Spg021481 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg021481
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	TMV resistance protein N-like
Location	scaffold9: 7064731 .. 7066578 (-)
RNA-Seq Expression	Spg021481
Synteny	Spg021481

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAACAGACACAGGTTAAGGTTTCTGTAATTGTTTATAATAATGCTCCAGTAATTCTTTTGATTAGTCAATCTTATACAGTCTTATCAGCGGGATCCTCCTGGAACTTCGGATTTTTGGGAATGTGTTTCAACTCCAACGAAGCTCTACCGATCATGCACTAAGTAATTGGTTATCTCTAACTCAACTACCGACGTCCACCAACTATCTACAGTCTAGTGTGAATATTTGTCACACAACTCCTCAATCATCCCTGAAACTAGGAGAATATATACATATCGACTGGCCAGACGGGCACATAATAAAATTAAGCACAACAAATAATTCAAGATGATAGTAATCAAATAGAATTTCCAGATTTTATAAAAATAACAATCACATAGTTCTTAACCCGTGCCTACAAGCAATTAGTTCATGATATAAAACATAAAAGACGTTACAATCTCATTAAGCACAACCATAATGCGAAATAAACAAGAAATACTAATCCTTCTTAGCAAATGGGGATGAAACGACTGCTCCAAACCACCAAGAACATGGAAGGACTTCGTCTATTTGAGTGGAATCGCAAATGGTCTTCTAATTCTCCATTCTACCGTCAATTCGGTCTCCAAATCGGTGATGCCCTTCATCTCCTACGGATCCTCCTTTTATAAGATGAGATTTAACCTAATTAGGTTAACTTATGTACGTGGAGTGATTTCCCGGCCTGATTTCTCCTAGATCTTCGCTTAGAAAACGCCACCTCATTTAATGAATCATGTGCAGCATACGTGGCTTGGTTTGCTAATATTCTACTGGATCATCGAACTTTGCTTTCTCAACTTGTTAACATTTATATTTTTCTGCAAAGAAACTCCAAAGTATCTTTATAAAGTTGTTCCTTTGGTTATGTAAAGCTCCATCGAGTAGCATTTTTGGGATCCCATCACTGGCTGGATTGTACTCTCTCACAAGACTAAACCTGAGGGATTGCAACCTCGAAGTAATCCCACAAGGGATCGAGTGTTTGCTATCACTGACAGAATTGAATCTATGCGGCAACAACTTCTCTCACCTTCCAACGAGCATTTCTCAACTTCATAACCTCAAAAGATTCAACATAAAACAATGCAAGAAGCTTCTACATTTCCCAGAGCTGCCACCCAGAATCTTGAGGCTGTTTTCCAAGGATTGCGTTTCGCTTAAAAGCATTCCAGACATTTCGAAAGTCGAGCATTTGTATTTCATGACAGAATTGAATCTTATGAATTTCTATCAATTGACTAACAACAAAGAACTTCATAGAATGGTAGTTTCTTGGATGGAGAAGATGTTGTTTCGAAAAGGAGCATTCAACATCATGATTCCCGGGAGTGAGATTCCATATTGGTTTACCACAAAAATGGGGTCTTACATAAGCGTCAAATGGGATCCAAAAGCACCAAATGCCCACTTGATTCACTTTGCTTGTGTGTTATGTGGTCCAATTGATGACCAAGACGACATGGCTGATGTTTCTGGCTCCATTGTTGCATCATTTACTGGAAAAAACCATGAGGACTCGAACTTGAAGAATGGAGATCTTTTAGTTAATGGATTCACTATTTCTGGGATGAGGAAGTTAGACCACATTTGGCTGTTTGTTTTGCCTCGGACAAGGTTACTCACAAGAAAGATTAACAAATGTAAAGAGATTGAGTTTCGATTCTTGTTCCAAGCTAATTATGGTCAATCTGTTACCCCCAATATCGAATTGAAAAAGTGTGGGGTAGGTTTGATAAATATGGAAAAGAATAAGGAAGCCATGAAATGTTATGCTTCTCGTATTATCTTGAAGAACAAGATGAGGTTAAGAACAATGTAA

mRNA sequence

ATGGAAACAGACACAGCTCCATCGAGTAGCATTTTTGGGATCCCATCACTGGCTGGATTGTACTCTCTCACAAGACTAAACCTGAGGGATTGCAACCTCGAAGTAATCCCACAAGGGATCGAGTGTTTGCTATCACTGACAGAATTGAATCTATGCGGCAACAACTTCTCTCACCTTCCAACGAGCATTTCTCAACTTCATAACCTCAAAAGATTCAACATAAAACAATGCAAGAAGCTTCTACATTTCCCAGAGCTGCCACCCAGAATCTTGAGGCTGTTTTCCAAGGATTGCGTTTCGCTTAAAAGCATTCCAGACATTTCGAAAGTCGAGCATTTGTATTTCATGACAGAATTGAATCTTATGAATTTCTATCAATTGACTAACAACAAAGAACTTCATAGAATGGTAGTTTCTTGGATGGAGAAGATGTTGTTTCGAAAAGGAGCATTCAACATCATGATTCCCGGGAGTGAGATTCCATATTGGTTTACCACAAAAATGGGGTCTTACATAAGCGTCAAATGGGATCCAAAAGCACCAAATGCCCACTTGATTCACTTTGCTTGTGTGTTATGTGGTCCAATTGATGACCAAGACGACATGGCTGATGTTTCTGGCTCCATTGTTGCATCATTTACTGGAAAAAACCATGAGGACTCGAACTTGAAGAATGGAGATCTTTTAGTTAATGGATTCACTATTTCTGGGATGAGGAAGTTAGACCACATTTGGCTGTTTGTTTTGCCTCGGACAAGGTTACTCACAAGAAAGATTAACAAATGTAAAGAGATTGAGTTTCGATTCTTGTTCCAAGCTAATTATGGTCAATCTGTTACCCCCAATATCGAATTGAAAAAGTGTGGGGTAGGTTTGATAAATATGGAAAAGAATAAGGAAGCCATGAAATGTTATGCTTCTCGTATTATCTTGAAGAACAAGATGAGGTTAAGAACAATGTAA

Coding sequence (CDS)

ATGGAAACAGACACAGCTCCATCGAGTAGCATTTTTGGGATCCCATCACTGGCTGGATTGTACTCTCTCACAAGACTAAACCTGAGGGATTGCAACCTCGAAGTAATCCCACAAGGGATCGAGTGTTTGCTATCACTGACAGAATTGAATCTATGCGGCAACAACTTCTCTCACCTTCCAACGAGCATTTCTCAACTTCATAACCTCAAAAGATTCAACATAAAACAATGCAAGAAGCTTCTACATTTCCCAGAGCTGCCACCCAGAATCTTGAGGCTGTTTTCCAAGGATTGCGTTTCGCTTAAAAGCATTCCAGACATTTCGAAAGTCGAGCATTTGTATTTCATGACAGAATTGAATCTTATGAATTTCTATCAATTGACTAACAACAAAGAACTTCATAGAATGGTAGTTTCTTGGATGGAGAAGATGTTGTTTCGAAAAGGAGCATTCAACATCATGATTCCCGGGAGTGAGATTCCATATTGGTTTACCACAAAAATGGGGTCTTACATAAGCGTCAAATGGGATCCAAAAGCACCAAATGCCCACTTGATTCACTTTGCTTGTGTGTTATGTGGTCCAATTGATGACCAAGACGACATGGCTGATGTTTCTGGCTCCATTGTTGCATCATTTACTGGAAAAAACCATGAGGACTCGAACTTGAAGAATGGAGATCTTTTAGTTAATGGATTCACTATTTCTGGGATGAGGAAGTTAGACCACATTTGGCTGTTTGTTTTGCCTCGGACAAGGTTACTCACAAGAAAGATTAACAAATGTAAAGAGATTGAGTTTCGATTCTTGTTCCAAGCTAATTATGGTCAATCTGTTACCCCCAATATCGAATTGAAAAAGTGTGGGGTAGGTTTGATAAATATGGAAAAGAATAAGGAAGCCATGAAATGTTATGCTTCTCGTATTATCTTGAAGAACAAGATGAGGTTAAGAACAATGTAA

Protein sequence

METDTAPSSSIFGIPSLAGLYSLTRLNLRDCNLEVIPQGIECLLSLTELNLCGNNFSHLPTSISQLHNLKRFNIKQCKKLLHFPELPPRILRLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFYQLTNNKELHRMVVSWMEKMLFRKGAFNIMIPGSEIPYWFTTKMGSYISVKWDPKAPNAHLIHFACVLCGPIDDQDDMADVSGSIVASFTGKNHEDSNLKNGDLLVNGFTISGMRKLDHIWLFVLPRTRLLTRKINKCKEIEFRFLFQANYGQSVTPNIELKKCGVGLINMEKNKEAMKCYASRIILKNKMRLRTM

Homology

[Close]

BLAST of Spg021481 vs. NCBI nr
Match: KAG6592347.1 (Disease resistance protein RUN1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 448.0 bits (1151), Expect = 7.0e-122
Identity = 217/313 (69.33%), Postives = 256/313 (81.79%), Query Frame = 0


Query: 6    APSSSIFGIPSLAGLYSLTRLNLRDCNLEVIPQGIECLLSLTELNLCGNNFSHLPTSISQ 65
            AP+S IFGIPSLAGLYSLTRLNL DC LE +P+GIECL+SL  LNL  NNFS LPTSIS+
Sbjct: 808  APTSGIFGIPSLAGLYSLTRLNLSDCKLEEVPEGIECLVSLVNLNLSRNNFSRLPTSISR 867

Query: 66   LHNLKRFNIKQCKKLLHFPELPPRILRLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFY 125
            LHNLKR N+ +C+KLLHFPELPPRILRL SK C+SLK   DISKV+H YFM E+NLMN +
Sbjct: 868  LHNLKRLNVNECEKLLHFPELPPRILRLMSKGCISLKDFLDISKVDHSYFMIEMNLMNCF 927

Query: 126  QLTNNKELHRMVVSWMEKMLFRKGAFNIMIPGSEIPYWFTT-KMGSYISVKWDPKAPNAH 185
            Q  +NKELH+++ SWM+KMLFRKGAFNI++PGSEIP WFTT KMGS I V+WD  APNA+
Sbjct: 928  QWVDNKELHKLITSWMQKMLFRKGAFNILVPGSEIPDWFTTKKMGSSICVEWDHDAPNAN 987

Query: 186  LIHFA-CVLCGPIDDQDDMADVSGSIVASFTGKNHEDSNLKNGDLLVNGFTISGMRKLDH 245
            ++ FA CV+CGP  ++ D+ DV  +I AS TGK+  D NL NGDL+V  F +SGM+KLDH
Sbjct: 988  MVRFALCVICGP-SNKKDILDVPFTIFASVTGKDRNDPNLNNGDLIVGAFNVSGMKKLDH 1047

Query: 246  IWLFVLPRTRLLTRKINKCKEIEFRFLFQANYGQSVTPNIELKKCGVGLINMEKNKEAMK 305
            IW+FVLPRT  LTRKI  CKEIEFRFL Q NY Q+V PN+ELKKCGVGLINME+  EAMK
Sbjct: 1048 IWMFVLPRTTTLTRKIRNCKEIEFRFLLQFNYNQTVIPNVELKKCGVGLINMEEESEAMK 1107

Query: 306  CYASRIILKNKMR 317
             YAS II+KN+M+
Sbjct: 1108 RYASYIIVKNRMK 1119

[Close]

BLAST of Spg021481 vs. NCBI nr
Match: XP_023535476.1 (TMV resistance protein N-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 446.4 bits (1147), Expect = 2.0e-121
Identity = 215/313 (68.69%), Postives = 255/313 (81.47%), Query Frame = 0


Query: 6    APSSSIFGIPSLAGLYSLTRLNLRDCNLEVIPQGIECLLSLTELNLCGNNFSHLPTSISQ 65
            AP+S IFGIPSLAGLYSLTRLNL DC LE +P+GIECL+SL  LNL  NNFSHLPTSIS+
Sbjct: 808  APTSGIFGIPSLAGLYSLTRLNLSDCKLEEVPEGIECLVSLVHLNLSRNNFSHLPTSISR 867

Query: 66   LHNLKRFNIKQCKKLLHFPELPPRILRLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFY 125
            LHNLKR N+ +C+KLLHFP+LPPRILRL SK C+SLK   DISKV+H YFM E+NLMN +
Sbjct: 868  LHNLKRLNVNECEKLLHFPKLPPRILRLMSKGCISLKDFLDISKVDHSYFMIEMNLMNCF 927

Query: 126  QLTNNKELHRMVVSWMEKMLFRKGAFNIMIPGSEIPYWFTT-KMGSYISVKWDPKAPNAH 185
            Q  +NKELH+++ SWM+KMLFRKG FNI++PG EIP WFTT KMGS I V+WD  APNA+
Sbjct: 928  QWVDNKELHKLITSWMQKMLFRKGPFNILVPGCEIPDWFTTKKMGSSICVEWDHDAPNAN 987

Query: 186  LIHFA-CVLCGPIDDQDDMADVSGSIVASFTGKNHEDSNLKNGDLLVNGFTISGMRKLDH 245
            ++ FA CV+CGP  ++ D+ DV   I AS TGK+H D NL NGDL+V  F +SGM+KLDH
Sbjct: 988  MVRFALCVICGP-SNKKDIIDVPFIIFASVTGKDHNDPNLNNGDLIVGAFNVSGMKKLDH 1047

Query: 246  IWLFVLPRTRLLTRKINKCKEIEFRFLFQANYGQSVTPNIELKKCGVGLINMEKNKEAMK 305
            IW+FVLPRT  LTRKI  CKEIEFRF+ Q NY Q+V PN+ELKKCGVGLINME+  EAMK
Sbjct: 1048 IWMFVLPRTTTLTRKIRNCKEIEFRFVLQFNYNQTVIPNVELKKCGVGLINMEEESEAMK 1107

Query: 306  CYASRIILKNKMR 317
             YAS II+KN+M+
Sbjct: 1108 RYASYIIVKNRMK 1119

[Close]

BLAST of Spg021481 vs. NCBI nr
Match: XP_022925367.1 (TMV resistance protein N-like [Cucurbita moschata])

HSP 1 Score: 444.9 bits (1143), Expect = 5.9e-121
Identity = 216/313 (69.01%), Postives = 255/313 (81.47%), Query Frame = 0


Query: 6    APSSSIFGIPSLAGLYSLTRLNLRDCNLEVIPQGIECLLSLTELNLCGNNFSHLPTSISQ 65
            AP+S IFGIPSLAGLYSLTRLNL DC LE +P+GIECL+SL  LNL  NNFS LPTSIS+
Sbjct: 808  APTSGIFGIPSLAGLYSLTRLNLSDCKLEEVPEGIECLVSLVNLNLSRNNFSRLPTSISR 867

Query: 66   LHNLKRFNIKQCKKLLHFPELPPRILRLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFY 125
            LHNLKR N+ +C+KLLHFPELPPRILRL SK C+SLK   DISKV+H YFM E+NLMN +
Sbjct: 868  LHNLKRLNVNECEKLLHFPELPPRILRLMSKGCISLKDFLDISKVDHSYFMIEMNLMNCF 927

Query: 126  QLTNNKELHRMVVSWMEKMLFRKGAFNIMIPGSEIPYWFTT-KMGSYISVKWDPKAPNAH 185
            Q  +NKEL +++ SWM+KMLFRKGAFNI++PGSEIP WFTT KMGS I V+WD  APNA+
Sbjct: 928  QWVDNKELQKLITSWMQKMLFRKGAFNILVPGSEIPDWFTTRKMGSSICVEWDHDAPNAN 987

Query: 186  LIHFA-CVLCGPIDDQDDMADVSGSIVASFTGKNHEDSNLKNGDLLVNGFTISGMRKLDH 245
            ++ FA CV+CGP  ++ D+ DV  +I AS TGK+  D NL NGDL+V  F +SGM+KLDH
Sbjct: 988  MVRFALCVICGP-SNKKDILDVPFTIFASVTGKDRNDPNLNNGDLIVGAFNVSGMKKLDH 1047

Query: 246  IWLFVLPRTRLLTRKINKCKEIEFRFLFQANYGQSVTPNIELKKCGVGLINMEKNKEAMK 305
            IW+FVLPRT  LTRKI  CKEIEFRFL Q NY Q+V PN+ELKKCGVGLINME+  EAMK
Sbjct: 1048 IWMFVLPRTTTLTRKIRNCKEIEFRFLLQFNYNQTVIPNVELKKCGVGLINMEEESEAMK 1107

Query: 306  CYASRIILKNKMR 317
             YAS II+KN+M+
Sbjct: 1108 RYASYIIVKNRMK 1119

[Close]

BLAST of Spg021481 vs. NCBI nr
Match: XP_022973476.1 (TMV resistance protein N-like [Cucurbita maxima])

HSP 1 Score: 444.5 bits (1142), Expect = 7.7e-121
Identity = 215/313 (68.69%), Postives = 255/313 (81.47%), Query Frame = 0


Query: 6    APSSSIFGIPSLAGLYSLTRLNLRDCNLEVIPQGIECLLSLTELNLCGNNFSHLPTSISQ 65
            AP+S IFGIPSLAGLYSLTRLNL DC LE +P+GIECL+SL  LNL  NNFS LPTSIS+
Sbjct: 808  APTSGIFGIPSLAGLYSLTRLNLSDCKLEEVPEGIECLVSLVNLNLSRNNFSRLPTSISR 867

Query: 66   LHNLKRFNIKQCKKLLHFPELPPRILRLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFY 125
            LHNLKR N+ +C+KLLHFP+LPPRILRL SK C+SLK   DISKV+H YFM E+NL+N +
Sbjct: 868  LHNLKRLNVNECEKLLHFPKLPPRILRLMSKGCISLKDFLDISKVDHSYFMIEMNLLNCF 927

Query: 126  QLTNNKELHRMVVSWMEKMLFRKGAFNIMIPGSEIPYWFTT-KMGSYISVKWDPKAPNAH 185
            Q  +NK+LH+++ SWM+KMLFRKGAFNI++PGSEIP WFTT KMGS I V+WD  APNA+
Sbjct: 928  QWVDNKDLHKLITSWMQKMLFRKGAFNILVPGSEIPDWFTTKKMGSSICVEWDHDAPNAN 987

Query: 186  LIHFA-CVLCGPIDDQDDMADVSGSIVASFTGKNHEDSNLKNGDLLVNGFTISGMRKLDH 245
            ++ FA CV+CGP + Q D+ DV  +I AS TGKN  D N  NGDL+V  F +SGM+KLDH
Sbjct: 988  MVRFALCVICGPSNKQ-DILDVPVTIFASVTGKNRNDPNSNNGDLIVGAFNVSGMKKLDH 1047

Query: 246  IWLFVLPRTRLLTRKINKCKEIEFRFLFQANYGQSVTPNIELKKCGVGLINMEKNKEAMK 305
            IW+FVLPRT  LTRKI  CKEIEFRFL Q NY Q+V PN+ELKKCGVGLINME+  EAMK
Sbjct: 1048 IWMFVLPRTTTLTRKIRNCKEIEFRFLLQFNYNQTVIPNVELKKCGVGLINMEEESEAMK 1107

Query: 306  CYASRIILKNKMR 317
             YAS II+KN+M+
Sbjct: 1108 RYASYIIVKNRMK 1119

[Close]

BLAST of Spg021481 vs. NCBI nr
Match: XP_022141876.1 (TMV resistance protein N-like isoform X1 [Momordica charantia])

HSP 1 Score: 441.0 bits (1133), Expect = 8.5e-120
Identity = 221/314 (70.38%), Postives = 257/314 (81.85%), Query Frame = 0


Query: 6    APSSSIFGIPSLAGLYSLTRLNLRDCNLEVIPQGIECLLSLTELNLCGNNFSHLPTSISQ 65
            AP S IFG+PSLAGLYSLT+LNL+DCNLE IPQGIECL+SLTEL LCGNNFSHLPTSIS+
Sbjct: 831  APESGIFGVPSLAGLYSLTKLNLKDCNLETIPQGIECLVSLTELRLCGNNFSHLPTSISR 890

Query: 66   LHNLKRFNIKQCKKLLHFPELPPRILRLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFY 125
            LHNL+R NI  CKKLL FPELPPRI  L SK C+SLK+IP+ISKV+H YFM E+NL+N Y
Sbjct: 891  LHNLRRLNINGCKKLLCFPELPPRIRWLMSKGCISLKNIPNISKVDHSYFMREVNLLNCY 950

Query: 126  QLTNNKELHRMVVSWMEKMLFRKGAFNIMIPGSEIPYWFT-TKMGSYISVKWDPKAPNAH 185
            QL +N  LHR++  WMEKMLFRKG FNIMIPGS+IP WFT T+MGS I V+WDP A NA+
Sbjct: 951  QLADNIGLHRLITYWMEKMLFRKGTFNIMIPGSQIPDWFTMTEMGSSICVEWDPDAANAN 1010

Query: 186  LIHFA-CVLCGPIDDQDDMADVSGSIVASFTGKNHEDSNLKNGDLLVNGFTISGMRKLDH 245
            LI FA CV+CGP  +Q D+ D   +I+AS TG+  +D  L NGDL+VN F +SGMRKLDH
Sbjct: 1011 LIRFALCVVCGP-TNQGDIIDTPFAIIASVTGRGADDPCLNNGDLIVNAFNVSGMRKLDH 1070

Query: 246  IWLFVLPRTRLLTRKINKCKEIEFRFLFQANYGQSV-TPNIELKKCGVGLINMEKNKEAM 305
            IWLFVLPRT+ L RKI+ CKEI+F+FL Q NY +S  +P+I LKKCGV LINME+ KEAM
Sbjct: 1071 IWLFVLPRTKSLARKISTCKEIKFQFLVQVNYSRSASSPSIALKKCGVHLINMEEEKEAM 1130

Query: 306  KCYASRIILKNKMR 317
            K YAS IILKNKMR
Sbjct: 1131 KRYASYIILKNKMR 1143

[Close]

BLAST of Spg021481 vs. ExPASy Swiss-Prot
Match: Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.2e-15
Identity = 85/339 (25.07%), Postives = 146/339 (43.07%), Query Frame = 0


Query: 3    TDTAPSSSIFGIPSLAGLYSLTRLNLRDCNLEVIPQGIECLLSLTELNLCGNNFSHLPTS 62
            T +  S S+F +P   G   LT L L  C+L  +P  I  L SL  L L GNN  +LP S
Sbjct: 837  TSSHVSVSMFFMPPTLGCSRLTDLYLSRCSLYKLPDNIGGLSSLQSLCLSGNNIENLPES 896

Query: 63   ISQLHNLKRFNIKQCKKLLHFPELPPRILRLFSKDCVSLKSIPD----ISKVEHLYFMTE 122
             +QL+NLK F++K CK L   P LP  +  L + +C SL+++ +    ++  E ++ M  
Sbjct: 897  FNQLNNLKWFDLKFCKMLKSLPVLPQNLQYLDAHECESLETLANPLTPLTVGERIHSM-- 956

Query: 123  LNLMNFYQLTNNKEL---------HRMVVSWMEKMLFR----KGAFNIMIPGSEIPYWFT 182
                N Y+L  + +           +++ +   K  +R    +    I  P +EIP WF 
Sbjct: 957  FIFSNCYKLNQDAQASLVGHARIKSQLMANASAKRYYRGFVPEPLVGICYPATEIPSWFC 1016

Query: 183  -TKMGSYISVKWDPKAPNAHLIHFACVLCGPIDDQDDMAD-VSGSIVASFTGKNHEDSNL 242
              ++G  + +   P   + + +  A  +     D +D A   S     +F  K   DS+ 
Sbjct: 1017 HQRLGRSLEIPLPPHWCDINFVGLALSVVVSFKDYEDSAKRFSVKCCGNFENK---DSSF 1076

Query: 243  KNGDLLVNGFT------ISGMRKLDHIWLFVLPRTRLLTRKI----NKC--KEIEFRFLF 302
               D  + G+           RKL    +F+   +  L + +    N C   +  F F  
Sbjct: 1077 TRFDFTLAGWNEPCGSLSHESRKLTSDHVFMGYNSCFLVKNVHGESNSCCYTKASFEFYV 1136

Query: 303  QANYGQSVTPNIELKKCGVGLINMEKNKEAMKCYASRII 311
              +  +      E+ KCG+ L+ + ++ + M    + I+
Sbjct: 1137 TDDETRKKIETCEVIKCGMSLMYVPEDDDCMLLKKTNIV 1170

[Close]

BLAST of Spg021481 vs. ExPASy Swiss-Prot
Match: Q9SCX7 (Inactive disease resistance protein RPS4 OS=Arabidopsis thaliana OX=3702 GN=RPS4 PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.0e-11
Identity = 82/289 (28.37%), Postives = 124/289 (42.91%), Query Frame = 0


Query: 33   LEVIPQGIECLLSLTELNLCGN-NFSHLPTSISQLHNLKRFNIKQCKKLLHFPELPPRIL 92
            +EV+PQ    L S+  L L  N   S LP  ISQL  LK  ++K C  L   PE PP + 
Sbjct: 831  IEVMPQ----LPSVQYLCLSRNAKISCLPVGISQLSQLKWLDLKYCTSLTSVPEFPPNLQ 890

Query: 93   RLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFY-------------QLTNNKELHRMVV 152
             L +  C SLK+   +SK       TE N   F              ++T+  +    ++
Sbjct: 891  CLDAHGCSSLKT---VSKPLARIMPTEQNHSTFIFTNCENLEQAAKEEITSYAQRKCQLL 950

Query: 153  SWMEKM----LFRKGAFNIMIPGSEIPYWFTTK-MGSYISVKWDPKAPNAHLIHFA-CVL 212
            S+  K     L  +  F+   PG E+P WF  + +GS + VK  P   +  L   A C +
Sbjct: 951  SYARKRHNGGLVSESLFSTCFPGCEVPSWFCHETVGSELEVKLLPHWHDKKLAGIALCAV 1010

Query: 213  CGPIDDQDDMADVSGSIVASFTGKNHEDSNLKNGDLLVNGFTISGMRK----LDHIWL-- 272
               +D QD ++ +  S+  +F  K+ ED +       V  +T  G  K    LDH+++  
Sbjct: 1011 VSCLDPQDQVSRL--SVTCTFKVKD-EDKSWVPYTCPVGSWTRHGGGKDKIELDHVFIGY 1070

Query: 273  FVLPRTRLLTRKIN--KCKEIEFRFLFQANYGQSVTPNIELKKCGVGLI 294
               P T     + N  +C   E    F    G S     ++ KCG+ L+
Sbjct: 1071 TSCPHTIKCHEEGNSDECNPTEASLKFTVTGGTSENGKYKVLKCGLSLV 1109

[Close]

BLAST of Spg021481 vs. ExPASy Swiss-Prot
Match: C4B7M7 (Disease resistance protein RPS4 OS=Arabidopsis thaliana OX=3702 GN=RPS4 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.0e-11
Identity = 82/289 (28.37%), Postives = 124/289 (42.91%), Query Frame = 0


Query: 33   LEVIPQGIECLLSLTELNLCGN-NFSHLPTSISQLHNLKRFNIKQCKKLLHFPELPPRIL 92
            +EV+PQ    L S+  L L  N   S LP  ISQL  LK  ++K C  L   PE PP + 
Sbjct: 831  IEVMPQ----LPSVQYLCLSRNAKISCLPVGISQLSQLKWLDLKYCTSLTSVPEFPPNLQ 890

Query: 93   RLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFY-------------QLTNNKELHRMVV 152
             L +  C SLK+   +SK       TE N   F              ++T+  +    ++
Sbjct: 891  CLDAHGCSSLKT---VSKPLARIMPTEQNHSTFIFTNCENLEQAAKEEITSYAQRKCQLL 950

Query: 153  SWMEKM----LFRKGAFNIMIPGSEIPYWFTTK-MGSYISVKWDPKAPNAHLIHFA-CVL 212
            S+  K     L  +  F+   PG E+P WF  + +GS + VK  P   +  L   A C +
Sbjct: 951  SYARKRYNGGLVSESLFSTCFPGCEVPSWFCHETVGSELEVKLLPHWHDKKLAGIALCAV 1010

Query: 213  CGPIDDQDDMADVSGSIVASFTGKNHEDSNLKNGDLLVNGFTISGMRK----LDHIWL-- 272
               +D QD ++ +  S+  +F  K+ ED +       V  +T  G  K    LDH+++  
Sbjct: 1011 VSCLDPQDQVSRL--SVTCTFKVKD-EDKSWVPYTCPVGSWTRHGGGKDKIELDHVFIGY 1070

Query: 273  FVLPRTRLLTRKIN--KCKEIEFRFLFQANYGQSVTPNIELKKCGVGLI 294
               P T     + N  +C   E    F    G S     ++ KCG+ L+
Sbjct: 1071 TSCPHTIKCHEEGNSDECNPTEASLKFTVTGGTSENGKYKVLKCGLSLV 1109

[Close]

BLAST of Spg021481 vs. ExPASy Swiss-Prot
Match: Q9XGM3 (Disease resistance protein RPS4 OS=Arabidopsis thaliana OX=3702 GN=RPS4 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.0e-11
Identity = 82/289 (28.37%), Postives = 124/289 (42.91%), Query Frame = 0


Query: 33   LEVIPQGIECLLSLTELNLCGN-NFSHLPTSISQLHNLKRFNIKQCKKLLHFPELPPRIL 92
            +EV+PQ    L S+  L L  N   S LP  ISQL  LK  ++K C  L   PE PP + 
Sbjct: 831  IEVMPQ----LPSVQYLCLSRNAKISCLPVGISQLSQLKWLDLKYCTSLTSVPEFPPNLQ 890

Query: 93   RLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFY-------------QLTNNKELHRMVV 152
             L +  C SLK+   +SK       TE N   F              ++T+  +    ++
Sbjct: 891  CLDAHGCSSLKT---VSKPLARIMPTEQNHSTFIFTNCENLEQAAKEEITSYAQRKCQLL 950

Query: 153  SWMEKM----LFRKGAFNIMIPGSEIPYWFTTK-MGSYISVKWDPKAPNAHLIHFA-CVL 212
            S+  K     L  +  F+   PG E+P WF  + +GS + VK  P   +  L   A C +
Sbjct: 951  SYARKRYNGGLVSESLFSTCFPGCEVPSWFCHETVGSELEVKLLPHWHDKKLAGIALCAV 1010

Query: 213  CGPIDDQDDMADVSGSIVASFTGKNHEDSNLKNGDLLVNGFTISGMRK----LDHIWL-- 272
               +D QD ++ +  S+  +F  K+ ED +       V  +T  G  K    LDH+++  
Sbjct: 1011 VSCLDPQDQVSRL--SVTCTFKVKD-EDKSWVAYTCPVGSWTRHGGGKDKIELDHVFIGY 1070

Query: 273  FVLPRTRLLTRKIN--KCKEIEFRFLFQANYGQSVTPNIELKKCGVGLI 294
               P T     + N  +C   E    F    G S     ++ KCG+ L+
Sbjct: 1071 TSCPHTIKCHEEGNSDECNPTEASLKFTVTGGTSENGKYKVLKCGLSLV 1109

[Close]

BLAST of Spg021481 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.0e-11
Identity = 47/124 (37.90%), Postives = 65/124 (52.42%), Query Frame = 0


Query: 15  PSLAGLYSLTRLNLRDCNL--EVIPQGIECLLSLTELNLCGNNFSHLPTSISQLHNLKRF 74
           P   GL+SL  LNL  CNL    +P+ I  L SL +L+L  NNF HLP+SI+QL  L+  
Sbjct: 827 PVAEGLHSLEYLNLSYCNLIDGGLPEEIGSLSSLKKLDLSRNNFEHLPSSIAQLGALQSL 886

Query: 75  NIKQCKKLLHFPELPPRILRLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFYQLTNNKE 134
           ++K C++L   PELPP +  L     ++LK I                    Y +T  K+
Sbjct: 887 DLKDCQRLTQLPELPPELNELHVDCHMALKFI-------------------HYLVTKRKK 931

Query: 135 LHRM 137
           LHR+
Sbjct: 947 LHRV 931

[Close]

BLAST of Spg021481 vs. ExPASy TrEMBL
Match: A0A6J1EBI7 (TMV resistance protein N-like OS=Cucurbita moschata OX=3662 GN=LOC111432675 PE=4 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 2.9e-121
Identity = 216/313 (69.01%), Postives = 255/313 (81.47%), Query Frame = 0


Query: 6    APSSSIFGIPSLAGLYSLTRLNLRDCNLEVIPQGIECLLSLTELNLCGNNFSHLPTSISQ 65
            AP+S IFGIPSLAGLYSLTRLNL DC LE +P+GIECL+SL  LNL  NNFS LPTSIS+
Sbjct: 808  APTSGIFGIPSLAGLYSLTRLNLSDCKLEEVPEGIECLVSLVNLNLSRNNFSRLPTSISR 867

Query: 66   LHNLKRFNIKQCKKLLHFPELPPRILRLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFY 125
            LHNLKR N+ +C+KLLHFPELPPRILRL SK C+SLK   DISKV+H YFM E+NLMN +
Sbjct: 868  LHNLKRLNVNECEKLLHFPELPPRILRLMSKGCISLKDFLDISKVDHSYFMIEMNLMNCF 927

Query: 126  QLTNNKELHRMVVSWMEKMLFRKGAFNIMIPGSEIPYWFTT-KMGSYISVKWDPKAPNAH 185
            Q  +NKEL +++ SWM+KMLFRKGAFNI++PGSEIP WFTT KMGS I V+WD  APNA+
Sbjct: 928  QWVDNKELQKLITSWMQKMLFRKGAFNILVPGSEIPDWFTTRKMGSSICVEWDHDAPNAN 987

Query: 186  LIHFA-CVLCGPIDDQDDMADVSGSIVASFTGKNHEDSNLKNGDLLVNGFTISGMRKLDH 245
            ++ FA CV+CGP  ++ D+ DV  +I AS TGK+  D NL NGDL+V  F +SGM+KLDH
Sbjct: 988  MVRFALCVICGP-SNKKDILDVPFTIFASVTGKDRNDPNLNNGDLIVGAFNVSGMKKLDH 1047

Query: 246  IWLFVLPRTRLLTRKINKCKEIEFRFLFQANYGQSVTPNIELKKCGVGLINMEKNKEAMK 305
            IW+FVLPRT  LTRKI  CKEIEFRFL Q NY Q+V PN+ELKKCGVGLINME+  EAMK
Sbjct: 1048 IWMFVLPRTTTLTRKIRNCKEIEFRFLLQFNYNQTVIPNVELKKCGVGLINMEEESEAMK 1107

Query: 306  CYASRIILKNKMR 317
             YAS II+KN+M+
Sbjct: 1108 RYASYIIVKNRMK 1119

[Close]

BLAST of Spg021481 vs. ExPASy TrEMBL
Match: A0A6J1I8R5 (TMV resistance protein N-like OS=Cucurbita maxima OX=3661 GN=LOC111472025 PE=4 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 3.7e-121
Identity = 215/313 (68.69%), Postives = 255/313 (81.47%), Query Frame = 0


Query: 6    APSSSIFGIPSLAGLYSLTRLNLRDCNLEVIPQGIECLLSLTELNLCGNNFSHLPTSISQ 65
            AP+S IFGIPSLAGLYSLTRLNL DC LE +P+GIECL+SL  LNL  NNFS LPTSIS+
Sbjct: 808  APTSGIFGIPSLAGLYSLTRLNLSDCKLEEVPEGIECLVSLVNLNLSRNNFSRLPTSISR 867

Query: 66   LHNLKRFNIKQCKKLLHFPELPPRILRLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFY 125
            LHNLKR N+ +C+KLLHFP+LPPRILRL SK C+SLK   DISKV+H YFM E+NL+N +
Sbjct: 868  LHNLKRLNVNECEKLLHFPKLPPRILRLMSKGCISLKDFLDISKVDHSYFMIEMNLLNCF 927

Query: 126  QLTNNKELHRMVVSWMEKMLFRKGAFNIMIPGSEIPYWFTT-KMGSYISVKWDPKAPNAH 185
            Q  +NK+LH+++ SWM+KMLFRKGAFNI++PGSEIP WFTT KMGS I V+WD  APNA+
Sbjct: 928  QWVDNKDLHKLITSWMQKMLFRKGAFNILVPGSEIPDWFTTKKMGSSICVEWDHDAPNAN 987

Query: 186  LIHFA-CVLCGPIDDQDDMADVSGSIVASFTGKNHEDSNLKNGDLLVNGFTISGMRKLDH 245
            ++ FA CV+CGP + Q D+ DV  +I AS TGKN  D N  NGDL+V  F +SGM+KLDH
Sbjct: 988  MVRFALCVICGPSNKQ-DILDVPVTIFASVTGKNRNDPNSNNGDLIVGAFNVSGMKKLDH 1047

Query: 246  IWLFVLPRTRLLTRKINKCKEIEFRFLFQANYGQSVTPNIELKKCGVGLINMEKNKEAMK 305
            IW+FVLPRT  LTRKI  CKEIEFRFL Q NY Q+V PN+ELKKCGVGLINME+  EAMK
Sbjct: 1048 IWMFVLPRTTTLTRKIRNCKEIEFRFLLQFNYNQTVIPNVELKKCGVGLINMEEESEAMK 1107

Query: 306  CYASRIILKNKMR 317
             YAS II+KN+M+
Sbjct: 1108 RYASYIIVKNRMK 1119

[Close]

BLAST of Spg021481 vs. ExPASy TrEMBL
Match: A0A6J1CKI7 (TMV resistance protein N-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012132 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 4.1e-120
Identity = 221/314 (70.38%), Postives = 257/314 (81.85%), Query Frame = 0


Query: 6    APSSSIFGIPSLAGLYSLTRLNLRDCNLEVIPQGIECLLSLTELNLCGNNFSHLPTSISQ 65
            AP S IFG+PSLAGLYSLT+LNL+DCNLE IPQGIECL+SLTEL LCGNNFSHLPTSIS+
Sbjct: 831  APESGIFGVPSLAGLYSLTKLNLKDCNLETIPQGIECLVSLTELRLCGNNFSHLPTSISR 890

Query: 66   LHNLKRFNIKQCKKLLHFPELPPRILRLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFY 125
            LHNL+R NI  CKKLL FPELPPRI  L SK C+SLK+IP+ISKV+H YFM E+NL+N Y
Sbjct: 891  LHNLRRLNINGCKKLLCFPELPPRIRWLMSKGCISLKNIPNISKVDHSYFMREVNLLNCY 950

Query: 126  QLTNNKELHRMVVSWMEKMLFRKGAFNIMIPGSEIPYWFT-TKMGSYISVKWDPKAPNAH 185
            QL +N  LHR++  WMEKMLFRKG FNIMIPGS+IP WFT T+MGS I V+WDP A NA+
Sbjct: 951  QLADNIGLHRLITYWMEKMLFRKGTFNIMIPGSQIPDWFTMTEMGSSICVEWDPDAANAN 1010

Query: 186  LIHFA-CVLCGPIDDQDDMADVSGSIVASFTGKNHEDSNLKNGDLLVNGFTISGMRKLDH 245
            LI FA CV+CGP  +Q D+ D   +I+AS TG+  +D  L NGDL+VN F +SGMRKLDH
Sbjct: 1011 LIRFALCVVCGP-TNQGDIIDTPFAIIASVTGRGADDPCLNNGDLIVNAFNVSGMRKLDH 1070

Query: 246  IWLFVLPRTRLLTRKINKCKEIEFRFLFQANYGQSV-TPNIELKKCGVGLINMEKNKEAM 305
            IWLFVLPRT+ L RKI+ CKEI+F+FL Q NY +S  +P+I LKKCGV LINME+ KEAM
Sbjct: 1071 IWLFVLPRTKSLARKISTCKEIKFQFLVQVNYSRSASSPSIALKKCGVHLINMEEEKEAM 1130

Query: 306  KCYASRIILKNKMR 317
            K YAS IILKNKMR
Sbjct: 1131 KRYASYIILKNKMR 1143

[Close]

BLAST of Spg021481 vs. ExPASy TrEMBL
Match: M4R4K0 (Resistance gene-like protein OS=Cucumis melo OX=3656 GN=RGH14 PE=4 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 1.8e-115
Identity = 211/312 (67.63%), Postives = 255/312 (81.73%), Query Frame = 0


Query: 7   PSSSIFGIPSLAGLYSLTRLNLRDCNLEVIPQGIECLLSLTELNLCGNNFSHLPTSISQL 66
           P+S IFGIPSLAGLYSLT+LNLRDCNLE IPQGIECL+SL EL+L  N+FSHLPTSIS+L
Sbjct: 598 PASGIFGIPSLAGLYSLTKLNLRDCNLEEIPQGIECLVSLVELDLSDNSFSHLPTSISRL 657

Query: 67  HNLKRFNIKQCKKLLHFPELPPRILRLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFYQ 126
           HNLK+  I QCKKL+ FP+LPPRIL L SKDC+SLK   DISKV++LY M E+NL+N YQ
Sbjct: 658 HNLKKLRINQCKKLVQFPKLPPRILFLMSKDCISLKDFVDISKVDNLYIMKEVNLLNCYQ 717

Query: 127 LTNNKELHRMVVSWMEKMLFRKGAFNIMIPGSEIPYWFTT-KMGSYISVKWDPKAPNAHL 186
           L NNK  HR+++SWM+KMLFRKG FNIMIPGSEIP WFTT KMGS + ++WDP  PN ++
Sbjct: 718 LANNKGFHRLIISWMQKMLFRKGTFNIMIPGSEIPDWFTTRKMGSSVCIEWDPDGPNTNM 777

Query: 187 IHFA-CVLCGPIDDQDDMADVSGSIVASFTGKNHEDSNLKN-GDLLVNGFTISGMRKLDH 246
           I FA CV+ G  +  D +   S +I+AS TGK+  DSNLKN GDLL+ GF ++GM+KLDH
Sbjct: 778 IRFALCVVFGLSEKIDVVNVPSFAIIASVTGKDRNDSNLKNGGDLLIGGFPVAGMKKLDH 837

Query: 247 IWLFVLPRTRLLTRKINKCKEIEFRFLFQ-ANYGQSVTPNIELKKCGVGLINMEKNKEAM 306
           IW+FVLPRT  L RKI+  KEI+FRFL Q ANY QS+TPN+++K+CGVGLIN+E+ KEAM
Sbjct: 838 IWMFVLPRTGTLVRKISNYKEIKFRFLLQAANYRQSITPNVKVKECGVGLINLEEEKEAM 897

Query: 307 KCYASRIILKNK 315
           K YAS IIL+NK
Sbjct: 898 KRYASHIILRNK 909

[Close]

BLAST of Spg021481 vs. ExPASy TrEMBL
Match: A0A1S3CBN9 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498654 PE=4 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 2.3e-115
Identity = 211/312 (67.63%), Postives = 255/312 (81.73%), Query Frame = 0


Query: 7    PSSSIFGIPSLAGLYSLTRLNLRDCNLEVIPQGIECLLSLTELNLCGNNFSHLPTSISQL 66
            P+S IFGIPSLAGLYSLT+LNLRDCNLE IPQGIECL+SL EL+L GN+FSHLPTSIS+L
Sbjct: 826  PASGIFGIPSLAGLYSLTKLNLRDCNLEEIPQGIECLVSLVELDLSGNSFSHLPTSISRL 885

Query: 67   HNLKRFNIKQCKKLLHFPELPPRILRLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFYQ 126
            HNLK+  I QC KL+ FP+LPPRIL L SKDC+SLK   DISKV++LY M E+NL+N YQ
Sbjct: 886  HNLKKLRINQCIKLVQFPKLPPRILFLMSKDCISLKDFVDISKVDNLYIMKEVNLLNCYQ 945

Query: 127  LTNNKELHRMVVSWMEKMLFRKGAFNIMIPGSEIPYWFTT-KMGSYISVKWDPKAPNAHL 186
            L NNK  HR+++SWM+KMLFRKG FNIMIPGSEIP WFTT KMGS + ++WDP  PN ++
Sbjct: 946  LANNKGFHRLIISWMQKMLFRKGTFNIMIPGSEIPDWFTTRKMGSSVCIEWDPDGPNTNM 1005

Query: 187  IHFA-CVLCGPIDDQDDMADVSGSIVASFTGKNHEDSNLKN-GDLLVNGFTISGMRKLDH 246
            I FA CV+ G  +  D +   S +I+AS TGK+  DSNLKN GDLL+ GF ++GM+KLDH
Sbjct: 1006 IRFALCVVFGLSEKIDVVNVPSFAIIASVTGKDRNDSNLKNGGDLLIGGFPVAGMKKLDH 1065

Query: 247  IWLFVLPRTRLLTRKINKCKEIEFRFLFQ-ANYGQSVTPNIELKKCGVGLINMEKNKEAM 306
            IW+FVLPRT  L RKI+  KEI+FRFL Q ANY QS+TPN+++K+CGVGLIN+E+ KEAM
Sbjct: 1066 IWMFVLPRTGTLVRKISNYKEIKFRFLLQAANYRQSITPNVKVKECGVGLINLEEEKEAM 1125

Query: 307  KCYASRIILKNK 315
            K YAS IIL+NK
Sbjct: 1126 KRYASHIILRNK 1137

[Close]

BLAST of Spg021481 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 87.0 bits (214), Expect = 2.9e-17
Identity = 90/299 (30.10%), Postives = 139/299 (46.49%), Query Frame = 0


Query: 15   PSLAGLYSLTRLNLRDCNLEVIPQGIECLLSLTELNLCGNNFSHLPTSISQLHNLKRFNI 74
            P L+    L  L+L + N+  IP  I  L +L EL+L GNNF  +P SI +L  L R N+
Sbjct: 949  PPLSRFDDLRALSLSNMNMTEIPNSIGNLWNLLELDLSGNNFEFIPASIKRLTRLNRLNL 1008

Query: 75   KQCKKLLHFP-ELPPRILRLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFYQLTNNKE- 134
              C++L   P ELP  +L ++   C SL S   IS   + Y + +L   N Y+L    + 
Sbjct: 1009 NNCQRLQALPDELPRGLLYIYIHSCTSLVS---ISGCFNQYCLRKLVASNCYKLDQAAQI 1068

Query: 135  -LHRMVVSWMEKMLFRKGAFNIMIPGSEIPYWFTTK-MGSYISVKWDPKAPNAHLIHF-A 194
             +HR +     K+   K   +   PGS+IP  F  + MG  ++++      ++ ++ F A
Sbjct: 1069 LIHRNL-----KLESAKPEHS-YFPGSDIPTCFNHQVMGPSLNIQLPQSESSSDILGFSA 1128

Query: 195  CVLCGPIDDQDDMADVSGSIVASFTGKNHEDSNLKNGD---LLV---------NGFTISG 254
            C++ G +D Q  M ++          K H    LK+ D   L+V           FT   
Sbjct: 1129 CIMIG-VDGQYPMNNL----------KIHCSCILKDADACELVVMDEVWYPDPKAFTNMY 1188

Query: 255  MRKLDHIWLFVLPRTRLLTRKINKCKEIEFRFLFQANYGQSVTPNIELKKCGVGLINME 297
                DH+ LF    +R  T  +    E  F F  +   G S +P  E+KKC V LI+++
Sbjct: 1189 FGS-DHLLLF----SRTCT-SMEAYSEALFEFSVENTEGDSFSPLGEVKKCAVHLISLK 1221

[Close]

BLAST of Spg021481 vs. TAIR 10
Match: AT4G12010.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 85.5 bits (210), Expect = 8.5e-17
Identity = 85/339 (25.07%), Postives = 146/339 (43.07%), Query Frame = 0


Query: 3    TDTAPSSSIFGIPSLAGLYSLTRLNLRDCNLEVIPQGIECLLSLTELNLCGNNFSHLPTS 62
            T +  S S+F +P   G   LT L L  C+L  +P  I  L SL  L L GNN  +LP S
Sbjct: 837  TSSHVSVSMFFMPPTLGCSRLTDLYLSRCSLYKLPDNIGGLSSLQSLCLSGNNIENLPES 896

Query: 63   ISQLHNLKRFNIKQCKKLLHFPELPPRILRLFSKDCVSLKSIPD----ISKVEHLYFMTE 122
             +QL+NLK F++K CK L   P LP  +  L + +C SL+++ +    ++  E ++ M  
Sbjct: 897  FNQLNNLKWFDLKFCKMLKSLPVLPQNLQYLDAHECESLETLANPLTPLTVGERIHSM-- 956

Query: 123  LNLMNFYQLTNNKEL---------HRMVVSWMEKMLFR----KGAFNIMIPGSEIPYWFT 182
                N Y+L  + +           +++ +   K  +R    +    I  P +EIP WF 
Sbjct: 957  FIFSNCYKLNQDAQASLVGHARIKSQLMANASAKRYYRGFVPEPLVGICYPATEIPSWFC 1016

Query: 183  -TKMGSYISVKWDPKAPNAHLIHFACVLCGPIDDQDDMAD-VSGSIVASFTGKNHEDSNL 242
              ++G  + +   P   + + +  A  +     D +D A   S     +F  K   DS+ 
Sbjct: 1017 HQRLGRSLEIPLPPHWCDINFVGLALSVVVSFKDYEDSAKRFSVKCCGNFENK---DSSF 1076

Query: 243  KNGDLLVNGFT------ISGMRKLDHIWLFVLPRTRLLTRKI----NKC--KEIEFRFLF 302
               D  + G+           RKL    +F+   +  L + +    N C   +  F F  
Sbjct: 1077 TRFDFTLAGWNEPCGSLSHESRKLTSDHVFMGYNSCFLVKNVHGESNSCCYTKASFEFYV 1136

Query: 303  QANYGQSVTPNIELKKCGVGLINMEKNKEAMKCYASRII 311
              +  +      E+ KCG+ L+ + ++ + M    + I+
Sbjct: 1137 TDDETRKKIETCEVIKCGMSLMYVPEDDDCMLLKKTNIV 1170

[Close]

BLAST of Spg021481 vs. TAIR 10
Match: AT5G45200.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 81.6 bits (200), Expect = 1.2e-15
Identity = 76/296 (25.68%), Postives = 122/296 (41.22%), Query Frame = 0


Query: 30   DCNLEVIPQGIECLLSLTELNLCGNNFSHLPTSISQLHNLKRFNIKQCKKLLHFPELPPR 89
            +C+L   P+GI  L S+  L+L  N F  LP SI  L++L   ++K CK L+  P LPP 
Sbjct: 857  NCHLCEWPRGIYGLSSVRRLSLSSNEFRILPRSIGYLYHLNWLDLKHCKNLVSVPMLPPN 916

Query: 90   ILRLFSKDCVSLKSI-----PDISKVEHLY-FMTELNLMNFYQLTNNK---------ELH 149
            +  L +  C+SL++I     P +++ EHL+      N    Y++  N          +L 
Sbjct: 917  LQWLDAHGCISLETISILSDPLLAETEHLHSTFIFTNCTKLYKVEENSIESYPRKKIQLM 976

Query: 150  RMVVSWMEKMLFRKGAFNIMIPGSEIPYWFTTKMGSYISVKWDPKAPNAHLIHFACVLCG 209
               ++  EK L       I  PG ++P WF  +       +  P+  NA  +     LC 
Sbjct: 977  SNALARYEKGLALDVLIGICFPGWQVPGWFNHRTVGLELKQNLPRHWNAGGL-AGIALCA 1036

Query: 210  PIDDQDDMADVSGSIVASFTGKNHEDSNLKNGDLLVNGFTISGMR-----KLDHIWLFVL 269
             +  +D ++  +  +V        ED  L     ++ G+T  G       K DH+++   
Sbjct: 1037 VVSFKDYISKNNRLLVTCSGEFKKEDKTLFQFSCILGGWTEHGSYEAREIKSDHVFIGYT 1096

Query: 270  PRTRLLTRKIN-KCKEIEFRFLFQANYGQSVTPNIELKKCGVGLINMEKNKEAMKC 305
                 +    +  C   E    FQ   G     N  + KCG  LI    N +   C
Sbjct: 1097 SWLNFMKSDDSIGCVATEASLRFQVTDGTREVTNCTVVKCGFSLIYSHTNVDHSLC 1151

[Close]

BLAST of Spg021481 vs. TAIR 10
Match: AT5G45250.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 72.4 bits (176), Expect = 7.4e-13
Identity = 82/289 (28.37%), Postives = 124/289 (42.91%), Query Frame = 0


Query: 33   LEVIPQGIECLLSLTELNLCGN-NFSHLPTSISQLHNLKRFNIKQCKKLLHFPELPPRIL 92
            +EV+PQ    L S+  L L  N   S LP  ISQL  LK  ++K C  L   PE PP + 
Sbjct: 831  IEVMPQ----LPSVQYLCLSRNAKISCLPVGISQLSQLKWLDLKYCTSLTSVPEFPPNLQ 890

Query: 93   RLFSKDCVSLKSIPDISKVEHLYFMTELNLMNFY-------------QLTNNKELHRMVV 152
             L +  C SLK+   +SK       TE N   F              ++T+  +    ++
Sbjct: 891  CLDAHGCSSLKT---VSKPLARIMPTEQNHSTFIFTNCENLEQAAKEEITSYAQRKCQLL 950

Query: 153  SWMEKM----LFRKGAFNIMIPGSEIPYWFTTK-MGSYISVKWDPKAPNAHLIHFA-CVL 212
            S+  K     L  +  F+   PG E+P WF  + +GS + VK  P   +  L   A C +
Sbjct: 951  SYARKRYNGGLVSESLFSTCFPGCEVPSWFCHETVGSELEVKLLPHWHDKKLAGIALCAV 1010

Query: 213  CGPIDDQDDMADVSGSIVASFTGKNHEDSNLKNGDLLVNGFTISGMRK----LDHIWL-- 272
               +D QD ++ +  S+  +F  K+ ED +       V  +T  G  K    LDH+++  
Sbjct: 1011 VSCLDPQDQVSRL--SVTCTFKVKD-EDKSWVAYTCPVGSWTRHGGGKDKIELDHVFIGY 1070

Query: 273  FVLPRTRLLTRKIN--KCKEIEFRFLFQANYGQSVTPNIELKKCGVGLI 294
               P T     + N  +C   E    F    G S     ++ KCG+ L+
Sbjct: 1071 TSCPHTIKCHEEGNSDECNPTEASLKFTVTGGTSENGKYKVLKCGLSLV 1109

[Close]

BLAST of Spg021481 vs. TAIR 10
Match: AT1G57650.1 (ATP binding )

HSP 1 Score: 68.9 bits (167), Expect = 8.2e-12
Identity = 60/219 (27.40%), Postives = 105/219 (47.95%), Query Frame = 0


Query: 4   DTAPSSSIFGIPSLAGLYSLTRLNLRDC-NLEVIPQGIECLLSLTELNL--CGNNFSHLP 63
           D   S ++  +P+L+   +L  LNL  C +L  +P  I    SL +L L  C ++   LP
Sbjct: 366 DLTDSRNLKELPNLSMATNLKNLNLERCSSLVELPSSIGNATSLHDLRLFKC-SSLVELP 425

Query: 64  TSISQLHNLKRFNIKQCKKLLHFPELPPRILRLFSKDCVSLKSIPDISKVEHLYFMTELN 123
            SI  L NL + ++++C  L+  P+LP  I+ L +++C SL+ + D S       +  +N
Sbjct: 426 FSIGNLTNLWKLDLRECSSLVSLPQLPDSIMVLNARNCESLEKL-DCSFYNPGILLNFVN 485

Query: 124 LMNFYQLTNNKELHRMVVSWMEKMLFRKGAFNIMIPGSEIPYWFTTKM-GSYISVKWDPK 183
             N  Q   +  +    V++            +++PG E+P  FT +  GS +SVK + K
Sbjct: 486 CFNLNQEARDLLIETSTVNF------------VVLPGKEVPACFTYRSHGSSVSVKVNQK 545

Query: 184 APNAHLIHFACVLC-GPIDDQDDMADVSGSIVASFTGKN 218
             +      AC+L    +D++    D+    V + T K+
Sbjct: 546 LLHTSTKFKACILFENEVDNETYYFDLDTLCVYTKTNKD 570

The following BLAST results are available for this feature:

BLAST of Spg021481 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6592347.1	7.0e-122	69.33	Disease resistance protein RUN1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023535476.1	2.0e-121	68.69	TMV resistance protein N-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022925367.1	5.9e-121	69.01	TMV resistance protein N-like [Cucurbita moschata]	[more]
XP_022973476.1	7.7e-121	68.69	TMV resistance protein N-like [Cucurbita maxima]	[more]
XP_022141876.1	8.5e-120	70.38	TMV resistance protein N-like isoform X1 [Momordica charantia]	[more]

BLAST of Spg021481 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SZ66	1.2e-15	25.07	Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=...	[more]
Q9SCX7	1.0e-11	28.37	Inactive disease resistance protein RPS4 OS=Arabidopsis thaliana OX=3702 GN=RPS4...	[more]
C4B7M7	1.0e-11	28.37	Disease resistance protein RPS4 OS=Arabidopsis thaliana OX=3702 GN=RPS4 PE=2 SV=...	[more]
Q9XGM3	1.0e-11	28.37	Disease resistance protein RPS4 OS=Arabidopsis thaliana OX=3702 GN=RPS4 PE=1 SV=...	[more]
Q40392	1.0e-11	37.90	TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1	[more]

BLAST of Spg021481 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EBI7	2.9e-121	69.01	TMV resistance protein N-like OS=Cucurbita moschata OX=3662 GN=LOC111432675 PE=4...	[more]
A0A6J1I8R5	3.7e-121	68.69	TMV resistance protein N-like OS=Cucurbita maxima OX=3661 GN=LOC111472025 PE=4 S...	[more]
A0A6J1CKI7	4.1e-120	70.38	TMV resistance protein N-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
M4R4K0	1.8e-115	67.63	Resistance gene-like protein OS=Cucumis melo OX=3656 GN=RGH14 PE=4 SV=1	[more]
A0A1S3CBN9	2.3e-115	67.63	TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498654 PE=4 SV=1	[more]

BLAST of Spg021481 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17680.1	2.9e-17	30.10	disease resistance protein (TIR-NBS-LRR class), putative	[more]
AT4G12010.1	8.5e-17	25.07	Disease resistance protein (TIR-NBS-LRR class) family	[more]
AT5G45200.1	1.2e-15	25.68	Disease resistance protein (TIR-NBS-LRR class) family	[more]
AT5G45250.1	7.4e-13	28.37	Disease resistance protein (TIR-NBS-LRR class) family	[more]
AT1G57650.1	8.2e-12	27.40	ATP binding	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003591	Leucine-rich repeat, typical subtype	SMART	SM00369	LRR_typ_2	coord: 43..66 e-value: 0.44 score: 19.6 coord: 20..42 e-value: 12.0 score: 12.5
IPR001611	Leucine-rich repeat	PFAM	PF13855	LRR_8	coord: 22..77 e-value: 9.0E-7 score: 28.6
IPR001611	Leucine-rich repeat	PROSITE	PS51450	LRR	coord: 45..66 score: 7.142103
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 5..178 e-value: 1.1E-18 score: 68.8
None	No IPR available	PANTHER	PTHR11017:SF375	DISEASE RESISTANCE PROTEIN TAO1-LIKE	coord: 31..246
None	No IPR available	SUPERFAMILY	52058	L domain-like	coord: 10..128
IPR044974	Disease resistance protein, plants	PANTHER	PTHR11017	LEUCINE-RICH REPEAT-CONTAINING PROTEIN	coord: 31..246

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg021481.1	Spg021481.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0015074	DNA integration
biological_process	GO:0007165	signal transduction
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0003953	NAD+ nucleosidase activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0005515	protein binding