Spg021918 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg021918
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Cytochrome P450
Location	scaffold2: 2981515 .. 2982828 (+)
RNA-Seq Expression	Spg021918
Synteny	Spg021918

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACAGCTACACGTTCTATCTGGCGTGCCGACTGTTCGTGAGCTTGGAGGATCAAAACGAGGTGCAGAGGATCTTCAAGAGGTTCGAAGAGATCGCGTCGGGGATCATCTCGGTGCCGATAGACTTTCCGGGAACGATGTTCAGGAAAGCCATTAGGGCGTCGGAGTTTGTGAGGAAGGAGCTGTCGAAGATCATAAAGCAGAGGAAGATAGATCTGGCGGAGGGGAAGGCCTCGCCGACACAAGACATTCTGTCGCACATGCTTTTGACCAGCGACGGCAACGGCCAATTCATCCACGAAGAAGACATTGCCTCTAAAATTGTCGGTCTCCTCGTCGGCGGCCACGACACCGGCGTCTGCACCTGCACTTTCATCGTCAAGTACTTGGCTACTCTCCCCCACGTCTACGAAAATGTCTACAATGGTAAGTAATTAATTTGCTTTTTTTTTTTTAAATTAAAAAAAGTATATGTTTTTTTAATTTAGGTTAAATAATTTATTAATGGTTGATATTGGGAAATAATTTTAGAGCAAATGGAGATAGCAAAATCCAAGGGAGCAGGAGAGCTATTGACTTGGGAAGATCTGCAGAAGATGAAGTATTCTTGGAATGTAGCTTGTGAAGTGCTCAGGCTCTTTCCTCCTGTTCAAGGAGCTTTCAGAGTTGCCCTCACAGACTTTGTCTTCAATGGCTTCAGTATCCCCAAGGGCTGGAAGGTAATTCAAAAACTCATTACACGCTTTAAAATTTAAGTGAGCATAGGTCAACGTTAATTGACATTGTACCCTCGACCAATTTGTAACATTTTCTTTTTTCCGTTTTGGGTGTGAAGTTGTATTGGAGTGCGGCGGCAACCCACAAAAACCCGGAGTACTTTCCAGATCCGTACAAGTTCGATCCATCGAGGTTCGAAGGGAAAGGTCCGGCTCCGTTCACGTACGTGCCGTTCGGGGGCGGGCCCAGAATGTGCCCCGGCAAAGAGTATGCAAGGCTAGAGGTGCTAGTGTTTATGCACCATTTGGTCAAGCGCTTCAAAATTGAACTTTGCGATCCTGATGAAAAAGTGACTTGCAATCCAATTCCCATGCCTGTCCACGGCCTCCCCGTCCGCCTCATTCCCCATGCCTAACTCCATCATCATCTGCTTTAAGGTTTCATTGGACTTGTATGCAGATAAAATCTCCCAGTTATAACTCAAAACTTGTTATGTTTTTTTTCTTTTTTTATTGTGTATTAGAATGCTATTAGATTCCCATTCCATGAGAATGAATAATGTTTTGGGTTTGTTTTATGGAATCATACTTAAAATT

mRNA sequence

ATGCACAGCTACACGTTCTATCTGGCGTGCCGACTGTTCGTGAGCTTGGAGGATCAAAACGAGGTGCAGAGGATCTTCAAGAGGTTCGAAGAGATCGCGTCGGGGATCATCTCGGTGCCGATAGACTTTCCGGGAACGATGTTCAGGAAAGCCATTAGGGCGTCGGAGTTTGTGAGGAAGGAGCTGTCGAAGATCATAAAGCAGAGGAAGATAGATCTGGCGGAGGGGAAGGCCTCGCCGACACAAGACATTCTGTCGCACATGCTTTTGACCAGCGACGGCAACGGCCAATTCATCCACGAAGAAGACATTGCCTCTAAAATTGTCGGTCTCCTCGTCGGCGGCCACGACACCGGCGTCTGCACCTGCACTTTCATCGTCAAGTACTTGGCTACTCTCCCCCACGTCTACGAAAATGTCTACAATGAGCAAATGGAGATAGCAAAATCCAAGGGAGCAGGAGAGCTATTGACTTGGGAAGATCTGCAGAAGATGAAGTATTCTTGGAATGTAGCTTGTGAAGTGCTCAGGCTCTTTCCTCCTGTTCAAGGAGCTTTCAGAGTTGCCCTCACAGACTTTGTCTTCAATGGCTTCAGTATCCCCAAGGGCTGGAAGTTGTATTGGAGTGCGGCGGCAACCCACAAAAACCCGGAGTACTTTCCAGATCCGTACAAGTTCGATCCATCGAGGTTCGAAGGGAAAGGTCCGGCTCCGTTCACGTACGTGCCGTTCGGGGGCGGGCCCAGAATGTGCCCCGGCAAAGAGTATGCAAGGCTAGAGGTGCTAGTGTTTATGCACCATTTGGTCAAGCGCTTCAAAATTGAACTTTGCGATCCTGATGAAAAAGTGACTTGCAATCCAATTCCCATGCCTGTCCACGGCCTCCCCGTCCGCCTCATTCCCCATGCCTAA

Coding sequence (CDS)

ATGCACAGCTACACGTTCTATCTGGCGTGCCGACTGTTCGTGAGCTTGGAGGATCAAAACGAGGTGCAGAGGATCTTCAAGAGGTTCGAAGAGATCGCGTCGGGGATCATCTCGGTGCCGATAGACTTTCCGGGAACGATGTTCAGGAAAGCCATTAGGGCGTCGGAGTTTGTGAGGAAGGAGCTGTCGAAGATCATAAAGCAGAGGAAGATAGATCTGGCGGAGGGGAAGGCCTCGCCGACACAAGACATTCTGTCGCACATGCTTTTGACCAGCGACGGCAACGGCCAATTCATCCACGAAGAAGACATTGCCTCTAAAATTGTCGGTCTCCTCGTCGGCGGCCACGACACCGGCGTCTGCACCTGCACTTTCATCGTCAAGTACTTGGCTACTCTCCCCCACGTCTACGAAAATGTCTACAATGAGCAAATGGAGATAGCAAAATCCAAGGGAGCAGGAGAGCTATTGACTTGGGAAGATCTGCAGAAGATGAAGTATTCTTGGAATGTAGCTTGTGAAGTGCTCAGGCTCTTTCCTCCTGTTCAAGGAGCTTTCAGAGTTGCCCTCACAGACTTTGTCTTCAATGGCTTCAGTATCCCCAAGGGCTGGAAGTTGTATTGGAGTGCGGCGGCAACCCACAAAAACCCGGAGTACTTTCCAGATCCGTACAAGTTCGATCCATCGAGGTTCGAAGGGAAAGGTCCGGCTCCGTTCACGTACGTGCCGTTCGGGGGCGGGCCCAGAATGTGCCCCGGCAAAGAGTATGCAAGGCTAGAGGTGCTAGTGTTTATGCACCATTTGGTCAAGCGCTTCAAAATTGAACTTTGCGATCCTGATGAAAAAGTGACTTGCAATCCAATTCCCATGCCTGTCCACGGCCTCCCCGTCCGCCTCATTCCCCATGCCTAA

Protein sequence

MHSYTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKELSKIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCTCTFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPVQGAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYVPFGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIPHA

Homology

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BLAST of Spg021918 vs. NCBI nr
Match: XP_038878724.1 (beta-amyrin 28-monooxygenase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 555.1 bits (1429), Expect = 3.8e-154
Identity = 261/300 (87.00%), Postives = 280/300 (93.33%), Query Frame = 0


Query: 4   YTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKELS 63
           YTFYLACRLF+S+ED NEV+RIFKRFEEIASGIISVPIDFPGTMFRKAI+ASEFVRKEL 
Sbjct: 180 YTFYLACRLFMSMEDVNEVERIFKRFEEIASGIISVPIDFPGTMFRKAIKASEFVRKELL 239

Query: 64  KIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCTC 123
           +II+QRK DL+EGKAS TQDILSHMLLTSD  GQFIHEEDIASKI+GLLVGGHDTGVCTC
Sbjct: 240 RIIRQRKTDLSEGKASATQDILSHMLLTSDSTGQFIHEEDIASKILGLLVGGHDTGVCTC 299

Query: 124 TFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPVQ 183
           TF+VKYLA LPHVYENVYNEQMEIA+SK AGELLTWEDLQKMKYSWNV CEVLRLFPPVQ
Sbjct: 300 TFVVKYLAALPHVYENVYNEQMEIARSKAAGELLTWEDLQKMKYSWNVVCEVLRLFPPVQ 359

Query: 184 GAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYVP 243
           GAFRVALTDF FNGF IPKGWKLYWSAAATHKN +YF +P KF+P RFEGKGPAPFTYVP
Sbjct: 360 GAFRVALTDFAFNGFYIPKGWKLYWSAAATHKNEKYFAEPLKFEPRRFEGKGPAPFTYVP 419

Query: 244 FGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIPHA 303
           FGGGPRMCPGKEYARLEVLVFMHHLV+RFK++LC P+EK+T NPIP P+HGLPVRL P A
Sbjct: 420 FGGGPRMCPGKEYARLEVLVFMHHLVRRFKLQLCIPNEKITANPIPTPLHGLPVRLFPLA 479

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BLAST of Spg021918 vs. NCBI nr
Match: XP_038878723.1 (beta-amyrin 28-monooxygenase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 549.7 bits (1415), Expect = 1.6e-152
Identity = 261/303 (86.14%), Postives = 280/303 (92.41%), Query Frame = 0


Query: 4   YTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKELS 63
           YTFYLACRLF+S+ED NEV+RIFKRFEEIASGIISVPIDFPGTMFRKAI+ASEFVRKEL 
Sbjct: 180 YTFYLACRLFMSMEDVNEVERIFKRFEEIASGIISVPIDFPGTMFRKAIKASEFVRKELL 239

Query: 64  KIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCTC 123
           +II+QRK DL+EGKAS TQDILSHMLLTSD  GQFIHEEDIASKI+GLLVGGHDTGVCTC
Sbjct: 240 RIIRQRKTDLSEGKASATQDILSHMLLTSDSTGQFIHEEDIASKILGLLVGGHDTGVCTC 299

Query: 124 TFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPVQ 183
           TF+VKYLA LPHVYENVYNEQMEIA+SK AGELLTWEDLQKMKYSWNV CEVLRLFPPVQ
Sbjct: 300 TFVVKYLAALPHVYENVYNEQMEIARSKAAGELLTWEDLQKMKYSWNVVCEVLRLFPPVQ 359

Query: 184 GAFRVALTDFVFNGFSIPKGW---KLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFT 243
           GAFRVALTDF FNGF IPKGW   KLYWSAAATHKN +YF +P KF+P RFEGKGPAPFT
Sbjct: 360 GAFRVALTDFAFNGFYIPKGWKDVKLYWSAAATHKNEKYFAEPLKFEPRRFEGKGPAPFT 419

Query: 244 YVPFGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLI 303
           YVPFGGGPRMCPGKEYARLEVLVFMHHLV+RFK++LC P+EK+T NPIP P+HGLPVRL 
Sbjct: 420 YVPFGGGPRMCPGKEYARLEVLVFMHHLVRRFKLQLCIPNEKITANPIPTPLHGLPVRLF 479

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BLAST of Spg021918 vs. NCBI nr
Match: AMZ03377.1 (cytochrome P450 CYP716A69 [Plectranthus barbatus])

HSP 1 Score: 490.3 bits (1261), Expect = 1.2e-134
Identity = 227/300 (75.67%), Postives = 259/300 (86.33%), Query Frame = 0


Query: 3   SYTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKEL 62
           +YTF+LACRLFVS+ED  +V +  + F  +ASG+IS+PID PGT F K I+AS FVRKEL
Sbjct: 175 NYTFWLACRLFVSIEDPAQVDKFAEPFNLLASGLISIPIDLPGTPFNKGIKASNFVRKEL 234

Query: 63  SKIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCT 122
             IIKQR++DLAEGKASPTQDILSHMLLTSD NG+F+ E DIA K++GLL+GGHDT    
Sbjct: 235 VSIIKQRRVDLAEGKASPTQDILSHMLLTSDENGKFMQELDIADKVLGLLIGGHDTASSA 294

Query: 123 CTFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPV 182
           CTFIVKYLA LPHVYE VYNEQMEIAKSK AGELL WEDLQKMKYSWNVACEVLRL  P+
Sbjct: 295 CTFIVKYLAELPHVYERVYNEQMEIAKSKAAGELLNWEDLQKMKYSWNVACEVLRLASPL 354

Query: 183 QGAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYV 242
           QGAFR A+TDFV+NGFSIPKGWKLYWSA +THKNPE+FP+P KFDPSRFEG GPAP+T+V
Sbjct: 355 QGAFREAITDFVYNGFSIPKGWKLYWSANSTHKNPEFFPEPTKFDPSRFEGSGPAPYTFV 414

Query: 243 PFGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIPH 302
           PFGGGPRMCPGKEYARLE+LVFMHHLVKRF+ E+  PDEK+  +P+P+P  GLPVRL PH
Sbjct: 415 PFGGGPRMCPGKEYARLEILVFMHHLVKRFRWEMAIPDEKIVVDPMPIPAKGLPVRLFPH 474

[Close]

BLAST of Spg021918 vs. NCBI nr
Match: XP_040999159.1 (beta-amyrin 28-monooxygenase-like [Juglans microcarpa x Juglans regia])

HSP 1 Score: 487.6 bits (1254), Expect = 7.5e-134
Identity = 227/300 (75.67%), Postives = 259/300 (86.33%), Query Frame = 0


Query: 3   SYTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKEL 62
           SYTF+LA RLF+S+ED N + +  + F+ +ASG+ISVPID PGT F +AI+AS+F+RKEL
Sbjct: 179 SYTFWLASRLFISVEDPNHIAKFSEPFQVLASGLISVPIDLPGTPFNRAIKASDFIRKEL 238

Query: 63  SKIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCT 122
            KIIKQRK+DLAEGKA+PTQDILSHMLLT D NGQ++ E DIA KI+GLL+GGHDT    
Sbjct: 239 HKIIKQRKVDLAEGKATPTQDILSHMLLTCDENGQYMGELDIADKILGLLIGGHDTASAA 298

Query: 123 CTFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPV 182
           CTFIVKYL+ LP +YE VYNEQMEIAKSK  GELL W+DLQKMKYSWNVACEVLRL PP+
Sbjct: 299 CTFIVKYLSELPDIYEGVYNEQMEIAKSKAPGELLNWDDLQKMKYSWNVACEVLRLAPPL 358

Query: 183 QGAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYV 242
           QGAFR ALTDFVFNGFSIPKGWKLYWSA +THK+ EYFP+P KFDPSRFEGKGPAP+T+V
Sbjct: 359 QGAFREALTDFVFNGFSIPKGWKLYWSANSTHKSAEYFPEPQKFDPSRFEGKGPAPYTFV 418

Query: 243 PFGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIPH 302
           PFGGGPRMCPGKEYARLE+LVFMHHLVKRFK E   PDEK+  +P+PMP  GLPVRL PH
Sbjct: 419 PFGGGPRMCPGKEYARLEILVFMHHLVKRFKWEKMIPDEKIIVDPMPMPAKGLPVRLFPH 478

[Close]

BLAST of Spg021918 vs. NCBI nr
Match: XP_018818694.1 (beta-amyrin 28-monooxygenase-like [Juglans regia] >KAF5469395.1 hypothetical protein F2P56_013475 [Juglans regia])

HSP 1 Score: 487.3 bits (1253), Expect = 9.8e-134
Identity = 224/299 (74.92%), Postives = 256/299 (85.62%), Query Frame = 0


Query: 4   YTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKELS 63
           YTF+LACRLF+S+ED N +      F+ +ASGIIS+PID PGT F +AI+AS F+RKEL 
Sbjct: 180 YTFWLACRLFISVEDPNHIATFADPFQALASGIISIPIDLPGTPFNRAIKASNFIRKELV 239

Query: 64  KIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCTC 123
           KIIKQRKIDLAEGKASPTQDILSHMLLT D NGQ++ E DIA KI+GLL+GGHDT    C
Sbjct: 240 KIIKQRKIDLAEGKASPTQDILSHMLLTCDENGQYMAELDIADKILGLLIGGHDTASAAC 299

Query: 124 TFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPVQ 183
           TFIVKYLA +P VYE VYNEQMEIAKSK AGE+L W+D+QKM+YSWNVACEV+RL PP+Q
Sbjct: 300 TFIVKYLAEIPDVYEGVYNEQMEIAKSKAAGEMLNWDDIQKMRYSWNVACEVMRLAPPLQ 359

Query: 184 GAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYVP 243
           G FR ALTDF FNGFSIPKGWKLYWSA +THKNP+YFP+P KFDPSRFEG+GPAP+TYVP
Sbjct: 360 GGFREALTDFAFNGFSIPKGWKLYWSANSTHKNPDYFPEPQKFDPSRFEGRGPAPYTYVP 419

Query: 244 FGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIPH 303
           FGGGPRMCPGKEYARLE+LVFMHHLVKRFK +   PDEK+  +P+P+P  GLPVRL PH
Sbjct: 420 FGGGPRMCPGKEYARLEILVFMHHLVKRFKWDKLIPDEKIVVDPMPIPAKGLPVRLFPH 478

[Close]

BLAST of Spg021918 vs. ExPASy Swiss-Prot
Match: A0A3Q7HBJ5 (Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A44 PE=1 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 3.6e-131
Identity = 216/299 (72.24%), Postives = 251/299 (83.95%), Query Frame = 0


Query: 4   YTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKELS 63
           YTF+LACRLF+S+ED   V +    F+ +ASG+IS+PID PGT F +AI+AS F+RKEL 
Sbjct: 174 YTFWLACRLFLSVEDPKHVAKFADPFDVLASGLISIPIDLPGTPFNRAIKASNFIRKELV 233

Query: 64  KIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCTC 123
           +IIKQRKIDL EGK S TQDILSHMLLT D NG+F+ + DIA KI+GLL+GGHDT    C
Sbjct: 234 RIIKQRKIDLGEGKVSSTQDILSHMLLTCDENGKFLGDLDIADKILGLLIGGHDTASSAC 293

Query: 124 TFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPVQ 183
           +FIVKYLA LPH+Y+ VY EQMEIAKSKG GELL WED+QKMKYSWNVACEVLRL PP+Q
Sbjct: 294 SFIVKYLAELPHIYQRVYTEQMEIAKSKGPGELLRWEDIQKMKYSWNVACEVLRLAPPLQ 353

Query: 184 GAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYVP 243
           GAFR AL+DF+FNGF IPKGWK+YWSA +THK  E+FPDP KFDPSRFEG GPAP+T+VP
Sbjct: 354 GAFREALSDFMFNGFYIPKGWKIYWSANSTHKREEFFPDPEKFDPSRFEGSGPAPYTFVP 413

Query: 244 FGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIPH 303
           FGGGPRMCPGKEYARLE+LVFMHHLVKRFK E   P EK+  NP+P+P +GLP+RL PH
Sbjct: 414 FGGGPRMCPGKEYARLEILVFMHHLVKRFKFEKIIPHEKIIVNPMPIPANGLPLRLYPH 472

[Close]

BLAST of Spg021918 vs. ExPASy Swiss-Prot
Match: K4CEE8 (Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 1.4e-130
Identity = 217/299 (72.58%), Postives = 251/299 (83.95%), Query Frame = 0


Query: 4   YTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKELS 63
           YTF+LACRLFVS+ED N V R+   F+ +ASG+IS+PI+ PGT F +AI+AS F+RKEL 
Sbjct: 180 YTFWLACRLFVSVEDPNHVARLADPFDVLASGLISIPINLPGTPFNRAIKASNFIRKELV 239

Query: 64  KIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCTC 123
            IIKQRKIDL EGKAS +QDILSHMLLTSD NG+F+HE DIA KI+GLL+GGHDT    C
Sbjct: 240 AIIKQRKIDLTEGKASDSQDILSHMLLTSDENGKFMHELDIADKILGLLIGGHDTASSAC 299

Query: 124 TFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPVQ 183
            FIVKYLA LP +Y+ VY EQ+EIAKSKG GELL+WED++KMKYSWNVACEVLRL PP+Q
Sbjct: 300 AFIVKYLAELPEIYDQVYKEQIEIAKSKGPGELLSWEDIKKMKYSWNVACEVLRLAPPLQ 359

Query: 184 GAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYVP 243
           GAFR AL DF FNGFSIPKGWK+YWSA +TH NPE F DP KFDPSRFEG GPAP+T+VP
Sbjct: 360 GAFREALVDFTFNGFSIPKGWKIYWSANSTHINPEVFVDPLKFDPSRFEGNGPAPYTFVP 419

Query: 244 FGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIPH 303
           FGGGPRMCPGKEYARLE+LVFMHHLVKRF  +    DEK+  NP+P+P +GLP+RL PH
Sbjct: 420 FGGGPRMCPGKEYARLEILVFMHHLVKRFSWKKIIRDEKIVVNPMPIPANGLPIRLYPH 478

[Close]

BLAST of Spg021918 vs. ExPASy Swiss-Prot
Match: A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 2.0e-129
Identity = 215/300 (71.67%), Postives = 251/300 (83.67%), Query Frame = 0


Query: 3   SYTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKEL 62
           +YTF++AC++FVS+E+  +V ++ + F  IASGIISVPID PGT F  AI++S+ VR +L
Sbjct: 180 NYTFWIACKVFVSVEEPTQVAKLLEPFNAIASGIISVPIDLPGTPFNSAIKSSKIVRDKL 239

Query: 63  SKIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCT 122
             IIKQRK DL EGKASPTQDILSHMLLTSD NG+F+ E DIA KI+GLL+GGHDT    
Sbjct: 240 MGIIKQRKTDLGEGKASPTQDILSHMLLTSDENGKFMTEGDIADKILGLLIGGHDTASSA 299

Query: 123 CTFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPV 182
           CTF+VK+LA LP +YE VY EQMEIAKSK AGELL WED+QKMKYSWNVACEVLRL PP+
Sbjct: 300 CTFVVKFLAELPEIYEGVYKEQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPL 359

Query: 183 QGAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYV 242
           QGAFR ALTDF +NGFSIPKGWKLYWSA +TH+N E FP+P KFDPSRFEG GP P+++V
Sbjct: 360 QGAFREALTDFSYNGFSIPKGWKLYWSANSTHRNSEVFPEPLKFDPSRFEGAGPPPYSFV 419

Query: 243 PFGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIPH 302
           PFGGGPRMCPGKEYARLE+LVFMHH+VKRFK E   PDEK+  NP+P+P  GLPVRL PH
Sbjct: 420 PFGGGPRMCPGKEYARLEILVFMHHVVKRFKWEKVIPDEKIVVNPMPIPAKGLPVRLFPH 479

[Close]

BLAST of Spg021918 vs. ExPASy Swiss-Prot
Match: Q2MJ20 (Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 5.8e-129
Identity = 215/299 (71.91%), Postives = 251/299 (83.95%), Query Frame = 0


Query: 4   YTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKELS 63
           YTF LACRLF+S+ED+N V +    F+ IA+GIIS+PID PGT F KAI+AS F+RKEL 
Sbjct: 179 YTFLLACRLFMSVEDENHVAKFSDPFQLIAAGIISLPIDLPGTPFNKAIKASNFIRKELI 238

Query: 64  KIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCTC 123
           KIIKQR++DLAEG ASPTQDILSHMLLTSD NG+ ++E +IA KI+GLL+GGHDT    C
Sbjct: 239 KIIKQRRVDLAEGTASPTQDILSHMLLTSDENGKSMNELNIADKILGLLIGGHDTASVAC 298

Query: 124 TFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPVQ 183
           TF+VKYL  LPH+Y+ VY EQMEIAKSK AGELL W+DL+KMKYSWNVACEV+RL PP+Q
Sbjct: 299 TFLVKYLGELPHIYDKVYQEQMEIAKSKPAGELLNWDDLKKMKYSWNVACEVMRLSPPLQ 358

Query: 184 GAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYVP 243
           G FR A+TDF+FNGFSIPKGWKLYWSA +THKN E FP P KFDP+RFEG GPAP+T+VP
Sbjct: 359 GGFREAITDFMFNGFSIPKGWKLYWSANSTHKNAECFPMPEKFDPTRFEGNGPAPYTFVP 418

Query: 244 FGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIPH 303
           FGGGPRMCPGKEYARLE+LVFMH+LVKRFK E   PDEK+  +P P+P   LP+RL PH
Sbjct: 419 FGGGPRMCPGKEYARLEILVFMHNLVKRFKWEKVIPDEKIIVDPFPIPAKDLPIRLYPH 477

[Close]

BLAST of Spg021918 vs. ExPASy Swiss-Prot
Match: I7C6E8 (Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 8.4e-128
Identity = 214/300 (71.33%), Postives = 250/300 (83.33%), Query Frame = 0


Query: 3   SYTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKEL 62
           SYTF++AC++FVS+E+  +V  + + F  IASGIISVPID PGT F  AI++S+ VR++L
Sbjct: 180 SYTFWIACKVFVSVEEPAQVAELLEPFSAIASGIISVPIDLPGTPFNSAIKSSKIVRRKL 239

Query: 63  SKIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCT 122
             IIKQRKIDL EGKAS TQDILSHMLLTSD +G+F+ E DIA KI+GLL+GGHDT    
Sbjct: 240 VGIIKQRKIDLGEGKASATQDILSHMLLTSDESGKFMGEGDIADKILGLLIGGHDTASSA 299

Query: 123 CTFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPV 182
           CTF+VK+LA LP +YE VY EQMEI KSK AGELL WED+QKMKYSWNVACEVLRL PP+
Sbjct: 300 CTFVVKFLAELPQIYEGVYQEQMEIVKSKKAGELLKWEDIQKMKYSWNVACEVLRLAPPL 359

Query: 183 QGAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYV 242
           QGAFR AL+DF +NGFSIPKGWKLYWSA +TH N E FP+P KFDPSRF+G GP PF++V
Sbjct: 360 QGAFREALSDFTYNGFSIPKGWKLYWSANSTHINSEVFPEPLKFDPSRFDGAGPPPFSFV 419

Query: 243 PFGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIPH 302
           PFGGGPRMCPGKEYARLE+LVFMHHLVKRFK E   PDEK+  NP+P+P +GLPVRL PH
Sbjct: 420 PFGGGPRMCPGKEYARLEILVFMHHLVKRFKWEKVIPDEKIVVNPMPIPANGLPVRLFPH 479

[Close]

BLAST of Spg021918 vs. ExPASy TrEMBL
Match: A0A1B0VRK2 (Cytochrome P450 CYP716A69 OS=Plectranthus barbatus OX=41228 PE=2 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 5.6e-135
Identity = 227/300 (75.67%), Postives = 259/300 (86.33%), Query Frame = 0


Query: 3   SYTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKEL 62
           +YTF+LACRLFVS+ED  +V +  + F  +ASG+IS+PID PGT F K I+AS FVRKEL
Sbjct: 175 NYTFWLACRLFVSIEDPAQVDKFAEPFNLLASGLISIPIDLPGTPFNKGIKASNFVRKEL 234

Query: 63  SKIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCT 122
             IIKQR++DLAEGKASPTQDILSHMLLTSD NG+F+ E DIA K++GLL+GGHDT    
Sbjct: 235 VSIIKQRRVDLAEGKASPTQDILSHMLLTSDENGKFMQELDIADKVLGLLIGGHDTASSA 294

Query: 123 CTFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPV 182
           CTFIVKYLA LPHVYE VYNEQMEIAKSK AGELL WEDLQKMKYSWNVACEVLRL  P+
Sbjct: 295 CTFIVKYLAELPHVYERVYNEQMEIAKSKAAGELLNWEDLQKMKYSWNVACEVLRLASPL 354

Query: 183 QGAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYV 242
           QGAFR A+TDFV+NGFSIPKGWKLYWSA +THKNPE+FP+P KFDPSRFEG GPAP+T+V
Sbjct: 355 QGAFREAITDFVYNGFSIPKGWKLYWSANSTHKNPEFFPEPTKFDPSRFEGSGPAPYTFV 414

Query: 243 PFGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIPH 302
           PFGGGPRMCPGKEYARLE+LVFMHHLVKRF+ E+  PDEK+  +P+P+P  GLPVRL PH
Sbjct: 415 PFGGGPRMCPGKEYARLEILVFMHHLVKRFRWEMAIPDEKIVVDPMPIPAKGLPVRLFPH 474

[Close]

BLAST of Spg021918 vs. ExPASy TrEMBL
Match: A0A2I4EH28 (beta-amyrin 28-monooxygenase-like OS=Juglans regia OX=51240 GN=LOC108989520 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 4.8e-134
Identity = 224/299 (74.92%), Postives = 256/299 (85.62%), Query Frame = 0


Query: 4   YTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKELS 63
           YTF+LACRLF+S+ED N +      F+ +ASGIIS+PID PGT F +AI+AS F+RKEL 
Sbjct: 180 YTFWLACRLFISVEDPNHIATFADPFQALASGIISIPIDLPGTPFNRAIKASNFIRKELV 239

Query: 64  KIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCTC 123
           KIIKQRKIDLAEGKASPTQDILSHMLLT D NGQ++ E DIA KI+GLL+GGHDT    C
Sbjct: 240 KIIKQRKIDLAEGKASPTQDILSHMLLTCDENGQYMAELDIADKILGLLIGGHDTASAAC 299

Query: 124 TFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPVQ 183
           TFIVKYLA +P VYE VYNEQMEIAKSK AGE+L W+D+QKM+YSWNVACEV+RL PP+Q
Sbjct: 300 TFIVKYLAEIPDVYEGVYNEQMEIAKSKAAGEMLNWDDIQKMRYSWNVACEVMRLAPPLQ 359

Query: 184 GAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYVP 243
           G FR ALTDF FNGFSIPKGWKLYWSA +THKNP+YFP+P KFDPSRFEG+GPAP+TYVP
Sbjct: 360 GGFREALTDFAFNGFSIPKGWKLYWSANSTHKNPDYFPEPQKFDPSRFEGRGPAPYTYVP 419

Query: 244 FGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIPH 303
           FGGGPRMCPGKEYARLE+LVFMHHLVKRFK +   PDEK+  +P+P+P  GLPVRL PH
Sbjct: 420 FGGGPRMCPGKEYARLEILVFMHHLVKRFKWDKLIPDEKIVVDPMPIPAKGLPVRLFPH 478

[Close]

BLAST of Spg021918 vs. ExPASy TrEMBL
Match: A0A3G9ICU8 (Cytochrome P450 716A259 OS=Avicennia marina OX=82927 GN=CYP716A259 PE=2 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 6.2e-134
Identity = 225/300 (75.00%), Postives = 257/300 (85.67%), Query Frame = 0


Query: 3   SYTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKEL 62
           +YTF+LACRLFVS+ED  +V +  + F  +ASG+IS+PID PGT F K I+AS ++RKEL
Sbjct: 177 NYTFWLACRLFVSIEDPQQVDKFAEPFNLLASGLISIPIDLPGTPFNKGIKASNYIRKEL 236

Query: 63  SKIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCT 122
             IIKQRKIDLAEGKASPTQDILSHMLLTSD NG+F+HE D+A KI+GLL+GGHDT    
Sbjct: 237 VSIIKQRKIDLAEGKASPTQDILSHMLLTSDENGKFMHELDVADKILGLLIGGHDTASSA 296

Query: 123 CTFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPV 182
           CTFIVKYLA LPHVY+ VY EQMEIAKSKG GELL WEDLQKMKYSWNVACEVLRL PP+
Sbjct: 297 CTFIVKYLAELPHVYDRVYEEQMEIAKSKGPGELLNWEDLQKMKYSWNVACEVLRLAPPL 356

Query: 183 QGAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYV 242
           QGAFR A+TDF +NGFSIPKGWKLYWSA +THKNP+ FP+P KFDPSRFEG GPAP+T+V
Sbjct: 357 QGAFREAMTDFAYNGFSIPKGWKLYWSANSTHKNPQCFPEPQKFDPSRFEGSGPAPYTFV 416

Query: 243 PFGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIPH 302
           PFGGGPRMCPGK+YARLE+LVFM+HLVKRFK E   PDEK+  +P+PMP  GLPVRL PH
Sbjct: 417 PFGGGPRMCPGKQYARLEILVFMYHLVKRFKWEKIIPDEKIVVDPMPMPAKGLPVRLYPH 476

[Close]

BLAST of Spg021918 vs. ExPASy TrEMBL
Match: A0A1B0VRJ3 (Cytochrome P450 CYP716A67 OS=Plectranthus barbatus OX=41228 PE=2 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 8.1e-134
Identity = 225/300 (75.00%), Postives = 258/300 (86.00%), Query Frame = 0


Query: 3   SYTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKEL 62
           +YTF+LACRLFVS+ED N+V  + + F  +ASG+IS+PID PGT F K I+AS FVRKEL
Sbjct: 175 NYTFWLACRLFVSIEDPNQVDELAEPFNLLASGLISIPIDLPGTPFNKGIKASNFVRKEL 234

Query: 63  SKIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCT 122
             IIKQRK+DLAEGKASPTQDILSHMLLTSD NG+F+ E DIA KI+GLL+GGHDT    
Sbjct: 235 VSIIKQRKVDLAEGKASPTQDILSHMLLTSDENGKFMQELDIADKILGLLIGGHDTASSA 294

Query: 123 CTFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPV 182
           CTFIVKYLA LP VYE VYNEQMEIAKSK AGELL WEDLQKMKYSWNVACEVLR+  P+
Sbjct: 295 CTFIVKYLAELPQVYERVYNEQMEIAKSKAAGELLNWEDLQKMKYSWNVACEVLRMASPL 354

Query: 183 QGAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYV 242
           QGAFR A+TDFV+NGFSIPKGWKLYWSA +THKNPE+FP+P KFDPSRFEG GPAP+T+V
Sbjct: 355 QGAFREAITDFVYNGFSIPKGWKLYWSANSTHKNPEFFPEPRKFDPSRFEGSGPAPYTFV 414

Query: 243 PFGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIPH 302
           PFGGGPRMCPGKEYARLE+LVFMHHLVKRF+ E+  P+E +  +P+P+P  GLP+RL PH
Sbjct: 415 PFGGGPRMCPGKEYARLEILVFMHHLVKRFRWEMSIPNENIVVDPMPIPAKGLPIRLFPH 474

[Close]

BLAST of Spg021918 vs. ExPASy TrEMBL
Match: A0A1S4CJC5 (beta-amyrin 28-oxidase-like OS=Nicotiana tabacum OX=4097 GN=LOC107819508 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.5e-132
Identity = 223/299 (74.58%), Postives = 254/299 (84.95%), Query Frame = 0


Query: 4   YTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKELS 63
           YTF+LACRLFVS+ED N V ++   F+ +ASG+IS+PID PGT F +AI+AS F+RKEL 
Sbjct: 181 YTFWLACRLFVSVEDPNHVAKLADPFDVLASGLISIPIDLPGTPFNRAIKASNFIRKELV 240

Query: 64  KIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCTC 123
           +IIKQRKIDLAEGKA P QDILSHMLLTSD NG+F+HE DIA KI+GLL+GGHDT    C
Sbjct: 241 EIIKQRKIDLAEGKALPNQDILSHMLLTSDENGKFMHELDIADKILGLLIGGHDTASSAC 300

Query: 124 TFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPVQ 183
            FIVKYLA LP +YE VY EQMEIAKSK AGELL WED+QKM+YSWNVACEVLRL PP+Q
Sbjct: 301 AFIVKYLAELPEIYEGVYKEQMEIAKSKAAGELLNWEDIQKMRYSWNVACEVLRLAPPLQ 360

Query: 184 GAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYVP 243
           GAFR AL DF FNGFSIPKGWK+YWSA +TH+NPE FP+P KFDPSRFEG GPAP+T+VP
Sbjct: 361 GAFREALADFTFNGFSIPKGWKIYWSANSTHRNPEVFPEPLKFDPSRFEGSGPAPYTFVP 420

Query: 244 FGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIPH 303
           FGGGPRMCPGKEYARLE+LVFMHHLVKRFK E   PDEK+  NP+P+P  GLPV+L PH
Sbjct: 421 FGGGPRMCPGKEYARLEILVFMHHLVKRFKWEKIIPDEKIVVNPMPIPAKGLPVKLYPH 479

[Close]

BLAST of Spg021918 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 380.6 bits (976), Expect = 1.2e-105
Identity = 175/301 (58.14%), Postives = 224/301 (74.42%), Query Frame = 0


Query: 4   YTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRKELS 63
           +TF +ACR F+S+ED   V+++ ++F  +A GI S+PID PGT F +AI+AS  +RKE+S
Sbjct: 181 FTFSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVS 240

Query: 64  KIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCTC 123
            I++QRK +L  GKA    DILSHML+    N     +ED+A KI+GLL+GGHDT    C
Sbjct: 241 AIVRQRKEELKAGKALEEHDILSHMLM----NIGETKDEDLADKIIGLLIGGHDTASIVC 300

Query: 124 TFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPVQ 183
           TF+V YLA  PHVY+ V  EQ EI K K   E L WED++KM+YSWNVACEV+R+ PP+ 
Sbjct: 301 TFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLS 360

Query: 184 GAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYVP 243
           G FR A+  F F GF IPKGWKLYWSA ATH NP+YFP+P +F+P+RFEG GP P+TYVP
Sbjct: 361 GTFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVP 420

Query: 244 FGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDE-KVTCNPIPMPVHGLPVRLIPH 303
           FGGGPRMCPGKEYARLE+L+FMH+LV RFK E   P+E K+  +P+P+P  GLP+R+ P 
Sbjct: 421 FGGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFPQ 477

[Close]

BLAST of Spg021918 vs. TAIR 10
Match: AT5G36130.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 216.9 bits (551), Expect = 2.3e-56
Identity = 94/140 (67.14%), Postives = 113/140 (80.71%), Query Frame = 0


Query: 165 MKYSWNVACEVLRLFPPVQGAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPY 224
           M+YSWNVACEV+R+ PP+ G FR A+  F F GF IPKGWKLYWSA ATHKNPEYFP+P 
Sbjct: 1   MRYSWNVACEVMRIVPPLAGTFREAIDHFSFKGFYIPKGWKLYWSATATHKNPEYFPEPE 60

Query: 225 KFDPSRFEGKGPAPFTYVPFGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDE-KV 284
           KF+PSRFEG GP P+TYVPFGGG R+CPG+EYARLE+L+FMH+LVKRFK E   P E K+
Sbjct: 61  KFEPSRFEGSGPKPYTYVPFGGGSRICPGREYARLEILIFMHNLVKRFKWEKVFPKENKL 120

Query: 285 TCNPIPMPVHGLPVRLIPHA 304
             +P P+P  GLP+R+ P +
Sbjct: 121 VADPAPIPAKGLPIRIFPQS 140

[Close]

BLAST of Spg021918 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 214.9 bits (546), Expect = 8.8e-56
Identity = 107/274 (39.05%), Postives = 161/274 (58.76%), Query Frame = 0


Query: 29  FEEIASGIISVPIDFPGTMFRKAIRAS-EFVRKELSKIIKQRKIDLAEGKASPTQDILSH 88
           FE +  G+ ++P++FP + F +A +A  E     + K+ ++R+    EG   P   + S 
Sbjct: 212 FERVLEGVFALPVEFPCSKFARAKKARLEIETFLVGKVREKRREMEKEGAEKPNTTLFSR 271

Query: 89  MLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGVCTCTFIVKYLATLPHVYENVYNEQMEI 148
             L  +     I EE++   +V L+   HDT     +   K LA  P   + +  E  +I
Sbjct: 272 --LVEELIKGVITEEEVVDNMVLLVFAAHDTTSYAMSMTFKMLAQHPTCRDTLLQEHAQI 331

Query: 149 AKSKGAGELLTWEDLQKMKYSWNVACEVLRLFPPVQGAFRVALTDFVFNGFSIPKGWKLY 208
             +KG GE LT ED++KMKYSW V  E +RL PP+ G+FR A+ D  + G++IPKGWK+ 
Sbjct: 332 KANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKAVADIDYGGYTIPKGWKIL 391

Query: 209 WSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFTYVPFGGGPRMCPGKEYARLEVLVFMHH 268
           W+   TH NPE F DP  FDP+RF+ K    +TY+PFGGGPR+C G + A++ +LVFMH 
Sbjct: 392 WTTYGTHYNPEIFQDPMSFDPTRFD-KPIQAYTYLPFGGGPRLCAGHQLAKISILVFMHF 451

Query: 269 LVKRFKIELCDPDEKVTCNPIPMPVHGLPVRLIP 302
           +V  F   L  PDE ++ +P+P P  G+P+++ P
Sbjct: 452 VVTGFDWSLVYPDETISMDPLPFPSLGMPIKISP 482

[Close]

BLAST of Spg021918 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 195.3 bits (495), Expect = 7.2e-50
Identity = 111/299 (37.12%), Postives = 163/299 (54.52%), Query Frame = 0


Query: 1   MHSYTFYLACRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVRK 60
           M +YTF +A    +  ++    + + + +  +  G  S+PI+ PGT+F KA++A    RK
Sbjct: 171 MKTYTFNVALISILGKDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKA----RK 230

Query: 61  ELSKIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTGV 120
           EL++I+        +  +S T D+L   +    G    + +E IA  I+G++    DT  
Sbjct: 231 ELAQILANILSKRRQNPSSHT-DLLGSFMEDKAG----LTDEQIADNIIGVIFAARDTTA 290

Query: 121 CTCTFIVKYLATLPHVYENVYNEQMEIAKSKGAGELLTWEDLQKMKYSWNVACEVLRLFP 180
              T+I+KYLA  P V E V  EQM I K K  GE LTWED +KM  ++ V  E LR   
Sbjct: 291 SVLTWILKYLADNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAAT 350

Query: 181 PVQGAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAPFT 240
            +   FR A+ D  + G+ IPKGWK+       H N + F DP KFDPSRFE   P P T
Sbjct: 351 ILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFE-VAPKPNT 410

Query: 241 YVPFGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVRL 300
           ++PFG G   CPG E A+LE+ V +HHL  +++  +  P + +   P  +P +GLP+ L
Sbjct: 411 FMPFGSGIHSCPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIAL 459

[Close]

BLAST of Spg021918 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 194.9 bits (494), Expect = 9.4e-50
Identity = 107/303 (35.31%), Postives = 164/303 (54.13%), Query Frame = 0


Query: 1   MHSYTFYLA-CRLFVSLEDQNEVQRIFKRFEEIASGIISVPIDFPGTMFRKAIRASEFVR 60
           M  Y F +A    F   E+   +  I   ++ +  G  S+P+D PGT+F K+++A   + 
Sbjct: 185 MKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELS 244

Query: 61  KELSKIIKQRKIDLAEGKASPTQDILSHMLLTSDGNGQFIHEEDIASKIVGLLVGGHDTG 120
           +EL K+I++R+ +  E        +L  +L   D     + +  IA  I+G++    DT 
Sbjct: 245 EELRKVIEKRRENGRE-----EGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTT 304

Query: 121 VCTCTFIVKYLATLPHVYENVYNEQMEI-AKSKGAGELLTWEDLQKMKYSWNVACEVLRL 180
               T+++KYL   P++ + V  EQ  I  K K     ++WED +KM  +  V  E LR 
Sbjct: 305 ASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRA 364

Query: 181 FPPVQGAFRVALTDFVFNGFSIPKGWKLYWSAAATHKNPEYFPDPYKFDPSRFEGKGPAP 240
              +   FR A+ D  ++G+ IPKGWK+       H + E+FPDP KFDPSRFE   P P
Sbjct: 365 ASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKP 424

Query: 241 FTYVPFGGGPRMCPGKEYARLEVLVFMHHLVKRFKIELCDPDEKVTCNPIPMPVHGLPVR 300
           +TY+PFG G   CPG E A+LE+L+ +HHL   F+ E+   +E +   P P+P  GLP+R
Sbjct: 425 YTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIR 481

Query: 301 LIP 302
           + P
Sbjct: 485 VTP 481

The following BLAST results are available for this feature:

BLAST of Spg021918 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878724.1	3.8e-154	87.00	beta-amyrin 28-monooxygenase-like isoform X2 [Benincasa hispida]	[more]
XP_038878723.1	1.6e-152	86.14	beta-amyrin 28-monooxygenase-like isoform X1 [Benincasa hispida]	[more]
AMZ03377.1	1.2e-134	75.67	cytochrome P450 CYP716A69 [Plectranthus barbatus]	[more]
XP_040999159.1	7.5e-134	75.67	beta-amyrin 28-monooxygenase-like [Juglans microcarpa x Juglans regia]	[more]
XP_018818694.1	9.8e-134	74.92	beta-amyrin 28-monooxygenase-like [Juglans regia] >KAF5469395.1 hypothetical pro...	[more]

BLAST of Spg021918 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A3Q7HBJ5	3.6e-131	72.24	Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A44 PE=1 S...	[more]
K4CEE8	1.4e-130	72.58	Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 S...	[more]
A0A0S2II38	2.0e-129	71.67	Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=...	[more]
Q2MJ20	5.8e-129	71.91	Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV...	[more]
I7C6E8	8.4e-128	71.33	Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1	[more]

BLAST of Spg021918 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1B0VRK2	5.6e-135	75.67	Cytochrome P450 CYP716A69 OS=Plectranthus barbatus OX=41228 PE=2 SV=1	[more]
A0A2I4EH28	4.8e-134	74.92	beta-amyrin 28-monooxygenase-like OS=Juglans regia OX=51240 GN=LOC108989520 PE=3...	[more]
A0A3G9ICU8	6.2e-134	75.00	Cytochrome P450 716A259 OS=Avicennia marina OX=82927 GN=CYP716A259 PE=2 SV=1	[more]
A0A1B0VRJ3	8.1e-134	75.00	Cytochrome P450 CYP716A67 OS=Plectranthus barbatus OX=41228 PE=2 SV=1	[more]
A0A1S4CJC5	1.5e-132	74.58	beta-amyrin 28-oxidase-like OS=Nicotiana tabacum OX=4097 GN=LOC107819508 PE=3 SV...	[more]

BLAST of Spg021918 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G36110.1	1.2e-105	58.14	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]
AT5G36130.1	2.3e-56	67.14	Cytochrome P450 superfamily protein	[more]
AT2G42850.1	8.8e-56	39.05	cytochrome P450, family 718	[more]
AT5G45340.1	7.2e-50	37.12	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT2G29090.1	9.4e-50	35.31	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 103..120 score: 21.03 coord: 209..233 score: 28.81 coord: 251..274 score: 30.39 coord: 241..251 score: 49.89
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 114..131 score: 26.8 coord: 170..181 score: 37.96 coord: 251..262 score: 38.54 coord: 242..251 score: 58.31
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 42..285 e-value: 1.2E-56 score: 192.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..301 e-value: 3.8E-85 score: 288.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 2..301
None	No IPR available	PANTHER	PTHR24286:SF283	BETA-AMYRIN 28-OXIDASE-LIKE	coord: 2..300
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 2..300
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 244..253

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg021918.1	Spg021918.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen