Spg021759 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg021759
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Nudix hydrolase 20
Location	scaffold2: 7144099 .. 7147598 (+)
RNA-Seq Expression	Spg021759
Synteny	Spg021759

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGACTTCTCCTCCAATTCAACCCAGCAAATTTCTCGCTTCACACAGATTTGGTAACTCTCCTGCTATCTCTTCCATTTGCAATGCTTCCATGTTTCTCCGAACGAGGTATCCAACGACATCCATATCTCGATCAACAGTACACCGCTCGATGACTTTCACCTCTCGTTCTATATCGCTCGCCAGCAGCAGTTTCACTTGGGAAGACGCTGTCCACATTTCTTTGCCAGAAAGCTTTCAGGATGATCCCTCAGATCTTACGGGCTATTTCGAGAAGGTCAAGCTCTGCAATCGCGGATCCGTAGGTCTCTTTCTCCTTTTTCTGTTTGTTTGGTTGTGCAAAGTATACATATCGGCAGTAATTGTATGCTTTAATTCGTTTTACATTTCAAATTCAGCGCAGTAAAAGCTATAGACTGACAAAATGTGGTTCCATGTCTGTTTGGAATTGACTAAATCTTCTAACAGGACATGCAATACGAATTTCTTCCGTTTGTCATCGATGACCAAATTGTTGGTTATGTGCACCACGGGTGAGGTTCCTACTTTATATCTTTCAGGATACACTACACACATCGAAGAAATGATCCGTCCTTGGAGGCTTTACTAATTCTTTATACTAATTTCATCTAAGTTTGCTTGTGTGCTGTTGCTACTTGCTATGTATAACGGATAGGTCCGATCAGTTTGTTTTAATGTAATTTATGCAGTTTTGCTAATCATCTGAAGAAGTATGCAAATGTGTTTACCTTCCCCCGAGATGATTCTGTTAAATTTGGCACTTGCTTAAGCTTGCATGAATCGTTAAAGACACCGGAAGATAGGACGCATGCAGTTGGAGATGTAGTCAAATGCTTGGGTGAAGAAGTTATTCCAGGTACACGGAACGAGGTACTGCATGTGATTCCAATTTAAAGCAATGTTTAATGCTCAAGTAATTTTAGAACAAGATAATGCTTCTTGTGGCTAACAGTCTACAACAATGAACTCTGTCAGCTGTACCCTGTGTCTTCGTCCTTTGGTGCGCCGGTTTTTTTCTCGCTTGAACGTGCTGCAGCTCCTTATTTTGGAATAAAGGTGTGAATGTTCCCCTTCTACAGTTTTGTGGAAACTTCTATTTTCTTGCTCTAATTTTCTGTATCAAACTCTCGAAGCCATGTTTTGATCAAACCTTTTCTTTTAGGCCCTTGAAAATTGTAGGCCCTACGCTTTATGGACTCTGTTTGTGAAGGTTTAGCTGTTAGGTCTGGATAAATTTGAAACCTTCAATATTTGATCGTCATTCTGAATATTTATAGTACTATTCAATACCCTTCTACATCGCTGGAAATAATTATCACTACTTGGCTACTCTAATTTCTACCAGCCTTCTCTTGGTGCTTTTCTTAGCACAGGTTTATGGAGTTCAGATGAATGGTTATGTTGAAAAGGATGGAAAGAAATTTTTGTGGATAGCAAAAAGAAGTCAAATGAAGCAAACTTTTCCTGGAATGCTGGATGAATTAGTAGCTGGAGGGCTGGTTTGTAATAAATAATAACTTATTGTTTAGTTCTATTGCATGTCATGTCACACGTGCATCTGTACCGTTGAAATTTTAAAAAAACAGGTTTTTCCACCCCTTTTCTTCATGATTTGATGACACTGATTACATACTCCTTTCATCTTTCAAACATAGCCTTGCAATGTTTTCTGATTGATTGAGGATAAGACTTCCATTTTCTTTCTGAATGGTTGGTTCTCTTATTTCTTTTTGATTCTTTGCTAGATCCTTGTAGTTGTATGAAGATACTTTCTAGTATTACATTCCTTTATATGAAATTGAGCATGTTTGTGTTTCTGTCAATATGAATATGCCTGTTTTATTAAGCTTAGTTAAAGCAGCCAACAATATTTGATTTTTATTTGTGTTCGACTTTCAGTTTGGATAGTTAATAACAAAAACAGATAGGTTTTGGTACTGATGCATTCATTGACTTAGTAATAAATCCTTATCTAAATAACAGCGTATTATATTTTTTTATTGAGATCGAGATGTGAAGTTTGATAATTTCCTAGGAGCAGTAGAGTCTGCAAAAACACATTTTTATCAGTAGATTTGAGAAATGCAAATGCGAAGCTTTACAATGATGAGAGGAAAAAGAACCAAGAGATATTAGAAGTTAGAAATGTGCACTTGAATGCTGAACATCATTCCTATTGTACCTTTATAGCTAGCCCCTGACTTTATGATAGTGACAGCCTGAGGGCGTTCCTTGCGGGGAGAATCTGATGAAGGAATGTGAGGAGGAAGCAGGCATACCGAGATCAATATCAAAAAAGTAATTTCCTAACAAGAACTTGATTATAATTTAGCACCAATATAGCCTTATATTACAAGGCCATGGTAAACTTTCTTTTTTTGAGTTCAACATGTGGGAGTTTGAACCTTTGACCTCTTGGTTGATCGGATGTGCCTTAACCAGTTGAGCTAGCTATACTAACTAGGTGACTTCATAATGCTTTTGTTTCAGGGCCATACCAGTTGGTGCTGTTTCCTATTCAGACATCAAAGATTATAGTTACAAGAGAGATGTTCTATTTTGTTATGATTTAAAACTTCCTGAAAGTTTTATACCGATGAATCAAGGTTAGTTAATGTGATAAATTTTCATTTGCTTAATCATTCAAAATTTGGACGAGGTATTTTACATTGAATTTAGTCTAAACTGGCTCCAAATAATTCTCCTTTTTGCCCCATCCCCGGTGTATGAATGCACATTTTTAATATAAATCTGTCTAGTTAGAAATTCCATGCCACTAAATTTTGCTGACTAAACTTTTGGCAGATGGGGAGGTTGAAGGATTCAGGTTGTTGCCTGTGACAAATGTTGCAAATGTGATTCGGAGGACACAATTATTCAAGCATAATTGTGCTCTTGTCATCATTGATTTCCTATTTCGACATGGGTATGATATACTATTTTCTCTTTGGCTATACATTTGTTGCTCCATTATCTGCTTAAATTACAACACCAAGTGTTTATCTTGGTGGTAAAAATTCGTGGATTCAAACAAATTGTCATGGGTACAAAACTTCTCAAATCACTTGTAAATTGTCATAGGCCTTTTCTTTTACCCGTCTATGTGGCCAATCCCAATGACCATTAGTTCTCAAGGCATCCTTACGAGCATAGAGTTGTTGTAATTAATTTATTTTTGTTTGAAAAACTTGTAGGTACATTAAACCCGAAAGTTTGGGGTACTTGGAGCTACTACAAAGTTTGAAGAGTGGAACCTGCTTCTGAATAAATTCATTCGCCCAATGAGAGAAATTGGGCCAATCTTATTAACAATCCAACTCGAATGGTTCATATTGTTCATTGTTTGACCAGAATCTGCTTGCTCAATAGATGTATTTGTATATTCCAAAAAAAAAAAAGATGTATTTGTGAAGCCAAACAAACTGCCTTACTTGCTATTTCCTTTTTCTGAATGACAATACCTTTAAAAGTAGCCGC

mRNA sequence

ATGGCGACTTCTCCTCCAATTCAACCCAGCAAATTTCTCGCTTCACACAGATTTGGTAACTCTCCTGCTATCTCTTCCATTTGCAATGCTTCCATGTTTCTCCGAACGAGGTATCCAACGACATCCATATCTCGATCAACAGTACACCGCTCGATGACTTTCACCTCTCGTTCTATATCGCTCGCCAGCAGCAGTTTCACTTGGGAAGACGCTGTCCACATTTCTTTGCCAGAAAGCTTTCAGGATGATCCCTCAGATCTTACGGGCTATTTCGAGAAGGTCAAGCTCTGCAATCGCGGATCCGTAGGTCTCTTTCTCCTTTTTCTGTTTGTTTGGTTGTGCAAAGTATACATATCGGCAGTAATTGACATGCAATACGAATTTCTTCCGTTTGTCATCGATGACCAAATTGTTGGTTATGTGCACCACGGTTTTGCTAATCATCTGAAGAAGTATGCAAATGTGTTTACCTTCCCCCGAGATGATTCTGTTAAATTTGGCACTTGCTTAAGCTTGCATGAATCGTTAAAGACACCGGAAGATAGGACGCATGCAGTTGGAGATGTAGTCAAATGCTTGGGTGAAGAAGTTATTCCAGGTACACGGAACGAGCTGTACCCTGTGTCTTCGTCCTTTGGTGCGCCGGTTTTTTTCTCGCTTGAACGTGCTGCAGCTCCTTATTTTGGAATAAAGGTTTATGGAGTTCAGATGAATGGTTATGTTGAAAAGGATGGAAAGAAATTTTTGTGGATAGCAAAAAGAAGTCAAATGAAGCAAACTTTTCCTGGAATGCTGGATGAATTAGTAGCTGGAGGGCTGCCTGAGGGCGTTCCTTGCGGGGAGAATCTGATGAAGGAATGTGAGGAGGAAGCAGGCATACCGAGATCAATATCAAAAAAGGCCATACCAGTTGGTGCTGTTTCCTATTCAGACATCAAAGATTATAGTTACAAGAGAGATGTTCTATTTTGTTATGATTTAAAACTTCCTGAAAGTTTTATACCGATGAATCAAGATGGGGAGGTTGAAGGATTCAGGTTGTTGCCTGTGACAAATGTTGCAAATGTGATTCGGAGGACACAATTATTCAAGCATAATTGTGCTCTTGTCATCATTGATTTCCTATTTCGACATGGGTACATTAAACCCGAAAGTTTGGGGTACTTGGAGCTACTACAAAGTTTGAAGAGTGGAACCTGCTTCTGA

Coding sequence (CDS)

ATGGCGACTTCTCCTCCAATTCAACCCAGCAAATTTCTCGCTTCACACAGATTTGGTAACTCTCCTGCTATCTCTTCCATTTGCAATGCTTCCATGTTTCTCCGAACGAGGTATCCAACGACATCCATATCTCGATCAACAGTACACCGCTCGATGACTTTCACCTCTCGTTCTATATCGCTCGCCAGCAGCAGTTTCACTTGGGAAGACGCTGTCCACATTTCTTTGCCAGAAAGCTTTCAGGATGATCCCTCAGATCTTACGGGCTATTTCGAGAAGGTCAAGCTCTGCAATCGCGGATCCGTAGGTCTCTTTCTCCTTTTTCTGTTTGTTTGGTTGTGCAAAGTATACATATCGGCAGTAATTGACATGCAATACGAATTTCTTCCGTTTGTCATCGATGACCAAATTGTTGGTTATGTGCACCACGGTTTTGCTAATCATCTGAAGAAGTATGCAAATGTGTTTACCTTCCCCCGAGATGATTCTGTTAAATTTGGCACTTGCTTAAGCTTGCATGAATCGTTAAAGACACCGGAAGATAGGACGCATGCAGTTGGAGATGTAGTCAAATGCTTGGGTGAAGAAGTTATTCCAGGTACACGGAACGAGCTGTACCCTGTGTCTTCGTCCTTTGGTGCGCCGGTTTTTTTCTCGCTTGAACGTGCTGCAGCTCCTTATTTTGGAATAAAGGTTTATGGAGTTCAGATGAATGGTTATGTTGAAAAGGATGGAAAGAAATTTTTGTGGATAGCAAAAAGAAGTCAAATGAAGCAAACTTTTCCTGGAATGCTGGATGAATTAGTAGCTGGAGGGCTGCCTGAGGGCGTTCCTTGCGGGGAGAATCTGATGAAGGAATGTGAGGAGGAAGCAGGCATACCGAGATCAATATCAAAAAAGGCCATACCAGTTGGTGCTGTTTCCTATTCAGACATCAAAGATTATAGTTACAAGAGAGATGTTCTATTTTGTTATGATTTAAAACTTCCTGAAAGTTTTATACCGATGAATCAAGATGGGGAGGTTGAAGGATTCAGGTTGTTGCCTGTGACAAATGTTGCAAATGTGATTCGGAGGACACAATTATTCAAGCATAATTGTGCTCTTGTCATCATTGATTTCCTATTTCGACATGGGTACATTAAACCCGAAAGTTTGGGGTACTTGGAGCTACTACAAAGTTTGAAGAGTGGAACCTGCTTCTGA

Protein sequence

MATSPPIQPSKFLASHRFGNSPAISSICNASMFLRTRYPTTSISRSTVHRSMTFTSRSISLASSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSVGLFLLFLFVWLCKVYISAVIDMQYEFLPFVIDDQIVGYVHHGFANHLKKYANVFTFPRDDSVKFGTCLSLHESLKTPEDRTHAVGDVVKCLGEEVIPGTRNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGVPCGENLMKECEEEAGIPRSISKKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQSLKSGTCF

Homology

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BLAST of Spg021759 vs. NCBI nr
Match: XP_023530783.1 (nudix hydrolase 20, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023530784.1 nudix hydrolase 20, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 677.6 bits (1747), Expect = 6.8e-191
Identity = 343/403 (85.11%), Postives = 352/403 (87.34%), Query Frame = 0


Query: 1   MATSPPIQP--SKFLASHRFGNSPAISSICNASMFLRTRYPTTSISRSTVHRSMTFTSRS 60
           M T P IQP   K LASHRF NS  ISSI NASMF R  YPT     STVHRS TF  RS
Sbjct: 1   MVTCPRIQPMACKSLASHRFRNSLPISSISNASMFSRATYPTA----STVHRSKTFACRS 60

Query: 61  ISLASSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSVGLFLLFLFVWLCKVYI 120
           +S+ASS+FTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGS                 
Sbjct: 61  VSVASSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGS----------------- 120

Query: 121 SAVIDMQYEFLPFVIDDQIVGYVHHGFANHLKKYANVFTFPRDDSVKFGTCLSLHESLKT 180
               DMQ EFLPFVIDDQIVG+VHHGFA HLKKYANVFTFPRDDSV+ G CLSLHESLKT
Sbjct: 121 ----DMQSEFLPFVIDDQIVGHVHHGFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKT 180

Query: 181 PEDRTHAVGDVVKCLGEEVIPGTRNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMN 240
           PEDRTHAVG+VVKCLGEE+IPG RNE+YPV+SSFGAPVFFSLERAAAPYFGIKVYGVQMN
Sbjct: 181 PEDRTHAVGNVVKCLGEEIIPGIRNEMYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMN 240

Query: 241 GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGVPCGENLMKECEEEAGIPRSIS 300
           GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGV CGENLMKECEEEAGIPRSIS
Sbjct: 241 GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGVSCGENLMKECEEEAGIPRSIS 300

Query: 301 KKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIR 360
            KAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIP NQDGEVEGFRLLPVTNVANVIR
Sbjct: 301 NKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIR 360

Query: 361 RTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQSLKSGTCF 402
           RTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQSLKSGTCF
Sbjct: 361 RTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQSLKSGTCF 378

[Close]

BLAST of Spg021759 vs. NCBI nr
Match: XP_022933802.1 (nudix hydrolase 20, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 675.6 bits (1742), Expect = 2.6e-190
Identity = 342/403 (84.86%), Postives = 352/403 (87.34%), Query Frame = 0


Query: 1   MATSPPIQP--SKFLASHRFGNSPAISSICNASMFLRTRYPTTSISRSTVHRSMTFTSRS 60
           MAT P IQP   K LASHRF NS  ISSI NASMF R RYPT     ST+HRS TF  RS
Sbjct: 1   MATCPRIQPMACKSLASHRFRNSSPISSISNASMFSRARYPTA----STLHRSKTFACRS 60

Query: 61  ISLASSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSVGLFLLFLFVWLCKVYI 120
           +S+ASS+FTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGS                 
Sbjct: 61  VSVASSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGS----------------- 120

Query: 121 SAVIDMQYEFLPFVIDDQIVGYVHHGFANHLKKYANVFTFPRDDSVKFGTCLSLHESLKT 180
               DMQ EFLPFVIDDQIVG+VHHGFA HLKKYANVFTFPRDDSV+ G CLSLHESLKT
Sbjct: 121 ----DMQSEFLPFVIDDQIVGHVHHGFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKT 180

Query: 181 PEDRTHAVGDVVKCLGEEVIPGTRNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMN 240
           PEDRTHAVG+VVKCLGEE+IPG RNE+YPV+SSFGAPVFFSLERAAA YFGIKVYGVQMN
Sbjct: 181 PEDRTHAVGNVVKCLGEEIIPGIRNEMYPVTSSFGAPVFFSLERAAATYFGIKVYGVQMN 240

Query: 241 GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGVPCGENLMKECEEEAGIPRSIS 300
           GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGV CGENLMKECEEEAGIPRSIS
Sbjct: 241 GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGVSCGENLMKECEEEAGIPRSIS 300

Query: 301 KKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIR 360
            KAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIP NQDGEVEGFRLLPVTNVANVIR
Sbjct: 301 NKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIR 360

Query: 361 RTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQSLKSGTCF 402
           RTQLFKHNCALVIIDFLFRHGYIKPES GYLELLQSLKSGTCF
Sbjct: 361 RTQLFKHNCALVIIDFLFRHGYIKPESSGYLELLQSLKSGTCF 378

[Close]

BLAST of Spg021759 vs. NCBI nr
Match: XP_023002396.1 (nudix hydrolase 20, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 674.1 bits (1738), Expect = 7.5e-190
Identity = 340/403 (84.37%), Postives = 351/403 (87.10%), Query Frame = 0


Query: 1   MATSPPIQP--SKFLASHRFGNSPAISSICNASMFLRTRYPTTSISRSTVHRSMTFTSRS 60
           MAT P IQP   K LASH F NS  ISSI NASMF R  YPT     STVHRS TF  RS
Sbjct: 1   MATCPRIQPMACKSLASHPFRNSSPISSISNASMFSRATYPTA----STVHRSKTFACRS 60

Query: 61  ISLASSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSVGLFLLFLFVWLCKVYI 120
           +S+ASS+FTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGS                 
Sbjct: 61  VSIASSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGS----------------- 120

Query: 121 SAVIDMQYEFLPFVIDDQIVGYVHHGFANHLKKYANVFTFPRDDSVKFGTCLSLHESLKT 180
               DMQ EFLPFVIDDQIVG+VHHGFA HLKKYANVFTFPRDDSV+ G CLSLHESLKT
Sbjct: 121 ----DMQSEFLPFVIDDQIVGHVHHGFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKT 180

Query: 181 PEDRTHAVGDVVKCLGEEVIPGTRNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMN 240
           PEDRTHAVG+VVKCLGEE+IPG RNE+YPV+SSFGAPVFFSLERAAAPYFGIKVYGVQMN
Sbjct: 181 PEDRTHAVGNVVKCLGEEIIPGVRNEMYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMN 240

Query: 241 GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGVPCGENLMKECEEEAGIPRSIS 300
           GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEG+ CGENLMKECEEEAGIPRSIS
Sbjct: 241 GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGISCGENLMKECEEEAGIPRSIS 300

Query: 301 KKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIR 360
            KAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIP NQDGEVEGFRLLPVTNVANVIR
Sbjct: 301 NKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIR 360

Query: 361 RTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQSLKSGTCF 402
           RTQLFKHNCALVII+FLFRHGYIKPE LGYLELLQSLKSGTCF
Sbjct: 361 RTQLFKHNCALVIIEFLFRHGYIKPECLGYLELLQSLKSGTCF 378

[Close]

BLAST of Spg021759 vs. NCBI nr
Match: KAG6587948.1 (Nudix hydrolase 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 673.3 bits (1736), Expect = 1.3e-189
Identity = 342/403 (84.86%), Postives = 351/403 (87.10%), Query Frame = 0


Query: 1   MATSPPIQP--SKFLASHRFGNSPAISSICNASMFLRTRYPTTSISRSTVHRSMTFTSRS 60
           MAT P IQP   K LASHRF NS  ISSI NASMF R  YPT     STVHRS TF  RS
Sbjct: 1   MATCPRIQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTA----STVHRSKTFACRS 60

Query: 61  ISLASSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSVGLFLLFLFVWLCKVYI 120
           +S+ASS+FTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGS                 
Sbjct: 61  VSVASSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGS----------------- 120

Query: 121 SAVIDMQYEFLPFVIDDQIVGYVHHGFANHLKKYANVFTFPRDDSVKFGTCLSLHESLKT 180
               DMQ EFLPFVIDDQIVG+VHHGFA HLKKYANVFTFPRDDSV+ G CLSLHESLKT
Sbjct: 121 ----DMQSEFLPFVIDDQIVGHVHHGFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKT 180

Query: 181 PEDRTHAVGDVVKCLGEEVIPGTRNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMN 240
           PEDRTHAVG+VVKCLGEE+I G RNE+YPV+SSFGAPVFFSLERAAAPYFGIKVYGVQMN
Sbjct: 181 PEDRTHAVGNVVKCLGEEIIQGIRNEMYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMN 240

Query: 241 GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGVPCGENLMKECEEEAGIPRSIS 300
           GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGV C ENLMKECEEEAGIPRSIS
Sbjct: 241 GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGVSCEENLMKECEEEAGIPRSIS 300

Query: 301 KKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIR 360
            KAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIP NQDGEVEGFRLLPVTNVANVIR
Sbjct: 301 NKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIR 360

Query: 361 RTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQSLKSGTCF 402
           RTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQSLKSGTCF
Sbjct: 361 RTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQSLKSGTCF 378

[Close]

BLAST of Spg021759 vs. NCBI nr
Match: XP_038878620.1 (nudix hydrolase 20, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 664.8 bits (1714), Expect = 4.5e-187
Identity = 336/403 (83.37%), Postives = 347/403 (86.10%), Query Frame = 0


Query: 1   MATSPPI--QPSKFLASHRFGNSPAISSICNASMFLRTRYPTTSISRSTVHRSMTFTSRS 60
           MATSPPI     K LASH F  S A SSICNASMF  TR+ T         RSMTF  RS
Sbjct: 3   MATSPPIPHMACKSLASHTFCISHAFSSICNASMFSPTRFLT-----PPTFRSMTFAFRS 62

Query: 61  ISLASSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSVGLFLLFLFVWLCKVYI 120
           +S+ SSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGS                 
Sbjct: 63  VSVTSSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGS----------------- 122

Query: 121 SAVIDMQYEFLPFVIDDQIVGYVHHGFANHLKKYANVFTFPRDDSVKFGTCLSLHESLKT 180
               DMQ EFLPFVIDD I+GYVHHGF  HLKKYANVFTFPRDDSV+FG CL+LHESLKT
Sbjct: 123 ----DMQSEFLPFVIDDLILGYVHHGFGRHLKKYANVFTFPRDDSVEFGACLTLHESLKT 182

Query: 181 PEDRTHAVGDVVKCLGEEVIPGTRNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMN 240
           PEDRTHAVGDVVKCLGEEVIPGTRNELYPV+SSFG PVFFSLERAAAPYFGIKVYGVQMN
Sbjct: 183 PEDRTHAVGDVVKCLGEEVIPGTRNELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMN 242

Query: 241 GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGVPCGENLMKECEEEAGIPRSIS 300
           GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLP+G+PCGENLMKECEEEAGIPRSIS
Sbjct: 243 GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPQGIPCGENLMKECEEEAGIPRSIS 302

Query: 301 KKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIR 360
            KA+PVGAVSYSDIKDYSYKRDVLFCYDLKLPE+FIP NQDGEVEGFRLLPVTNVANVIR
Sbjct: 303 NKAMPVGAVSYSDIKDYSYKRDVLFCYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIR 362

Query: 361 RTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQSLKSGTCF 402
           RTQLFKHNCALVIIDFLFRHGYI PESLGYLELLQSLKSGTCF
Sbjct: 363 RTQLFKHNCALVIIDFLFRHGYIIPESLGYLELLQSLKSGTCF 379

[Close]

BLAST of Spg021759 vs. ExPASy Swiss-Prot
Match: Q8VXZ0 (Nudix hydrolase 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT20 PE=2 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 5.0e-112
Identity = 205/367 (55.86%), Postives = 261/367 (71.12%), Query Frame = 0


Query: 37  RYPTTSISRSTVHRSMTFTSRSISLASSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKL 96
           R+ ++S+S S +  S   ++ +    SSSFTW+D +     E    + SDLTG+FEK+  
Sbjct: 31  RWRSSSMSLSPLRHSRALSAATTVPISSSFTWDDVIETGRAEY---NSSDLTGFFEKINR 90

Query: 97  CNRGSVGLFLLFLFVWLCKVYISAVIDMQYEFLPFVIDDQIVGYVHHGFANHLKKYANVF 156
           CNRGS  L                      EF+PFVI++QIVGY+H  F  +L+++ ++F
Sbjct: 91  CNRGSEKL---------------------GEFIPFVIEEQIVGYIHKRFTEYLREFHDIF 150

Query: 157 TFPRDDSV--KFGTCLSLHESLKTPEDRTHAVGDVVKCLGEE-VIPGTRNELYPVSSSFG 216
           TF ++ S   +    ++L+  L+ PEDRT AV DV+K LG++ +IPG RNELYPV  SF 
Sbjct: 151 TFSQNGSCPDRVDGYVTLNLMLQKPEDRTRAVADVIKILGDKGIIPGIRNELYPVKPSFN 210

Query: 217 APVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGL 276
           APVFFSLERAAAPYFGIK YGV MNGYVE+DG+K LWI KRS  K T+PGMLD LVAGGL
Sbjct: 211 APVFFSLERAAAPYFGIKGYGVHMNGYVERDGQKLLWIGKRSLSKSTYPGMLDHLVAGGL 270

Query: 277 PEGVPCGENLMKECEEEAGIPRSISKKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESF 336
           P G+ CG NL+KECEEEAGI R+I+ +AI VGAVSY DI  Y +KRDVLFCYDL+LPE F
Sbjct: 271 PHGISCGGNLVKECEEEAGISRAIADRAIAVGAVSYLDIDQYCFKRDVLFCYDLELPEDF 330

Query: 337 IPMNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQ 396
           +P NQDGEVE F+L+PV  VA+VI++T  FK NC+LVIIDFLFRHG+I+PES GYL+L Q
Sbjct: 331 VPKNQDGEVESFKLIPVAQVASVIKKTSFFKANCSLVIIDFLFRHGFIRPESSGYLDLYQ 373

Query: 397 SLKSGTC 401
            L++  C
Sbjct: 391 RLRNRDC 373

[Close]

BLAST of Spg021759 vs. ExPASy Swiss-Prot
Match: P0C026 (Nudix hydrolase 24, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT24 PE=2 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.1e-103
Identity = 195/382 (51.05%), Postives = 258/382 (67.54%), Query Frame = 0


Query: 22  PAISSICNASMFLRT-RYPTTSISRS-TVHRSMTFTSRSISLASSSFTWEDAVHISLPES 81
           P  +S+ ++   + T ++ ++S SRS  +H S   +  ++ L S SFTW D    S  E 
Sbjct: 10  PTPTSLFSSHALIPTLQWRSSSSSRSPPLHISRVLSVETVPL-SPSFTWNDVFENSRKEY 69

Query: 82  FQDDPSDLTGYFEKVKLCNRGSVGLFLLFLFVWLCKVYISAVIDMQYEFLPFVIDDQIVG 141
              + SDLTG+ EKV  CNRG                     ++   EF+PFVI++QIVG
Sbjct: 70  VPQNSSDLTGFLEKVDRCNRG---------------------LEKLGEFIPFVIEEQIVG 129

Query: 142 YVHHGFANHLKKYANVFTFPRDDSVKFGTCLSLHESLKTPEDRTHAVGDVVKCLGEE-VI 201
           Y+H GF  +L+ + ++FTF      ++G  ++L+  L  PE+RT AV  V+K LG + +I
Sbjct: 130 YIHKGFTKYLRDFNDIFTFS-----QYGGHVTLNMMLDKPEERTRAVAHVIKILGNKGII 189

Query: 202 PGTRNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMK 261
           PG RNELYPV  SF AP FFS+ERAAAPYFG+K Y + +NGYVE+DG+KFLWI KRS  K
Sbjct: 190 PGIRNELYPVKPSFNAPAFFSIERAAAPYFGLKGYAIHVNGYVERDGQKFLWIGKRSLAK 249

Query: 262 QTFPGMLDELVAGGLPEGVPCGENLMKECEEEAGIPRSISKKAIPVGAVSYSDIKDYSYK 321
            T+PG LD LVAGGLP G+   ENL+KECEEEAGI + ++ +AI VG VSY DI  Y + 
Sbjct: 250 STYPGKLDHLVAGGLPHGISVCENLVKECEEEAGISKVLADRAIAVGVVSYMDIDRYCFT 309

Query: 322 RDVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRH 381
           RDVLFCYDL+LP+ F+P NQDGEV+ FRL+PV  VANV+R+T  FK +C+LVIIDFLFRH
Sbjct: 310 RDVLFCYDLELPQDFVPTNQDGEVDSFRLIPVAQVANVVRKTSFFKDSCSLVIIDFLFRH 364

Query: 382 GYIKPESLGYLELLQSLKSGTC 401
           G I+PES GYL+L + L++G C
Sbjct: 370 GLIRPESPGYLDLYRRLRNGDC 364

[Close]

BLAST of Spg021759 vs. ExPASy Swiss-Prot
Match: P47173 (Uncharacterized protein YJR142W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YJR142W PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.3e-32
Identity = 90/212 (42.45%), Postives = 120/212 (56.60%), Query Frame = 0


Query: 198 IPGTRNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYV--EKDGKKFLWIAKRS 257
           + G RNE Y V        +  +ERA A   GI  YG+ +NGYV   K  K   W+ +RS
Sbjct: 123 VKGWRNEKYAV--WVNKKPYVLVERAVAGVLGIITYGIHINGYVLDPKSKKVQFWVPRRS 182

Query: 258 QMKQTFPGMLDELVAGGLPEGVPCG--ENLMKECEEEAGIPRSISKKAI-PVGAVSY--- 317
           + KQT+P MLD ++AGGL  G P G  E ++KE  EEA + +S+ +  I   G+VSY   
Sbjct: 183 KTKQTWPLMLDNIIAGGL--GYPYGIYETVLKESMEEANLEKSVIEDNIKATGSVSYLYF 242

Query: 318 -SDIKDYSYKR-------DVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIRRTQ 377
             DI    + +       +V + YDLKL E  IP   DGEVE F L  +    N +R+ +
Sbjct: 243 TGDISVTKFNKESDFIVGEVQYVYDLKLSEDIIPKPNDGEVESFNLFSLQETINALRKKE 302

Query: 378 LFKHNCALVIIDFLFRHGYIKPES-LGYLELL 393
            FK NCALV++DFL RHGYI PE+   YLEL+
Sbjct: 303 -FKPNCALVMVDFLIRHGYITPENEPNYLELV 329

[Close]

BLAST of Spg021759 vs. ExPASy Swiss-Prot
Match: P41888 (Thiamine pyrophosphokinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tnr3 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.4e-26
Identity = 76/197 (38.58%), Postives = 107/197 (54.31%), Query Frame = 0


Query: 202 RNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYV--EKDGKKFLWIAKRSQMKQ 261
           RNELY V      PV  ++ER     FG    GV    Y+   K+    +W+ +RS  KQ
Sbjct: 105 RNELYTVYGKSKKPV-LAVERGGFWLFGFLSTGVHCTMYIPATKEHPLRIWVPRRSPTKQ 164

Query: 262 TFPGMLDELVAGGLPEGVPCGENLMKECEEEAGIPRSISKKAIPVGAVSYSDI-KDYSYK 321
           T+P  LD  VAGG+  G      ++KE  EEA +  S S   IP G VSY  + K +  +
Sbjct: 165 TWPNYLDNSVAGGIAHGDSVIGTMIKEFSEEANLDVS-SMNLIPCGTVSYIKMEKRHWIQ 224

Query: 322 RDVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRH 381
            ++ + +DL + +  IP   DGEV GF LLP+  V + +   + FK NCALV++DFL RH
Sbjct: 225 PELQYVFDLPVDDLVIPRINDGEVAGFSLLPLNQVLHEL-ELKSFKPNCALVLLDFLIRH 284

Query: 382 GYIKPESLGYLELLQSL 396
           G I P+   YL+ L+ +
Sbjct: 285 GIITPQHPQYLQTLERI 298

[Close]

BLAST of Spg021759 vs. ExPASy TrEMBL
Match: A0A6J1F0U6 (nudix hydrolase 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111441109 PE=4 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.2e-190
Identity = 342/403 (84.86%), Postives = 352/403 (87.34%), Query Frame = 0


Query: 1   MATSPPIQP--SKFLASHRFGNSPAISSICNASMFLRTRYPTTSISRSTVHRSMTFTSRS 60
           MAT P IQP   K LASHRF NS  ISSI NASMF R RYPT     ST+HRS TF  RS
Sbjct: 1   MATCPRIQPMACKSLASHRFRNSSPISSISNASMFSRARYPTA----STLHRSKTFACRS 60

Query: 61  ISLASSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSVGLFLLFLFVWLCKVYI 120
           +S+ASS+FTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGS                 
Sbjct: 61  VSVASSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGS----------------- 120

Query: 121 SAVIDMQYEFLPFVIDDQIVGYVHHGFANHLKKYANVFTFPRDDSVKFGTCLSLHESLKT 180
               DMQ EFLPFVIDDQIVG+VHHGFA HLKKYANVFTFPRDDSV+ G CLSLHESLKT
Sbjct: 121 ----DMQSEFLPFVIDDQIVGHVHHGFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKT 180

Query: 181 PEDRTHAVGDVVKCLGEEVIPGTRNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMN 240
           PEDRTHAVG+VVKCLGEE+IPG RNE+YPV+SSFGAPVFFSLERAAA YFGIKVYGVQMN
Sbjct: 181 PEDRTHAVGNVVKCLGEEIIPGIRNEMYPVTSSFGAPVFFSLERAAATYFGIKVYGVQMN 240

Query: 241 GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGVPCGENLMKECEEEAGIPRSIS 300
           GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGV CGENLMKECEEEAGIPRSIS
Sbjct: 241 GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGVSCGENLMKECEEEAGIPRSIS 300

Query: 301 KKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIR 360
            KAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIP NQDGEVEGFRLLPVTNVANVIR
Sbjct: 301 NKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIR 360

Query: 361 RTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQSLKSGTCF 402
           RTQLFKHNCALVIIDFLFRHGYIKPES GYLELLQSLKSGTCF
Sbjct: 361 RTQLFKHNCALVIIDFLFRHGYIKPESSGYLELLQSLKSGTCF 378

[Close]

BLAST of Spg021759 vs. ExPASy TrEMBL
Match: A0A6J1KL70 (nudix hydrolase 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111496248 PE=4 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.6e-190
Identity = 340/403 (84.37%), Postives = 351/403 (87.10%), Query Frame = 0


Query: 1   MATSPPIQP--SKFLASHRFGNSPAISSICNASMFLRTRYPTTSISRSTVHRSMTFTSRS 60
           MAT P IQP   K LASH F NS  ISSI NASMF R  YPT     STVHRS TF  RS
Sbjct: 1   MATCPRIQPMACKSLASHPFRNSSPISSISNASMFSRATYPTA----STVHRSKTFACRS 60

Query: 61  ISLASSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSVGLFLLFLFVWLCKVYI 120
           +S+ASS+FTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGS                 
Sbjct: 61  VSIASSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGS----------------- 120

Query: 121 SAVIDMQYEFLPFVIDDQIVGYVHHGFANHLKKYANVFTFPRDDSVKFGTCLSLHESLKT 180
               DMQ EFLPFVIDDQIVG+VHHGFA HLKKYANVFTFPRDDSV+ G CLSLHESLKT
Sbjct: 121 ----DMQSEFLPFVIDDQIVGHVHHGFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKT 180

Query: 181 PEDRTHAVGDVVKCLGEEVIPGTRNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMN 240
           PEDRTHAVG+VVKCLGEE+IPG RNE+YPV+SSFGAPVFFSLERAAAPYFGIKVYGVQMN
Sbjct: 181 PEDRTHAVGNVVKCLGEEIIPGVRNEMYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMN 240

Query: 241 GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGVPCGENLMKECEEEAGIPRSIS 300
           GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEG+ CGENLMKECEEEAGIPRSIS
Sbjct: 241 GYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGISCGENLMKECEEEAGIPRSIS 300

Query: 301 KKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIR 360
            KAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIP NQDGEVEGFRLLPVTNVANVIR
Sbjct: 301 NKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIR 360

Query: 361 RTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQSLKSGTCF 402
           RTQLFKHNCALVII+FLFRHGYIKPE LGYLELLQSLKSGTCF
Sbjct: 361 RTQLFKHNCALVIIEFLFRHGYIKPECLGYLELLQSLKSGTCF 378

[Close]

BLAST of Spg021759 vs. ExPASy TrEMBL
Match: A0A6J1CUZ7 (nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111014513 PE=4 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 7.8e-185
Identity = 327/391 (83.63%), Postives = 345/391 (88.24%), Query Frame = 0


Query: 11  KFLASHRFGNSPAISSICNASMFLRTRYPTTSISRSTVHRSMTFTSRSISLASSSFTWED 70
           K LA HRF N PAIS IC+ASMFLRTR+PTTS  R TVHRS+TF S S+S+ASSSFTWED
Sbjct: 4   KSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSVSVASSSFTWED 63

Query: 71  AVHISLPESFQDDPSDLTGYFEKVKLCNRGSVGLFLLFLFVWLCKVYISAVIDMQYEFLP 130
           A+HISLPESFQDDPSDLTGYFEKVKLCNRGS                     DMQ EFLP
Sbjct: 64  ALHISLPESFQDDPSDLTGYFEKVKLCNRGS---------------------DMQSEFLP 123

Query: 131 FVIDDQIVGYVHHGFANHLKKYANVFTFPRDDSVKFGTCLSLHESLKTPEDRTHAVGDVV 190
           FVID+QI+GYVHHGFA HLKKYANVFTFP+DDSV+ G  L+L+ESL TPEDRT  VGDVV
Sbjct: 124 FVIDNQIIGYVHHGFAEHLKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVV 183

Query: 191 KCLGEEVIPGTRNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLW 250
           KCLGEEVIPGTRNELYPV+SSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVE+DG+KFLW
Sbjct: 184 KCLGEEVIPGTRNELYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVERDGQKFLW 243

Query: 251 IAKRSQMKQTFPGMLDELVAGGLPEGVPCGENLMKECEEEAGIPRSISKKAIPVGAVSYS 310
           IAKRSQMKQTFPGMLDELVAGGLP+GVPCGENL KECEEEAGIPRSIS KAI VGAVSYS
Sbjct: 244 IAKRSQMKQTFPGMLDELVAGGLPQGVPCGENLRKECEEEAGIPRSISNKAISVGAVSYS 303

Query: 311 DIKDYSYKRDVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALV 370
           DIKDYSYKRDVLFCYDLKLPESF+P NQDGEVEGFRLLPV NVANVIRRTQLFK NC+LV
Sbjct: 304 DIKDYSYKRDVLFCYDLKLPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLV 363

Query: 371 IIDFLFRHGYIKPESLGYLELLQSLKSGTCF 402
           IIDFLFRHG IKPESLGYLELLQSLKSGTCF
Sbjct: 364 IIDFLFRHGCIKPESLGYLELLQSLKSGTCF 373

[Close]

BLAST of Spg021759 vs. ExPASy TrEMBL
Match: A0A5A7TT66 (Nudix hydrolase 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G001740 PE=4 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 5.8e-172
Identity = 303/394 (76.90%), Postives = 332/394 (84.26%), Query Frame = 0


Query: 10  SKFLASHRFGNSPAISSICNASMFLRTRYPTTSISRSTVHRSMTFTSRSISLASSSFTWE 69
           SK LA H F N   + S CNAS   RT Y T S  R TVHRS++  SR +S+   SFTW+
Sbjct: 3   SKSLA-HSFRNFSTVPSFCNASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTWD 62

Query: 70  DAVHISLPESFQDDPSDLTGYFEKVKLCNRGSVGLFLLFL--FVWLCKVYISAVIDMQYE 129
           D VH+S PESFQDDP+DLTGYFEKVKLCNRGSVGLFLLF   F    K   + + D Q E
Sbjct: 63  DVVHVSSPESFQDDPADLTGYFEKVKLCNRGSVGLFLLFFSCFAVQTKDVKAFLSDTQSE 122

Query: 130 FLPFVIDDQIVGYVHHGFANHLKKYANVFTFPRDDSVKFGTCLSLHESLKTPEDRTHAVG 189
           F PFVID QIVGYVHHGFA HL++Y NVFTF +DDSVK G CL+LHESLKTPEDRT AVG
Sbjct: 123 FFPFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSVKVGACLTLHESLKTPEDRTRAVG 182

Query: 190 DVVKCLGEEVIPGTRNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKK 249
           DVVKCLGEEVIPGTRNEL+PV+SSFGAP FFSLERAAAPYFGIKVYGV MNGYVEK+GKK
Sbjct: 183 DVVKCLGEEVIPGTRNELFPVTSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKK 242

Query: 250 FLWIAKRSQMKQTFPGMLDELVAGGLPEGVPCGENLMKECEEEAGIPRSISKKAIPVGAV 309
           FLW+AKRSQ K TFPGMLD LVAGGLP+G+ CGENLMKEC+EEAGIPRS+SK+AIPVGA+
Sbjct: 243 FLWVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEEAGIPRSVSKEAIPVGAI 302

Query: 310 SYSDIKDYSYKRDVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIRRTQLFKHNC 369
           SY+DIK Y YKRDV FCYDLKLPESF+P NQDGEVEGF LLPVTNVANVIRRTQ FK NC
Sbjct: 303 SYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNC 362

Query: 370 ALVIIDFLFRHGYIKPESLGYLELLQSLKSGTCF 402
           +LVII+FLFRHGYIKPES GYLELLQSL+SGTCF
Sbjct: 363 SLVIINFLFRHGYIKPESSGYLELLQSLRSGTCF 395

[Close]

BLAST of Spg021759 vs. ExPASy TrEMBL
Match: A0A1S3CJ09 (nudix hydrolase 20, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501537 PE=4 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 2.5e-167
Identity = 294/392 (75.00%), Postives = 321/392 (81.89%), Query Frame = 0


Query: 10  SKFLASHRFGNSPAISSICNASMFLRTRYPTTSISRSTVHRSMTFTSRSISLASSSFTWE 69
           SK LA H F N   + S CNAS   RT Y T S  R TVHRS++  SR +S+   SFTW+
Sbjct: 3   SKSLA-HSFRNFSTVPSFCNASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTWD 62

Query: 70  DAVHISLPESFQDDPSDLTGYFEKVKLCNRGSVGLFLLFLFVWLCKVYISAVIDMQYEFL 129
           D VH+S PESFQDDP+DLTGYFEKVKLCNRGS                     D Q EF 
Sbjct: 63  DVVHVSSPESFQDDPADLTGYFEKVKLCNRGS---------------------DTQSEFF 122

Query: 130 PFVIDDQIVGYVHHGFANHLKKYANVFTFPRDDSVKFGTCLSLHESLKTPEDRTHAVGDV 189
           PFVID QIVGYVHHGFA HL++Y NVFTF +DDSVK G CL+LHESLKTPEDRT AVGDV
Sbjct: 123 PFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSVKVGACLTLHESLKTPEDRTRAVGDV 182

Query: 190 VKCLGEEVIPGTRNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFL 249
           VKCLGEEVIPGTRNEL+PV+SSFGAP FFSLERAAAPYFGIKVYGV MNGYVEK+GKKFL
Sbjct: 183 VKCLGEEVIPGTRNELFPVTSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFL 242

Query: 250 WIAKRSQMKQTFPGMLDELVAGGLPEGVPCGENLMKECEEEAGIPRSISKKAIPVGAVSY 309
           W+AKRSQ K TFPGMLD LVAGGLP+G+ CGENLMKEC+EEAGIPRS+SK+AIPVGA+SY
Sbjct: 243 WVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEEAGIPRSVSKEAIPVGAISY 302

Query: 310 SDIKDYSYKRDVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCAL 369
           +DIK Y YKRDV FCYDLKLPESF+P NQDGEVEGF LLPVTNVANVIRRTQ FK NC+L
Sbjct: 303 TDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSL 362

Query: 370 VIIDFLFRHGYIKPESLGYLELLQSLKSGTCF 402
           VII+FLFRHGYIKPES GYLELLQSL+SGTCF
Sbjct: 363 VIINFLFRHGYIKPESSGYLELLQSLRSGTCF 372

[Close]

BLAST of Spg021759 vs. TAIR 10
Match: AT5G19460.1 (nudix hydrolase homolog 20 )

HSP 1 Score: 406.0 bits (1042), Expect = 3.5e-113
Identity = 205/367 (55.86%), Postives = 261/367 (71.12%), Query Frame = 0


Query: 37  RYPTTSISRSTVHRSMTFTSRSISLASSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKL 96
           R+ ++S+S S +  S   ++ +    SSSFTW+D +     E    + SDLTG+FEK+  
Sbjct: 31  RWRSSSMSLSPLRHSRALSAATTVPISSSFTWDDVIETGRAEY---NSSDLTGFFEKINR 90

Query: 97  CNRGSVGLFLLFLFVWLCKVYISAVIDMQYEFLPFVIDDQIVGYVHHGFANHLKKYANVF 156
           CNRGS  L                      EF+PFVI++QIVGY+H  F  +L+++ ++F
Sbjct: 91  CNRGSEKL---------------------GEFIPFVIEEQIVGYIHKRFTEYLREFHDIF 150

Query: 157 TFPRDDSV--KFGTCLSLHESLKTPEDRTHAVGDVVKCLGEE-VIPGTRNELYPVSSSFG 216
           TF ++ S   +    ++L+  L+ PEDRT AV DV+K LG++ +IPG RNELYPV  SF 
Sbjct: 151 TFSQNGSCPDRVDGYVTLNLMLQKPEDRTRAVADVIKILGDKGIIPGIRNELYPVKPSFN 210

Query: 217 APVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGL 276
           APVFFSLERAAAPYFGIK YGV MNGYVE+DG+K LWI KRS  K T+PGMLD LVAGGL
Sbjct: 211 APVFFSLERAAAPYFGIKGYGVHMNGYVERDGQKLLWIGKRSLSKSTYPGMLDHLVAGGL 270

Query: 277 PEGVPCGENLMKECEEEAGIPRSISKKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESF 336
           P G+ CG NL+KECEEEAGI R+I+ +AI VGAVSY DI  Y +KRDVLFCYDL+LPE F
Sbjct: 271 PHGISCGGNLVKECEEEAGISRAIADRAIAVGAVSYLDIDQYCFKRDVLFCYDLELPEDF 330

Query: 337 IPMNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQ 396
           +P NQDGEVE F+L+PV  VA+VI++T  FK NC+LVIIDFLFRHG+I+PES GYL+L Q
Sbjct: 331 VPKNQDGEVESFKLIPVAQVASVIKKTSFFKANCSLVIIDFLFRHGFIRPESSGYLDLYQ 373

Query: 397 SLKSGTC 401
            L++  C
Sbjct: 391 RLRNRDC 373

[Close]

BLAST of Spg021759 vs. TAIR 10
Match: AT5G19470.1 (nudix hydrolase homolog 24 )

HSP 1 Score: 378.3 bits (970), Expect = 7.9e-105
Identity = 195/382 (51.05%), Postives = 258/382 (67.54%), Query Frame = 0


Query: 22  PAISSICNASMFLRT-RYPTTSISRS-TVHRSMTFTSRSISLASSSFTWEDAVHISLPES 81
           P  +S+ ++   + T ++ ++S SRS  +H S   +  ++ L S SFTW D    S  E 
Sbjct: 10  PTPTSLFSSHALIPTLQWRSSSSSRSPPLHISRVLSVETVPL-SPSFTWNDVFENSRKEY 69

Query: 82  FQDDPSDLTGYFEKVKLCNRGSVGLFLLFLFVWLCKVYISAVIDMQYEFLPFVIDDQIVG 141
              + SDLTG+ EKV  CNRG                     ++   EF+PFVI++QIVG
Sbjct: 70  VPQNSSDLTGFLEKVDRCNRG---------------------LEKLGEFIPFVIEEQIVG 129

Query: 142 YVHHGFANHLKKYANVFTFPRDDSVKFGTCLSLHESLKTPEDRTHAVGDVVKCLGEE-VI 201
           Y+H GF  +L+ + ++FTF      ++G  ++L+  L  PE+RT AV  V+K LG + +I
Sbjct: 130 YIHKGFTKYLRDFNDIFTFS-----QYGGHVTLNMMLDKPEERTRAVAHVIKILGNKGII 189

Query: 202 PGTRNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMK 261
           PG RNELYPV  SF AP FFS+ERAAAPYFG+K Y + +NGYVE+DG+KFLWI KRS  K
Sbjct: 190 PGIRNELYPVKPSFNAPAFFSIERAAAPYFGLKGYAIHVNGYVERDGQKFLWIGKRSLAK 249

Query: 262 QTFPGMLDELVAGGLPEGVPCGENLMKECEEEAGIPRSISKKAIPVGAVSYSDIKDYSYK 321
            T+PG LD LVAGGLP G+   ENL+KECEEEAGI + ++ +AI VG VSY DI  Y + 
Sbjct: 250 STYPGKLDHLVAGGLPHGISVCENLVKECEEEAGISKVLADRAIAVGVVSYMDIDRYCFT 309

Query: 322 RDVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRH 381
           RDVLFCYDL+LP+ F+P NQDGEV+ FRL+PV  VANV+R+T  FK +C+LVIIDFLFRH
Sbjct: 310 RDVLFCYDLELPQDFVPTNQDGEVDSFRLIPVAQVANVVRKTSFFKDSCSLVIIDFLFRH 364

Query: 382 GYIKPESLGYLELLQSLKSGTC 401
           G I+PES GYL+L + L++G C
Sbjct: 370 GLIRPESPGYLDLYRRLRNGDC 364

[Close]

BLAST of Spg021759 vs. TAIR 10
Match: AT5G19470.2 (nudix hydrolase homolog 24 )

HSP 1 Score: 365.2 bits (936), Expect = 6.9e-101
Identity = 192/382 (50.26%), Postives = 254/382 (66.49%), Query Frame = 0


Query: 22  PAISSICNASMFLRT-RYPTTSISRS-TVHRSMTFTSRSISLASSSFTWEDAVHISLPES 81
           P  +S+ ++   + T ++ ++S SRS  +H S   +  ++ L S SFTW D    S  E 
Sbjct: 10  PTPTSLFSSHALIPTLQWRSSSSSRSPPLHISRVLSVETVPL-SPSFTWNDVFENSRKEY 69

Query: 82  FQDDPSDLTGYFEKVKLCNRGSVGLFLLFLFVWLCKVYISAVIDMQYEFLPFVIDDQIVG 141
              + SDLTG+ EKV  CNRG                     ++   EF+PFVI++QIVG
Sbjct: 70  VPQNSSDLTGFLEKVDRCNRG---------------------LEKLGEFIPFVIEEQIVG 129

Query: 142 YVHHGFANHLKKYANVFTFPRDDSVKFGTCLSLHESLKTPEDRTHAVGDVVKCLGEE-VI 201
           Y+H GF  +L+ + ++FTF      ++G  ++L+  L  PE+RT AV  V+K LG + +I
Sbjct: 130 YIHKGFTKYLRDFNDIFTFS-----QYGGHVTLNMMLDKPEERTRAVAHVIKILGNKGII 189

Query: 202 PGTRNELYPVSSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMK 261
           PG RNELYPV  SF AP FFS+ERAAAPYFG+K Y + +NGYVE+DG+KFLWI KRS  K
Sbjct: 190 PGIRNELYPVKPSFNAPAFFSIERAAAPYFGLKGYAIHVNGYVERDGQKFLWIGKRSLAK 249

Query: 262 QTFPGMLDELVAGGLPEGVPCGENLMKECEEEAGIPRSISKKAIPVGAVSYSDIKDYSYK 321
            T+PG LD LVAGGL        NL+KECEEEAGI + ++ +AI VG VSY DI  Y + 
Sbjct: 250 STYPGKLDHLVAGGL--------NLVKECEEEAGISKVLADRAIAVGVVSYMDIDRYCFT 309

Query: 322 RDVLFCYDLKLPESFIPMNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRH 381
           RDVLFCYDL+LP+ F+P NQDGEV+ FRL+PV  VANV+R+T  FK +C+LVIIDFLFRH
Sbjct: 310 RDVLFCYDLELPQDFVPTNQDGEVDSFRLIPVAQVANVVRKTSFFKDSCSLVIIDFLFRH 356

Query: 382 GYIKPESLGYLELLQSLKSGTC 401
           G I+PES GYL+L + L++G C
Sbjct: 370 GLIRPESPGYLDLYRRLRNGDC 356

The following BLAST results are available for this feature:

BLAST of Spg021759 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023530783.1	6.8e-191	85.11	nudix hydrolase 20, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_02353078...	[more]
XP_022933802.1	2.6e-190	84.86	nudix hydrolase 20, chloroplastic-like [Cucurbita moschata]	[more]
XP_023002396.1	7.5e-190	84.37	nudix hydrolase 20, chloroplastic-like [Cucurbita maxima]	[more]
KAG6587948.1	1.3e-189	84.86	Nudix hydrolase 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038878620.1	4.5e-187	83.37	nudix hydrolase 20, chloroplastic-like [Benincasa hispida]	[more]

BLAST of Spg021759 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VXZ0	5.0e-112	55.86	Nudix hydrolase 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT20 PE=2...	[more]
P0C026	1.1e-103	51.05	Nudix hydrolase 24, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT24 PE=2...	[more]
P47173	2.3e-32	42.45	Uncharacterized protein YJR142W OS=Saccharomyces cerevisiae (strain ATCC 204508 ...	[more]
P41888	1.4e-26	38.58	Thiamine pyrophosphokinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843...	[more]

BLAST of Spg021759 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F0U6	1.2e-190	84.86	nudix hydrolase 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1KL70	3.6e-190	84.37	nudix hydrolase 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114962...	[more]
A0A6J1CUZ7	7.8e-185	83.63	nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A5A7TT66	5.8e-172	76.90	Nudix hydrolase 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G0...	[more]
A0A1S3CJ09	2.5e-167	75.00	nudix hydrolase 20, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC...	[more]

BLAST of Spg021759 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19460.1	3.5e-113	55.86	nudix hydrolase homolog 20	[more]
AT5G19470.1	7.9e-105	51.05	nudix hydrolase homolog 24	[more]
AT5G19470.2	6.9e-101	50.26	nudix hydrolase homolog 24	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000086	NUDIX hydrolase domain	PFAM	PF00293	NUDIX	coord: 244..353 e-value: 2.0E-7 score: 31.1
IPR000086	NUDIX hydrolase domain	PROSITE	PS51462	NUDIX	coord: 232..373 score: 10.184038
None	No IPR available	GENE3D	3.90.79.10	Nucleoside Triphosphate Pyrophosphohydrolase	coord: 217..397 e-value: 1.1E-65 score: 222.8
None	No IPR available	PANTHER	PTHR13622	THIAMIN PYROPHOSPHOKINASE	coord: 64..398
None	No IPR available	PANTHER	PTHR13622:SF10	SI:DKEY-6N6.2	coord: 64..398
None	No IPR available	CDD	cd03676	Nudix_hydrolase_3	coord: 200..379 e-value: 9.82445E-78 score: 236.392
IPR031804	Domain of unknown function DUF4743	PFAM	PF15916	DUF4743	coord: 125..231 e-value: 1.5E-32 score: 112.1
IPR015797	NUDIX hydrolase-like domain superfamily	SUPERFAMILY	55811	Nudix	coord: 204..377

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg021759.1	Spg021759.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0044715	8-oxo-dGDP phosphatase activity