Spg021806 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg021806 vs. NCBI nr
Match: XP_038880237.1 (uncharacterized vacuolar membrane protein YML018C-like [Benincasa hispida])

HSP 1 Score: 689.1 bits (1777), Expect = 2.4e-194
Identity = 367/427 (85.95%), Postives = 389/427 (91.10%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYK GLILL+AVVVIWV+SAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  MKLYRSHFRKGNSKKVAEIQP--PAIEERTSQSSSIEED--NNNNNNNIISEVENGDVHN 120
           +K++RSHFR GNS+KVAE+QP  PA+EERT+QSS  ++D  NNNNNNNIISEV NGDV  
Sbjct: 61  VKIFRSHFRNGNSRKVAELQPPLPALEERTNQSSIEDDDNNNNNNNNNIISEVGNGDV-Q 120

Query: 121 CVVNIECEEEEEVRVNK---NGGCECEGENGGMRLKTEEITKFCTKQIAVLALAIGPIWF 180
           CVVNI   + E++RVN    NGGCECE EN  M +   E TK  TKQIAVLAL IGPIWF
Sbjct: 121 CVVNI--IQGEDLRVNNNNHNGGCECEPENLEMTILKTEETKCSTKQIAVLALVIGPIWF 180

Query: 181 VSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTT 240
           VSEYFTNAALARTSVATTTILFSTSGLFTLIIDAC+ERQSLTIVNVVAV VSMAGVAMTT
Sbjct: 181 VSEYFTNAALARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNVVAVIVSMAGVAMTT 240

Query: 241 VGKTWARDEAQSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLG 300
           VGKTWARDE +SSSS HGKHSYVGD+FALLSA TDGLYYVLLKKYAGEEGEKVDMQKFLG
Sbjct: 241 VGKTWARDEVRSSSSEHGKHSYVGDIFALLSALTDGLYYVLLKKYAGEEGEKVDMQKFLG 300

Query: 301 YVGLFTLTALWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGVVWT 360
           YVGLFTLT LWWL+WPLRAIGIEPKF IPQSTKVAE+VLANCFV+NFVSDYFWAMGVVWT
Sbjct: 301 YVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAELVLANCFVSNFVSDYFWAMGVVWT 360

Query: 361 SPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLKLRK 420
           SPLVAALGASLTIPLAMLGDMVLHGRHYSL+YI GSVQVFLGFMIANLSDWISPKLKLRK
Sbjct: 361 SPLVAALGASLTIPLAMLGDMVLHGRHYSLIYIFGSVQVFLGFMIANLSDWISPKLKLRK 420

BLAST of Spg021806 vs. NCBI nr
Match: XP_023531720.1 (uncharacterized transporter C405.03c-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 673.3 bits (1736), Expect = 1.3e-189
Identity = 355/420 (84.52%), Postives = 382/420 (90.95%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYKGGLILL+AVVVIWV+SAEITQSIFTDYEHPFV+TYVGTSMLVAYLAIAFI+ECI
Sbjct: 1   MAWKYKGGLILLVAVVVIWVASAEITQSIFTDYEHPFVMTYVGTSMLVAYLAIAFIRECI 60

Query: 61  MKLYRSHFRKGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNIISEVENGDVHNCVVN 120
           +KL+RSHF   NSK+VAEIQ    E+  + +++   +NNNNNNN ISEVENGDVH CVVN
Sbjct: 61  VKLFRSHFPNRNSKEVAEIQ----EQDNNNNNNNTNNNNNNNNNNISEVENGDVH-CVVN 120

Query: 121 IECEEEEEVRVNKNGGCECEGENGGMRLKTEEITKFCTKQIAVLALAIGPIWFVSEYFTN 180
           IECEE+E+  V+ NG CECE ENG     T+E T F TKQ+AVLALAIGPIWFVSEYFTN
Sbjct: 121 IECEEQEKGSVSNNGRCECEAENG-----TQE-TPFSTKQMAVLALAIGPIWFVSEYFTN 180

Query: 181 AALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTTVGKTWAR 240
           AALARTSVATTTILFSTSGLFTLI+DAC ERQSL+IVNVVAVFVSMAGVAMTTVGKTWAR
Sbjct: 181 AALARTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSMAGVAMTTVGKTWAR 240

Query: 241 DEAQSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL 300
           DE Q SS GH KHSY+GDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL
Sbjct: 241 DETQ-SSFGHEKHSYIGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL 300

Query: 301 TALWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGVVWTSPLVAAL 360
           T LWWL+WPLRA+GIEPKF IPQSTKVAEIVLANCFV+NFVSDYFWAMGVVWTSPLVAAL
Sbjct: 301 TTLWWLIWPLRAMGIEPKFGIPQSTKVAEIVLANCFVSNFVSDYFWAMGVVWTSPLVAAL 360

Query: 361 GASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLKLRKKFFNGTK 420
           GASLTIPLAM+GDM+LHGRHYSLVYI GS+QVFLGFMIAN SDWISPKLKLRKKFFNG K
Sbjct: 361 GASLTIPLAMVGDMLLHGRHYSLVYIFGSLQVFLGFMIANFSDWISPKLKLRKKFFNGAK 408

BLAST of Spg021806 vs. NCBI nr
Match: XP_022925420.1 (uncharacterized transporter C405.03c-like [Cucurbita moschata])

HSP 1 Score: 667.9 bits (1722), Expect = 5.6e-188
Identity = 350/420 (83.33%), Postives = 377/420 (89.76%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYKGGLILL+AVVVIWV+SAEITQSIFTDYEHPFV+TYVGTSMLVAYLAIAF++ECI
Sbjct: 1   MAWKYKGGLILLVAVVVIWVASAEITQSIFTDYEHPFVMTYVGTSMLVAYLAIAFVRECI 60

Query: 61  MKLYRSHFRKGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNIISEVENGDVHNCVVN 120
           +KL+RSHF  GN K+VAEIQ         +  +   +NNNNNNN ISEVENGDVH CVVN
Sbjct: 61  VKLFRSHFPNGNPKEVAEIQ---------EQDNNNNNNNNNNNNNISEVENGDVH-CVVN 120

Query: 121 IECEEEEEVRVNKNGGCECEGENGGMRLKTEEITKFCTKQIAVLALAIGPIWFVSEYFTN 180
           +ECEE+E+   + NG CECE ENG     T+E T F TKQ+AVLALAIGPIWFVSEYFTN
Sbjct: 121 VECEEQEKGSASNNGRCECEAENG-----TQE-TSFSTKQMAVLALAIGPIWFVSEYFTN 180

Query: 181 AALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTTVGKTWAR 240
           AALA+TSVATTTILFSTSGLFTLI+DAC ERQSL+IVNVVAVFVSMAGVAMTTVGKTWAR
Sbjct: 181 AALAKTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSMAGVAMTTVGKTWAR 240

Query: 241 DEAQSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL 300
           DEAQ SS GH KHSY+GDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL
Sbjct: 241 DEAQ-SSFGHEKHSYIGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL 300

Query: 301 TALWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGVVWTSPLVAAL 360
           T LWWL+WPLRAIGIEPKF +PQSTKVAEIVLANCFV+NFVSDY WAMGVVWTSPLVAAL
Sbjct: 301 TTLWWLIWPLRAIGIEPKFGLPQSTKVAEIVLANCFVSNFVSDYLWAMGVVWTSPLVAAL 360

Query: 361 GASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLKLRKKFFNGTK 420
           GASLTIPLAM+GDM+LHGRHYSLVYI GS+QVFLGFMIAN SDWISPKLKLRKKFFNG K
Sbjct: 361 GASLTIPLAMVGDMLLHGRHYSLVYIFGSLQVFLGFMIANFSDWISPKLKLRKKFFNGAK 403

BLAST of Spg021806 vs. NCBI nr
Match: XP_023007421.1 (uncharacterized vacuolar membrane protein YML018C-like [Cucurbita maxima])

HSP 1 Score: 661.4 bits (1705), Expect = 5.3e-186
Identity = 348/420 (82.86%), Postives = 382/420 (90.95%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYKGGLILL+ VVVIWV+SAEITQSIF DYEHPFV+TYVGTSMLVAYLAIAF++EC+
Sbjct: 1   MAWKYKGGLILLVVVVVIWVASAEITQSIFMDYEHPFVMTYVGTSMLVAYLAIAFLRECL 60

Query: 61  MKLYRSHFRKGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNIISEVENGDVHNCVVN 120
           +KL+RSHFR GNSK+VAEIQ    +++ + +++I  +NNNNNNN ISEVENGDV  CVVN
Sbjct: 61  VKLFRSHFRNGNSKEVAEIQE---QDKDNNNNNINNNNNNNNNN-ISEVENGDV-LCVVN 120

Query: 121 IECEEEEEVRVNKNGGCECEGENGGMRLKTEEITKFCTKQIAVLALAIGPIWFVSEYFTN 180
           IECEE+E+  V+ N  CECE ENG     T+E T F TK+IAVLALAIGPIWFVSEYFTN
Sbjct: 121 IECEEQEKGSVSNNDRCECEAENG-----TQE-TSFSTKKIAVLALAIGPIWFVSEYFTN 180

Query: 181 AALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTTVGKTWAR 240
           AALARTSVATTTILFSTSGLFTLI+DAC ERQSL+IVNVVAVFVSMAGVAMTT+GKTWAR
Sbjct: 181 AALARTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSMAGVAMTTIGKTWAR 240

Query: 241 DEAQSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL 300
           DEAQSSS  H KHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL
Sbjct: 241 DEAQSSSR-HEKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL 300

Query: 301 TALWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGVVWTSPLVAAL 360
           T LWWL+WPL+AIGIEPKF IPQSTKVAEIV+ANCFV+NFVSDY WAMGVVWTSPL+AAL
Sbjct: 301 TTLWWLIWPLKAIGIEPKFGIPQSTKVAEIVIANCFVSNFVSDYLWAMGVVWTSPLIAAL 360

Query: 361 GASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLKLRKKFFNGTK 420
           GASLTIPLAM+GDM+LHGRHYSLVYI GS+QVFLGFMIAN SDWISPKLKLRKKFF+G K
Sbjct: 361 GASLTIPLAMVGDMLLHGRHYSLVYIFGSLQVFLGFMIANFSDWISPKLKLRKKFFDGAK 408

BLAST of Spg021806 vs. NCBI nr
Match: TYK06345.1 (putative transporter-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 648.7 bits (1672), Expect = 3.5e-182
Identity = 349/424 (82.31%), Postives = 367/424 (86.56%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYK GLILL+AVVVIWV+SAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVTSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  MKLYRSHFR-KGNSKKVAEIQP--PAIEERTSQSSSIEEDNNNNNNNIISEVENGDVHNC 120
           MK+ RSHFR KGN +KVAEIQ   PA+EERT+QSS      NNNNN    +VEN DV   
Sbjct: 61  MKIVRSHFRTKGNCRKVAEIQSPLPALEERTNQSS------NNNNNTTSDKVENRDVQYV 120

Query: 121 VVNIECEEEEEVRVNKNGGCEC-EGENGGMRLKTEEITKFCTKQIAVLALAIGPIWFVSE 180
           V   +         N NGGCE  E EN  MR+   E  KF TKQIAVLAL IGPIWFVSE
Sbjct: 121 VNINDNNNNSNNSSNNNGGCELREAENMEMRILKTEEAKFSTKQIAVLALTIGPIWFVSE 180

Query: 181 YFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTTVGK 240
           YFTNAALARTSVATTTILFSTSGLFTLI+DAC+ERQSLTIVNVVAV VSMAGVAMTTVGK
Sbjct: 181 YFTNAALARTSVATTTILFSTSGLFTLILDACLERQSLTIVNVVAVVVSMAGVAMTTVGK 240

Query: 241 TWARDEAQSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVG 300
           TWA+DE QSSS GHGKHS+VGD FALLSA TDGLYYVLLKKYAGEEGEKVDMQKFLGYVG
Sbjct: 241 TWAKDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKVDMQKFLGYVG 300

Query: 301 LFTLTALWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGVVWTSPL 360
           LFTLT LWWL+WPLRAIGIEPKF IPQSTKVAE+VLANCFVANFVSDYFWAMGVVWTSPL
Sbjct: 301 LFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFVANFVSDYFWAMGVVWTSPL 360

Query: 361 VAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLKLRKKFF 420
           VAALGASLTIPLAM+GDMVLHGRHYSLVYI GS+QVFLGF+IAN SDWISPKLKLRKKFF
Sbjct: 361 VAALGASLTIPLAMIGDMVLHGRHYSLVYIFGSIQVFLGFIIANFSDWISPKLKLRKKFF 418

BLAST of Spg021806 vs. ExPASy Swiss-Prot
Match: Q03730 (Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YML018C PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.8e-33
Identity = 119/401 (29.68%), Postives = 193/401 (48.13%), Query Frame = 0

Query: 8   GLILLIAVVVIWVSSAEITQSIFTD--YEHPFVVTYVGTSMLVAYLAIAFIKECIMKLYR 67
           GL++L  V+++WV S+ +   IF D  Y  PF +TY  T+  + YL      + ++  Y+
Sbjct: 16  GLLMLGLVIILWVLSSFLINLIFEDDSYRKPFFITYTNTAAFIFYLFPT--AKAVVVNYK 75

Query: 68  SHFRKGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNIISEVENGDVHNCVVNIECEE 127
              R    +++                 +EE+   +++N   ++ +  + N        +
Sbjct: 76  DTGRANVHRELI----------------MEEEGTGSDSNRSVDMTSPLLTNLEAGTHANQ 135

Query: 128 EEEVRVNKNGGCECEGENGGMRLKTEEITKFCTKQIAVLALAIGPIWFVSEYFTNAALAR 187
           ++ + + +            ++L  E    FC             +WF +   TNA+LA 
Sbjct: 136 KKRLTLYET-----------IKLSAE----FCI------------LWFTANLVTNASLAF 195

Query: 188 TSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTTVGKTWARDEAQ- 247
           TSVA+ TIL +TS  FTL I A    +SL+   V+  F+S  G+ M T   +  R +   
Sbjct: 196 TSVASQTILSTTSSFFTLFIGAICHVESLSKSKVLGSFISFVGIIMVTKSDSHQRYQRHI 255

Query: 248 ---SSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTLT 307
              S          +G++ AL  A   G+Y  LLK+  G+E  +V+M+ F G+VGLF L 
Sbjct: 256 ADVSGDDNDAVQVLIGNLLALAGAVLYGVYSTLLKREVGDE-TRVNMKIFFGFVGLFNLL 315

Query: 308 ALWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGVVWTSPLVAALG 367
            LW  +  L   G EP F++P+  KV  I+  NC +  FVSD+ WA  ++ TSPL   +G
Sbjct: 316 FLWPSLIVLDFFGWEP-FSLPKDPKVVVIIFVNCLI-TFVSDFCWAKAMLLTSPLTVTVG 368

Query: 368 ASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLS 403
            S+TIPLAM GD++   +  S +Y+ G+  +   F I N S
Sbjct: 376 LSITIPLAMFGDVIFKHKTMSALYLFGATLILGSFFIINKS 368

BLAST of Spg021806 vs. ExPASy Swiss-Prot
Match: O94654 (Uncharacterized transporter C405.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC405.03c PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 3.5e-31
Identity = 118/399 (29.57%), Postives = 185/399 (46.37%), Query Frame = 0

Query: 4   KYKGGLILLIAVVVIWVSSAEITQSIFTD--YEHPFVVTYVGTSMLVAYLAIAFIKECIM 63
           K+  G++LL+ VV +W+ S+ +T S+  D  +  PF++TY+ T   V YL   +  E   
Sbjct: 8   KHALGVVLLLFVVFLWLISSFLTSSLLDDDNFFSPFLITYINTGTFVFYLIPWYFSE--- 67

Query: 64  KLYRSHFRKGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNIISEVENGDVHNCVVNI 123
           K  R H                   R     S+ E                 VH+   N 
Sbjct: 68  KKTRKH-------------------RLMSELSMYE----------------SVHDSSFN- 127

Query: 124 ECEEEEEVRVNKNGGCECEGENGGMRLKTEEITKFCTKQIAVLALAIGPIWFVSEYFTNA 183
                                     L T   +    +Q A L+L    IWF + YF+N+
Sbjct: 128 --------------------------LGTRPNSPLGFRQTAYLSLGFCIIWFAANYFSNS 187

Query: 184 ALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTTVGKTWARD 243
           +L  T+VA+ TI+ S SG FTL +   +  +  T+  ++A+  S+ GV +         D
Sbjct: 188 SLGFTNVASFTIISSMSGFFTLGLGTIVNVERFTLSKLLALMASVGGVIIVVTQDAKQAD 247

Query: 244 EAQSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTLT 303
              S  S       +G+ +ALL+A   G Y V++K +  EE   V  + F G VGLF L 
Sbjct: 248 LNDSPPS----RPALGNAYALLAALLYGCYSVMVKFHITEE-SCVSTRLFFGLVGLFDLI 307

Query: 304 ALWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGVVWTSPLVAALG 363
            LW  +  L   G+E +F++P +T    +++ N  +  FVSDY W + ++ TSPL+  +G
Sbjct: 308 LLWPFLIILHLYGVE-RFSLPSTTAGLIVLIINASI-TFVSDYLWVIAMLMTSPLLVTVG 334

Query: 364 ASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIAN 401
            SL+IPLA+  D++L G + +   ILGS+ VF GF++ N
Sbjct: 368 MSLSIPLALFFDILLKGHYLNFSLILGSLLVFAGFIVVN 334

BLAST of Spg021806 vs. ExPASy Swiss-Prot
Match: Q04083 (Thiamine-repressible mitochondrial transport protein THI74 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=THI74 PE=1 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 6.5e-30
Identity = 109/395 (27.59%), Postives = 181/395 (45.82%), Query Frame = 0

Query: 8   GLILLIAVVVIWVSSAEITQSIF--TDYEHPFVVTYVGTSMLVAYLAIAFIKECIMKLYR 67
           G++LL  VVV WV ++ +T  +     Y  PF +TY+  S    YL        + ++ +
Sbjct: 13  GVLLLAVVVVFWVGASCLTNELLETNAYNKPFFLTYLNISSFALYLT-----PDLWRIIQ 72

Query: 68  SHFRKGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNIISEVENGDVHNCVVNIECEE 127
           S  RK   ++     P   +E  S+   +     + ++N+ S                  
Sbjct: 73  SR-RKSLQERTERTLPIHTQESFSEFLPLLSSTPSTSSNLSS------------------ 132

Query: 128 EEEVRVNKNGGCECEGENGGMRLKTEEITKFCTKQIAVLALAIGPIWFVSEYFTNAALAR 187
                                      I     K    L+L    +WFV+    NAAL+ 
Sbjct: 133 ---------------------------IADTKVKDTMRLSLLFCVLWFVANLAANAALSY 192

Query: 188 TSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTTVGKTWARDEAQS 247
           T+VA++TIL STS  FTL +   +  ++ +   ++ +FVS+ G+ +  +  +  +D   +
Sbjct: 193 TTVASSTILSSTSSFFTLFLATSLGIETFSTKKLLGLFVSLFGIILIVMQSSKQQDSVSA 252

Query: 248 SSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTLTALWW 307
           SS        VG+  ALL +    +Y  LLK     +G ++D+Q FLGYVG+FT    W 
Sbjct: 253 SS------FLVGNTLALLGSLGYSVYTTLLKYEISSKGLRLDIQMFLGYVGIFTFLLFWP 312

Query: 308 LVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGVVWTSPLVAALGASLT 367
           ++  L    +E  F +P +  ++ +V+ NC +  FVSDYFW   ++ TSPLV  +  + T
Sbjct: 313 ILIILDITHME-TFELPSNFHISFLVMLNCIII-FVSDYFWCKALILTSPLVVTVALTFT 348

Query: 368 IPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIAN 401
           IPLAM  D V     ++  YI+G + +F+ F + N
Sbjct: 373 IPLAMFADFVWREAFFTPWYIIGVIFIFVSFFLVN 348

BLAST of Spg021806 vs. ExPASy Swiss-Prot
Match: A6QL92 (Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 5.5e-29
Identity = 125/437 (28.60%), Postives = 204/437 (46.68%), Query Frame = 0

Query: 8   GLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECIMKLYRSH 67
           G+++L+ V VIWV+S+E+T  +FT Y  PF  T+  TSM V YL + FI   + K +R  
Sbjct: 72  GIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYL-LGFI---VWKPWRQQ 131

Query: 68  FRKGNSKK------VAEIQPPAIEERTSQSSSIEE----------------DNNNNNNNI 127
             +G   K       AE    A    T+ +SS+ E                +N N +   
Sbjct: 132 CTRGFRGKHATFFADAEGYFAACTTDTTMNSSLSEPLYVPVKFHDLPSEKPENTNIDTEK 191

Query: 128 ISEVENGDVHNCVVNIECEEEEEVRVNKNGGCECEGENGGMRLKTEEITKFCTKQIAVLA 187
           I +       N +   +      +    +       +     LKT  + K    Q+A ++
Sbjct: 192 IPKKSRVRFSNIMEIRQLPSSHALEAKLSRMSYPTVKEQESLLKT--VGKLTATQVAKIS 251

Query: 188 LAIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQS---LTIVNVVAV 247
                +WF++ +    AL+ T VA   IL STSGLFTLI+ A     S    T+  ++AV
Sbjct: 252 FFFCFVWFLANFSYQEALSDTQVAIVNILSSTSGLFTLILAAMFPSNSGDRFTLSKLLAV 311

Query: 248 FVSMAGVAMTTVGKTWARDEAQSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEE 307
            +S+ GV +  +           S    G+++ +G +++L+ A    +Y V++K+    E
Sbjct: 312 ILSIGGVVLVNL---------SGSEKSPGRNT-IGSIWSLVGAMLYAVYIVMIKRKVDRE 371

Query: 308 GEKVDMQKFLGYVGLFTLTALWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVS 367
            +K+D+  F G+VGLF L  LW   + L   G E  F  P    +  IV+ N  +   +S
Sbjct: 372 -DKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEFPNKVVLMCIVI-NGLIGTVLS 431

Query: 368 DYFWAMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANL- 416
           ++ W  G   TS L+  L  SLTIPL+++ DM +    +S ++  G++ VF  F IA L 
Sbjct: 432 EFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGAIPVFFSFFIATLL 489

BLAST of Spg021806 vs. ExPASy Swiss-Prot
Match: Q5R6J3 (Solute carrier family 35 member F5 OS=Pongo abelii OX=9601 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.7e-28
Identity = 127/438 (29.00%), Postives = 199/438 (45.43%), Query Frame = 0

Query: 8   GLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECIMKLYRSH 67
           G+++L+ V VIWV+S+E+T  +FT Y  PF  T+  TSM V YL + FI   I K +R  
Sbjct: 72  GIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYL-LGFI---IWKPWRQQ 131

Query: 68  FRKGNSKK------VAEIQPPAIEERTSQSSSIEE------------DNNNNNNNIISEV 127
             +G   K       AE    A    T+ +SS+ E                 + NI +E 
Sbjct: 132 CTRGLRGKHAAFFADAEGYFAACATDTTMNSSLSEPLYVPVKFHDLPSEKPESTNIDTEK 191

Query: 128 ENGDVHNCVVNIECEEEEEVRVNKNGGCECEGENGGMRLKTEE-----ITKFCTKQIAVL 187
                     NI     E  ++  N   E +       +K +E     + K    Q+A +
Sbjct: 192 TPKKSRVRFSNI----MEIRQLPSNHALESKLSRMSYPVKEQESILKTVGKLTATQVAKI 251

Query: 188 ALAIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQS---LTIVNVVA 247
           +     +WF++      AL+ T VA   IL STSGLFTLI+ A     S    T+  ++A
Sbjct: 252 SFFFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNSGDRFTLSKLLA 311

Query: 248 VFVSMAGVAMTTVGKTWARDEAQSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGE 307
           V +S+ GV +  +          S S        +G +++L  A    +Y V++K+    
Sbjct: 312 VILSIGGVVLVNL----------SGSEKSAGRDTIGSIWSLAGAMLYAVYIVMIKRKVDR 371

Query: 308 EGEKVDMQKFLGYVGLFTLTALWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFV 367
           E +K+D+  F G+VGLF L  LW   + L   G E  F  P    V   ++ N  +   +
Sbjct: 372 E-DKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEFPNKV-VLMCIIINGLIGTVL 431

Query: 368 SDYFWAMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANL 416
           S++ W  G   TS L+  L  SLTIPL+++ DM +    +S ++  G++ VF  F I  L
Sbjct: 432 SEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGAIPVFFSFFIVTL 488

BLAST of Spg021806 vs. ExPASy TrEMBL
Match: A0A6J1EHX2 (uncharacterized transporter C405.03c-like OS=Cucurbita moschata OX=3662 GN=LOC111432715 PE=4 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.7e-188
Identity = 350/420 (83.33%), Postives = 377/420 (89.76%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYKGGLILL+AVVVIWV+SAEITQSIFTDYEHPFV+TYVGTSMLVAYLAIAF++ECI
Sbjct: 1   MAWKYKGGLILLVAVVVIWVASAEITQSIFTDYEHPFVMTYVGTSMLVAYLAIAFVRECI 60

Query: 61  MKLYRSHFRKGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNIISEVENGDVHNCVVN 120
           +KL+RSHF  GN K+VAEIQ         +  +   +NNNNNNN ISEVENGDVH CVVN
Sbjct: 61  VKLFRSHFPNGNPKEVAEIQ---------EQDNNNNNNNNNNNNNISEVENGDVH-CVVN 120

Query: 121 IECEEEEEVRVNKNGGCECEGENGGMRLKTEEITKFCTKQIAVLALAIGPIWFVSEYFTN 180
           +ECEE+E+   + NG CECE ENG     T+E T F TKQ+AVLALAIGPIWFVSEYFTN
Sbjct: 121 VECEEQEKGSASNNGRCECEAENG-----TQE-TSFSTKQMAVLALAIGPIWFVSEYFTN 180

Query: 181 AALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTTVGKTWAR 240
           AALA+TSVATTTILFSTSGLFTLI+DAC ERQSL+IVNVVAVFVSMAGVAMTTVGKTWAR
Sbjct: 181 AALAKTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSMAGVAMTTVGKTWAR 240

Query: 241 DEAQSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL 300
           DEAQ SS GH KHSY+GDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL
Sbjct: 241 DEAQ-SSFGHEKHSYIGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL 300

Query: 301 TALWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGVVWTSPLVAAL 360
           T LWWL+WPLRAIGIEPKF +PQSTKVAEIVLANCFV+NFVSDY WAMGVVWTSPLVAAL
Sbjct: 301 TTLWWLIWPLRAIGIEPKFGLPQSTKVAEIVLANCFVSNFVSDYLWAMGVVWTSPLVAAL 360

Query: 361 GASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLKLRKKFFNGTK 420
           GASLTIPLAM+GDM+LHGRHYSLVYI GS+QVFLGFMIAN SDWISPKLKLRKKFFNG K
Sbjct: 361 GASLTIPLAMVGDMLLHGRHYSLVYIFGSLQVFLGFMIANFSDWISPKLKLRKKFFNGAK 403

BLAST of Spg021806 vs. ExPASy TrEMBL
Match: A0A6J1L0H0 (uncharacterized vacuolar membrane protein YML018C-like OS=Cucurbita maxima OX=3661 GN=LOC111499921 PE=4 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.6e-186
Identity = 348/420 (82.86%), Postives = 382/420 (90.95%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYKGGLILL+ VVVIWV+SAEITQSIF DYEHPFV+TYVGTSMLVAYLAIAF++EC+
Sbjct: 1   MAWKYKGGLILLVVVVVIWVASAEITQSIFMDYEHPFVMTYVGTSMLVAYLAIAFLRECL 60

Query: 61  MKLYRSHFRKGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNIISEVENGDVHNCVVN 120
           +KL+RSHFR GNSK+VAEIQ    +++ + +++I  +NNNNNNN ISEVENGDV  CVVN
Sbjct: 61  VKLFRSHFRNGNSKEVAEIQE---QDKDNNNNNINNNNNNNNNN-ISEVENGDV-LCVVN 120

Query: 121 IECEEEEEVRVNKNGGCECEGENGGMRLKTEEITKFCTKQIAVLALAIGPIWFVSEYFTN 180
           IECEE+E+  V+ N  CECE ENG     T+E T F TK+IAVLALAIGPIWFVSEYFTN
Sbjct: 121 IECEEQEKGSVSNNDRCECEAENG-----TQE-TSFSTKKIAVLALAIGPIWFVSEYFTN 180

Query: 181 AALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTTVGKTWAR 240
           AALARTSVATTTILFSTSGLFTLI+DAC ERQSL+IVNVVAVFVSMAGVAMTT+GKTWAR
Sbjct: 181 AALARTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSMAGVAMTTIGKTWAR 240

Query: 241 DEAQSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL 300
           DEAQSSS  H KHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL
Sbjct: 241 DEAQSSSR-HEKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL 300

Query: 301 TALWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGVVWTSPLVAAL 360
           T LWWL+WPL+AIGIEPKF IPQSTKVAEIV+ANCFV+NFVSDY WAMGVVWTSPL+AAL
Sbjct: 301 TTLWWLIWPLKAIGIEPKFGIPQSTKVAEIVIANCFVSNFVSDYLWAMGVVWTSPLIAAL 360

Query: 361 GASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLKLRKKFFNGTK 420
           GASLTIPLAM+GDM+LHGRHYSLVYI GS+QVFLGFMIAN SDWISPKLKLRKKFF+G K
Sbjct: 361 GASLTIPLAMVGDMLLHGRHYSLVYIFGSLQVFLGFMIANFSDWISPKLKLRKKFFDGAK 408

BLAST of Spg021806 vs. ExPASy TrEMBL
Match: A0A5D3C4N7 (Putative transporter-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00180 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.7e-182
Identity = 349/424 (82.31%), Postives = 367/424 (86.56%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYK GLILL+AVVVIWV+SAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVTSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  MKLYRSHFR-KGNSKKVAEIQP--PAIEERTSQSSSIEEDNNNNNNNIISEVENGDVHNC 120
           MK+ RSHFR KGN +KVAEIQ   PA+EERT+QSS      NNNNN    +VEN DV   
Sbjct: 61  MKIVRSHFRTKGNCRKVAEIQSPLPALEERTNQSS------NNNNNTTSDKVENRDVQYV 120

Query: 121 VVNIECEEEEEVRVNKNGGCEC-EGENGGMRLKTEEITKFCTKQIAVLALAIGPIWFVSE 180
           V   +         N NGGCE  E EN  MR+   E  KF TKQIAVLAL IGPIWFVSE
Sbjct: 121 VNINDNNNNSNNSSNNNGGCELREAENMEMRILKTEEAKFSTKQIAVLALTIGPIWFVSE 180

Query: 181 YFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTTVGK 240
           YFTNAALARTSVATTTILFSTSGLFTLI+DAC+ERQSLTIVNVVAV VSMAGVAMTTVGK
Sbjct: 181 YFTNAALARTSVATTTILFSTSGLFTLILDACLERQSLTIVNVVAVVVSMAGVAMTTVGK 240

Query: 241 TWARDEAQSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVG 300
           TWA+DE QSSS GHGKHS+VGD FALLSA TDGLYYVLLKKYAGEEGEKVDMQKFLGYVG
Sbjct: 241 TWAKDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKVDMQKFLGYVG 300

Query: 301 LFTLTALWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGVVWTSPL 360
           LFTLT LWWL+WPLRAIGIEPKF IPQSTKVAE+VLANCFVANFVSDYFWAMGVVWTSPL
Sbjct: 301 LFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFVANFVSDYFWAMGVVWTSPL 360

Query: 361 VAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLKLRKKFF 420
           VAALGASLTIPLAM+GDMVLHGRHYSLVYI GS+QVFLGF+IAN SDWISPKLKLRKKFF
Sbjct: 361 VAALGASLTIPLAMIGDMVLHGRHYSLVYIFGSIQVFLGFIIANFSDWISPKLKLRKKFF 418

BLAST of Spg021806 vs. ExPASy TrEMBL
Match: A0A5A7U493 (Putative transporter-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00790 PE=4 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 2.2e-182
Identity = 348/424 (82.08%), Postives = 365/424 (86.08%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYK GLILL+AVVVIWV+SAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVTSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  MKLYRSHFR-KGNSKKVAEIQP--PAIEERTSQSSSIEEDNNNNNNNIISEVENGDVHNC 120
           MK+ RSHFR KGN +KVAEIQ   PA+EERT+QSS      NNNNN    EVEN DV   
Sbjct: 61  MKIVRSHFRTKGNCRKVAEIQSPLPALEERTNQSS------NNNNNTTSDEVENRDVQYV 120

Query: 121 VVNIECEEEEEVRVNKNGGCE-CEGENGGMRLKTEEITKFCTKQIAVLALAIGPIWFVSE 180
           V   +         N NGGCE CE EN  MR+   E  KF TKQIAVLAL IGPIWFVSE
Sbjct: 121 VNINDNNNNSNNSSNNNGGCELCEAENMEMRILKTEEAKFSTKQIAVLALTIGPIWFVSE 180

Query: 181 YFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTTVGK 240
           YFTNAALARTSVATTTILFSTSGLFTLI+DAC+ERQSLTIVN VAV VSMAGV MTTVGK
Sbjct: 181 YFTNAALARTSVATTTILFSTSGLFTLILDACLERQSLTIVNAVAVVVSMAGVVMTTVGK 240

Query: 241 TWARDEAQSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVG 300
           TWA+DE QSSS GHGKHS+VGD FALLSA TDGLYYVLLKKYAGEEGEKVDMQKFLGYVG
Sbjct: 241 TWAKDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKVDMQKFLGYVG 300

Query: 301 LFTLTALWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGVVWTSPL 360
           LFTLT LWWL+WPLRAIGIEPKF IPQSTKVAE+VLANCFVANFVSDYFWAMGVVWTSPL
Sbjct: 301 LFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFVANFVSDYFWAMGVVWTSPL 360

Query: 361 VAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLKLRKKFF 420
           VAALGASLTIPLAM+GDMVLH RHYSLVYI GS+QVFLGF+IAN SDWISPKLKLRKKFF
Sbjct: 361 VAALGASLTIPLAMIGDMVLHDRHYSLVYIFGSIQVFLGFIIANFSDWISPKLKLRKKFF 418

BLAST of Spg021806 vs. ExPASy TrEMBL
Match: A0A6J1C0M9 (uncharacterized transporter C405.03c-like OS=Momordica charantia OX=3673 GN=LOC111006993 PE=4 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.7e-180
Identity = 340/420 (80.95%), Postives = 374/420 (89.05%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAW+YKGGLILLIAVVVIWVSSAEITQSIF DYEHPFV+TY+GTSMLVAYLAIAFIKECI
Sbjct: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60

Query: 61  MKLYRSHFRKGNSKKVAEI-QPPAIEERTSQSSSIEEDNNNNNNNIISEVENGDVHNCVV 120
           +KL+R+HF  GNSKKVAE+ QP  +EE+++  SS    NNNNNNN ISEV +G   +CVV
Sbjct: 61  VKLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPSS---PNNNNNNNNISEVGDGSEVHCVV 120

Query: 121 NIECEEEEEVRVNKN--GGCECEGENGGMRLKTEEITKFCTKQIAVLALAIGPIWFVSEY 180
            I+C EEE V V+K+  GGCECE +   M LKT+E TKF     AVLAL IGPIWFVSEY
Sbjct: 121 QIQC-EEEVVSVDKDNYGGCECENQEVAMALKTKE-TKF-----AVLALTIGPIWFVSEY 180

Query: 181 FTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTTVGKT 240
           FTNAALARTSVATTTILFSTSGLFTLIIDA +ERQSLTIVN+VAV VS+AGVAMTT+GKT
Sbjct: 181 FTNAALARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTIGKT 240

Query: 241 WARDEAQSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGL 300
           WA+D+  S+SS HGKHS+VGD FALLSA TDGLYYVLLKKYAGEEGEK+DMQKFLGYVGL
Sbjct: 241 WAKDQPHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGL 300

Query: 301 FTLTALWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGVVWTSPLV 360
           FTLT LWWL+WPLRAIGIEPKFAIP+STKVAE+VLANCF+ NF+SDYFWAMGVVWTSPLV
Sbjct: 301 FTLTTLWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLV 360

Query: 361 AALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLKLRKKFFN 418
           AALGASLTIPLAMLGDMVLHGRHYSLVYI GS+QVFLGFMIANLSDWISPKLKLR +FFN
Sbjct: 361 AALGASLTIPLAMLGDMVLHGRHYSLVYIFGSLQVFLGFMIANLSDWISPKLKLRSRFFN 410

BLAST of Spg021806 vs. TAIR 10
Match: AT4G32140.1 (EamA-like transporter family )

HSP 1 Score: 387.1 bits (993), Expect = 1.8e-107
Identity = 215/417 (51.56%), Postives = 288/417 (69.06%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           M W+YK GL L+  VV+IWV+SAE+TQ IFT Y+ PF VTY+G S+++ YL +AF+K+ +
Sbjct: 1   MGWRYKAGLFLIGTVVIIWVTSAEVTQDIFTAYKQPFAVTYLGASLMIVYLPVAFLKDWL 60

Query: 61  MKLYRSHFRKGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNIISEVENGDVHNCVVN 120
            +                     ++ R+S+S+ I    ++++  + S + +  +   +  
Sbjct: 61  CRY--------------------LDRRSSKSNKIPALTDDSSVELGSPLRHKIIEMGLQG 120

Query: 121 IECEEEEEVRVNKNGGCECEGENGGMRLKTEEI------TKFCTKQIAVLALAIGPIWFV 180
              +++ E  ++ +   E E E   +    EE+       +  TKQIA+  L + PIWFV
Sbjct: 121 TITKKDSEEHLSSH---EEEDERPLIGKVREEVQTLKQRKEITTKQIAMYGLYLAPIWFV 180

Query: 181 SEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTTV 240
           +EY +NAALARTSVA+TT+L STSGLFTL I   + + +L +  VVAVFVSMAGV MTT+
Sbjct: 181 TEYLSNAALARTSVASTTVLSSTSGLFTLFIGVFLGQDTLNLSKVVAVFVSMAGVVMTTL 240

Query: 241 GKTWARDEAQSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGY 300
           GKTWA DE+Q +SS +G+ S +GD+F LLSA + GL+ VLLKK+AGEEGE VD+QK  GY
Sbjct: 241 GKTWASDESQLNSSLNGERSLMGDLFGLLSAVSYGLFTVLLKKFAGEEGEGVDVQKLFGY 300

Query: 301 VGLFTLTALWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGVVWTS 360
           +GLFTL ALWWLVWPL A+GIEPKF IP S KV E+VLAN F+ + +SDYFWA+ VVWT+
Sbjct: 301 IGLFTLVALWWLVWPLTALGIEPKFTIPHSVKVDEVVLANGFIGSVLSDYFWALSVVWTT 360

Query: 361 PLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLKL 412
           PLVA LG SLTIPLAM+ DM++HGRHYS +YILGS QVF GF+IAN+SD  S KL L
Sbjct: 361 PLVATLGMSLTIPLAMVADMMIHGRHYSAIYILGSTQVFAGFVIANISDLFSKKLGL 394

BLAST of Spg021806 vs. TAIR 10
Match: AT3G07080.1 (EamA-like transporter family )

HSP 1 Score: 122.9 bits (307), Expect = 6.3e-28
Identity = 106/409 (25.92%), Postives = 198/409 (48.41%), Query Frame = 0

Query: 3   WKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECIMK 62
           W++  GLI L AV  IW++++ + QS+      PF++T++  S+ V YL +  I   +  
Sbjct: 6   WRWVLGLIYLFAVATIWIAASFVVQSVVEAGVSPFLITFICNSLFVVYLPLFEIGRYLED 65

Query: 63  LYRS--HFRKGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNIISEVE----NGDVHN 122
            Y S   +R   S  +  ++           S ++ D   ++  ++ E E       + +
Sbjct: 66  AYGSLLFWRSKRSHLMELVESEKAVLLGKDVSGVKSDATESSGLVVREQEISEGGNGIES 125

Query: 123 CVVNIECEEEEEVRVNKNGGCECEGENGGMRLKTEEITKFCTKQIAVLALAIGPIWFVSE 182
            + NIE E +  V V+       +G +G      +E  ++   ++A ++L I P WF+++
Sbjct: 126 GLENIELEIDASVNVS-------DGASGVSNKGLDEKGRWTRMRVAKVSLVICPFWFLAQ 185

Query: 183 YFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTTVGK 242
              N +L  T+V + TIL S S LFT ++      +  T + + +V + M+G  + ++G 
Sbjct: 186 LTFNVSLKYTTVTSNTILSSASSLFTFLVSLIFLGEKFTWLKLFSVLLCMSGTIIVSMG- 245

Query: 243 TWARDEAQSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGE---KVDMQKFLG 302
                +++S S+   K+  +GD+ +L+SA    +Y  L++K   ++ E   +V M +FLG
Sbjct: 246 -----DSESKSNAVAKNPLLGDILSLVSAALYAVYITLIRKKLPDDDERNGRVSMAQFLG 305

Query: 303 YVGLFTLTALWWLVWPLRAIGIEPKFAIPQST-KVAEIVLANCFVANFVSDYFWAMGVVW 362
           ++GLF     +  + P   +    +      T K   +V+    + N +SDY WA  V+ 
Sbjct: 306 FLGLFN---FFIFLPPALILNFTKRERFNALTLKQFGLVVGKGLLDNVLSDYLWAKAVLL 365

Query: 363 TSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANL 402
           T+  VA  G ++ +PLA + D  L G   S    +G+  V +GF   N+
Sbjct: 366 TTTTVATAGLTIQVPLAAIVDS-LSGNKPSFTDYIGAAAVMVGFAGINI 397