
Sbjct: 661 ADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGNIYKRRYMHDKAM 720

Query: 721 LHFGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL 757
           LHFG+ALDLKPSAADVATIKAAIEKL VPDEIEDNL
Sbjct: 721 LHFGIALDLKPSAADVATIKAAIEKLLVPDEIEDNL 756

BLAST of Spg022062 vs. NCBI nr
Match: XP_022933562.1 (cell division cycle protein 27 homolog B-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1419.4 bits (3673), Expect = 0.0e+00
Identity = 717/756 (94.84%), Postives = 739/756 (97.75%), Query Frame = 0

Query: 1   MEAILTDCVHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60
           MEA LTDCVHHSL HFM+RNAIFMCERLC+EFPSETNMQLLAGCFLHNNQAYAAY ILKG
Sbjct: 1   MEATLTDCVHHSLRHFMYRNAIFMCERLCAEFPSETNMQLLAGCFLHNNQAYAAYRILKG 60

Query: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120
           TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA
Sbjct: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120

Query: 121 IQHFQQALSLDPLMWCAYEELCILGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTLSDE 180
           IQHFQ ALSLDPLMWCAYEELC+LGAAEDASSVFGEAAVLCIQKQYLHHRSENLQT +D+
Sbjct: 121 IQHFQLALSLDPLMWCAYEELCVLGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTSNDD 180

Query: 181 LNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAASQIVNGGSNISFYNTP 240
           +NSAS+RN++SDDVRTRQSKHTQSNNLRDIPTNYHGAVN+GGA SQ +NGGSNISFYNTP
Sbjct: 181 MNSASSRNSNSDDVRTRQSKHTQSNNLRDIPTNYHGAVNMGGAGSQSINGGSNISFYNTP 240

Query: 241 SPVAAQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTVNSIIQAPRRKFVDEGKLRKIS 300
           SPVAAQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRST+NSIIQAPRRKFVDEGKLRKIS
Sbjct: 241 SPVAAQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTINSIIQAPRRKFVDEGKLRKIS 300

Query: 301 GRLFSDSGPRRSSRLAGEAGANTNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVVRKG 360
           GRLFSDSGPRRSSRLAGEAGANTNVSAA AANNGTTNSTKYLG SK+NSI FRS+ VRKG
Sbjct: 301 GRLFSDSGPRRSSRLAGEAGANTNVSAAGAANNGTTNSTKYLGSSKMNSITFRSVAVRKG 360

Query: 361 QSFANENIDEGIQNEAFDESRSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAKIIN 420
           QSFANEN+DEGIQNEAFD+SRSNASLSISSSSPS+DNRTLEQGA KSVGGSLT+DAKIIN
Sbjct: 361 QSFANENVDEGIQNEAFDDSRSNASLSISSSSPSSDNRTLEQGAPKSVGGSLTSDAKIIN 420

Query: 421 GASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLE 480
           GASEIL LLRILGEGYRLSCLFRCQDALDVYLKLPYKHY+TGWVLSQVGKVYFELVDYLE
Sbjct: 421 GASEILCLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYNTGWVLSQVGKVYFELVDYLE 480

Query: 481 ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMGNCY 540
           ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELIS DRLAPQSWCAMGNCY
Sbjct: 481 ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISTDRLAPQSWCAMGNCY 540

Query: 541 SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNS 600
           SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNS
Sbjct: 541 SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNS 600

Query: 601 WYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAIL 660
           WYGLGMIYL+QEK EFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAIL
Sbjct: 601 WYGLGMIYLKQEKLEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAIL 660

Query: 661 ADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAM 720
           ADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMG IYKRRYMH+KAM
Sbjct: 661 ADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGNIYKRRYMHDKAM 720

Query: 721 LHFGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL 757
           LHFG+ALDLKPSAADVATIKAAIEKL VPDEIEDNL
Sbjct: 721 LHFGIALDLKPSAADVATIKAAIEKLLVPDEIEDNL 756

BLAST of Spg022062 vs. NCBI nr
Match: KAG6589947.1 (Cell division cycle protein 27-like B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1418.3 bits (3670), Expect = 0.0e+00
Identity = 723/756 (95.63%), Postives = 738/756 (97.62%), Query Frame = 0

Query: 1   MEAILTDCVHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60
           ME IL DCVHHSL HFM+RNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG
Sbjct: 1   MEVILADCVHHSLRHFMYRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60

Query: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120
           TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA
Sbjct: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120

Query: 121 IQHFQQALSLDPLMWCAYEELCILGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTLSDE 180
           IQHFQQALS+DPLMWCAYEELCILGAAEDASSVFGEAAVL IQKQYLHHRSENLQTL+D+
Sbjct: 121 IQHFQQALSIDPLMWCAYEELCILGAAEDASSVFGEAAVLSIQKQYLHHRSENLQTLNDD 180

Query: 181 LNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAASQIVNGGSNISFYNTP 240
           LNSASARNN+SD+VRTRQ KHTQSNNLRDIPTNY G VNLGGAASQIVNGGSNISFYNTP
Sbjct: 181 LNSASARNNNSDEVRTRQLKHTQSNNLRDIPTNYQGTVNLGGAASQIVNGGSNISFYNTP 240

Query: 241 SPVAAQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTVNSIIQAPRRKFVDEGKLRKIS 300
           SPVAAQLSAIAPPPLCRNTQQNGSS+NSLGADSCSRSTVNSIIQAPRRKFVDEGKLRKIS
Sbjct: 241 SPVAAQLSAIAPPPLCRNTQQNGSSINSLGADSCSRSTVNSIIQAPRRKFVDEGKLRKIS 300

Query: 301 GRLFSDSGPRRSSRLAGEAGANTNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVVRKG 360
           GRLFSDSGPRRSSRLAGEA ANTNVS   AANNGTTNSTKYLGGSKLNSI  RSM VRKG
Sbjct: 301 GRLFSDSGPRRSSRLAGEAVANTNVS---AANNGTTNSTKYLGGSKLNSITLRSMAVRKG 360

Query: 361 QSFANENIDEGIQNEAFDESRSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAKIIN 420
           QSFANENIDEGIQNEAFD+SRSNASLS+SSSSPS+ NRTLEQ ATKSVGGSLTNDAKIIN
Sbjct: 361 QSFANENIDEGIQNEAFDDSRSNASLSVSSSSPSSGNRTLEQEATKSVGGSLTNDAKIIN 420

Query: 421 GASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLE 480
           GASEILGLLRILGEG+RLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLE
Sbjct: 421 GASEILGLLRILGEGHRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLE 480

Query: 481 ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMGNCY 540
           ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELIS DRLAPQSWCAMGNCY
Sbjct: 481 ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISTDRLAPQSWCAMGNCY 540

Query: 541 SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNS 600
           SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGI+SYQSALRVDSRHYNS
Sbjct: 541 SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIQSYQSALRVDSRHYNS 600

Query: 601 WYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAIL 660
           WYGLGMIYLRQEK EFSEHHFRMAFQINPRSSVVMSYLGTSLHAL+RSEDAMMMMEKAIL
Sbjct: 601 WYGLGMIYLRQEKLEFSEHHFRMAFQINPRSSVVMSYLGTSLHALKRSEDAMMMMEKAIL 660

Query: 661 ADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAM 720
           ADKKNPLPMYQKANIL+SLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAM
Sbjct: 661 ADKKNPLPMYQKANILISLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAM 720

Query: 721 LHFGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL 757
           L+FGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL
Sbjct: 721 LNFGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL 753

BLAST of Spg022062 vs. NCBI nr
Match: XP_023516280.1 (cell division cycle protein 27 homolog B-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 722/756 (95.50%), Postives = 737/756 (97.49%), Query Frame = 0

Query: 1   MEAILTDCVHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60
           ME IL DCVHHSL HFM+RNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG
Sbjct: 1   MEVILADCVHHSLRHFMYRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60

Query: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120
           T+MAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA
Sbjct: 61  TKMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120

Query: 121 IQHFQQALSLDPLMWCAYEELCILGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTLSDE 180
           IQHFQQALS+DPLMWCAYEELCILGAAEDASSVFGEAAVL IQKQYLHHRSENLQTL+D+
Sbjct: 121 IQHFQQALSIDPLMWCAYEELCILGAAEDASSVFGEAAVLSIQKQYLHHRSENLQTLNDD 180

Query: 181 LNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAASQIVNGGSNISFYNTP 240
           LNSASARNN+SDDVRTRQ KHTQSNNLRDIPTNY G VNLGGAASQIVNGGSNISFYNTP
Sbjct: 181 LNSASARNNNSDDVRTRQLKHTQSNNLRDIPTNYQGTVNLGGAASQIVNGGSNISFYNTP 240

Query: 241 SPVAAQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTVNSIIQAPRRKFVDEGKLRKIS 300
           SPVAAQ+SAIAPPPLCRNTQQNGSS+NSLGADSCSRS VNSIIQAPRRKFVDEGKLRKIS
Sbjct: 241 SPVAAQVSAIAPPPLCRNTQQNGSSINSLGADSCSRSAVNSIIQAPRRKFVDEGKLRKIS 300

Query: 301 GRLFSDSGPRRSSRLAGEAGANTNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVVRKG 360
           GRLFSDSGPRRSSRLAGEA ANTNVS   AANNGTTNSTKYLGGSKLNSI  RSM VRKG
Sbjct: 301 GRLFSDSGPRRSSRLAGEAVANTNVS---AANNGTTNSTKYLGGSKLNSITLRSMAVRKG 360

Query: 361 QSFANENIDEGIQNEAFDESRSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAKIIN 420
           QSFANENIDEGIQNEAFD+SRSNASLS+SSSSPS+ NR LEQGATKSVGGSLTNDAKIIN
Sbjct: 361 QSFANENIDEGIQNEAFDDSRSNASLSVSSSSPSSGNRILEQGATKSVGGSLTNDAKIIN 420

Query: 421 GASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLE 480
           GASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLE
Sbjct: 421 GASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLE 480

Query: 481 ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMGNCY 540
           ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELIS DRLAPQSWCAMGNCY
Sbjct: 481 ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISTDRLAPQSWCAMGNCY 540

Query: 541 SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNS 600
           SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGI+SYQSALRVDSRHYNS
Sbjct: 541 SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIQSYQSALRVDSRHYNS 600

Query: 601 WYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAIL 660
           WYGLGMIYLRQEK EFSEHHFRMAFQINPRSSVVMSYLGTSLHAL+RSEDAMMMMEKAIL
Sbjct: 601 WYGLGMIYLRQEKLEFSEHHFRMAFQINPRSSVVMSYLGTSLHALKRSEDAMMMMEKAIL 660

Query: 661 ADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAM 720
           ADKKNPLPMYQKANIL+SLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAM
Sbjct: 661 ADKKNPLPMYQKANILISLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAM 720

Query: 721 LHFGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL 757
           L+FGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL
Sbjct: 721 LNFGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL 753

BLAST of Spg022062 vs. NCBI nr
Match: XP_023531375.1 (cell division cycle protein 27 homolog B-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1416.7 bits (3666), Expect = 0.0e+00
Identity = 719/759 (94.73%), Postives = 739/759 (97.36%), Query Frame = 0

Query: 1   MEAILTDCVHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60
           MEA LTDCVHHSL HFMHRNAIFMCERLC+EFPSETNMQLLAGCFLHNNQAYAAY ILKG
Sbjct: 1   MEATLTDCVHHSLRHFMHRNAIFMCERLCAEFPSETNMQLLAGCFLHNNQAYAAYRILKG 60

Query: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120
           TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA
Sbjct: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120

Query: 121 IQHFQQALSLDPLMWCAYEELCILGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTLSDE 180
           IQHFQ ALSLDPLMWCAYEELC+LGAAEDASSVFGEAAVLCIQKQYLHHRSENLQT +D+
Sbjct: 121 IQHFQLALSLDPLMWCAYEELCVLGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTSNDD 180

Query: 181 LNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAASQIVNGGSNISFYNTP 240
           +NSAS+RN++SDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGA SQ VNGGSNISFYNTP
Sbjct: 181 MNSASSRNSNSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAGSQAVNGGSNISFYNTP 240

Query: 241 SPVAA---QLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTVNSIIQAPRRKFVDEGKLR 300
           SPVAA   QLSAIAPPPLCRNTQQNGSSLNSLGADSCSRST+NSIIQAPRRKFVDEGKLR
Sbjct: 241 SPVAAQNLQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTINSIIQAPRRKFVDEGKLR 300

Query: 301 KISGRLFSDSGPRRSSRLAGEAGANTNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVV 360
           KISGRLFSDSGPRRSSRLAGEAGANTNVSAA AANNGTTN+TKYLG SK+NSI FRS+ V
Sbjct: 301 KISGRLFSDSGPRRSSRLAGEAGANTNVSAAGAANNGTTNATKYLGSSKMNSITFRSVAV 360

Query: 361 RKGQSFANENIDEGIQNEAFDESRSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAK 420
           RKGQSFANEN+DEGIQNEAFD+SRSNASLSISSSSPS+DNRTLEQGA KSVGGSLT+DAK
Sbjct: 361 RKGQSFANENVDEGIQNEAFDDSRSNASLSISSSSPSSDNRTLEQGAPKSVGGSLTSDAK 420

Query: 421 IINGASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVD 480
           IINGASEIL LLRILGEGYRLSCLFRCQDALDVYLKLPYKHY+TGWVLSQVGKVYFELVD
Sbjct: 421 IINGASEILCLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYNTGWVLSQVGKVYFELVD 480

Query: 481 YLEADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMG 540
           YLEADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELIS DRLAPQSWCAMG
Sbjct: 481 YLEADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISTDRLAPQSWCAMG 540

Query: 541 NCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRH 600
           NCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRH
Sbjct: 541 NCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRH 600

Query: 601 YNSWYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEK 660
           YNSWYGLGMIYL+QEK EFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEK
Sbjct: 601 YNSWYGLGMIYLKQEKLEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEK 660

Query: 661 AILADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHE 720
           AILADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMG IYKRRYMH+
Sbjct: 661 AILADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGNIYKRRYMHD 720

Query: 721 KAMLHFGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL 757
           KAMLHFG+ALDLKPSAADVATIKAAIEKL VPDEIEDNL
Sbjct: 721 KAMLHFGIALDLKPSAADVATIKAAIEKLLVPDEIEDNL 759

BLAST of Spg022062 vs. ExPASy Swiss-Prot
Match: Q8LGU6 (Cell division cycle protein 27 homolog B OS=Arabidopsis thaliana OX=3702 GN=CDC27B PE=1 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 2.3e-312
Identity = 550/758 (72.56%), Postives = 641/758 (84.56%), Query Frame = 0

Query: 1   MEAILTDCVHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60
           MEA+L DCV++SL HF+++NAIFMCERLC+EFPSE N+QLLA  +L NNQAY+AYH+LKG
Sbjct: 1   MEAMLVDCVNNSLRHFVYKNAIFMCERLCAEFPSEVNLQLLATSYLQNNQAYSAYHLLKG 60

Query: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120
           TQMAQSRYLFA+SCFQMDLL+EAE+ALCP NEPGAEIPNGAAGHYLLGLIY+YTDRR++A
Sbjct: 61  TQMAQSRYLFALSCFQMDLLNEAESALCPVNEPGAEIPNGAAGHYLLGLIYKYTDRRKNA 120

Query: 121 IQHFQQALSLDPLMWCAYEELCILGAAEDASSVFGEAAVLCIQKQYLHHRSEN--LQTLS 180
            Q F+Q+L++DPL+W AYEELCILGAAE+A++VFGE A L IQKQY+   S +  L T +
Sbjct: 121 AQQFKQSLTIDPLLWAAYEELCILGAAEEATAVFGETAALSIQKQYMQQLSTSLGLNTYN 180

Query: 181 DELNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAASQIVNGG-SNISFY 240
           +E NS S +N SS+D   RQSKHTQS+ L+DI  N+H         S  VNGG SN+SFY
Sbjct: 181 EERNSTSTKNTSSEDYSPRQSKHTQSHGLKDISGNFH---------SHGVNGGVSNMSFY 240

Query: 241 NTPSPVAAQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTVNSIIQAPRRKFVDEGKLR 300
           NTPSPVAAQLS IAPPPL RN Q   ++ NSL  DS  +STVNS +QAPRRKFVDEGKLR
Sbjct: 241 NTPSPVAAQLSGIAPPPLFRNFQPAVANPNSLITDSSPKSTVNSTLQAPRRKFVDEGKLR 300

Query: 301 KISGRLFSDSGPRRSSRLAGEAGANTNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVV 360
           KISGRLFSDSGPRRSSRL+ ++GAN N S A  + N   N++KYLGGSKL+S+A RS+ +
Sbjct: 301 KISGRLFSDSGPRRSSRLSADSGANINSSVATVSGN-VNNASKYLGGSKLSSLALRSVTL 360

Query: 361 RKGQSFANENIDEGIQNEAFDESRSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAK 420
           RKG S+ANEN+DEG++ E FD+SR N + S + S  SND    ++  T S+GG   +   
Sbjct: 361 RKGHSWANENMDEGVRGEPFDDSRPNTA-STTGSMASND----QEDETMSIGGIAMSSQT 420

Query: 421 IINGASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVD 480
           I  G SEIL LLR LGEG RLS ++RCQ+ALD Y+KLP+KHY+TGWVLSQVGK YFEL+D
Sbjct: 421 ITIGVSEILNLLRTLGEGCRLSYMYRCQEALDTYMKLPHKHYNTGWVLSQVGKAYFELID 480

Query: 481 YLEADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMG 540
           YLEA++AF LAR ASP+ LEGMD+YSTVLYHLKEDMKLSYLAQELIS DRLAPQSWCAMG
Sbjct: 481 YLEAEKAFRLARLASPYCLEGMDIYSTVLYHLKEDMKLSYLAQELISTDRLAPQSWCAMG 540

Query: 541 NCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRH 600
           NCYSLQKDHETALKNF RAVQLNPRFAYAHTLCGHEY  LEDFENG+KSYQ+ALRVD+RH
Sbjct: 541 NCYSLQKDHETALKNFLRAVQLNPRFAYAHTLCGHEYTTLEDFENGMKSYQNALRVDTRH 600

Query: 601 YNSWYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEK 660
           YN+WYGLGMIYLRQEK EFSEHHFRMAF INP SSV+MSYLGTSLHAL+RSE+A+ +ME+
Sbjct: 601 YNAWYGLGMIYLRQEKLEFSEHHFRMAFLINPSSSVIMSYLGTSLHALKRSEEALEIMEQ 660

Query: 661 AILADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHE 720
           AI+AD+KNPLPMYQKANILV LER DEAL+VLEELKEYAP ESSVYALMG+IYKRR MH+
Sbjct: 661 AIVADRKNPLPMYQKANILVCLERLDEALEVLEELKEYAPSESSVYALMGRIYKRRNMHD 720

Query: 721 KAMLHFGLALDLKPSAADVATIKAAIEKLHVPDEIEDN 756
           KAMLHFGLALD+KP A DVA IKAA+EKLHVPDEI+++
Sbjct: 721 KAMLHFGLALDMKPPATDVAAIKAAMEKLHVPDEIDES 743

BLAST of Spg022062 vs. ExPASy Swiss-Prot
Match: Q06AN9 (Cell division cycle protein 27 homolog A OS=Arabidopsis thaliana OX=3702 GN=CDC27A PE=1 SV=2)

HSP 1 Score: 643.7 bits (1659), Expect = 2.7e-183
Identity = 363/761 (47.70%), Postives = 491/761 (64.52%), Query Frame = 0

Query: 1   MEAILTDCVHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60
           ME +L +CV  +L+HFM  NAIF+CE L ++FPSE N+QLLA C+L N+QAY+AY+ILKG
Sbjct: 2   MENLLANCVQKNLNHFMFTNAIFLCELLLAQFPSEVNLQLLARCYLSNSQAYSAYYILKG 61

Query: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120
           ++  QSRYLFA SCF++DLL EAEAAL P  +   E+P GAAGHYLLGLIYRY+ R+  +
Sbjct: 62  SKTPQSRYLFAFSCFKLDLLGEAEAALLPCEDYAEEVPGGAAGHYLLGLIYRYSGRKNCS 121

Query: 121 IQHFQQALSLDPLMWCAYEELCILGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTLSDE 180
           IQ F+ ALS DPL W AY ELC LGAAE+AS+VFG  A   +QK  +  R    +  +  
Sbjct: 122 IQQFRMALSFDPLCWEAYGELCSLGAAEEASTVFGNVASQRLQKTCVEQRISFSEGAT-- 181

Query: 181 LNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAASQIVNGGSNISFYNTP 240
           ++  +  + +  D    Q++H    N +D+               Q+   G ++   NTP
Sbjct: 182 IDQITDSDKALKDTGLSQTEHIPGENQQDLKIMQQPGDIPPNTDRQLSTNGWDL---NTP 241

Query: 241 SPVAAQLSAIAPPPLCRNTQQ---NGSSLNSLGADSCSRSTVNSIIQAPRRKFVDEGKLR 300
           SPV  Q+    PP L +N ++    GS ++  G            ++  RR F  E    
Sbjct: 242 SPVLLQVMDALPPLLLKNMRRPAVEGSLMSVHG------------VRVRRRNFFSE---- 301

Query: 301 KISGRLFSDSGPRRSSRLAGEAGANTNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVV 360
           ++S     +SG RRS+R+A             + + G  +   +L  S+ N     S ++
Sbjct: 302 ELSAEAQEESGRRRSARIAARK------KNPMSQSFGKDSHWLHLSPSESNYAPSLSSMI 361

Query: 361 RKGQSFANENIDEGIQNEAFDESRSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAK 420
            K +      I    +     +S S+   S+     SN +             S  +   
Sbjct: 362 GKCR------IQSSKEATTSGQSVSDIGSSVDDEEKSNPSE------------SSPDRFS 421

Query: 421 IINGASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVD 480
           +I+G SE+L LL+ILG+G+R   +++CQ+AL  Y KL  K Y+T WVL QVGK YFEL D
Sbjct: 422 LISGISEVLSLLKILGDGHRHLHMYKCQEALLAYQKLSQKQYNTHWVLMQVGKAYFELQD 481

Query: 481 YLEADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMG 540
           Y  AD +F+LA    P++LEGMD YSTVLYHLKE+M+L YLAQELIS DRL+P+SWCA+G
Sbjct: 482 YFNADSSFTLAHQKYPYALEGMDTYSTVLYHLKEEMRLGYLAQELISVDRLSPESWCAVG 541

Query: 541 NCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRH 600
           NCYSL+KDH+TALK FQRA+QLN RF YAHTLCGHE+ ALE+FE+  + Y+ AL +D+RH
Sbjct: 542 NCYSLRKDHDTALKMFQRAIQLNERFTYAHTLCGHEFAALEEFEDAERCYRKALGIDTRH 601

Query: 601 YNSWYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEK 660
           YN+WYGLGM YLRQEKFEF++H F++A QINPRSSV+M Y G +LH  +R+++A+MMMEK
Sbjct: 602 YNAWYGLGMTYLRQEKFEFAQHQFQLALQINPRSSVIMCYYGIALHESKRNDEALMMMEK 661

Query: 661 AILADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHE 720
           A+L D KNPLP Y KA+IL SL  + +A +VLEELKE AP+ESSV+A +GKIY +   ++
Sbjct: 662 AVLTDAKNPLPKYYKAHILTSLGDYHKAQKVLEELKECAPQESSVHASLGKIYNQLKQYD 717

Query: 721 KAMLHFGLALDLKPSAADVATIKAAIEKLHVPDEI--EDNL 757
           KA+LHFG+ALDL PS +D   IKA +E+L +PDE+  E+NL
Sbjct: 722 KAVLHFGIALDLSPSPSDAVKIKAYMERLILPDELVTEENL 717

BLAST of Spg022062 vs. ExPASy Swiss-Prot
Match: Q4V8A2 (Cell division cycle protein 27 homolog OS=Rattus norvegicus OX=10116 GN=Cdc27 PE=2 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 4.2e-104
Identity = 260/792 (32.83%), Postives = 419/792 (52.90%), Query Frame = 0

Query: 9   VHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKGTQMA--QS 68
           +  +L+H+ +R+A+F+ ERL +E  SE  + LLA C+  + +AY AY +LKG      Q 
Sbjct: 12  IWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQC 71

Query: 69  RYLFAISCFQMDLLHEAEAALC--------PPNEPGAEIPNGAAGHY-LLGLIYRYTDRR 128
           +YL A  C  +  L E E  L           ++   E  + A     LLG +Y  TDR 
Sbjct: 72  KYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDLVTEFGDSACFTLSLLGHVYCKTDRL 131

Query: 129 RSAIQHFQQALSLDPLMWCAYEELCILGAAEDA------------SSVFGEAAVLCIQKQ 188
               + +Q++LSL+P +W  +E LC +G   D             SS         +   
Sbjct: 132 AKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKLTSLQNFSSCLPNTCTALVSNH 191

Query: 189 YLHHRSEN---LQTLSD--ELNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNL 248
            L HR       +T  D  ELN  +  +++S       S  +  ++    P N       
Sbjct: 192 SLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDNVPLGTGP 251

Query: 249 GGAASQIVN----GGSNISFYNTPSPVAAQLSAI---APPPLCRNTQQNGSSLNS-LGAD 308
              + Q+ N    G S +      SP+      +    P P   +  QN ++  S +   
Sbjct: 252 SILSKQVQNKPKTGRSLLGGPTALSPLTPSFGILPLETPSPGDGSYLQNYTNTPSVIDVP 311

Query: 309 SCSRSTVNSIIQ----APRRKFVDEGKLRKISGRLFS---DSGPRRSSR---LAGEAGAN 368
           S    T  S+ +      +  F   G  R+ +  L +    SGP+ S+    L+    + 
Sbjct: 312 STGAPTKKSVARMGHTGAKSVFSQSGNSREATPVLVAQTQSSGPQTSTTPQVLSPTITSP 371

Query: 369 TNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVV-RKGQSFANENIDEGIQNEAFDESR 428
            N     ++   T++S+     SK   + F   +  RK +S  N+            +  
Sbjct: 372 PNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKG--------GITQPN 431

Query: 429 SNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAKIINGASEILGLLRILGEGYRLSCL 488
            N SL I+      D+  + +G   ++   +     +   A+ ++ LLR +G+GY   C 
Sbjct: 432 INDSLEITKL----DSSIISEGKISTITPQI-QAFNLQKAAAGLMSLLREMGKGYLALCS 491

Query: 489 FRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLEADRAFSLARHASPHSLEGMDV 548
           + C++A+++   LP  HYSTGWVL Q+G+ YFEL +Y++A+R FS  R      +EGM++
Sbjct: 492 YNCKEAINILSHLPSHHYSTGWVLCQIGRAYFELSEYMQAERIFSEVRRIESFRVEGMEI 551

Query: 549 YSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMGNCYSLQKDHETALKNFQRAVQLNP 608
           YST L+HL++D+ LS L+++L   D+ +P++WCA GNC+SLQ++H+ A+K FQRA+Q++P
Sbjct: 552 YSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDP 611

Query: 609 RFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNSWYGLGMIYLRQEKFEFSEHHF 668
            +AYA+TL GHE+V  E+ +  +  +++A+RV+ RHYN+WYGLGMIY +QEKF  +E HF
Sbjct: 612 NYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHF 671

Query: 669 RMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAILADKKNPLPMYQKANILVSLER 728
           + A  INP+SSV++ ++G   HAL++SE A+  + KAI+ D KNPL  + +A++L + E+
Sbjct: 672 QKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEK 731

Query: 729 FDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAMLHFGLALDLKPSAADVATIKA 754
           +  ALQ LEELK+  P+ES VY L+GK+YK+      A+++F  A+DL P  A+   IK 
Sbjct: 732 YKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGAN-NQIKE 789

BLAST of Spg022062 vs. ExPASy Swiss-Prot
Match: A7Z061 (Cell division cycle protein 27 homolog OS=Bos taurus OX=9913 GN=CDC27 PE=2 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 5.5e-104
Identity = 262/798 (32.83%), Postives = 426/798 (53.38%), Query Frame = 0

Query: 9   VHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKGTQMA--QS 68
           +  +L+H+ +R+A+F+ ERL +E  SE  + LLA C+  + +AY AY +LKG      Q 
Sbjct: 12  IWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQC 71

Query: 69  RYLFAISCFQMDLLHEAEAALC--------PPNEPGAEIPNGAAGHY-LLGLIYRYTDRR 128
           +YL A  C  +  L E E  L           ++   E  + A     LLG +Y  TDR 
Sbjct: 72  KYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRL 131

Query: 129 RSAIQHFQQALSLDPLMWCAYEELCILGAAEDA------------SSVFGEAAVLCIQKQ 188
               + +Q++LSL+P +W  +E LC +G   D             SS    +    +   
Sbjct: 132 AKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKLTSLQNFSSCLPNSCTTLVSNH 191

Query: 189 YLHHRSENLQTLSDELNSASARNNSSDDVRTRQSKHTQSN-NLRDIPTNYHGAVNLGGAA 248
            L HR           ++      + +   ++ S +T S+ +  D        V LG   
Sbjct: 192 SLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGT 251

Query: 249 S----QIVN----GGSNISFYNTPSPVAAQLSAI---APPPLCRNTQQNGSSLNSL---- 308
           S    Q+ N    G S +      SP+      +    P P   +  QN ++ +S+    
Sbjct: 252 SILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTSSVIDVP 311

Query: 309 GADSCSRSTVNSIIQAPRRK-FVDEGKLRKISGRLFS---DSGPRRSSR---LAGEAGAN 368
              + S+ +V  I Q   +  F   G  R+++  L +    SGP+ S+    L+    + 
Sbjct: 312 PTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILVAQTQSSGPQTSTTPQVLSPTITSP 371

Query: 369 TNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVV-RKGQSFANE------NIDEGIQNE 428
            N     ++   T++S+     SK   + F   +  RK +S  N+      NI++ ++  
Sbjct: 372 PNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEIT 431

Query: 429 AFDESRSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAKIINGASEILGLLRILGEG 488
             D S  +    IS+ +P      L++ A                 A  ++ LLR +G+G
Sbjct: 432 KLDSSIISEG-KISTITPQIQAFNLQKAA-----------------AEGLMSLLREMGKG 491

Query: 489 YRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLEADRAFSLARHASPHS 548
           Y   C + C++A+++   LP  HY+TGWVL Q+G+ YFEL +Y++A+R FS  R    + 
Sbjct: 492 YLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYR 551

Query: 549 LEGMDVYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMGNCYSLQKDHETALKNFQR 608
           +EGM++YST L+HL++D+ LS L+++L   D+ +P++WCA GNC+SLQ++H+ A+K FQR
Sbjct: 552 VEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQR 611

Query: 609 AVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNSWYGLGMIYLRQEKFE 668
           A+Q++P +AYA+TL GHE+V  E+ +  +  +++A+RV+ RHYN+WYGLGMIY +QEKF 
Sbjct: 612 AIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFS 671

Query: 669 FSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAILADKKNPLPMYQKANI 728
            +E HF+ A  INP+SSV++ ++G   HAL++SE A+  + KAI+ D KNPL  + +A++
Sbjct: 672 LAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASV 731

Query: 729 LVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAMLHFGLALDLKPSAAD 754
           L + E++  ALQ LEELK+  P+ES VY L+GK+YK+      A+++F  A+DL P  A+
Sbjct: 732 LFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGAN 790

BLAST of Spg022062 vs. ExPASy Swiss-Prot
Match: A2A6Q5 (Cell division cycle protein 27 homolog OS=Mus musculus OX=10090 GN=Cdc27 PE=1 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 5.5e-104
Identity = 260/793 (32.79%), Postives = 419/793 (52.84%), Query Frame = 0

Query: 9   VHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKGTQMA--QS 68
           +  +L+H+ +R+A+F+ ERL +E  SE  + LLA C+  + +AY AY +LKG      Q 
Sbjct: 12  IWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQC 71

Query: 69  RYLFAISCFQMDLLHEAEAALC--------PPNEPGAEIPNGAAGHY-LLGLIYRYTDRR 128
           +YL A  C  +  L E E  L           ++   E  + A     LLG +Y  TDR 
Sbjct: 72  KYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDLVTEFGDSACFTLSLLGHVYCKTDRL 131

Query: 129 RSAIQHFQQALSLDPLMWCAYEELCILGAAEDA------------SSVFGEAAVLCIQKQ 188
               + +Q++LSL+P +W  +E LC +G   D             SS         +   
Sbjct: 132 AKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKLTSLQNFSSCLPNTCTTLVSNH 191

Query: 189 YLHHRSEN---LQTLSD--ELNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNL 248
            L HR       +T  D  ELN  +  +++S       S  +  ++    P N       
Sbjct: 192 SLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSTVISPDNVPLGPGT 251

Query: 249 GGAASQIVN----GGSNISFYNTPSPVAAQLSAI---APPPLCRNTQQNGSSLNSL---- 308
              + Q+ N    G S +      SP+      +    P P   +  QN ++  S+    
Sbjct: 252 AILSKQVQNKPKTGRSLLGGPTALSPLTPSFGILPLETPSPGDGSYLQNYTNTPSVIDVA 311

Query: 309 --GADSCSRSTVNSIIQAPRRKFVDEGKLRKISGRLFS---DSGPRRSSR---LAGEAGA 368
             GA +  +S         +  F   G  R+++  L +    SGP+ S+    L+    +
Sbjct: 312 PTGAPT-KKSVARMGQTGTKSVFSQSGNSREVTPVLVAQTQSSGPQTSTTPQVLSPTITS 371

Query: 369 NTNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVV-RKGQSFANENIDEGIQNEAFDES 428
             N     ++   T++S+     SK   + F   +  RK +S  N+    G+   + ++S
Sbjct: 372 PPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKG---GLTQPSINDS 431

Query: 429 RSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAKIINGASEILGLLRILGEGYRLSC 488
                L         D+  + +G   +V   +         A  ++ LLR +G+GY   C
Sbjct: 432 LEITKL---------DSSIISEGKITTVTPQIQAFNLQKAAAEGLMSLLREMGKGYLALC 491

Query: 489 LFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLEADRAFSLARHASPHSLEGMD 548
            + C++A+++   LP  HYSTGWVL Q+G+ YFEL +Y++A+R FS  R      +EGM+
Sbjct: 492 SYNCKEAINILSHLPSHHYSTGWVLCQIGRAYFELSEYMQAERIFSEVRRIESFRVEGME 551

Query: 549 VYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMGNCYSLQKDHETALKNFQRAVQLN 608
           +YST L+HL++D+ LS L+++L   D+ +P++WCA GNC+SLQ++H+ A+K FQRA+Q++
Sbjct: 552 IYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVD 611

Query: 609 PRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNSWYGLGMIYLRQEKFEFSEHH 668
           P +AYA+TL GHE+V  E+ +  +  +++A+RV+ RHYN+WYGLGMIY +QEKF  +E H
Sbjct: 612 PNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMH 671

Query: 669 FRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAILADKKNPLPMYQKANILVSLE 728
           F+ A  INP+SSV++ ++G   HAL++SE A+  + KAI+ D KNPL  + +A++L + E
Sbjct: 672 FQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANE 731

Query: 729 RFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAMLHFGLALDLKPSAADVATIK 754
           ++  ALQ LEELK+  P+ES VY L+GK+YK+      A+++F  A+DL P  A+   IK
Sbjct: 732 KYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGAN-NQIK 790

BLAST of Spg022062 vs. ExPASy TrEMBL
Match: A0A6J1F041 (cell division cycle protein 27 homolog B-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111440953 PE=4 SV=1)

HSP 1 Score: 1419.4 bits (3673), Expect = 0.0e+00
Identity = 717/756 (94.84%), Postives = 739/756 (97.75%), Query Frame = 0

Query: 1   MEAILTDCVHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60
           MEA LTDCVHHSL HFM+RNAIFMCERLC+EFPSETNMQLLAGCFLHNNQAYAAY ILKG
Sbjct: 1   MEATLTDCVHHSLRHFMYRNAIFMCERLCAEFPSETNMQLLAGCFLHNNQAYAAYRILKG 60

Query: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120
           TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA
Sbjct: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120

Query: 121 IQHFQQALSLDPLMWCAYEELCILGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTLSDE 180
           IQHFQ ALSLDPLMWCAYEELC+LGAAEDASSVFGEAAVLCIQKQYLHHRSENLQT +D+
Sbjct: 121 IQHFQLALSLDPLMWCAYEELCVLGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTSNDD 180

Query: 181 LNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAASQIVNGGSNISFYNTP 240
           +NSAS+RN++SDDVRTRQSKHTQSNNLRDIPTNYHGAVN+GGA SQ +NGGSNISFYNTP
Sbjct: 181 MNSASSRNSNSDDVRTRQSKHTQSNNLRDIPTNYHGAVNMGGAGSQSINGGSNISFYNTP 240

Query: 241 SPVAAQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTVNSIIQAPRRKFVDEGKLRKIS 300
           SPVAAQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRST+NSIIQAPRRKFVDEGKLRKIS
Sbjct: 241 SPVAAQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTINSIIQAPRRKFVDEGKLRKIS 300

Query: 301 GRLFSDSGPRRSSRLAGEAGANTNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVVRKG 360
           GRLFSDSGPRRSSRLAGEAGANTNVSAA AANNGTTNSTKYLG SK+NSI FRS+ VRKG
Sbjct: 301 GRLFSDSGPRRSSRLAGEAGANTNVSAAGAANNGTTNSTKYLGSSKMNSITFRSVAVRKG 360

Query: 361 QSFANENIDEGIQNEAFDESRSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAKIIN 420
           QSFANEN+DEGIQNEAFD+SRSNASLSISSSSPS+DNRTLEQGA KSVGGSLT+DAKIIN
Sbjct: 361 QSFANENVDEGIQNEAFDDSRSNASLSISSSSPSSDNRTLEQGAPKSVGGSLTSDAKIIN 420

Query: 421 GASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLE 480
           GASEIL LLRILGEGYRLSCLFRCQDALDVYLKLPYKHY+TGWVLSQVGKVYFELVDYLE
Sbjct: 421 GASEILCLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYNTGWVLSQVGKVYFELVDYLE 480

Query: 481 ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMGNCY 540
           ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELIS DRLAPQSWCAMGNCY
Sbjct: 481 ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISTDRLAPQSWCAMGNCY 540

Query: 541 SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNS 600
           SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNS
Sbjct: 541 SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNS 600

Query: 601 WYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAIL 660
           WYGLGMIYL+QEK EFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAIL
Sbjct: 601 WYGLGMIYLKQEKLEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAIL 660

Query: 661 ADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAM 720
           ADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMG IYKRRYMH+KAM
Sbjct: 661 ADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGNIYKRRYMHDKAM 720

Query: 721 LHFGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL 757
           LHFG+ALDLKPSAADVATIKAAIEKL VPDEIEDNL
Sbjct: 721 LHFGIALDLKPSAADVATIKAAIEKLLVPDEIEDNL 756

BLAST of Spg022062 vs. ExPASy TrEMBL
Match: A0A6J1H989 (cell division cycle protein 27 homolog B-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461662 PE=4 SV=1)

HSP 1 Score: 1416.7 bits (3666), Expect = 0.0e+00
Identity = 721/756 (95.37%), Postives = 738/756 (97.62%), Query Frame = 0

Query: 1   MEAILTDCVHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60
           ME IL DCVHHSL HFM+RNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG
Sbjct: 1   MEVILADCVHHSLRHFMYRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60

Query: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120
           TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA
Sbjct: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120

Query: 121 IQHFQQALSLDPLMWCAYEELCILGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTLSDE 180
           IQHFQQALS+DPLMWCAYEELCILGAAEDASSVFGEAAVL IQKQYLHHRSENLQTL+D+
Sbjct: 121 IQHFQQALSIDPLMWCAYEELCILGAAEDASSVFGEAAVLSIQKQYLHHRSENLQTLNDD 180

Query: 181 LNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAASQIVNGGSNISFYNTP 240
           LNSASARNN+SD+VR RQ KHTQSNNLRDIPTNY G VNLGGAASQIVNGGSNISFYNTP
Sbjct: 181 LNSASARNNNSDEVRIRQLKHTQSNNLRDIPTNYQGTVNLGGAASQIVNGGSNISFYNTP 240

Query: 241 SPVAAQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTVNSIIQAPRRKFVDEGKLRKIS 300
           SPVAAQLS+IAPPPLCRNTQQNGSS+NSLGAD+CSRSTVNSIIQAPRRKFVDEGKLRKIS
Sbjct: 241 SPVAAQLSSIAPPPLCRNTQQNGSSINSLGADNCSRSTVNSIIQAPRRKFVDEGKLRKIS 300

Query: 301 GRLFSDSGPRRSSRLAGEAGANTNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVVRKG 360
           GRLFSDSGPRRSSRLAGEA ANTNVS   AANNGTTNSTKYLGGSKLNSI  RSM VRKG
Sbjct: 301 GRLFSDSGPRRSSRLAGEAVANTNVS---AANNGTTNSTKYLGGSKLNSITLRSMAVRKG 360

Query: 361 QSFANENIDEGIQNEAFDESRSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAKIIN 420
           QSFANENIDEGIQNEAFD+SRSNASLS+SSSSPS+ NRTLEQGATKSVGGSLTNDAKIIN
Sbjct: 361 QSFANENIDEGIQNEAFDDSRSNASLSVSSSSPSSGNRTLEQGATKSVGGSLTNDAKIIN 420

Query: 421 GASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLE 480
           GASEILGLLRILGEG+RLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLE
Sbjct: 421 GASEILGLLRILGEGHRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLE 480

Query: 481 ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMGNCY 540
           ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELIS DRLAPQSWCAMGNCY
Sbjct: 481 ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISTDRLAPQSWCAMGNCY 540

Query: 541 SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNS 600
           SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGI+SYQSALRVDSRHYNS
Sbjct: 541 SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIQSYQSALRVDSRHYNS 600

Query: 601 WYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAIL 660
           WYGLGMIYLRQEK EFSEHHFRMAFQINPRSSVVMSYLGTSLHAL+RSEDAMMMMEKAIL
Sbjct: 601 WYGLGMIYLRQEKLEFSEHHFRMAFQINPRSSVVMSYLGTSLHALKRSEDAMMMMEKAIL 660

Query: 661 ADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAM 720
           ADKKNPLPMYQKANIL+SLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAM
Sbjct: 661 ADKKNPLPMYQKANILISLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAM 720

Query: 721 LHFGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL 757
           L+FGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL
Sbjct: 721 LNFGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL 753

BLAST of Spg022062 vs. ExPASy TrEMBL
Match: A0A6J1EZE5 (cell division cycle protein 27 homolog B-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111440953 PE=4 SV=1)

HSP 1 Score: 1414.1 bits (3659), Expect = 0.0e+00
Identity = 717/759 (94.47%), Postives = 739/759 (97.36%), Query Frame = 0

Query: 1   MEAILTDCVHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60
           MEA LTDCVHHSL HFM+RNAIFMCERLC+EFPSETNMQLLAGCFLHNNQAYAAY ILKG
Sbjct: 1   MEATLTDCVHHSLRHFMYRNAIFMCERLCAEFPSETNMQLLAGCFLHNNQAYAAYRILKG 60

Query: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120
           TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA
Sbjct: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120

Query: 121 IQHFQQALSLDPLMWCAYEELCILGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTLSDE 180
           IQHFQ ALSLDPLMWCAYEELC+LGAAEDASSVFGEAAVLCIQKQYLHHRSENLQT +D+
Sbjct: 121 IQHFQLALSLDPLMWCAYEELCVLGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTSNDD 180

Query: 181 LNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAASQIVNGGSNISFYNTP 240
           +NSAS+RN++SDDVRTRQSKHTQSNNLRDIPTNYHGAVN+GGA SQ +NGGSNISFYNTP
Sbjct: 181 MNSASSRNSNSDDVRTRQSKHTQSNNLRDIPTNYHGAVNMGGAGSQSINGGSNISFYNTP 240

Query: 241 SPVAA---QLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTVNSIIQAPRRKFVDEGKLR 300
           SPVAA   QLSAIAPPPLCRNTQQNGSSLNSLGADSCSRST+NSIIQAPRRKFVDEGKLR
Sbjct: 241 SPVAAQNFQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTINSIIQAPRRKFVDEGKLR 300

Query: 301 KISGRLFSDSGPRRSSRLAGEAGANTNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVV 360
           KISGRLFSDSGPRRSSRLAGEAGANTNVSAA AANNGTTNSTKYLG SK+NSI FRS+ V
Sbjct: 301 KISGRLFSDSGPRRSSRLAGEAGANTNVSAAGAANNGTTNSTKYLGSSKMNSITFRSVAV 360

Query: 361 RKGQSFANENIDEGIQNEAFDESRSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAK 420
           RKGQSFANEN+DEGIQNEAFD+SRSNASLSISSSSPS+DNRTLEQGA KSVGGSLT+DAK
Sbjct: 361 RKGQSFANENVDEGIQNEAFDDSRSNASLSISSSSPSSDNRTLEQGAPKSVGGSLTSDAK 420

Query: 421 IINGASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVD 480
           IINGASEIL LLRILGEGYRLSCLFRCQDALDVYLKLPYKHY+TGWVLSQVGKVYFELVD
Sbjct: 421 IINGASEILCLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYNTGWVLSQVGKVYFELVD 480

Query: 481 YLEADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMG 540
           YLEADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELIS DRLAPQSWCAMG
Sbjct: 481 YLEADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISTDRLAPQSWCAMG 540

Query: 541 NCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRH 600
           NCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRH
Sbjct: 541 NCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRH 600

Query: 601 YNSWYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEK 660
           YNSWYGLGMIYL+QEK EFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEK
Sbjct: 601 YNSWYGLGMIYLKQEKLEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEK 660

Query: 661 AILADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHE 720
           AILADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMG IYKRRYMH+
Sbjct: 661 AILADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGNIYKRRYMHD 720

Query: 721 KAMLHFGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL 757
           KAMLHFG+ALDLKPSAADVATIKAAIEKL VPDEIEDNL
Sbjct: 721 KAMLHFGIALDLKPSAADVATIKAAIEKLLVPDEIEDNL 759

BLAST of Spg022062 vs. ExPASy TrEMBL
Match: A0A6J1KZL3 (cell division cycle protein 27 homolog B-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498504 PE=4 SV=1)

HSP 1 Score: 1411.7 bits (3653), Expect = 0.0e+00
Identity = 713/756 (94.31%), Postives = 737/756 (97.49%), Query Frame = 0

Query: 1   MEAILTDCVHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60
           MEA LTDCVHHSL H M+RNAIFMCERLC+EFPSETNMQLLAGCFLHNNQAYAAY ILKG
Sbjct: 1   MEATLTDCVHHSLRHLMYRNAIFMCERLCAEFPSETNMQLLAGCFLHNNQAYAAYRILKG 60

Query: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120
           TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGA+GHYLLGLIYRYTDRRRSA
Sbjct: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGASGHYLLGLIYRYTDRRRSA 120

Query: 121 IQHFQQALSLDPLMWCAYEELCILGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTLSDE 180
           IQHFQQALSLDPLMWCAYEELC+LGAAEDASSVFGEAAVLCIQKQYL+HRSENLQT +D+
Sbjct: 121 IQHFQQALSLDPLMWCAYEELCVLGAAEDASSVFGEAAVLCIQKQYLNHRSENLQTSNDD 180

Query: 181 LNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAASQIVNGGSNISFYNTP 240
           +NSAS+RN++SDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGA SQ VNGGSNISFYNTP
Sbjct: 181 MNSASSRNSNSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAGSQTVNGGSNISFYNTP 240

Query: 241 SPVAAQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTVNSIIQAPRRKFVDEGKLRKIS 300
           SPVAAQLSAIAPPPLCRNTQQNGSSLNSLG+DSCSRST+NSIIQAPRRKFVDEGKLRKIS
Sbjct: 241 SPVAAQLSAIAPPPLCRNTQQNGSSLNSLGSDSCSRSTINSIIQAPRRKFVDEGKLRKIS 300

Query: 301 GRLFSDSGPRRSSRLAGEAGANTNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVVRKG 360
           GRLFSDSGPRRSSRLAGEAG NTNVSAA AANNG TNSTKYLG SK+NSI FRS+ VRKG
Sbjct: 301 GRLFSDSGPRRSSRLAGEAGGNTNVSAAGAANNGATNSTKYLGSSKMNSITFRSVAVRKG 360

Query: 361 QSFANENIDEGIQNEAFDESRSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAKIIN 420
           QSFANEN+DEGIQNEA+D+SRSNASLSISSSSPS+DNRTLEQGA KSVGGSLT+DAKIIN
Sbjct: 361 QSFANENVDEGIQNEAYDDSRSNASLSISSSSPSSDNRTLEQGAPKSVGGSLTSDAKIIN 420

Query: 421 GASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVDYLE 480
           GASEIL LLRILGEGYRLSCLFRCQDALDVYLKLPYKHY+TGWVLSQVGKVYFELVDYLE
Sbjct: 421 GASEILCLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYNTGWVLSQVGKVYFELVDYLE 480

Query: 481 ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMGNCY 540
           ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELIS DRLAPQSWCAMGNCY
Sbjct: 481 ADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISTDRLAPQSWCAMGNCY 540

Query: 541 SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNS 600
           SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNS
Sbjct: 541 SLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRHYNS 600

Query: 601 WYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAIL 660
           WYGLGMIYL+QEK EFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAIL
Sbjct: 601 WYGLGMIYLKQEKLEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEKAIL 660

Query: 661 ADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHEKAM 720
           ADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMG IYKRRYMH+KAM
Sbjct: 661 ADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGNIYKRRYMHDKAM 720

Query: 721 LHFGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL 757
           LHFG+ALDLKPSAADVATIKAAIEKL VPDEIEDNL
Sbjct: 721 LHFGIALDLKPSAADVATIKAAIEKLLVPDEIEDNL 756

BLAST of Spg022062 vs. ExPASy TrEMBL
Match: A0A6J1HAT9 (cell division cycle protein 27 homolog B-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461662 PE=4 SV=1)

HSP 1 Score: 1411.4 bits (3652), Expect = 0.0e+00
Identity = 721/759 (94.99%), Postives = 738/759 (97.23%), Query Frame = 0

Query: 1   MEAILTDCVHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60
           ME IL DCVHHSL HFM+RNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG
Sbjct: 1   MEVILADCVHHSLRHFMYRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60

Query: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120
           TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA
Sbjct: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120

Query: 121 IQHFQQALSLDPLMWCAYEELCILGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTLSDE 180
           IQHFQQALS+DPLMWCAYEELCILGAAEDASSVFGEAAVL IQKQYLHHRSENLQTL+D+
Sbjct: 121 IQHFQQALSIDPLMWCAYEELCILGAAEDASSVFGEAAVLSIQKQYLHHRSENLQTLNDD 180

Query: 181 LNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAASQIVNGGSNISFYNTP 240
           LNSASARNN+SD+VR RQ KHTQSNNLRDIPTNY G VNLGGAASQIVNGGSNISFYNTP
Sbjct: 181 LNSASARNNNSDEVRIRQLKHTQSNNLRDIPTNYQGTVNLGGAASQIVNGGSNISFYNTP 240

Query: 241 SPVAA---QLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTVNSIIQAPRRKFVDEGKLR 300
           SPVAA   QLS+IAPPPLCRNTQQNGSS+NSLGAD+CSRSTVNSIIQAPRRKFVDEGKLR
Sbjct: 241 SPVAAQNLQLSSIAPPPLCRNTQQNGSSINSLGADNCSRSTVNSIIQAPRRKFVDEGKLR 300

Query: 301 KISGRLFSDSGPRRSSRLAGEAGANTNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVV 360
           KISGRLFSDSGPRRSSRLAGEA ANTNVS   AANNGTTNSTKYLGGSKLNSI  RSM V
Sbjct: 301 KISGRLFSDSGPRRSSRLAGEAVANTNVS---AANNGTTNSTKYLGGSKLNSITLRSMAV 360

Query: 361 RKGQSFANENIDEGIQNEAFDESRSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAK 420
           RKGQSFANENIDEGIQNEAFD+SRSNASLS+SSSSPS+ NRTLEQGATKSVGGSLTNDAK
Sbjct: 361 RKGQSFANENIDEGIQNEAFDDSRSNASLSVSSSSPSSGNRTLEQGATKSVGGSLTNDAK 420

Query: 421 IINGASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVD 480
           IINGASEILGLLRILGEG+RLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVD
Sbjct: 421 IINGASEILGLLRILGEGHRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVD 480

Query: 481 YLEADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMG 540
           YLEADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELIS DRLAPQSWCAMG
Sbjct: 481 YLEADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISTDRLAPQSWCAMG 540

Query: 541 NCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRH 600
           NCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGI+SYQSALRVDSRH
Sbjct: 541 NCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIQSYQSALRVDSRH 600

Query: 601 YNSWYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEK 660
           YNSWYGLGMIYLRQEK EFSEHHFRMAFQINPRSSVVMSYLGTSLHAL+RSEDAMMMMEK
Sbjct: 601 YNSWYGLGMIYLRQEKLEFSEHHFRMAFQINPRSSVVMSYLGTSLHALKRSEDAMMMMEK 660

Query: 661 AILADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHE 720
           AILADKKNPLPMYQKANIL+SLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHE
Sbjct: 661 AILADKKNPLPMYQKANILISLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHE 720

Query: 721 KAMLHFGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL 757
           KAML+FGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL
Sbjct: 721 KAMLNFGLALDLKPSAADVATIKAAIEKLHVPDEIEDNL 756

BLAST of Spg022062 vs. TAIR 10
Match: AT2G20000.1 (CDC27 family protein )

HSP 1 Score: 1072.4 bits (2772), Expect = 1.6e-313
Identity = 550/758 (72.56%), Postives = 641/758 (84.56%), Query Frame = 0

Query: 1   MEAILTDCVHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60
           MEA+L DCV++SL HF+++NAIFMCERLC+EFPSE N+QLLA  +L NNQAY+AYH+LKG
Sbjct: 1   MEAMLVDCVNNSLRHFVYKNAIFMCERLCAEFPSEVNLQLLATSYLQNNQAYSAYHLLKG 60

Query: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120
           TQMAQSRYLFA+SCFQMDLL+EAE+ALCP NEPGAEIPNGAAGHYLLGLIY+YTDRR++A
Sbjct: 61  TQMAQSRYLFALSCFQMDLLNEAESALCPVNEPGAEIPNGAAGHYLLGLIYKYTDRRKNA 120

Query: 121 IQHFQQALSLDPLMWCAYEELCILGAAEDASSVFGEAAVLCIQKQYLHHRSEN--LQTLS 180
            Q F+Q+L++DPL+W AYEELCILGAAE+A++VFGE A L IQKQY+   S +  L T +
Sbjct: 121 AQQFKQSLTIDPLLWAAYEELCILGAAEEATAVFGETAALSIQKQYMQQLSTSLGLNTYN 180

Query: 181 DELNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAASQIVNGG-SNISFY 240
           +E NS S +N SS+D   RQSKHTQS+ L+DI  N+H         S  VNGG SN+SFY
Sbjct: 181 EERNSTSTKNTSSEDYSPRQSKHTQSHGLKDISGNFH---------SHGVNGGVSNMSFY 240

Query: 241 NTPSPVAAQLSAIAPPPLCRNTQQNGSSLNSLGADSCSRSTVNSIIQAPRRKFVDEGKLR 300
           NTPSPVAAQLS IAPPPL RN Q   ++ NSL  DS  +STVNS +QAPRRKFVDEGKLR
Sbjct: 241 NTPSPVAAQLSGIAPPPLFRNFQPAVANPNSLITDSSPKSTVNSTLQAPRRKFVDEGKLR 300

Query: 301 KISGRLFSDSGPRRSSRLAGEAGANTNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVV 360
           KISGRLFSDSGPRRSSRL+ ++GAN N S A  + N   N++KYLGGSKL+S+A RS+ +
Sbjct: 301 KISGRLFSDSGPRRSSRLSADSGANINSSVATVSGN-VNNASKYLGGSKLSSLALRSVTL 360

Query: 361 RKGQSFANENIDEGIQNEAFDESRSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAK 420
           RKG S+ANEN+DEG++ E FD+SR N + S + S  SND    ++  T S+GG   +   
Sbjct: 361 RKGHSWANENMDEGVRGEPFDDSRPNTA-STTGSMASND----QEDETMSIGGIAMSSQT 420

Query: 421 IINGASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVD 480
           I  G SEIL LLR LGEG RLS ++RCQ+ALD Y+KLP+KHY+TGWVLSQVGK YFEL+D
Sbjct: 421 ITIGVSEILNLLRTLGEGCRLSYMYRCQEALDTYMKLPHKHYNTGWVLSQVGKAYFELID 480

Query: 481 YLEADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMG 540
           YLEA++AF LAR ASP+ LEGMD+YSTVLYHLKEDMKLSYLAQELIS DRLAPQSWCAMG
Sbjct: 481 YLEAEKAFRLARLASPYCLEGMDIYSTVLYHLKEDMKLSYLAQELISTDRLAPQSWCAMG 540

Query: 541 NCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRH 600
           NCYSLQKDHETALKNF RAVQLNPRFAYAHTLCGHEY  LEDFENG+KSYQ+ALRVD+RH
Sbjct: 541 NCYSLQKDHETALKNFLRAVQLNPRFAYAHTLCGHEYTTLEDFENGMKSYQNALRVDTRH 600

Query: 601 YNSWYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEK 660
           YN+WYGLGMIYLRQEK EFSEHHFRMAF INP SSV+MSYLGTSLHAL+RSE+A+ +ME+
Sbjct: 601 YNAWYGLGMIYLRQEKLEFSEHHFRMAFLINPSSSVIMSYLGTSLHALKRSEEALEIMEQ 660

Query: 661 AILADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHE 720
           AI+AD+KNPLPMYQKANILV LER DEAL+VLEELKEYAP ESSVYALMG+IYKRR MH+
Sbjct: 661 AIVADRKNPLPMYQKANILVCLERLDEALEVLEELKEYAPSESSVYALMGRIYKRRNMHD 720

Query: 721 KAMLHFGLALDLKPSAADVATIKAAIEKLHVPDEIEDN 756
           KAMLHFGLALD+KP A DVA IKAA+EKLHVPDEI+++
Sbjct: 721 KAMLHFGLALDMKPPATDVAAIKAAMEKLHVPDEIDES 743

BLAST of Spg022062 vs. TAIR 10
Match: AT3G16320.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 643.7 bits (1659), Expect = 1.9e-184
Identity = 363/761 (47.70%), Postives = 491/761 (64.52%), Query Frame = 0

Query: 1   MEAILTDCVHHSLHHFMHRNAIFMCERLCSEFPSETNMQLLAGCFLHNNQAYAAYHILKG 60
           ME +L +CV  +L+HFM  NAIF+CE L ++FPSE N+QLLA C+L N+QAY+AY+ILKG
Sbjct: 2   MENLLANCVQKNLNHFMFTNAIFLCELLLAQFPSEVNLQLLARCYLSNSQAYSAYYILKG 61

Query: 61  TQMAQSRYLFAISCFQMDLLHEAEAALCPPNEPGAEIPNGAAGHYLLGLIYRYTDRRRSA 120
           ++  QSRYLFA SCF++DLL EAEAAL P  +   E+P GAAGHYLLGLIYRY+ R+  +
Sbjct: 62  SKTPQSRYLFAFSCFKLDLLGEAEAALLPCEDYAEEVPGGAAGHYLLGLIYRYSGRKNCS 121

Query: 121 IQHFQQALSLDPLMWCAYEELCILGAAEDASSVFGEAAVLCIQKQYLHHRSENLQTLSDE 180
           IQ F+ ALS DPL W AY ELC LGAAE+AS+VFG  A   +QK  +  R    +  +  
Sbjct: 122 IQQFRMALSFDPLCWEAYGELCSLGAAEEASTVFGNVASQRLQKTCVEQRISFSEGAT-- 181

Query: 181 LNSASARNNSSDDVRTRQSKHTQSNNLRDIPTNYHGAVNLGGAASQIVNGGSNISFYNTP 240
           ++  +  + +  D    Q++H    N +D+               Q+   G ++   NTP
Sbjct: 182 IDQITDSDKALKDTGLSQTEHIPGENQQDLKIMQQPGDIPPNTDRQLSTNGWDL---NTP 241

Query: 241 SPVAAQLSAIAPPPLCRNTQQ---NGSSLNSLGADSCSRSTVNSIIQAPRRKFVDEGKLR 300
           SPV  Q+    PP L +N ++    GS ++  G            ++  RR F  E    
Sbjct: 242 SPVLLQVMDALPPLLLKNMRRPAVEGSLMSVHG------------VRVRRRNFFSE---- 301

Query: 301 KISGRLFSDSGPRRSSRLAGEAGANTNVSAAAAANNGTTNSTKYLGGSKLNSIAFRSMVV 360
           ++S     +SG RRS+R+A             + + G  +   +L  S+ N     S ++
Sbjct: 302 ELSAEAQEESGRRRSARIAARK------KNPMSQSFGKDSHWLHLSPSESNYAPSLSSMI 361

Query: 361 RKGQSFANENIDEGIQNEAFDESRSNASLSISSSSPSNDNRTLEQGATKSVGGSLTNDAK 420
            K +      I    +     +S S+   S+     SN +             S  +   
Sbjct: 362 GKCR------IQSSKEATTSGQSVSDIGSSVDDEEKSNPSE------------SSPDRFS 421

Query: 421 IINGASEILGLLRILGEGYRLSCLFRCQDALDVYLKLPYKHYSTGWVLSQVGKVYFELVD 480
           +I+G SE+L LL+ILG+G+R   +++CQ+AL  Y KL  K Y+T WVL QVGK YFEL D
Sbjct: 422 LISGISEVLSLLKILGDGHRHLHMYKCQEALLAYQKLSQKQYNTHWVLMQVGKAYFELQD 481

Query: 481 YLEADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQELISADRLAPQSWCAMG 540
           Y  AD +F+LA    P++LEGMD YSTVLYHLKE+M+L YLAQELIS DRL+P+SWCA+G
Sbjct: 482 YFNADSSFTLAHQKYPYALEGMDTYSTVLYHLKEEMRLGYLAQELISVDRLSPESWCAVG 541

Query: 541 NCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDSRH 600
           NCYSL+KDH+TALK FQRA+QLN RF YAHTLCGHE+ ALE+FE+  + Y+ AL +D+RH
Sbjct: 542 NCYSLRKDHDTALKMFQRAIQLNERFTYAHTLCGHEFAALEEFEDAERCYRKALGIDTRH 601

Query: 601 YNSWYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMMMEK 660
           YN+WYGLGM YLRQEKFEF++H F++A QINPRSSV+M Y G +LH  +R+++A+MMMEK
Sbjct: 602 YNAWYGLGMTYLRQEKFEFAQHQFQLALQINPRSSVIMCYYGIALHESKRNDEALMMMEK 661

Query: 661 AILADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRYMHE 720
           A+L D KNPLP Y KA+IL SL  + +A +VLEELKE AP+ESSV+A +GKIY +   ++
Sbjct: 662 AVLTDAKNPLPKYYKAHILTSLGDYHKAQKVLEELKECAPQESSVHASLGKIYNQLKQYD 717

Query: 721 KAMLHFGLALDLKPSAADVATIKAAIEKLHVPDEI--EDNL 757
           KA+LHFG+ALDL PS +D   IKA +E+L +PDE+  E+NL
Sbjct: 722 KAVLHFGIALDLSPSPSDAVKIKAYMERLILPDELVTEENL 717

BLAST of Spg022062 vs. TAIR 10
Match: AT3G48150.1 (anaphase-promoting complex subunit 8 )

HSP 1 Score: 120.6 bits (301), Expect = 5.6e-27
Identity = 71/236 (30.08%), Postives = 121/236 (51.27%), Query Frame = 0

Query: 461 TGWVLSQVGKVYFELVDYLEADRAFSLARHASPHSLEGMDVYSTVLYHLKEDMKLSYLAQ 520
           + ++ +Q  K  + L ++ + +  F       P+ +E MD+YS VLY  +    LSYLA 
Sbjct: 270 SNYIQAQTAKAQYSLREFDQVEIMFEELLRNDPYRVEDMDLYSNVLYAKEACAALSYLAH 329

Query: 521 ELISADRLAPQSWCAMGNCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDF 580
           ++   D+  P+S C +GN YSL+  HE A+  F+RA++LN ++  A TL GHEYV +++ 
Sbjct: 330 KVFLTDKYRPESCCIIGNYYSLKGQHEKAVMYFRRALKLNKKYLSAWTLMGHEYVEMKNT 389

Query: 581 ENGIKSYQSALRVDSRHYNSWYGLGMIYLRQEKFEFSEHHFRMAFQINPRSS-----VVM 640
              I +Y+ A+ ++   Y +WYGLG  Y       ++ H+FR +    P  S     +  
Sbjct: 390 PAAIDAYRRAVDINPTDYRAWYGLGQAYEMMGMPFYALHYFRKSIFFLPNDSRLWIAMAK 449

Query: 641 SYLGTSLHALQRSEDAMMMMEKAILADKKNPLPMYQKANILVSLERFDEALQVLEE 692
            Y    L+ L   E+A+   ++A+       + + Q A +   L R +EA    E+
Sbjct: 450 CYQTEQLYML---EEAIKCYKRAVNCTDTEGIALNQLAKLHQKLGRNEEAAYYFEK 502

BLAST of Spg022062 vs. TAIR 10
Match: AT4G37460.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 54.3 bits (129), Expect = 4.9e-07
Identity = 48/256 (18.75%), Postives = 105/256 (41.02%), Query Frame = 0

Query: 477 DYLEADRAFSLARHASPHSLEGMDVYSTVLYHLKED--MKLSYLAQELISADRLAPQSWC 536
           D    ++ +++AR +  HS+      S  +  + E    K   +  +++  +   P++  
Sbjct: 276 DESRKNKKYTIARISGTHSISVDFRLSRGIAQVNEGNYTKAISIFDKVLKEEPTYPEALI 335

Query: 537 AMGNCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVD 596
             G  Y+ Q++ E+A+ +F +A+Q NP  + A    G    AL ++   ++    AL  +
Sbjct: 336 GRGTAYAFQRELESAIADFTKAIQSNPAASEAWKRRGQARAALGEYVEAVEDLTKALVFE 395

Query: 597 SRHYNSWYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSEDAMMM 656
               +  +  G++  + + F  +     +  +    +    +YLG +  +L   + A   
Sbjct: 396 PNSPDVLHERGIVNFKSKDFTAAVKDLSICLKQEKDNKSAYTYLGLAFASLGEYKKAEEA 455

Query: 657 MEKAILADKKNPLPMYQKANILVSLERFDEALQVLEELKEYAPRESSVYALMGKIYKRRY 716
             K+I  D          A     L    +AL+ +E++ +   R    Y L G ++    
Sbjct: 456 HLKSIQLDSNYLEAWLHLAQFYQELADHCKALECIEQVLQVDNRVWKAYHLRGLVFHGLG 515

Query: 717 MHEKAMLHFGLALDLK 731
            H KA+    + L ++
Sbjct: 516 EHRKAIQELSIGLSIE 531

BLAST of Spg022062 vs. TAIR 10
Match: AT5G56290.1 (peroxin 5 )

HSP 1 Score: 52.4 bits (124), Expect = 1.9e-06
Identity = 29/114 (25.44%), Postives = 52/114 (45.61%), Query Frame = 0

Query: 536 MGNCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENGIKSYQSALRVDS 595
           +G  Y+L ++ + A+ +FQ A+QL P         G          + I +YQ AL +  
Sbjct: 596 LGVLYNLSREFDRAITSFQTALQLKPNDYSLWNKLGATQANSVQSADAISAYQQALDLKP 655

Query: 596 RHYNSWYGLGMIYLRQEKFEFSEHHFRMAFQINPRSSVVMSYLGTSLHALQRSE 650
            +  +W  +G+ Y  Q  ++ S  ++  A  +NP++     YL  SL    R +
Sbjct: 656 NYVRAWANMGISYANQGMYKESIPYYVRALAMNPKADNAWQYLRLSLSCASRQD 709