Spg021901 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg021901
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionUbiquitin carboxyl-terminal hydrolase-related protein
Locationscaffold2: 5136152 .. 5145643 (+)
RNA-Seq ExpressionSpg021901
SyntenySpg021901
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTAATTTTTTTTTACTCTCTCGCTCTCTCTCTCTTCTATCTTTCTCTCTCTCTCTCATCAAATCTCAGCACCCCCCCTCCCCCTTCCGAGGGTAGGTGTCTGTGTGTCACACACAGAGAGACCACTCTACTTCCTTCCCCGCCTAATCCCTTCTCCCTCTTTGATTTCTTTGATGATCCATCTTCTTCTCCGGCGACGGCAATCTCCTCCCATTTTCCGCCACCGGAATCTGTACGTCGACGACTCTGTACCCTTCCTACATCTTCTTCTACCTTCAATCCGCGAGTAAGTTTGTTTTACTCCCTTTTCTTATTGTTGTTGTATCGGTAGACGCATATATAGAATTTTCTATATGCATGTCAATTGGATTGCTATAGAGTTTATAGATGGGGCGTAAGAAGAAAGTCATTGATCCGCGTTCTAAACAATCTCCGGCGGCTCGAGGAGTCGCCGTTGATGTTCCAAGTTTAGCGAACGCCGTCGACCACAGTTCGATACCTGATAAGGCAAGTAATCACAACCAAAGTAGGATCGAGCCGGTGTTTGTCAATGAATCGGATGGCTTGTCTTATTCCTCAATTAAACTCGAATGCGAGCGGGCTCTTACCGCGCTTCGGCGTGGGAACCACACTAAGGCACTTAGGCTTATGAAGGAGTTGAGCTCCAGAAATGAGAACTCTGTTCACTCTGCATTGATTCATCGGGTTCAGGGTACTATTTGTGTGAAAGTAGCATCCATTATTGATGACCTGAATACAAAGCAGCGGCATTTGAAGAATGCGATTGAGTCTGCTCGTAAAGCAGTTGAGCTCTCACCAAACTCGATCGAGTTTTCCCATTTCTATGCAAATTTGCTGTACGAGGTTGCAAATGATGCGAAAGAGTATGAGGAGGTGGTGCAAGAGTGTGAGCGGGCCCTTGGCATTGAAAACCCAATCGATCCCGCTAAGGAAAGCTTGCAGGATGAAAAGAATCAGAAGATACCTACTGCAGAAGCACGGATTACACACGTTCAGACTGAATTGAGGCAGTTAATTCAAAAGTCAAATATATATTCCCTATCATCTTGGATGAAGAACTTGGGTAACGGGGAGGAGAAGTTTAGGTTGATTCCGATTAGGAGAGTGACAGAGGACCCAATGGAGGTGAGGTTGGTTCAGGCTAGAAGGGCAAATGAAATTAAGAAGGCAACCAAAACGCCTGAGGAACGAAGGAAGGAGATCGAAGTCCGTGTTGCTGCTGCAAGGCTTTTGCAACAGAAGTCTGAGACACCTCAAATGCAAAACGAAGGAGGTAAAGCTGATCGGGCCCTGGATTCATCTTCTGGGTCTGGTACAAGAGTTGGTGAGCGGCGGAAACATGGAAATTTACGGAAACATGGATCGTCAGCTGAAAGGAGGGATTGGGTTCATTCTTTTTGGAATTCCATGAGTTTGGAATCAAAGAAAGATTTGCTTAAGATCAAGACAAGTGATCTTAAGACTCATTTTAGTTCTTCGAAGGATGCTTCGGCAAATGAAGTTATTTCAGAAGCTTTGTCCTTTTATGATGCCAATAAAACATGGAAATTTTGGGCTTGCTGCAGATGTGATGAAAAATTTGTGGATTCAGAATCCCATATGCACCATGTGGTTCAGGAGCACATGGGTAACCTTTTGCCGAAAATGCAATCTATCTTGCCACAGAATGTTGACAATGAATGGACCGAAATGCTTCTTAATTGCTCTTGGAAAGCGTTGGATGTTTCAGCTGCAGCTAAAATGCTCACAGATCAAAGAAAGTTCAAAGATTCCGAGTTGGATGAAGATATCTGCTCACAGCATCATTCAGAATCTGATGACTGTTTCAAAGATGCATGGGATTCTTCTCCAGTGAAGCATAGATATGGGAATAGTTTAAATGAATGCAATCTTTATGAAAAAGTTAGCAACAACGGATTTAAGACCTGTAATGAAAACCAGGGTTCTTTGGTATCATATTCCTTATCTAATAGCTTTCCAGTATCTGATGACTCTGAGCGTACAAAGCTTCTTGAGAAAATTCATGCAGTATTTGAGTTACTTATTCGACATAAATATCTTGCTGCAAGCCATCTAAACAAGGTTATACAATTTACAATGGATGAGTTACAGGGTCTTGCTTCTGGATCTCATCTTCTAAACCAGGGTCTGGACCAAACACCACTATGCATTTGCTTTCTAGGTGCTTCTCAGCTTAGGAAAATCCTCAAGTTCTTGCAAGAACTATGTCAGTCTTGTGGTATTGGTAGATACTCTGATAAAAGTACTGGTCAAATTGAAGATTCAAAGAGTGATAAACAACCTGTTGAAGTCAAAGAGAAAGTTGTTCTCAACAGAGATGCGTCGCTTCTCATCCTCAATGAGTGTTTTCTGTCATCTGATATCCCTCATCCCAATGGTCAGGTTGCAGCCACAGATCATATGCCTGCAACTGCAAAGGATGTTATTGCAAATGGGGTTCTGTCTGAAGTCGATCCTTTTCTATCCTGGATATATGGAGGTCCCTCTAGTGGGGAGCAGTTAGCATCATGGGCACAATCAAAGGAAGAAAAGATACAGCGACGAATGGAAATTTTTCAAATGCTTGAGAAGGAATTTTACCATCTGCAGAGCCTTTGTGAGAGAAAATGTGAGCATTTGAACTACGAGGAAGCATTGCAGTCAGTAGAGGATCTCTGCCTTGAAGAGGGCAAGAAGAGGGAAACTGTGACTGAATTTATCCCCAAGAGTTATGAGTCAGTCCTGAGGAGACGAAGAGATGAGCTTATTGAGGCTGAAAATGACGTGATGTTTATCGGCAGTAGGTTTGAGTTAGATGCTGTAACTAATGTTTTGAAAGAAGCTGAAGCTCTGAATGTTAATCAATTTGGATATGAGGAAAATTTTGCCAGCGTACCTCCTCCATTATGTGATCTGGAATCTGGTGAAGATGAAGATTGGAGGGCTAAGGATTATCTGCATCAAGTTGATACTTGTATTGAAGTTGCAATACAGAGGCAGAAAGAACAGTTATCTATCGAGGTTTATTTATTAATTACGGATGCTTTACATATATTTTGAGATATTTGCTATTTGCTTATTTTATTTGATTGTGCAGATTAGCAAAATTGATGCACGGATCATGCGAAATGTTACTGGAATGCAACAGTTGGAACTTAAGCTTGAACCTGTTTCTGCACATGATTATCAATCAATATTGTTGCCTCTTGTGAACTCATACTTGAGGGTCTGTATCAAATAGGTTAATTTCTCCTAATATTCCTTGAGCATTTTGGGACTAAAATGACAGATTCAAATATCAAACTATAACTTTCAGGCACATTTAGAAGATTTGGCTGAAAAAGATGCCACAAAGAAGTCTGATGCTGCACGAGAAGCATTTTTAGCTGAATTGGCACTGGATTCAAAAAAGGGTATCAGAGGAGGAAATGATAATTCAAGACATGCACATGAGAAGCCGAAGGAAAAGAAAAGGAGCAAGGAGTTTAGGAAAGTCAAGGACTCTAAGGTTGTCTGTCATTTTACTATTAGAAGTTTAGGTTCATGGTTTTTTTGTTCTTGTAACTTAGTTGTTCTTGGTGCCTATTGATATTTTAATACTTCTACTTCTGCAGGTGGTCAGTGGTCCTGAGCAGGATGTGCTTCATGATGAGGCTGTGGATGGAGAGTAAGTGTTACTGATGACTTTACCACTCTCAAACCTCTTCATCACTTGTAGGCATTTGTTTTATTATAATATCACGAAGGTCTGTAGTCTTCTATCTAATTTTCTGTTCTTGGTGGGTGTTTGTCAATTACTTGTTGCAGTCCCTTTCAAGTTTCATCTGATGGTGATGCTGCAGAGGTGGACATTGCTGTTTTTGAGAATGGTGATGCTTTAAGACTACAGGAGGAAGAGTTTAGACGCAAAATTGAGCTGGAAGCAGATGAAAGAAAGCTAGAAGAGACTTTGGAGTATCAAAGACGAATTGAGAATGAGGCTAAACAAAAGCATCTGGCTGAACTACAAAAGAAATCTGCCCAGACAAATCTAGAGAAGAACTTGGCTGTGGCGGTACATGATAATCTCATTGGACTTGTCCCCAATGTTGAGGGTGCTGATGAGTATCTTAAGCCCTCTGTGGTGGTAAATGTTCTGTACTTTGTCATTCTTTCCCCTTTTTGTTATTTAAATGAAATTATGGAACTCCCTAGTTCTACATGTATCCAACATACAAATTTGATAAAATACTTAAGAGGATCTTATAAATTGCTATTTTAGGATCAATTGGCAGAAAATGATTTGGTTGCCGACTTGTCTAGGAATGGTCCTGCAATGCAAGGCGCTTTTTCAGGTGATCAAAAGATTAGTGGTGTGCAATGATGGAAAGTTACTTGGCAGAAAGATGTTGTGGTGTTTAATGTTGGATTCGTCATTCTCTTTTTCTTTAGCAGGTATATCCAATGTAGGAGTTCTGGAGAATAGTCTGCCATCTTCTGACAGGAGGAAGGGGAGGAAAGGTAGACGGCACAAGGTTTCAATCAAACCAGTAGATGGAAATCAGCCTTCACCATCTGATAAGGATAATGTAACCTTTGACAACCAATTGAATGAGCAAGTTAGATATCATGACAATCTCCTAGTAGATAGTGGTGAGTGCATATATTTCATTTTGAAATCCACTATTTCTTTTATCGTCATTAATAAAGGGGATTGTGAATGATGGACTTGTTTTGGGTTTTATTCGTTTTAAATGATTCATACATAATGAAGCTGGGTGTGCAAGTAGAAACCGATAATAATCAAAATCCATTTCCATTATTGATGAATTTTCATCGACCAAATGCCACAAATTCTAGTGTTCTGTTTTGTCATGGATTTTTTTCCACTTAAGGTCATTTGCAACGAAAGATATAGGATTTTAACTGAAAGGAATTTTCTTTTCTCCCTAAAACAAATTTCATTGATAAAATTCTTTTATTCAGGTCTATAGTCAACTTCTCCTTGCATGATATATTTGTTGTGAAAGTTGTATTCTCTCGCTCACTCGTTGAAGATATTGAAAGTATTTGTAAATATTAAATTTTGCTATTCATACTTCGATCATTAGCATGCCTGTGTAAATCTGCAATGTGAGGTCACTGTTCATCTTCTTTTTGTTTAGACTAAACTAATATTGTCTTATATTCTGGTTCAATCAGTTAATCCACTTTCCGAAGATAATAGTACAAAGACTTTGAGACGACATGTAGAGGATGATGAGGAAAGATTTCAAGCTGATCTTAAGAAGGCTGTACGCCAGAGCCTTGGTTAGGTTCCTCGTGTCTTTTCTCTTTTCGCTTTTAGTCATTGCACTCTTATGATTTTAATTTGGTTAATTTGTATTTGGCAGGAAAAATGGTTAAAAAGATCACAAATTATTCTATGGGATACCTTTTTACTACAATTTTTGCCCGTAGATTTGCTCTTATGTTTTTGTATGTAATAAAGCTCCATTGTCTCTCTATTATGCTTGATTAGATGGAGCCATGGTCAATTATTTTTTCTTTAACTTAGCAAATGGTTTAGTCTTTGTCCCGATGTCAAAATTTTTGGCCTGTTTTCCTTCTCTCTAGTTCTTTGGATTCAGGATATGATTGTCTCAAAATGCTCACACTATAAACTTTTTAATAACTAAAGAAAGCCATATAAGATATTGTAACTTTTTAGATGTTAATCTTTAATTTGCTTCAATCTGGATGAATGATTAGATTTCTAAAGGTTTCCTGCATTTATGGGCGCTTCTTTCTATTTCCTTTTCTGTATAGTAACTGCATATATATATATATATTTAAATAGTAACTGCATATTTTCTAACCGTAATATTAATGTTTTCTCAGATGCATTTCAAGAACAGCAGAAATTACCCTTGATTACAAATTCCAGGACACCGTTGATCAGTTATGGTGAAGTAGATAGTAATGGTCTTCCATCAAATGAACTCAATGTTGAAAATGTGGGAGCAGATATATTTGGTACAGGACTTAAGAATGAAGTTGGTGAATATAATTGCTTTCTGAATGTGATTATTCAAGTGAGTGCTTGACAGAAGAAGATTATATATCACTTCACTTTGCTAGTTTGTTCCTCTTTCTGCATATGCAGTCTAAAATGTTGTCTTGTTTTACATGAGCTCAGTCCTTATGGCATTTAAGACGGTTTCGAGAAGAGTTCCTGAGAAGATCTATGACTGAGCATGTTCATGTTGGGGATCCCTGTGTTGTTTGTGCATTATATGAAATATTCGCTGCCTTGAGCATGGCATCTGCAGATGCTAGAAGAGAAGCAGTCGCCCCCACTTCTTTGAGAATAGCTCTGAGCAATCTTTGTCCTGATAGTAATTTCTTCCAAGAGGTAGTTTTGCATACCCCTGGTTTTTGACCCCTACAACTTAAAAGAGAAAGAAAGAAAGCGATTCTACTCTACTCAATTTATTGTAGTGATTATGAGAAAGTCTATAACTAATTTTTGACTGTTGTTTAGGGCCAGATGAATGACGCCTCTGAAGTGCTGGCAGTCATATTTGATTGCCTACACCAGTCATTGACTCCTAGTTCCAGCGTTTCTGATACAGAATCAGTTGAAAGCAATTGCATGGGGTCTTGGGACTGTGCAAGTGCAACTTGTTTGGTACATTCCATCTTCGGAATGGACATTTTTGAACGAATGAATTGCTATAGTTGTGGCCTGGAATCTAGACATCTGAAGTACACTTCCTTCTTTCACAATATAAATGCAAGTGCCCTTCGAACAATGAAGGTAATAGAATCCACCTTTCCTTGTCGTTGAAGATCATTCTTTTAAGGACAAGACAATATTCTTATATTGAACTCTGTTTCTTTAATTCAATTTTCTTCATTATTCAGGTTATGTGTACAGAAAGTTCCTTTGATGAACTTCTGAATGTTGTTGAAATGAATCATCAGCTAGCTTGTGATTTGGATGTTGGTGGTTGTGGAAAGCTCAATTACATTCATCACTTTCTCTCTGCACCGCCACACGTGTTCACAACTGGTGATTCAAATAATTTTCGTCCAACTCCTACTCACCCTTTTCCCTCCCTCCCATTACCCCTGATCTATTATTCATGATGTTTTACTTGTCTGTAATTTTGGTATTGCAGTCTTGGGCTGGCAGAACACTTGTGAGAGTGCTGATGACATCACAGCTACATTAGCTGCTCTAAGTACTGAGATAGATATCAGCGTTCTTTATCGGGGTCTAGATCCAAAGCACACCCATAATTTGGTATCAGTGGTATGTACTCTTTGTTAATTTGTATGTGTATGTACTCGTAGGGTGGAGCTTATTTTTATATGTCAAAATGTTTTACTCGGAGACTGTTTTCTTGAATGGTTTAGAGACTTTTCATGGGTATGAAGTTTTAATTCCTTGCTCATCATGACCGTGGATTTTGAGAATACCTAATACTCATCGATATTCAAAAGGAATATTGTATTGAAAAAATTTGCAAATATCCCAAATCTAAGTCTATCATTGATAGACTTCAATCACAATGCATGTAGTCTATTAGTGATAGAATAGTCTACCAGCCATAATTGTAATTATGATTAAAGTCTATCGTTGATAGACTTTAATTTTGTTATATTTCCAAATAGTTTTAGCAAATCTATAAGTGATACAGTCAGAATGCATGTAGTCTATCAATGATAGACTACTATTACTGATAGAATAATCGATTAGCAATGATTACACTTGTGATTAAAGTCTATCGGTAATAGACTTCTATACTGATAAACTTCAATTTTGCTATATTTGCAATTATTTTAGTCTATTTTGCTATTTGTGCAGACAACCCTATTCAAAACATGTCAACCACGGGTTTTGAGTATTCTTAGGCATTCATAGACTGATAGACATTCAGAACACCTGGGCTTCTTGCATTTCTTTGTACATGAGGATGTTCATGGCTATTTTTTGTTACCGAATTTTCCCTCGACTCTTTACTACTTTCCTGTTAAGACGGGGCATGAATTCTGTTCAAATTTGATTCATTGTAATTTTATTATTATTCTTTCTTAATTTTTTAACGAGTAATATTATTCTTAACATGTTTATCTACATTTGGTTTTTCGTTAAGCATATTCTGCACAGAAAACAATAAAATCTAGCATCTCCAGATATACTAAATACAGCAAACACAGTTATTTAACATTTTAAGACAACTAAAAGTTTATTATTATGCATTGACATGATCCTCTCTAGCAACTCTTAATACTTGAATCAAATGACCTCTTGGGGTTTTAACATAAGAAGGTTAGGTTTGGATGTTAGTTTTGATGCAAGGCCTTAAATTAATCATCTATAAGCATCCATTGTCTTGCTAAGGAATCAACACCGACCATTTTCCTTTGACATAGAGGGGTAGTACTGGTGCTTCTGGAGTATGTCATCTTTAAATATGTTTCTTCATTACGTATTTGCCAAAATATGAAGTCTTCAGTTTACTGAAAACTAATTAATGCACCCAACTTTGTAGGTTTGCTATTATGGTCAACACTATCACTGTTTTGCCTACAGTCACGACAAAAAATGCTGGATAATGTATGATGATAGAACTGTTAAGGTAACTTCTTACTTTCCCCTGCCTCTTTTTCCTTTTTTGGGGCATGTATGGCATGTGTGTGTGTTTCAGTTTCTCTAATGTGGCATAAACTGCGCTCATGCCTAGGTAATTGGTGGCTGGCCTGACGTTCTTACCATGTGTGAAAAAGGACACTTGCAACCTCAGGTTCTTTTCTTTGAAGCTGTAAATTAGCGATATTTTGGAACTGTCTCATTAGCCATACTGGTGGAACGTGGTTTACATTTGATACCTGCCTTGGCAAGGAGATGTTTATAGGGATGACTTGCCATGTACATCCACGGAATTCAGACAGTAACGTGTAATATTGGTTTCAAAAGAAAAATTTTGATCGGGTCATAACATAAATCTCTGAGTTTTCTGATACAATCAACAAATTTTTGGAGAAGATCTGGTCCTCGAGGAGAATATTGTAAGTATGATAGATTTTCTTCTAAATTGAGGGAGCCAGATGATTGGAGAGCCAAAAGTGAGATGAAGCACCCAGGTTTCTGCCTCTGGAATTTCTTCTTGGCTTTGATCCTTTAAATAAGGTGTTAGAGAAAGTAAGAAATGGTGTTAAATGGGTGGTTTTTTGTGATATCTTACTTAGGAGGTTTTATAGCTGTATCATAAGAGTAATGATTTCTGGTTGTTGAGAAGACCAACATAGCGAACAGTTGAGACAGAGTCTAGTTAGAGACAATGCTAGATGCTTTTCATCTTTCTCATCTACTTTTCTGCAGTTTTAGGTGTTGTATAGTTCCAATTAAAGTCACTCAAGCCTCTGGCTATGGTCACTCTAAAGTGCAATTATTTTGTCATCCAGATTTTGGTGATCAATATGAAACAATCTTCAAGTCTCCT

mRNA sequence

ATGGGGCGTAAGAAGAAAGTCATTGATCCGCGTTCTAAACAATCTCCGGCGGCTCGAGGAGTCGCCGTTGATGTTCCAAGTTTAGCGAACGCCGTCGACCACAGTTCGATACCTGATAAGGCAAGTAATCACAACCAAAGTAGGATCGAGCCGGTGTTTGTCAATGAATCGGATGGCTTGTCTTATTCCTCAATTAAACTCGAATGCGAGCGGGCTCTTACCGCGCTTCGGCGTGGGAACCACACTAAGGCACTTAGGCTTATGAAGGAGTTGAGCTCCAGAAATGAGAACTCTGTTCACTCTGCATTGATTCATCGGGTTCAGGGTACTATTTGTGTGAAAGTAGCATCCATTATTGATGACCTGAATACAAAGCAGCGGCATTTGAAGAATGCGATTGAGTCTGCTCGTAAAGCAGTTGAGCTCTCACCAAACTCGATCGAGTTTTCCCATTTCTATGCAAATTTGCTGTACGAGGTTGCAAATGATGCGAAAGAGTATGAGGAGGTGGTGCAAGAGTGTGAGCGGGCCCTTGGCATTGAAAACCCAATCGATCCCGCTAAGGAAAGCTTGCAGGATGAAAAGAATCAGAAGATACCTACTGCAGAAGCACGGATTACACACGTTCAGACTGAATTGAGGCAGTTAATTCAAAAGTCAAATATATATTCCCTATCATCTTGGATGAAGAACTTGGGTAACGGGGAGGAGAAGTTTAGGTTGATTCCGATTAGGAGAGTGACAGAGGACCCAATGGAGGTGAGGTTGGTTCAGGCTAGAAGGGCAAATGAAATTAAGAAGGCAACCAAAACGCCTGAGGAACGAAGGAAGGAGATCGAAGTCCGTGTTGCTGCTGCAAGGCTTTTGCAACAGAAGTCTGAGACACCTCAAATGCAAAACGAAGGAGGTAAAGCTGATCGGGCCCTGGATTCATCTTCTGGGTCTGGTACAAGAGTTGGTGAGCGGCGGAAACATGGAAATTTACGGAAACATGGATCGTCAGCTGAAAGGAGGGATTGGGTTCATTCTTTTTGGAATTCCATGAGTTTGGAATCAAAGAAAGATTTGCTTAAGATCAAGACAAGTGATCTTAAGACTCATTTTAGTTCTTCGAAGGATGCTTCGGCAAATGAAGTTATTTCAGAAGCTTTGTCCTTTTATGATGCCAATAAAACATGGAAATTTTGGGCTTGCTGCAGATGTGATGAAAAATTTGTGGATTCAGAATCCCATATGCACCATGTGGTTCAGGAGCACATGGGTAACCTTTTGCCGAAAATGCAATCTATCTTGCCACAGAATGTTGACAATGAATGGACCGAAATGCTTCTTAATTGCTCTTGGAAAGCGTTGGATGTTTCAGCTGCAGCTAAAATGCTCACAGATCAAAGAAAGTTCAAAGATTCCGAGTTGGATGAAGATATCTGCTCACAGCATCATTCAGAATCTGATGACTGTTTCAAAGATGCATGGGATTCTTCTCCAGTGAAGCATAGATATGGGAATAGTTTAAATGAATGCAATCTTTATGAAAAAGTTAGCAACAACGGATTTAAGACCTGTAATGAAAACCAGGGTTCTTTGGTATCATATTCCTTATCTAATAGCTTTCCAGTATCTGATGACTCTGAGCGTACAAAGCTTCTTGAGAAAATTCATGCAGTATTTGAGTTACTTATTCGACATAAATATCTTGCTGCAAGCCATCTAAACAAGGTTATACAATTTACAATGGATGAGTTACAGGGTCTTGCTTCTGGATCTCATCTTCTAAACCAGGGTCTGGACCAAACACCACTATGCATTTGCTTTCTAGGTGCTTCTCAGCTTAGGAAAATCCTCAAGTTCTTGCAAGAACTATGTCAGTCTTGTGGTATTGGTAGATACTCTGATAAAAGTACTGGTCAAATTGAAGATTCAAAGAGTGATAAACAACCTGTTGAAGTCAAAGAGAAAGTTGTTCTCAACAGAGATGCGTCGCTTCTCATCCTCAATGAGTGTTTTCTGTCATCTGATATCCCTCATCCCAATGGTCAGGTTGCAGCCACAGATCATATGCCTGCAACTGCAAAGGATGTTATTGCAAATGGGGTTCTGTCTGAAGTCGATCCTTTTCTATCCTGGATATATGGAGGTCCCTCTAGTGGGGAGCAGTTAGCATCATGGGCACAATCAAAGGAAGAAAAGATACAGCGACGAATGGAAATTTTTCAAATGCTTGAGAAGGAATTTTACCATCTGCAGAGCCTTTGTGAGAGAAAATGTGAGCATTTGAACTACGAGGAAGCATTGCAGTCAGTAGAGGATCTCTGCCTTGAAGAGGGCAAGAAGAGGGAAACTGTGACTGAATTTATCCCCAAGAGTTATGAGTCAGTCCTGAGGAGACGAAGAGATGAGCTTATTGAGGCTGAAAATGACGTGATGTTTATCGGCAGTAGGTTTGAGTTAGATGCTGTAACTAATGTTTTGAAAGAAGCTGAAGCTCTGAATGTTAATCAATTTGGATATGAGGAAAATTTTGCCAGCGTACCTCCTCCATTATGTGATCTGGAATCTGGTGAAGATGAAGATTGGAGGGCTAAGGATTATCTGCATCAAGTTGATACTTGTATTGAAGTTGCAATACAGAGGCAGAAAGAACAGTTATCTATCGAGATTAGCAAAATTGATGCACGGATCATGCGAAATGTTACTGGAATGCAACAGTTGGAACTTAAGCTTGAACCTGTTTCTGCACATGATTATCAATCAATATTGTTGCCTCTTGTGAACTCATACTTGAGGGCACATTTAGAAGATTTGGCTGAAAAAGATGCCACAAAGAAGTCTGATGCTGCACGAGAAGCATTTTTAGCTGAATTGGCACTGGATTCAAAAAAGGGTATCAGAGGAGGAAATGATAATTCAAGACATGCACATGAGAAGCCGAAGGAAAAGAAAAGGAGCAAGGAGTTTAGGAAAGTCAAGGACTCTAAGGTGGTCAGTGGTCCTGAGCAGGATGTGCTTCATGATGAGGCTGTGGATGGAGATCCCTTTCAAGTTTCATCTGATGGTGATGCTGCAGAGGTGGACATTGCTGTTTTTGAGAATGGTGATGCTTTAAGACTACAGGAGGAAGAGTTTAGACGCAAAATTGAGCTGGAAGCAGATGAAAGAAAGCTAGAAGAGACTTTGGAGTATCAAAGACGAATTGAGAATGAGGCTAAACAAAAGCATCTGGCTGAACTACAAAAGAAATCTGCCCAGACAAATCTAGAGAAGAACTTGGCTGTGGCGGTACATGATAATCTCATTGGACTTGTCCCCAATGTTGAGGGTGCTGATGAGTATCTTAAGCCCTCTGTGGTGGATCAATTGGCAGAAAATGATTTGGTTGCCGACTTGTCTAGGAATGGTCCTGCAATGCAAGGCGCTTTTTCAGGTATATCCAATGTAGGAGTTCTGGAGAATAGTCTGCCATCTTCTGACAGGAGGAAGGGGAGGAAAGGTAGACGGCACAAGGTTTCAATCAAACCAGTAGATGGAAATCAGCCTTCACCATCTGATAAGGATAATGTAACCTTTGACAACCAATTGAATGAGCAAGTTAGATATCATGACAATCTCCTAGTAGATAGTGTTAATCCACTTTCCGAAGATAATAGTACAAAGACTTTGAGACGACATGTAGAGGATGATGAGGAAAGATTTCAAGCTGATCTTAAGAAGGCTGTACGCCAGAGCCTTGATGCATTTCAAGAACAGCAGAAATTACCCTTGATTACAAATTCCAGGACACCGTTGATCAGTTATGGTGAAGTAGATAGTAATGGTCTTCCATCAAATGAACTCAATGTTGAAAATGTGGGAGCAGATATATTTGGTACAGGACTTAAGAATGAAGTTGGTGAATATAATTGCTTTCTGAATGTGATTATTCAATCCTTATGGCATTTAAGACGGTTTCGAGAAGAGTTCCTGAGAAGATCTATGACTGAGCATGTTCATGTTGGGGATCCCTGTGTTGTTTGTGCATTATATGAAATATTCGCTGCCTTGAGCATGGCATCTGCAGATGCTAGAAGAGAAGCAGTCGCCCCCACTTCTTTGAGAATAGCTCTGAGCAATCTTTGTCCTGATAGTAATTTCTTCCAAGAGGGCCAGATGAATGACGCCTCTGAAGTGCTGGCAGTCATATTTGATTGCCTACACCAGTCATTGACTCCTAGTTCCAGCGTTTCTGATACAGAATCAGTTGAAAGCAATTGCATGGGGTCTTGGGACTGTGCAAGTGCAACTTGTTTGGTACATTCCATCTTCGGAATGGACATTTTTGAACGAATGAATTGCTATAGTTGTGGCCTGGAATCTAGACATCTGAAGTACACTTCCTTCTTTCACAATATAAATGCAAGTGCCCTTCGAACAATGAAGGTTATGTGTACAGAAAGTTCCTTTGATGAACTTCTGAATGTTGTTGAAATGAATCATCAGCTAGCTTGTGATTTGGATGTTGGTGGTTGTGGAAAGCTCAATTACATTCATCACTTTCTCTCTGCACCGCCACACGTGTTCACAACTGTCTTGGGCTGGCAGAACACTTGTGAGAGTGCTGATGACATCACAGCTACATTAGCTGCTCTAAGTACTGAGATAGATATCAGCGTTCTTTATCGGGGTCTAGATCCAAAGCACACCCATAATTTGGTATCAGTGGTTTGCTATTATGGTCAACACTATCACTGTTTTGCCTACAGTCACGACAAAAAATGCTGGATAATGTATGATGATAGAACTGTTAAGGTAATTGGTGGCTGGCCTGACGTTCTTACCATGTGTGAAAAAGGACACTTGCAACCTCAGGTTCTTTTCTTTGAAGCTGTAAATTAG

Coding sequence (CDS)

ATGGGGCGTAAGAAGAAAGTCATTGATCCGCGTTCTAAACAATCTCCGGCGGCTCGAGGAGTCGCCGTTGATGTTCCAAGTTTAGCGAACGCCGTCGACCACAGTTCGATACCTGATAAGGCAAGTAATCACAACCAAAGTAGGATCGAGCCGGTGTTTGTCAATGAATCGGATGGCTTGTCTTATTCCTCAATTAAACTCGAATGCGAGCGGGCTCTTACCGCGCTTCGGCGTGGGAACCACACTAAGGCACTTAGGCTTATGAAGGAGTTGAGCTCCAGAAATGAGAACTCTGTTCACTCTGCATTGATTCATCGGGTTCAGGGTACTATTTGTGTGAAAGTAGCATCCATTATTGATGACCTGAATACAAAGCAGCGGCATTTGAAGAATGCGATTGAGTCTGCTCGTAAAGCAGTTGAGCTCTCACCAAACTCGATCGAGTTTTCCCATTTCTATGCAAATTTGCTGTACGAGGTTGCAAATGATGCGAAAGAGTATGAGGAGGTGGTGCAAGAGTGTGAGCGGGCCCTTGGCATTGAAAACCCAATCGATCCCGCTAAGGAAAGCTTGCAGGATGAAAAGAATCAGAAGATACCTACTGCAGAAGCACGGATTACACACGTTCAGACTGAATTGAGGCAGTTAATTCAAAAGTCAAATATATATTCCCTATCATCTTGGATGAAGAACTTGGGTAACGGGGAGGAGAAGTTTAGGTTGATTCCGATTAGGAGAGTGACAGAGGACCCAATGGAGGTGAGGTTGGTTCAGGCTAGAAGGGCAAATGAAATTAAGAAGGCAACCAAAACGCCTGAGGAACGAAGGAAGGAGATCGAAGTCCGTGTTGCTGCTGCAAGGCTTTTGCAACAGAAGTCTGAGACACCTCAAATGCAAAACGAAGGAGGTAAAGCTGATCGGGCCCTGGATTCATCTTCTGGGTCTGGTACAAGAGTTGGTGAGCGGCGGAAACATGGAAATTTACGGAAACATGGATCGTCAGCTGAAAGGAGGGATTGGGTTCATTCTTTTTGGAATTCCATGAGTTTGGAATCAAAGAAAGATTTGCTTAAGATCAAGACAAGTGATCTTAAGACTCATTTTAGTTCTTCGAAGGATGCTTCGGCAAATGAAGTTATTTCAGAAGCTTTGTCCTTTTATGATGCCAATAAAACATGGAAATTTTGGGCTTGCTGCAGATGTGATGAAAAATTTGTGGATTCAGAATCCCATATGCACCATGTGGTTCAGGAGCACATGGGTAACCTTTTGCCGAAAATGCAATCTATCTTGCCACAGAATGTTGACAATGAATGGACCGAAATGCTTCTTAATTGCTCTTGGAAAGCGTTGGATGTTTCAGCTGCAGCTAAAATGCTCACAGATCAAAGAAAGTTCAAAGATTCCGAGTTGGATGAAGATATCTGCTCACAGCATCATTCAGAATCTGATGACTGTTTCAAAGATGCATGGGATTCTTCTCCAGTGAAGCATAGATATGGGAATAGTTTAAATGAATGCAATCTTTATGAAAAAGTTAGCAACAACGGATTTAAGACCTGTAATGAAAACCAGGGTTCTTTGGTATCATATTCCTTATCTAATAGCTTTCCAGTATCTGATGACTCTGAGCGTACAAAGCTTCTTGAGAAAATTCATGCAGTATTTGAGTTACTTATTCGACATAAATATCTTGCTGCAAGCCATCTAAACAAGGTTATACAATTTACAATGGATGAGTTACAGGGTCTTGCTTCTGGATCTCATCTTCTAAACCAGGGTCTGGACCAAACACCACTATGCATTTGCTTTCTAGGTGCTTCTCAGCTTAGGAAAATCCTCAAGTTCTTGCAAGAACTATGTCAGTCTTGTGGTATTGGTAGATACTCTGATAAAAGTACTGGTCAAATTGAAGATTCAAAGAGTGATAAACAACCTGTTGAAGTCAAAGAGAAAGTTGTTCTCAACAGAGATGCGTCGCTTCTCATCCTCAATGAGTGTTTTCTGTCATCTGATATCCCTCATCCCAATGGTCAGGTTGCAGCCACAGATCATATGCCTGCAACTGCAAAGGATGTTATTGCAAATGGGGTTCTGTCTGAAGTCGATCCTTTTCTATCCTGGATATATGGAGGTCCCTCTAGTGGGGAGCAGTTAGCATCATGGGCACAATCAAAGGAAGAAAAGATACAGCGACGAATGGAAATTTTTCAAATGCTTGAGAAGGAATTTTACCATCTGCAGAGCCTTTGTGAGAGAAAATGTGAGCATTTGAACTACGAGGAAGCATTGCAGTCAGTAGAGGATCTCTGCCTTGAAGAGGGCAAGAAGAGGGAAACTGTGACTGAATTTATCCCCAAGAGTTATGAGTCAGTCCTGAGGAGACGAAGAGATGAGCTTATTGAGGCTGAAAATGACGTGATGTTTATCGGCAGTAGGTTTGAGTTAGATGCTGTAACTAATGTTTTGAAAGAAGCTGAAGCTCTGAATGTTAATCAATTTGGATATGAGGAAAATTTTGCCAGCGTACCTCCTCCATTATGTGATCTGGAATCTGGTGAAGATGAAGATTGGAGGGCTAAGGATTATCTGCATCAAGTTGATACTTGTATTGAAGTTGCAATACAGAGGCAGAAAGAACAGTTATCTATCGAGATTAGCAAAATTGATGCACGGATCATGCGAAATGTTACTGGAATGCAACAGTTGGAACTTAAGCTTGAACCTGTTTCTGCACATGATTATCAATCAATATTGTTGCCTCTTGTGAACTCATACTTGAGGGCACATTTAGAAGATTTGGCTGAAAAAGATGCCACAAAGAAGTCTGATGCTGCACGAGAAGCATTTTTAGCTGAATTGGCACTGGATTCAAAAAAGGGTATCAGAGGAGGAAATGATAATTCAAGACATGCACATGAGAAGCCGAAGGAAAAGAAAAGGAGCAAGGAGTTTAGGAAAGTCAAGGACTCTAAGGTGGTCAGTGGTCCTGAGCAGGATGTGCTTCATGATGAGGCTGTGGATGGAGATCCCTTTCAAGTTTCATCTGATGGTGATGCTGCAGAGGTGGACATTGCTGTTTTTGAGAATGGTGATGCTTTAAGACTACAGGAGGAAGAGTTTAGACGCAAAATTGAGCTGGAAGCAGATGAAAGAAAGCTAGAAGAGACTTTGGAGTATCAAAGACGAATTGAGAATGAGGCTAAACAAAAGCATCTGGCTGAACTACAAAAGAAATCTGCCCAGACAAATCTAGAGAAGAACTTGGCTGTGGCGGTACATGATAATCTCATTGGACTTGTCCCCAATGTTGAGGGTGCTGATGAGTATCTTAAGCCCTCTGTGGTGGATCAATTGGCAGAAAATGATTTGGTTGCCGACTTGTCTAGGAATGGTCCTGCAATGCAAGGCGCTTTTTCAGGTATATCCAATGTAGGAGTTCTGGAGAATAGTCTGCCATCTTCTGACAGGAGGAAGGGGAGGAAAGGTAGACGGCACAAGGTTTCAATCAAACCAGTAGATGGAAATCAGCCTTCACCATCTGATAAGGATAATGTAACCTTTGACAACCAATTGAATGAGCAAGTTAGATATCATGACAATCTCCTAGTAGATAGTGTTAATCCACTTTCCGAAGATAATAGTACAAAGACTTTGAGACGACATGTAGAGGATGATGAGGAAAGATTTCAAGCTGATCTTAAGAAGGCTGTACGCCAGAGCCTTGATGCATTTCAAGAACAGCAGAAATTACCCTTGATTACAAATTCCAGGACACCGTTGATCAGTTATGGTGAAGTAGATAGTAATGGTCTTCCATCAAATGAACTCAATGTTGAAAATGTGGGAGCAGATATATTTGGTACAGGACTTAAGAATGAAGTTGGTGAATATAATTGCTTTCTGAATGTGATTATTCAATCCTTATGGCATTTAAGACGGTTTCGAGAAGAGTTCCTGAGAAGATCTATGACTGAGCATGTTCATGTTGGGGATCCCTGTGTTGTTTGTGCATTATATGAAATATTCGCTGCCTTGAGCATGGCATCTGCAGATGCTAGAAGAGAAGCAGTCGCCCCCACTTCTTTGAGAATAGCTCTGAGCAATCTTTGTCCTGATAGTAATTTCTTCCAAGAGGGCCAGATGAATGACGCCTCTGAAGTGCTGGCAGTCATATTTGATTGCCTACACCAGTCATTGACTCCTAGTTCCAGCGTTTCTGATACAGAATCAGTTGAAAGCAATTGCATGGGGTCTTGGGACTGTGCAAGTGCAACTTGTTTGGTACATTCCATCTTCGGAATGGACATTTTTGAACGAATGAATTGCTATAGTTGTGGCCTGGAATCTAGACATCTGAAGTACACTTCCTTCTTTCACAATATAAATGCAAGTGCCCTTCGAACAATGAAGGTTATGTGTACAGAAAGTTCCTTTGATGAACTTCTGAATGTTGTTGAAATGAATCATCAGCTAGCTTGTGATTTGGATGTTGGTGGTTGTGGAAAGCTCAATTACATTCATCACTTTCTCTCTGCACCGCCACACGTGTTCACAACTGTCTTGGGCTGGCAGAACACTTGTGAGAGTGCTGATGACATCACAGCTACATTAGCTGCTCTAAGTACTGAGATAGATATCAGCGTTCTTTATCGGGGTCTAGATCCAAAGCACACCCATAATTTGGTATCAGTGGTTTGCTATTATGGTCAACACTATCACTGTTTTGCCTACAGTCACGACAAAAAATGCTGGATAATGTATGATGATAGAACTGTTAAGGTAATTGGTGGCTGGCCTGACGTTCTTACCATGTGTGAAAAAGGACACTTGCAACCTCAGGTTCTTTTCTTTGAAGCTGTAAATTAG

Protein sequence

MGRKKKVIDPRSKQSPAARGVAVDVPSLANAVDHSSIPDKASNHNQSRIEPVFVNESDGLSYSSIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIIDDLNTKQRHLKNAIESARKAVELSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGIENPIDPAKESLQDEKNQKIPTAEARITHVQTELRQLIQKSNIYSLSSWMKNLGNGEEKFRLIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSETPQMQNEGGKADRALDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSLESKKDLLKIKTSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCRCDEKFVDSESHMHHVVQEHMGNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKMLTDQRKFKDSELDEDICSQHHSESDDCFKDAWDSSPVKHRYGNSLNECNLYEKVSNNGFKTCNENQGSLVSYSLSNSFPVSDDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDELQGLASGSHLLNQGLDQTPLCICFLGASQLRKILKFLQELCQSCGIGRYSDKSTGQIEDSKSDKQPVEVKEKVVLNRDASLLILNECFLSSDIPHPNGQVAATDHMPATAKDVIANGVLSEVDPFLSWIYGGPSSGEQLASWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRETVTEFIPKSYESVLRRRRDELIEAENDVMFIGSRFELDAVTNVLKEAEALNVNQFGYEENFASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTGMQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSKKGIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLHDEAVDGDPFQVSSDGDAAEVDIAVFENGDALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKKSAQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKPSVVDQLAENDLVADLSRNGPAMQGAFSGISNVGVLENSLPSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNVTFDNQLNEQVRYHDNLLVDSVNPLSEDNSTKTLRRHVEDDEERFQADLKKAVRQSLDAFQEQQKLPLITNSRTPLISYGEVDSNGLPSNELNVENVGADIFGTGLKNEVGEYNCFLNVIIQSLWHLRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADARREAVAPTSLRIALSNLCPDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASATCLVHSIFGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQLACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYRGLDPKHTHNLVSVVCYYGQHYHCFAYSHDKKCWIMYDDRTVKVIGGWPDVLTMCEKGHLQPQVLFFEAVN
Homology
BLAST of Spg021901 vs. NCBI nr
Match: XP_022135262.1 (uncharacterized protein LOC111007268 isoform X2 [Momordica charantia])

HSP 1 Score: 2895.5 bits (7505), Expect = 0.0e+00
Identity = 1482/1625 (91.20%), Postives = 1540/1625 (94.77%), Query Frame = 0

Query: 1    MGRKKKVIDPRSKQSPAARGVAVDVPSLANAVDHSSIPDKASNHNQSRIEPVFVNESDGL 60
            MGRKKK++DPRSKQSPAARGVAVDVPS+AN+ D SS+PDK+SNHNQ+RIE VF NESDGL
Sbjct: 1    MGRKKKIVDPRSKQSPAARGVAVDVPSVANS-DQSSVPDKSSNHNQNRIESVFGNESDGL 60

Query: 61   SYSSIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120
            SYS+IKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID
Sbjct: 61   SYSTIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120

Query: 121  DLNTKQRHLKNAIESARKAVELSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180
            DLNTKQRHLKNAIESARKAVELSP+SIEFSHFYANLLYEVANDAKEYEEVVQECERALGI
Sbjct: 121  DLNTKQRHLKNAIESARKAVELSPSSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180

Query: 181  ENPIDPAKESLQDEKNQKIPTAEARITHVQTELRQLIQKSNIYSLSSWMKNLGNGEEKFR 240
            ENPIDPAKESLQDEKNQKIPTAEARI HVQTELRQLIQKSNIYS+SSWMKNLGNGEEKFR
Sbjct: 181  ENPIDPAKESLQDEKNQKIPTAEARIAHVQTELRQLIQKSNIYSISSWMKNLGNGEEKFR 240

Query: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSETPQMQN 300
            LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKS+TPQMQN
Sbjct: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSDTPQMQN 300

Query: 301  EGGKADRALDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSLESKKDLLKIK 360
            EGGKADR LDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMS+ESKKD LKIK
Sbjct: 301  EGGKADRTLDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSMESKKDFLKIK 360

Query: 361  TSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCRCDEKFVDSESHMHHVVQEHM 420
             SDL TH SSSKDASANEVISEALSF DANKTWKFW CCRCDEKFVDSESHMHHVVQEHM
Sbjct: 361  ISDLNTHISSSKDASANEVISEALSFCDANKTWKFWVCCRCDEKFVDSESHMHHVVQEHM 420

Query: 421  GNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKMLTDQRKFKDSELDEDICSQHH 480
            GNLLPKMQSILPQNVDNEW EMLLNCSWKALDVSAAAKMLTDQ K KDSEL EDIC QHH
Sbjct: 421  GNLLPKMQSILPQNVDNEWAEMLLNCSWKALDVSAAAKMLTDQTKCKDSELVEDICLQHH 480

Query: 481  SESDDCFKDAWDSSPVKHRYGNSLNECNLYEKVSNNGFKTCNENQGSLVSYSLSNSFPVS 540
            S+SDDCFKDAWDSSP KH YGN LNECN+YEKV NNGFK C+ENQGS+ SYSLS+SFPVS
Sbjct: 481  SDSDDCFKDAWDSSPEKHNYGNGLNECNVYEKVGNNGFKACHENQGSVTSYSLSDSFPVS 540

Query: 541  DDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDELQGLASGSHLLNQGLDQTPL 600
            DDSERTKLLEKIHA+FELLIRHKYLAASHLNKVIQFTMDELQGL SG HLLNQGLDQTPL
Sbjct: 541  DDSERTKLLEKIHAIFELLIRHKYLAASHLNKVIQFTMDELQGL-SGYHLLNQGLDQTPL 600

Query: 601  CICFLGASQLRKILKFLQELCQSCGIGRYSDKSTGQIEDSKSDKQPVEVKEKVVLNRDAS 660
            CICFLGASQLRKI KFLQEL  SCG GRY DKSTGQIEDSK+DKQ VEVKEK+VLN DAS
Sbjct: 601  CICFLGASQLRKIFKFLQELSHSCGFGRYVDKSTGQIEDSKNDKQSVEVKEKIVLNEDAS 660

Query: 661  LLILNECFLSSDIPHPNGQVAATDHMPA-TAKDVIANGVLSEVDPFLSWIYGGPSSGEQL 720
            LL+LNECFLSSD  H NGQ AATDH+P  TAKDV ANGV S+ DPFLSWIY G SSGEQL
Sbjct: 661  LLLLNECFLSSDFTHANGQAAATDHIPVPTAKDVTANGVFSDADPFLSWIYAGLSSGEQL 720

Query: 721  ASWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE 780
            +SWAQ+KEEKIQ+ MEI QMLEKEF HLQSLCERKCEHL+YEEALQ +EDLCLEEGKKRE
Sbjct: 721  SSWAQTKEEKIQQGMEILQMLEKEFLHLQSLCERKCEHLSYEEALQLLEDLCLEEGKKRE 780

Query: 781  TVTEFIPKSYESVLRRRRDELIEAENDVMFIGSRFELDAVTNVLKEAEALNVNQFGYEEN 840
             VTEFIPKSYESVLR+RR+ELI+ ENDVMF+ SRFELDA+TNVLKEAEALNVNQ+GY E 
Sbjct: 781  AVTEFIPKSYESVLRKRREELIDVENDVMFL-SRFELDAITNVLKEAEALNVNQYGYGET 840

Query: 841  FASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900
            F+S+P  LCDLESGEDEDWR KDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG
Sbjct: 841  FSSMPSQLCDLESGEDEDWRTKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900

Query: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK 960
            MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK
Sbjct: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK 960

Query: 961  KGIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLHDEAVDGDPFQVSSDGD 1020
            KG+RGG+DN RHAHEKPKEKKR+KEFRK KDSKVVSGPEQD LHDE VDGDPFQV+SDGD
Sbjct: 961  KGMRGGSDNLRHAHEKPKEKKRNKEFRKAKDSKVVSGPEQDALHDEDVDGDPFQVASDGD 1020

Query: 1021 AAEVDIAVFENGDALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080
            AAEVDIAVFENG+ALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK
Sbjct: 1021 AAEVDIAVFENGNALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080

Query: 1081 SAQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKPSVVDQLAENDLVADLSRNGPAMQGAF 1140
            SAQTN EK +AVA+HDNL+GLVP+VEGADE+ K SVVDQLAENDLV DLSRNGPAM  A 
Sbjct: 1081 SAQTNPEKKVAVAIHDNLLGLVPDVEGADEHFKLSVVDQLAENDLVPDLSRNGPAMSHAP 1140

Query: 1141 SGISNVGVLENSLPSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNVTFDNQLNEQVRYH 1200
            SG+SNVGVLEN L SSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDN  FD++LNE VRYH
Sbjct: 1141 SGVSNVGVLENCLSSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNAAFDSRLNETVRYH 1200

Query: 1201 DNLLVDSVNPLSEDNSTKTLRR-HVEDDEERFQADLKKAVRQSLDAFQEQQKLPLITNSR 1260
             N L DSVNPLSEDNS KTLR+ HVEDDEERFQADLKKAVRQSLD FQEQQKLPLI++SR
Sbjct: 1201 SNFLADSVNPLSEDNSKKTLRQHHVEDDEERFQADLKKAVRQSLDTFQEQQKLPLISSSR 1260

Query: 1261 TPLISYGEVDSNGLPSNELNVENV-GADIFGTGLKNEVGEYNCFLNVIIQSLWHLRRFRE 1320
             PLIS G+VDSNG+PSNELNVENV GAD+FGTGLKNEVGEYNCFLNVIIQSLWHLRRFRE
Sbjct: 1261 IPLISCGKVDSNGVPSNELNVENVQGADMFGTGLKNEVGEYNCFLNVIIQSLWHLRRFRE 1320

Query: 1321 EFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADARREAVAPTSLRIALSNLCPDSNFF 1380
            EFLRRSMTEHVHVGDPCVVCALYEIFAALSMASAD RREAVAPTSLRIALSNLCPDSNFF
Sbjct: 1321 EFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADVRREAVAPTSLRIALSNLCPDSNFF 1380

Query: 1381 QEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASATCLVHSIFGMDI 1440
            QEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCAS TCLVHSIFGMDI
Sbjct: 1381 QEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASDTCLVHSIFGMDI 1440

Query: 1441 FERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQLACDLD 1500
            FERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQLACDLD
Sbjct: 1441 FERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQLACDLD 1500

Query: 1501 VGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYRGLDPK 1560
            VGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYRGLDPK
Sbjct: 1501 VGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYRGLDPK 1560

Query: 1561 HTHNLVSVVCYYGQHYHCFAYSHDKKCWIMYDDRTVKVIGGWPDVLTMCEKGHLQPQVLF 1620
            +THNLVSVVCYYGQHYHCFAYS+DKKCWIMYDDRTVKVIG W DVLTMCE+GHLQPQVLF
Sbjct: 1561 NTHNLVSVVCYYGQHYHCFAYSNDKKCWIMYDDRTVKVIGDWADVLTMCERGHLQPQVLF 1620

Query: 1621 FEAVN 1623
            FEAVN
Sbjct: 1621 FEAVN 1622

BLAST of Spg021901 vs. NCBI nr
Match: XP_022135261.1 (uncharacterized protein LOC111007268 isoform X1 [Momordica charantia])

HSP 1 Score: 2890.5 bits (7492), Expect = 0.0e+00
Identity = 1482/1631 (90.86%), Postives = 1540/1631 (94.42%), Query Frame = 0

Query: 1    MGRKKKVIDPRSKQSPAARGVAVDVPSLANAVDHSSIPDKASNHNQSRIEPVFVNESDGL 60
            MGRKKK++DPRSKQSPAARGVAVDVPS+AN+ D SS+PDK+SNHNQ+RIE VF NESDGL
Sbjct: 1    MGRKKKIVDPRSKQSPAARGVAVDVPSVANS-DQSSVPDKSSNHNQNRIESVFGNESDGL 60

Query: 61   SYSSIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120
            SYS+IKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID
Sbjct: 61   SYSTIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120

Query: 121  DLNTKQRHLKNAIESARKAVELSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180
            DLNTKQRHLKNAIESARKAVELSP+SIEFSHFYANLLYEVANDAKEYEEVVQECERALGI
Sbjct: 121  DLNTKQRHLKNAIESARKAVELSPSSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180

Query: 181  ENPIDPAKESLQDEKNQKIPTAEARITHVQTELRQLIQKSNIYSLSSWMKNLGNGEEKFR 240
            ENPIDPAKESLQDEKNQKIPTAEARI HVQTELRQLIQKSNIYS+SSWMKNLGNGEEKFR
Sbjct: 181  ENPIDPAKESLQDEKNQKIPTAEARIAHVQTELRQLIQKSNIYSISSWMKNLGNGEEKFR 240

Query: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSETPQMQN 300
            LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKS+TPQMQN
Sbjct: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSDTPQMQN 300

Query: 301  EGGKADRALDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSLESKKDLLKIK 360
            EGGKADR LDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMS+ESKKD LKIK
Sbjct: 301  EGGKADRTLDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSMESKKDFLKIK 360

Query: 361  TSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCRCDEKFVDSESHMHHVVQEHM 420
             SDL TH SSSKDASANEVISEALSF DANKTWKFW CCRCDEKFVDSESHMHHVVQEHM
Sbjct: 361  ISDLNTHISSSKDASANEVISEALSFCDANKTWKFWVCCRCDEKFVDSESHMHHVVQEHM 420

Query: 421  GNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKMLTDQRKFKDSELDEDICSQHH 480
            GNLLPKMQSILPQNVDNEW EMLLNCSWKALDVSAAAKMLTDQ K KDSEL EDIC QHH
Sbjct: 421  GNLLPKMQSILPQNVDNEWAEMLLNCSWKALDVSAAAKMLTDQTKCKDSELVEDICLQHH 480

Query: 481  SESDDCFKDAWDSSPVKHRYGNSLNECNLYEKVSNNGFKTCNENQGSLVSYSLSNSFPVS 540
            S+SDDCFKDAWDSSP KH YGN LNECN+YEKV NNGFK C+ENQGS+ SYSLS+SFPVS
Sbjct: 481  SDSDDCFKDAWDSSPEKHNYGNGLNECNVYEKVGNNGFKACHENQGSVTSYSLSDSFPVS 540

Query: 541  DDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDELQGLASGSHLLNQGLDQTPL 600
            DDSERTKLLEKIHA+FELLIRHKYLAASHLNKVIQFTMDELQGL SG HLLNQGLDQTPL
Sbjct: 541  DDSERTKLLEKIHAIFELLIRHKYLAASHLNKVIQFTMDELQGL-SGYHLLNQGLDQTPL 600

Query: 601  CICFLGASQLRKILKFLQELCQSCGIGRYSDKSTGQIEDSKSDKQPVEVKEKVVLNRDAS 660
            CICFLGASQLRKI KFLQEL  SCG GRY DKSTGQIEDSK+DKQ VEVKEK+VLN DAS
Sbjct: 601  CICFLGASQLRKIFKFLQELSHSCGFGRYVDKSTGQIEDSKNDKQSVEVKEKIVLNEDAS 660

Query: 661  LLILNECFLSSDIPHPNGQVAATDHMPA-TAKDVIANGVLSEVDPFLSWIYGGPSSGEQL 720
            LL+LNECFLSSD  H NGQ AATDH+P  TAKDV ANGV S+ DPFLSWIY G SSGEQL
Sbjct: 661  LLLLNECFLSSDFTHANGQAAATDHIPVPTAKDVTANGVFSDADPFLSWIYAGLSSGEQL 720

Query: 721  ASWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE 780
            +SWAQ+KEEKIQ+ MEI QMLEKEF HLQSLCERKCEHL+YEEALQ +EDLCLEEGKKRE
Sbjct: 721  SSWAQTKEEKIQQGMEILQMLEKEFLHLQSLCERKCEHLSYEEALQLLEDLCLEEGKKRE 780

Query: 781  TVTEFIPKSYESVLRRRRDELIEAENDVMFIGSRFELDAVTNVLKEAEALNVNQFGYEEN 840
             VTEFIPKSYESVLR+RR+ELI+ ENDVMF+ SRFELDA+TNVLKEAEALNVNQ+GY E 
Sbjct: 781  AVTEFIPKSYESVLRKRREELIDVENDVMFL-SRFELDAITNVLKEAEALNVNQYGYGET 840

Query: 841  FASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900
            F+S+P  LCDLESGEDEDWR KDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG
Sbjct: 841  FSSMPSQLCDLESGEDEDWRTKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900

Query: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK 960
            MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK
Sbjct: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK 960

Query: 961  KGIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLHDEAVDGDPFQVSSDGD 1020
            KG+RGG+DN RHAHEKPKEKKR+KEFRK KDSKVVSGPEQD LHDE VDGDPFQV+SDGD
Sbjct: 961  KGMRGGSDNLRHAHEKPKEKKRNKEFRKAKDSKVVSGPEQDALHDEDVDGDPFQVASDGD 1020

Query: 1021 AAEVDIAVFENGDALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080
            AAEVDIAVFENG+ALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK
Sbjct: 1021 AAEVDIAVFENGNALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080

Query: 1081 SAQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKPSVVDQLAENDLVADLSRNGPAMQGA- 1140
            SAQTN EK +AVA+HDNL+GLVP+VEGADE+ K SVVDQLAENDLV DLSRNGPAM  A 
Sbjct: 1081 SAQTNPEKKVAVAIHDNLLGLVPDVEGADEHFKLSVVDQLAENDLVPDLSRNGPAMSHAP 1140

Query: 1141 -----FSGISNVGVLENSLPSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNVTFDNQLN 1200
                  SG+SNVGVLEN L SSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDN  FD++LN
Sbjct: 1141 SGDQKISGVSNVGVLENCLSSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNAAFDSRLN 1200

Query: 1201 EQVRYHDNLLVDSVNPLSEDNSTKTLRR-HVEDDEERFQADLKKAVRQSLDAFQEQQKLP 1260
            E VRYH N L DSVNPLSEDNS KTLR+ HVEDDEERFQADLKKAVRQSLD FQEQQKLP
Sbjct: 1201 ETVRYHSNFLADSVNPLSEDNSKKTLRQHHVEDDEERFQADLKKAVRQSLDTFQEQQKLP 1260

Query: 1261 LITNSRTPLISYGEVDSNGLPSNELNVENV-GADIFGTGLKNEVGEYNCFLNVIIQSLWH 1320
            LI++SR PLIS G+VDSNG+PSNELNVENV GAD+FGTGLKNEVGEYNCFLNVIIQSLWH
Sbjct: 1261 LISSSRIPLISCGKVDSNGVPSNELNVENVQGADMFGTGLKNEVGEYNCFLNVIIQSLWH 1320

Query: 1321 LRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADARREAVAPTSLRIALSNLC 1380
            LRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASAD RREAVAPTSLRIALSNLC
Sbjct: 1321 LRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADVRREAVAPTSLRIALSNLC 1380

Query: 1381 PDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASATCLVHS 1440
            PDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCAS TCLVHS
Sbjct: 1381 PDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASDTCLVHS 1440

Query: 1441 IFGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQ 1500
            IFGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQ
Sbjct: 1441 IFGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQ 1500

Query: 1501 LACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLY 1560
            LACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLY
Sbjct: 1501 LACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLY 1560

Query: 1561 RGLDPKHTHNLVSVVCYYGQHYHCFAYSHDKKCWIMYDDRTVKVIGGWPDVLTMCEKGHL 1620
            RGLDPK+THNLVSVVCYYGQHYHCFAYS+DKKCWIMYDDRTVKVIG W DVLTMCE+GHL
Sbjct: 1561 RGLDPKNTHNLVSVVCYYGQHYHCFAYSNDKKCWIMYDDRTVKVIGDWADVLTMCERGHL 1620

Query: 1621 QPQVLFFEAVN 1623
            QPQVLFFEAVN
Sbjct: 1621 QPQVLFFEAVN 1628

BLAST of Spg021901 vs. NCBI nr
Match: XP_022135263.1 (uncharacterized protein LOC111007268 isoform X3 [Momordica charantia])

HSP 1 Score: 2822.0 bits (7314), Expect = 0.0e+00
Identity = 1456/1630 (89.33%), Postives = 1512/1630 (92.76%), Query Frame = 0

Query: 1    MGRKKKVIDPRSKQSPAARGVAVDVPSLANAVDHSSIPDKASNHNQSRIEPVFVNESDGL 60
            MGRKKK++DPRSKQSPAARGVAVDVPS+AN+ D SS+PDK+SNHNQ+RIE VF NESDGL
Sbjct: 1    MGRKKKIVDPRSKQSPAARGVAVDVPSVANS-DQSSVPDKSSNHNQNRIESVFGNESDGL 60

Query: 61   SYSSIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120
            SYS+IKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID
Sbjct: 61   SYSTIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120

Query: 121  DLNTKQRHLKNAIESARKAVELSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180
            DLNTKQRHLKNAIESARKAVELSP+SIEFSHFYANLLYEVANDAKEYEEVVQECERALGI
Sbjct: 121  DLNTKQRHLKNAIESARKAVELSPSSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180

Query: 181  ENPIDPAKESLQDEKNQKIPTAEARITHVQTELRQLIQKSNIYSLSSWMKNLGNGEEKFR 240
            ENPIDPAKESLQDEKNQKIPTAEARI HVQTELRQLIQKSNIYS+SSWMKNLGNGEEKFR
Sbjct: 181  ENPIDPAKESLQDEKNQKIPTAEARIAHVQTELRQLIQKSNIYSISSWMKNLGNGEEKFR 240

Query: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSETPQMQN 300
            LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKS+TPQMQN
Sbjct: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSDTPQMQN 300

Query: 301  EGGKADRALDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSLESKKDLLKIK 360
            EGGKADR LDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMS+ESKKD LKIK
Sbjct: 301  EGGKADRTLDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSMESKKDFLKIK 360

Query: 361  TSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCRCDEKFVDSESHMHHVVQEHM 420
             SDL TH SSSKDASANEVISEALSF DANKTWKFW CCRCDEKFVDSESHMHHVVQEHM
Sbjct: 361  ISDLNTHISSSKDASANEVISEALSFCDANKTWKFWVCCRCDEKFVDSESHMHHVVQEHM 420

Query: 421  GNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKMLTDQRKFKDSELDEDICSQHH 480
            GNLLPKMQSILPQNVDNEW EMLLNCSWKALDVSAAAKMLTDQ K KDSEL EDIC QHH
Sbjct: 421  GNLLPKMQSILPQNVDNEWAEMLLNCSWKALDVSAAAKMLTDQTKCKDSELVEDICLQHH 480

Query: 481  SESDDCFKDAWDSSPVKHRYGNSLNECNLYEKVSNNGFKTCNENQGSLVSYSLSNSFPVS 540
            S+SDDCFKDAWDSSP KH YGN LNECN+YEKV NNGFK C+ENQGS+ SYSLS+SFPVS
Sbjct: 481  SDSDDCFKDAWDSSPEKHNYGNGLNECNVYEKVGNNGFKACHENQGSVTSYSLSDSFPVS 540

Query: 541  DDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDELQGLASGSHLLNQGLDQTPL 600
            DDSERTKLLEKIHA+FELLIRHKYLAASHLNKVIQFTMDELQGL SG HLLNQGLDQTPL
Sbjct: 541  DDSERTKLLEKIHAIFELLIRHKYLAASHLNKVIQFTMDELQGL-SGYHLLNQGLDQTPL 600

Query: 601  CICFLGASQLRKILKFLQELCQSCGIGRYSDKSTGQIEDSKSDKQPVEVKEKVVLNRDAS 660
            CICFLGASQLRKI KFLQEL  SCG GRY DKSTGQIEDSK+DKQ VEVKEK+VLN DAS
Sbjct: 601  CICFLGASQLRKIFKFLQELSHSCGFGRYVDKSTGQIEDSKNDKQSVEVKEKIVLNEDAS 660

Query: 661  LLILNECFLSSDIPHPNGQVAATDHMPATAKDVIANGVLSEVDPFLSWIYGGPSSGEQLA 720
            LL+LNECFLSSD  H NG                                   SSGEQL+
Sbjct: 661  LLLLNECFLSSDFTHANGL----------------------------------SSGEQLS 720

Query: 721  SWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRET 780
            SWAQ+KEEKIQ+ MEI QMLEKEF HLQSLCERKCEHL+YEEALQ +EDLCLEEGKKRE 
Sbjct: 721  SWAQTKEEKIQQGMEILQMLEKEFLHLQSLCERKCEHLSYEEALQLLEDLCLEEGKKREA 780

Query: 781  VTEFIPKSYESVLRRRRDELIEAENDVMFIGSRFELDAVTNVLKEAEALNVNQFGYEENF 840
            VTEFIPKSYESVLR+RR+ELI+ ENDVMF+ SRFELDA+TNVLKEAEALNVNQ+GY E F
Sbjct: 781  VTEFIPKSYESVLRKRREELIDVENDVMFL-SRFELDAITNVLKEAEALNVNQYGYGETF 840

Query: 841  ASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTGM 900
            +S+P  LCDLESGEDEDWR KDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTGM
Sbjct: 841  SSMPSQLCDLESGEDEDWRTKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTGM 900

Query: 901  QQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSKK 960
            QQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSKK
Sbjct: 901  QQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSKK 960

Query: 961  GIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLHDEAVDGDPFQVSSDGDA 1020
            G+RGG+DN RHAHEKPKEKKR+KEFRK KDSKVVSGPEQD LHDE VDGDPFQV+SDGDA
Sbjct: 961  GMRGGSDNLRHAHEKPKEKKRNKEFRKAKDSKVVSGPEQDALHDEDVDGDPFQVASDGDA 1020

Query: 1021 AEVDIAVFENGDALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKKS 1080
            AEVDIAVFENG+ALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKKS
Sbjct: 1021 AEVDIAVFENGNALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKKS 1080

Query: 1081 AQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKPSVVDQLAENDLVADLSRNGPAMQGA-- 1140
            AQTN EK +AVA+HDNL+GLVP+VEGADE+ K SVVDQLAENDLV DLSRNGPAM  A  
Sbjct: 1081 AQTNPEKKVAVAIHDNLLGLVPDVEGADEHFKLSVVDQLAENDLVPDLSRNGPAMSHAPS 1140

Query: 1141 ----FSGISNVGVLENSLPSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNVTFDNQLNE 1200
                 SG+SNVGVLEN L SSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDN  FD++LNE
Sbjct: 1141 GDQKISGVSNVGVLENCLSSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNAAFDSRLNE 1200

Query: 1201 QVRYHDNLLVDSVNPLSEDNSTKTLRR-HVEDDEERFQADLKKAVRQSLDAFQEQQKLPL 1260
             VRYH N L DSVNPLSEDNS KTLR+ HVEDDEERFQADLKKAVRQSLD FQEQQKLPL
Sbjct: 1201 TVRYHSNFLADSVNPLSEDNSKKTLRQHHVEDDEERFQADLKKAVRQSLDTFQEQQKLPL 1260

Query: 1261 ITNSRTPLISYGEVDSNGLPSNELNVENV-GADIFGTGLKNEVGEYNCFLNVIIQSLWHL 1320
            I++SR PLIS G+VDSNG+PSNELNVENV GAD+FGTGLKNEVGEYNCFLNVIIQSLWHL
Sbjct: 1261 ISSSRIPLISCGKVDSNGVPSNELNVENVQGADMFGTGLKNEVGEYNCFLNVIIQSLWHL 1320

Query: 1321 RRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADARREAVAPTSLRIALSNLCP 1380
            RRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASAD RREAVAPTSLRIALSNLCP
Sbjct: 1321 RRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADVRREAVAPTSLRIALSNLCP 1380

Query: 1381 DSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASATCLVHSI 1440
            DSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCAS TCLVHSI
Sbjct: 1381 DSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASDTCLVHSI 1440

Query: 1441 FGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQL 1500
            FGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQL
Sbjct: 1441 FGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQL 1500

Query: 1501 ACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYR 1560
            ACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYR
Sbjct: 1501 ACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYR 1560

Query: 1561 GLDPKHTHNLVSVVCYYGQHYHCFAYSHDKKCWIMYDDRTVKVIGGWPDVLTMCEKGHLQ 1620
            GLDPK+THNLVSVVCYYGQHYHCFAYS+DKKCWIMYDDRTVKVIG W DVLTMCE+GHLQ
Sbjct: 1561 GLDPKNTHNLVSVVCYYGQHYHCFAYSNDKKCWIMYDDRTVKVIGDWADVLTMCERGHLQ 1593

Query: 1621 PQVLFFEAVN 1623
            PQVLFFEAVN
Sbjct: 1621 PQVLFFEAVN 1593

BLAST of Spg021901 vs. NCBI nr
Match: XP_022972240.1 (uncharacterized protein LOC111470828 [Cucurbita maxima])

HSP 1 Score: 2805.8 bits (7272), Expect = 0.0e+00
Identity = 1444/1624 (88.92%), Postives = 1513/1624 (93.17%), Query Frame = 0

Query: 1    MGRKKKVIDPRSKQSPAARGVAVDVPSLANAVDHSSIPDKASNHNQSRIEPVFVNESDGL 60
            MG KKK+IDPRSKQSPAARGV VDVPS ANA D +SIP+KASNHNQSRIEPV +N  DGL
Sbjct: 1    MGPKKKIIDPRSKQSPAARGVVVDVPSSANAADQNSIPEKASNHNQSRIEPVLLNGPDGL 60

Query: 61   SYSSIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120
            SYS+IKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID
Sbjct: 61   SYSTIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120

Query: 121  DLNTKQRHLKNAIESARKAVELSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180
            DLNTKQRHLKNAIESARKAV+LSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI
Sbjct: 121  DLNTKQRHLKNAIESARKAVQLSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180

Query: 181  ENPIDPAKESLQDEKNQKIPTAEARITHVQTELRQLIQKSNIYSLSSWMKNLGNGEEKFR 240
            ENPIDPAKESLQDEK+QKIPTAEARITHVQTELRQLIQKSNIYS+SSWMKNLGNGEEKFR
Sbjct: 181  ENPIDPAKESLQDEKSQKIPTAEARITHVQTELRQLIQKSNIYSISSWMKNLGNGEEKFR 240

Query: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSETPQMQN 300
            LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQK ETPQ+QN
Sbjct: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKCETPQIQN 300

Query: 301  EGGKADRALDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSLESKKDLLKIK 360
            E GK DR+LDSSSGSGTRVGERRKHGNLRK GSSAERRD VHSFWNSMS+E+KKDL KIK
Sbjct: 301  EEGKVDRSLDSSSGSGTRVGERRKHGNLRKQGSSAERRDLVHSFWNSMSIEAKKDLFKIK 360

Query: 361  TSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCRCDEKFVDSESHMHHVVQEHM 420
             S+LK HFSSSKDASANEVISEALSFYDANKTWKFW CCRCDEKFVDS+SHMHHVVQEHM
Sbjct: 361  ISNLKAHFSSSKDASANEVISEALSFYDANKTWKFWVCCRCDEKFVDSDSHMHHVVQEHM 420

Query: 421  GNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKMLTDQRKFKDSELDEDICSQHH 480
            GNLLPKMQSILPQNVD+EWT+M+LNCSWKALDVSAAAKMLT + K KDSEL  DI  QHH
Sbjct: 421  GNLLPKMQSILPQNVDDEWTKMILNCSWKALDVSAAAKMLTTETKCKDSELVVDIFPQHH 480

Query: 481  SESDDCFKDAWDSSPVKHRYGNSLNECNLYEKVSNNGFKTCNENQGSLVSYSLSNSFPVS 540
            SE D+ FKDAWDSSP KH+YG+SLNEC L EK++NNG  T +ENQGS+ SYS S+SFPVS
Sbjct: 481  SECDNYFKDAWDSSPEKHKYGDSLNECRLSEKITNNGSMTYHENQGSVASYSSSDSFPVS 540

Query: 541  DDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDELQGLASGSHLLNQGLDQTPL 600
            DD ERTKLLEKIHAVFELLIRHKYLAASHLNKVIQF +DELQGLASGSHLLNQGLDQTPL
Sbjct: 541  DDPERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFAIDELQGLASGSHLLNQGLDQTPL 600

Query: 601  CICFLGASQLRKILKFLQELCQSCGIGRYSDKSTGQIEDSKSDKQPVEVKEKVVLNRDAS 660
            CICFLGASQLRKILKFLQEL QSCGIGRYSDK TGQIEDSKSDKQ VEV+EKVVL+ D S
Sbjct: 601  CICFLGASQLRKILKFLQELSQSCGIGRYSDKITGQIEDSKSDKQAVEVREKVVLSEDGS 660

Query: 661  LLILNECFLSSDIPHPNGQVAATDHM-PATAKDVIANGVLSEVDPFLSWIYGGPSSGEQL 720
            LL+LN+C LSSDI H +  VAA  HM  ATAKD+ +NGVLS+VDPFLSWIY GPSSGEQL
Sbjct: 661  LLLLNKCLLSSDITHADVPVAAAYHMLAATAKDLTSNGVLSDVDPFLSWIYAGPSSGEQL 720

Query: 721  ASWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE 780
             SW ++KEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE
Sbjct: 721  VSWEKTKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE 780

Query: 781  TVTEFIPKSYESVLRRRRDELIEAENDVMFIGSRFELDAVTNVLKEAEALNVNQFGYEEN 840
             + EFIPKSYESVLR+RR+ELIEAEN V+ I SRFELDAVTNVLKEAE LNVNQFGY+E 
Sbjct: 781  ALNEFIPKSYESVLRKRREELIEAENGVLLISSRFELDAVTNVLKEAETLNVNQFGYQET 840

Query: 841  FASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900
            FASV P L DLESGEDE+ RAKDYLH+VDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG
Sbjct: 841  FASVSPQLGDLESGEDEERRAKDYLHEVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900

Query: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK 960
            MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAA EAFLAELALDSK
Sbjct: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAASEAFLAELALDSK 960

Query: 961  KGIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLHDEAVDGDPFQVSSDGD 1020
            KGIRGG DNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVL  +AVDGDPF VSSDGD
Sbjct: 961  KGIRGGTDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLRGQAVDGDPFPVSSDGD 1020

Query: 1021 AAEVDIAVFENGDALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080
            AAEVDIAVFEN DALRLQEEE RRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK
Sbjct: 1021 AAEVDIAVFENVDALRLQEEELRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080

Query: 1081 SAQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKPSVVDQLAENDLVADLSRNGPAMQGAF 1140
            SAQT          H+NL GLVPNVEGAD++ KPSVVD+LAENDLV+DLS NGPAM+ A 
Sbjct: 1081 SAQT---------THNNLAGLVPNVEGADKHFKPSVVDKLAENDLVSDLSGNGPAMRNAL 1140

Query: 1141 SGISNVGVLENSLPSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNVTFDNQLNEQVRYH 1200
            SG+SNVGV+ENSLPSSDR+KGRKGRRHKVSIKPVDGNQPSPSDKDNV FDNQL EQVRYH
Sbjct: 1141 SGVSNVGVIENSLPSSDRKKGRKGRRHKVSIKPVDGNQPSPSDKDNVAFDNQLTEQVRYH 1200

Query: 1201 DNLLVDSVNPLSEDNSTKTLRRHVEDDEERFQADLKKAVRQSLDAFQEQQKLPLITNSRT 1260
             N L DSVNPL EDNSTKT R+HVED+EERFQADLKKAVRQSLDAFQEQQKLPLI +SRT
Sbjct: 1201 GNPLQDSVNPLLEDNSTKTSRQHVEDEEERFQADLKKAVRQSLDAFQEQQKLPLIMSSRT 1260

Query: 1261 PLISYGEVDSNGLPSNELNVENV-GADIFGTGLKNEVGEYNCFLNVIIQSLWHLRRFREE 1320
            PLIS+GEVDSNGLPSNE NVENV  A +FGTGLKNEVGEYNCFLNVIIQSLWHLRRFREE
Sbjct: 1261 PLISHGEVDSNGLPSNERNVENVQAAGMFGTGLKNEVGEYNCFLNVIIQSLWHLRRFREE 1320

Query: 1321 FLRRSMTEHVHVGDPCVVCALYEIFAALSMASADARREAVAPTSLRIALSNLCPDSNFFQ 1380
            FLRRSMTEHVHVGDPCVVCALYEIFAAL+ ASAD+RREAVAPTSLR+ALSNLCPDSNFFQ
Sbjct: 1321 FLRRSMTEHVHVGDPCVVCALYEIFAALNKASADSRREAVAPTSLRVALSNLCPDSNFFQ 1380

Query: 1381 EGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASATCLVHSIFGMDIF 1440
            EGQMNDASEVLAVIFDCLHQSLT  SSVSDT++VESNC+GSWDC+S TCLVHSIFGMDIF
Sbjct: 1381 EGQMNDASEVLAVIFDCLHQSLTAGSSVSDTDTVESNCIGSWDCSSDTCLVHSIFGMDIF 1440

Query: 1441 ERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQLACDLDV 1500
            ERMNCYSCGLESRHLKYTSFFHNINASALRT KVMC+ESSFDELLNVVEMNHQL CD DV
Sbjct: 1441 ERMNCYSCGLESRHLKYTSFFHNINASALRTTKVMCSESSFDELLNVVEMNHQLVCDQDV 1500

Query: 1501 GGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYRGLDPKH 1560
            GGCGKLNYIHHFLSAPPHVFTTVLGWQ TCESADDIT TLAALSTEID+SVLYRGLDPK+
Sbjct: 1501 GGCGKLNYIHHFLSAPPHVFTTVLGWQKTCESADDITETLAALSTEIDVSVLYRGLDPKN 1560

Query: 1561 THNLVSVVCYYGQHYHCFAYSHDKKCWIMYDDRTVKVIGGWPDVLTMCEKGHLQPQVLFF 1620
             HNLVSVVCYYGQHYHCFAYS DKKCWIMYDDRTVKVIGGW DVLTMCEKGHLQPQVLFF
Sbjct: 1561 VHNLVSVVCYYGQHYHCFAYSCDKKCWIMYDDRTVKVIGGWADVLTMCEKGHLQPQVLFF 1615

Query: 1621 EAVN 1623
            EAVN
Sbjct: 1621 EAVN 1615

BLAST of Spg021901 vs. NCBI nr
Match: KAG7021496.1 (Inactive ubiquitin carboxyl-terminal hydrolase 54, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2805.4 bits (7271), Expect = 0.0e+00
Identity = 1446/1624 (89.04%), Postives = 1513/1624 (93.17%), Query Frame = 0

Query: 1    MGRKKKVIDPRSKQSPAARGVAVDVPSLANAVDHSSIPDKASNHNQSRIEPVFVNESDGL 60
            MG KKK+IDPRSKQSPAARGV VDVPS ANA D +SIP+KASNHNQSRIEPV +N  DGL
Sbjct: 1    MGPKKKIIDPRSKQSPAARGVVVDVPSSANAADQNSIPEKASNHNQSRIEPVLLNGPDGL 60

Query: 61   SYSSIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120
            SYS+IKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID
Sbjct: 61   SYSTIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120

Query: 121  DLNTKQRHLKNAIESARKAVELSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180
            DLNTKQRHLKNAIESARKAV+LSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI
Sbjct: 121  DLNTKQRHLKNAIESARKAVQLSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180

Query: 181  ENPIDPAKESLQDEKNQKIPTAEARITHVQTELRQLIQKSNIYSLSSWMKNLGNGEEKFR 240
            ENPIDPAKESLQDEK+QKIPTAEARITHVQTELRQLIQKSNIYS+SSWMKNLGNGEEKFR
Sbjct: 181  ENPIDPAKESLQDEKSQKIPTAEARITHVQTELRQLIQKSNIYSISSWMKNLGNGEEKFR 240

Query: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSETPQMQN 300
            LIPIRRVTEDPMEVRLVQARR NEIKKATKTPEERRKEIEVRVAAARLLQQK ETPQ+QN
Sbjct: 241  LIPIRRVTEDPMEVRLVQARRTNEIKKATKTPEERRKEIEVRVAAARLLQQKCETPQIQN 300

Query: 301  EGGKADRALDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSLESKKDLLKIK 360
            E GK DR+LDSS GSGTR GERRKHGNLRK GSSAERRD VHSFWNSMS+E+KKDLLKIK
Sbjct: 301  EEGKVDRSLDSSLGSGTRAGERRKHGNLRKQGSSAERRDLVHSFWNSMSIEAKKDLLKIK 360

Query: 361  TSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCRCDEKFVDSESHMHHVVQEHM 420
             S+LK HFSSSKDASANEVISEALSFY+ANKTWKFW CCRCDEKFVDS+SHMHHVVQEHM
Sbjct: 361  ISNLKAHFSSSKDASANEVISEALSFYNANKTWKFWVCCRCDEKFVDSDSHMHHVVQEHM 420

Query: 421  GNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKMLTDQRKFKDSELDEDICSQHH 480
            GNLLPKMQSILPQNVD+EWT+M+LNCSWKALDVSAAAKMLT + K KDSEL  DI  QHH
Sbjct: 421  GNLLPKMQSILPQNVDDEWTKMILNCSWKALDVSAAAKMLTAETKCKDSELVVDIFPQHH 480

Query: 481  SESDDCFKDAWDSSPVKHRYGNSLNECNLYEKVSNNGFKTCNENQGSLVSYSLSNSFPVS 540
            SE D+ FKDAW SSP KH+YG+SLNEC L EK++NNG KT +ENQGS+ SYS S+SFPVS
Sbjct: 481  SECDNYFKDAWGSSPEKHKYGDSLNECRLSEKITNNGSKTYHENQGSVASYSSSDSFPVS 540

Query: 541  DDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDELQGLASGSHLLNQGLDQTPL 600
            DD ERTKLLEKIHAVFELLIRHKYLAASHLNKVIQF MDELQGLASGSHLLNQGLDQTPL
Sbjct: 541  DDPERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFAMDELQGLASGSHLLNQGLDQTPL 600

Query: 601  CICFLGASQLRKILKFLQELCQSCGIGRYSDKSTGQIEDSKSDKQPVEVKEKVVLNRDAS 660
            CICFLGASQLRKILKFLQEL QSCGIGRYSDKSTGQIEDS SDKQ VEV+EKVVL+ DAS
Sbjct: 601  CICFLGASQLRKILKFLQELSQSCGIGRYSDKSTGQIEDSMSDKQAVEVREKVVLSEDAS 660

Query: 661  LLILNECFLSSDIPHPNGQVAATDHM-PATAKDVIANGVLSEVDPFLSWIYGGPSSGEQL 720
            LL+LNEC LSSDI H +  VAA DHM  ATAKDV +NGVLS VDPFLSWIY GPSSGEQL
Sbjct: 661  LLLLNECLLSSDITHADVPVAAADHMLAATAKDVTSNGVLSGVDPFLSWIYAGPSSGEQL 720

Query: 721  ASWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE 780
             SW ++KEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE
Sbjct: 721  VSWEKTKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE 780

Query: 781  TVTEFIPKSYESVLRRRRDELIEAENDVMFIGSRFELDAVTNVLKEAEALNVNQFGYEEN 840
             + EFIPKSYESVLR+RR+ELIEAEN V+FI SRFELDAVTNVLKEAE LNVNQFGY+E 
Sbjct: 781  ALNEFIPKSYESVLRKRREELIEAENGVLFISSRFELDAVTNVLKEAETLNVNQFGYQET 840

Query: 841  FASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900
            FASV P L DLESG+DE+ RAKDYLH+VDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG
Sbjct: 841  FASVSPQLGDLESGQDEERRAKDYLHEVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900

Query: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK 960
            MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAA EAFLAELALDSK
Sbjct: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAASEAFLAELALDSK 960

Query: 961  KGIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLHDEAVDGDPFQVSSDGD 1020
            KGIRGG DNSRHAHEKPKEKK+SKEFRKVKDSKVVSGPEQDV   +AVDGDPF VSSDGD
Sbjct: 961  KGIRGGTDNSRHAHEKPKEKKKSKEFRKVKDSKVVSGPEQDVHRGQAVDGDPFPVSSDGD 1020

Query: 1021 AAEVDIAVFENGDALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080
            AAEVDIAVFEN DALRLQEEE RRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK
Sbjct: 1021 AAEVDIAVFENVDALRLQEEELRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080

Query: 1081 SAQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKPSVVDQLAENDLVADLSRNGPAMQGAF 1140
            SAQT           +NL GLVPNVEGAD++ KPSVVD+LAENDLV+DLSRNGPAM+ A 
Sbjct: 1081 SAQT---------TPNNLAGLVPNVEGADKHFKPSVVDKLAENDLVSDLSRNGPAMRNAL 1140

Query: 1141 SGISNVGVLENSLPSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNVTFDNQLNEQVRYH 1200
            SG+SNVGVLENSLPSSDR+KGRKGRRHKVSIKPVDGNQPSPSDKDNV FDNQL EQVRYH
Sbjct: 1141 SGVSNVGVLENSLPSSDRKKGRKGRRHKVSIKPVDGNQPSPSDKDNVAFDNQLTEQVRYH 1200

Query: 1201 DNLLVDSVNPLSEDNSTKTLRRHVEDDEERFQADLKKAVRQSLDAFQEQQKLPLITNSRT 1260
             N L DSVNPL EDNSTKT R+HVED+EERFQADLKKAVRQSLDAFQEQQKLPLI +SRT
Sbjct: 1201 GNPLQDSVNPLLEDNSTKTSRQHVEDEEERFQADLKKAVRQSLDAFQEQQKLPLIMSSRT 1260

Query: 1261 PLISYGEVDSNGLPSNELNVENV-GADIFGTGLKNEVGEYNCFLNVIIQSLWHLRRFREE 1320
            PLIS+GEVDSNGLPSNE NVENV  A +FGTGLKNEVGEYNCFLNVIIQSLWHLRRFREE
Sbjct: 1261 PLISHGEVDSNGLPSNERNVENVQAAGMFGTGLKNEVGEYNCFLNVIIQSLWHLRRFREE 1320

Query: 1321 FLRRSMTEHVHVGDPCVVCALYEIFAALSMASADARREAVAPTSLRIALSNLCPDSNFFQ 1380
            FLRRSMTEHVHVGDPCVVCALYEIFAAL+ ASAD+RREAVAPTSLR+ALSNLCPDSNFFQ
Sbjct: 1321 FLRRSMTEHVHVGDPCVVCALYEIFAALNKASADSRREAVAPTSLRVALSNLCPDSNFFQ 1380

Query: 1381 EGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASATCLVHSIFGMDIF 1440
            EGQMNDASEVLAVIFDCLHQSLT SSSVSDT+++ESNC+GSWDC+S TCLVHSIFGMDIF
Sbjct: 1381 EGQMNDASEVLAVIFDCLHQSLTASSSVSDTDTIESNCIGSWDCSSDTCLVHSIFGMDIF 1440

Query: 1441 ERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQLACDLDV 1500
            ERMNCYSCGLESRHLKYTSFFHNINASALRT KVMC+ESSFDELLNVVEMNHQL CD DV
Sbjct: 1441 ERMNCYSCGLESRHLKYTSFFHNINASALRTTKVMCSESSFDELLNVVEMNHQLVCDQDV 1500

Query: 1501 GGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYRGLDPKH 1560
            GGCGKLNYIHHFLSAPPHVFTTVLGWQ TCESADDIT TLAALSTEIDISVLYRGLDPK+
Sbjct: 1501 GGCGKLNYIHHFLSAPPHVFTTVLGWQKTCESADDITETLAALSTEIDISVLYRGLDPKN 1560

Query: 1561 THNLVSVVCYYGQHYHCFAYSHDKKCWIMYDDRTVKVIGGWPDVLTMCEKGHLQPQVLFF 1620
             HNLVSVVCYYGQHYHCFAYS DKKCWIMYDDRTVKVIGGW DVLTMCEKGHLQPQVLFF
Sbjct: 1561 VHNLVSVVCYYGQHYHCFAYSCDKKCWIMYDDRTVKVIGGWADVLTMCEKGHLQPQVLFF 1615

Query: 1621 EAVN 1623
            EAVN
Sbjct: 1621 EAVN 1615

BLAST of Spg021901 vs. ExPASy Swiss-Prot
Match: Q8BL06 (Inactive ubiquitin carboxyl-terminal hydrolase 54 OS=Mus musculus OX=10090 GN=Usp54 PE=1 SV=2)

HSP 1 Score: 157.1 bits (396), Expect = 1.6e-36
Identity = 113/343 (32.94%), Postives = 160/343 (46.65%), Query Frame = 0

Query: 1290 GLKNEVGEYNCFLNVIIQSLWHLRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMA 1349
            GL NE G+ +CFLN  +Q LWHL  FR  F  R +T H  +GD C+ CAL  IF     +
Sbjct: 32   GLSNEPGQNSCFLNSALQVLWHLDIFRRSF--RQLTTHKCMGDSCIFCALKGIFNQFQCS 91

Query: 1350 SADARREAVAPT-SLRIALSNLCPDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSD 1409
            S     E V P+ +LR AL+    D   FQ G M+DA+E    +   +H  +   +    
Sbjct: 92   S-----EKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETK--- 151

Query: 1410 TESVESNCMGSWDCASATCLVHSIFGMDIFERMNCYSCGLESRHLKYTSFFHNINASALR 1469
             E +         C +  C+ H  F M +FE+  C SCG  S  L +    H I+ +AL 
Sbjct: 152  -EDI---------CTAQHCISHQKFAMTLFEQCVCSSCGATSDPLPFIQMVHYISTTALC 211

Query: 1470 TMKVMCTE-------SSFDELL-NVVEMNHQLACDLDVGGCGKLNYIHHFLSAPPHVFTT 1529
               +   E       S F ELL N   M     C      CG+   I   L   P + T 
Sbjct: 212  NQAICMLEKREKPSPSMFGELLQNASTMGDLRNCP---SNCGERIRIRRVLMNAPQIITI 271

Query: 1530 VLGWQNTCESADDITATLAALSTEIDI-SVLYRGLDPKHTHN---LVSVVCYYGQHYHCF 1589
             L W +  E +D     + +L T + +  + +R  D +   +   LV ++CYYG+HY  F
Sbjct: 272  GLVWDS--EHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTF 331

Query: 1590 AYSHDKKCWIMYDDRTVKVIG-GWPDVLTMCEKGHLQPQVLFF 1619
             +    + W+ +DD  VK IG  W DV+T C KGH QP +L +
Sbjct: 332  FFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLY 349

BLAST of Spg021901 vs. ExPASy Swiss-Prot
Match: Q70EK8 (Inactive ubiquitin carboxyl-terminal hydrolase 53 OS=Homo sapiens OX=9606 GN=USP53 PE=1 SV=2)

HSP 1 Score: 156.8 bits (395), Expect = 2.1e-36
Identity = 110/342 (32.16%), Postives = 155/342 (45.32%), Query Frame = 0

Query: 1290 GLKNEVGEYNCFLNVIIQSLWHLRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMA 1349
            GL NE G+ +CFLN  +Q LW L  FR     R +T HV  GD C+ CAL  IFA     
Sbjct: 31   GLLNEPGQNSCFLNSAVQVLWQLDIFRRSL--RVLTGHVCQGDACIFCALKTIFAQFQ-- 90

Query: 1350 SADARREAVAPTSLRIALSNLCPDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDT 1409
               +R +A+   ++R AL+    D   FQ G M+DA+E    + + +H  + PS      
Sbjct: 91   --HSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDAD-- 150

Query: 1410 ESVESNCMGSWDCASATCLVHSIFGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRT 1469
                        C S +C+ H  F M ++E+  C SCG  S  L +T F   I+ +AL  
Sbjct: 151  -----------MCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCN 210

Query: 1470 MKVMCTE-------SSFDELLNVVEMNHQL-ACDLDVGGCGKLNYIHHFLSAPPHVFTTV 1529
                  E         F ELL           C      CG+   I   L   P + T  
Sbjct: 211  EVERMLERHERFKPEMFAELLQAANTTDDYRKCP---SNCGQKIKIRRVLMNCPEIVTIG 270

Query: 1530 LGWQNTCESADDITATLAALSTEIDI-SVLYRGLDPKHTH---NLVSVVCYYGQHYHCFA 1589
            L W +  E +D   A +  L+T + +  + YR  D    +   NLV ++CY  QHY  FA
Sbjct: 271  LVWDS--EHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFA 330

Query: 1590 YSHDKKCWIMYDDRTVKVIG-GWPDVLTMCEKGHLQPQVLFF 1619
            +      W+ +DD  VK IG  W DV++ C + H QP +LF+
Sbjct: 331  FHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFY 348

BLAST of Spg021901 vs. ExPASy Swiss-Prot
Match: Q70EL1 (Inactive ubiquitin carboxyl-terminal hydrolase 54 OS=Homo sapiens OX=9606 GN=USP54 PE=1 SV=4)

HSP 1 Score: 155.6 bits (392), Expect = 4.8e-36
Identity = 112/343 (32.65%), Postives = 159/343 (46.36%), Query Frame = 0

Query: 1290 GLKNEVGEYNCFLNVIIQSLWHLRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMA 1349
            GL NE G+ +CFLN  +Q LWHL  FR  F  R +T H  +GD C+ CAL  IF     +
Sbjct: 32   GLSNEPGQNSCFLNSALQVLWHLDIFRRSF--RQLTTHKCMGDSCIFCALKGIFNQFQCS 91

Query: 1350 SADARREAVAPT-SLRIALSNLCPDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSD 1409
            S     E V P+ +LR AL+    D   FQ G M+DA+E    +   +H  +   +    
Sbjct: 92   S-----EKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETK--- 151

Query: 1410 TESVESNCMGSWDCASATCLVHSIFGMDIFERMNCYSCGLESRHLKYTSFFHNINASALR 1469
             E +         C +  C+ H  F M +FE+  C SCG  S  L +    H I+ ++L 
Sbjct: 152  -EDI---------CTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLC 211

Query: 1470 TMKVMCTE-------SSFDELL-NVVEMNHQLACDLDVGGCGKLNYIHHFLSAPPHVFTT 1529
               +   E       S F ELL N   M     C      CG+   I   L   P + T 
Sbjct: 212  NQAICMLERREKPSPSMFGELLQNASTMGDLRNCP---SNCGERIRIRRVLMNAPQIITI 271

Query: 1530 VLGWQNT-CESADDITATLAALSTEIDISVLYRGLDPKHTHN---LVSVVCYYGQHYHCF 1589
             L W +   + A+D+  +L       D+   +R  D +   +   LV ++CYYG+HY  F
Sbjct: 272  GLVWDSDHSDLAEDVIHSLGTCLKLGDL--FFRVTDDRAKQSELYLVGMICYYGKHYSTF 331

Query: 1590 AYSHDKKCWIMYDDRTVKVIG-GWPDVLTMCEKGHLQPQVLFF 1619
             +    + W+ +DD  VK IG  W DV+T C KGH QP +L +
Sbjct: 332  FFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLY 349

BLAST of Spg021901 vs. ExPASy Swiss-Prot
Match: Q6IE24 (Inactive ubiquitin carboxyl-terminal hydrolase 54 OS=Rattus norvegicus OX=10116 GN=Usp54 PE=1 SV=2)

HSP 1 Score: 154.8 bits (390), Expect = 8.1e-36
Identity = 111/343 (32.36%), Postives = 158/343 (46.06%), Query Frame = 0

Query: 1290 GLKNEVGEYNCFLNVIIQSLWHLRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMA 1349
            GL NE G+ +CFLN  +Q LWHL  FR  F  R +T H  +GD C+ CAL  IF     +
Sbjct: 32   GLSNEPGQNSCFLNSALQVLWHLDIFRRSF--RQLTSHKCMGDSCIFCALKGIFKQFQCS 91

Query: 1350 SADARREAVAPT-SLRIALSNLCPDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSD 1409
            S     E V P+ +LR AL+    D   FQ G M+DA+E    +   +H  +   +    
Sbjct: 92   S-----EKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETK--- 151

Query: 1410 TESVESNCMGSWDCASATCLVHSIFGMDIFERMNCYSCGLESRHLKYTSFFHNINASALR 1469
             E +         C +  C+ H  F M +FE+  C SCG  S  L +    H I+ ++L 
Sbjct: 152  -EDI---------CTAPHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLC 211

Query: 1470 TMKVMCTE-------SSFDELL-NVVEMNHQLACDLDVGGCGKLNYIHHFLSAPPHVFTT 1529
               +   E         F ELL N   M     C      CG+   I   L   P + T 
Sbjct: 212  NQAICMLEKREKPSPGMFGELLQNASTMGDLRDCP---SNCGERIRIRRVLMNAPQIITI 271

Query: 1530 VLGWQNT-CESADDITATLAALSTEIDISVLYRGLDPKHTHN---LVSVVCYYGQHYHCF 1589
             L W +   + A+D+  +L       D+   +R  D +   +   LV ++CYYG+HY  F
Sbjct: 272  GLVWDSDHSDLAEDVIHSLGTCLKLGDL--FFRVTDDRAKQSELYLVGMICYYGKHYSTF 331

Query: 1590 AYSHDKKCWIMYDDRTVKVIG-GWPDVLTMCEKGHLQPQVLFF 1619
             +    + W+ +DD  VK IG  W DV+T C KGH QP +L +
Sbjct: 332  FFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLY 349

BLAST of Spg021901 vs. ExPASy Swiss-Prot
Match: P15975 (Inactive ubiquitin carboxyl-terminal hydrolase 53 OS=Mus musculus OX=10090 GN=Usp53 PE=1 SV=2)

HSP 1 Score: 150.2 bits (378), Expect = 2.0e-34
Identity = 108/342 (31.58%), Postives = 158/342 (46.20%), Query Frame = 0

Query: 1290 GLKNEVGEYNCFLNVIIQSLWHLRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMA 1349
            GL NE G+ +CFLN  +Q LW L  FR     R++T H+  GD C+ CAL  IFA     
Sbjct: 31   GLLNEPGQNSCFLNSAVQVLWQLDIFRRSL--RALTGHICQGDACIFCALKTIFAQFQ-- 90

Query: 1350 SADARREAVAPTSLRIALSNLCPDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDT 1409
               +R +A+   ++R AL+    D   FQ G M+DA+E    I   +H  L P+      
Sbjct: 91   --HSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENILARIHFHLVPNRDAD-- 150

Query: 1410 ESVESNCMGSWDCASATCLVHSIFGMDIFERMNCYSCGLESRHLKYTSFFHNINASAL-- 1469
                        C S +C+ H  F M ++E+  C SCG  S  L +T     I+ +AL  
Sbjct: 151  -----------MCTSKSCVTHQKFAMTLYEQCVCRSCGASSDPLPFTELVRYISTTALCN 210

Query: 1470 ---RTMK--VMCTESSFDELLNVVE-MNHQLACDLDVGGCGKLNYIHHFLSAPPHVFTTV 1529
               R M+         F ELL      +    C      CG+   I   L   P + T  
Sbjct: 211  EVERMMERHERVKPEMFAELLQAANTADDYRKCP---SNCGQKIKIRRVLMNCPEIVTIG 270

Query: 1530 LGWQNTCESADDITATLAALSTEIDI-SVLYRGLDPKHTH---NLVSVVCYYGQHYHCFA 1589
            L W +  E +D     + +L+T + +  + YR  D   T    +LV ++CY  +HY  FA
Sbjct: 271  LVWDS--EHSDLTEDVVRSLATHLYLPGLFYRVTDENATDSELHLVGMICYTSRHYCAFA 330

Query: 1590 YSHDKKCWIMYDDRTVKVIG-GWPDVLTMCEKGHLQPQVLFF 1619
            +      W+ +DD  VK +G  W DV++ C + HLQP +LF+
Sbjct: 331  FHTKSSKWVFFDDAHVKEMGTRWKDVVSKCIRCHLQPLLLFY 348

BLAST of Spg021901 vs. ExPASy TrEMBL
Match: A0A6J1C274 (uncharacterized protein LOC111007268 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007268 PE=4 SV=1)

HSP 1 Score: 2895.5 bits (7505), Expect = 0.0e+00
Identity = 1482/1625 (91.20%), Postives = 1540/1625 (94.77%), Query Frame = 0

Query: 1    MGRKKKVIDPRSKQSPAARGVAVDVPSLANAVDHSSIPDKASNHNQSRIEPVFVNESDGL 60
            MGRKKK++DPRSKQSPAARGVAVDVPS+AN+ D SS+PDK+SNHNQ+RIE VF NESDGL
Sbjct: 1    MGRKKKIVDPRSKQSPAARGVAVDVPSVANS-DQSSVPDKSSNHNQNRIESVFGNESDGL 60

Query: 61   SYSSIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120
            SYS+IKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID
Sbjct: 61   SYSTIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120

Query: 121  DLNTKQRHLKNAIESARKAVELSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180
            DLNTKQRHLKNAIESARKAVELSP+SIEFSHFYANLLYEVANDAKEYEEVVQECERALGI
Sbjct: 121  DLNTKQRHLKNAIESARKAVELSPSSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180

Query: 181  ENPIDPAKESLQDEKNQKIPTAEARITHVQTELRQLIQKSNIYSLSSWMKNLGNGEEKFR 240
            ENPIDPAKESLQDEKNQKIPTAEARI HVQTELRQLIQKSNIYS+SSWMKNLGNGEEKFR
Sbjct: 181  ENPIDPAKESLQDEKNQKIPTAEARIAHVQTELRQLIQKSNIYSISSWMKNLGNGEEKFR 240

Query: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSETPQMQN 300
            LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKS+TPQMQN
Sbjct: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSDTPQMQN 300

Query: 301  EGGKADRALDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSLESKKDLLKIK 360
            EGGKADR LDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMS+ESKKD LKIK
Sbjct: 301  EGGKADRTLDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSMESKKDFLKIK 360

Query: 361  TSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCRCDEKFVDSESHMHHVVQEHM 420
             SDL TH SSSKDASANEVISEALSF DANKTWKFW CCRCDEKFVDSESHMHHVVQEHM
Sbjct: 361  ISDLNTHISSSKDASANEVISEALSFCDANKTWKFWVCCRCDEKFVDSESHMHHVVQEHM 420

Query: 421  GNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKMLTDQRKFKDSELDEDICSQHH 480
            GNLLPKMQSILPQNVDNEW EMLLNCSWKALDVSAAAKMLTDQ K KDSEL EDIC QHH
Sbjct: 421  GNLLPKMQSILPQNVDNEWAEMLLNCSWKALDVSAAAKMLTDQTKCKDSELVEDICLQHH 480

Query: 481  SESDDCFKDAWDSSPVKHRYGNSLNECNLYEKVSNNGFKTCNENQGSLVSYSLSNSFPVS 540
            S+SDDCFKDAWDSSP KH YGN LNECN+YEKV NNGFK C+ENQGS+ SYSLS+SFPVS
Sbjct: 481  SDSDDCFKDAWDSSPEKHNYGNGLNECNVYEKVGNNGFKACHENQGSVTSYSLSDSFPVS 540

Query: 541  DDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDELQGLASGSHLLNQGLDQTPL 600
            DDSERTKLLEKIHA+FELLIRHKYLAASHLNKVIQFTMDELQGL SG HLLNQGLDQTPL
Sbjct: 541  DDSERTKLLEKIHAIFELLIRHKYLAASHLNKVIQFTMDELQGL-SGYHLLNQGLDQTPL 600

Query: 601  CICFLGASQLRKILKFLQELCQSCGIGRYSDKSTGQIEDSKSDKQPVEVKEKVVLNRDAS 660
            CICFLGASQLRKI KFLQEL  SCG GRY DKSTGQIEDSK+DKQ VEVKEK+VLN DAS
Sbjct: 601  CICFLGASQLRKIFKFLQELSHSCGFGRYVDKSTGQIEDSKNDKQSVEVKEKIVLNEDAS 660

Query: 661  LLILNECFLSSDIPHPNGQVAATDHMPA-TAKDVIANGVLSEVDPFLSWIYGGPSSGEQL 720
            LL+LNECFLSSD  H NGQ AATDH+P  TAKDV ANGV S+ DPFLSWIY G SSGEQL
Sbjct: 661  LLLLNECFLSSDFTHANGQAAATDHIPVPTAKDVTANGVFSDADPFLSWIYAGLSSGEQL 720

Query: 721  ASWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE 780
            +SWAQ+KEEKIQ+ MEI QMLEKEF HLQSLCERKCEHL+YEEALQ +EDLCLEEGKKRE
Sbjct: 721  SSWAQTKEEKIQQGMEILQMLEKEFLHLQSLCERKCEHLSYEEALQLLEDLCLEEGKKRE 780

Query: 781  TVTEFIPKSYESVLRRRRDELIEAENDVMFIGSRFELDAVTNVLKEAEALNVNQFGYEEN 840
             VTEFIPKSYESVLR+RR+ELI+ ENDVMF+ SRFELDA+TNVLKEAEALNVNQ+GY E 
Sbjct: 781  AVTEFIPKSYESVLRKRREELIDVENDVMFL-SRFELDAITNVLKEAEALNVNQYGYGET 840

Query: 841  FASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900
            F+S+P  LCDLESGEDEDWR KDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG
Sbjct: 841  FSSMPSQLCDLESGEDEDWRTKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900

Query: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK 960
            MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK
Sbjct: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK 960

Query: 961  KGIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLHDEAVDGDPFQVSSDGD 1020
            KG+RGG+DN RHAHEKPKEKKR+KEFRK KDSKVVSGPEQD LHDE VDGDPFQV+SDGD
Sbjct: 961  KGMRGGSDNLRHAHEKPKEKKRNKEFRKAKDSKVVSGPEQDALHDEDVDGDPFQVASDGD 1020

Query: 1021 AAEVDIAVFENGDALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080
            AAEVDIAVFENG+ALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK
Sbjct: 1021 AAEVDIAVFENGNALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080

Query: 1081 SAQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKPSVVDQLAENDLVADLSRNGPAMQGAF 1140
            SAQTN EK +AVA+HDNL+GLVP+VEGADE+ K SVVDQLAENDLV DLSRNGPAM  A 
Sbjct: 1081 SAQTNPEKKVAVAIHDNLLGLVPDVEGADEHFKLSVVDQLAENDLVPDLSRNGPAMSHAP 1140

Query: 1141 SGISNVGVLENSLPSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNVTFDNQLNEQVRYH 1200
            SG+SNVGVLEN L SSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDN  FD++LNE VRYH
Sbjct: 1141 SGVSNVGVLENCLSSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNAAFDSRLNETVRYH 1200

Query: 1201 DNLLVDSVNPLSEDNSTKTLRR-HVEDDEERFQADLKKAVRQSLDAFQEQQKLPLITNSR 1260
             N L DSVNPLSEDNS KTLR+ HVEDDEERFQADLKKAVRQSLD FQEQQKLPLI++SR
Sbjct: 1201 SNFLADSVNPLSEDNSKKTLRQHHVEDDEERFQADLKKAVRQSLDTFQEQQKLPLISSSR 1260

Query: 1261 TPLISYGEVDSNGLPSNELNVENV-GADIFGTGLKNEVGEYNCFLNVIIQSLWHLRRFRE 1320
             PLIS G+VDSNG+PSNELNVENV GAD+FGTGLKNEVGEYNCFLNVIIQSLWHLRRFRE
Sbjct: 1261 IPLISCGKVDSNGVPSNELNVENVQGADMFGTGLKNEVGEYNCFLNVIIQSLWHLRRFRE 1320

Query: 1321 EFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADARREAVAPTSLRIALSNLCPDSNFF 1380
            EFLRRSMTEHVHVGDPCVVCALYEIFAALSMASAD RREAVAPTSLRIALSNLCPDSNFF
Sbjct: 1321 EFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADVRREAVAPTSLRIALSNLCPDSNFF 1380

Query: 1381 QEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASATCLVHSIFGMDI 1440
            QEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCAS TCLVHSIFGMDI
Sbjct: 1381 QEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASDTCLVHSIFGMDI 1440

Query: 1441 FERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQLACDLD 1500
            FERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQLACDLD
Sbjct: 1441 FERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQLACDLD 1500

Query: 1501 VGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYRGLDPK 1560
            VGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYRGLDPK
Sbjct: 1501 VGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYRGLDPK 1560

Query: 1561 HTHNLVSVVCYYGQHYHCFAYSHDKKCWIMYDDRTVKVIGGWPDVLTMCEKGHLQPQVLF 1620
            +THNLVSVVCYYGQHYHCFAYS+DKKCWIMYDDRTVKVIG W DVLTMCE+GHLQPQVLF
Sbjct: 1561 NTHNLVSVVCYYGQHYHCFAYSNDKKCWIMYDDRTVKVIGDWADVLTMCERGHLQPQVLF 1620

Query: 1621 FEAVN 1623
            FEAVN
Sbjct: 1621 FEAVN 1622

BLAST of Spg021901 vs. ExPASy TrEMBL
Match: A0A6J1C064 (uncharacterized protein LOC111007268 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007268 PE=4 SV=1)

HSP 1 Score: 2890.5 bits (7492), Expect = 0.0e+00
Identity = 1482/1631 (90.86%), Postives = 1540/1631 (94.42%), Query Frame = 0

Query: 1    MGRKKKVIDPRSKQSPAARGVAVDVPSLANAVDHSSIPDKASNHNQSRIEPVFVNESDGL 60
            MGRKKK++DPRSKQSPAARGVAVDVPS+AN+ D SS+PDK+SNHNQ+RIE VF NESDGL
Sbjct: 1    MGRKKKIVDPRSKQSPAARGVAVDVPSVANS-DQSSVPDKSSNHNQNRIESVFGNESDGL 60

Query: 61   SYSSIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120
            SYS+IKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID
Sbjct: 61   SYSTIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120

Query: 121  DLNTKQRHLKNAIESARKAVELSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180
            DLNTKQRHLKNAIESARKAVELSP+SIEFSHFYANLLYEVANDAKEYEEVVQECERALGI
Sbjct: 121  DLNTKQRHLKNAIESARKAVELSPSSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180

Query: 181  ENPIDPAKESLQDEKNQKIPTAEARITHVQTELRQLIQKSNIYSLSSWMKNLGNGEEKFR 240
            ENPIDPAKESLQDEKNQKIPTAEARI HVQTELRQLIQKSNIYS+SSWMKNLGNGEEKFR
Sbjct: 181  ENPIDPAKESLQDEKNQKIPTAEARIAHVQTELRQLIQKSNIYSISSWMKNLGNGEEKFR 240

Query: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSETPQMQN 300
            LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKS+TPQMQN
Sbjct: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSDTPQMQN 300

Query: 301  EGGKADRALDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSLESKKDLLKIK 360
            EGGKADR LDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMS+ESKKD LKIK
Sbjct: 301  EGGKADRTLDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSMESKKDFLKIK 360

Query: 361  TSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCRCDEKFVDSESHMHHVVQEHM 420
             SDL TH SSSKDASANEVISEALSF DANKTWKFW CCRCDEKFVDSESHMHHVVQEHM
Sbjct: 361  ISDLNTHISSSKDASANEVISEALSFCDANKTWKFWVCCRCDEKFVDSESHMHHVVQEHM 420

Query: 421  GNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKMLTDQRKFKDSELDEDICSQHH 480
            GNLLPKMQSILPQNVDNEW EMLLNCSWKALDVSAAAKMLTDQ K KDSEL EDIC QHH
Sbjct: 421  GNLLPKMQSILPQNVDNEWAEMLLNCSWKALDVSAAAKMLTDQTKCKDSELVEDICLQHH 480

Query: 481  SESDDCFKDAWDSSPVKHRYGNSLNECNLYEKVSNNGFKTCNENQGSLVSYSLSNSFPVS 540
            S+SDDCFKDAWDSSP KH YGN LNECN+YEKV NNGFK C+ENQGS+ SYSLS+SFPVS
Sbjct: 481  SDSDDCFKDAWDSSPEKHNYGNGLNECNVYEKVGNNGFKACHENQGSVTSYSLSDSFPVS 540

Query: 541  DDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDELQGLASGSHLLNQGLDQTPL 600
            DDSERTKLLEKIHA+FELLIRHKYLAASHLNKVIQFTMDELQGL SG HLLNQGLDQTPL
Sbjct: 541  DDSERTKLLEKIHAIFELLIRHKYLAASHLNKVIQFTMDELQGL-SGYHLLNQGLDQTPL 600

Query: 601  CICFLGASQLRKILKFLQELCQSCGIGRYSDKSTGQIEDSKSDKQPVEVKEKVVLNRDAS 660
            CICFLGASQLRKI KFLQEL  SCG GRY DKSTGQIEDSK+DKQ VEVKEK+VLN DAS
Sbjct: 601  CICFLGASQLRKIFKFLQELSHSCGFGRYVDKSTGQIEDSKNDKQSVEVKEKIVLNEDAS 660

Query: 661  LLILNECFLSSDIPHPNGQVAATDHMPA-TAKDVIANGVLSEVDPFLSWIYGGPSSGEQL 720
            LL+LNECFLSSD  H NGQ AATDH+P  TAKDV ANGV S+ DPFLSWIY G SSGEQL
Sbjct: 661  LLLLNECFLSSDFTHANGQAAATDHIPVPTAKDVTANGVFSDADPFLSWIYAGLSSGEQL 720

Query: 721  ASWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE 780
            +SWAQ+KEEKIQ+ MEI QMLEKEF HLQSLCERKCEHL+YEEALQ +EDLCLEEGKKRE
Sbjct: 721  SSWAQTKEEKIQQGMEILQMLEKEFLHLQSLCERKCEHLSYEEALQLLEDLCLEEGKKRE 780

Query: 781  TVTEFIPKSYESVLRRRRDELIEAENDVMFIGSRFELDAVTNVLKEAEALNVNQFGYEEN 840
             VTEFIPKSYESVLR+RR+ELI+ ENDVMF+ SRFELDA+TNVLKEAEALNVNQ+GY E 
Sbjct: 781  AVTEFIPKSYESVLRKRREELIDVENDVMFL-SRFELDAITNVLKEAEALNVNQYGYGET 840

Query: 841  FASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900
            F+S+P  LCDLESGEDEDWR KDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG
Sbjct: 841  FSSMPSQLCDLESGEDEDWRTKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900

Query: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK 960
            MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK
Sbjct: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK 960

Query: 961  KGIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLHDEAVDGDPFQVSSDGD 1020
            KG+RGG+DN RHAHEKPKEKKR+KEFRK KDSKVVSGPEQD LHDE VDGDPFQV+SDGD
Sbjct: 961  KGMRGGSDNLRHAHEKPKEKKRNKEFRKAKDSKVVSGPEQDALHDEDVDGDPFQVASDGD 1020

Query: 1021 AAEVDIAVFENGDALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080
            AAEVDIAVFENG+ALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK
Sbjct: 1021 AAEVDIAVFENGNALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080

Query: 1081 SAQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKPSVVDQLAENDLVADLSRNGPAMQGA- 1140
            SAQTN EK +AVA+HDNL+GLVP+VEGADE+ K SVVDQLAENDLV DLSRNGPAM  A 
Sbjct: 1081 SAQTNPEKKVAVAIHDNLLGLVPDVEGADEHFKLSVVDQLAENDLVPDLSRNGPAMSHAP 1140

Query: 1141 -----FSGISNVGVLENSLPSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNVTFDNQLN 1200
                  SG+SNVGVLEN L SSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDN  FD++LN
Sbjct: 1141 SGDQKISGVSNVGVLENCLSSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNAAFDSRLN 1200

Query: 1201 EQVRYHDNLLVDSVNPLSEDNSTKTLRR-HVEDDEERFQADLKKAVRQSLDAFQEQQKLP 1260
            E VRYH N L DSVNPLSEDNS KTLR+ HVEDDEERFQADLKKAVRQSLD FQEQQKLP
Sbjct: 1201 ETVRYHSNFLADSVNPLSEDNSKKTLRQHHVEDDEERFQADLKKAVRQSLDTFQEQQKLP 1260

Query: 1261 LITNSRTPLISYGEVDSNGLPSNELNVENV-GADIFGTGLKNEVGEYNCFLNVIIQSLWH 1320
            LI++SR PLIS G+VDSNG+PSNELNVENV GAD+FGTGLKNEVGEYNCFLNVIIQSLWH
Sbjct: 1261 LISSSRIPLISCGKVDSNGVPSNELNVENVQGADMFGTGLKNEVGEYNCFLNVIIQSLWH 1320

Query: 1321 LRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADARREAVAPTSLRIALSNLC 1380
            LRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASAD RREAVAPTSLRIALSNLC
Sbjct: 1321 LRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADVRREAVAPTSLRIALSNLC 1380

Query: 1381 PDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASATCLVHS 1440
            PDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCAS TCLVHS
Sbjct: 1381 PDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASDTCLVHS 1440

Query: 1441 IFGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQ 1500
            IFGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQ
Sbjct: 1441 IFGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQ 1500

Query: 1501 LACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLY 1560
            LACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLY
Sbjct: 1501 LACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLY 1560

Query: 1561 RGLDPKHTHNLVSVVCYYGQHYHCFAYSHDKKCWIMYDDRTVKVIGGWPDVLTMCEKGHL 1620
            RGLDPK+THNLVSVVCYYGQHYHCFAYS+DKKCWIMYDDRTVKVIG W DVLTMCE+GHL
Sbjct: 1561 RGLDPKNTHNLVSVVCYYGQHYHCFAYSNDKKCWIMYDDRTVKVIGDWADVLTMCERGHL 1620

Query: 1621 QPQVLFFEAVN 1623
            QPQVLFFEAVN
Sbjct: 1621 QPQVLFFEAVN 1628

BLAST of Spg021901 vs. ExPASy TrEMBL
Match: A0A6J1C4C5 (uncharacterized protein LOC111007268 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111007268 PE=4 SV=1)

HSP 1 Score: 2822.0 bits (7314), Expect = 0.0e+00
Identity = 1456/1630 (89.33%), Postives = 1512/1630 (92.76%), Query Frame = 0

Query: 1    MGRKKKVIDPRSKQSPAARGVAVDVPSLANAVDHSSIPDKASNHNQSRIEPVFVNESDGL 60
            MGRKKK++DPRSKQSPAARGVAVDVPS+AN+ D SS+PDK+SNHNQ+RIE VF NESDGL
Sbjct: 1    MGRKKKIVDPRSKQSPAARGVAVDVPSVANS-DQSSVPDKSSNHNQNRIESVFGNESDGL 60

Query: 61   SYSSIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120
            SYS+IKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID
Sbjct: 61   SYSTIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120

Query: 121  DLNTKQRHLKNAIESARKAVELSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180
            DLNTKQRHLKNAIESARKAVELSP+SIEFSHFYANLLYEVANDAKEYEEVVQECERALGI
Sbjct: 121  DLNTKQRHLKNAIESARKAVELSPSSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180

Query: 181  ENPIDPAKESLQDEKNQKIPTAEARITHVQTELRQLIQKSNIYSLSSWMKNLGNGEEKFR 240
            ENPIDPAKESLQDEKNQKIPTAEARI HVQTELRQLIQKSNIYS+SSWMKNLGNGEEKFR
Sbjct: 181  ENPIDPAKESLQDEKNQKIPTAEARIAHVQTELRQLIQKSNIYSISSWMKNLGNGEEKFR 240

Query: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSETPQMQN 300
            LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKS+TPQMQN
Sbjct: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSDTPQMQN 300

Query: 301  EGGKADRALDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSLESKKDLLKIK 360
            EGGKADR LDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMS+ESKKD LKIK
Sbjct: 301  EGGKADRTLDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSMESKKDFLKIK 360

Query: 361  TSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCRCDEKFVDSESHMHHVVQEHM 420
             SDL TH SSSKDASANEVISEALSF DANKTWKFW CCRCDEKFVDSESHMHHVVQEHM
Sbjct: 361  ISDLNTHISSSKDASANEVISEALSFCDANKTWKFWVCCRCDEKFVDSESHMHHVVQEHM 420

Query: 421  GNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKMLTDQRKFKDSELDEDICSQHH 480
            GNLLPKMQSILPQNVDNEW EMLLNCSWKALDVSAAAKMLTDQ K KDSEL EDIC QHH
Sbjct: 421  GNLLPKMQSILPQNVDNEWAEMLLNCSWKALDVSAAAKMLTDQTKCKDSELVEDICLQHH 480

Query: 481  SESDDCFKDAWDSSPVKHRYGNSLNECNLYEKVSNNGFKTCNENQGSLVSYSLSNSFPVS 540
            S+SDDCFKDAWDSSP KH YGN LNECN+YEKV NNGFK C+ENQGS+ SYSLS+SFPVS
Sbjct: 481  SDSDDCFKDAWDSSPEKHNYGNGLNECNVYEKVGNNGFKACHENQGSVTSYSLSDSFPVS 540

Query: 541  DDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDELQGLASGSHLLNQGLDQTPL 600
            DDSERTKLLEKIHA+FELLIRHKYLAASHLNKVIQFTMDELQGL SG HLLNQGLDQTPL
Sbjct: 541  DDSERTKLLEKIHAIFELLIRHKYLAASHLNKVIQFTMDELQGL-SGYHLLNQGLDQTPL 600

Query: 601  CICFLGASQLRKILKFLQELCQSCGIGRYSDKSTGQIEDSKSDKQPVEVKEKVVLNRDAS 660
            CICFLGASQLRKI KFLQEL  SCG GRY DKSTGQIEDSK+DKQ VEVKEK+VLN DAS
Sbjct: 601  CICFLGASQLRKIFKFLQELSHSCGFGRYVDKSTGQIEDSKNDKQSVEVKEKIVLNEDAS 660

Query: 661  LLILNECFLSSDIPHPNGQVAATDHMPATAKDVIANGVLSEVDPFLSWIYGGPSSGEQLA 720
            LL+LNECFLSSD  H NG                                   SSGEQL+
Sbjct: 661  LLLLNECFLSSDFTHANGL----------------------------------SSGEQLS 720

Query: 721  SWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRET 780
            SWAQ+KEEKIQ+ MEI QMLEKEF HLQSLCERKCEHL+YEEALQ +EDLCLEEGKKRE 
Sbjct: 721  SWAQTKEEKIQQGMEILQMLEKEFLHLQSLCERKCEHLSYEEALQLLEDLCLEEGKKREA 780

Query: 781  VTEFIPKSYESVLRRRRDELIEAENDVMFIGSRFELDAVTNVLKEAEALNVNQFGYEENF 840
            VTEFIPKSYESVLR+RR+ELI+ ENDVMF+ SRFELDA+TNVLKEAEALNVNQ+GY E F
Sbjct: 781  VTEFIPKSYESVLRKRREELIDVENDVMFL-SRFELDAITNVLKEAEALNVNQYGYGETF 840

Query: 841  ASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTGM 900
            +S+P  LCDLESGEDEDWR KDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTGM
Sbjct: 841  SSMPSQLCDLESGEDEDWRTKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTGM 900

Query: 901  QQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSKK 960
            QQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSKK
Sbjct: 901  QQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSKK 960

Query: 961  GIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLHDEAVDGDPFQVSSDGDA 1020
            G+RGG+DN RHAHEKPKEKKR+KEFRK KDSKVVSGPEQD LHDE VDGDPFQV+SDGDA
Sbjct: 961  GMRGGSDNLRHAHEKPKEKKRNKEFRKAKDSKVVSGPEQDALHDEDVDGDPFQVASDGDA 1020

Query: 1021 AEVDIAVFENGDALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKKS 1080
            AEVDIAVFENG+ALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKKS
Sbjct: 1021 AEVDIAVFENGNALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKKS 1080

Query: 1081 AQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKPSVVDQLAENDLVADLSRNGPAMQGA-- 1140
            AQTN EK +AVA+HDNL+GLVP+VEGADE+ K SVVDQLAENDLV DLSRNGPAM  A  
Sbjct: 1081 AQTNPEKKVAVAIHDNLLGLVPDVEGADEHFKLSVVDQLAENDLVPDLSRNGPAMSHAPS 1140

Query: 1141 ----FSGISNVGVLENSLPSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNVTFDNQLNE 1200
                 SG+SNVGVLEN L SSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDN  FD++LNE
Sbjct: 1141 GDQKISGVSNVGVLENCLSSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNAAFDSRLNE 1200

Query: 1201 QVRYHDNLLVDSVNPLSEDNSTKTLRR-HVEDDEERFQADLKKAVRQSLDAFQEQQKLPL 1260
             VRYH N L DSVNPLSEDNS KTLR+ HVEDDEERFQADLKKAVRQSLD FQEQQKLPL
Sbjct: 1201 TVRYHSNFLADSVNPLSEDNSKKTLRQHHVEDDEERFQADLKKAVRQSLDTFQEQQKLPL 1260

Query: 1261 ITNSRTPLISYGEVDSNGLPSNELNVENV-GADIFGTGLKNEVGEYNCFLNVIIQSLWHL 1320
            I++SR PLIS G+VDSNG+PSNELNVENV GAD+FGTGLKNEVGEYNCFLNVIIQSLWHL
Sbjct: 1261 ISSSRIPLISCGKVDSNGVPSNELNVENVQGADMFGTGLKNEVGEYNCFLNVIIQSLWHL 1320

Query: 1321 RRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADARREAVAPTSLRIALSNLCP 1380
            RRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASAD RREAVAPTSLRIALSNLCP
Sbjct: 1321 RRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADVRREAVAPTSLRIALSNLCP 1380

Query: 1381 DSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASATCLVHSI 1440
            DSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCAS TCLVHSI
Sbjct: 1381 DSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASDTCLVHSI 1440

Query: 1441 FGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQL 1500
            FGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQL
Sbjct: 1441 FGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQL 1500

Query: 1501 ACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYR 1560
            ACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYR
Sbjct: 1501 ACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYR 1560

Query: 1561 GLDPKHTHNLVSVVCYYGQHYHCFAYSHDKKCWIMYDDRTVKVIGGWPDVLTMCEKGHLQ 1620
            GLDPK+THNLVSVVCYYGQHYHCFAYS+DKKCWIMYDDRTVKVIG W DVLTMCE+GHLQ
Sbjct: 1561 GLDPKNTHNLVSVVCYYGQHYHCFAYSNDKKCWIMYDDRTVKVIGDWADVLTMCERGHLQ 1593

Query: 1621 PQVLFFEAVN 1623
            PQVLFFEAVN
Sbjct: 1621 PQVLFFEAVN 1593

BLAST of Spg021901 vs. ExPASy TrEMBL
Match: A0A6J1I805 (uncharacterized protein LOC111470828 OS=Cucurbita maxima OX=3661 GN=LOC111470828 PE=4 SV=1)

HSP 1 Score: 2805.8 bits (7272), Expect = 0.0e+00
Identity = 1444/1624 (88.92%), Postives = 1513/1624 (93.17%), Query Frame = 0

Query: 1    MGRKKKVIDPRSKQSPAARGVAVDVPSLANAVDHSSIPDKASNHNQSRIEPVFVNESDGL 60
            MG KKK+IDPRSKQSPAARGV VDVPS ANA D +SIP+KASNHNQSRIEPV +N  DGL
Sbjct: 1    MGPKKKIIDPRSKQSPAARGVVVDVPSSANAADQNSIPEKASNHNQSRIEPVLLNGPDGL 60

Query: 61   SYSSIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120
            SYS+IKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID
Sbjct: 61   SYSTIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120

Query: 121  DLNTKQRHLKNAIESARKAVELSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180
            DLNTKQRHLKNAIESARKAV+LSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI
Sbjct: 121  DLNTKQRHLKNAIESARKAVQLSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180

Query: 181  ENPIDPAKESLQDEKNQKIPTAEARITHVQTELRQLIQKSNIYSLSSWMKNLGNGEEKFR 240
            ENPIDPAKESLQDEK+QKIPTAEARITHVQTELRQLIQKSNIYS+SSWMKNLGNGEEKFR
Sbjct: 181  ENPIDPAKESLQDEKSQKIPTAEARITHVQTELRQLIQKSNIYSISSWMKNLGNGEEKFR 240

Query: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSETPQMQN 300
            LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQK ETPQ+QN
Sbjct: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKCETPQIQN 300

Query: 301  EGGKADRALDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSLESKKDLLKIK 360
            E GK DR+LDSSSGSGTRVGERRKHGNLRK GSSAERRD VHSFWNSMS+E+KKDL KIK
Sbjct: 301  EEGKVDRSLDSSSGSGTRVGERRKHGNLRKQGSSAERRDLVHSFWNSMSIEAKKDLFKIK 360

Query: 361  TSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCRCDEKFVDSESHMHHVVQEHM 420
             S+LK HFSSSKDASANEVISEALSFYDANKTWKFW CCRCDEKFVDS+SHMHHVVQEHM
Sbjct: 361  ISNLKAHFSSSKDASANEVISEALSFYDANKTWKFWVCCRCDEKFVDSDSHMHHVVQEHM 420

Query: 421  GNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKMLTDQRKFKDSELDEDICSQHH 480
            GNLLPKMQSILPQNVD+EWT+M+LNCSWKALDVSAAAKMLT + K KDSEL  DI  QHH
Sbjct: 421  GNLLPKMQSILPQNVDDEWTKMILNCSWKALDVSAAAKMLTTETKCKDSELVVDIFPQHH 480

Query: 481  SESDDCFKDAWDSSPVKHRYGNSLNECNLYEKVSNNGFKTCNENQGSLVSYSLSNSFPVS 540
            SE D+ FKDAWDSSP KH+YG+SLNEC L EK++NNG  T +ENQGS+ SYS S+SFPVS
Sbjct: 481  SECDNYFKDAWDSSPEKHKYGDSLNECRLSEKITNNGSMTYHENQGSVASYSSSDSFPVS 540

Query: 541  DDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDELQGLASGSHLLNQGLDQTPL 600
            DD ERTKLLEKIHAVFELLIRHKYLAASHLNKVIQF +DELQGLASGSHLLNQGLDQTPL
Sbjct: 541  DDPERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFAIDELQGLASGSHLLNQGLDQTPL 600

Query: 601  CICFLGASQLRKILKFLQELCQSCGIGRYSDKSTGQIEDSKSDKQPVEVKEKVVLNRDAS 660
            CICFLGASQLRKILKFLQEL QSCGIGRYSDK TGQIEDSKSDKQ VEV+EKVVL+ D S
Sbjct: 601  CICFLGASQLRKILKFLQELSQSCGIGRYSDKITGQIEDSKSDKQAVEVREKVVLSEDGS 660

Query: 661  LLILNECFLSSDIPHPNGQVAATDHM-PATAKDVIANGVLSEVDPFLSWIYGGPSSGEQL 720
            LL+LN+C LSSDI H +  VAA  HM  ATAKD+ +NGVLS+VDPFLSWIY GPSSGEQL
Sbjct: 661  LLLLNKCLLSSDITHADVPVAAAYHMLAATAKDLTSNGVLSDVDPFLSWIYAGPSSGEQL 720

Query: 721  ASWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE 780
             SW ++KEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE
Sbjct: 721  VSWEKTKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE 780

Query: 781  TVTEFIPKSYESVLRRRRDELIEAENDVMFIGSRFELDAVTNVLKEAEALNVNQFGYEEN 840
             + EFIPKSYESVLR+RR+ELIEAEN V+ I SRFELDAVTNVLKEAE LNVNQFGY+E 
Sbjct: 781  ALNEFIPKSYESVLRKRREELIEAENGVLLISSRFELDAVTNVLKEAETLNVNQFGYQET 840

Query: 841  FASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900
            FASV P L DLESGEDE+ RAKDYLH+VDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG
Sbjct: 841  FASVSPQLGDLESGEDEERRAKDYLHEVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900

Query: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK 960
            MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAA EAFLAELALDSK
Sbjct: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAASEAFLAELALDSK 960

Query: 961  KGIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLHDEAVDGDPFQVSSDGD 1020
            KGIRGG DNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVL  +AVDGDPF VSSDGD
Sbjct: 961  KGIRGGTDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLRGQAVDGDPFPVSSDGD 1020

Query: 1021 AAEVDIAVFENGDALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080
            AAEVDIAVFEN DALRLQEEE RRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK
Sbjct: 1021 AAEVDIAVFENVDALRLQEEELRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080

Query: 1081 SAQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKPSVVDQLAENDLVADLSRNGPAMQGAF 1140
            SAQT          H+NL GLVPNVEGAD++ KPSVVD+LAENDLV+DLS NGPAM+ A 
Sbjct: 1081 SAQT---------THNNLAGLVPNVEGADKHFKPSVVDKLAENDLVSDLSGNGPAMRNAL 1140

Query: 1141 SGISNVGVLENSLPSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNVTFDNQLNEQVRYH 1200
            SG+SNVGV+ENSLPSSDR+KGRKGRRHKVSIKPVDGNQPSPSDKDNV FDNQL EQVRYH
Sbjct: 1141 SGVSNVGVIENSLPSSDRKKGRKGRRHKVSIKPVDGNQPSPSDKDNVAFDNQLTEQVRYH 1200

Query: 1201 DNLLVDSVNPLSEDNSTKTLRRHVEDDEERFQADLKKAVRQSLDAFQEQQKLPLITNSRT 1260
             N L DSVNPL EDNSTKT R+HVED+EERFQADLKKAVRQSLDAFQEQQKLPLI +SRT
Sbjct: 1201 GNPLQDSVNPLLEDNSTKTSRQHVEDEEERFQADLKKAVRQSLDAFQEQQKLPLIMSSRT 1260

Query: 1261 PLISYGEVDSNGLPSNELNVENV-GADIFGTGLKNEVGEYNCFLNVIIQSLWHLRRFREE 1320
            PLIS+GEVDSNGLPSNE NVENV  A +FGTGLKNEVGEYNCFLNVIIQSLWHLRRFREE
Sbjct: 1261 PLISHGEVDSNGLPSNERNVENVQAAGMFGTGLKNEVGEYNCFLNVIIQSLWHLRRFREE 1320

Query: 1321 FLRRSMTEHVHVGDPCVVCALYEIFAALSMASADARREAVAPTSLRIALSNLCPDSNFFQ 1380
            FLRRSMTEHVHVGDPCVVCALYEIFAAL+ ASAD+RREAVAPTSLR+ALSNLCPDSNFFQ
Sbjct: 1321 FLRRSMTEHVHVGDPCVVCALYEIFAALNKASADSRREAVAPTSLRVALSNLCPDSNFFQ 1380

Query: 1381 EGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASATCLVHSIFGMDIF 1440
            EGQMNDASEVLAVIFDCLHQSLT  SSVSDT++VESNC+GSWDC+S TCLVHSIFGMDIF
Sbjct: 1381 EGQMNDASEVLAVIFDCLHQSLTAGSSVSDTDTVESNCIGSWDCSSDTCLVHSIFGMDIF 1440

Query: 1441 ERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQLACDLDV 1500
            ERMNCYSCGLESRHLKYTSFFHNINASALRT KVMC+ESSFDELLNVVEMNHQL CD DV
Sbjct: 1441 ERMNCYSCGLESRHLKYTSFFHNINASALRTTKVMCSESSFDELLNVVEMNHQLVCDQDV 1500

Query: 1501 GGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYRGLDPKH 1560
            GGCGKLNYIHHFLSAPPHVFTTVLGWQ TCESADDIT TLAALSTEID+SVLYRGLDPK+
Sbjct: 1501 GGCGKLNYIHHFLSAPPHVFTTVLGWQKTCESADDITETLAALSTEIDVSVLYRGLDPKN 1560

Query: 1561 THNLVSVVCYYGQHYHCFAYSHDKKCWIMYDDRTVKVIGGWPDVLTMCEKGHLQPQVLFF 1620
             HNLVSVVCYYGQHYHCFAYS DKKCWIMYDDRTVKVIGGW DVLTMCEKGHLQPQVLFF
Sbjct: 1561 VHNLVSVVCYYGQHYHCFAYSCDKKCWIMYDDRTVKVIGGWADVLTMCEKGHLQPQVLFF 1615

Query: 1621 EAVN 1623
            EAVN
Sbjct: 1621 EAVN 1615

BLAST of Spg021901 vs. ExPASy TrEMBL
Match: A0A6J1ENT1 (uncharacterized protein LOC111436182 OS=Cucurbita moschata OX=3662 GN=LOC111436182 PE=4 SV=1)

HSP 1 Score: 2805.0 bits (7270), Expect = 0.0e+00
Identity = 1447/1624 (89.10%), Postives = 1512/1624 (93.10%), Query Frame = 0

Query: 1    MGRKKKVIDPRSKQSPAARGVAVDVPSLANAVDHSSIPDKASNHNQSRIEPVFVNESDGL 60
            MG KKK+IDPRSKQSPAARGV VDVPS ANA D +SIP+KASNHNQSRIEPV +N  DGL
Sbjct: 1    MGPKKKIIDPRSKQSPAARGVVVDVPSSANAADQNSIPEKASNHNQSRIEPVLLNGPDGL 60

Query: 61   SYSSIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120
            SYS+IKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID
Sbjct: 61   SYSTIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120

Query: 121  DLNTKQRHLKNAIESARKAVELSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180
            DLNTKQRHLKNAIESARKAV+LSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI
Sbjct: 121  DLNTKQRHLKNAIESARKAVQLSPNSIEFSHFYANLLYEVANDAKEYEEVVQECERALGI 180

Query: 181  ENPIDPAKESLQDEKNQKIPTAEARITHVQTELRQLIQKSNIYSLSSWMKNLGNGEEKFR 240
            ENPIDPAKESLQDEK+QKIPTAEARITHVQTELRQLIQKSNIYS+SSWMKNLGNGEEKFR
Sbjct: 181  ENPIDPAKESLQDEKSQKIPTAEARITHVQTELRQLIQKSNIYSISSWMKNLGNGEEKFR 240

Query: 241  LIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSETPQMQN 300
            LIPIRRVTEDPMEVRLVQARR NEIKKATKTPEERRKEIEVRVAAARLLQQK ETPQ+QN
Sbjct: 241  LIPIRRVTEDPMEVRLVQARRTNEIKKATKTPEERRKEIEVRVAAARLLQQKCETPQIQN 300

Query: 301  EGGKADRALDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSLESKKDLLKIK 360
            E GK DR+LDSS GSGTR GERRKHGNLRK GSSAERRD VHSFWNSMS+E+KKDLLKIK
Sbjct: 301  EEGKVDRSLDSSLGSGTRAGERRKHGNLRKQGSSAERRDLVHSFWNSMSIEAKKDLLKIK 360

Query: 361  TSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCRCDEKFVDSESHMHHVVQEHM 420
             S+LK HFSSSKDASANEVISEALSFY+ANKTWKFW CCRCDEKFVDS+SHMHHVVQEHM
Sbjct: 361  ISNLKAHFSSSKDASANEVISEALSFYNANKTWKFWVCCRCDEKFVDSDSHMHHVVQEHM 420

Query: 421  GNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKMLTDQRKFKDSELDEDICSQHH 480
            GNLLPKMQSILPQNVD+EWT+M+LNCSWKALDVSAAAKMLT + K KDSEL   I  QHH
Sbjct: 421  GNLLPKMQSILPQNVDDEWTKMILNCSWKALDVSAAAKMLTAETKCKDSELVVGIFPQHH 480

Query: 481  SESDDCFKDAWDSSPVKHRYGNSLNECNLYEKVSNNGFKTCNENQGSLVSYSLSNSFPVS 540
            SE D+ FKDAW SSP KH+Y +SLNEC L EK++NNG KT  ENQGS+ SYS S+SFPVS
Sbjct: 481  SECDNYFKDAWGSSPEKHKYVDSLNECRLSEKITNNGSKTYLENQGSVASYSSSDSFPVS 540

Query: 541  DDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDELQGLASGSHLLNQGLDQTPL 600
            DD ERTKLLEKIHAVFELLIRHKYLAASHLNKVIQF MDELQGLASGSHLLNQGLDQTPL
Sbjct: 541  DDPERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFAMDELQGLASGSHLLNQGLDQTPL 600

Query: 601  CICFLGASQLRKILKFLQELCQSCGIGRYSDKSTGQIEDSKSDKQPVEVKEKVVLNRDAS 660
            CICFLGASQLRKILKFLQEL QSCGIGRYSDKSTGQIEDSKSDKQ VEV+EKVVL+ DAS
Sbjct: 601  CICFLGASQLRKILKFLQELSQSCGIGRYSDKSTGQIEDSKSDKQAVEVREKVVLSEDAS 660

Query: 661  LLILNECFLSSDIPHPNGQVAATDHM-PATAKDVIANGVLSEVDPFLSWIYGGPSSGEQL 720
            LL+LNEC LSSDI H +  VAA DHM  ATAKDV +NGVLS VDPFLSWIY GPSSGEQL
Sbjct: 661  LLLLNECLLSSDITHADVPVAAADHMLAATAKDVTSNGVLSGVDPFLSWIYAGPSSGEQL 720

Query: 721  ASWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE 780
             SW ++KEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE
Sbjct: 721  VSWEKTKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKKRE 780

Query: 781  TVTEFIPKSYESVLRRRRDELIEAENDVMFIGSRFELDAVTNVLKEAEALNVNQFGYEEN 840
             + EFIPKSYESVLR+RR+ELIEAEN V+FI SRFELDAVTNVLKEAE LNVNQFGY+E 
Sbjct: 781  ALNEFIPKSYESVLRKRREELIEAENGVLFISSRFELDAVTNVLKEAETLNVNQFGYQET 840

Query: 841  FASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900
            FASV P L DLESGEDE+ RAKDYLH+VDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG
Sbjct: 841  FASVSPQLGDLESGEDEERRAKDYLHEVDTCIEVAIQRQKEQLSIEISKIDARIMRNVTG 900

Query: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALDSK 960
            MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAA EAFLAELALDSK
Sbjct: 901  MQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAASEAFLAELALDSK 960

Query: 961  KGIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLHDEAVDGDPFQVSSDGD 1020
            KGIRGG DNSRHAHEKPKEKK+SKEFRKVKDSKVVSGPEQDV   +AVDGDPF VSSDGD
Sbjct: 961  KGIRGGTDNSRHAHEKPKEKKKSKEFRKVKDSKVVSGPEQDVHRGQAVDGDPFPVSSDGD 1020

Query: 1021 AAEVDIAVFENGDALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080
            AAEVDIAVFEN DALRLQEEE RRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK
Sbjct: 1021 AAEVDIAVFENVDALRLQEEELRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQKK 1080

Query: 1081 SAQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKPSVVDQLAENDLVADLSRNGPAMQGAF 1140
            SAQT          H+NL GLVPNVEGAD++ KPSVVD+LAENDLV+DLSRNGPAM+ A 
Sbjct: 1081 SAQT---------THNNLAGLVPNVEGADKHFKPSVVDKLAENDLVSDLSRNGPAMRNAL 1140

Query: 1141 SGISNVGVLENSLPSSDRRKGRKGRRHKVSIKPVDGNQPSPSDKDNVTFDNQLNEQVRYH 1200
            SG+SNVGVLENSLPSSDR+KGRKGRRHKVSIKPVDGNQPSPSDKDNV FDNQL EQVRYH
Sbjct: 1141 SGVSNVGVLENSLPSSDRKKGRKGRRHKVSIKPVDGNQPSPSDKDNVAFDNQLTEQVRYH 1200

Query: 1201 DNLLVDSVNPLSEDNSTKTLRRHVEDDEERFQADLKKAVRQSLDAFQEQQKLPLITNSRT 1260
             N L DSVNPL EDNSTKT R+HVED+EERFQADLKKAVRQSLDAFQEQQKLPLI +SRT
Sbjct: 1201 GNPLQDSVNPLLEDNSTKTSRQHVEDEEERFQADLKKAVRQSLDAFQEQQKLPLIMSSRT 1260

Query: 1261 PLISYGEVDSNGLPSNELNVENV-GADIFGTGLKNEVGEYNCFLNVIIQSLWHLRRFREE 1320
            PLIS+GEVDSNGLPSNE NVENV  A +FGTGLKNEVGEYNCFLNVIIQSLWHLRRFREE
Sbjct: 1261 PLISHGEVDSNGLPSNERNVENVQAAGMFGTGLKNEVGEYNCFLNVIIQSLWHLRRFREE 1320

Query: 1321 FLRRSMTEHVHVGDPCVVCALYEIFAALSMASADARREAVAPTSLRIALSNLCPDSNFFQ 1380
            FLRRSMTEHVHVGDPCVVCALYEIFAAL+ ASAD+RREAVAPTSLR+ALSNLCPDSNFFQ
Sbjct: 1321 FLRRSMTEHVHVGDPCVVCALYEIFAALNKASADSRREAVAPTSLRVALSNLCPDSNFFQ 1380

Query: 1381 EGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESNCMGSWDCASATCLVHSIFGMDIF 1440
            EGQMNDASEVLAVIFDCLHQSLT SSSVSDT+++ESNC+GSWDC+S TCLVHSIFGMDIF
Sbjct: 1381 EGQMNDASEVLAVIFDCLHQSLTASSSVSDTDTIESNCIGSWDCSSDTCLVHSIFGMDIF 1440

Query: 1441 ERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCTESSFDELLNVVEMNHQLACDLDV 1500
            ERMNCYSCGLESRHLKYTSFFHNINASALRT KVMC+ESSFDELLNVVEMNHQL CD DV
Sbjct: 1441 ERMNCYSCGLESRHLKYTSFFHNINASALRTTKVMCSESSFDELLNVVEMNHQLVCDQDV 1500

Query: 1501 GGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDITATLAALSTEIDISVLYRGLDPKH 1560
            GGCGKLNYIHHFLSAPPHVFTTVLGWQ TCESADDIT TLAALSTEIDISVLYRGLDPK+
Sbjct: 1501 GGCGKLNYIHHFLSAPPHVFTTVLGWQKTCESADDITETLAALSTEIDISVLYRGLDPKN 1560

Query: 1561 THNLVSVVCYYGQHYHCFAYSHDKKCWIMYDDRTVKVIGGWPDVLTMCEKGHLQPQVLFF 1620
             HNLVSVVCYYGQHYHCFAYS DKKCWIMYDDRTVKVIGGW DVLTMCEKGHLQPQVLFF
Sbjct: 1561 VHNLVSVVCYYGQHYHCFAYSCDKKCWIMYDDRTVKVIGGWADVLTMCEKGHLQPQVLFF 1615

Query: 1621 EAVN 1623
            EAVN
Sbjct: 1621 EAVN 1615

BLAST of Spg021901 vs. TAIR 10
Match: AT3G47910.1 (Ubiquitin carboxyl-terminal hydrolase-related protein )

HSP 1 Score: 1730.3 bits (4480), Expect = 0.0e+00
Identity = 954/1644 (58.03%), Postives = 1198/1644 (72.87%), Query Frame = 0

Query: 1    MGRKKKVIDPRSKQSPAARGVAVDVPSLANAVDHSSIPDKASNHNQSRIEPVFVNESDGL 60
            MG+KKK    RSKQ P++  V V V    + +   S  D   +   +  EP        L
Sbjct: 1    MGQKKKTSASRSKQPPSSTAVTV-VEVEPSEISACSFNDGGFSGPDANGEP--------L 60

Query: 61   SYSSIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120
              S +KLECE+AL +  RG++ KA+RL+K+  SR+++   SALIHRVQGTICVKVA++ +
Sbjct: 61   DSSVVKLECEKALKSFGRGSYNKAIRLIKDSCSRHQD---SALIHRVQGTICVKVAAVYE 120

Query: 121  DLNTKQRHLKNAIESARKAVELSPNSIEFSHFYANLLYEVANDAK-EYEEVVQECERALG 180
            DL TKQ++L+NAIESARKAVELSPNSIEF HFYANLLYE AN+ K EYEEVVQEC RAL 
Sbjct: 121  DLATKQKYLRNAIESARKAVELSPNSIEFGHFYANLLYEAANEGKREYEEVVQECHRALS 180

Query: 181  IENPIDPAKESLQDEKNQKIPTAEARITHVQTELRQLIQKSNIYSLSSWMKNLGNGEEKF 240
            IE PIDPA+ESLQDE   KI T EARI H Q ELR LIQKSNIYSLS+WM+ LGNGEEKF
Sbjct: 181  IEYPIDPARESLQDETQLKILTPEARIAHFQDELRSLIQKSNIYSLSTWMQTLGNGEEKF 240

Query: 241  RLIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSETPQMQ 300
            RLIP+RR+ EDP+E  L+Q+RR NEIKK+TKT EERRKEIEVRVAA RLLQQKSE+   +
Sbjct: 241  RLIPLRRMAEDPIESNLIQSRRPNEIKKSTKTNEERRKEIEVRVAAHRLLQQKSESSPSE 300

Query: 301  NEGGKADRALDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSLESKKDLLKI 360
            N     ++  DS+  +G R GERRKHGN RK+GS+A+RRD V S+W+S+S E KK+ L++
Sbjct: 301  NVEAVNNKGSDSALAAGPRSGERRKHGNARKNGSTADRRDRVRSYWDSLSKEMKKEFLRV 360

Query: 361  KTSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCRCDEKFVDSESHMHHVVQEH 420
            K SDLK+HFS SKD  A E++SEALSF +ANKTW+FWACCRC + F++SE+HMHH+VQ H
Sbjct: 361  KFSDLKSHFSGSKDGQAYEIVSEALSFCEANKTWRFWACCRCSQNFIESEAHMHHIVQAH 420

Query: 421  MGNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKMLTDQRKFKDSELDEDICSQH 480
            MGN+LPKMQ +LPQ VD E  +ML    WK LD+SA  K+L  Q+K ++SE DE     +
Sbjct: 421  MGNVLPKMQMVLPQIVDTERIDMLFKSPWKPLDLSATLKLLRSQQKIQNSEFDEFHSGDN 480

Query: 481  HSESDDCFKDAWDSSPVKHRYGNSLNECNLYEKVSNNGFKTCNENQGSLVSYSLSNSFPV 540
                DDCFKDAW+ +       N  NE    E+V  +            +++   + +P+
Sbjct: 481  MDGGDDCFKDAWNDTTPDGDTCNGWNENESEEEVKLS------------IAFPPPDGWPI 540

Query: 541  SDDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDELQGLASGSHLLNQGLDQTP 600
            SDD ER KLLEKI A FE LIRHKYLAASH +KVIQFT+DELQ L S S  LN+GL+++P
Sbjct: 541  SDDPERAKLLEKIRAAFEQLIRHKYLAASHHDKVIQFTLDELQNLPSVSQFLNRGLNKSP 600

Query: 601  LCICFLGASQLRKILKFLQELCQSCGIGRYSDKSTGQIEDSKSDKQPVEVKEKVVLNRDA 660
            LCICFLGAS L KILKFLQ+L Q+CG+ RYS++S    E +  D    EV E+++L+ + 
Sbjct: 601  LCICFLGASHLSKILKFLQDLSQACGLSRYSEQSNPHDEINFGDLGR-EVTEEILLDSED 660

Query: 661  SLLILNECFLSSDIPHPNGQVAATDHMPATAKDVIANG--VLSEVDPFLSWIYGGPSSGE 720
            S L+++E  L ++        +A +++   +   IANG  V S  D FLSWI+ GPSS E
Sbjct: 661  SCLLMDEKLLGTECIQEKYMGSAFNNVTIASSGDIANGNDVSSGADGFLSWIFTGPSSEE 720

Query: 721  QLASWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKK 780
            Q+ SW ++KE+K  + +EI Q LEKEFYHLQ+LCERKCEHL+YE ALQ+VEDLCLEEG+K
Sbjct: 721  QVVSWMRTKEDKTNQGLEIMQHLEKEFYHLQNLCERKCEHLSYEGALQTVEDLCLEEGRK 780

Query: 781  RETVTEFIPKSYESVLRRRRDELIEAENDVMFIGSRFELDAVTNVLKEAEALNVNQFGYE 840
            RET  EF  +SYE+VLR+RR+EL E++++++FI SRFELDA+TNVLK+AE LN NQFGYE
Sbjct: 781  RETSAEFTHESYETVLRKRREELNESDHELLFISSRFELDAITNVLKDAETLNHNQFGYE 840

Query: 841  ENFASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNV 900
            E++      L DLESGE ++W  KD LH+ D+ IE+AIQ+QKEQLS E+S+IDA++MRNV
Sbjct: 841  ESYGCTSSQLRDLESGEADEWGIKDSLHEADSFIEIAIQKQKEQLSAELSRIDAQMMRNV 900

Query: 901  TGMQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALD 960
            TGMQQLELKL PVS++DYQ +LLPLV SY+RAHLE LAEKDAT+KSDAAREA L ELALD
Sbjct: 901  TGMQQLELKLGPVSSNDYQIVLLPLVKSYMRAHLEALAEKDATEKSDAAREALLVELALD 960

Query: 961  SKKGIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLHDEAVDGDPFQVSSD 1020
            SKK  RG NDNS++  EK K+KK+ K+ RKVKD K   G +    + ++V+  P  V+  
Sbjct: 961  SKKEARGRNDNSKNTLEKSKDKKKIKDTRKVKDMKATIGSDHR-SNADSVEHSPLPVAPV 1020

Query: 1021 GDAAEVDIAVFENGDALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQ 1080
            GD +E DI V E  +AL+ +EEE++R+IELE +ERKLE+TLEYQRRIE+EAK+KH+AE Q
Sbjct: 1021 GDHSEADI-VSEAVEALKEEEEEYKRQIELEEEERKLEKTLEYQRRIEDEAKEKHMAEQQ 1080

Query: 1081 KK---SAQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKPSVVDQLAENDLVADLSRNGPA 1140
            KK   S   N+ K +     DN +  +  ++G ++ +     +   ++   A ++ NG  
Sbjct: 1081 KKYSSSVPMNVAKTVYNGCTDNEVDYLV-LQGQEKSINQEKRNGRLDDLEGASVNTNGVF 1140

Query: 1141 MQGAFSGISNVGVLEN--------------SLPSSDRRKGRKGRRHKVSIKPVDGNQP-S 1200
                 S IS+   ++N               +  SD+R GR+ RR K S K  DG  P +
Sbjct: 1141 PSTNHSAISDTAKVQNVKSQKVPNGTAMQAGIFQSDQRTGRRTRRQKASNKLADGKYPVT 1200

Query: 1201 PSDKDNVTFDNQLNEQVRYHDNLLVDSVNPLSEDNSTKTLR--RHVEDDEERFQADLKKA 1260
            P + +N           R+ + L  +       D  TKTLR  +  +DDEERFQAD+++A
Sbjct: 1201 PPETENSKSQLSGTNGERHSETLRNNG------DVGTKTLRQLQAEDDDEERFQADMQRA 1260

Query: 1261 VRQSLDAFQEQQKLPLITNSRTPLISYGEVDSNGLPSNEL--NVENVGADIFGTGLKNEV 1320
            V QSLD +Q  +   + +  RTPL    EV+++G  S++   +  + G  IFGTGL+NEV
Sbjct: 1261 VLQSLDVYQGGRN--MASCLRTPL----EVNNDGGLSDDTMESRSSTGVTIFGTGLQNEV 1320

Query: 1321 GEYNCFLNVIIQSLWHLRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADARR 1380
            GEYNCFLNVIIQSLW+L  FR EFLR S  EH HVGDPCVVC+LYEIF ALS AS++ + 
Sbjct: 1321 GEYNCFLNVIIQSLWNLELFRAEFLRSSTREHHHVGDPCVVCSLYEIFTALSAASSEKQN 1380

Query: 1381 EAVAPTSLRIALSNLCPDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVESN 1440
            E VAP+SLRIALSNL PDS+FFQE QMNDASEVLAVIFDCLH+S   SSSVSD +S +SN
Sbjct: 1381 EPVAPSSLRIALSNLYPDSSFFQEAQMNDASEVLAVIFDCLHRSFAQSSSVSDVDSSDSN 1440

Query: 1441 CMGSWDCASATCLVHSIFGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMCT 1500
            C GSWDCA+ TC+ HS+FGMDIFE++NCYSCGLESRHLKYTSFFHNINASALRTMKV C 
Sbjct: 1441 CTGSWDCANRTCIAHSLFGMDIFEQLNCYSCGLESRHLKYTSFFHNINASALRTMKVTCA 1500

Query: 1501 ESSFDELLNVVEMNHQLACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDIT 1560
            E+SFDELLN+VEMNHQLACD + GGCGKLN+IHH L+ PPHVFTTVLGWQNTCE+ +DI 
Sbjct: 1501 ENSFDELLNLVEMNHQLACDPETGGCGKLNHIHHILTTPPHVFTTVLGWQNTCETVEDIA 1560

Query: 1561 ATLAALSTEIDISVLYRGLDPKHTHNLVSVVCYYGQHYHCFAYSHDKKCWIMYDDRTVKV 1620
            ATLAAL+TEIDIS++YRGLDPK T++LVSVVCYYGQHYHCFA+S +   WIMYDD+TVKV
Sbjct: 1561 ATLAALNTEIDISIMYRGLDPKSTYSLVSVVCYYGQHYHCFAHSREHDQWIMYDDKTVKV 1604

BLAST of Spg021901 vs. TAIR 10
Match: AT3G47910.2 (Ubiquitin carboxyl-terminal hydrolase-related protein )

HSP 1 Score: 1729.9 bits (4479), Expect = 0.0e+00
Identity = 954/1645 (57.99%), Postives = 1198/1645 (72.83%), Query Frame = 0

Query: 1    MGRKKKVIDPRSKQSPAARGVAVDVPSLANAVDHSSIPDKASNHNQSRIEPVFVNESDGL 60
            MG+KKK    RSKQ P++  V V V    + +   S  D   +   +  EP        L
Sbjct: 1    MGQKKKTSASRSKQPPSSTAVTV-VEVEPSEISACSFNDGGFSGPDANGEP--------L 60

Query: 61   SYSSIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASIID 120
              S +KLECE+AL +  RG++ KA+RL+K+  SR+++   SALIHRVQGTICVKVA++ +
Sbjct: 61   DSSVVKLECEKALKSFGRGSYNKAIRLIKDSCSRHQD---SALIHRVQGTICVKVAAVYE 120

Query: 121  DLNTKQRHLKNAIESARKAVELSPNSIEFSHFYANLLYEVANDAK-EYEEVVQECERALG 180
            DL TKQ++L+NAIESARKAVELSPNSIEF HFYANLLYE AN+ K EYEEVVQEC RAL 
Sbjct: 121  DLATKQKYLRNAIESARKAVELSPNSIEFGHFYANLLYEAANEGKREYEEVVQECHRALS 180

Query: 181  IENPIDPAKESLQDEKNQKIPTAEARITHVQTELRQLIQKSNIYSLSSWMKNLGNGEEKF 240
            IE PIDPA+ESLQDE   KI T EARI H Q ELR LIQKSNIYSLS+WM+ LGNGEEKF
Sbjct: 181  IEYPIDPARESLQDETQLKILTPEARIAHFQDELRSLIQKSNIYSLSTWMQTLGNGEEKF 240

Query: 241  RLIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSETPQMQ 300
            RLIP+RR+ EDP+E  L+Q+RR NEIKK+TKT EERRKEIEVRVAA RLLQQKSE+   +
Sbjct: 241  RLIPLRRMAEDPIESNLIQSRRPNEIKKSTKTNEERRKEIEVRVAAHRLLQQKSESSPSE 300

Query: 301  NEGGKADRALDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSLESKKDLLKI 360
            N     ++  DS+  +G R GERRKHGN RK+GS+A+RRD V S+W+S+S E KK+ L++
Sbjct: 301  NVEAVNNKGSDSALAAGPRSGERRKHGNARKNGSTADRRDRVRSYWDSLSKEMKKEFLRV 360

Query: 361  KTSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCRCDEKFVDSESHMHHVVQEH 420
            K SDLK+HFS SKD  A E++SEALSF +ANKTW+FWACCRC + F++SE+HMHH+VQ H
Sbjct: 361  KFSDLKSHFSGSKDGQAYEIVSEALSFCEANKTWRFWACCRCSQNFIESEAHMHHIVQAH 420

Query: 421  MGNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKMLTDQRKFKDSELDEDICSQH 480
            MGN+LPKMQ +LPQ VD E  +ML    WK LD+SA  K+L  Q+K ++SE DE     +
Sbjct: 421  MGNVLPKMQMVLPQIVDTERIDMLFKSPWKPLDLSATLKLLRSQQKIQNSEFDEFHSGDN 480

Query: 481  HSESDDCFKDAWDSSPVKHRYGNSLNECNLYEKVSNNGFKTCNENQGSLVSYSLSNSFPV 540
                DDCFKDAW+ +       N  NE    E+V  +            +++   + +P+
Sbjct: 481  MDGGDDCFKDAWNDTTPDGDTCNGWNENESEEEVKLS------------IAFPPPDGWPI 540

Query: 541  SDDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDELQGLASGSHLLNQGLDQTP 600
            SDD ER KLLEKI A FE LIRHKYLAASH +KVIQFT+DELQ L S S  LN+GL+++P
Sbjct: 541  SDDPERAKLLEKIRAAFEQLIRHKYLAASHHDKVIQFTLDELQNLPSVSQFLNRGLNKSP 600

Query: 601  LCICFLGASQLRKILKFLQELCQSCGIGRYSDKSTGQIEDSKSDKQPVEVKEKVVLNRDA 660
            LCICFLGAS L KILKFLQ+L Q+CG+ RYS++S    E +  D    EV E+++L+ + 
Sbjct: 601  LCICFLGASHLSKILKFLQDLSQACGLSRYSEQSNPHDEINFGDLGR-EVTEEILLDSED 660

Query: 661  SLLILNECFLSSDIPHPNGQVAATDHMPATAKDVIANG--VLSEVDPFLSWIYGGPSSGE 720
            S L+++E  L ++        +A +++   +   IANG  V S  D FLSWI+ GPSS E
Sbjct: 661  SCLLMDEKLLGTECIQEKYMGSAFNNVTIASSGDIANGNDVSSGADGFLSWIFTGPSSEE 720

Query: 721  QLASWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLCLEEGKK 780
            Q+ SW ++KE+K  + +EI Q LEKEFYHLQ+LCERKCEHL+YE ALQ+VEDLCLEEG+K
Sbjct: 721  QVVSWMRTKEDKTNQGLEIMQHLEKEFYHLQNLCERKCEHLSYEGALQTVEDLCLEEGRK 780

Query: 781  RETVTEFIPKSYESVLRRRRDELIEAENDVMFIGSRFELDAVTNVLKEAEALNVNQFGYE 840
            RET  EF  +SYE+VLR+RR+EL E++++++FI SRFELDA+TNVLK+AE LN NQFGYE
Sbjct: 781  RETSAEFTHESYETVLRKRREELNESDHELLFISSRFELDAITNVLKDAETLNHNQFGYE 840

Query: 841  ENFASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEVAIQRQKEQLSIEISKIDARIMRNV 900
            E++      L DLESGE ++W  KD LH+ D+ IE+AIQ+QKEQLS E+S+IDA++MRNV
Sbjct: 841  ESYGCTSSQLRDLESGEADEWGIKDSLHEADSFIEIAIQKQKEQLSAELSRIDAQMMRNV 900

Query: 901  TGMQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFLAELALD 960
            TGMQQLELKL PVS++DYQ +LLPLV SY+RAHLE LAEKDAT+KSDAAREA L ELALD
Sbjct: 901  TGMQQLELKLGPVSSNDYQIVLLPLVKSYMRAHLEALAEKDATEKSDAAREALLVELALD 960

Query: 961  SKKGIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLHDEAVDGDPFQVSSD 1020
            SKK  RG NDNS++  EK K+KK+ K+ RKVKD K   G +    + ++V+  P  V+  
Sbjct: 961  SKKEARGRNDNSKNTLEKSKDKKKIKDTRKVKDMKATIGSDHR-SNADSVEHSPLPVAPV 1020

Query: 1021 GDAAEVDIAVFENGDALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQKHLAELQ 1080
            GD +E DI V E  +AL+ +EEE++R+IELE +ERKLE+TLEYQRRIE+EAK+KH+AE Q
Sbjct: 1021 GDHSEADI-VSEAVEALKEEEEEYKRQIELEEEERKLEKTLEYQRRIEDEAKEKHMAEQQ 1080

Query: 1081 KK---SAQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKPSVVDQLAENDLVADLSRNGPA 1140
            KK   S   N+ K +     DN +  +  ++G ++ +     +   ++   A ++ NG  
Sbjct: 1081 KKYSSSVPMNVAKTVYNGCTDNEVDYLV-LQGQEKSINQEKRNGRLDDLEGASVNTNGVF 1140

Query: 1141 MQGAFSGISNVGVLEN---------------SLPSSDRRKGRKGRRHKVSIKPVDGNQP- 1200
                 S IS+   ++N                +  SD+R GR+ RR K S K  DG  P 
Sbjct: 1141 PSTNHSAISDTAKVQNVKSQKVVPNGTAMQAGIFQSDQRTGRRTRRQKASNKLADGKYPV 1200

Query: 1201 SPSDKDNVTFDNQLNEQVRYHDNLLVDSVNPLSEDNSTKTLR--RHVEDDEERFQADLKK 1260
            +P + +N           R+ + L  +       D  TKTLR  +  +DDEERFQAD+++
Sbjct: 1201 TPPETENSKSQLSGTNGERHSETLRNNG------DVGTKTLRQLQAEDDDEERFQADMQR 1260

Query: 1261 AVRQSLDAFQEQQKLPLITNSRTPLISYGEVDSNGLPSNEL--NVENVGADIFGTGLKNE 1320
            AV QSLD +Q  +   + +  RTPL    EV+++G  S++   +  + G  IFGTGL+NE
Sbjct: 1261 AVLQSLDVYQGGRN--MASCLRTPL----EVNNDGGLSDDTMESRSSTGVTIFGTGLQNE 1320

Query: 1321 VGEYNCFLNVIIQSLWHLRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADAR 1380
            VGEYNCFLNVIIQSLW+L  FR EFLR S  EH HVGDPCVVC+LYEIF ALS AS++ +
Sbjct: 1321 VGEYNCFLNVIIQSLWNLELFRAEFLRSSTREHHHVGDPCVVCSLYEIFTALSAASSEKQ 1380

Query: 1381 REAVAPTSLRIALSNLCPDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVES 1440
             E VAP+SLRIALSNL PDS+FFQE QMNDASEVLAVIFDCLH+S   SSSVSD +S +S
Sbjct: 1381 NEPVAPSSLRIALSNLYPDSSFFQEAQMNDASEVLAVIFDCLHRSFAQSSSVSDVDSSDS 1440

Query: 1441 NCMGSWDCASATCLVHSIFGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVMC 1500
            NC GSWDCA+ TC+ HS+FGMDIFE++NCYSCGLESRHLKYTSFFHNINASALRTMKV C
Sbjct: 1441 NCTGSWDCANRTCIAHSLFGMDIFEQLNCYSCGLESRHLKYTSFFHNINASALRTMKVTC 1500

Query: 1501 TESSFDELLNVVEMNHQLACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADDI 1560
             E+SFDELLN+VEMNHQLACD + GGCGKLN+IHH L+ PPHVFTTVLGWQNTCE+ +DI
Sbjct: 1501 AENSFDELLNLVEMNHQLACDPETGGCGKLNHIHHILTTPPHVFTTVLGWQNTCETVEDI 1560

Query: 1561 TATLAALSTEIDISVLYRGLDPKHTHNLVSVVCYYGQHYHCFAYSHDKKCWIMYDDRTVK 1620
             ATLAAL+TEIDIS++YRGLDPK T++LVSVVCYYGQHYHCFA+S +   WIMYDD+TVK
Sbjct: 1561 AATLAALNTEIDISIMYRGLDPKSTYSLVSVVCYYGQHYHCFAHSREHDQWIMYDDKTVK 1605

BLAST of Spg021901 vs. TAIR 10
Match: AT3G47890.1 (Ubiquitin carboxyl-terminal hydrolase-related protein )

HSP 1 Score: 1692.9 bits (4383), Expect = 0.0e+00
Identity = 939/1594 (58.91%), Postives = 1164/1594 (73.02%), Query Frame = 0

Query: 60   LSYSSIKLECERALTALRRGNHTKALRLMKELSSRNENSVHSALIHRVQGTICVKVASII 119
            L  + IKLECE+AL +  RG++ KA+RL+K+  SR+++   SALIHRVQGTICVKVA++ 
Sbjct: 11   LDSALIKLECEKALKSFGRGSYNKAIRLIKDSCSRHQD---SALIHRVQGTICVKVAAVY 70

Query: 120  DDLNTKQRHLKNAIESARKAVELSPNSIEFSHFYANLLYEVANDAK-EYEEVVQECERAL 179
            +DL TKQ++L+NAIESARKAVELSP+SIEF HFYANLLYE AND K EY+EVVQEC RAL
Sbjct: 71   EDLATKQKYLRNAIESARKAVELSPDSIEFGHFYANLLYEAANDGKREYDEVVQECHRAL 130

Query: 180  GIENPIDPAKESLQDEKNQKIPTAEARITHVQTELRQLIQKSNIYSLSSWMKNLGNGEEK 239
             IENPIDPAKESLQDE   KI T EARI HVQ ELR LIQKSNI SLS+WM NLG GEEK
Sbjct: 131  SIENPIDPAKESLQDETQLKILTPEARIVHVQDELRSLIQKSNISSLSTWMNNLGKGEEK 190

Query: 240  FRLIPIRRVTEDPMEVRLVQARRANEIKKATKTPEERRKEIEVRVAAARLLQQKSETPQM 299
            FRLIPIRR+ EDP+E  LVQ RR NEIKKA K+ EE RKE+EVRVAAARLLQQKSE    
Sbjct: 191  FRLIPIRRMAEDPIESNLVQTRRPNEIKKANKSIEEIRKEVEVRVAAARLLQQKSE---C 250

Query: 300  QNEGGKADRALDSSSGSGTRVGERRKHGNLRKHGSSAERRDWVHSFWNSMSLESKKDLLK 359
            +N G   ++  D++ GSG R GERRKHGN R++GS+A+RRD V S+W+SMS E KK LL+
Sbjct: 251  ENVGAVDNKGSDATLGSGKRSGERRKHGNARRNGSTADRRDRVKSYWDSMSKEMKKQLLR 310

Query: 360  IKTSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCRCDEKFVDSESHMHHVVQE 419
            +K SDLK+HFS+SKD + NE+ISEALSF +ANKTW+FW CC+C EKF+ SE++MHH+VQ 
Sbjct: 311  VKLSDLKSHFSASKDGNENEIISEALSFCEANKTWRFWVCCQCSEKFIKSEAYMHHIVQV 370

Query: 420  HMGNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKMLTDQRKFKDSELDEDICSQ 479
            HMGN+LPKMQ +LPQ+ D E  +MLL   WK LD+SAA K L  ++K ++SE  E     
Sbjct: 371  HMGNVLPKMQMVLPQSFDTERIDMLLTSPWKPLDLSAAVKWLRSRQKIQNSEFSEFHAGD 430

Query: 480  HHSESDDCFKDAW-DSSPVKHRYGNSLNECNLYEKVSNNGFKTCNEN---QGSL-VSYSL 539
            +  + DDCFKDA  D+SP K   G+S               K C+EN   +G L +++  
Sbjct: 431  NMDDGDDCFKDARNDTSPEKESLGDSC--------------KGCDENDPEEGKLSITFPP 490

Query: 540  SNSFPVSDDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDELQGLASGSHLLNQ 599
             + +P+SDD ER KLLEKI A FELLIRHKYLAASH +KVIQFT+DELQ LAS S  LN+
Sbjct: 491  PDGWPISDDPERAKLLEKIRAAFELLIRHKYLAASHHDKVIQFTLDELQNLASVSQFLNR 550

Query: 600  GLDQTPLCICFLGASQLRKILKFLQELCQSCGIGRYSDKSTGQIEDSKSDKQPVEVKEKV 659
             L+Q+P+CICFLGASQLRKILKFLQ+L Q+CG+ RYS++S    ++        EV E++
Sbjct: 551  SLNQSPICICFLGASQLRKILKFLQDLSQACGLSRYSEQSNPN-DEFNFGALCREVTEEI 610

Query: 660  VLNRDASLLILNECFLSSDIPHPNGQVAATDHMPATAKDVIANG--VLSEVDPFLSWIYG 719
            +L+ + S L+L+E  L ++        +A  ++   +   IANG  V S  D FLSWI+ 
Sbjct: 611  LLDGEDSCLLLDEKLLGTECIQEIYMGSALHNVAVASSGDIANGNDVSSGADGFLSWIFT 670

Query: 720  GPSSGEQLASWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKCEHLNYEEALQSVEDLC 779
            GPSS EQ+ SW ++KEEK  + +EI Q LEKEFYHLQ+LCERKCEHL+YE ALQ+VEDLC
Sbjct: 671  GPSSEEQVVSWMRTKEEKTNQGLEIMQHLEKEFYHLQNLCERKCEHLSYEGALQTVEDLC 730

Query: 780  LEEGKKRETVTEFIPKSYESVLRRRRDELIEAENDVMFIGSRFELDAVTNVLKEAEALNV 839
            LEE +KRET  EF  +SYESVLR+RR+EL E + +++FI SRFELDA+TNVLK+AE LN 
Sbjct: 731  LEESRKRETSAEFTHESYESVLRKRREELNENDLELVFISSRFELDALTNVLKDAETLNH 790

Query: 840  NQFGYEENFASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEVAIQRQKEQLSIEISKIDA 899
            NQFGYEE++      L DLESGE ++W  KD LH+ D+ IEVAIQ+QKEQLS E+S+IDA
Sbjct: 791  NQFGYEESYGCTSSQLRDLESGEADEWGMKDSLHEADSFIEVAIQKQKEQLSAELSRIDA 850

Query: 900  RIMRNVTGMQQLELKLEPVSAHDYQSILLPLVNSYLRAHLEDLAEKDATKKSDAAREAFL 959
            ++MRNVTGMQQLELKL PVS++DYQ +LLPLV SY+RAHLE LAEKDAT+KSDAAREA L
Sbjct: 851  QMMRNVTGMQQLELKLGPVSSNDYQIVLLPLVKSYMRAHLEALAEKDATEKSDAAREALL 910

Query: 960  AELALDSKKGIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKVVSGPEQDVLHDEAVDGDP 1019
             ELALDSKK  RG NDNS+H  EK K+KK+ K+ RK+K+ K   G +    + ++++   
Sbjct: 911  FELALDSKKEGRGRNDNSKHTLEKSKDKKKIKDTRKLKNLKATIGNDHR-FNGDSIEHSL 970

Query: 1020 FQVSSDGDAAEVDIAVFENGDALRLQEEEFRRKIELEADERKLEETLEYQRRIENEAKQK 1079
              V+S GD +E D+ V E  +AL  +EEE+RR  ELE +ERKL +TLEYQRRIENEAK+K
Sbjct: 971  LSVASFGDHSEADV-VSEAIEALSDEEEEYRRCNELEEEERKLAKTLEYQRRIENEAKEK 1030

Query: 1080 HLAELQKKSAQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKPSVVDQLAENDLVADLSRN 1139
            H+AE QKK    +   N+  AV+D+ I    +     E  KP  ++Q   N  + DL   
Sbjct: 1031 HIAE-QKKKYSCSDPMNVTEAVYDDCIENFFDDADLQEQEKP--INQEKWNKQLDDLEGA 1090

Query: 1140 GPAMQGAFSG--------------------ISNVGVLENSLPSSDRRKGRKGRRHKVSIK 1199
               + G F                      + N   ++  +  SD+R GR+GRR K S K
Sbjct: 1091 KVNINGVFPSTNHCVISDTAKVLDVISQEVVPNGIAIQAGVFQSDQRPGRRGRRQKASNK 1150

Query: 1200 PVDGN-QPSPSDKDNVTFDNQLNEQVRYHDNLLVDSVNPLSEDNSTKTLR--RHVEDDEE 1259
             VDG  Q + S+ ++        +  R  + L  +       D   KTLR  +  +D+EE
Sbjct: 1151 LVDGKYQVTLSESEDSKSQRSGTDSERQSETLRSNG------DAGPKTLRQLQAEDDEEE 1210

Query: 1260 RFQADLKKAVRQSLDAFQEQQKLPLITNSRTPLISYGEVDSNGLPSNELNVE---NVGAD 1319
            RFQADLK+A  QSLD ++ ++ +       T  +     D+N    +++  E   + G  
Sbjct: 1211 RFQADLKRAKLQSLDVYRGRRNM-------TSCLRTSLEDNNDWVLSDVTKESQSSPGVA 1270

Query: 1320 IFGTGLKNEVGEYNCFLNVIIQSLWHLRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAA 1379
            IFGTGL+NEVGEYNCFLNVIIQSLW+L  FR EFLR S  EH H GDPCVVC+LY IF A
Sbjct: 1271 IFGTGLQNEVGEYNCFLNVIIQSLWNLGMFRAEFLRSSTLEHHHFGDPCVVCSLYAIFTA 1330

Query: 1380 LSMASADARREAVAPTSLRIALSNLCPDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSS 1439
            LS AS++ R+E VAP+SLRIALSNL PDS+FFQE QMNDASEVLAVIFDCLH+S   SSS
Sbjct: 1331 LSTASSETRKEPVAPSSLRIALSNLYPDSSFFQEAQMNDASEVLAVIFDCLHRSFAQSSS 1390

Query: 1440 VSDTESVESNCMGSWDCASATCLVHSIFGMDIFERMNCYSCGLESRHLKYTSFFHNINAS 1499
            VSDTES ESN  GSWDCA+ +C+ HS+FGMD+ E++NCYSCGLESRHLKYTSFFHNINAS
Sbjct: 1391 VSDTESAESNSTGSWDCANRSCIAHSLFGMDVSEQLNCYSCGLESRHLKYTSFFHNINAS 1450

Query: 1500 ALRTMKVMCTESSFDELLNVVEMNHQLACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQ 1559
            ALRTMKV C E+SFDELLN+VEMNHQLACD + GGCGK N+IHH L+ PPHVFT VLGWQ
Sbjct: 1451 ALRTMKVTCAENSFDELLNLVEMNHQLACDREAGGCGKRNHIHHILTTPPHVFTIVLGWQ 1510

Query: 1560 NTCESADDITATLAALSTEIDISVLYRGLDPKHTHNLVSVVCYYGQHYHCFAYSHDKKCW 1619
            NTCE+ +DI ATLAAL+TEIDIS++YRG+DPK+T++LVSVVCYYGQHYHCFAYSH+   W
Sbjct: 1511 NTCETVEDIAATLAALNTEIDISIMYRGVDPKNTYSLVSVVCYYGQHYHCFAYSHEHDQW 1565

BLAST of Spg021901 vs. TAIR 10
Match: AT1G65120.2 (Ubiquitin carboxyl-terminal hydrolase-related protein )

HSP 1 Score: 189.1 bits (479), Expect = 2.8e-47
Identity = 261/1289 (20.25%), Postives = 472/1289 (36.62%), Query Frame = 0

Query: 341  VHSFWNSMSLESKKDLLKIKTSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCR 400
            + S+W SM +ESK++ +K+  + L  +          + + + L     N+ WKFW C  
Sbjct: 213  LRSYWASMDVESKRNFMKVSIAKLTAYVERLYGRVGLDALEQVLDSARINRKWKFWMCRS 272

Query: 401  CDEKFVDSESHMHHVVQEHMGNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKML 460
            C +KF   +    H+ QEH     P     + Q VD  W  M+    W+ +DV AA +M+
Sbjct: 273  CSQKFYYPKKFRSHLEQEHAAKFKPSTTKHMAQRVDEVWAGMISVAGWEPVDVVAAVEMI 332

Query: 461  TDQRKFKDSELDEDICSQHHSESDDCFKDAWDSSPVKHRYGNSLNECNLYEKVSNNGFKT 520
              Q +                                          ++ E V  NG+  
Sbjct: 333  KTQLE------------------------------------------SVKEFVYENGW-- 392

Query: 521  CNENQGSLVSYSLSNSFPVSDDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDE 580
                         S ++P++ D ER+KLL+++ ++  L    K L+ S  + ++Q T+  
Sbjct: 393  -------------SKNWPLAVDEERSKLLKELQSLLLLFQERKILSCSIRDWMMQLTVKH 452

Query: 581  LQGLASGSHLLNQ--GLDQTPLCICFLGASQLRKILKFLQEL-CQSCGIGRYSDKSTGQI 640
            L  L    H L+    L +TP  ICFL   +L +IL  L+ + C+          +T   
Sbjct: 453  LAQLEISEHTLSSKCRLVETPQSICFLECHELNQILDLLKRINCERVDGTELVSNAT--- 512

Query: 641  EDSKSDKQPVEVKEKVVLNRDASLLILNECFLSSDIP--HPNGQVAATDHMPATAKDVIA 700
             DS   +  + VKEK+  + + S ++L++  L   I      G +   DH      +V  
Sbjct: 513  -DSLCGR--LRVKEKIEFDHEFSFMLLDKRLLRGKIASFDDEGIIDVCDH------NVHY 572

Query: 701  NGVLSEVDPFLSWIYGGPSSGEQLASWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKC 760
                 + D  ++W+   P   E        +   ++  + + + +      L +   +K 
Sbjct: 573  TKTHPQGDDTITWLLDYPLIDESFEFPRSIRAHNLEICVAVLRAIHFTCATLGTKYAKKL 632

Query: 761  EHLNYEEALQSVEDLCLEEGKKRETVTEFIPKSYESVLRRRRDE--LIEAENDVMFIGSR 820
            + LNY+ AL   ++LC+ E ++R+ V E    +Y S+L  + +E  +I+A +      ++
Sbjct: 633  QILNYDAALIDAKNLCILEDERRKNVPEDQWNTYASLLCDKCEERLVIDAGDSHT---TK 692

Query: 821  FELDAVTNVLKEAEALNVNQFGYEENFASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEV 880
              L AV +VL++A                   P  +    ED       + +  D  +  
Sbjct: 693  LCLCAVRDVLEKAS-----------------HPTFEFSDLEDCMTLIHGHKNLNDDTVLK 752

Query: 881  AIQRQKEQLSIEISKIDARIMRNVTGMQQLELKLEPVSAHDYQSILLPLVNSYLRAHLED 940
            +I   K  ++ ++   D++I+        L   L  +S  DY+S +L L+  Y R  L++
Sbjct: 753  SIDLLKSVVTNKVPVADSKILLIENSRTNLLNDLVKLSVFDYRSYILHLLKRYQRDELDE 812

Query: 941  LAEKDATKKSDAAREAFLAELALDSKKGIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKV 1000
            + + DA  K  AA+   L+E                    EK ++ K+S   +K      
Sbjct: 813  IVDMDAKTKLAAAQAEHLSE--------------------EKQEKGKKSGSNKK------ 872

Query: 1001 VSGPEQDVLHDEAVDGDPFQVSSDGDAAEVDIAVFENGDALRLQEEEFRRKIELEADERK 1060
                                                                        
Sbjct: 873  ------------------------------------------------------------ 932

Query: 1061 LEETLEYQRRIENEAKQKHLAELQKKSAQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKP 1120
                       +N + ++  A + K              VH++ + L P V         
Sbjct: 933  -----------KNRSNKRTSASMSKDD------------VHESSVNLEPKV--------- 992

Query: 1121 SVVDQLAENDLVADLSRNGPAMQGAFSGISNVGVLENSLPSSDRRKGRKGRRHKVSIKPV 1180
                                      +  S     E+S+   D     +GR         
Sbjct: 993  --------------------------TPPSPKSTEEDSMEPEDTLSSERGR--------- 1052

Query: 1181 DGNQPSPSDKDNVTFDNQLNEQVRYHDNLLVDSVNPLSEDNSTKTLRRHVEDDEERFQAD 1240
                                         L  S N  S+D + K                
Sbjct: 1053 -----------------------------LEISSNTKSQDETAK---------------- 1112

Query: 1241 LKKAVRQSLDAFQEQQKLPLITNSRTPLISYGEVDSNGLPSNELNVENVGADIFGTGLKN 1300
                                              D   +P  +L  EN+           
Sbjct: 1113 ----------------------------------DMENMPGEDLLSENL------ESAHK 1144

Query: 1301 EVGEYNCFLNVIIQSLWHLRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADA 1360
            +V  YN  L++ +++L +++  +E+ +      H ++ +  V CAL + F+A  + S   
Sbjct: 1173 KVTRYNSALDMTLKALLNIKVLQEDLVHNRQLFHGNLEEQ-VPCALKDFFSA--VVSEQI 1144

Query: 1361 RREAVAPTSLRIALSNLCPDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVE 1420
            + + +    L   L++L    +       +DA EV+  I + LH                
Sbjct: 1233 KEDELYSYLLSNLLASLEEVHSL-----SSDADEVVVTILEFLHW--------------- 1144

Query: 1421 SNCMGSWDCASATCLVHSIFGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVM 1480
                  W       LV  +F ++ +ERM C  C     + + +S+   + A+++R +K  
Sbjct: 1293 ------WKSPEKESLVTRLFTLEEYERMRCTKCRRNPNYPEQSSYGIVMAANSIRNLKCA 1144

Query: 1481 CTESSFDELLNVVEMNHQLACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADD 1540
                 F+E+L V+ M  ++ CD+  GGCGK N++HH LS  P +FT VL W+   E+  +
Sbjct: 1353 FGNMKFEEILTVIRMEDEMICDVKTGGCGKTNFVHHVLSRCPPIFTIVLEWEKN-ETETE 1144

Query: 1541 ITATLAALSTEIDISVLYRGL-DPKHTHNLVSVV-CYYGQHYHCFAYSHDKKCWIMYDDR 1600
            I+ T  AL+ EIDIS LY GL +P   + LVS++ C     + C AY  ++   + ++  
Sbjct: 1413 ISETAKALAWEIDISRLYEGLEEPNTNYRLVSMIGCGEEGEHICMAYKTNRWVSLRHEAL 1144

Query: 1601 TVKVIGGWPDVLTMCEKGHLQPQVLFFEA 1621
              + +G W +V+ +C +  ++P++LF+EA
Sbjct: 1473 LEEGVGNWKNVVRICGERRVRPEILFYEA 1144

BLAST of Spg021901 vs. TAIR 10
Match: AT1G65120.1 (Ubiquitin carboxyl-terminal hydrolase-related protein )

HSP 1 Score: 164.9 bits (416), Expect = 5.6e-40
Identity = 252/1247 (20.21%), Postives = 447/1247 (35.85%), Query Frame = 0

Query: 341  VHSFWNSMSLESKKDLLKIKTSDLKTHFSSSKDASANEVISEALSFYDANKTWKFWACCR 400
            + S+W SM +ESK++ +K+  + L  +          + + + L     N+ WKFW C  
Sbjct: 213  LRSYWASMDVESKRNFMKVSIAKLTAYVERLYGRVGLDALEQVLDSARINRKWKFWMCRS 272

Query: 401  CDEKFVDSESHMHHVVQEHMGNLLPKMQSILPQNVDNEWTEMLLNCSWKALDVSAAAKML 460
            C +KF   +    H+ QEH     P     + Q VD  W  M+    W+ +DV AA +M+
Sbjct: 273  CSQKFYYPKKFRSHLEQEHAAKFKPSTTKHMAQRVDEVWAGMISVAGWEPVDVVAAVEMI 332

Query: 461  TDQRKFKDSELDEDICSQHHSESDDCFKDAWDSSPVKHRYGNSLNECNLYEKVSNNGFKT 520
              Q +                                          ++ E V  NG+  
Sbjct: 333  KTQLE------------------------------------------SVKEFVYENGW-- 392

Query: 521  CNENQGSLVSYSLSNSFPVSDDSERTKLLEKIHAVFELLIRHKYLAASHLNKVIQFTMDE 580
                         S ++P++ D ER+KLL+++ ++  L    K L+ S  + ++Q T+  
Sbjct: 393  -------------SKNWPLAVDEERSKLLKELQSLLLLFQERKILSCSIRDWMMQLTVKH 452

Query: 581  LQGLASGSHLLNQ--GLDQTPLCICFLGASQLRKILKFLQEL-CQSCGIGRYSDKSTGQI 640
            L  L    H L+    L +TP  ICFL   +L +IL  L+ + C+          +T   
Sbjct: 453  LAQLEISEHTLSSKCRLVETPQSICFLECHELNQILDLLKRINCERVDGTELVSNAT--- 512

Query: 641  EDSKSDKQPVEVKEKVVLNRDASLLILNECFLSSDIP--HPNGQVAATDHMPATAKDVIA 700
             DS   +  + VKEK+  + + S ++L++  L   I      G +   DH      +V  
Sbjct: 513  -DSLCGR--LRVKEKIEFDHEFSFMLLDKRLLRGKIASFDDEGIIDVCDH------NVHY 572

Query: 701  NGVLSEVDPFLSWIYGGPSSGEQLASWAQSKEEKIQRRMEIFQMLEKEFYHLQSLCERKC 760
                 + D  ++W+   P   E        +   ++  + + + +      L +   +K 
Sbjct: 573  TKTHPQGDDTITWLLDYPLIDESFEFPRSIRAHNLEICVAVLRAIHFTCATLGTKYAKKL 632

Query: 761  EHLNYEEALQSVEDLCLEEGKKRETVTEFIPKSYESVLRRRRDE--LIEAENDVMFIGSR 820
            + LNY+ AL   ++LC+ E ++R+ V E    +Y S+L  + +E  +I+A +      ++
Sbjct: 633  QILNYDAALIDAKNLCILEDERRKNVPEDQWNTYASLLCDKCEERLVIDAGDSHT---TK 692

Query: 821  FELDAVTNVLKEAEALNVNQFGYEENFASVPPPLCDLESGEDEDWRAKDYLHQVDTCIEV 880
              L AV +VL++A                   P  +    ED       + +  D  +  
Sbjct: 693  LCLCAVRDVLEKAS-----------------HPTFEFSDLEDCMTLIHGHKNLNDDTVLK 752

Query: 881  AIQRQKEQLSIEISKIDARIMRNVTGMQQLELKLEPVSAHDYQSILLPLVNSYLRAHLED 940
            +I   K  ++ ++   D++I+        L   L  +S  DY+S +L L+  Y R  L++
Sbjct: 753  SIDLLKSVVTNKVPVADSKILLIENSRTNLLNDLVKLSVFDYRSYILHLLKRYQRDELDE 812

Query: 941  LAEKDATKKSDAAREAFLAELALDSKKGIRGGNDNSRHAHEKPKEKKRSKEFRKVKDSKV 1000
            + + DA  K  AA+   L+E                    EK ++ K+S   +K      
Sbjct: 813  IVDMDAKTKLAAAQAEHLSE--------------------EKQEKGKKSGSNKK------ 872

Query: 1001 VSGPEQDVLHDEAVDGDPFQVSSDGDAAEVDIAVFENGDALRLQEEEFRRKIELEADERK 1060
                                                                        
Sbjct: 873  ------------------------------------------------------------ 932

Query: 1061 LEETLEYQRRIENEAKQKHLAELQKKSAQTNLEKNLAVAVHDNLIGLVPNVEGADEYLKP 1120
                       +N + ++  A + K              VH++ + L P V         
Sbjct: 933  -----------KNRSNKRTSASMSKDD------------VHESSVNLEPKV--------- 992

Query: 1121 SVVDQLAENDLVADLSRNGPAMQGAFSGISNVGVLENSLPSSDRRKGRKGRRHKVSIKPV 1180
                                      +  S     E+S+   D     +GR         
Sbjct: 993  --------------------------TPPSPKSTEEDSMEPEDTLSSERGR--------- 1052

Query: 1181 DGNQPSPSDKDNVTFDNQLNEQVRYHDNLLVDSVNPLSEDNSTKTLRRHVEDDEERFQAD 1240
                                         L  S N  S+D + K                
Sbjct: 1053 -----------------------------LEISSNTKSQDETAK---------------- 1102

Query: 1241 LKKAVRQSLDAFQEQQKLPLITNSRTPLISYGEVDSNGLPSNELNVENVGADIFGTGLKN 1300
                                              D   +P  +L  EN+           
Sbjct: 1113 ----------------------------------DMENMPGEDLLSENL------ESAHK 1102

Query: 1301 EVGEYNCFLNVIIQSLWHLRRFREEFLRRSMTEHVHVGDPCVVCALYEIFAALSMASADA 1360
            +V  YN  L++ +++L +++  +E+ +      H ++ +  V CAL + F+A  + S   
Sbjct: 1173 KVTRYNSALDMTLKALLNIKVLQEDLVHNRQLFHGNLEEQ-VPCALKDFFSA--VVSEQI 1102

Query: 1361 RREAVAPTSLRIALSNLCPDSNFFQEGQMNDASEVLAVIFDCLHQSLTPSSSVSDTESVE 1420
            + + +    L   L++L    +       +DA EV+  I + LH                
Sbjct: 1233 KEDELYSYLLSNLLASLEEVHSL-----SSDADEVVVTILEFLHW--------------- 1102

Query: 1421 SNCMGSWDCASATCLVHSIFGMDIFERMNCYSCGLESRHLKYTSFFHNINASALRTMKVM 1480
                  W       LV  +F ++ +ERM C  C     + + +S+   + A+++R +K  
Sbjct: 1293 ------WKSPEKESLVTRLFTLEEYERMRCTKCRRNPNYPEQSSYGIVMAANSIRNLKCA 1102

Query: 1481 CTESSFDELLNVVEMNHQLACDLDVGGCGKLNYIHHFLSAPPHVFTTVLGWQNTCESADD 1540
                 F+E+L V+ M  ++ CD+  GGCGK N++HH LS  P +FT VL W+   E+  +
Sbjct: 1353 FGNMKFEEILTVIRMEDEMICDVKTGGCGKTNFVHHVLSRCPPIFTIVLEWEKN-ETETE 1102

Query: 1541 ITATLAALSTEIDISVLYRGL-DPKHTHNLVSVVCYYGQHYH-CFAY 1579
            I+ T  AL+ EIDIS LY GL +P   + LVS+V     H H C+ +
Sbjct: 1413 ISETAKALAWEIDISRLYEGLEEPNTNYRLVSMVYLKQGHKHFCYLF 1102

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135262.10.0e+0091.20uncharacterized protein LOC111007268 isoform X2 [Momordica charantia][more]
XP_022135261.10.0e+0090.86uncharacterized protein LOC111007268 isoform X1 [Momordica charantia][more]
XP_022135263.10.0e+0089.33uncharacterized protein LOC111007268 isoform X3 [Momordica charantia][more]
XP_022972240.10.0e+0088.92uncharacterized protein LOC111470828 [Cucurbita maxima][more]
KAG7021496.10.0e+0089.04Inactive ubiquitin carboxyl-terminal hydrolase 54, partial [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
Q8BL061.6e-3632.94Inactive ubiquitin carboxyl-terminal hydrolase 54 OS=Mus musculus OX=10090 GN=Us... [more]
Q70EK82.1e-3632.16Inactive ubiquitin carboxyl-terminal hydrolase 53 OS=Homo sapiens OX=9606 GN=USP... [more]
Q70EL14.8e-3632.65Inactive ubiquitin carboxyl-terminal hydrolase 54 OS=Homo sapiens OX=9606 GN=USP... [more]
Q6IE248.1e-3632.36Inactive ubiquitin carboxyl-terminal hydrolase 54 OS=Rattus norvegicus OX=10116 ... [more]
P159752.0e-3431.58Inactive ubiquitin carboxyl-terminal hydrolase 53 OS=Mus musculus OX=10090 GN=Us... [more]
Match NameE-valueIdentityDescription
A0A6J1C2740.0e+0091.20uncharacterized protein LOC111007268 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1C0640.0e+0090.86uncharacterized protein LOC111007268 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1C4C50.0e+0089.33uncharacterized protein LOC111007268 isoform X3 OS=Momordica charantia OX=3673 G... [more]
A0A6J1I8050.0e+0088.92uncharacterized protein LOC111470828 OS=Cucurbita maxima OX=3661 GN=LOC111470828... [more]
A0A6J1ENT10.0e+0089.10uncharacterized protein LOC111436182 OS=Cucurbita moschata OX=3662 GN=LOC1114361... [more]
Match NameE-valueIdentityDescription
AT3G47910.10.0e+0058.03Ubiquitin carboxyl-terminal hydrolase-related protein [more]
AT3G47910.20.0e+0057.99Ubiquitin carboxyl-terminal hydrolase-related protein [more]
AT3G47890.10.0e+0058.91Ubiquitin carboxyl-terminal hydrolase-related protein [more]
AT1G65120.22.8e-4720.25Ubiquitin carboxyl-terminal hydrolase-related protein [more]
AT1G65120.15.6e-4020.21Ubiquitin carboxyl-terminal hydrolase-related protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 119..139
NoneNo IPR availableCOILSCoilCoilcoord: 1047..1086
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 1277..1620
e-value: 4.7E-33
score: 116.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 961..996
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1146..1187
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 289..334
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 963..996
NoneNo IPR availablePANTHERPTHR22975UBIQUITIN SPECIFIC PROTEINASEcoord: 1..1622
NoneNo IPR availablePANTHERPTHR22975:SF28INACTIVE UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 53-LIKEcoord: 1..1622
NoneNo IPR availableCDDcd02257Peptidase_C19coord: 1381..1619
e-value: 8.39551E-16
score: 77.1382
IPR006866Domain of unknown function DUF627, N-terminalPFAMPF04781DUF627coord: 71..181
e-value: 3.2E-32
score: 110.8
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 61..205
e-value: 2.8E-8
score: 35.8
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 65..182
IPR006865Domain of unknown function DUF629PFAMPF04780DUF629coord: 341..873
e-value: 4.4E-158
score: 526.7
IPR001394Peptidase C19, ubiquitin carboxyl-terminal hydrolasePFAMPF00443UCHcoord: 1289..1597
e-value: 6.1E-23
score: 81.7
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 398..419
IPR028889Ubiquitin specific protease domainPROSITEPS50235USP_3coord: 1289..1621
score: 21.534861
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 1289..1619

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg021901.1Spg021901.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016579 protein deubiquitination
molecular_function GO:0005515 protein binding
molecular_function GO:0004843 thiol-dependent deubiquitinase