Spg021775 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg021775
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionNon-specific serine/threonine protein kinase
Locationscaffold2: 5713532 .. 5718680 (-)
RNA-Seq ExpressionSpg021775
SyntenySpg021775
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCACTTCCAGAGACGTCCAAGATATCATTTCAAAGCTATCTTCGGAAAAAGCCAAAGCTCGCGAGGTAATAACCATGTTCTTATGTGTTCGACTCACTCTTCGTCTGGGCTTTATGAATATGTTGAGAAAACTGTGTATGCCCGGCTCAAGACATGCATGATGCACAAAGCTTCTATTCTACAGTGCATCTCTGTAGTTTCTGAGCTCTGAGAAGTTTTATTTGATGTTTTTGCTGTACATTCAATCAATAGGGAGTTCGAAATAGCATTTCAGAACCCATGTAGTTTCCTCAGGGAAATGCTGAGTATTATATTTCTTAGCATCCAGTTCTCACAACTTGTATCCTTAAAAATAATCTAATGTTCATCTAGTAGATCAATCATCTTAACTTAAGCGATCATTATATTCATGTATTTTTTTCGCGTGCTTACAGGAAGGGATAAAGTTGTTGAATACATGGCTAGAGGGAGAAAAAGCAATTGATTTCTGTAAATTTATTGGACAGAACACTACTAAGCTCAAGCCAGAGGAAATTCCATCTCGTACGTTCAACAATCAAATTGACTTGAGCACATTTTCCTGTTATTCTTCAAACACCGTGTTAAAGTAATTACTCTTATTTTCTCTTCAGCTGAAACATGGCCTTTTATTACAAAATTGTTGATCCAATGTGTGTCAATGGAGATATCTTCAAGCAAGCGGCGATTGCCAAAGTTAATATTTGCGAAGACACTAAGAGTTGTCGTTCAAAAAGCAGAAGATAATAAGTTTTCTGGTGAGTGATTGAAATATGTCATTATTGTAGCTTTTGTTGCGAACTTGCAAGCACGTGTTTGTCCACAATCCTCATTTTCCCTAGTAACTTGGACATTTTGAAAGTTTAGGAACCTATTAGACACAAAATTAAAAATTTAGGGATCTACTTAGACACAAAATTGAAAGTTTATAAAGACCTATTAGACACTTTTTAAAGTTCAAAGATCAAATAGACACAAAATTGAAAGTTCAGAGACTAAACTTGTAATTTAACCTTCTTTTATTTTATTTTAAACACGATGAAAAGTCTGTTATCTTCTTTCAAAAAACAGATAAATTTAGAAAACATTTCTCTGAGTTCTAGTCTTTTTTTTTTTTTGAATGGAAAGTTTCATTTTTTGTTAAAAAAAAAATTAGACAATGATTCTCCAAAACAGATCTCGTATTTGTTGTTTTTTGAACCACAAATGAAATAGACCCTTTAATTTCAATATTTTGAGAAAGATAAAGTAGAAGAACTTCTTCATGTTCTTGGCGAGGGAAATATTTATGAACCACATGGTTCAACATTCATACTTAAAATTACTTTTGGTTATTAGTTGTGGTTCGTCATTGGTTTAATTTTTCAGGTTGTAGCCTTTCTATTATTTTTAAGCAATGGAATTCCTTCTTTTGTAAATATTACCTGAGCTTTCTTGACAGGATATCTAAACGTTATACTTGTGATGCTCGTTTTTCTTGTCCCAGGCAACGTAGTACCCTTGATATCTGTGGTTAAGATGCTCTTCAGTCATGCATGGGACATCTTGAGTACTATACCGTGCTTTCAATCTGAGTATGGGATCATAATTAGGCATTTAGTAGCTGTAAAAGATTATCGTTTTCATTTAAGGAAGCGCATATATTGCAGTAAGTTCTGTCATAACCTCAACCTGAGAGATGTTTATTCTTATGATTTATGCTTGATGTTTAATTTCAACTCTACAATGACTCATCATTTGGATTACTATATGATGTTTAATTTCATCTCAACTTCTCCATTCCATGTTGAAAGACCTCATGCTTTTATACTTGGAGAAGGTGGAAGGAAGCTTAGTTGGGAAAAGTGATAATCATTATACTCCAAAAGAGGAGCTCTTTCGGTGCATATTAACACTCCATTCACTTTTGGAAAATCCTCCTGGTGACTTCCCAGATAGTATTAGGCAAGAAATTGTAAATGGAATTGCTAAGATTTTCTCTCTCGTGAGGTATGATAATACTACCCAATTGTTACCATTTTGATTCTTGTGCATCTCCTATGACTTGTTAACTTGATCTTGGTGTTTCTATCATTGGTCTCTTTTAGTGAATGAATACATTTTTCTCTTTTCTTGGTCAGGGAGGAAGGAAAAGTGTCACGCAAACTTATTGAGTGTGTAAATACATACTTGTTAAAGGATGGTCCCAATCTAGGAAGTAACTTATTGAAGATCCATAATTCTGTGCAACAATTCGTGTTTCGGTGTTGGATGGTGACTCATGATCGAGCTCTAAAGGTATGAAATAAGTTGGACAAAATTAACTATTAGTTCTTTTCATTTATTGTTTTTAAAACATTTTCCTGACTTGTCACTTTTGCAGGATGCTCTTGTCTTATATGGAAGGTTACAAATAAGTTTAACAAGAGGACCTGATGATGAGAGGTTTTTGATAGAACAACTACTCGATGTACTTTATAAGGAACTAGATCAAAGCAGTATATCTAATGTTGGGGTGCCTTGGTTTGTATTTTCATATTTTCTATTGATTATCCTTTTTCCTTATATCTCATTTAGTTGAAGCTTGTTTATCATGTGATAATGTATGATAATTGGAAATATTTGAGTTTTCAGGTCTGATACAAATAAAGATGATAAATTTGGAACTTTGAGTAGCTCCCATTGCGGGTTGGTAGAACTTGGTGCAGCTGTACTCTATCGGGTATTCTAAACATACTGTGCTTCCTTATTGATTTACTATGCTACATTGACGGGAAGTTGTTATTTTGACTCACATCTAATGTACAATACGAAGGTATTGCTCTAGTGAGATTCCAATTCCCAAATAAGTATCCAAAATAATTTGTTACAACTTTTGGGATATTGTTTCCATTACTCATCCGTTAATCTATTGGATTTTCTTATATTCCATTTTAACGTTTCTGCTATCATAGCTTTTTATCTTTTAATTTTAGTAATGAGTTCTTGAAATCCTGTTGCTTGTAGTACTTTTTATATTGTAGAGATCTTAGGATTATAATTTAGTTGTTTGAATGGTTGTAGTTTATGGGCACTAGAAATGGATCTGACTGGTTGCGCATGGATTTTATTTATTTAGGCATGTGTTACCTCTACAAAAGCAATATCAATTGAAAAGCGGGTTAAAAGGGACCCTGCTAGTGCTCATCTTAAAGAGGCACTTGCTAAAGGAAAATGGTTATGGTAAATTTCTAAGCTATGTGATTATGAAAACATGATCTTGCATTCCCTGGTTCATTTGTTAATTTGGCACGGGGGATTATTTTTGTGTGACTGGATTAATTATCTTGTAGGAACGCGACCTTCTGTTGTCTTGTTCGAAATTACCATTCCCGCATCAGCAAGGATCTTTTCACCTACTGGTTTGAGGCAATTTGCTTAGGTTTCGAGAGGTATTTAAGATTTCATGTTGTAAATCTGTTTTTCTGTGCTATCAAATTCTTTATTTGTCTTATTGTCATCTTTATTTGGATAATTTTTTAATCATAGTTGTTGTTTCCTCTTTAAATTTCATCAAGCACTACCGAGGTCGGCTCAAGATATAGACAAATGAGGCAATTGCCTTAGGCCTCTAACTTTAGAGGAATTTATTTTCTTCCAAAGCCACATATTACCTTAGGCCGGGCCTACATATAATAAAATTGACAAAGATTGCATAATGGAACTCAAAATAAATGTAGATAGATGTGAAATTGAATGATTAAAATATACAAAATTAAAGTACCAAATATAAATTTTGCTTAAAATGTTCACCAAGCTCACATAGGCCGGCCATGCAATCTTTATATTAAGTCTACTAGTTTCTAGAGTAGTGCATGTTACAATAATATCATGTTAAAATTTAATTTGCACCTCATCTAGTTTGATATGACTTATGTGATATACTATCCAGATTAGACGACTTTTGCATAAAATCATTGTCTTTCATAAAAGAATTTATCAACTGTTAGCATCTTCTGAAATTCTTCTAAAAAAAATCTTCTGAAATTTGTTCTTCTTGTGTTTGACATGAAATTACTAGTTGCACGCATTTTCTTCATGCGTATATAATACAACGTGATACATGCTAATTAAAACTAGAAAACACAATCGTATTGTTCATGGTAATCAAAGCTACAAAAGATAATCATAATCTTCATGCTAACCAAAACTGCAAATAATACATGTGAAAAAAGCATGTGACAATTTCAATCGATCTAATTTATTTATTTTTTGAGCATCTTGCAGAATATTGAAAGATGCAAATGTGGGGCATTCTTATGATGGTTTATTGTGGACATTGCGGTAAGCTAGCATAACTTTTTATGTCTATCCAAAATTTTGGAATATACAAATCTGCAGTCAATTTAATTCCATTTTTGCTGCTTGGTTCATTCACATGATTGTTTATTTATTATTTCGAGCGAAAGTATATACTTCAGTTCCCTGTCCCATTGAGCTCATATATCTTTATATCCACATGAAGGAGTTTGCAAGAACTTTCTGTAGTGTTATTGGATTCTACTGAACGGTTAGAAAGTTCATCTGAGGATAAGGTATATTCCTCTTTTCATTATATTCCTCTTGTTTTTGTTTTGTTTTATGGGAAAGCAAAAGAAGAAGAGCCAAGCTAAAAGTGGGGTTTAAAAAACTTAAAAAAAAAGATTTCCTAACTTTTGGTAATAAAAAATAATAAATAATTTGAAAATAAATTTGAATTAAGAGCCTAGAGAAAGTTCAAACATCTTCCTACGAACTATTTACTTTTAAAAAACATTCTCATACTCCTCAAATTCCTTTTAGGGTTTTTTTTCTTCCATTTCTCTAATCATGGTTGTGATTCGATTGGCATCTGAAGATGCCAAGTTGTTGCATCGAGCTCGGGCTACTTCGTTTGCCATCGATTTGGTCTCCAACAACAACCAGTCAATGGCTGCTGACTTTTGGTCCGTCAAGATCAAGTATGGTAGCACCCTTGACGATGACCAACATAATGTCGATGCTGTTGAGACTCTATCTGCTGGGAATTTGCATGTCGCTTCCGATTACAGTTCTGAGAAGGATGAAGTAAAACCAAATGCTGAAGTTGTGACATCAATGCTAGGTCTCCAAAGTTATTGGGATTCTTAA

mRNA sequence

ATGGTCACTTCCAGAGACGTCCAAGATATCATTTCAAAGCTATCTTCGGAAAAAGCCAAAGCTCGCGAGGAAGGGATAAAGTTGTTGAATACATGGCTAGAGGGAGAAAAAGCAATTGATTTCTGTAAATTTATTGGACAGAACACTACTAAGCTCAAGCCAGAGGAAATTCCATCTCCTGAAACATGGCCTTTTATTACAAAATTGTTGATCCAATGTGTGTCAATGGAGATATCTTCAAGCAAGCGGCGATTGCCAAAGTTAATATTTGCGAAGACACTAAGAGTTGTCGTTCAAAAAGCAGAAGATAATAAGTTTTCTGGCAACGTAGTACCCTTGATATCTGTGGTTAAGATGCTCTTCAGTCATGCATGGGACATCTTGAGTACTATACCGTGCTTTCAATCTGAGTATGGGATCATAATTAGGCATTTAGTAGCTGTAAAAGATTATCGTTTTCATTTAAGGAAGCGCATATATTGCAACCTCATGCTTTTATACTTGGAGAAGGTGGAAGGAAGCTTAGTTGGGAAAAGTGATAATCATTATACTCCAAAAGAGGAGCTCTTTCGGTGCATATTAACACTCCATTCACTTTTGGAAAATCCTCCTGGTGACTTCCCAGATAGTATTAGGCAAGAAATTGTAAATGGAATTGCTAAGATTTTCTCTCTCGTGAGGGAGGAAGGAAAAGTGTCACGCAAACTTATTGAGTGTGTAAATACATACTTGTTAAAGGATGGTCCCAATCTAGGAAGTAACTTATTGAAGATCCATAATTCTGTGCAACAATTCGTGTTTCGGTGTTGGATGGTGACTCATGATCGAGCTCTAAAGGATGCTCTTGTCTTATATGGAAGGTTACAAATAAGTTTAACAAGAGGACCTGATGATGAGAGGTTTTTGATAGAACAACTACTCGATGTACTTTATAAGGAACTAGATCAAAGCAGTATATCTAATGTTGGGGTGCCTTGGTCTGATACAAATAAAGATGATAAATTTGGAACTTTGAGTAGCTCCCATTGCGGGTTGGTAGAACTTGGTGCAGCTGTACTCTATCGGGCATGTGTTACCTCTACAAAAGCAATATCAATTGAAAAGCGGGTTAAAAGGGACCCTGCTAGTGCTCATCTTAAAGAGGCACTTGCTAAAGGAAAATGGTTATGGAACGCGACCTTCTGTTGTCTTGTTCGAAATTACCATTCCCGCATCAGCAAGGATCTTTTCACCTACTGGTTTGAGGCAATTTGCTTAGGTTTCGAGAGAATATTGAAAGATGCAAATGTGGGGCATTCTTATGATGGTTTATTGTGGACATTGCGGAGTTTGCAAGAACTTTCTGTAGTGTTATTGGATTCTACTGAACGGTTAGAAAGTTCATCTGAGGATAAGGGTTTTTTTTCTTCCATTTCTCTAATCATGGTTGTGATTCGATTGGCATCTGAAGATGCCAAGTTGTTGCATCGAGCTCGGGCTACTTCGTTTGCCATCGATTTGGTCTCCAACAACAACCAGTCAATGGCTGCTGACTTTTGGTCCGTCAAGATCAAGTATGGTAGCACCCTTGACGATGACCAACATAATGTCGATGCTGTTGAGACTCTATCTGCTGGGAATTTGCATGTCGCTTCCGATTACAGTTCTGAGAAGGATGAAGTAAAACCAAATGCTGAAGTTGTGACATCAATGCTAGGTCTCCAAAGTTATTGGGATTCTTAA

Coding sequence (CDS)

ATGGTCACTTCCAGAGACGTCCAAGATATCATTTCAAAGCTATCTTCGGAAAAAGCCAAAGCTCGCGAGGAAGGGATAAAGTTGTTGAATACATGGCTAGAGGGAGAAAAAGCAATTGATTTCTGTAAATTTATTGGACAGAACACTACTAAGCTCAAGCCAGAGGAAATTCCATCTCCTGAAACATGGCCTTTTATTACAAAATTGTTGATCCAATGTGTGTCAATGGAGATATCTTCAAGCAAGCGGCGATTGCCAAAGTTAATATTTGCGAAGACACTAAGAGTTGTCGTTCAAAAAGCAGAAGATAATAAGTTTTCTGGCAACGTAGTACCCTTGATATCTGTGGTTAAGATGCTCTTCAGTCATGCATGGGACATCTTGAGTACTATACCGTGCTTTCAATCTGAGTATGGGATCATAATTAGGCATTTAGTAGCTGTAAAAGATTATCGTTTTCATTTAAGGAAGCGCATATATTGCAACCTCATGCTTTTATACTTGGAGAAGGTGGAAGGAAGCTTAGTTGGGAAAAGTGATAATCATTATACTCCAAAAGAGGAGCTCTTTCGGTGCATATTAACACTCCATTCACTTTTGGAAAATCCTCCTGGTGACTTCCCAGATAGTATTAGGCAAGAAATTGTAAATGGAATTGCTAAGATTTTCTCTCTCGTGAGGGAGGAAGGAAAAGTGTCACGCAAACTTATTGAGTGTGTAAATACATACTTGTTAAAGGATGGTCCCAATCTAGGAAGTAACTTATTGAAGATCCATAATTCTGTGCAACAATTCGTGTTTCGGTGTTGGATGGTGACTCATGATCGAGCTCTAAAGGATGCTCTTGTCTTATATGGAAGGTTACAAATAAGTTTAACAAGAGGACCTGATGATGAGAGGTTTTTGATAGAACAACTACTCGATGTACTTTATAAGGAACTAGATCAAAGCAGTATATCTAATGTTGGGGTGCCTTGGTCTGATACAAATAAAGATGATAAATTTGGAACTTTGAGTAGCTCCCATTGCGGGTTGGTAGAACTTGGTGCAGCTGTACTCTATCGGGCATGTGTTACCTCTACAAAAGCAATATCAATTGAAAAGCGGGTTAAAAGGGACCCTGCTAGTGCTCATCTTAAAGAGGCACTTGCTAAAGGAAAATGGTTATGGAACGCGACCTTCTGTTGTCTTGTTCGAAATTACCATTCCCGCATCAGCAAGGATCTTTTCACCTACTGGTTTGAGGCAATTTGCTTAGGTTTCGAGAGAATATTGAAAGATGCAAATGTGGGGCATTCTTATGATGGTTTATTGTGGACATTGCGGAGTTTGCAAGAACTTTCTGTAGTGTTATTGGATTCTACTGAACGGTTAGAAAGTTCATCTGAGGATAAGGGTTTTTTTTCTTCCATTTCTCTAATCATGGTTGTGATTCGATTGGCATCTGAAGATGCCAAGTTGTTGCATCGAGCTCGGGCTACTTCGTTTGCCATCGATTTGGTCTCCAACAACAACCAGTCAATGGCTGCTGACTTTTGGTCCGTCAAGATCAAGTATGGTAGCACCCTTGACGATGACCAACATAATGTCGATGCTGTTGAGACTCTATCTGCTGGGAATTTGCATGTCGCTTCCGATTACAGTTCTGAGAAGGATGAAGTAAAACCAAATGCTGAAGTTGTGACATCAATGCTAGGTCTCCAAAGTTATTGGGATTCTTAA

Protein sequence

MVTSRDVQDIISKLSSEKAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTTKLKPEEIPSPETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKMLFSHAWDILSTIPCFQSEYGIIIRHLVAVKDYRFHLRKRIYCNLMLLYLEKVEGSLVGKSDNHYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVREEGKVSRKLIECVNTYLLKDGPNLGSNLLKIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRGPDDERFLIEQLLDVLYKELDQSSISNVGVPWSDTNKDDKFGTLSSSHCGLVELGAAVLYRACVTSTKAISIEKRVKRDPASAHLKEALAKGKWLWNATFCCLVRNYHSRISKDLFTYWFEAICLGFERILKDANVGHSYDGLLWTLRSLQELSVVLLDSTERLESSSEDKGFFSSISLIMVVIRLASEDAKLLHRARATSFAIDLVSNNNQSMAADFWSVKIKYGSTLDDDQHNVDAVETLSAGNLHVASDYSSEKDEVKPNAEVVTSMLGLQSYWDS
Homology
BLAST of Spg021775 vs. NCBI nr
Match: XP_038878896.1 (serine/threonine-protein kinase ATM isoform X2 [Benincasa hispida])

HSP 1 Score: 882.1 bits (2278), Expect = 2.6e-252
Identity = 436/476 (91.60%), Postives = 455/476 (95.59%), Query Frame = 0

Query: 1   MVTSRDVQDIISKLSSEKAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTTKLKPEEIPSP 60
           MVTSRDVQDIISKLSS+KAKAREEGIKLLNTWLEGEKAIDFCKFIGQNT KLKPEEIPSP
Sbjct: 1   MVTSRDVQDIISKLSSDKAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTAKLKPEEIPSP 60

Query: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML 120
           ETWPFITKLLIQCVSMEISSSKRR+PKLIFAKTLR V+QKAEDNKFSGN VPLISVVK+L
Sbjct: 61  ETWPFITKLLIQCVSMEISSSKRRVPKLIFAKTLRTVIQKAEDNKFSGNAVPLISVVKVL 120

Query: 121 FSHAWDILSTIPCFQSEYGIIIRHLVAVKDYRFHLRKRIYCNLMLLYLEKVEGSLVGKSD 180
           FSHAWD+LST+ CFQSEYGIIIRHL+AV+DYRFHLRKRIYCNL+LLYLEKVEGSLVGK+D
Sbjct: 121 FSHAWDVLSTVSCFQSEYGIIIRHLIAVRDYRFHLRKRIYCNLLLLYLEKVEGSLVGKND 180

Query: 181 NHYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVREEGKVSRKLIECV 240
           N YTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVR+EGKVSRKLIECV
Sbjct: 181 NLYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVRDEGKVSRKLIECV 240

Query: 241 NTYLLKDGPNLGSNLLKIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRGPDDER 300
           NTYLLKDGPNLGS+LL+IHNSVQ+FVFRCWMVTHDRALKDALVLYGRLQISLTRG DDER
Sbjct: 241 NTYLLKDGPNLGSSLLEIHNSVQKFVFRCWMVTHDRALKDALVLYGRLQISLTRGVDDER 300

Query: 301 FLIEQLLDVLYKELDQSSISNV-GVPWSDTNKDDKFGTLSSSHCGLVELGAAVLYRACVT 360
            LIEQLLDVLYKELDQSSIS+V GVPWSD NKDDKFGTLSSSHCGLVEL AAVLYRACVT
Sbjct: 301 VLIEQLLDVLYKELDQSSISSVGGVPWSDANKDDKFGTLSSSHCGLVELAAAVLYRACVT 360

Query: 361 STKAISIEKRVKRDPASAHLKEALAKGKWLWNATFCCLVRNYHSRISKDLFTYWFEAICL 420
           S KAIS EKRVKRDPAS HLKEALA+GKWLWNATFCCL+RNYHSRISKDLFTYWFEAICL
Sbjct: 361 SPKAISTEKRVKRDPASVHLKEALAEGKWLWNATFCCLIRNYHSRISKDLFTYWFEAICL 420

Query: 421 GFERILKDANVGHSYDGLLWTLRSLQELSVVLLDSTERLESSSEDKGFFSSISLIM 476
            FERILKDANVGHSYDGLLWTLRSLQELS VLLDSTER ES SEDKG+ S  S +M
Sbjct: 421 DFERILKDANVGHSYDGLLWTLRSLQELSAVLLDSTERSESLSEDKGWQSIWSCLM 476

BLAST of Spg021775 vs. NCBI nr
Match: XP_023531465.1 (serine/threonine-protein kinase ATM [Cucurbita pepo subsp. pepo])

HSP 1 Score: 879.0 bits (2270), Expect = 2.2e-251
Identity = 435/465 (93.55%), Postives = 446/465 (95.91%), Query Frame = 0

Query: 1   MVTSRDVQDIISKLSSEKAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTTKLKPEEIPSP 60
           MVTSRDVQDI+SKLSS KAKAREEGIKLLNTWLEGEKA DFCKFIGQ T KLKPEEIP P
Sbjct: 1   MVTSRDVQDIVSKLSSVKAKAREEGIKLLNTWLEGEKANDFCKFIGQRTAKLKPEEIPPP 60

Query: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML 120
           ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML
Sbjct: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML 120

Query: 121 FSHAWDILSTIPCFQSEYGIIIRHLVAVKDYRFHLRKRIYCNLMLLYLEKVEGSLVGKSD 180
           FSH WD+LSTIPCFQSEYGIIIRHLVAV+DYRFHLRKRIYCNL+LLYLEKVEGSLVGK+D
Sbjct: 121 FSHIWDVLSTIPCFQSEYGIIIRHLVAVRDYRFHLRKRIYCNLVLLYLEKVEGSLVGKND 180

Query: 181 NHYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVREEGKVSRKLIECV 240
           N YTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVR+EGKVSRKLIECV
Sbjct: 181 NLYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVRDEGKVSRKLIECV 240

Query: 241 NTYLLKDGPNLGSNLLKIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRGPDDER 300
           NTYLLKDGPNLGSNLL+IHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTR  DDER
Sbjct: 241 NTYLLKDGPNLGSNLLEIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRA-DDER 300

Query: 301 FLIEQLLDVLYKELDQSSISNVGVPWSDTNKDDKFGTLSSSHCGLVELGAAVLYRACVTS 360
           F +EQLLDVLYKELDQSSIS+VGVPWSDTNKDDKFGTLSSSHCGLVEL A VLYRACVTS
Sbjct: 301 FFVEQLLDVLYKELDQSSISSVGVPWSDTNKDDKFGTLSSSHCGLVELAATVLYRACVTS 360

Query: 361 TKAISIEKRVKRDPASAHLKEALAKGKWLWNATFCCLVRNYHSRISKDLFTYWFEAICLG 420
           TKAIS EKRVKRDPAS HLKE LAKGKWLWNA FCCLVRNYHSRISKDLFTYWFEAIC G
Sbjct: 361 TKAISTEKRVKRDPASVHLKEELAKGKWLWNAAFCCLVRNYHSRISKDLFTYWFEAICSG 420

Query: 421 FERILKDANVGHSYDGLLWTLRSLQELSVVLLDSTERLESSSEDK 466
           FERILKDANVGHSYDGLLWTLRSLQELS VL+DSTER ESSSE+K
Sbjct: 421 FERILKDANVGHSYDGLLWTLRSLQELSSVLIDSTERSESSSEEK 464

BLAST of Spg021775 vs. NCBI nr
Match: XP_038878895.1 (serine/threonine-protein kinase ATM isoform X1 [Benincasa hispida])

HSP 1 Score: 877.5 bits (2266), Expect = 6.4e-251
Identity = 432/466 (92.70%), Postives = 449/466 (96.35%), Query Frame = 0

Query: 1   MVTSRDVQDIISKLSSEKAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTTKLKPEEIPSP 60
           MVTSRDVQDIISKLSS+KAKAREEGIKLLNTWLEGEKAIDFCKFIGQNT KLKPEEIPSP
Sbjct: 1   MVTSRDVQDIISKLSSDKAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTAKLKPEEIPSP 60

Query: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML 120
           ETWPFITKLLIQCVSMEISSSKRR+PKLIFAKTLR V+QKAEDNKFSGN VPLISVVK+L
Sbjct: 61  ETWPFITKLLIQCVSMEISSSKRRVPKLIFAKTLRTVIQKAEDNKFSGNAVPLISVVKVL 120

Query: 121 FSHAWDILSTIPCFQSEYGIIIRHLVAVKDYRFHLRKRIYCNLMLLYLEKVEGSLVGKSD 180
           FSHAWD+LST+ CFQSEYGIIIRHL+AV+DYRFHLRKRIYCNL+LLYLEKVEGSLVGK+D
Sbjct: 121 FSHAWDVLSTVSCFQSEYGIIIRHLIAVRDYRFHLRKRIYCNLLLLYLEKVEGSLVGKND 180

Query: 181 NHYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVREEGKVSRKLIECV 240
           N YTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVR+EGKVSRKLIECV
Sbjct: 181 NLYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVRDEGKVSRKLIECV 240

Query: 241 NTYLLKDGPNLGSNLLKIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRGPDDER 300
           NTYLLKDGPNLGS+LL+IHNSVQ+FVFRCWMVTHDRALKDALVLYGRLQISLTRG DDER
Sbjct: 241 NTYLLKDGPNLGSSLLEIHNSVQKFVFRCWMVTHDRALKDALVLYGRLQISLTRGVDDER 300

Query: 301 FLIEQLLDVLYKELDQSSISNV-GVPWSDTNKDDKFGTLSSSHCGLVELGAAVLYRACVT 360
            LIEQLLDVLYKELDQSSIS+V GVPWSD NKDDKFGTLSSSHCGLVEL AAVLYRACVT
Sbjct: 301 VLIEQLLDVLYKELDQSSISSVGGVPWSDANKDDKFGTLSSSHCGLVELAAAVLYRACVT 360

Query: 361 STKAISIEKRVKRDPASAHLKEALAKGKWLWNATFCCLVRNYHSRISKDLFTYWFEAICL 420
           S KAIS EKRVKRDPAS HLKEALA+GKWLWNATFCCL+RNYHSRISKDLFTYWFEAICL
Sbjct: 361 SPKAISTEKRVKRDPASVHLKEALAEGKWLWNATFCCLIRNYHSRISKDLFTYWFEAICL 420

Query: 421 GFERILKDANVGHSYDGLLWTLRSLQELSVVLLDSTERLESSSEDK 466
            FERILKDANVGHSYDGLLWTLRSLQELS VLLDSTER ES SEDK
Sbjct: 421 DFERILKDANVGHSYDGLLWTLRSLQELSAVLLDSTERSESLSEDK 466

BLAST of Spg021775 vs. NCBI nr
Match: XP_038878897.1 (serine/threonine-protein kinase ATM isoform X3 [Benincasa hispida])

HSP 1 Score: 877.5 bits (2266), Expect = 6.4e-251
Identity = 432/466 (92.70%), Postives = 449/466 (96.35%), Query Frame = 0

Query: 1   MVTSRDVQDIISKLSSEKAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTTKLKPEEIPSP 60
           MVTSRDVQDIISKLSS+KAKAREEGIKLLNTWLEGEKAIDFCKFIGQNT KLKPEEIPSP
Sbjct: 1   MVTSRDVQDIISKLSSDKAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTAKLKPEEIPSP 60

Query: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML 120
           ETWPFITKLLIQCVSMEISSSKRR+PKLIFAKTLR V+QKAEDNKFSGN VPLISVVK+L
Sbjct: 61  ETWPFITKLLIQCVSMEISSSKRRVPKLIFAKTLRTVIQKAEDNKFSGNAVPLISVVKVL 120

Query: 121 FSHAWDILSTIPCFQSEYGIIIRHLVAVKDYRFHLRKRIYCNLMLLYLEKVEGSLVGKSD 180
           FSHAWD+LST+ CFQSEYGIIIRHL+AV+DYRFHLRKRIYCNL+LLYLEKVEGSLVGK+D
Sbjct: 121 FSHAWDVLSTVSCFQSEYGIIIRHLIAVRDYRFHLRKRIYCNLLLLYLEKVEGSLVGKND 180

Query: 181 NHYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVREEGKVSRKLIECV 240
           N YTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVR+EGKVSRKLIECV
Sbjct: 181 NLYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVRDEGKVSRKLIECV 240

Query: 241 NTYLLKDGPNLGSNLLKIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRGPDDER 300
           NTYLLKDGPNLGS+LL+IHNSVQ+FVFRCWMVTHDRALKDALVLYGRLQISLTRG DDER
Sbjct: 241 NTYLLKDGPNLGSSLLEIHNSVQKFVFRCWMVTHDRALKDALVLYGRLQISLTRGVDDER 300

Query: 301 FLIEQLLDVLYKELDQSSISNV-GVPWSDTNKDDKFGTLSSSHCGLVELGAAVLYRACVT 360
            LIEQLLDVLYKELDQSSIS+V GVPWSD NKDDKFGTLSSSHCGLVEL AAVLYRACVT
Sbjct: 301 VLIEQLLDVLYKELDQSSISSVGGVPWSDANKDDKFGTLSSSHCGLVELAAAVLYRACVT 360

Query: 361 STKAISIEKRVKRDPASAHLKEALAKGKWLWNATFCCLVRNYHSRISKDLFTYWFEAICL 420
           S KAIS EKRVKRDPAS HLKEALA+GKWLWNATFCCL+RNYHSRISKDLFTYWFEAICL
Sbjct: 361 SPKAISTEKRVKRDPASVHLKEALAEGKWLWNATFCCLIRNYHSRISKDLFTYWFEAICL 420

Query: 421 GFERILKDANVGHSYDGLLWTLRSLQELSVVLLDSTERLESSSEDK 466
            FERILKDANVGHSYDGLLWTLRSLQELS VLLDSTER ES SEDK
Sbjct: 421 DFERILKDANVGHSYDGLLWTLRSLQELSAVLLDSTERSESLSEDK 466

BLAST of Spg021775 vs. NCBI nr
Match: XP_023004525.1 (serine/threonine-protein kinase ATM isoform X2 [Cucurbita maxima])

HSP 1 Score: 877.5 bits (2266), Expect = 6.4e-251
Identity = 434/465 (93.33%), Postives = 447/465 (96.13%), Query Frame = 0

Query: 1   MVTSRDVQDIISKLSSEKAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTTKLKPEEIPSP 60
           MVTSRDVQDI+SKLSS KAKAREEGIKLLNTWLEGEKA DFCKFIGQ T KLKPEEIP P
Sbjct: 1   MVTSRDVQDIVSKLSSVKAKAREEGIKLLNTWLEGEKANDFCKFIGQRTAKLKPEEIPPP 60

Query: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML 120
           ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML
Sbjct: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML 120

Query: 121 FSHAWDILSTIPCFQSEYGIIIRHLVAVKDYRFHLRKRIYCNLMLLYLEKVEGSLVGKSD 180
           FSH WD+LSTIPCFQSEYGIIIRHLVAV+DYRFHLRKRIYCNL+LLYLEKVEGSLVGK+D
Sbjct: 121 FSHIWDVLSTIPCFQSEYGIIIRHLVAVRDYRFHLRKRIYCNLVLLYLEKVEGSLVGKND 180

Query: 181 NHYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVREEGKVSRKLIECV 240
           + YTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVR+EGKVSRKLIECV
Sbjct: 181 SLYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVRDEGKVSRKLIECV 240

Query: 241 NTYLLKDGPNLGSNLLKIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRGPDDER 300
           NTYLLKDGPNLGSNLL+IHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTR  DDER
Sbjct: 241 NTYLLKDGPNLGSNLLEIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRA-DDER 300

Query: 301 FLIEQLLDVLYKELDQSSISNVGVPWSDTNKDDKFGTLSSSHCGLVELGAAVLYRACVTS 360
           F +EQLLDVLYKELDQSSIS+VGVPWSDTNKDDKFGTLSSSHCGLVEL AAVLYRACVTS
Sbjct: 301 FFVEQLLDVLYKELDQSSISSVGVPWSDTNKDDKFGTLSSSHCGLVELAAAVLYRACVTS 360

Query: 361 TKAISIEKRVKRDPASAHLKEALAKGKWLWNATFCCLVRNYHSRISKDLFTYWFEAICLG 420
           TKA+S EKRVKRDPAS HLKE LAKGKWLWNA FCCLVRNYHSRISKDLFTYWFEAIC G
Sbjct: 361 TKAMSTEKRVKRDPASVHLKEELAKGKWLWNAAFCCLVRNYHSRISKDLFTYWFEAICSG 420

Query: 421 FERILKDANVGHSYDGLLWTLRSLQELSVVLLDSTERLESSSEDK 466
           FERILKDANVGHSYDGLLWTLRSLQELS VL+DSTER ESSSE+K
Sbjct: 421 FERILKDANVGHSYDGLLWTLRSLQELSSVLIDSTERSESSSEEK 464

BLAST of Spg021775 vs. ExPASy Swiss-Prot
Match: Q9M3G7 (Serine/threonine-protein kinase ATM OS=Arabidopsis thaliana OX=3702 GN=ATM PE=1 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 5.8e-154
Identity = 279/494 (56.48%), Postives = 356/494 (72.06%), Query Frame = 0

Query: 12   SKLSSE-KAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTTKLKPEEIPSPETWPFITKLL 71
            +KL SE      ++G+KLLNTWLEGE++I FC+F+ QNT KLK +EIP+ ETWPF+ KLL
Sbjct: 824  AKLKSEITGLLEKDGVKLLNTWLEGERSITFCRFLSQNTAKLKLDEIPNAETWPFLVKLL 883

Query: 72   IQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKMLFSHAWDILST 131
            +QCVSME+S SKRR+PK  FAKTLRVVVQ+ E+ KF G   PL+S+ K LF+H  DILS 
Sbjct: 884  LQCVSMEVSGSKRRMPKPTFAKTLRVVVQRTEETKFPGVQFPLLSMAKTLFTHVHDILSN 943

Query: 132  IPCFQSEYGIIIRHLVAVKDYRFHLRKRIYCNLMLLYLEKVEGSLVGKSDNHYTPKEELF 191
             P FQSEYG I+RHL+ +K+YRF +RKR Y +L+LLY+E+ E     K+   ++ KEE F
Sbjct: 944  TPSFQSEYGTILRHLLEIKEYRFQMRKRTYSSLVLLYMERAETGFCEKNSGQHSQKEEAF 1003

Query: 192  RCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVREEGKVSRKLIECVNTYLLKDGPN 251
            R ILTL SLLEN PGDFPD +R+EIVNG+  IFS VR+EGK+SRKLIECVNT+LLKDGPN
Sbjct: 1004 RYILTLQSLLENSPGDFPDDLREEIVNGLIHIFSSVRDEGKLSRKLIECVNTFLLKDGPN 1063

Query: 252  LGSNLLKIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRGPDDERFLIEQLLDVL 311
            LGS  L+IHN+V+QFVFRCW+ THD+ LK+ LV YGRLQ++LTR   +   L+EQLLDV+
Sbjct: 1064 LGSLSLEIHNAVEQFVFRCWLTTHDKNLKEILVSYGRLQLNLTRDSSESSSLVEQLLDVV 1123

Query: 312  YKELDQSSISNVGVPWSDTNKDDKFGTLSSSHCGLVELGAAVLYRACVTSTKAISIEKRV 371
             +ELD  S S+    W DT KD+K G LSS    LVEL A V YRACV +++    EKR 
Sbjct: 1124 TRELDLGS-SSSSASWGDTTKDEKLGALSSYQNSLVELAAHVFYRACVNTSRPSLSEKRA 1183

Query: 372  KRDPASAHLKEALAKGKWLWNATFCCLVRNYHSRISKDLFTYWFEAICLGFERILKDANV 431
            +R   +  + +AL +GKWLW A F CLVRNY +RI+ DL  YWFEAIC  F+R+L+DA++
Sbjct: 1184 RRQHIAMRMVDALTEGKWLWCAAFGCLVRNYCARINMDLLIYWFEAICTNFQRLLEDASM 1243

Query: 432  GHSYDGLLWTLRSLQELSV------VLLDSTERLESSSE-DKGF-------------FSS 483
              SYDGLLWTLRSLQ LS       + +D ++   SSSE D+G+             FSS
Sbjct: 1244 RRSYDGLLWTLRSLQGLSSGLSLPDITMDISKSSASSSELDRGWQSIWSSLIHGLATFSS 1303

BLAST of Spg021775 vs. ExPASy TrEMBL
Match: A0A6J1KZS2 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111497793 PE=4 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 3.1e-251
Identity = 434/465 (93.33%), Postives = 447/465 (96.13%), Query Frame = 0

Query: 1   MVTSRDVQDIISKLSSEKAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTTKLKPEEIPSP 60
           MVTSRDVQDI+SKLSS KAKAREEGIKLLNTWLEGEKA DFCKFIGQ T KLKPEEIP P
Sbjct: 1   MVTSRDVQDIVSKLSSVKAKAREEGIKLLNTWLEGEKANDFCKFIGQRTAKLKPEEIPPP 60

Query: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML 120
           ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML
Sbjct: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML 120

Query: 121 FSHAWDILSTIPCFQSEYGIIIRHLVAVKDYRFHLRKRIYCNLMLLYLEKVEGSLVGKSD 180
           FSH WD+LSTIPCFQSEYGIIIRHLVAV+DYRFHLRKRIYCNL+LLYLEKVEGSLVGK+D
Sbjct: 121 FSHIWDVLSTIPCFQSEYGIIIRHLVAVRDYRFHLRKRIYCNLVLLYLEKVEGSLVGKND 180

Query: 181 NHYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVREEGKVSRKLIECV 240
           + YTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVR+EGKVSRKLIECV
Sbjct: 181 SLYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVRDEGKVSRKLIECV 240

Query: 241 NTYLLKDGPNLGSNLLKIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRGPDDER 300
           NTYLLKDGPNLGSNLL+IHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTR  DDER
Sbjct: 241 NTYLLKDGPNLGSNLLEIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRA-DDER 300

Query: 301 FLIEQLLDVLYKELDQSSISNVGVPWSDTNKDDKFGTLSSSHCGLVELGAAVLYRACVTS 360
           F +EQLLDVLYKELDQSSIS+VGVPWSDTNKDDKFGTLSSSHCGLVEL AAVLYRACVTS
Sbjct: 301 FFVEQLLDVLYKELDQSSISSVGVPWSDTNKDDKFGTLSSSHCGLVELAAAVLYRACVTS 360

Query: 361 TKAISIEKRVKRDPASAHLKEALAKGKWLWNATFCCLVRNYHSRISKDLFTYWFEAICLG 420
           TKA+S EKRVKRDPAS HLKE LAKGKWLWNA FCCLVRNYHSRISKDLFTYWFEAIC G
Sbjct: 361 TKAMSTEKRVKRDPASVHLKEELAKGKWLWNAAFCCLVRNYHSRISKDLFTYWFEAICSG 420

Query: 421 FERILKDANVGHSYDGLLWTLRSLQELSVVLLDSTERLESSSEDK 466
           FERILKDANVGHSYDGLLWTLRSLQELS VL+DSTER ESSSE+K
Sbjct: 421 FERILKDANVGHSYDGLLWTLRSLQELSSVLIDSTERSESSSEEK 464

BLAST of Spg021775 vs. ExPASy TrEMBL
Match: A0A6J1KUU2 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111497793 PE=4 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 3.1e-251
Identity = 434/465 (93.33%), Postives = 447/465 (96.13%), Query Frame = 0

Query: 1   MVTSRDVQDIISKLSSEKAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTTKLKPEEIPSP 60
           MVTSRDVQDI+SKLSS KAKAREEGIKLLNTWLEGEKA DFCKFIGQ T KLKPEEIP P
Sbjct: 1   MVTSRDVQDIVSKLSSVKAKAREEGIKLLNTWLEGEKANDFCKFIGQRTAKLKPEEIPPP 60

Query: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML 120
           ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML
Sbjct: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML 120

Query: 121 FSHAWDILSTIPCFQSEYGIIIRHLVAVKDYRFHLRKRIYCNLMLLYLEKVEGSLVGKSD 180
           FSH WD+LSTIPCFQSEYGIIIRHLVAV+DYRFHLRKRIYCNL+LLYLEKVEGSLVGK+D
Sbjct: 121 FSHIWDVLSTIPCFQSEYGIIIRHLVAVRDYRFHLRKRIYCNLVLLYLEKVEGSLVGKND 180

Query: 181 NHYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVREEGKVSRKLIECV 240
           + YTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVR+EGKVSRKLIECV
Sbjct: 181 SLYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVRDEGKVSRKLIECV 240

Query: 241 NTYLLKDGPNLGSNLLKIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRGPDDER 300
           NTYLLKDGPNLGSNLL+IHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTR  DDER
Sbjct: 241 NTYLLKDGPNLGSNLLEIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRA-DDER 300

Query: 301 FLIEQLLDVLYKELDQSSISNVGVPWSDTNKDDKFGTLSSSHCGLVELGAAVLYRACVTS 360
           F +EQLLDVLYKELDQSSIS+VGVPWSDTNKDDKFGTLSSSHCGLVEL AAVLYRACVTS
Sbjct: 301 FFVEQLLDVLYKELDQSSISSVGVPWSDTNKDDKFGTLSSSHCGLVELAAAVLYRACVTS 360

Query: 361 TKAISIEKRVKRDPASAHLKEALAKGKWLWNATFCCLVRNYHSRISKDLFTYWFEAICLG 420
           TKA+S EKRVKRDPAS HLKE LAKGKWLWNA FCCLVRNYHSRISKDLFTYWFEAIC G
Sbjct: 361 TKAMSTEKRVKRDPASVHLKEELAKGKWLWNAAFCCLVRNYHSRISKDLFTYWFEAICSG 420

Query: 421 FERILKDANVGHSYDGLLWTLRSLQELSVVLLDSTERLESSSEDK 466
           FERILKDANVGHSYDGLLWTLRSLQELS VL+DSTER ESSSE+K
Sbjct: 421 FERILKDANVGHSYDGLLWTLRSLQELSSVLIDSTERSESSSEEK 464

BLAST of Spg021775 vs. ExPASy TrEMBL
Match: A0A6J1EKR8 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111435229 PE=4 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.5e-250
Identity = 433/465 (93.12%), Postives = 445/465 (95.70%), Query Frame = 0

Query: 1   MVTSRDVQDIISKLSSEKAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTTKLKPEEIPSP 60
           MVTSRDVQDI+SKLSS KAKAREEGIKLLNTWLEGEKA DFCKFIGQ T KLKPEEIP P
Sbjct: 1   MVTSRDVQDIVSKLSSVKAKAREEGIKLLNTWLEGEKANDFCKFIGQRTAKLKPEEIPPP 60

Query: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML 120
           ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVV LISVVKML
Sbjct: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVSLISVVKML 120

Query: 121 FSHAWDILSTIPCFQSEYGIIIRHLVAVKDYRFHLRKRIYCNLMLLYLEKVEGSLVGKSD 180
           FSH WD+LSTIPCFQSEYGIIIRHLVA +DYRFHLRKRIYCNL+LLYLEKVEGSLVGK+D
Sbjct: 121 FSHVWDVLSTIPCFQSEYGIIIRHLVAARDYRFHLRKRIYCNLVLLYLEKVEGSLVGKND 180

Query: 181 NHYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVREEGKVSRKLIECV 240
           N YTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVR+EGKVSRKLIECV
Sbjct: 181 NLYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVRDEGKVSRKLIECV 240

Query: 241 NTYLLKDGPNLGSNLLKIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRGPDDER 300
           NTYLLKDGPNLGSNLL+IHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTR  DDER
Sbjct: 241 NTYLLKDGPNLGSNLLEIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRA-DDER 300

Query: 301 FLIEQLLDVLYKELDQSSISNVGVPWSDTNKDDKFGTLSSSHCGLVELGAAVLYRACVTS 360
           F +EQLLDVLYK+LDQSSIS+VGVPWSDTNKDDKFGTLSSSHCGLVEL AAVLYRACVTS
Sbjct: 301 FFVEQLLDVLYKDLDQSSISSVGVPWSDTNKDDKFGTLSSSHCGLVELAAAVLYRACVTS 360

Query: 361 TKAISIEKRVKRDPASAHLKEALAKGKWLWNATFCCLVRNYHSRISKDLFTYWFEAICLG 420
           TKAIS EKRVKRDPAS HLKE LAKGKWLWNA FCCLVRNYHSRISKDLFTYWFEAIC G
Sbjct: 361 TKAISTEKRVKRDPASVHLKEELAKGKWLWNAAFCCLVRNYHSRISKDLFTYWFEAICSG 420

Query: 421 FERILKDANVGHSYDGLLWTLRSLQELSVVLLDSTERLESSSEDK 466
           FERILKDANVGHSYDGLLWTLRSLQELS VL+DSTER ESSSE+K
Sbjct: 421 FERILKDANVGHSYDGLLWTLRSLQELSSVLIDSTERSESSSEEK 464

BLAST of Spg021775 vs. ExPASy TrEMBL
Match: A0A6J1EJV2 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111435229 PE=4 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.5e-250
Identity = 433/465 (93.12%), Postives = 445/465 (95.70%), Query Frame = 0

Query: 1   MVTSRDVQDIISKLSSEKAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTTKLKPEEIPSP 60
           MVTSRDVQDI+SKLSS KAKAREEGIKLLNTWLEGEKA DFCKFIGQ T KLKPEEIP P
Sbjct: 1   MVTSRDVQDIVSKLSSVKAKAREEGIKLLNTWLEGEKANDFCKFIGQRTAKLKPEEIPPP 60

Query: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML 120
           ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVV LISVVKML
Sbjct: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVSLISVVKML 120

Query: 121 FSHAWDILSTIPCFQSEYGIIIRHLVAVKDYRFHLRKRIYCNLMLLYLEKVEGSLVGKSD 180
           FSH WD+LSTIPCFQSEYGIIIRHLVA +DYRFHLRKRIYCNL+LLYLEKVEGSLVGK+D
Sbjct: 121 FSHVWDVLSTIPCFQSEYGIIIRHLVAARDYRFHLRKRIYCNLVLLYLEKVEGSLVGKND 180

Query: 181 NHYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVREEGKVSRKLIECV 240
           N YTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVR+EGKVSRKLIECV
Sbjct: 181 NLYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVRDEGKVSRKLIECV 240

Query: 241 NTYLLKDGPNLGSNLLKIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRGPDDER 300
           NTYLLKDGPNLGSNLL+IHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTR  DDER
Sbjct: 241 NTYLLKDGPNLGSNLLEIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRA-DDER 300

Query: 301 FLIEQLLDVLYKELDQSSISNVGVPWSDTNKDDKFGTLSSSHCGLVELGAAVLYRACVTS 360
           F +EQLLDVLYK+LDQSSIS+VGVPWSDTNKDDKFGTLSSSHCGLVEL AAVLYRACVTS
Sbjct: 301 FFVEQLLDVLYKDLDQSSISSVGVPWSDTNKDDKFGTLSSSHCGLVELAAAVLYRACVTS 360

Query: 361 TKAISIEKRVKRDPASAHLKEALAKGKWLWNATFCCLVRNYHSRISKDLFTYWFEAICLG 420
           TKAIS EKRVKRDPAS HLKE LAKGKWLWNA FCCLVRNYHSRISKDLFTYWFEAIC G
Sbjct: 361 TKAISTEKRVKRDPASVHLKEELAKGKWLWNAAFCCLVRNYHSRISKDLFTYWFEAICSG 420

Query: 421 FERILKDANVGHSYDGLLWTLRSLQELSVVLLDSTERLESSSEDK 466
           FERILKDANVGHSYDGLLWTLRSLQELS VL+DSTER ESSSE+K
Sbjct: 421 FERILKDANVGHSYDGLLWTLRSLQELSSVLIDSTERSESSSEEK 464

BLAST of Spg021775 vs. ExPASy TrEMBL
Match: A0A1S3BWI4 (serine/threonine-protein kinase ATM isoform X3 OS=Cucumis melo OX=3656 GN=LOC103493908 PE=4 SV=1)

HSP 1 Score: 869.4 bits (2245), Expect = 8.4e-249
Identity = 427/465 (91.83%), Postives = 442/465 (95.05%), Query Frame = 0

Query: 1   MVTSRDVQDIISKLSSEKAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTTKLKPEEIPSP 60
           M+TSRDVQDIISKLSS+KAK REEGIKLLNTWLEGEKAIDFCKFIGQNT KLKPEEIPSP
Sbjct: 1   MITSRDVQDIISKLSSDKAKTREEGIKLLNTWLEGEKAIDFCKFIGQNTAKLKPEEIPSP 60

Query: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKML 120
           ETWPFITKLLIQCVSMEISSSKRRLPKL+FAKTLR VVQKAE NKFSGN +PLISVVK+L
Sbjct: 61  ETWPFITKLLIQCVSMEISSSKRRLPKLMFAKTLRAVVQKAEANKFSGNALPLISVVKVL 120

Query: 121 FSHAWDILSTIPCFQSEYGIIIRHLVAVKDYRFHLRKRIYCNLMLLYLEKVEGSLVGKSD 180
           FSH WD+LST PCFQSEYGIIIRHLVAV+DYRFHLRKRIYCNLMLLYLEKVEGSLVGK+D
Sbjct: 121 FSHVWDVLSTTPCFQSEYGIIIRHLVAVRDYRFHLRKRIYCNLMLLYLEKVEGSLVGKND 180

Query: 181 NHYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVREEGKVSRKLIECV 240
           N YTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGI KIFSLVR+EGKVSRKLIECV
Sbjct: 181 NLYTPKEELFRCILTLHSLLENPPGDFPDSIRQEIVNGIVKIFSLVRDEGKVSRKLIECV 240

Query: 241 NTYLLKDGPNLGSNLLKIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRGPDDER 300
           NTYLLKDG NLGS+LL+IHNSVQ FVFRCWMVTHDRALKDALVLYGRLQISLTRG DDE 
Sbjct: 241 NTYLLKDGSNLGSSLLEIHNSVQTFVFRCWMVTHDRALKDALVLYGRLQISLTRGADDES 300

Query: 301 FLIEQLLDVLYKELDQSSISNVGVPWSDTNKDDKFGTLSSSHCGLVELGAAVLYRACVTS 360
            LIEQLLDVLYKELDQSSI +VGVPWSD NKDDKFGTLSSSHCGLVEL AAVLYRACVTS
Sbjct: 301 VLIEQLLDVLYKELDQSSIFSVGVPWSDANKDDKFGTLSSSHCGLVELAAAVLYRACVTS 360

Query: 361 TKAISIEKRVKRDPASAHLKEALAKGKWLWNATFCCLVRNYHSRISKDLFTYWFEAICLG 420
           TKAIS EKRVKRDPAS HLKEAL +GKWLWNA FCCL++NYHSRISKDLFTYWFEAICLG
Sbjct: 361 TKAISTEKRVKRDPASVHLKEALGEGKWLWNAAFCCLIQNYHSRISKDLFTYWFEAICLG 420

Query: 421 FERILKDANVGHSYDGLLWTLRSLQELSVVLLDSTERLESSSEDK 466
           FERILKDANVGHSYDGLLWTLRSLQELS VLLDSTER ESSSEDK
Sbjct: 421 FERILKDANVGHSYDGLLWTLRSLQELSGVLLDSTERSESSSEDK 465

BLAST of Spg021775 vs. TAIR 10
Match: AT3G48190.1 (ataxia-telangiectasia mutated )

HSP 1 Score: 545.8 bits (1405), Expect = 4.1e-155
Identity = 279/494 (56.48%), Postives = 356/494 (72.06%), Query Frame = 0

Query: 12   SKLSSE-KAKAREEGIKLLNTWLEGEKAIDFCKFIGQNTTKLKPEEIPSPETWPFITKLL 71
            +KL SE      ++G+KLLNTWLEGE++I FC+F+ QNT KLK +EIP+ ETWPF+ KLL
Sbjct: 824  AKLKSEITGLLEKDGVKLLNTWLEGERSITFCRFLSQNTAKLKLDEIPNAETWPFLVKLL 883

Query: 72   IQCVSMEISSSKRRLPKLIFAKTLRVVVQKAEDNKFSGNVVPLISVVKMLFSHAWDILST 131
            +QCVSME+S SKRR+PK  FAKTLRVVVQ+ E+ KF G   PL+S+ K LF+H  DILS 
Sbjct: 884  LQCVSMEVSGSKRRMPKPTFAKTLRVVVQRTEETKFPGVQFPLLSMAKTLFTHVHDILSN 943

Query: 132  IPCFQSEYGIIIRHLVAVKDYRFHLRKRIYCNLMLLYLEKVEGSLVGKSDNHYTPKEELF 191
             P FQSEYG I+RHL+ +K+YRF +RKR Y +L+LLY+E+ E     K+   ++ KEE F
Sbjct: 944  TPSFQSEYGTILRHLLEIKEYRFQMRKRTYSSLVLLYMERAETGFCEKNSGQHSQKEEAF 1003

Query: 192  RCILTLHSLLENPPGDFPDSIRQEIVNGIAKIFSLVREEGKVSRKLIECVNTYLLKDGPN 251
            R ILTL SLLEN PGDFPD +R+EIVNG+  IFS VR+EGK+SRKLIECVNT+LLKDGPN
Sbjct: 1004 RYILTLQSLLENSPGDFPDDLREEIVNGLIHIFSSVRDEGKLSRKLIECVNTFLLKDGPN 1063

Query: 252  LGSNLLKIHNSVQQFVFRCWMVTHDRALKDALVLYGRLQISLTRGPDDERFLIEQLLDVL 311
            LGS  L+IHN+V+QFVFRCW+ THD+ LK+ LV YGRLQ++LTR   +   L+EQLLDV+
Sbjct: 1064 LGSLSLEIHNAVEQFVFRCWLTTHDKNLKEILVSYGRLQLNLTRDSSESSSLVEQLLDVV 1123

Query: 312  YKELDQSSISNVGVPWSDTNKDDKFGTLSSSHCGLVELGAAVLYRACVTSTKAISIEKRV 371
             +ELD  S S+    W DT KD+K G LSS    LVEL A V YRACV +++    EKR 
Sbjct: 1124 TRELDLGS-SSSSASWGDTTKDEKLGALSSYQNSLVELAAHVFYRACVNTSRPSLSEKRA 1183

Query: 372  KRDPASAHLKEALAKGKWLWNATFCCLVRNYHSRISKDLFTYWFEAICLGFERILKDANV 431
            +R   +  + +AL +GKWLW A F CLVRNY +RI+ DL  YWFEAIC  F+R+L+DA++
Sbjct: 1184 RRQHIAMRMVDALTEGKWLWCAAFGCLVRNYCARINMDLLIYWFEAICTNFQRLLEDASM 1243

Query: 432  GHSYDGLLWTLRSLQELSV------VLLDSTERLESSSE-DKGF-------------FSS 483
              SYDGLLWTLRSLQ LS       + +D ++   SSSE D+G+             FSS
Sbjct: 1244 RRSYDGLLWTLRSLQGLSSGLSLPDITMDISKSSASSSELDRGWQSIWSSLIHGLATFSS 1303

BLAST of Spg021775 vs. TAIR 10
Match: AT1G66680.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 87.8 bits (216), Expect = 3.1e-17
Identity = 53/107 (49.53%), Postives = 72/107 (67.29%), Query Frame = 0

Query: 475 MVVIRLASEDAKLL--HRARA-----TSFAIDLVSNNNQSMAADFWSVKIKYGSTLDDDQ 534
           M  IRL  E+ +     +ARA     T+    L S++++S+AAD WS+K +YGSTLDDDQ
Sbjct: 1   MAGIRLLPEEPETTPQQQARAAAAVTTTTTDSLASDDDRSIAADSWSIKSEYGSTLDDDQ 60

Query: 535 HNVDAVETLSAGNLHVASDYSSEKDEVKPNAE-VVTSMLGLQSYWDS 574
            + DA E LS+ N  V+SDYSS+K+E  P+A+    SMLGLQSYWD+
Sbjct: 61  RHADAAEALSSANFRVSSDYSSDKEE--PDADGGGQSMLGLQSYWDA 105

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878896.12.6e-25291.60serine/threonine-protein kinase ATM isoform X2 [Benincasa hispida][more]
XP_023531465.12.2e-25193.55serine/threonine-protein kinase ATM [Cucurbita pepo subsp. pepo][more]
XP_038878895.16.4e-25192.70serine/threonine-protein kinase ATM isoform X1 [Benincasa hispida][more]
XP_038878897.16.4e-25192.70serine/threonine-protein kinase ATM isoform X3 [Benincasa hispida][more]
XP_023004525.16.4e-25193.33serine/threonine-protein kinase ATM isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9M3G75.8e-15456.48Serine/threonine-protein kinase ATM OS=Arabidopsis thaliana OX=3702 GN=ATM PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1KZS23.1e-25193.33Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1KUU23.1e-25193.33Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1EKR81.5e-25093.12Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1EJV21.5e-25093.12Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A1S3BWI48.4e-24991.83serine/threonine-protein kinase ATM isoform X3 OS=Cucumis melo OX=3656 GN=LOC103... [more]
Match NameE-valueIdentityDescription
AT3G48190.14.1e-15556.48ataxia-telangiectasia mutated [more]
AT1G66680.13.1e-1749.53S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 4..24
NoneNo IPR availablePANTHERPTHR37079:SF5SERINE/THREONINE-PROTEIN KINASE ATM-LIKE ISOFORM X1coord: 1..462
IPR038980Serine/threonine-protein kinase ATM, plantPANTHERPTHR37079SERINE/THREONINE-PROTEIN KINASE ATMcoord: 1..462

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg021775.1Spg021775.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006281 DNA repair
biological_process GO:0006468 protein phosphorylation
biological_process GO:0006974 cellular response to DNA damage stimulus
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity