Spg021830 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg021830
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAmino acid transporter, putative
Locationscaffold2: 14448354 .. 14449802 (-)
RNA-Seq ExpressionSpg021830
SyntenySpg021830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGACCAAAAACAAGAACTCAAGTGTAACCACAACCACAACCAATCCACCGCCGATTCCTCTTCCATTTCTCAAGACCTTCCCACCACCACCACGGCCACCGCTCCGACCGCCGCCAGCAACAAGCTCACTCTAATCCCCCTCATCTTCATCATCTACTTCCAGGTCTCCGGCGGTCCCTATGACGAAGAGTCGGCCGTTCAGGCCGCCGGACCTCTCCTCACCATCATCGGCTTCCTCGTCTTCCCATTCATATGGAGTGTCCCGGAGGCGCTGATCACGGCGGAGCTCTCCACCACCTTCCCCGGCGACGGCGGATACGTGATATGGGCCGAGAGAGCCTTCGGACCCTTCTGGGGCTCTCTCATCGGAACCTGGAAACTCCTCAGCGGCATCATCAACATCACTGCCTGCCCCGTCCTCTGCGTTGAATACATGAAGAAAATCGCGACGCCACTCGAATCCGGCTGGCCCCGTCGCACCGCCATCCTCGCCTCCACCCTCCTCCTCTCCCTTCTCAACTACATTGGACTGACCATCGTCGGATACGTGGCCGTGGTCTTAGCATTCTTGTCCCTCTTACCGTTCATGATAATGACCTTCATGGCCATCCCCATGGTTCGGCCCCGTCGATGGCTCAGTTTGGGCGATAAAGGGGTCAAAAAGGATTGGAATCTCTATCTCAACACTATGTTTTGGAACCTCAATTACTGGGATAACGTCAGCACTCTCGCCGGAGAAGTCCAAAACCCCAAGAAAACATTCCCAATCGCTCTGTTCGTGTCAGTGATTTTCACTTGTCTTTCTTATTTAATCCCAATTCTGGCCGTGGTCGGCGCCGTTGATGTAGAGCAATCCACTTGGGAGTCTGGATTCCACGCCCAAGCCGCGGAGACCATCGCCGGCGGAAAATGGCTCAAAATCCTCCTCGAAATTGGAGAGTGCTTGTCGGCGATTGGGCTGTTCGAGGCCCAATTGAGCAGCAGCGCGTACCAGATTCTGGGCATGGCGGAGATTGGGATTCTGCCCAAGTTGTTGGCGGGCAGAGCAAAATGGTTCAACACTCCATGGATTGGAATTGTGATTTGCACTGCAATTTCGCTGGCGGTTTCGTATATGAACTTCGACGACATCGTTGCGACGGCGAATTTCATATACAGCTTGGGAATGCTGCTGGAGTTTTCGTCGTTCGTTTGGTTGAGATGGAAGCATCCGGGTATGAAAAGGCCGTTCAAGGTTCCATTGAAGTTGCCGGGTTTGATTGTGATGTGTTTGGTTTCGTCTGCGTTTTTTGTGGTGGTGATGTATTTTACTCACAAGAAAGTGTTCTTGGTGAGTGCTGCAATGACTGCGGCTGGGGTTATCTGGTTTGAGTTGATGAAGATTTGTAAGAAGAAAGAGATATTCGAGTTCAATCCTCGCCCTGAAGCCATTCAAGAATCATGA

mRNA sequence

ATGGCTGACCAAAAACAAGAACTCAAGTGTAACCACAACCACAACCAATCCACCGCCGATTCCTCTTCCATTTCTCAAGACCTTCCCACCACCACCACGGCCACCGCTCCGACCGCCGCCAGCAACAAGCTCACTCTAATCCCCCTCATCTTCATCATCTACTTCCAGGTCTCCGGCGGTCCCTATGACGAAGAGTCGGCCGTTCAGGCCGCCGGACCTCTCCTCACCATCATCGGCTTCCTCGTCTTCCCATTCATATGGAGTGTCCCGGAGGCGCTGATCACGGCGGAGCTCTCCACCACCTTCCCCGGCGACGGCGGATACGTGATATGGGCCGAGAGAGCCTTCGGACCCTTCTGGGGCTCTCTCATCGGAACCTGGAAACTCCTCAGCGGCATCATCAACATCACTGCCTGCCCCGTCCTCTGCGTTGAATACATGAAGAAAATCGCGACGCCACTCGAATCCGGCTGGCCCCGTCGCACCGCCATCCTCGCCTCCACCCTCCTCCTCTCCCTTCTCAACTACATTGGACTGACCATCGTCGGATACGTGGCCGTGGTCTTAGCATTCTTGTCCCTCTTACCGTTCATGATAATGACCTTCATGGCCATCCCCATGGTTCGGCCCCGTCGATGGCTCAGTTTGGGCGATAAAGGGGTCAAAAAGGATTGGAATCTCTATCTCAACACTATGTTTTGGAACCTCAATTACTGGGATAACGTCAGCACTCTCGCCGGAGAAGTCCAAAACCCCAAGAAAACATTCCCAATCGCTCTGTTCGTGTCAGTGATTTTCACTTGTCTTTCTTATTTAATCCCAATTCTGGCCGTGGTCGGCGCCGTTGATGTAGAGCAATCCACTTGGGAGTCTGGATTCCACGCCCAAGCCGCGGAGACCATCGCCGGCGGAAAATGGCTCAAAATCCTCCTCGAAATTGGAGAGTGCTTGTCGGCGATTGGGCTGTTCGAGGCCCAATTGAGCAGCAGCGCGTACCAGATTCTGGGCATGGCGGAGATTGGGATTCTGCCCAAGTTGTTGGCGGGCAGAGCAAAATGGTTCAACACTCCATGGATTGGAATTGTGATTTGCACTGCAATTTCGCTGGCGGTTTCGTATATGAACTTCGACGACATCGTTGCGACGGCGAATTTCATATACAGCTTGGGAATGCTGCTGGAGTTTTCGTCGTTCGTTTGGTTGAGATGGAAGCATCCGGGTATGAAAAGGCCGTTCAAGGTTCCATTGAAGTTGCCGGGTTTGATTGTGATGTGTTTGGTTTCGTCTGCGTTTTTTGTGGTGGTGATGTATTTTACTCACAAGAAAGTGTTCTTGGTGAGTGCTGCAATGACTGCGGCTGGGGTTATCTGGTTTGAGTTGATGAAGATTTGTAAGAAGAAAGAGATATTCGAGTTCAATCCTCGCCCTGAAGCCATTCAAGAATCATGA

Coding sequence (CDS)

ATGGCTGACCAAAAACAAGAACTCAAGTGTAACCACAACCACAACCAATCCACCGCCGATTCCTCTTCCATTTCTCAAGACCTTCCCACCACCACCACGGCCACCGCTCCGACCGCCGCCAGCAACAAGCTCACTCTAATCCCCCTCATCTTCATCATCTACTTCCAGGTCTCCGGCGGTCCCTATGACGAAGAGTCGGCCGTTCAGGCCGCCGGACCTCTCCTCACCATCATCGGCTTCCTCGTCTTCCCATTCATATGGAGTGTCCCGGAGGCGCTGATCACGGCGGAGCTCTCCACCACCTTCCCCGGCGACGGCGGATACGTGATATGGGCCGAGAGAGCCTTCGGACCCTTCTGGGGCTCTCTCATCGGAACCTGGAAACTCCTCAGCGGCATCATCAACATCACTGCCTGCCCCGTCCTCTGCGTTGAATACATGAAGAAAATCGCGACGCCACTCGAATCCGGCTGGCCCCGTCGCACCGCCATCCTCGCCTCCACCCTCCTCCTCTCCCTTCTCAACTACATTGGACTGACCATCGTCGGATACGTGGCCGTGGTCTTAGCATTCTTGTCCCTCTTACCGTTCATGATAATGACCTTCATGGCCATCCCCATGGTTCGGCCCCGTCGATGGCTCAGTTTGGGCGATAAAGGGGTCAAAAAGGATTGGAATCTCTATCTCAACACTATGTTTTGGAACCTCAATTACTGGGATAACGTCAGCACTCTCGCCGGAGAAGTCCAAAACCCCAAGAAAACATTCCCAATCGCTCTGTTCGTGTCAGTGATTTTCACTTGTCTTTCTTATTTAATCCCAATTCTGGCCGTGGTCGGCGCCGTTGATGTAGAGCAATCCACTTGGGAGTCTGGATTCCACGCCCAAGCCGCGGAGACCATCGCCGGCGGAAAATGGCTCAAAATCCTCCTCGAAATTGGAGAGTGCTTGTCGGCGATTGGGCTGTTCGAGGCCCAATTGAGCAGCAGCGCGTACCAGATTCTGGGCATGGCGGAGATTGGGATTCTGCCCAAGTTGTTGGCGGGCAGAGCAAAATGGTTCAACACTCCATGGATTGGAATTGTGATTTGCACTGCAATTTCGCTGGCGGTTTCGTATATGAACTTCGACGACATCGTTGCGACGGCGAATTTCATATACAGCTTGGGAATGCTGCTGGAGTTTTCGTCGTTCGTTTGGTTGAGATGGAAGCATCCGGGTATGAAAAGGCCGTTCAAGGTTCCATTGAAGTTGCCGGGTTTGATTGTGATGTGTTTGGTTTCGTCTGCGTTTTTTGTGGTGGTGATGTATTTTACTCACAAGAAAGTGTTCTTGGTGAGTGCTGCAATGACTGCGGCTGGGGTTATCTGGTTTGAGTTGATGAAGATTTGTAAGAAGAAAGAGATATTCGAGTTCAATCCTCGCCCTGAAGCCATTCAAGAATCATGA

Protein sequence

MADQKQELKCNHNHNQSTADSSSISQDLPTTTTATAPTAASNKLTLIPLIFIIYFQVSGGPYDEESAVQAAGPLLTIIGFLVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGPFWGSLIGTWKLLSGIINITACPVLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIGLTIVGYVAVVLAFLSLLPFMIMTFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYWDNVSTLAGEVQNPKKTFPIALFVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAAETIAGGKWLKILLEIGECLSAIGLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPWIGIVICTAISLAVSYMNFDDIVATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMCLVSSAFFVVVMYFTHKKVFLVSAAMTAAGVIWFELMKICKKKEIFEFNPRPEAIQES
Homology
BLAST of Spg021830 vs. NCBI nr
Match: XP_038878316.1 (probable polyamine transporter At3g13620 [Benincasa hispida])

HSP 1 Score: 771.9 bits (1992), Expect = 3.2e-219
Identity = 396/482 (82.16%), Postives = 434/482 (90.04%), Query Frame = 0

Query: 1   MADQKQELKCNHNHNQ-STADSSSISQDLPTTTTATAPTAASNKLTLIPLIFIIYFQVSG 60
           MAD+KQ+   N N NQ +TADSS  +Q LPTTTT  AP AA  KLTLIPLIF+IYF+V+G
Sbjct: 1   MADKKQQSLNNFNSNQPATADSS--TQILPTTTT-PAPAAARKKLTLIPLIFLIYFEVAG 60

Query: 61  GPYDEESAVQAAGPLLTIIGFLVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGPF 120
           GPY EE AVQAAGPLL IIGF+VFPFIWSVPEALITAELST FPG+GG+VIWAERAFGPF
Sbjct: 61  GPYGEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPF 120

Query: 121 WGSLIGTWKLLSGIINITACPVLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIGL 180
           WGSL+GTWKLLSG+INI A PVLC++Y+KK+A PLESGWPRR AILASTL+L+LLNYIGL
Sbjct: 121 WGSLMGTWKLLSGVINIAAFPVLCIDYIKKMAPPLESGWPRRIAILASTLILALLNYIGL 180

Query: 181 TIVGYVAVVLAFLSLLPFMIMTFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYW 240
           TIVGYVAVVLAFLSLLPF++MTF++IP ++P RWL LGDK  K+DWNLYLNT+FWNLN+W
Sbjct: 181 TIVGYVAVVLAFLSLLPFILMTFISIPKIKPHRWLILGDK--KRDWNLYLNTLFWNLNFW 240

Query: 241 DNVSTLAGEVQNPKKTFPIALFVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAAE 300
           DNVSTLAGEV+NP+KTFP+ALFVSVIFTCLSYLIP+LAV+GAVDVEQS W SGFHAQAAE
Sbjct: 241 DNVSTLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAE 300

Query: 301 TIAGGKWLKILLEIGECLSAIGLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPWI 360
           TIA GKWLKILLEIG  LSAIGLFEAQLSSSAYQILGMAEIGILPK  A RAKWFNTPWI
Sbjct: 301 TIA-GKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWI 360

Query: 361 GIVICTAISLAVSYMNFDDIVATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLP 420
           GIVICTAISLAVSYM+F DIVA+ANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLP
Sbjct: 361 GIVICTAISLAVSYMDFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLP 420

Query: 421 GLIVMCLVSSAFFVVVMYFTHKKVFLVSAAMTAAGVIWFELMKICKKKEIFEFNPRPEAI 480
           GLIVMCL+ SAF VVVM FTH  VFLVSAAMT  G++WF LMKICKKK+I EFNP  EAI
Sbjct: 421 GLIVMCLIPSAFLVVVMVFTHTNVFLVSAAMTVGGILWFGLMKICKKKKILEFNPETEAI 476

Query: 481 QE 482
            E
Sbjct: 481 VE 476

BLAST of Spg021830 vs. NCBI nr
Match: KAG6588239.1 (putative polyamine transporter, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022154.1 putative polyamine transporter, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 769.2 bits (1985), Expect = 2.1e-218
Identity = 385/483 (79.71%), Postives = 431/483 (89.23%), Query Frame = 0

Query: 1   MADQKQELKCNHNHNQSTADSSSISQDLPTT-TTATAPTAASNKLTLIPLIFIIYFQVSG 60
           MAD+KQ L  + N +Q  ADSSS SQ LPTT  TAT+  AA  KLTLIPLIF+IYF+V+G
Sbjct: 1   MADKKQSLNKSTN-SQPLADSSSPSQTLPTTVATATSSAAARKKLTLIPLIFLIYFEVAG 60

Query: 61  GPYDEESAVQAAGPLLTIIGFLVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGPF 120
           GPY EE AVQAAGPLL I+GF+VFPFIWSVPEALITAELST FPGDGG+VIWA+RAFGPF
Sbjct: 61  GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPF 120

Query: 121 WGSLIGTWKLLSGIINITACPVLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIGL 180
           WGSL+GTWKLLSG+INI A PVLC++Y+KKIA PLESGWPRR A+  STLLL+LLNY+GL
Sbjct: 121 WGSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFGSTLLLALLNYVGL 180

Query: 181 TIVGYVAVVLAFLSLLPFMIMTFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYW 240
           TIVGYVAVVLAFLSLLPF++MTF+AIP + P RWLS GDKGVKKDWNLYLNT+FWNLN+W
Sbjct: 181 TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFW 240

Query: 241 DNVSTLAGEVQNPKKTFPIALFVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAAE 300
           DNVSTLAGEV+NP+KTFP+ALFVSVIFTC+SYLIP+LAV+GAVDVEQS W SGFHAQAA 
Sbjct: 241 DNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAG 300

Query: 301 TIAGGKWLKILLEIGECLSAIGLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPWI 360
            IA GKWLKILLEIG CLS IGLFEAQLSSSAYQILGMAEIGILPK  A RAKWFNTPWI
Sbjct: 301 IIA-GKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWI 360

Query: 361 GIVICTAISLAVSYMNFDDIVATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLP 420
           GIVICTAISLAVSYMNF DIVA+ANFIYSLGMLLEFS+F+WLRWKHPG+KRPF+VPL+LP
Sbjct: 361 GIVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLP 420

Query: 421 GLIVMCLVSSAFFVVVMYFTHKKVFLVSAAMTAAGVIWFELMKICKKKEIFEFNPRPEAI 480
            LIVMCL+ SAF VV+M FTHK V +VSA MTAAG++WF +MKICK K+I EFNP  +AI
Sbjct: 421 ALIVMCLIPSAFLVVLMVFTHKMVLVVSAVMTAAGIVWFGVMKICKNKKILEFNPPLDAI 480

Query: 481 QES 483
           +ES
Sbjct: 481 EES 481

BLAST of Spg021830 vs. NCBI nr
Match: XP_022933749.1 (probable polyamine transporter At3g13620 [Cucurbita moschata])

HSP 1 Score: 766.5 bits (1978), Expect = 1.3e-217
Identity = 384/483 (79.50%), Postives = 431/483 (89.23%), Query Frame = 0

Query: 1   MADQKQELKCNHNHNQSTADSSSISQDLPTT-TTATAPTAASNKLTLIPLIFIIYFQVSG 60
           MAD+KQ    + N +Q  ADSSS SQ LPTT  TAT+  AA  KLTLIPLIF+IYF+V+G
Sbjct: 1   MADKKQSPNKSTN-SQPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAG 60

Query: 61  GPYDEESAVQAAGPLLTIIGFLVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGPF 120
           GPY EE AVQAAGPLL I+GF+VFPFIWSVPEALITAELST FPGDGG+VIWA+RAFGPF
Sbjct: 61  GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPF 120

Query: 121 WGSLIGTWKLLSGIINITACPVLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIGL 180
           WGSL+GTWKLLSG+INI A PVLC++Y+KKIA PLESGWPRR A+ +STLLL+LLNY+GL
Sbjct: 121 WGSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFSSTLLLALLNYVGL 180

Query: 181 TIVGYVAVVLAFLSLLPFMIMTFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYW 240
           TIVGYVAVVLAFLSLLPF++MTF+AIP + P RWLS GDKGVKKDWNLYLNT+FWNLN+W
Sbjct: 181 TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFW 240

Query: 241 DNVSTLAGEVQNPKKTFPIALFVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAAE 300
           DNVSTLAGEV+NP+KTFP+ALFVSVIFTC+SYLIP+LAV+GAVDVEQS W SGFHAQAA 
Sbjct: 241 DNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAG 300

Query: 301 TIAGGKWLKILLEIGECLSAIGLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPWI 360
            IA GKWLKILLEIG CLS IGLFEAQLSSSAYQILGMAEIGILPK  A RAKWFNTPWI
Sbjct: 301 IIA-GKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWI 360

Query: 361 GIVICTAISLAVSYMNFDDIVATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLP 420
           GIVICTAISLAVSYMNF DIVA+ANFIYSLGMLLEFS+F+WLRWKHPG+KRPF+VPL+LP
Sbjct: 361 GIVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLP 420

Query: 421 GLIVMCLVSSAFFVVVMYFTHKKVFLVSAAMTAAGVIWFELMKICKKKEIFEFNPRPEAI 480
            LIVMCL+ SAF VV+M FTHK V +VSA MTAAG++WF +MKICK K+I EFNP  +AI
Sbjct: 421 ALIVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGVMKICKNKKILEFNPPLDAI 480

Query: 481 QES 483
           +ES
Sbjct: 481 EES 481

BLAST of Spg021830 vs. NCBI nr
Match: XP_023006107.1 (probable polyamine transporter At3g13620 [Cucurbita maxima])

HSP 1 Score: 765.8 bits (1976), Expect = 2.3e-217
Identity = 385/484 (79.55%), Postives = 434/484 (89.67%), Query Frame = 0

Query: 1   MADQKQELKCNHNHNQSTADSSSISQDLPTTT-TATAPTAAS-NKLTLIPLIFIIYFQVS 60
           MAD+KQ    + N +Q  ADSSS SQ LPTT+ TAT+  AAS  KLTLIPLIF+IYF+V+
Sbjct: 1   MADKKQSPNKSTN-SQPLADSSSTSQTLPTTSATATSSAAASRKKLTLIPLIFLIYFEVA 60

Query: 61  GGPYDEESAVQAAGPLLTIIGFLVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGP 120
           GGPY EE AVQAAGPLL I+GF+VFPFIWSVPEALITAELST FPGDGG+VIWA+RAFGP
Sbjct: 61  GGPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGP 120

Query: 121 FWGSLIGTWKLLSGIINITACPVLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIG 180
           FWGSL+GTWKLLSG+INI A PVLC++Y+KKIA PLESGWPRR A+ +STLLL+LLNY+G
Sbjct: 121 FWGSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFSSTLLLALLNYVG 180

Query: 181 LTIVGYVAVVLAFLSLLPFMIMTFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNY 240
           LTIVGYVAVVLAFLSLLPF++MTF+AIP + P RWLSLGDKGVKKDWNLYLNT+FWNLN+
Sbjct: 181 LTIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNF 240

Query: 241 WDNVSTLAGEVQNPKKTFPIALFVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAA 300
           WDNVSTLAGEV+NP+KTFP+ALFVSVIFTC+SYLIP+LAV+GAVDVEQS W SGFHAQAA
Sbjct: 241 WDNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAA 300

Query: 301 ETIAGGKWLKILLEIGECLSAIGLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPW 360
             IA GKWLKILLEIG CLS IGLFEAQLSSSAYQILGMAEIGILPK  A RAKWFNTPW
Sbjct: 301 GIIA-GKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFATRAKWFNTPW 360

Query: 361 IGIVICTAISLAVSYMNFDDIVATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKL 420
           IGIVICTAISLAVSYMNF DIVA+ANFIYSLGMLLEFS+F+WLRWKHPG+KRPF+VPL+L
Sbjct: 361 IGIVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRL 420

Query: 421 PGLIVMCLVSSAFFVVVMYFTHKKVFLVSAAMTAAGVIWFELMKICKKKEIFEFNPRPEA 480
           P LI+MCL+ SAF VV+M FTHK V +VSA MTAAG++WF LMK+CK K+I EFNP  +A
Sbjct: 421 PALILMCLIPSAFLVVLMVFTHKMVLVVSAVMTAAGIVWFGLMKVCKNKKILEFNPPLDA 480

Query: 481 IQES 483
           I+ES
Sbjct: 481 IEES 482

BLAST of Spg021830 vs. NCBI nr
Match: XP_023529220.1 (probable polyamine transporter At3g13620 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 763.8 bits (1971), Expect = 8.6e-217
Identity = 382/483 (79.09%), Postives = 429/483 (88.82%), Query Frame = 0

Query: 1   MADQKQELKCNHNHNQSTADSSSISQDLPTT-TTATAPTAASNKLTLIPLIFIIYFQVSG 60
           MAD+KQ    + N +   ADSSS SQ LPTT  TAT+  AA  KLTLIPLIF+IYF+V+G
Sbjct: 1   MADKKQSPNKSTN-SHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAG 60

Query: 61  GPYDEESAVQAAGPLLTIIGFLVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGPF 120
           GPY EE AVQAAGPLL I+GF+VFPFIWSVPEALITAELST FPGDGG+VIWA+RAFGPF
Sbjct: 61  GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPF 120

Query: 121 WGSLIGTWKLLSGIINITACPVLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIGL 180
           WGSL+GTWKLLSG+INI A PVLC++Y+KKI  PLESGWPRR A+ +STLLL+LLNY+GL
Sbjct: 121 WGSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGL 180

Query: 181 TIVGYVAVVLAFLSLLPFMIMTFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYW 240
           TIVGYVAVVLAFLSLLPF++MTF+AIP + P RWLS GDKGVKKDWNLYLNT+FWNLN+W
Sbjct: 181 TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFW 240

Query: 241 DNVSTLAGEVQNPKKTFPIALFVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAAE 300
           DNVSTLAGEV+NP+KTFP+ALFVSVIFTC+SYLIP+LAV+GAVDVEQS W SGFHAQAA 
Sbjct: 241 DNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAG 300

Query: 301 TIAGGKWLKILLEIGECLSAIGLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPWI 360
            IA GKWLKILLEIG CLS IGLFEAQLSSSAYQILGMAEIGILPK  A RAKWFNTPWI
Sbjct: 301 IIA-GKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWI 360

Query: 361 GIVICTAISLAVSYMNFDDIVATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLP 420
           GIVICTAISLAVSYMNF DIVA+ANFIYSLGMLLEFS+F+WLRWKHPG+KRPF+VPL+LP
Sbjct: 361 GIVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLP 420

Query: 421 GLIVMCLVSSAFFVVVMYFTHKKVFLVSAAMTAAGVIWFELMKICKKKEIFEFNPRPEAI 480
            LIVMCL+ SAF VV+M FTHK V +VSA MTAAG++WF LMK+CK K+I EFNP  +AI
Sbjct: 421 ALIVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAI 480

Query: 481 QES 483
           +ES
Sbjct: 481 EES 481

BLAST of Spg021830 vs. ExPASy Swiss-Prot
Match: Q9LHN7 (Probable polyamine transporter At3g13620 OS=Arabidopsis thaliana OX=3702 GN=At3g13620 PE=2 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 5.9e-168
Identity = 288/456 (63.16%), Postives = 365/456 (80.04%), Query Frame = 0

Query: 19  ADSSSISQDLPTTTT-ATAPTAASNKLTLIPLIFIIYFQVSGGPYDEESAVQAAGPLLTI 78
           +++S  S +LP TT  ++   A + KLTLIPL+F+IYF+V+GGP+ EE AVQAAGPLL I
Sbjct: 4   SEASKSSHELPVTTAESSGKKATAKKLTLIPLVFLIYFEVAGGPFGEEPAVQAAGPLLAI 63

Query: 79  IGFLVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGPFWGSLIGTWKLLSGIINIT 138
           +GFL+FPFIWS+PEALITAELST FPG+GG+VIWA RAFG F GS++G+ K LSG+IN+ 
Sbjct: 64  LGFLIFPFIWSIPEALITAELSTAFPGNGGFVIWAHRAFGSFVGSMMGSLKFLSGVINVA 123

Query: 139 ACPVLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIGLTIVGYVAVVLAFLSLLPF 198
           + PVLCV Y+ K+   LESGWPR   I AST++LS LNY GL IVGY AVVL  +SL PF
Sbjct: 124 SFPVLCVTYLDKLFPVLESGWPRNVCIFASTVVLSFLNYTGLAIVGYAAVVLGLVSLSPF 183

Query: 199 MIMTFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYWDNVSTLAGEVQNPKKTFP 258
           ++M+ MAIP ++P RW SLG K  KKDWNLY NT+FWNLN+WDNVSTLAGEV  P+KTFP
Sbjct: 184 LVMSAMAIPKIKPHRWGSLGTK--KKDWNLYFNTLFWNLNFWDNVSTLAGEVDEPQKTFP 243

Query: 259 IALFVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAAETIAGGKWLKILLEIGECL 318
           +AL ++VIFTC++YLIP+ AV GAV V+QS WE+GFHA+AAE IA GKWLKI +EIG  L
Sbjct: 244 LALLIAVIFTCVAYLIPLFAVTGAVSVDQSRWENGFHAEAAEMIA-GKWLKIWIEIGAVL 303

Query: 319 SAIGLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPWIGIVICTAISLAVSYMNFD 378
           S+IGLFEAQLSSSAYQ+ GMAE+G LPK    R+KWFNTPW+GI+I   +SL +SYMNF 
Sbjct: 304 SSIGLFEAQLSSSAYQLEGMAELGFLPKFFGVRSKWFNTPWVGILISALMSLGLSYMNFT 363

Query: 379 DIVATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMCLVSSAFFVVVMY 438
           DI+++ANF+Y+LGM LEF+SF+WLR K P +KRP++VPLK+PGL+VMCL+ SAF V+++ 
Sbjct: 364 DIISSANFLYTLGMFLEFASFIWLRRKLPQLKRPYRVPLKIPGLVVMCLIPSAFLVLILV 423

Query: 439 FTHKKVFLVSAAMTAAGVIWFELMKICKKKEIFEFN 474
           F  K V+L+   MT   + W+ L+   +K +IFEFN
Sbjct: 424 FATKIVYLICGVMTIGAIGWYFLINYFRKTKIFEFN 456

BLAST of Spg021830 vs. ExPASy Swiss-Prot
Match: Q9FFL1 (Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.2e-112
Identity = 209/453 (46.14%), Postives = 305/453 (67.33%), Query Frame = 0

Query: 24  ISQDLPTTTTATAPTAASN---KLTLIPLIFIIYFQVSGGPYDEESAVQAAGPLLTIIGF 83
           IS  + T   AT+P    N   K+T++PL+F+I+++VSGGP+  E +V+AAGPLL I+GF
Sbjct: 30  ISIGVTTGDPATSPARTVNQIKKITVLPLVFLIFYEVSGGPFGIEDSVKAAGPLLAIVGF 89

Query: 84  LVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGPFWGSLIGTWKLLSGIINITACP 143
           +VFPFIWS+PEALITAE+ T FP +GGYV+W   A GP+WG   G  K LSG+I+    P
Sbjct: 90  IVFPFIWSIPEALITAEMGTMFPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYP 149

Query: 144 VLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIGLTIVGYVAVVLAFLSLLPFMIM 203
           +L ++Y+K     L SG PR  AIL  T+ L+ LNY GL+IVG  AV+L   S+LPF++M
Sbjct: 150 ILFLDYLKSGIPILGSGIPRVAAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVM 209

Query: 204 TFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYWDNVSTLAGEVQNPKKTFPIAL 263
           +FM+IP ++P RWL +  K    +W+LYLNT+FWNLNYWD+VSTL GEV+NP KT P AL
Sbjct: 210 SFMSIPKLKPSRWLVVSKKMKGVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRAL 269

Query: 264 FVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAAETIAGGKWLKILLEIGECLSAI 323
           F +++    SY+ P+L   GA+ ++Q  W  G+ A   + I GG WL   ++     S +
Sbjct: 270 FYALLLVVFSYIFPVLTGTGAIALDQKLWTDGYFADIGKVI-GGVWLGWWIQAAAATSNM 329

Query: 324 GLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPWIGIVICTAISLAVSYMNFDDIV 383
           G+F A++SS ++Q+LGMAE G+LP++ A R++ + TPW+GI+   +  + +S+++F +IV
Sbjct: 330 GMFLAEMSSDSFQLLGMAERGMLPEVFAKRSR-YRTPWVGILFSASGVIILSWLSFQEIV 389

Query: 384 ATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMCLVSSAFFVVVMYFTH 443
           A  N +Y  GM+LEF +FV LR K+P   RPFK+P+ + G ++MC+  +    V+M FT+
Sbjct: 390 AAENLLYCFGMVLEFITFVRLRMKYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTN 449

Query: 444 KKVFLVSAAMTAAGVIWFELMKICKKKEIFEFN 474
            KV LVS A    G++    +K  +KK   +F+
Sbjct: 450 LKVALVSLAAIVIGLVLQPCLKQVEKKGWLKFS 480

BLAST of Spg021830 vs. ExPASy Swiss-Prot
Match: A2X8M8 (Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 6.9e-108
Identity = 208/450 (46.22%), Postives = 291/450 (64.67%), Query Frame = 0

Query: 28  LPTTTTATAPTAASNKLTLIPLIFIIYFQVSGGPYDEESAVQAAGPLLTIIGFLVFPFIW 87
           LP         +++  +++IPLIF+I+++VSGGP+  E +V AAGPLL IIGFLV P IW
Sbjct: 67  LPDGDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGIEDSVGAAGPLLAIIGFLVLPVIW 126

Query: 88  SVPEALITAELSTTFPGDGGYVIWAERAFGPFWGSLIGTWKLLSGIINITACPVLCVEYM 147
           S+PEALITAEL   FP +GGYV+W   A GP+WG   G  K LSG+I+    PVL ++Y+
Sbjct: 127 SIPEALITAELGAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYL 186

Query: 148 KKIATPLESGWPRRTAILASTLLLSLLNYIGLTIVGYVAVVLAFLSLLPFMIMTFMAIPM 207
           K     L  G PR  A++  T +L+LLNY GLT+VG+VA+ L   SLLPF +M  +A+P 
Sbjct: 187 KSGVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPK 246

Query: 208 VRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYWDNVSTLAGEVQNPKKTFPIALFVSVIFT 267
           +RP RWL +    V  DWNLYLNT+FWNLNYWD++STLAGEV+NP KT P ALF +VIF 
Sbjct: 247 LRPARWLVIDLHNV--DWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFV 306

Query: 268 CLSYLIPILAVVGAVDVEQSTWESGFHAQAAETIAGGKWLKILLEIGECLSAIGLFEAQL 327
            ++YL P+LA  GAV +++  W  G+ A  A+ + GG WL   ++    LS +G+F A++
Sbjct: 307 VVAYLYPLLAGTGAVPLDRGQWTDGYFADIAK-LLGGAWLMWWVQSAAALSNMGMFVAEM 366

Query: 328 SSSAYQILGMAEIGILPKLLAGRAKWFNTPWIGIVICTAISLAVSYMNFDDIVATANFIY 387
           SS +YQ+LGMAE G+LP   A R++ + TP  GI+   +  L +S M+F +IVA  NF+Y
Sbjct: 367 SSDSYQLLGMAERGMLPSFFAARSR-YGTPLAGILFSASGVLLLSMMSFQEIVAAENFLY 426

Query: 388 SLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMCLVSSAFFVVVMYFTHKKVFLVS 447
             GMLLEF +F+  R + P   RP++VPL   G + M +  +A   VV+  +  KV +VS
Sbjct: 427 CFGMLLEFVAFILHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVS 486

Query: 448 AAMTAAGVIWFELMKICKKKEIFEFNPRPE 478
               A G++    ++  +KK    F+  P+
Sbjct: 487 LGAVAMGLVLQPALRFVEKKRWLRFSVNPD 512

BLAST of Spg021830 vs. ExPASy Swiss-Prot
Match: Q6Z8D0 (Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 6.9e-108
Identity = 208/450 (46.22%), Postives = 291/450 (64.67%), Query Frame = 0

Query: 28  LPTTTTATAPTAASNKLTLIPLIFIIYFQVSGGPYDEESAVQAAGPLLTIIGFLVFPFIW 87
           LP         +++  +++IPLIF+I+++VSGGP+  E +V AAGPLL IIGFLV P IW
Sbjct: 67  LPDGDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGIEDSVGAAGPLLAIIGFLVLPVIW 126

Query: 88  SVPEALITAELSTTFPGDGGYVIWAERAFGPFWGSLIGTWKLLSGIINITACPVLCVEYM 147
           S+PEALITAEL   FP +GGYV+W   A GP+WG   G  K LSG+I+    PVL ++Y+
Sbjct: 127 SIPEALITAELGAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYL 186

Query: 148 KKIATPLESGWPRRTAILASTLLLSLLNYIGLTIVGYVAVVLAFLSLLPFMIMTFMAIPM 207
           K     L  G PR  A++  T +L+LLNY GLT+VG+VA+ L   SLLPF +M  +A+P 
Sbjct: 187 KSGVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPK 246

Query: 208 VRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYWDNVSTLAGEVQNPKKTFPIALFVSVIFT 267
           +RP RWL +    V  DWNLYLNT+FWNLNYWD++STLAGEV+NP KT P ALF +VIF 
Sbjct: 247 LRPARWLVIDLHNV--DWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFV 306

Query: 268 CLSYLIPILAVVGAVDVEQSTWESGFHAQAAETIAGGKWLKILLEIGECLSAIGLFEAQL 327
            ++YL P+LA  GAV +++  W  G+ A  A+ + GG WL   ++    LS +G+F A++
Sbjct: 307 VVAYLYPLLAGTGAVPLDRGQWTDGYFADIAK-LLGGAWLMWWVQSAAALSNMGMFVAEM 366

Query: 328 SSSAYQILGMAEIGILPKLLAGRAKWFNTPWIGIVICTAISLAVSYMNFDDIVATANFIY 387
           SS +YQ+LGMAE G+LP   A R++ + TP  GI+   +  L +S M+F +IVA  NF+Y
Sbjct: 367 SSDSYQLLGMAERGMLPSFFAARSR-YGTPLAGILFSASGVLLLSMMSFQEIVAAENFLY 426

Query: 388 SLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMCLVSSAFFVVVMYFTHKKVFLVS 447
             GMLLEF +F+  R + P   RP++VPL   G + M +  +A   VV+  +  KV +VS
Sbjct: 427 CFGMLLEFVAFILHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVS 486

Query: 448 AAMTAAGVIWFELMKICKKKEIFEFNPRPE 478
               A G++    ++  +KK    F+  P+
Sbjct: 487 LGAVAMGLVLQPALRFVEKKRWLRFSVNPD 512

BLAST of Spg021830 vs. ExPASy Swiss-Prot
Match: Q9LH39 (Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g19553 PE=3 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 7.6e-107
Identity = 205/436 (47.02%), Postives = 288/436 (66.06%), Query Frame = 0

Query: 41  SNKLTLIPLIFIIYFQVSGGPYDEESAVQA-AGPLLTIIGFLVFPFIWSVPEALITAELS 100
           S KLTL+PL+F+I+++VSGGP+  E +V++  GPLL ++GFL+FP IWS+PEAL+TAEL+
Sbjct: 19  SPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWSIPEALVTAELA 78

Query: 101 TTFPGDGGYVIWAERAFGPFWGSLIGTWKLLSGIINITACPVLCVEYMKKIATPLESGWP 160
           T+FP +GGYV+W   AFGPFWG   G WK  SG+++    PVL ++Y+K     L+    
Sbjct: 79  TSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKHSFPVLDHVAA 138

Query: 161 RRTAILASTLLLSLLNYIGLTIVGYVAVVLAFLSLLPFMIMTFMAIPMVRPRRWLSLGDK 220
           R  A+L  T  L+ LNY GL IVG+ AVVLA  SL PF++M  +A+P +RP+RWL +  +
Sbjct: 139 RVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNIRPKRWLFVDTQ 198

Query: 221 GVKKDWNLYLNTMFWNLNYWDNVSTLAGEVQNPKKTFPIALFVSVIFTCLSYLIPILAVV 280
             K +W  Y NTMFWNLNYWD  STLAGEV  P KTFP ALF +V+    SYLIP++A  
Sbjct: 199 --KINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGSYLIPLMAGT 258

Query: 281 GAVDVEQS-TWESGFHAQAAETIAGGKWLKILLEIGECLSAIGLFEAQLSSSAYQILGMA 340
           GA+    S  W  G+ A+    I GG WLK  ++    +S +GLFEA++SS A+Q+LGM+
Sbjct: 259 GALSSSTSGEWSDGYFAEVGMLI-GGVWLKGWIQAAAAMSNLGLFEAEMSSDAFQLLGMS 318

Query: 341 EIGILPKLLAGRAKWFNTPWIGIVICTAISLAVSYMNFDDIVATANFIYSLGMLLEFSSF 400
           EIG+LP   A R+K + TP I I+      + +S+M+F +I+   NF+Y+LGMLLEF++F
Sbjct: 319 EIGMLPAFFAQRSK-YGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGMLLEFAAF 378

Query: 401 VWLRWKHPGMKRPFKVPLKLPGLIVMCLVSSAFFVVVMYFTHKKVFLVSAAMTAAGVIWF 460
           V LR K P + RP++VPL   G+ ++CL  S   ++VM     K FL+S  +   G   +
Sbjct: 379 VKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVIIVLGFCLY 438

Query: 461 ELMKICKKKEIFEFNP 475
             + + K+K+   F P
Sbjct: 439 PFLTLVKEKQWARFIP 450

BLAST of Spg021830 vs. ExPASy TrEMBL
Match: A0A6J1F0M1 (probable polyamine transporter At3g13620 OS=Cucurbita moschata OX=3662 GN=LOC111441072 PE=3 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 6.5e-218
Identity = 384/483 (79.50%), Postives = 431/483 (89.23%), Query Frame = 0

Query: 1   MADQKQELKCNHNHNQSTADSSSISQDLPTT-TTATAPTAASNKLTLIPLIFIIYFQVSG 60
           MAD+KQ    + N +Q  ADSSS SQ LPTT  TAT+  AA  KLTLIPLIF+IYF+V+G
Sbjct: 1   MADKKQSPNKSTN-SQPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAG 60

Query: 61  GPYDEESAVQAAGPLLTIIGFLVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGPF 120
           GPY EE AVQAAGPLL I+GF+VFPFIWSVPEALITAELST FPGDGG+VIWA+RAFGPF
Sbjct: 61  GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPF 120

Query: 121 WGSLIGTWKLLSGIINITACPVLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIGL 180
           WGSL+GTWKLLSG+INI A PVLC++Y+KKIA PLESGWPRR A+ +STLLL+LLNY+GL
Sbjct: 121 WGSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFSSTLLLALLNYVGL 180

Query: 181 TIVGYVAVVLAFLSLLPFMIMTFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYW 240
           TIVGYVAVVLAFLSLLPF++MTF+AIP + P RWLS GDKGVKKDWNLYLNT+FWNLN+W
Sbjct: 181 TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFW 240

Query: 241 DNVSTLAGEVQNPKKTFPIALFVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAAE 300
           DNVSTLAGEV+NP+KTFP+ALFVSVIFTC+SYLIP+LAV+GAVDVEQS W SGFHAQAA 
Sbjct: 241 DNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAG 300

Query: 301 TIAGGKWLKILLEIGECLSAIGLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPWI 360
            IA GKWLKILLEIG CLS IGLFEAQLSSSAYQILGMAEIGILPK  A RAKWFNTPWI
Sbjct: 301 IIA-GKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWI 360

Query: 361 GIVICTAISLAVSYMNFDDIVATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLP 420
           GIVICTAISLAVSYMNF DIVA+ANFIYSLGMLLEFS+F+WLRWKHPG+KRPF+VPL+LP
Sbjct: 361 GIVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLP 420

Query: 421 GLIVMCLVSSAFFVVVMYFTHKKVFLVSAAMTAAGVIWFELMKICKKKEIFEFNPRPEAI 480
            LIVMCL+ SAF VV+M FTHK V +VSA MTAAG++WF +MKICK K+I EFNP  +AI
Sbjct: 421 ALIVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGVMKICKNKKILEFNPPLDAI 480

Query: 481 QES 483
           +ES
Sbjct: 481 EES 481

BLAST of Spg021830 vs. ExPASy TrEMBL
Match: A0A6J1KV06 (probable polyamine transporter At3g13620 OS=Cucurbita maxima OX=3661 GN=LOC111498946 PE=3 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 1.1e-217
Identity = 385/484 (79.55%), Postives = 434/484 (89.67%), Query Frame = 0

Query: 1   MADQKQELKCNHNHNQSTADSSSISQDLPTTT-TATAPTAAS-NKLTLIPLIFIIYFQVS 60
           MAD+KQ    + N +Q  ADSSS SQ LPTT+ TAT+  AAS  KLTLIPLIF+IYF+V+
Sbjct: 1   MADKKQSPNKSTN-SQPLADSSSTSQTLPTTSATATSSAAASRKKLTLIPLIFLIYFEVA 60

Query: 61  GGPYDEESAVQAAGPLLTIIGFLVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGP 120
           GGPY EE AVQAAGPLL I+GF+VFPFIWSVPEALITAELST FPGDGG+VIWA+RAFGP
Sbjct: 61  GGPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGP 120

Query: 121 FWGSLIGTWKLLSGIINITACPVLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIG 180
           FWGSL+GTWKLLSG+INI A PVLC++Y+KKIA PLESGWPRR A+ +STLLL+LLNY+G
Sbjct: 121 FWGSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFSSTLLLALLNYVG 180

Query: 181 LTIVGYVAVVLAFLSLLPFMIMTFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNY 240
           LTIVGYVAVVLAFLSLLPF++MTF+AIP + P RWLSLGDKGVKKDWNLYLNT+FWNLN+
Sbjct: 181 LTIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNF 240

Query: 241 WDNVSTLAGEVQNPKKTFPIALFVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAA 300
           WDNVSTLAGEV+NP+KTFP+ALFVSVIFTC+SYLIP+LAV+GAVDVEQS W SGFHAQAA
Sbjct: 241 WDNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAA 300

Query: 301 ETIAGGKWLKILLEIGECLSAIGLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPW 360
             IA GKWLKILLEIG CLS IGLFEAQLSSSAYQILGMAEIGILPK  A RAKWFNTPW
Sbjct: 301 GIIA-GKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFATRAKWFNTPW 360

Query: 361 IGIVICTAISLAVSYMNFDDIVATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKL 420
           IGIVICTAISLAVSYMNF DIVA+ANFIYSLGMLLEFS+F+WLRWKHPG+KRPF+VPL+L
Sbjct: 361 IGIVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRL 420

Query: 421 PGLIVMCLVSSAFFVVVMYFTHKKVFLVSAAMTAAGVIWFELMKICKKKEIFEFNPRPEA 480
           P LI+MCL+ SAF VV+M FTHK V +VSA MTAAG++WF LMK+CK K+I EFNP  +A
Sbjct: 421 PALILMCLIPSAFLVVLMVFTHKMVLVVSAVMTAAGIVWFGLMKVCKNKKILEFNPPLDA 480

Query: 481 IQES 483
           I+ES
Sbjct: 481 IEES 482

BLAST of Spg021830 vs. ExPASy TrEMBL
Match: A0A0A0M300 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G630310 PE=3 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 2.2e-210
Identity = 376/481 (78.17%), Postives = 421/481 (87.53%), Query Frame = 0

Query: 1   MADQKQELKCNHNHNQSTADSSSISQDLPTTTTATAPTAASNKLTLIPLIFIIYFQVSGG 60
           MAD KQ+   ++++  +T DSS  SQ LPTTT +T P +   KLTLIPLIF+IYF+V+GG
Sbjct: 1   MADDKQQ---SNSNQPATTDSS--SQILPTTTPST-PPSVRKKLTLIPLIFLIYFEVAGG 60

Query: 61  PYDEESAVQAAGPLLTIIGFLVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGPFW 120
           PY EE  VQAAGPLL IIGF+VFPFIWSVPEALITAELST FPG+GG+VIWAERAFGPFW
Sbjct: 61  PYGEEPTVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFW 120

Query: 121 GSLIGTWKLLSGIINITACPVLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIGLT 180
           GSL+GTWKLLSG+INI A PVLC++Y+KKIA PLESGWPRR A+LASTL+L+ LNYIGLT
Sbjct: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVLASTLILAALNYIGLT 180

Query: 181 IVGYVAVVLAFLSLLPFMIMTFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYWD 240
           IVGYVAVVLAFLSLLPF++MTF+AIP ++P RWL LGDK  ++DWNLYLNT+FWNLN+WD
Sbjct: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIKPHRWLILGDK--ERDWNLYLNTLFWNLNFWD 240

Query: 241 NVSTLAGEVQNPKKTFPIALFVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAAET 300
           NVSTLAGEV+NP+KTFP ALFVSVIFTCLSYLIP+LAV+GAVDVEQS W SGFHAQAA  
Sbjct: 241 NVSTLAGEVENPQKTFPKALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300

Query: 301 IAGGKWLKILLEIGECLSAIGLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPWIG 360
           IA GKWLK LLEIG  LSAIGLFEAQLSSSAYQILGMAEIG+LPK    RAKWF TPWIG
Sbjct: 301 IA-GKWLKFLLEIGSTLSAIGLFEAQLSSSAYQILGMAEIGVLPKFFGSRAKWFKTPWIG 360

Query: 361 IVICTAISLAVSYMNFDDIVATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPG 420
           IVICTAISLAVSYM+F DIVA+ANFIYSLGMLLEFSSFVWLRW+HPG+KRPFKVPLKLPG
Sbjct: 361 IVICTAISLAVSYMDFTDIVASANFIYSLGMLLEFSSFVWLRWRHPGIKRPFKVPLKLPG 420

Query: 421 LIVMCLVSSAFFVVVMYFTHKKVFLVSAAMTAAGVIWFELMKICKKKEIFEFNPRPEAIQ 480
           LI+MCL+ S F VVVM FTHK V LVS  MT  G++WF LMKICKKK+I EFNP PEAI 
Sbjct: 421 LIMMCLIPSGFLVVVMVFTHKNVLLVSGGMTVGGILWFGLMKICKKKKILEFNPEPEAIV 472

Query: 481 E 482
           E
Sbjct: 481 E 472

BLAST of Spg021830 vs. ExPASy TrEMBL
Match: A0A5A7UQT6 (Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold289G00460 PE=3 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 6.5e-210
Identity = 376/481 (78.17%), Postives = 419/481 (87.11%), Query Frame = 0

Query: 1   MADQKQELKCNHNHNQSTADSSSISQDLPTTTTATAPTAASNKLTLIPLIFIIYFQVSGG 60
           MAD KQ    +++    T DSS  SQ LPTTT +T P A   KLTLIPLIF+IYF+V+GG
Sbjct: 1   MADDKQ----SNSSLPPTTDSS--SQILPTTTPST-PAAVRKKLTLIPLIFLIYFEVAGG 60

Query: 61  PYDEESAVQAAGPLLTIIGFLVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGPFW 120
           PY EE  VQAAGPLL I+GF++FPFIWSVPEALITAELST FPG+GG+VIWAERAFGPFW
Sbjct: 61  PYGEEPTVQAAGPLLAIVGFILFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFW 120

Query: 121 GSLIGTWKLLSGIINITACPVLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIGLT 180
           GSL+GTWKLLSG+INI A PVLC++Y+KKIA PLESGWPRR A+LASTL+L+LLNYIGLT
Sbjct: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVLASTLILALLNYIGLT 180

Query: 181 IVGYVAVVLAFLSLLPFMIMTFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYWD 240
           IVGYVAVVLAFLSLLPF++MTF+AIP ++P RWL LGDK  ++DWNLYLNT+FWNLN+WD
Sbjct: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIKPHRWLILGDK--ERDWNLYLNTLFWNLNFWD 240

Query: 241 NVSTLAGEVQNPKKTFPIALFVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAAET 300
           NVSTLAGEV+NP+KTFP ALFVSVIFTCLSYL+P+LAV+GAVDVEQS W SGFHAQAA  
Sbjct: 241 NVSTLAGEVENPQKTFPKALFVSVIFTCLSYLVPLLAVIGAVDVEQSAWGSGFHAQAAGI 300

Query: 301 IAGGKWLKILLEIGECLSAIGLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPWIG 360
           IA GKWLKILLEIG  LSAIGLFEAQLSSSAYQILGMAEIG+LPK    RAKWF TPWIG
Sbjct: 301 IA-GKWLKILLEIGSTLSAIGLFEAQLSSSAYQILGMAEIGVLPKFFGSRAKWFKTPWIG 360

Query: 361 IVICTAISLAVSYMNFDDIVATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPG 420
           IVICTAISLAVSYM+F DIVA+ANFIYSLGMLLE SSFVWLRWKHPG+KRPFKVPLKLPG
Sbjct: 361 IVICTAISLAVSYMDFTDIVASANFIYSLGMLLELSSFVWLRWKHPGIKRPFKVPLKLPG 420

Query: 421 LIVMCLVSSAFFVVVMYFTHKKVFLVSAAMTAAGVIWFELMKICKKKEIFEFNPRPEAIQ 480
           LIVMCL+ + F VVVM FTHK V LVS  MT  G++WF LMKICKKK+I EFNP PEAI 
Sbjct: 421 LIVMCLIPAGFLVVVMVFTHKNVLLVSVGMTVGGILWFGLMKICKKKKILEFNPEPEAIV 471

Query: 481 E 482
           E
Sbjct: 481 E 471

BLAST of Spg021830 vs. ExPASy TrEMBL
Match: A0A1S3BNB6 (probable polyamine transporter At3g13620 OS=Cucumis melo OX=3656 GN=LOC103491953 PE=3 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 6.5e-210
Identity = 376/481 (78.17%), Postives = 419/481 (87.11%), Query Frame = 0

Query: 1   MADQKQELKCNHNHNQSTADSSSISQDLPTTTTATAPTAASNKLTLIPLIFIIYFQVSGG 60
           MAD KQ    +++    T DSS  SQ LPTTT +T P A   KLTLIPLIF+IYF+V+GG
Sbjct: 1   MADDKQ----SNSSLPPTTDSS--SQILPTTTPST-PAAVRKKLTLIPLIFLIYFEVAGG 60

Query: 61  PYDEESAVQAAGPLLTIIGFLVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGPFW 120
           PY EE  VQAAGPLL I+GF++FPFIWSVPEALITAELST FPG+GG+VIWAERAFGPFW
Sbjct: 61  PYGEEPTVQAAGPLLAIVGFILFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFW 120

Query: 121 GSLIGTWKLLSGIINITACPVLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIGLT 180
           GSL+GTWKLLSG+INI A PVLC++Y+KKIA PLESGWPRR A+LASTL+L+LLNYIGLT
Sbjct: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVLASTLILALLNYIGLT 180

Query: 181 IVGYVAVVLAFLSLLPFMIMTFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYWD 240
           IVGYVAVVLAFLSLLPF++MTF+AIP ++P RWL LGDK  ++DWNLYLNT+FWNLN+WD
Sbjct: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIKPHRWLILGDK--ERDWNLYLNTLFWNLNFWD 240

Query: 241 NVSTLAGEVQNPKKTFPIALFVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAAET 300
           NVSTLAGEV+NP+KTFP ALFVSVIFTCLSYL+P+LAV+GAVDVEQS W SGFHAQAA  
Sbjct: 241 NVSTLAGEVENPQKTFPKALFVSVIFTCLSYLVPLLAVIGAVDVEQSAWGSGFHAQAAGI 300

Query: 301 IAGGKWLKILLEIGECLSAIGLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPWIG 360
           IA GKWLKILLEIG  LSAIGLFEAQLSSSAYQILGMAEIG+LPK    RAKWF TPWIG
Sbjct: 301 IA-GKWLKILLEIGSTLSAIGLFEAQLSSSAYQILGMAEIGVLPKFFGSRAKWFKTPWIG 360

Query: 361 IVICTAISLAVSYMNFDDIVATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPG 420
           IVICTAISLAVSYM+F DIVA+ANFIYSLGMLLE SSFVWLRWKHPG+KRPFKVPLKLPG
Sbjct: 361 IVICTAISLAVSYMDFTDIVASANFIYSLGMLLELSSFVWLRWKHPGIKRPFKVPLKLPG 420

Query: 421 LIVMCLVSSAFFVVVMYFTHKKVFLVSAAMTAAGVIWFELMKICKKKEIFEFNPRPEAIQ 480
           LIVMCL+ + F VVVM FTHK V LVS  MT  G++WF LMKICKKK+I EFNP PEAI 
Sbjct: 421 LIVMCLIPAGFLVVVMVFTHKNVLLVSVGMTVGGILWFGLMKICKKKKILEFNPEPEAIV 471

Query: 481 E 482
           E
Sbjct: 481 E 471

BLAST of Spg021830 vs. TAIR 10
Match: AT3G13620.1 (Amino acid permease family protein )

HSP 1 Score: 592.0 bits (1525), Expect = 4.2e-169
Identity = 288/456 (63.16%), Postives = 365/456 (80.04%), Query Frame = 0

Query: 19  ADSSSISQDLPTTTT-ATAPTAASNKLTLIPLIFIIYFQVSGGPYDEESAVQAAGPLLTI 78
           +++S  S +LP TT  ++   A + KLTLIPL+F+IYF+V+GGP+ EE AVQAAGPLL I
Sbjct: 4   SEASKSSHELPVTTAESSGKKATAKKLTLIPLVFLIYFEVAGGPFGEEPAVQAAGPLLAI 63

Query: 79  IGFLVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGPFWGSLIGTWKLLSGIINIT 138
           +GFL+FPFIWS+PEALITAELST FPG+GG+VIWA RAFG F GS++G+ K LSG+IN+ 
Sbjct: 64  LGFLIFPFIWSIPEALITAELSTAFPGNGGFVIWAHRAFGSFVGSMMGSLKFLSGVINVA 123

Query: 139 ACPVLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIGLTIVGYVAVVLAFLSLLPF 198
           + PVLCV Y+ K+   LESGWPR   I AST++LS LNY GL IVGY AVVL  +SL PF
Sbjct: 124 SFPVLCVTYLDKLFPVLESGWPRNVCIFASTVVLSFLNYTGLAIVGYAAVVLGLVSLSPF 183

Query: 199 MIMTFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYWDNVSTLAGEVQNPKKTFP 258
           ++M+ MAIP ++P RW SLG K  KKDWNLY NT+FWNLN+WDNVSTLAGEV  P+KTFP
Sbjct: 184 LVMSAMAIPKIKPHRWGSLGTK--KKDWNLYFNTLFWNLNFWDNVSTLAGEVDEPQKTFP 243

Query: 259 IALFVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAAETIAGGKWLKILLEIGECL 318
           +AL ++VIFTC++YLIP+ AV GAV V+QS WE+GFHA+AAE IA GKWLKI +EIG  L
Sbjct: 244 LALLIAVIFTCVAYLIPLFAVTGAVSVDQSRWENGFHAEAAEMIA-GKWLKIWIEIGAVL 303

Query: 319 SAIGLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPWIGIVICTAISLAVSYMNFD 378
           S+IGLFEAQLSSSAYQ+ GMAE+G LPK    R+KWFNTPW+GI+I   +SL +SYMNF 
Sbjct: 304 SSIGLFEAQLSSSAYQLEGMAELGFLPKFFGVRSKWFNTPWVGILISALMSLGLSYMNFT 363

Query: 379 DIVATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMCLVSSAFFVVVMY 438
           DI+++ANF+Y+LGM LEF+SF+WLR K P +KRP++VPLK+PGL+VMCL+ SAF V+++ 
Sbjct: 364 DIISSANFLYTLGMFLEFASFIWLRRKLPQLKRPYRVPLKIPGLVVMCLIPSAFLVLILV 423

Query: 439 FTHKKVFLVSAAMTAAGVIWFELMKICKKKEIFEFN 474
           F  K V+L+   MT   + W+ L+   +K +IFEFN
Sbjct: 424 FATKIVYLICGVMTIGAIGWYFLINYFRKTKIFEFN 456

BLAST of Spg021830 vs. TAIR 10
Match: AT5G05630.1 (Amino acid permease family protein )

HSP 1 Score: 408.3 bits (1048), Expect = 8.6e-114
Identity = 209/453 (46.14%), Postives = 305/453 (67.33%), Query Frame = 0

Query: 24  ISQDLPTTTTATAPTAASN---KLTLIPLIFIIYFQVSGGPYDEESAVQAAGPLLTIIGF 83
           IS  + T   AT+P    N   K+T++PL+F+I+++VSGGP+  E +V+AAGPLL I+GF
Sbjct: 30  ISIGVTTGDPATSPARTVNQIKKITVLPLVFLIFYEVSGGPFGIEDSVKAAGPLLAIVGF 89

Query: 84  LVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGPFWGSLIGTWKLLSGIINITACP 143
           +VFPFIWS+PEALITAE+ T FP +GGYV+W   A GP+WG   G  K LSG+I+    P
Sbjct: 90  IVFPFIWSIPEALITAEMGTMFPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYP 149

Query: 144 VLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIGLTIVGYVAVVLAFLSLLPFMIM 203
           +L ++Y+K     L SG PR  AIL  T+ L+ LNY GL+IVG  AV+L   S+LPF++M
Sbjct: 150 ILFLDYLKSGIPILGSGIPRVAAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVM 209

Query: 204 TFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYWDNVSTLAGEVQNPKKTFPIAL 263
           +FM+IP ++P RWL +  K    +W+LYLNT+FWNLNYWD+VSTL GEV+NP KT P AL
Sbjct: 210 SFMSIPKLKPSRWLVVSKKMKGVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRAL 269

Query: 264 FVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAAETIAGGKWLKILLEIGECLSAI 323
           F +++    SY+ P+L   GA+ ++Q  W  G+ A   + I GG WL   ++     S +
Sbjct: 270 FYALLLVVFSYIFPVLTGTGAIALDQKLWTDGYFADIGKVI-GGVWLGWWIQAAAATSNM 329

Query: 324 GLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPWIGIVICTAISLAVSYMNFDDIV 383
           G+F A++SS ++Q+LGMAE G+LP++ A R++ + TPW+GI+   +  + +S+++F +IV
Sbjct: 330 GMFLAEMSSDSFQLLGMAERGMLPEVFAKRSR-YRTPWVGILFSASGVIILSWLSFQEIV 389

Query: 384 ATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMCLVSSAFFVVVMYFTH 443
           A  N +Y  GM+LEF +FV LR K+P   RPFK+P+ + G ++MC+  +    V+M FT+
Sbjct: 390 AAENLLYCFGMVLEFITFVRLRMKYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTN 449

Query: 444 KKVFLVSAAMTAAGVIWFELMKICKKKEIFEFN 474
            KV LVS A    G++    +K  +KK   +F+
Sbjct: 450 LKVALVSLAAIVIGLVLQPCLKQVEKKGWLKFS 480

BLAST of Spg021830 vs. TAIR 10
Match: AT1G31830.2 (Amino acid permease family protein )

HSP 1 Score: 389.4 bits (999), Expect = 4.1e-108
Identity = 201/452 (44.47%), Postives = 298/452 (65.93%), Query Frame = 0

Query: 22  SSISQDLPTTTTATAPTAASNKLTLIPLIFIIYFQVSGGPYDEESAVQAAGPLLTIIGFL 81
           SS+  D   ++   A T    K++++PL+F+I+++VSGGP+  E +V AAGPLL ++GF+
Sbjct: 12  SSVGADEVPSSPPKA-TDKIRKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLLALLGFV 71

Query: 82  VFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGPFWGSLIGTWKLLSGIINITACPV 141
           +FPFIWS+PEALITAE+ T +P +GGYV+W   A GPFWG   G  K LSG+I+    PV
Sbjct: 72  IFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPV 131

Query: 142 LCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIGLTIVGYVAVVLAFLSLLPFMIMT 201
           L ++Y+K     L SG PR  +IL  T+LL+ LNY GLTIVG+VAV++   S+LPF +M 
Sbjct: 132 LFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMG 191

Query: 202 FMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYWDNVSTLAGEVQNPKKTFPIALF 261
            ++IP + P RWL + D G   +WNLYLNT+FWNLNYWD++STLAGEV+NP  T P ALF
Sbjct: 192 LISIPQLEPSRWLVM-DLG-NVNWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALF 251

Query: 262 VSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAAETIAGGKWLKILLEIGECLSAIG 321
             VI    SY+ P+LA +GA+ +E+  W  G+ +  A+ + GG WL+  ++     S +G
Sbjct: 252 YGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKAL-GGAWLRWWVQAAAATSNMG 311

Query: 322 LFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPWIGIVICTAISLAVSYMNFDDIVA 381
           +F A++SS ++Q+LGMAE G+LP+  A R++ + TP +GI+   +  + +S+++F +IVA
Sbjct: 312 MFIAEMSSDSFQLLGMAERGMLPEFFAKRSR-YGTPLLGILFSASGVVLLSWLSFQEIVA 371

Query: 382 TANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMCLVSSAFFVVVMYFTHK 441
             N +Y +GM+LEF +FV +R KHP   RP+K+P+   G I+MC+  +     V+  +  
Sbjct: 372 AENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALSSL 431

Query: 442 KVFLVSAAMTAAGVIWFELMKICKKKEIFEFN 474
           KV  VS  M   G +   L+    +K   +F+
Sbjct: 432 KVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFS 458

BLAST of Spg021830 vs. TAIR 10
Match: AT3G19553.1 (Amino acid permease family protein )

HSP 1 Score: 389.0 bits (998), Expect = 5.4e-108
Identity = 205/436 (47.02%), Postives = 288/436 (66.06%), Query Frame = 0

Query: 41  SNKLTLIPLIFIIYFQVSGGPYDEESAVQA-AGPLLTIIGFLVFPFIWSVPEALITAELS 100
           S KLTL+PL+F+I+++VSGGP+  E +V++  GPLL ++GFL+FP IWS+PEAL+TAEL+
Sbjct: 19  SPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWSIPEALVTAELA 78

Query: 101 TTFPGDGGYVIWAERAFGPFWGSLIGTWKLLSGIINITACPVLCVEYMKKIATPLESGWP 160
           T+FP +GGYV+W   AFGPFWG   G WK  SG+++    PVL ++Y+K     L+    
Sbjct: 79  TSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKHSFPVLDHVAA 138

Query: 161 RRTAILASTLLLSLLNYIGLTIVGYVAVVLAFLSLLPFMIMTFMAIPMVRPRRWLSLGDK 220
           R  A+L  T  L+ LNY GL IVG+ AVVLA  SL PF++M  +A+P +RP+RWL +  +
Sbjct: 139 RVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNIRPKRWLFVDTQ 198

Query: 221 GVKKDWNLYLNTMFWNLNYWDNVSTLAGEVQNPKKTFPIALFVSVIFTCLSYLIPILAVV 280
             K +W  Y NTMFWNLNYWD  STLAGEV  P KTFP ALF +V+    SYLIP++A  
Sbjct: 199 --KINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGSYLIPLMAGT 258

Query: 281 GAVDVEQS-TWESGFHAQAAETIAGGKWLKILLEIGECLSAIGLFEAQLSSSAYQILGMA 340
           GA+    S  W  G+ A+    I GG WLK  ++    +S +GLFEA++SS A+Q+LGM+
Sbjct: 259 GALSSSTSGEWSDGYFAEVGMLI-GGVWLKGWIQAAAAMSNLGLFEAEMSSDAFQLLGMS 318

Query: 341 EIGILPKLLAGRAKWFNTPWIGIVICTAISLAVSYMNFDDIVATANFIYSLGMLLEFSSF 400
           EIG+LP   A R+K + TP I I+      + +S+M+F +I+   NF+Y+LGMLLEF++F
Sbjct: 319 EIGMLPAFFAQRSK-YGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGMLLEFAAF 378

Query: 401 VWLRWKHPGMKRPFKVPLKLPGLIVMCLVSSAFFVVVMYFTHKKVFLVSAAMTAAGVIWF 460
           V LR K P + RP++VPL   G+ ++CL  S   ++VM     K FL+S  +   G   +
Sbjct: 379 VKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVIIVLGFCLY 438

Query: 461 ELMKICKKKEIFEFNP 475
             + + K+K+   F P
Sbjct: 439 PFLTLVKEKQWARFIP 450

BLAST of Spg021830 vs. TAIR 10
Match: AT1G31830.1 (Amino acid permease family protein )

HSP 1 Score: 387.1 bits (993), Expect = 2.1e-107
Identity = 201/466 (43.13%), Postives = 303/466 (65.02%), Query Frame = 0

Query: 8   LKCNHNHNQSTADSSSISQDLPTTTTATAPTAASNKLTLIPLIFIIYFQVSGGPYDEESA 67
           ++ +   N     SS  + ++P++      T    K++++PL+F+I+++VSGGP+  E +
Sbjct: 15  IEMSQYENNEVPYSSVGADEVPSSPPKA--TDKIRKVSMLPLVFLIFYEVSGGPFGVEDS 74

Query: 68  VQAAGPLLTIIGFLVFPFIWSVPEALITAELSTTFPGDGGYVIWAERAFGPFWGSLIGTW 127
           V AAGPLL ++GF++FPFIWS+PEALITAE+ T +P +GGYV+W   A GPFWG   G  
Sbjct: 75  VNAAGPLLALLGFVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWM 134

Query: 128 KLLSGIINITACPVLCVEYMKKIATPLESGWPRRTAILASTLLLSLLNYIGLTIVGYVAV 187
           K LSG+I+    PVL ++Y+K     L SG PR  +IL  T+LL+ LNY GLTIVG+VAV
Sbjct: 135 KWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAV 194

Query: 188 VLAFLSLLPFMIMTFMAIPMVRPRRWLSLGDKGVKKDWNLYLNTMFWNLNYWDNVSTLAG 247
           ++   S+LPF +M  ++IP + P RWL + D G   +WNLYLNT+FWNLNYWD++STLAG
Sbjct: 195 LMGVFSILPFAVMGLISIPQLEPSRWLVM-DLG-NVNWNLYLNTLFWNLNYWDSISTLAG 254

Query: 248 EVQNPKKTFPIALFVSVIFTCLSYLIPILAVVGAVDVEQSTWESGFHAQAAETIAGGKWL 307
           EV+NP  T P ALF  VI    SY+ P+LA +GA+ +E+  W  G+ +  A+ + GG WL
Sbjct: 255 EVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKAL-GGAWL 314

Query: 308 KILLEIGECLSAIGLFEAQLSSSAYQILGMAEIGILPKLLAGRAKWFNTPWIGIVICTAI 367
           +  ++     S +G+F A++SS ++Q+LGMAE G+LP+  A R++ + TP +GI+   + 
Sbjct: 315 RWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLPEFFAKRSR-YGTPLLGILFSASG 374

Query: 368 SLAVSYMNFDDIVATANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMCLV 427
            + +S+++F +IVA  N +Y +GM+LEF +FV +R KHP   RP+K+P+   G I+MC+ 
Sbjct: 375 VVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIP 434

Query: 428 SSAFFVVVMYFTHKKVFLVSAAMTAAGVIWFELMKICKKKEIFEFN 474
            +     V+  +  KV  VS  M   G +   L+    +K   +F+
Sbjct: 435 PTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFS 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878316.13.2e-21982.16probable polyamine transporter At3g13620 [Benincasa hispida][more]
KAG6588239.12.1e-21879.71putative polyamine transporter, partial [Cucurbita argyrosperma subsp. sororia] ... [more]
XP_022933749.11.3e-21779.50probable polyamine transporter At3g13620 [Cucurbita moschata][more]
XP_023006107.12.3e-21779.55probable polyamine transporter At3g13620 [Cucurbita maxima][more]
XP_023529220.18.6e-21779.09probable polyamine transporter At3g13620 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LHN75.9e-16863.16Probable polyamine transporter At3g13620 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
Q9FFL11.2e-11246.14Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1[more]
A2X8M86.9e-10846.22Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 S... [more]
Q6Z8D06.9e-10846.22Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1... [more]
Q9LH397.6e-10747.02Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
Match NameE-valueIdentityDescription
A0A6J1F0M16.5e-21879.50probable polyamine transporter At3g13620 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1KV061.1e-21779.55probable polyamine transporter At3g13620 OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
A0A0A0M3002.2e-21078.17Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G630310 PE=3 SV=1[more]
A0A5A7UQT66.5e-21078.17Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3BNB66.5e-21078.17probable polyamine transporter At3g13620 OS=Cucumis melo OX=3656 GN=LOC103491953... [more]
Match NameE-valueIdentityDescription
AT3G13620.14.2e-16963.16Amino acid permease family protein [more]
AT5G05630.18.6e-11446.14Amino acid permease family protein [more]
AT1G31830.24.1e-10844.47Amino acid permease family protein [more]
AT3G19553.15.4e-10847.02Amino acid permease family protein [more]
AT1G31830.12.1e-10743.13Amino acid permease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 67..441
e-value: 1.9E-32
score: 112.7
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 43..474
e-value: 3.4E-57
score: 196.0
NoneNo IPR availablePIRSFPIRSF006060AA_transportercoord: 27..475
e-value: 8.3E-60
score: 199.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..34
NoneNo IPR availablePANTHERPTHR45826:SF8BNAC05G39680D PROTEINcoord: 18..475
IPR044566Polyamine transporter RMV1-likePANTHERPTHR45826POLYAMINE TRANSPORTER PUT1coord: 18..475

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg021830.1Spg021830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902047 polyamine transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015203 polyamine transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity