Spg021525 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg021525
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionTMV resistance protein N-like
Locationscaffold9: 7608156 .. 7611459 (-)
RNA-Seq ExpressionSpg021525
SyntenySpg021525
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGTCGTAATCTTAAGCTCTTTCCACCAAAATCTCCAAACTTTGGCTCTTATGAGTACAGGTCTTGAAAGTTCTCCAAAGATAGGGGGAAATATGGAACATTTGACTCAACTTCATCTTGCTGGATCTAATATCACCCAACTTCATCCTTCAATTGAATGTCTAACGGGCTTGGCATTGTTGAACCTATCTCATTGTACTAGACTTTTGAGTCTTCCTACTGAAATTGGTAACTTGAGGGCTTTGAAAATCCTCATTTTGCAATGTTGTACAAAACTTTACCAAATTCCTCCCAGCTTGGCAAATGCAGAATCCTTGGAGAAACTTAATATTAGTGGCACCTCTGTAAGCCATGTACCAACAAGCATGCATCATCTAAAGAATTTAATAAGGTTATATTGTGAGGACTTTCACGTGGCATTTGGAAGTCATTGGTCCCCCAATTCAAAAGTAAACCAATATCTAGTGGTTTGGGATGCCTTAAAACTCTAAATCTGAGTTGTTGCAGACTTGTAGATGAAGACGTACCCAAAGATCTCCATTGCTTTTCCTCATTAGAAGAACTCAATCTCAGTTATAATGAGTTTATAAAGCTCCCAGATAGCCTCAGTCATCTTAAGAAGTTAAAAAGGTTGTACTTGAATTATTGCAGGAAGCTTAAAGACTTACCAAAGCTTCCTACAAGTTTGGAAAATGTAGTAGCTGTAGGATGCATATCAATATCAGAAAAGTTTTACAATAAAAGATTACATATCTTCTCGGATTTGAAAGAAAATCTTCTCCTTGAATTTTCAATTTCTTCCAAGACTACGAAAGTAGAATGCAACGTTAATGAGCTCCAGTATACGATGTTAGATCGAAGATTGTGTGAGGTTTTTCTCTCCATCCCATAAAACCTATTAAATACCGTCTTTTGGATTAAAAAACAACTTTCTAATTTATCTTATTTTTGCAGGCATCTATTTCAGGTGAGAAACGACGCACATACATATATAGTAATGTTGACACCTTGGAGTGCTTTGACCATATAAACGAAGGGAATTGGATAAACATTGTGTACAACCAAGAATCCTCATTTAACAAAGATATAAGCAATGTTTGTGGATTTTTATTCTCAGCTAATATAGAATATCAACAAACATTGGATCATTTTGAAGATATTATTGGCAACCTTTCAGTTAAGTTCAAGATTGATGGTAATCCACCAAATTTTTATATGTGGTTTAAGAAGAAATTTCAATCTCAAAGAAGCATTTGGGTAGCATACCTTCCGATTAAGCTTCTTAAAGACGAATTTATGGTTCAAAGTTGTTGCTCTGTCAAGATTACAATTAGCTGTGATAACATATATGCAAAGGTCAAAGCATGTGGTGTTTCTATACTCTCTTGGCAAACTTTTTCACTATGCTCTATTGCTCCAAACGCATGTTCCATAGAAGAATTAAGGAACCTAATCATGAGGGTGAATGCAGGTGTGATGAGCTTGAAATCGCAAAGGATGATTTTCCATCTTCTGCATTTGAATCTACTGAAACTGAGTCGACATTTTTGCCAAGGAAAAACCTCAGATCAATACTTGAGAGAATATTTGAGGTTTGTCTTCTTTGAATACGACAATATCTCTATTTAACTTAGCTCACTCTTTAATTTCTAAACATAATAATATATAGTATTTAATAACTAGTAAACAAAAATTTAAAGGATTTTTTTTTAACCATGTTTCTAGTAACCCAATAATACTCGTTTCACATATCTTACAGGAAGACAAGAGTTGTTTCCAAGTAAGCTACTTCCCACAAACAAAAGTTTCTAGATGGTTTAGAAATCAAAATAAGAAAGACAAAGTAGCAATAAAGCTTCCTCTAAATATACGTAAAGATAAGAAGTGGATGGGGTTGGCAGCGTTTGGTATCTTCTCAATCTCTCAGAATGCTAATTGTTATTGCTTAAGTTATGAAGTACAATTAGATGATACGTGTCTATATCGTGACCATATCCCCATGAAGTTGTTAGAGTATTCAAATCGAATACTGATTCTTGTGTTTAAACCAAGTGATTGTTATACATATGAACGAAACTCTTCATCCAACCATGTTTATATTAGTTTCAATACTAATGTTGAAAGAATGCAAGTTGAGTCATGTGGAGCTCGACTAGTTTATAAGGATAATGTTAAGGGACTTATTCAGACAATTGTGGATTGCATCGTGGAGAGTGAAGATGGCCTTTATGAATATTACAACCAAATACTTATGGCAGATCTCTTCAGCATGATTAATTCATCTAAGCATAAAGTGTCGTCCCTTGAAAGTGCCTCTGTCAACAAACCCGCTACTACAACTACTGCTTTGACCGATACTGATTCAAGCCTTTTAGTTGAGCAACTTTTATATGGAAGTTTCCCGCACCCACTCCTCCAAAAGAATATCAAGGTTATCTGATTTATCTATCTTTTTCTTTATTTCCAACAAATTCCATATTATTGCAGGAACGATTCGAAAGTAAGTTTGATCTTATTCTACATGGAGATAAGATCCCAGAATTTTTCAACAATAAAAGTACAGGAAACATGACGAGCTTAAAGTTACCACAAGATCTACATCCACACAAAGTAGGTCTTATTGTGTGCGCTCTTGTGGTTCTGGACAAGCAAACGGGAAAGCTAAATGACATTCAAGAGCGAGGGAGGTATACAAAAGTTGTAGATCTTATATGCAAACTGAAAGTTGACTCATATGAAATTAAGCTAGTGCATTACTACTTTGTGCCTCAAGAGAATCTCTTGAGTCGATATGCATCACACTTCATTTGGCTCTTTCACACGCCTCTTATTGATTTTGATATTTCATGGCACAATTCCAACCACATTGGAATGACACTAGAGACTAGCAGCCATGATGATCTATTTTTGGTGCAACATTGTGGTCTTCATCTGATACGCGAGGGAGAAAGAAAGATGATTGATAAGATGATAATGGAGTCAACTGTTCCACCATCCATTGGTGGTGCCATTTGACAAAACTTGAGAAACAACTCTGAACTTTCTATTTTTGAAGGCGTTCATAAATGTTGTAGTATTTGGATTTGAATATGTTTGGCCCATTTTGATGTATTGTATTGGGCTCTTTGCTGATAAATCTTATTGGTTTAGTCTGGTCGTTAAATCGGTTGAAGGTTAACTGTAGATCTATGTCTCACTGTTCGGCTCTGCTTTCTCACTTCCTTAGTAGGTACACACTGTATAAGGAATTTTGATATTGGTTTTATGTGAGAATGCTCATTTATGTAATTCTTCTTCTTA

mRNA sequence

ATGGTTGTCGTAATCTTAAGCTCTTTCCACCAAAATCTCCAAACTTTGGCTCTTATGAGTACAGGTCTTGAAAGTTCTCCAAAGATAGGGGGAAATATGGAACATTTGACTCAACTTCATCTTGCTGGATCTAATATCACCCAACTTCATCCTTCAATTGAATGTCTAACGGGCTTGGCATTGTTGAACCTATCTCATTGTACTAGACTTTTGAGTCTTCCTACTGAAATTGGTAACTTGAGGGCTTTGAAAATCCTCATTTTGCAATGTTGTACAAAACTTTACCAAATTCCTCCCAGCTTGGCAAATGCAGAATCCTTGGAGAAACTTAATATTAGTGGCACCTCTGTAAGCCATGTACCAACAAGCATGCATCATCTAAAGAATTTAATAAGACTTGTAGATGAAGACGTACCCAAAGATCTCCATTGCTTTTCCTCATTAGAAGAACTCAATCTCAGTTATAATGAGTTTATAAAGCTCCCAGATAGCCTCAGTCATCTTAAGAAGTTAAAAAGGTTGTACTTGAATTATTGCAGGAAGCTTAAAGACTTACCAAAGCTTCCTACAAGTTTGGAAAATGTAGTAGCTGTAGGATGCATATCAATATCAGAAAAGTTTTACAATAAAAGATTACATATCTTCTCGGATTTGAAAGAAAATCTTCTCCTTGAATTTTCAATTTCTTCCAAGACTACGAAAGTAGAATGCAACGTTAATGAGCTCCAGTATACGATGTTAGATCGAAGATTGTGCATCTATTTCAGGTGTGATGAGCTTGAAATCGCAAAGGATGATTTTCCATCTTCTGCATTTGAATCTACTGAAACTGAGTCGACATTTTTGCCAAGGAAAAACCTCAGATCAATACTTGAGAGAATATTTGAGGAAGACAAGAGTTGTTTCCAAGTAAGCTACTTCCCACAAACAAAAGTTTCTAGATGGTTTAGAAATCAAAATAAGAAAGACAAAGTAGCAATAAAGCTTCCTCTAAATATACGTAAAGATAAGAAGTGGATGGGGTTGGCAGCGTTTGGTATCTTCTCAATCTCTCAGAATGCTAATTGTTATTGCTTAAGTTATGAAGTACAATTAGATGATACGTGTCTATATCGTGACCATATCCCCATGAAGTTGTTAGAGTATTCAAATCGAATACTGATTCTTGTGTTTAAACCAAGTGATTGTTATACATATGAACGAAACTCTTCATCCAACCATGTTTATATTAGTTTCAATACTAATGTTGAAAGAATGCAAGTTGAGTCATGTGGAGCTCGACTAGTTTATAAGGATAATGTTAAGGGACTTATTCAGACAATTGTGGATTGCATCGTGGAGAGTGAAGATGGCCTTTATGAATATTACAACCAAATACTTATGGCAGATCTCTTCAGCATGATTAATTCATCTAAGCATAAAGTGTCGTCCCTTGAAAGTGCCTCTGTCAACAAACCCGCTACTACAACTACTGCTTTGACCGATACTGATTCAAGCCTTTTAGTTGAGCAACTTTTATATGGAAGTTTCCCGCACCCACTCCTCCAAAAGAATATCAAGGAACGATTCGAAAGTAAGTTTGATCTTATTCTACATGGAGATAAGATCCCAGAATTTTTCAACAATAAAAGTACAGGAAACATGACGAGCTTAAAGTTACCACAAGATCTACATCCACACAAAGTAGGTCTTATTGTGTGCGCTCTTGTGGTTCTGGACAAGCAAACGGGAAAGCTAAATGACATTCAAGAGCGAGGGAGGTATACAAAAGTTGTAGATCTTATATGCAAACTGAAAGTTGACTCATATGAAATTAAGCTAGTGCATTACTACTTTGTGCCTCAAGAGAATCTCTTGAGTCGATATGCATCACACTTCATTTGGCTCTTTCACACGCCTCTTATTGATTTTGATATTTCATGGCACAATTCCAACCACATTGGAATGACACTAGAGACTAGCAGCCATGATGATCTATTTTTGGTGCAACATTGTGGTCTTCATCTGATACGCGAGGGAGAAAGAAAGATGATTGATAAGATGATAATGGAGTCAACTGTTCCACCATCCATTGGTGGTGCCATTTGA

Coding sequence (CDS)

ATGGTTGTCGTAATCTTAAGCTCTTTCCACCAAAATCTCCAAACTTTGGCTCTTATGAGTACAGGTCTTGAAAGTTCTCCAAAGATAGGGGGAAATATGGAACATTTGACTCAACTTCATCTTGCTGGATCTAATATCACCCAACTTCATCCTTCAATTGAATGTCTAACGGGCTTGGCATTGTTGAACCTATCTCATTGTACTAGACTTTTGAGTCTTCCTACTGAAATTGGTAACTTGAGGGCTTTGAAAATCCTCATTTTGCAATGTTGTACAAAACTTTACCAAATTCCTCCCAGCTTGGCAAATGCAGAATCCTTGGAGAAACTTAATATTAGTGGCACCTCTGTAAGCCATGTACCAACAAGCATGCATCATCTAAAGAATTTAATAAGACTTGTAGATGAAGACGTACCCAAAGATCTCCATTGCTTTTCCTCATTAGAAGAACTCAATCTCAGTTATAATGAGTTTATAAAGCTCCCAGATAGCCTCAGTCATCTTAAGAAGTTAAAAAGGTTGTACTTGAATTATTGCAGGAAGCTTAAAGACTTACCAAAGCTTCCTACAAGTTTGGAAAATGTAGTAGCTGTAGGATGCATATCAATATCAGAAAAGTTTTACAATAAAAGATTACATATCTTCTCGGATTTGAAAGAAAATCTTCTCCTTGAATTTTCAATTTCTTCCAAGACTACGAAAGTAGAATGCAACGTTAATGAGCTCCAGTATACGATGTTAGATCGAAGATTGTGCATCTATTTCAGGTGTGATGAGCTTGAAATCGCAAAGGATGATTTTCCATCTTCTGCATTTGAATCTACTGAAACTGAGTCGACATTTTTGCCAAGGAAAAACCTCAGATCAATACTTGAGAGAATATTTGAGGAAGACAAGAGTTGTTTCCAAGTAAGCTACTTCCCACAAACAAAAGTTTCTAGATGGTTTAGAAATCAAAATAAGAAAGACAAAGTAGCAATAAAGCTTCCTCTAAATATACGTAAAGATAAGAAGTGGATGGGGTTGGCAGCGTTTGGTATCTTCTCAATCTCTCAGAATGCTAATTGTTATTGCTTAAGTTATGAAGTACAATTAGATGATACGTGTCTATATCGTGACCATATCCCCATGAAGTTGTTAGAGTATTCAAATCGAATACTGATTCTTGTGTTTAAACCAAGTGATTGTTATACATATGAACGAAACTCTTCATCCAACCATGTTTATATTAGTTTCAATACTAATGTTGAAAGAATGCAAGTTGAGTCATGTGGAGCTCGACTAGTTTATAAGGATAATGTTAAGGGACTTATTCAGACAATTGTGGATTGCATCGTGGAGAGTGAAGATGGCCTTTATGAATATTACAACCAAATACTTATGGCAGATCTCTTCAGCATGATTAATTCATCTAAGCATAAAGTGTCGTCCCTTGAAAGTGCCTCTGTCAACAAACCCGCTACTACAACTACTGCTTTGACCGATACTGATTCAAGCCTTTTAGTTGAGCAACTTTTATATGGAAGTTTCCCGCACCCACTCCTCCAAAAGAATATCAAGGAACGATTCGAAAGTAAGTTTGATCTTATTCTACATGGAGATAAGATCCCAGAATTTTTCAACAATAAAAGTACAGGAAACATGACGAGCTTAAAGTTACCACAAGATCTACATCCACACAAAGTAGGTCTTATTGTGTGCGCTCTTGTGGTTCTGGACAAGCAAACGGGAAAGCTAAATGACATTCAAGAGCGAGGGAGGTATACAAAAGTTGTAGATCTTATATGCAAACTGAAAGTTGACTCATATGAAATTAAGCTAGTGCATTACTACTTTGTGCCTCAAGAGAATCTCTTGAGTCGATATGCATCACACTTCATTTGGCTCTTTCACACGCCTCTTATTGATTTTGATATTTCATGGCACAATTCCAACCACATTGGAATGACACTAGAGACTAGCAGCCATGATGATCTATTTTTGGTGCAACATTGTGGTCTTCATCTGATACGCGAGGGAGAAAGAAAGATGATTGATAAGATGATAATGGAGTCAACTGTTCCACCATCCATTGGTGGTGCCATTTGA

Protein sequence

MVVVILSSFHQNLQTLALMSTGLESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRLLSLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISGTSVSHVPTSMHHLKNLIRLVDEDVPKDLHCFSSLEELNLSYNEFIKLPDSLSHLKKLKRLYLNYCRKLKDLPKLPTSLENVVAVGCISISEKFYNKRLHIFSDLKENLLLEFSISSKTTKVECNVNELQYTMLDRRLCIYFRCDELEIAKDDFPSSAFESTETESTFLPRKNLRSILERIFEEDKSCFQVSYFPQTKVSRWFRNQNKKDKVAIKLPLNIRKDKKWMGLAAFGIFSISQNANCYCLSYEVQLDDTCLYRDHIPMKLLEYSNRILILVFKPSDCYTYERNSSSNHVYISFNTNVERMQVESCGARLVYKDNVKGLIQTIVDCIVESEDGLYEYYNQILMADLFSMINSSKHKVSSLESASVNKPATTTTALTDTDSSLLVEQLLYGSFPHPLLQKNIKERFESKFDLILHGDKIPEFFNNKSTGNMTSLKLPQDLHPHKVGLIVCALVVLDKQTGKLNDIQERGRYTKVVDLICKLKVDSYEIKLVHYYFVPQENLLSRYASHFIWLFHTPLIDFDISWHNSNHIGMTLETSSHDDLFLVQHCGLHLIREGERKMIDKMIMESTVPPSIGGAI
Homology
BLAST of Spg021525 vs. NCBI nr
Match: XP_038890520.1 (TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890521.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890522.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890523.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890524.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890525.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890526.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890527.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890528.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890530.1 TMV resistance protein N-like isoform X1 [Benincasa hispida])

HSP 1 Score: 654.1 bits (1686), Expect = 1.4e-183
Identity = 415/922 (45.01%), Postives = 505/922 (54.77%), Query Frame = 0

Query: 11   QNLQTLALMSTGLESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRL 70
            +NLQTL L  TGLE+SP+IGGNMEHLT LHL  SNITQLHPSI  LTGL LL+LS C  L
Sbjct: 705  KNLQTLKLSGTGLETSPEIGGNMEHLTHLHLDESNITQLHPSIGHLTGLVLLDLSSCLGL 764

Query: 71   LSLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISGTSVSHVPTS-MHHLKN 130
             SLP EIGNL++LK L+L+ C KL QIP SLANAESLE L IS TS++H+P+S +H LKN
Sbjct: 765  SSLPCEIGNLKSLKTLLLKYCEKLDQIPKSLANAESLETLCISETSITHLPSSIIHCLKN 824

Query: 131  L----------------------------------------IRLVDEDVPKDLHCFSSLE 190
            L                                         +L+DED+P+DLH FSSLE
Sbjct: 825  LETLECEGLSCGIWKSMLPQFNIHQTVSTGLGCLKTLNLMGCKLMDEDIPEDLHFFSSLE 884

Query: 191  ELNLSYNEFIKLPDSLSHLKKLKRLYLNYCRKLKDLPKLPTSLENVVAVGCISISEKFYN 250
             L+LSYN F  LP SLSHL KLK L LN+C +LKDLPKLP SL+ V  + C S+SE++YN
Sbjct: 885  TLDLSYNNFTTLPVSLSHLNKLKTLNLNFCTELKDLPKLPDSLQYVGGIDCRSMSEQYYN 944

Query: 251  KRLHIFSDLKENLLLEFSISSKTTKVECNVNELQYTMLDRR------------------- 310
            K L I S     L L F I SK   VEC +NE Q+++  RR                   
Sbjct: 945  KILLIPSSSGHQLYLNFIIRSKDANVECAMNEFQHSIFTRRSFELNIIEEKPSTIIHKDV 1004

Query: 311  ----------------------------------------LCIYF--------------- 370
                                                    +C  F               
Sbjct: 1005 DMLEWFGQINEGNWINIQYEQEFSISKPLNIMYEDVDLSNVCGVFLSTNIEFPQNLDHLA 1064

Query: 371  ------------------------------------------------------------ 430
                                                                        
Sbjct: 1065 LGRFSVSFEIDGKCSGGTMGYEMSQFKATRFFWVAYIPIWMFIHHSLMVQRCCSIKATIS 1124

Query: 431  -----------------------------------------------------------R 490
                                                                       R
Sbjct: 1125 YFCDHIDASKVKIKACGVSSLLSWPNVSEYLAKLFAKRYCSKRNFCTMIRQHNDHNSECR 1184

Query: 491  CDELEIAKDDFPSSAFESTETESTFLPRKNLRSILERIFEEDKSCFQVSYFPQTKVSRWF 550
            CDELE+AKDDF S  FES   +STFL R NLR+IL  +FEE K  +   +FPQTK   WF
Sbjct: 1185 CDELEVAKDDFSSYTFES--DDSTFLLRMNLRAILGIMFEEKKRYYMKYFFPQTKFFGWF 1244

Query: 551  RNQNKKDKVAIKLPLNIRKDKKWMGLAAFGIFSISQNANCYCLSYEVQLDDTCL--YRDH 610
            +NQNKKDKVA+K+P+NI KDKKWMGLA F +FSIS+ A+CYC  YE+Q  +  +   R  
Sbjct: 1245 KNQNKKDKVAVKIPVNIDKDKKWMGLAMFVVFSISKKASCYCFEYEIQTKEKIISTQRHS 1304

Query: 611  IPMKLLEYSNRILILVFKPS-DCYTYE--RNSSSNHVYISFNTNVERMQVESCGARLVYK 670
            IP ++ EYSN+IL +VF+P  + Y Y+  ++SSSNHVYI+FNTN ERM+VE CGARLVY+
Sbjct: 1305 IPKEVAEYSNQILFIVFEPRYNWYPYDELKSSSSNHVYINFNTNSERMRVELCGARLVYQ 1364

Query: 671  DNVKGLIQTIVDCIVESEDGLYEYYNQILMADLFSMINSSKHKVSSLESASVNKPATTTT 691
             NV+GLI TIV+CIV+S D LYEYYNQ ++     MIN+  + +S   +  V K    TT
Sbjct: 1365 QNVEGLIHTIVNCIVKSGDELYEYYNQQIVEYHLKMINTHWYTISLPRNNPV-KNQKPTT 1424

BLAST of Spg021525 vs. NCBI nr
Match: XP_038890531.1 (disease resistance-like protein DSC1 isoform X2 [Benincasa hispida])

HSP 1 Score: 654.1 bits (1686), Expect = 1.4e-183
Identity = 415/922 (45.01%), Postives = 505/922 (54.77%), Query Frame = 0

Query: 11   QNLQTLALMSTGLESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRL 70
            +NLQTL L  TGLE+SP+IGGNMEHLT LHL  SNITQLHPSI  LTGL LL+LS C  L
Sbjct: 387  KNLQTLKLSGTGLETSPEIGGNMEHLTHLHLDESNITQLHPSIGHLTGLVLLDLSSCLGL 446

Query: 71   LSLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISGTSVSHVPTS-MHHLKN 130
             SLP EIGNL++LK L+L+ C KL QIP SLANAESLE L IS TS++H+P+S +H LKN
Sbjct: 447  SSLPCEIGNLKSLKTLLLKYCEKLDQIPKSLANAESLETLCISETSITHLPSSIIHCLKN 506

Query: 131  L----------------------------------------IRLVDEDVPKDLHCFSSLE 190
            L                                         +L+DED+P+DLH FSSLE
Sbjct: 507  LETLECEGLSCGIWKSMLPQFNIHQTVSTGLGCLKTLNLMGCKLMDEDIPEDLHFFSSLE 566

Query: 191  ELNLSYNEFIKLPDSLSHLKKLKRLYLNYCRKLKDLPKLPTSLENVVAVGCISISEKFYN 250
             L+LSYN F  LP SLSHL KLK L LN+C +LKDLPKLP SL+ V  + C S+SE++YN
Sbjct: 567  TLDLSYNNFTTLPVSLSHLNKLKTLNLNFCTELKDLPKLPDSLQYVGGIDCRSMSEQYYN 626

Query: 251  KRLHIFSDLKENLLLEFSISSKTTKVECNVNELQYTMLDRR------------------- 310
            K L I S     L L F I SK   VEC +NE Q+++  RR                   
Sbjct: 627  KILLIPSSSGHQLYLNFIIRSKDANVECAMNEFQHSIFTRRSFELNIIEEKPSTIIHKDV 686

Query: 311  ----------------------------------------LCIYF--------------- 370
                                                    +C  F               
Sbjct: 687  DMLEWFGQINEGNWINIQYEQEFSISKPLNIMYEDVDLSNVCGVFLSTNIEFPQNLDHLA 746

Query: 371  ------------------------------------------------------------ 430
                                                                        
Sbjct: 747  LGRFSVSFEIDGKCSGGTMGYEMSQFKATRFFWVAYIPIWMFIHHSLMVQRCCSIKATIS 806

Query: 431  -----------------------------------------------------------R 490
                                                                       R
Sbjct: 807  YFCDHIDASKVKIKACGVSSLLSWPNVSEYLAKLFAKRYCSKRNFCTMIRQHNDHNSECR 866

Query: 491  CDELEIAKDDFPSSAFESTETESTFLPRKNLRSILERIFEEDKSCFQVSYFPQTKVSRWF 550
            CDELE+AKDDF S  FES   +STFL R NLR+IL  +FEE K  +   +FPQTK   WF
Sbjct: 867  CDELEVAKDDFSSYTFES--DDSTFLLRMNLRAILGIMFEEKKRYYMKYFFPQTKFFGWF 926

Query: 551  RNQNKKDKVAIKLPLNIRKDKKWMGLAAFGIFSISQNANCYCLSYEVQLDDTCL--YRDH 610
            +NQNKKDKVA+K+P+NI KDKKWMGLA F +FSIS+ A+CYC  YE+Q  +  +   R  
Sbjct: 927  KNQNKKDKVAVKIPVNIDKDKKWMGLAMFVVFSISKKASCYCFEYEIQTKEKIISTQRHS 986

Query: 611  IPMKLLEYSNRILILVFKPS-DCYTYE--RNSSSNHVYISFNTNVERMQVESCGARLVYK 670
            IP ++ EYSN+IL +VF+P  + Y Y+  ++SSSNHVYI+FNTN ERM+VE CGARLVY+
Sbjct: 987  IPKEVAEYSNQILFIVFEPRYNWYPYDELKSSSSNHVYINFNTNSERMRVELCGARLVYQ 1046

Query: 671  DNVKGLIQTIVDCIVESEDGLYEYYNQILMADLFSMINSSKHKVSSLESASVNKPATTTT 691
             NV+GLI TIV+CIV+S D LYEYYNQ ++     MIN+  + +S   +  V K    TT
Sbjct: 1047 QNVEGLIHTIVNCIVKSGDELYEYYNQQIVEYHLKMINTHWYTISLPRNNPV-KNQKPTT 1106

BLAST of Spg021525 vs. NCBI nr
Match: KAA0039330.1 (TMV resistance protein N-like [Cucumis melo var. makuwa] >TYK00512.1 TMV resistance protein N-like [Cucumis melo var. makuwa])

HSP 1 Score: 650.2 bits (1676), Expect = 2.0e-182
Identity = 409/923 (44.31%), Postives = 507/923 (54.93%), Query Frame = 0

Query: 11   QNLQTLALMSTGLESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRL 70
            +NLQTL L  TGLE  P+I G+MEHLT LHL GS IT LHPSI  LTGL  L+LS C  L
Sbjct: 705  KNLQTLKLSGTGLEIFPEI-GHMEHLTHLHLDGSKITHLHPSIGYLTGLVFLDLSTCLGL 764

Query: 71   LSLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISGTSVSHVPTS-MHHLKN 130
             SLP EIGNL++LK L+L+ C +L +IPPSLANAESLE L+IS TS++HVP+S +H LKN
Sbjct: 765  SSLPFEIGNLKSLKTLLLKYCKRLDKIPPSLANAESLETLSISETSITHVPSSIIHCLKN 824

Query: 131  L----------------------------------------IRLVDEDVPKDLHCFSSLE 190
            L                                         +L+DED+P+DLHCFSSLE
Sbjct: 825  LETLDCEGLSRGIWKSLLPQLNINQTITTGLGCLKALNLMGCKLMDEDIPEDLHCFSSLE 884

Query: 191  ELNLSYNEFIKLPDSLSHLKKLKRLYLNYCRKLKDLPKLPTSLENVVAVGCISISEKFYN 250
             L+LSYN F  LPDSLSHLKKLK L LNYC +LKDLPKLP SL+ V  V C S+SE++YN
Sbjct: 885  ALDLSYNNFTTLPDSLSHLKKLKTLNLNYCTELKDLPKLPESLQYVGGVDCRSMSEQYYN 944

Query: 251  KRLHIFSDLKENLLLEFSISSKTTKVECNVNELQYTMLDRR------------------- 310
            K L I S     L L F I SK   VEC +NE Q+++  RR                   
Sbjct: 945  KILLIPSSSGHQLYLTFIIPSKDADVECAMNEFQHSIFTRRSFEQSIIEEQPSTIVHDTV 1004

Query: 311  ----------------------------------------LCIYF--------------- 370
                                                    +C  F               
Sbjct: 1005 DMFQWFGQINEGNWTNIQYEQEFSISKPLNIMYEDVDLSNVCGVFLSTNIEFPQNLNHLA 1064

Query: 371  ------------------------------------------------------------ 430
                                                                        
Sbjct: 1065 IGRFLVSFGIDGKCSGGTMNYEMSQFKAARFFWVAYIPIWMIKDHSLMVQRCCSVKVTIS 1124

Query: 431  -----------------------------------------------------------R 490
                                                                       R
Sbjct: 1125 YCCDHIDASKVKIKACGVSSMLSWPNVAEYLAKLFTERFCSKRNFYTMIRQHNDHQTECR 1184

Query: 491  CDELEIAKDDFPSSAFESTETESTFLPRKNLRSILERIFEEDKSCFQVSYFPQTKVSRWF 550
            CDELE+ KDDF SS FES   +STFL RKNLR+IL  +FEE K  +   +FP TK+  WF
Sbjct: 1185 CDELEVGKDDFSSSTFES--NDSTFLLRKNLRAILGVMFEEKKRYYMKYFFPHTKIFGWF 1244

Query: 551  RNQNKKDKVAIKLPLNIRKDKKWMGLAAFGIFSISQNANCYCLSYEVQLDDTCL--YRDH 610
            +NQNKKDKVA+K+P+NI KD+KWMGLA F +FSIS+ A+CYC  YE+Q  +  +   R  
Sbjct: 1245 KNQNKKDKVAVKIPVNIEKDRKWMGLAMFVVFSISEKASCYCFEYEIQTKENIISTQRHF 1304

Query: 611  IPM-KLLEYSNRILILVFKPS-DCYTYE--RNSSSNHVYISFNTNVERMQVESCGARLVY 670
            I   ++LE+SN+IL + F+P  + Y Y+  ++SSSNHVYI+FNTN  RM+VE CGARLVY
Sbjct: 1305 ISTDQVLEHSNQILFVAFEPRYNWYPYDELKSSSSNHVYINFNTNGARMRVEFCGARLVY 1364

Query: 671  KDNVKGLIQTIVDCIVESEDGLYEYYNQILMADLFSMINSSKHKVSSLESASVNKPATTT 691
            + NV+GLI TI++CI ES D LYE+YNQ ++    + IN+  + +S   + SV K   +T
Sbjct: 1365 QQNVEGLIHTILNCIAESGDELYEHYNQYIVESHLTFINTHWYTLSFRRNNSV-KNQPST 1424

BLAST of Spg021525 vs. NCBI nr
Match: XP_011656060.2 (TMV resistance protein N isoform X1 [Cucumis sativus] >XP_011656061.2 TMV resistance protein N isoform X1 [Cucumis sativus] >XP_011656062.2 TMV resistance protein N isoform X1 [Cucumis sativus] >XP_031741444.1 TMV resistance protein N isoform X1 [Cucumis sativus] >KAE8648981.1 hypothetical protein Csa_009304 [Cucumis sativus])

HSP 1 Score: 649.0 bits (1673), Expect = 4.5e-182
Identity = 404/923 (43.77%), Postives = 505/923 (54.71%), Query Frame = 0

Query: 11   QNLQTLALMSTGLESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRL 70
            +NLQTL L  TGLE  P+I G+MEHLT LHL GSNIT  HPSI  LTGL  L+LS C  L
Sbjct: 705  KNLQTLKLSGTGLEIFPEI-GHMEHLTHLHLDGSNITHFHPSIGYLTGLVFLDLSSCLGL 764

Query: 71   LSLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISGTSVSHVPTS-MHHLKN 130
             SLP EIGNL++LK L+L+ C KL +IPPSLANAESLE L+IS TS++HVP S +H LKN
Sbjct: 765  SSLPCEIGNLKSLKTLLLKYCKKLDKIPPSLANAESLETLSISETSITHVPPSIIHCLKN 824

Query: 131  L----------------------------------------IRLVDEDVPKDLHCFSSLE 190
            L                                         +L+DED+P+DLHCFSSLE
Sbjct: 825  LKTLDCEGLSHGIWKSLLPQFNINQTITTGLGCLKALNLMGCKLMDEDIPEDLHCFSSLE 884

Query: 191  ELNLSYNEFIKLPDSLSHLKKLKRLYLNYCRKLKDLPKLPTSLENVVAVGCISISEKFYN 250
             L+LSYN F  LPDSLSHLKKLK L LN C +LKDLPKLP SL+ V  + C S+SE++YN
Sbjct: 885  TLDLSYNNFTTLPDSLSHLKKLKTLNLNCCTELKDLPKLPESLQYVGGIDCRSMSERYYN 944

Query: 251  KRLHIFSDLKENLLLEFSISSKTTKVECNVNELQYTMLDRR------------------- 310
            K L I S     L L F I SK   VEC++NE Q+++  RR                   
Sbjct: 945  KILLIPSSSGHQLYLTFIIPSKDVDVECDMNEFQHSIFTRRSFELNIIEEKPSMIVHDAV 1004

Query: 311  ----------------------------------------LCIYF--------------- 370
                                                    +C  F               
Sbjct: 1005 DMFHWFGQINEGNWTNIQYEQEFSISKPLNIMYEDVDLSNVCGVFLSTNIEFPENLNHLA 1064

Query: 371  ------------------------------------------------------------ 430
                                                                        
Sbjct: 1065 IGRFLVSFEIDGKCSGGTMNYEMSQFKAARFFWAAYIPIWMFKDHSVMVQRCCSMKVTIS 1124

Query: 431  -----------------------------------------------------------R 490
                                                                       R
Sbjct: 1125 YCCDHIDASKVKIKACGVSSMLSWPNVAEYLAKLFTKRFCSKRNFYTMIRQHNDHQNECR 1184

Query: 491  CDELEIAKDDFPSSAFESTETESTFLPRKNLRSILERIFEEDKSCFQVSYFPQTKVSRWF 550
            CDELE+ KDDF SS FES   +STFL RKNLR+IL  +FEE K  +   +FP T +  WF
Sbjct: 1185 CDELEVRKDDFSSSTFES--NDSTFLLRKNLRAILGVMFEEKKRYYMKYFFPHTNIFGWF 1244

Query: 551  RNQNKKDKVAIKLPLNIRKDKKWMGLAAFGIFSISQNANCYCLSYEVQLDDTCLYRDHIP 610
            +NQNKKDKVA+K+P+NI KD+KWMGLA F +FSIS+ A+CYC  YE+Q  +  +      
Sbjct: 1245 KNQNKKDKVAVKIPVNIEKDRKWMGLAMFVVFSISEKASCYCFEYEIQTKEKIISTQRHS 1304

Query: 611  M---KLLEYSNRILILVFKPS-DCYTYE--RNSSSNHVYISFNTNVERMQVESCGARLVY 670
            +   ++LEYSN+IL + F+P  + Y Y+  ++SSSNHV+I+FNTN  RM+VE CGARLVY
Sbjct: 1305 ISTDQVLEYSNQILFVAFEPRYNWYPYDELKSSSSNHVFINFNTNGARMRVEFCGARLVY 1364

Query: 671  KDNVKGLIQTIVDCIVESEDGLYEYYNQILMADLFSMINSSKHKVSSLESASVNKPATTT 691
            + NV+GLI TI++CI ES D LYEYYNQ ++    ++IN+  + +S   + SV K   +T
Sbjct: 1365 QQNVEGLIHTIMNCIEESGDELYEYYNQQIVESHLNLINAHWYTISFRRNNSV-KNQPST 1424

BLAST of Spg021525 vs. NCBI nr
Match: XP_008459543.1 (PREDICTED: TMV resistance protein N-like [Cucumis melo] >XP_008459544.1 PREDICTED: TMV resistance protein N-like [Cucumis melo] >XP_008459545.1 PREDICTED: TMV resistance protein N-like [Cucumis melo] >XP_016902414.1 PREDICTED: TMV resistance protein N-like [Cucumis melo] >XP_016902415.1 PREDICTED: TMV resistance protein N-like [Cucumis melo] >XP_016902416.1 PREDICTED: TMV resistance protein N-like [Cucumis melo] >XP_016902417.1 PREDICTED: TMV resistance protein N-like [Cucumis melo])

HSP 1 Score: 647.1 bits (1668), Expect = 1.7e-181
Identity = 405/923 (43.88%), Postives = 505/923 (54.71%), Query Frame = 0

Query: 11   QNLQTLALMSTGLESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRL 70
            +NLQTL L  TGLE  P+I G+MEHLT LHL GS IT LHPSI  LTGL  L+LS C  L
Sbjct: 705  KNLQTLKLSGTGLEIFPEI-GHMEHLTHLHLDGSKITHLHPSIGYLTGLVFLDLSTCLGL 764

Query: 71   LSLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISGTSVSHVPTS-MHHLKN 130
             SLP EIGNL++LK L+L+ C +L +IPPSLANAESLE L+IS TS++HVP+S +H LKN
Sbjct: 765  SSLPFEIGNLKSLKTLLLKYCKRLDKIPPSLANAESLETLSISETSITHVPSSIIHCLKN 824

Query: 131  L----------------------------------------IRLVDEDVPKDLHCFSSLE 190
            L                                         +L+DED+P+DLHCFSSLE
Sbjct: 825  LETLDCEELSRGIWKSLLPQLNINQTITTGLGCLKALNLMGCKLMDEDIPEDLHCFSSLE 884

Query: 191  ELNLSYNEFIKLPDSLSHLKKLKRLYLNYCRKLKDLPKLPTSLENVVAVGCISISEKFYN 250
             L+LSYN F  LPDSLSHLKKLK L LNYC +LKDLPKLP SL+ V  V C S+SE++YN
Sbjct: 885  TLDLSYNNFTTLPDSLSHLKKLKTLILNYCTELKDLPKLPESLQYVGGVDCRSMSEQYYN 944

Query: 251  KRLHIFSDLKENLLLEFSISSKTTKVECNVNELQYTMLDRR------------------- 310
            K L I S     L L F I SK   VEC +NE Q+++  RR                   
Sbjct: 945  KILLIPSSSGHQLYLTFIIPSKDADVECVMNEFQHSIFTRRSFEQSIIEKKPSPIFHDTV 1004

Query: 311  ----------------------------------------LCIYF--------------- 370
                                                    +C  F               
Sbjct: 1005 DMFQWFGQINEGNWTNIQYEQEFSISKPLNIMYEDVNLSNVCGVFLSTNIEFPQNLNHLA 1064

Query: 371  ------------------------------------------------------------ 430
                                                                        
Sbjct: 1065 IGRFLVSFEIDGKCSGGTMNYKMSQFKAARFFWVAYIPIWMNKDHSLMVQRCCSVKVTIS 1124

Query: 431  -----------------------------------------------------------R 490
                                                                       R
Sbjct: 1125 YCCDHIDASKVKIKACGVSSMLSWPNVAEYLAKLFTERFCSKRNFYTMIRQHNDHQTECR 1184

Query: 491  CDELEIAKDDFPSSAFESTETESTFLPRKNLRSILERIFEEDKSCFQVSYFPQTKVSRWF 550
            CDELE+ KDDF SS FES   +STFL RKNLR+IL  +FE  K  +   +FP TK+  WF
Sbjct: 1185 CDELEVGKDDFSSSTFES--NDSTFLLRKNLRAILGVMFEGKKRYYMKYFFPHTKIFGWF 1244

Query: 551  RNQNKKDKVAIKLPLNIRKDKKWMGLAAFGIFSISQNANCYCLSYEVQLDDTCLYRDHIP 610
            +NQNKKDKVA+K+P+NI KD+KWMGLA F +FSIS+ A+CYC  YE+Q  +  +      
Sbjct: 1245 KNQNKKDKVAVKIPVNIEKDRKWMGLAMFVVFSISEKASCYCFEYEIQTKENIISTQSHS 1304

Query: 611  M---KLLEYSNRILILVFKPS-DCYTYE--RNSSSNHVYISFNTNVERMQVESCGARLVY 670
            +   ++LE+SN+IL + F+P  + Y Y+  ++SSSNHVYI+FNTN  RM+VE CGARLVY
Sbjct: 1305 ISTDQVLEHSNQILFVAFEPRYNWYPYDELKSSSSNHVYINFNTNGARMRVEFCGARLVY 1364

Query: 671  KDNVKGLIQTIVDCIVESEDGLYEYYNQILMADLFSMINSSKHKVSSLESASVNKPATTT 691
            + NV+GL+ TI++CI ES D LYE+YNQ ++    + IN+  + +S   + SV K   +T
Sbjct: 1365 QQNVEGLVHTILNCIAESGDELYEHYNQYIVESHLTFINTHWYTLSFRRNNSV-KNQPST 1424

BLAST of Spg021525 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.4e-16
Identity = 57/172 (33.14%), Postives = 98/172 (56.98%), Query Frame = 0

Query: 32  NMEHLTQLHLAGS-NITQLHPSIECLTGLALLNLSHCTRLLSLPTEIGNLRALKILILQC 91
           +M +L +L L G  ++  +HPS+  +  L  L+L  C +L +LP  IG+L +L+IL L  
Sbjct: 683 SMPNLERLFLRGCVSLIDIHPSVGNMKKLTTLSLKSCKKLKNLPDSIGDLESLEILDLAY 742

Query: 92  CTKLYQIPPSLANAESLEKLNISGTSVSHVPTSMHHLKNL--IRLVD----EDVPKDLHC 151
           C+K  + P    N +SL +L++  T++  +P S+  L++L  + L D    E  P+    
Sbjct: 743 CSKFEKFPEKGGNMKSLTELDLQNTAIKDLPDSIGDLESLKYLDLSDCSKFEKFPEKGGN 802

Query: 152 FSSLEELNLSYNEFIKLPDSLSHLKKLKRLYLNYCRKLKDLPKLPTSLENVV 197
             SL EL+L       LPDS+  L+ L+RLYL+YC K +  P+   ++++++
Sbjct: 803 MKSLRELDLRNTAIKDLPDSIRDLESLERLYLSYCSKFEKFPEKGGNMKSLM 854

BLAST of Spg021525 vs. ExPASy Swiss-Prot
Match: F4JT82 (Probable disease resistance protein At4g19520 OS=Arabidopsis thaliana OX=3702 GN=At4g19520 PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.3e-16
Identity = 62/175 (35.43%), Postives = 92/175 (52.57%), Query Frame = 0

Query: 35   HLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRLLSLPTEIGNLRALKILILQCCTKL 94
            +L +L+LAG+ I +L PSI  L  L  L+L +C RL  LP E+ NL  LK+L L  C++L
Sbjct: 894  NLIELYLAGTAIRELPPSIGDLALLDTLDLKNCNRLRHLPMEMHNLNPLKVLDLSNCSEL 953

Query: 95   YQIPPSLANAESLEKLNISGTSVSHVPTSM-----HHLK-NLIRLVDEDVPKDLHCFSSL 154
                 SL     L          S +P        H +  +L +   + +P+++    SL
Sbjct: 954  EVFTSSLPKVRELRPAPTVMLLRSKLPFCFFIFYEHRVTLSLYKARLQYIPEEIRWMPSL 1013

Query: 155  EELNLSYNEFIKLPDSLSHLKKLKRLYLNYCRKLKDLPKLPTSLENVVAVGCISI 204
            + L+LS N F ++P S+    KL  L L YC  L+ LP+LP SL+ + A GC S+
Sbjct: 1014 KTLDLSRNGFTEVPVSIKDFSKLLSLRLRYCENLRSLPQLPRSLQLLNAHGCSSL 1068

BLAST of Spg021525 vs. ExPASy Swiss-Prot
Match: Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 4.0e-16
Identity = 76/227 (33.48%), Postives = 109/227 (48.02%), Query Frame = 0

Query: 11  QNLQTLALMS-TGLESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTR 70
           Q+LQTL L   + L+  P I  N+E L    L G+ I  L  SI+    LALLNL +C +
Sbjct: 713 QSLQTLILSGCSSLKKFPLISENVEVLL---LDGTVIKSLPESIQTFRRLALLNLKNCKK 772

Query: 71  LLSLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISGTSVSHVPTSMHHLKN 130
           L  L +++  L+ L+ LIL  C++L   P    + ESLE L +  TS++ +P  M HL N
Sbjct: 773 LKHLSSDLYKLKCLQELILSGCSQLEVFPEIKEDMESLEILLMDDTSITEMP-KMMHLSN 832

Query: 131 L-------------------------IRLVD--------EDVPKDLHCFSSLEELNLSYN 190
           +                          RL D          +P ++   SSL+ L LS N
Sbjct: 833 IKTFSLCGTSSHVSVSMFFMPPTLGCSRLTDLYLSRCSLYKLPDNIGGLSSLQSLCLSGN 892

Query: 191 EFIKLPDSLSHLKKLKRLYLNYCRKLKDLPKLPTSLENVVAVGCISI 204
               LP+S + L  LK   L +C+ LK LP LP +L+ + A  C S+
Sbjct: 893 NIENLPESFNQLNNLKWFDLKFCKMLKSLPVLPQNLQYLDAHECESL 935

BLAST of Spg021525 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 3.4e-15
Identity = 94/371 (25.34%), Postives = 158/371 (42.59%), Query Frame = 0

Query: 11   QNLQTLALMS-TGLESSPKIGGNMEHLTQLHLAGSNITQLHPSI-ECLTGLALLNLSHCT 70
            ++L+ L L S   LE  P+I G M+   Q+H+ GS I +L  SI +  T +  L L +  
Sbjct: 688  ESLEYLGLRSCDSLEKLPEIYGRMKPEIQIHMQGSGIRELPSSIFQYKTHVTKLLLWNMK 747

Query: 71   RLLSLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISGTSVSHVPTSMHHLK 130
             L++LP+ I  L++L  L +  C+KL  +P  + + ++L   + S T +   P+S+  L 
Sbjct: 748  NLVALPSSICRLKSLVSLSVSGCSKLESLPEEIGDLDNLRVFDASDTLILRPPSSIIRLN 807

Query: 131  NLI---------------------------------RLVDEDVPKDLHCFSSLEELNLSY 190
             LI                                  L+D  +P+++   SSL++L+LS 
Sbjct: 808  KLIILMFRGFKDGVHFEFPPVAEGLHSLEYLNLSYCNLIDGGLPEEIGSLSSLKKLDLSR 867

Query: 191  NEFIKLPDSLSHLKKLKRLYLNYCRKLKDLPKLPTSLENVVAVGCISISEKFYNKRLHIF 250
            N F  LP S++ L  L+ L L  C++L  LP+LP  L N + V C               
Sbjct: 868  NNFEHLPSSIAQLGALQSLDLKDCQRLTQLPELPPEL-NELHVDC--------------- 927

Query: 251  SDLKENLLLEFSISSKTTKVECNVNELQYTMLDRRLCIYFRCDELEIAKDDFPSSAFEST 310
                 ++ L+F               + Y +  R+     + D+   A +D   + F  T
Sbjct: 928  -----HMALKF---------------IHYLVTKRKKLHRVKLDD---AHNDTMYNLFAYT 987

Query: 311  ETESTFLPRKNLRSILERIFEEDK---SCFQVSYFPQTKVSRWFRNQNKKDKVAIKLPLN 344
              +       N+ S+   I   D    + F    +P+ K+  WF +Q     V++ LP N
Sbjct: 988  MFQ-------NISSMRHDISASDSLSLTVFTGQPYPE-KIPSWFHHQGWDSSVSVNLPEN 1011

BLAST of Spg021525 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 5.8e-15
Identity = 71/240 (29.58%), Postives = 110/240 (45.83%), Query Frame = 0

Query: 24   ESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHC---------------- 83
            E  P+ GGNM+ L +L L  + I  L  SI  L  L  L+LS C                
Sbjct: 835  EKFPEKGGNMKSLKELDLQNTAIKDLPDSIGDLKSLKYLSLSDCSKFEKFPEKGGNMKRL 894

Query: 84   -------TRLLSLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISGTSVSHV 143
                   T +  LP  IG+L +LK L L  C+K  + P    N +SL +L++  T++  +
Sbjct: 895  LQLILSNTAIKDLPDSIGDLESLKYLYLSDCSKFEKFPEKGGNMKSLTELDLKNTAIKDL 954

Query: 144  PTSMHHLKNL--IRLVD----EDVPKDLHCFSSLEELNLSYNEFIKLPDSLSHLKKLKRL 203
            P S+  L++L  + L D    E  P+      SL+EL+L       LPDS+  L+ LK L
Sbjct: 955  PDSIGDLESLEILNLSDCAKFEKFPEKGGNMKSLKELDLQNTAIKDLPDSIGDLESLKYL 1014

Query: 204  YLNYCRKLKDLPKLPTSLENVVAVGCISISEKFYNKRLHIFSDLKENLLLEFSISSKTTK 235
            YL+ C K +  P+   ++++++    + +S            DL+    L  S+ SK  K
Sbjct: 1015 YLSDCSKFEKFPEKGGNMKSLLQ---LILSNTAIKDLPDSIGDLESLEYLHLSVCSKFEK 1071

BLAST of Spg021525 vs. ExPASy TrEMBL
Match: A0A5A7T7V5 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001220 PE=4 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 9.7e-183
Identity = 409/923 (44.31%), Postives = 507/923 (54.93%), Query Frame = 0

Query: 11   QNLQTLALMSTGLESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRL 70
            +NLQTL L  TGLE  P+I G+MEHLT LHL GS IT LHPSI  LTGL  L+LS C  L
Sbjct: 705  KNLQTLKLSGTGLEIFPEI-GHMEHLTHLHLDGSKITHLHPSIGYLTGLVFLDLSTCLGL 764

Query: 71   LSLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISGTSVSHVPTS-MHHLKN 130
             SLP EIGNL++LK L+L+ C +L +IPPSLANAESLE L+IS TS++HVP+S +H LKN
Sbjct: 765  SSLPFEIGNLKSLKTLLLKYCKRLDKIPPSLANAESLETLSISETSITHVPSSIIHCLKN 824

Query: 131  L----------------------------------------IRLVDEDVPKDLHCFSSLE 190
            L                                         +L+DED+P+DLHCFSSLE
Sbjct: 825  LETLDCEGLSRGIWKSLLPQLNINQTITTGLGCLKALNLMGCKLMDEDIPEDLHCFSSLE 884

Query: 191  ELNLSYNEFIKLPDSLSHLKKLKRLYLNYCRKLKDLPKLPTSLENVVAVGCISISEKFYN 250
             L+LSYN F  LPDSLSHLKKLK L LNYC +LKDLPKLP SL+ V  V C S+SE++YN
Sbjct: 885  ALDLSYNNFTTLPDSLSHLKKLKTLNLNYCTELKDLPKLPESLQYVGGVDCRSMSEQYYN 944

Query: 251  KRLHIFSDLKENLLLEFSISSKTTKVECNVNELQYTMLDRR------------------- 310
            K L I S     L L F I SK   VEC +NE Q+++  RR                   
Sbjct: 945  KILLIPSSSGHQLYLTFIIPSKDADVECAMNEFQHSIFTRRSFEQSIIEEQPSTIVHDTV 1004

Query: 311  ----------------------------------------LCIYF--------------- 370
                                                    +C  F               
Sbjct: 1005 DMFQWFGQINEGNWTNIQYEQEFSISKPLNIMYEDVDLSNVCGVFLSTNIEFPQNLNHLA 1064

Query: 371  ------------------------------------------------------------ 430
                                                                        
Sbjct: 1065 IGRFLVSFGIDGKCSGGTMNYEMSQFKAARFFWVAYIPIWMIKDHSLMVQRCCSVKVTIS 1124

Query: 431  -----------------------------------------------------------R 490
                                                                       R
Sbjct: 1125 YCCDHIDASKVKIKACGVSSMLSWPNVAEYLAKLFTERFCSKRNFYTMIRQHNDHQTECR 1184

Query: 491  CDELEIAKDDFPSSAFESTETESTFLPRKNLRSILERIFEEDKSCFQVSYFPQTKVSRWF 550
            CDELE+ KDDF SS FES   +STFL RKNLR+IL  +FEE K  +   +FP TK+  WF
Sbjct: 1185 CDELEVGKDDFSSSTFES--NDSTFLLRKNLRAILGVMFEEKKRYYMKYFFPHTKIFGWF 1244

Query: 551  RNQNKKDKVAIKLPLNIRKDKKWMGLAAFGIFSISQNANCYCLSYEVQLDDTCL--YRDH 610
            +NQNKKDKVA+K+P+NI KD+KWMGLA F +FSIS+ A+CYC  YE+Q  +  +   R  
Sbjct: 1245 KNQNKKDKVAVKIPVNIEKDRKWMGLAMFVVFSISEKASCYCFEYEIQTKENIISTQRHF 1304

Query: 611  IPM-KLLEYSNRILILVFKPS-DCYTYE--RNSSSNHVYISFNTNVERMQVESCGARLVY 670
            I   ++LE+SN+IL + F+P  + Y Y+  ++SSSNHVYI+FNTN  RM+VE CGARLVY
Sbjct: 1305 ISTDQVLEHSNQILFVAFEPRYNWYPYDELKSSSSNHVYINFNTNGARMRVEFCGARLVY 1364

Query: 671  KDNVKGLIQTIVDCIVESEDGLYEYYNQILMADLFSMINSSKHKVSSLESASVNKPATTT 691
            + NV+GLI TI++CI ES D LYE+YNQ ++    + IN+  + +S   + SV K   +T
Sbjct: 1365 QQNVEGLIHTILNCIAESGDELYEHYNQYIVESHLTFINTHWYTLSFRRNNSV-KNQPST 1424

BLAST of Spg021525 vs. ExPASy TrEMBL
Match: A0A1S4E362 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498648 PE=4 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 8.2e-182
Identity = 405/923 (43.88%), Postives = 505/923 (54.71%), Query Frame = 0

Query: 11   QNLQTLALMSTGLESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRL 70
            +NLQTL L  TGLE  P+I G+MEHLT LHL GS IT LHPSI  LTGL  L+LS C  L
Sbjct: 705  KNLQTLKLSGTGLEIFPEI-GHMEHLTHLHLDGSKITHLHPSIGYLTGLVFLDLSTCLGL 764

Query: 71   LSLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISGTSVSHVPTS-MHHLKN 130
             SLP EIGNL++LK L+L+ C +L +IPPSLANAESLE L+IS TS++HVP+S +H LKN
Sbjct: 765  SSLPFEIGNLKSLKTLLLKYCKRLDKIPPSLANAESLETLSISETSITHVPSSIIHCLKN 824

Query: 131  L----------------------------------------IRLVDEDVPKDLHCFSSLE 190
            L                                         +L+DED+P+DLHCFSSLE
Sbjct: 825  LETLDCEELSRGIWKSLLPQLNINQTITTGLGCLKALNLMGCKLMDEDIPEDLHCFSSLE 884

Query: 191  ELNLSYNEFIKLPDSLSHLKKLKRLYLNYCRKLKDLPKLPTSLENVVAVGCISISEKFYN 250
             L+LSYN F  LPDSLSHLKKLK L LNYC +LKDLPKLP SL+ V  V C S+SE++YN
Sbjct: 885  TLDLSYNNFTTLPDSLSHLKKLKTLILNYCTELKDLPKLPESLQYVGGVDCRSMSEQYYN 944

Query: 251  KRLHIFSDLKENLLLEFSISSKTTKVECNVNELQYTMLDRR------------------- 310
            K L I S     L L F I SK   VEC +NE Q+++  RR                   
Sbjct: 945  KILLIPSSSGHQLYLTFIIPSKDADVECVMNEFQHSIFTRRSFEQSIIEKKPSPIFHDTV 1004

Query: 311  ----------------------------------------LCIYF--------------- 370
                                                    +C  F               
Sbjct: 1005 DMFQWFGQINEGNWTNIQYEQEFSISKPLNIMYEDVNLSNVCGVFLSTNIEFPQNLNHLA 1064

Query: 371  ------------------------------------------------------------ 430
                                                                        
Sbjct: 1065 IGRFLVSFEIDGKCSGGTMNYKMSQFKAARFFWVAYIPIWMNKDHSLMVQRCCSVKVTIS 1124

Query: 431  -----------------------------------------------------------R 490
                                                                       R
Sbjct: 1125 YCCDHIDASKVKIKACGVSSMLSWPNVAEYLAKLFTERFCSKRNFYTMIRQHNDHQTECR 1184

Query: 491  CDELEIAKDDFPSSAFESTETESTFLPRKNLRSILERIFEEDKSCFQVSYFPQTKVSRWF 550
            CDELE+ KDDF SS FES   +STFL RKNLR+IL  +FE  K  +   +FP TK+  WF
Sbjct: 1185 CDELEVGKDDFSSSTFES--NDSTFLLRKNLRAILGVMFEGKKRYYMKYFFPHTKIFGWF 1244

Query: 551  RNQNKKDKVAIKLPLNIRKDKKWMGLAAFGIFSISQNANCYCLSYEVQLDDTCLYRDHIP 610
            +NQNKKDKVA+K+P+NI KD+KWMGLA F +FSIS+ A+CYC  YE+Q  +  +      
Sbjct: 1245 KNQNKKDKVAVKIPVNIEKDRKWMGLAMFVVFSISEKASCYCFEYEIQTKENIISTQSHS 1304

Query: 611  M---KLLEYSNRILILVFKPS-DCYTYE--RNSSSNHVYISFNTNVERMQVESCGARLVY 670
            +   ++LE+SN+IL + F+P  + Y Y+  ++SSSNHVYI+FNTN  RM+VE CGARLVY
Sbjct: 1305 ISTDQVLEHSNQILFVAFEPRYNWYPYDELKSSSSNHVYINFNTNGARMRVEFCGARLVY 1364

Query: 671  KDNVKGLIQTIVDCIVESEDGLYEYYNQILMADLFSMINSSKHKVSSLESASVNKPATTT 691
            + NV+GL+ TI++CI ES D LYE+YNQ ++    + IN+  + +S   + SV K   +T
Sbjct: 1365 QQNVEGLVHTILNCIAESGDELYEHYNQYIVESHLTFINTHWYTLSFRRNNSV-KNQPST 1424

BLAST of Spg021525 vs. ExPASy TrEMBL
Match: A0A6J1CL15 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012122 PE=4 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 3.7e-105
Identity = 277/895 (30.95%), Postives = 426/895 (47.60%), Query Frame = 0

Query: 11   QNLQTLALMSTGLESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRL 70
            ++L+ L L  +GL SSPK   NMEHL++LHL G+ I QLH SI CL GL LL+L +C  L
Sbjct: 745  KSLEGLILSESGLTSSPKFEVNMEHLSRLHLVGTPIKQLHSSIGCLIGLVLLDLRNCIML 804

Query: 71   LSLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISGTSVSHVPTSMHHLKNL 130
             SLP+EIGNL++L++L+L+ C  L QIPPSL NA+ LE L+I  TS+   P ++H+L+NL
Sbjct: 805  SSLPSEIGNLKSLQVLLLEGCKSLDQIPPSLGNAQCLEYLDIGKTSICQAPPTIHYLENL 864

Query: 131  IR---------------------------------------------LVDEDVPKDLHCF 190
             R                                             LVDED+P+DLHCF
Sbjct: 865  KRLNCEGLSRKIWHSLLPRDIQRSNRQISGLGFGLKSLEHLCLKNCNLVDEDIPEDLHCF 924

Query: 191  SSLEELNLSYNEFIKLPDSLSHLKKLKRLYLNYCRKLKDLPK------------------ 250
            SSLE L+LS N F++ P S++HL  LK+L  + C KL+ +PK                  
Sbjct: 925  SSLESLDLSGNHFVQYPKSINHLVNLKQLMFHDCFKLQQIPKPSINLEPKFSSVESGMPI 984

Query: 251  ---LPTSLE--------------------------------------------------- 310
               LP  L+                                                   
Sbjct: 985  DQSLPCQLDKPFRLEYGSEIKSKLDPKTYEELILERATFGTTCDKAEILECFDEIREGNS 1044

Query: 311  ------------NVVAVGCISIS------------EKFY-NKRLHIFSDLKENLLLEFSI 370
                        N++    +S S            + FY N  + + +D+   + + +  
Sbjct: 1045 VEVEYNQVKNDANIIYGVVLSASIQCSKNTSHNEVDTFYCNFSIELETDMNPTITVFYGT 1104

Query: 371  SSKTTKVE---------------------------------------C--------NVNE 430
             + T+++                                        C        N ++
Sbjct: 1105 FALTSRIRFWTLFIPSHKLPKSTSMQNSLCSFKATIKYEVSSVHIQMCGASILSHQNASQ 1164

Query: 431  LQYTMLDRRLCIYFR---------------CDELEIAKDDFPSSAFESTETESTFLPRKN 490
                M  R  CI  +               CDE E  + ++    F   E +S  L R+N
Sbjct: 1165 FMAKMFSRMYCIQEKFQTFIVGGNHWSECNCDEPETVEHNY-LIQFVENERDSFSLLRRN 1224

Query: 491  LRSILERIFEEDKSCFQVSYFPQTKVSRWFRNQNKKDKVAIKLPLNIRKDKKWMGLAAFG 550
            L+SIL R +E+ K  F   +FPQ +V  WFR Q   D V +KLP N+ ++KKWMGLA F 
Sbjct: 1225 LKSILRRTYEDTKHIFPFYFFPQNQVPSWFRIQQLNDTVKMKLPPNMFREKKWMGLAMFA 1284

Query: 551  IFSISQNANC---YCLSYEVQLDDTCLYRDH-----------IPMKLLEYSNRILILVFK 610
            +FS+ +N+     +  S+++ +D+   Y              IP++LL  S++IL ++FK
Sbjct: 1285 VFSVERNSEFVKDHPFSWQIDIDENGTYLKRVRSYAFHSPHMIPLQLLWSSSKILFMIFK 1344

Query: 611  PSDCYTYERNSSSNHVYISFNTNVERMQVESCGARLVYKDNVKGLIQTIVDCIVESEDGL 670
            P +   Y ++  SNH+Y+ F TN   ++VE CGARLVY+ NV G I  IVDC+++SE   
Sbjct: 1345 PRETSPYVQD-KSNHLYVKFLTNSTSLKVELCGARLVYQHNVDGFIHKIVDCVLKSEQKQ 1404

Query: 671  YEYYNQILMADLFSMINSSKHKVSSLESASVNKPATTTTALTDTDSSLLVEQLLYGSFPH 686
             + Y+  ++ DL          +++ +     K AT+T+   +    L  E L + S   
Sbjct: 1405 CQNYSHKILRDL----------LATYQQPIWKKVATSTS--NNPTQHLSTETLRHESSQR 1464

BLAST of Spg021525 vs. ExPASy TrEMBL
Match: A0A6J1CJ31 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012055 PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 4.2e-101
Identity = 279/889 (31.38%), Postives = 419/889 (47.13%), Query Frame = 0

Query: 11   QNLQTLALMSTGLESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRL 70
            +NL+ L L  +G+ SSP+I  NME L +L+L G+ I QLHPSI  L GL  L+L  C  L
Sbjct: 730  KNLERLVLSKSGVTSSPEIEENMERLCELYLDGTPIKQLHPSIGRLVGLIKLDLRKCIML 789

Query: 71   LSLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISGTSVSHVPTSMHHLKNL 130
             SLP EIGNL +L+ L+L  C  L Q+PPSL N + L+ L I  TS+ H+P ++H LKNL
Sbjct: 790  SSLPNEIGNLESLQSLLLGGCINLDQMPPSLGNVQRLQYLEICETSICHLPPTIHSLKNL 849

Query: 131  ----------------------------------------------IRLVDEDVPKDLHC 190
                                                            LV+ED+P+DLH 
Sbjct: 850  KGLKFDGLSGRIWHSLLPPSNILESDLGISGSKFGMESLEYLSLRGCNLVEEDIPEDLHY 909

Query: 191  FSSLEELNLSYNEFIKLPDSLSHLKKLKRLYLNYCRKLKDLPKLPTSL------------ 250
            FSSL+ L+LS N F++LP+S++HLK L+ L L+ C KL+ LP+L T+L            
Sbjct: 910  FSSLKTLDLSGNNFVRLPESINHLKNLRELRLHDCFKLQHLPELRTNLVPIISCTESGAV 969

Query: 251  ------------------------------ENVVAVGCISISEKFYNKRLHIFSDLKE-- 310
                                          E  ++ G +  S     + L  F +++E  
Sbjct: 970  RNLPIPYHHDKHFILEFIPKTNLKLDPKAFEESISEGALFSSTDDNAEVLECFDEIREGN 1029

Query: 311  ------------------------------------------------------------ 370
                                                                        
Sbjct: 1030 VIEIERYQIKNDANNIYGVVLSACIQCSQNTAHEVISFYCKFLIELGMEEHPRTGILPLT 1089

Query: 371  ----------------NLL----LEFSISSKTTKVECNV--------------------- 430
                            NLL    L F I +K +    N+                     
Sbjct: 1090 SKLTSRTRFWMLFIPLNLLPKSRLRFKILAKGSPNTINIQKCGASVLSHQNAPELLANMF 1149

Query: 431  NELQYTMLDRRLCIYFR---------CDELEIAKDDFPSSAFESTETESTFLPRKNLRSI 490
            N++  T      C   R         CDE E   D + S+ +   E+    L +++L+SI
Sbjct: 1150 NKVYRTRNQDEFCTTIRECNHGSKCSCDEPETI-DQYCSTQYVENESGPFSLLKRSLKSI 1209

Query: 491  LERIFEEDKSCFQVSYFPQTKVSRWFRNQNKKDKVAIKLPLNIRKDKKWMGLAAFGIFSI 550
            L R +EE+K      +FP+     WFR Q  KD VA+KLPL++ K+KKWMGLA F IFS+
Sbjct: 1210 LRRTYEEEKDHSLYYFFPRGPALSWFRIQQPKDTVAVKLPLDLFKEKKWMGLAVFAIFSV 1269

Query: 551  ---SQNANCYCLSYEVQLDDTCLYRDH----------IPMKLLEYSNRILILVFKPSDCY 610
               S+  + +  ++++  ++   +             IP+ LL+ S +IL + F+P   +
Sbjct: 1270 PGNSEYVHNHTFNFQITSNEDGRHLIKGGSYSSGSFLIPLSLLQSSPQILFVTFEPRHVF 1329

Query: 611  TYERNSSSNHVYISFNTNVERMQVESCGARLVYKDNVKGLIQTIVDCIVESEDGLYEYYN 670
             Y +    NH Y+SF +   R+QVESCGARLVY+ NV+GLI TI+DC+ +S + LY+ ++
Sbjct: 1330 PYVQ-EGLNHFYVSFISIPTRLQVESCGARLVYQHNVEGLINTIMDCVPKSREELYQDHS 1389

Query: 671  QILMADLFSMINSSKHKVSSLESASVNKPATTTTALTDTDSSLLVEQLLYGSFPHPLLQK 684
            Q +M D  +  +             +  PA   T  T +D S   E     SF H L  +
Sbjct: 1390 QEIMRDFLTTYH---------RPIRIQVPADQLTTSTSSDLSGRRE-----SFQHSLFME 1449

BLAST of Spg021525 vs. ExPASy TrEMBL
Match: A0A0A0KXU0 (TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G647530 PE=4 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 3.8e-86
Identity = 229/618 (37.06%), Postives = 282/618 (45.63%), Query Frame = 0

Query: 11   QNLQTLALMSTGLESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRL 70
            +NLQTL L  TGLE  P+I G+MEHLT LHL GSNIT  HPSI  LTGL  L+LS C  L
Sbjct: 772  KNLQTLKLSGTGLEIFPEI-GHMEHLTHLHLDGSNITHFHPSIGYLTGLVFLDLSSCLGL 831

Query: 71   LSLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISGTSVSHVPTS-MHHLKN 130
             SLP EIGNL++LK L+L+ C KL +IPPSLANAESLE L+IS TS++HVP S +H LKN
Sbjct: 832  SSLPCEIGNLKSLKTLLLKYCKKLDKIPPSLANAESLETLSISETSITHVPPSIIHCLKN 891

Query: 131  L----------------------------------------IRLVDEDVPKDLHCFSSLE 190
            L                                         +L+DED+P+DLHCFSSLE
Sbjct: 892  LKTLDCEGLSHGIWKSLLPQFNINQTITTGLGCLKALNLMGCKLMDEDIPEDLHCFSSLE 951

Query: 191  ELNLSYNEFIKLPDSLSHLKKLKRLYLNYCRKLKDLPKLPTSLENVVAVGCISISEKFYN 250
             L+LSYN F  LPDSLSHLKKLK L LN C +LKDLPKLP SL+ V  + C S+SE++YN
Sbjct: 952  TLDLSYNNFTTLPDSLSHLKKLKTLNLNCCTELKDLPKLPESLQYVGGIDCRSMSERYYN 1011

Query: 251  KRLHIFSDLKENLLLEFSISSKTTKVECNVNELQYTMLDRR------------------- 310
            K L I S     L L F I SK   VEC++NE Q+++  RR                   
Sbjct: 1012 KILLIPSSSGHQLYLTFIIPSKDVDVECDMNEFQHSIFTRRSFELNIIEEKPSMIVHDAV 1071

Query: 311  ----------------------------------------LCIYF--------------- 370
                                                    +C  F               
Sbjct: 1072 DMFHWFGQINEGNWTNIQYEQEFSISKPLNIMYEDVDLSNVCGVFLSTNIEFPENLNHLA 1131

Query: 371  ------------------------------------------------------------ 392
                                                                        
Sbjct: 1132 IGRFLVSFEIDGKCSGGTMNYEMSQFKAARFFWAAYIPIWMFKDHSVMVQRCCSMKVTIS 1191

BLAST of Spg021525 vs. TAIR 10
Match: AT1G69550.1 (disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 105.5 bits (262), Expect = 1.7e-22
Identity = 81/202 (40.10%), Postives = 120/202 (59.41%), Query Frame = 0

Query: 12   NLQTLALMS-TGLESSPKIGGNMEHLTQLHLAG-SNITQLHPSIECLTGLALLNLSHCTR 71
            NLQ L L   + L   P   GN+ +L +L L+G S++ +L  SI  L  L  LNLS C+ 
Sbjct: 1029 NLQELYLSECSSLVELPSSIGNLINLKKLDLSGCSSLVELPLSIGNLINLKTLNLSGCSS 1088

Query: 72   LLSLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISG-TSVSHVPTSMHHLK 131
            L+ LP+ IGNL  LK L L  C+ L ++P S+ N  +L+KL++SG +S+  +P S+ +L 
Sbjct: 1089 LVELPSSIGNLN-LKKLDLSGCSSLVELPSSIGNLINLKKLDLSGCSSLVELPLSIGNLI 1148

Query: 132  NLIRL-VDE-----DVPKDLHCFSSLEELNLS-YNEFIKLPDSLSHLKKLKRLYLNYCRK 191
            NL  L + E     ++P  +    +L+EL LS  +  ++LP S+ +L  LK+L LN C K
Sbjct: 1149 NLQELYLSECSSLVELPSSIGNLINLQELYLSECSSLVELPSSIGNLINLKKLDLNKCTK 1208

Query: 192  LKDLPKLPTSLENVVAVGCISI 204
            L  LP+LP SL  +VA  C S+
Sbjct: 1209 LVSLPQLPDSLSVLVAESCESL 1229

BLAST of Spg021525 vs. TAIR 10
Match: AT5G45200.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 102.8 bits (255), Expect = 1.1e-21
Identity = 125/504 (24.80%), Postives = 211/504 (41.87%), Query Frame = 0

Query: 34   EHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRLLSLPTEIGNLRALKILILQCCTK 93
            ++L +L+L G+ I +L  +I  L  L  L L  C  LLSLP  IGNL+A++ +IL  C+ 
Sbjct: 752  KNLEELYLDGTAIKELPSTIGDLQKLISLKLKDCKNLLSLPDSIGNLKAIQEIILSGCSS 811

Query: 94   LYQIPPSLANAESLEKLNISGTSVSHVPTSMHHLKNLIRLVDE-------DVPKDLHCFS 153
            L   P    N + L+ L + GT++  +P  +HHL     L          + P+ ++  S
Sbjct: 812  LESFPEVNQNLKHLKTLLLDGTAIKKIPDILHHLSPDQGLTSSQSNCHLCEWPRGIYGLS 871

Query: 154  SLEELNLSYNEFIKLPDSLSHLKKLKRLYLNYCRKLKDLPKLPTSLENVVAVGCISISEK 213
            S+  L+LS NEF  LP S+ +L  L  L L +C+ L  +P LP +L+ + A GCIS    
Sbjct: 872  SVRRLSLSSNEFRILPRSIGYLYHLNWLDLKHCKNLVSVPMLPPNLQWLDAHGCIS---- 931

Query: 214  FYNKRLHIFSDLKENLLLEFSISSKTTKVECNVNELQYTMLDRRLCIYFRCDELEIAKDD 273
                 L   S L + LL E                L  T       I+  C +L      
Sbjct: 932  -----LETISILSDPLLAE-------------TEHLHSTF------IFTNCTKL------ 991

Query: 274  FPSSAFESTETESTFLPRKNLRSILERIFEEDKS-CFQVSY---FPQTKVSRWFRNQNKK 333
                 ++  E      PRK ++ +   +   +K     V     FP  +V  WF ++   
Sbjct: 992  -----YKVEENSIESYPRKKIQLMSNALARYEKGLALDVLIGICFPGWQVPGWFNHRT-- 1051

Query: 334  DKVAIKLPLNIRKDKKWMGLAAFGIFSI--------SQNANCYCLSYEVQLDDTCLYRDH 393
              V ++L  N+ +     GLA   + ++          N      S E + +D  L+   
Sbjct: 1052 --VGLELKQNLPRHWNAGGLAGIALCAVVSFKDYISKNNRLLVTCSGEFKKEDKTLF--- 1111

Query: 394  IPMKLLEYSNRILILVFKPSDCYTYE-RNSSSNHVYISFNTNVERMQVESCGARLVYKDN 453
                      +   ++   ++  +YE R   S+HV+I + + +  M+           D+
Sbjct: 1112 ----------QFSCILGGWTEHGSYEAREIKSDHVFIGYTSWLNFMK----------SDD 1171

Query: 454  VKGLIQTIVDCIVESEDGLYEYYNQILMADLFSMINSSKHKVSSLESASVNKPATTTTAL 513
              G + T      +  DG  E  N  ++   FS+I S  +   SL + + +   TTT+  
Sbjct: 1172 SIGCVATEASLRFQVTDGTREVTNCTVVKCGFSLIYSHTNVDHSLCTDNYSDVTTTTSGG 1189

Query: 514  TDTDSSLLVEQLLYGSFPHPLLQK 518
            T + S   V +  Y +    ++++
Sbjct: 1232 TSSGSITDVGESCYQNGSEEIIEE 1189

BLAST of Spg021525 vs. TAIR 10
Match: AT1G27180.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 93.6 bits (231), Expect = 6.8e-19
Identity = 75/219 (34.25%), Postives = 100/219 (45.66%), Query Frame = 0

Query: 23   LESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRLLSLPTEIGNLRA 82
            L++ PK  G M+ L  L+L GSNI +L      L  L  L +++C  L  LP   G+L++
Sbjct: 1115 LKALPKTIGKMDTLYSLNLVGSNIEELPEEFGKLENLVELRMNNCKMLKRLPKSFGDLKS 1174

Query: 83   LKILILQCCTKLYQIPPSLANAESLEKL-------------NISGTSVS----HVPTSMH 142
            L  L +Q  T + ++P S  N  +L  L             N+ GTS       VP S  
Sbjct: 1175 LHRLYMQ-ETLVAELPESFGNLSNLMVLEMLKKPLFRISESNVPGTSEEPRFVEVPNSFS 1234

Query: 143  HLKNLIRL------VDEDVPKDLHCFSSLEELNLSYNEFIKLPDSLSHLKKLKRLYLNYC 202
             L  L  L      +   +P DL   S L +LNL  N F  LP SL  L  L+ L L  C
Sbjct: 1235 KLLKLEELDACSWRISGKIPDDLEKLSCLMKLNLGNNYFHSLPSSLVKLSNLQELSLRDC 1294

Query: 203  RKLKDLPKLPTSLENVVAVGCISISEKFYNKRLHIFSDL 219
            R+LK LP LP  LE +    C S+        L I +DL
Sbjct: 1295 RELKRLPPLPCKLEQLNLANCFSLESVSDLSELTILTDL 1332

BLAST of Spg021525 vs. TAIR 10
Match: AT5G45230.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 93.6 bits (231), Expect = 6.8e-19
Identity = 72/193 (37.31%), Postives = 98/193 (50.78%), Query Frame = 0

Query: 12  NLQTLALMSTGLESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRLL 71
           +L+TL L  +G  S        EHL  L+L G+ I  L P+I  L  L  LNL  C  L 
Sbjct: 728 SLKTLIL--SGCSSFQTFEVISEHLESLYLNGTEINGLPPAIGNLHRLIFLNLKDCKNLA 787

Query: 72  SLPTEIGNLRALKILILQCCTKLYQIPPSLANAESLEKLNISGTSVSHVPTSMHHLKNLI 131
           +LP  +G L++L+ L L  C+KL   P   A  ESL  L + GTS++ +P S+ HL    
Sbjct: 788 TLPDCLGELKSLQELKLSRCSKLKIFPDVTAKMESLLVLLLDGTSIAELPCSIFHL---- 847

Query: 132 RLVDEDVPKDLHCFSSLEELNLSYNEFIK-LPDSLSHLKKLKRLYLNYCRKLKDLPKLPT 191
                         SSL  L LS N+ I+ L   + H+  LK L L YC+ L  LP LP 
Sbjct: 848 --------------SSLRRLCLSRNDNIRTLRFDMGHMFHLKWLELKYCKNLTSLPILPP 900

Query: 192 SLENVVAVGCISI 204
           +L+ + A GC S+
Sbjct: 908 NLQCLNAHGCTSL 900

BLAST of Spg021525 vs. TAIR 10
Match: AT1G27170.1 (transmembrane receptors;ATP binding )

HSP 1 Score: 90.9 bits (224), Expect = 4.4e-18
Identity = 75/219 (34.25%), Postives = 100/219 (45.66%), Query Frame = 0

Query: 23   LESSPKIGGNMEHLTQLHLAGSNITQLHPSIECLTGLALLNLSHCTRLLSLPTEIGNLRA 82
            L+  PK  G+M+ L  L+L GSNI +L      L  L  L +S+C  L  LP   G+L++
Sbjct: 941  LKFLPKSIGDMDTLYSLNLEGSNIEELPEEFGKLEKLVELRMSNCKMLKRLPESFGDLKS 1000

Query: 83   LKILILQCCTKLYQIPPSLANAESLEKL-------------NISGTSVS----HVPTSMH 142
            L  L ++  T + ++P S  N  +L  L             N+ GTS       VP S  
Sbjct: 1001 LHRLYMK-ETLVSELPESFGNLSNLMVLEMLKKPLFRISESNVPGTSEEPRFVEVPNSFS 1060

Query: 143  HLKNLIRL------VDEDVPKDLHCFSSLEELNLSYNEFIKLPDSLSHLKKLKRLYLNYC 202
             L  L  L      +   +P DL   S L +LNL  N F  LP SL  L  L+ L L  C
Sbjct: 1061 KLLKLEELDACSWRISGKIPDDLEKLSCLMKLNLGNNYFHSLPSSLVKLSNLQELSLRDC 1120

Query: 203  RKLKDLPKLPTSLENVVAVGCISISEKFYNKRLHIFSDL 219
            R+LK LP LP  LE +    C S+        L I +DL
Sbjct: 1121 RELKRLPPLPCKLEQLNLANCFSLESVSDLSELTILTDL 1158

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890520.11.4e-18345.01TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890521.1 TMV... [more]
XP_038890531.11.4e-18345.01disease resistance-like protein DSC1 isoform X2 [Benincasa hispida][more]
KAA0039330.12.0e-18244.31TMV resistance protein N-like [Cucumis melo var. makuwa] >TYK00512.1 TMV resista... [more]
XP_011656060.24.5e-18243.77TMV resistance protein N isoform X1 [Cucumis sativus] >XP_011656061.2 TMV resist... [more]
XP_008459543.11.7e-18143.88PREDICTED: TMV resistance protein N-like [Cucumis melo] >XP_008459544.1 PREDICTE... [more]
Match NameE-valueIdentityDescription
V9M3981.4e-1633.14Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=... [more]
F4JT822.3e-1635.43Probable disease resistance protein At4g19520 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SZ664.0e-1633.48Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=... [more]
Q403923.4e-1525.34TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1[more]
V9M2S55.8e-1529.58Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A5A7T7V59.7e-18344.31TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S4E3628.2e-18243.88TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498648 PE=4 SV=1[more]
A0A6J1CL153.7e-10530.95TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012122 PE=... [more]
A0A6J1CJ314.2e-10131.38TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012055 PE=... [more]
A0A0A0KXU03.8e-8637.06TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G647530 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT1G69550.11.7e-2240.10disease resistance protein (TIR-NBS-LRR class) [more]
AT5G45200.11.1e-2124.80Disease resistance protein (TIR-NBS-LRR class) family [more]
AT1G27180.16.8e-1934.25disease resistance protein (TIR-NBS-LRR class), putative [more]
AT5G45230.16.8e-1937.31Disease resistance protein (TIR-NBS-LRR class) family [more]
AT1G27170.14.4e-1834.25transmembrane receptors;ATP binding [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 4..217
e-value: 3.0E-38
score: 133.1
IPR044974Disease resistance protein, plantsPANTHERPTHR11017LEUCINE-RICH REPEAT-CONTAINING PROTEINcoord: 45..129
IPR044974Disease resistance protein, plantsPANTHERPTHR11017LEUCINE-RICH REPEAT-CONTAINING PROTEINcoord: 133..347
IPR044974Disease resistance protein, plantsPANTHERPTHR11017LEUCINE-RICH REPEAT-CONTAINING PROTEINcoord: 509..685
NoneNo IPR availablePANTHERPTHR11017:SF434REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATEDcoord: 45..129
NoneNo IPR availablePANTHERPTHR11017:SF434REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATEDcoord: 509..685
coord: 133..347
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 9..244
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 147..169
score: 7.781261

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg021525.1Spg021525.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0005515 protein binding