Spg021475 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg021475
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionEndoglucanase
Locationscaffold9: 7012614 .. 7017805 (+)
RNA-Seq ExpressionSpg021475
SyntenySpg021475
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTATTTTCTTAAACTCTTCTTCCCATGGCTGCCATCACAAACTCTTCAACTTTCTTCTTCTTCTTCTTGCTCTTGCTTCCATTCTCATTGATAGGTAGCGCCTGGGGCGATCCGAATTATAGAGACGCCTTGGCTAAGTCGATATTGTTCTTCGAAGGACAACGCTCCGGTAGGATCCCGGCTGGCCAGCAGATTACTTGGAGGTCCAACTCCGGTCTCTACGATGGTGAACTTGCTCATGTATGTGTTAACGCATTTCTTTATTCCTTAGAAAAATTGTTTCTTGTTTCTTGTCACTTAAATTAAAATAGAACTACATTTGATAAGTGATTCATAACTCTTGTGTTTTTTTAATCAATACGAAATTAACACTACATTAGACCATGTTTTATTTTTAATCTCTCAAAATTTGAATTACAAAATAACATTTTTTTATGGAAAAGTAAATCTTTAAATAAAATAACCAACATGATTTTTTTTTTCACATCAAATATGATATGGTCATGTATATATCTTCCACTCAAATATTAGAATTTTAAATTTCCTAACTGTATTGTTGAACTAAAAAAGAAAAAGAGAACCGTGAAGAGAGGTGACATCAACCAACCAACATCATTATATATATTTTTTTTAAATACCAACATCATTTTTTTTTTTTTGAAAAATATGAAGTGTGAGATTTGAACCATAAACCTCGTGGTTATCAGTACAACTTTATGCCTAGTTGACTTGTTAGCGAACAACATTATATAGTAAAACTAATAAAATAATAAATTTATTAAATATTTCTATAAAAACTAGATTTTGTTGTTAAAAAAAGAACTGTGAATTATCACAGTTTTTTTTCCCCTTACAAATTAGAAAGAAAAAAAAAGAAGGAACTTTACCAAAATCATGGGTAAAAATTTCAGTTTAGAATTATGTTAAGAGTTGGAGAAGTGAAACCCAATGGTTTGGTAATTCTCAGGTGGATTTAACCGGCGGGTACTACGATGCCGGCGACAATGTGAAATTCAACTTTCCGATGGCTTTCACAACGACAATGCTTTCATGGGGAGCACTCGAGTACGGGGCGCGTATGGGCACGCAGTTAGCAAACACACGCGCCGCCATCCGTTGGGCCACCGATTACCTTCTCAAGTGCGCCACCGCCACTCCCGGCAAGCTCTACGTCGGCGTCGGCGACCCCAACGTCGACCACAAGTGCTGGGAGCGCCCCGAGGACATGGATACGGTTCGGACTGTTTACTCTGTCTCCGCCGGAAACCCTGGATCCGACGTTGCCGGCGAGACAGCCGCCGCCCTCGCCGCCGCGTCGTTGGTGTTCCGGCGGGTTGATCGTAAGTACTCCGGGCTGCTACTGGCGACGGCGAAGAAGGTGCTGCAGTTTGCCGTTGAGCATCGAGGGTCGTATAGTGATTCGCTTGGATCTGCTGTTTGTCCCTTTTATTGCTCTTATTCTGGATATAAGGTGAGGAATTATTTTTTCCTGTCTTTTGTTTTTTAAGATTAAAACTTTAGGAAAATTTTCTAAATATTTAGAAATTTATTTTCTTACATTATTTTCTTGAAAAATATTTGTGGATGGCGGCTTAAAAGTTGTAAATGGACCCACTTATCCATTAGAAAATTATATATATATTTATCCTCACTAAATATTTATTATTTTTTTTTTTAAAAAAAAAACTTCTTTTCTATATATATTGTCTTCATATATATGGTGGTTGGATTTTTGTCTTTAAAATACGAATAGATTTATTTAATGAATTATATTTTTTGTGTTTCAAATATTATAAATTAAAATAAATATTTAAAATTTTAAATTTAATCGGTTATTTCTATTTTTGTCCAAATTTAACTTTGTAGTATGATTATTTTGAAAAAGAAAAAGATTTTGTTGTTTTGGCAAGGATATTGTTGAGATTTTGAAGATTTTGAAATTTAAAGTGGCGATCGTTTGTAGATAGATAAGGAAATAAACATTAATTCGGCTTCATTTGGAAATTTTTTGTGTTTTTGTTTTTTTTTTAAAAAAAAAAATTTAAGACAAATTAATGTAGGAACTATAGTTTGTTTAGTTTTTAAAATTTAGGAAACATTTTTAAAATCGTCTTTTTCATAGATTTTATATGACAAAGAAAACGGGTTTTAAAATCAAGTTTTTTTTTTTAAAAAAAAAATCATTTATTGAGTTCAAAGAATCAAGTCTTAATACCTTGTCTAATAATTTCTGATTTGATATTAGTTAGAAAAGGGTAAATTGGGAAATACCTTCATGGAAGGAAAGTTTTGTTAATTTATTATTGAGTTTTTGGAATGGGGATTTAATAATGATTTTTTGTTTTTGATAATATATGGGGTGGAGGATTCGAACCACAGACTATTATCATTAGTACAATATTATGCTAGTTGAGCTATGTTCTTTGTTGAGGAACCCACATTGAAAAGATTAGTGGAGACCTCACAATTTATAAACTACAAGGGTCACCTCACTCATGGCCAATTAGTTTTGAGATGAAAGTCCATGTTATTTGATATGGTATCAAAGTCCATAAAACCCAAATGGGTATTCAGTCAAAGAAAAAGAGGCACCATCTTGAACCAATTGATTTTGAGATGGAAGCCTATGTTATTTGATATTATTGTTGACTAATAATGACATATTTATGATATGAAAATTTTGTTGATGAATACAGGATGAGTTGGTGTGGGGTGCAACATGGCTTCTAAGAGCAACAAATGATGTTCAATATTTCAATTTGTTGAAGTCATTGGGAGGTGATGATGTGACTGACATCTTCAGTTGGGACAACAAATATGCTGGTGCTCATGTTCTCTTGTCTAGGGTAAATAATATTTAATTTCCCTCTATTTTCGCTCTATGCAATTTATTATTATTATTATTATAAAAAGTTAAAATATCACTTTTGAAACTCATTTTATTTTAGTTTTAGTATTATCAAATATTCAAATTTAGTTTTTTACTCTCAATAAATCTTAAAATAATTTGGAGTTAATTTTTATTGAATTGATAAAATAATAATAATAATTTTTACACCAATTAAAAAGAAACAATGTCAATATCTTTCCAAATTTTATAGAGGAAAGGTTAATAGTGACTTTTTTTTTTTTTACTTAACAATAAACTAATCATAAGAACTTTTATCGAAAAGTACAAGGATTGAATTTGACTAAATTTGAAATATTTGAAAATTCGAGGATTAAACTAACATCAGTTCCAAAGACAAAAATGATAATATATAGATACACACACAAAATTACTTTTAACCATAATACCAATTTAAATGGTTAAATATTAATTTCCCTCCTTTATTTTTTTTTTTTTTTTACCTAGATTATAGAATTTGAAACCTCTCCTCTTCTCATTATTTGGTAGGTAATATATTTTAATTTTTCATTTAAAATGGTTGGTTTTTGTTCGTGCAGCGAGCATTATTAAACAATGACAAGAACTTCGACTCCTACAAACAAGAAGCCGAGGCATTCATGTGTCGTATTCTACCAAAATCTCCATATTCTAGCACTCAATACACACAAGGTATGCAACTTACCAGACAAACAACTTAAACATAGTTCAATTGTTTGGGCATGTAACGTACAATGAATAAGTGGTACATTTGTGGGCTTTAGTTCATTTCTTTGGTTTGTAATTCATGAATCTTAATTTGAGAAAAATAATAAAAATAAATTAACAGCTAATTGAGTATATGTTAAATTGAATTTGATTTTGTGTAGGGGGATTGATGTTCAAGTTGCCAGAAAGTAACCTCCAATATGTGACATCCATAACGTTTTTGCTGACCACATATTCCAAGTACATGTCCGCCGCCAAGCACACCTTCAACTGCGGCAGCCTTCTTGTCACTCCGGCTTCCCTCAAGAACCTTGCTAAGGAACAGGTAACTACCGAAACGCGGTCATTTTAGATCACTTCAAAGTTCAAACTACTTTTTGAGATCCTATTTGATAATCATTTGGTTTTTGAAAATTCCAAGATAATGCAAAACATAATAAAAAAAAGTACTCAAGTTCAAGATAATGTAAAACATAATCACTCTTGTTATATTTAAAAAAAATGAAAAGTTGGGATATTTTTTTTTAGAATGTATATTAAATTAATATACTACTATACCAAGGGGACATTCTCTAGAGGCAAATGAACCCATGTCCAAAAAACAAATAATGAAGTTTAAACATTTAGGCCCCGTTTGATAACCATTTGGTTTTTAAGTTTATAAACACCACTTCCAACTCTAATTTTCTTTGACTTGTCACCTACCTTCCCATGTTTTAAAAAACCAAGTCAATTTTTGAAAACTAAAAAAAGTAGTTTTCAAAAACTTGTTTTTGTTTTTAGAATTTGGTTAGAAATTCAAGTGTGTCTTTAACAAAAATGAAAACCATGGTGAAGAAATTTAAAGAAAACAAGTCTAATTTTCAAAAACCAAAAATAAAAAACCAAATGGTTATCAAACGGGGCCTTAATAAAACTAAGGTTTTGTTTAGTTTTTTTAGATTTAATCCAATTGTAAATACTAAATCAAGATAAATTTTAAAACTAAATTGAAGTCCACACAAATTATAAGGACTAAAATGTTAATTTTATTTTTTAAAAAAATCTTGATGTGGAGTAAGAATTCAATTTTTTTTTTTTTCTTGACAATATCTGGAGAGGGAGATTCGAATCACATACCTTGTTGTTATTAGTACAACTTTATGCCAATTGAGCTAAGCTCTTGTTGGCAAGAATTCAAATATTTTAATTTGGAAAACTTAAAATTATTTTTGTATATGTATAAATATAAATTTAAAGATTTACATGTTTTTCTTTTGAATTATAAAATTCAGGTGGATTATATATTGGGAGTGAACCCATTAAAAATGTCATACATGGTGGGATTTGGAAAAAGCTTCCCACGAAGAATTCACCACAGAGGATCTTCGCTGCCGTCGAAGGCGAGCCATCCTCAGGCCATTGGTTGCGACGGCGGCTTCCAGCCCTTCTTCTACTCCTACAACCCCAACCCCAACATCTTAACCGGCGCCGTCGTCGGCGAGCCATCCTCAGGCCATTGGTTGCGACGGCGGCTTCCAGCCCTTCTTCTACTCCTACAACCCCAACCCCAACATCTTAACCGGCGCCGTCGTCGGCGGTCCGAACCAGAGTGA

mRNA sequence

ATGGCTGCCATCACAAACTCTTCAACTTTCTTCTTCTTCTTCTTGCTCTTGCTTCCATTCTCATTGATAGGTAGCGCCTGGGGCGATCCGAATTATAGAGACGCCTTGGCTAAGTCGATATTGTTCTTCGAAGGACAACGCTCCGGTAGGATCCCGGCTGGCCAGCAGATTACTTGGAGGTCCAACTCCGGTCTCTACGATGGTGAACTTGCTCATGTGGATTTAACCGGCGGGTACTACGATGCCGGCGACAATGTGAAATTCAACTTTCCGATGGCTTTCACAACGACAATGCTTTCATGGGGAGCACTCGAGTACGGGGCGCGTATGGGCACGCAGTTAGCAAACACACGCGCCGCCATCCGTTGGGCCACCGATTACCTTCTCAAGTGCGCCACCGCCACTCCCGGCAAGCTCTACGTCGGCGTCGGCGACCCCAACGTCGACCACAAGTGCTGGGAGCGCCCCGAGGACATGGATACGGTTCGGACTGTTTACTCTGTCTCCGCCGGAAACCCTGGATCCGACGTTGCCGGCGAGACAGCCGCCGCCCTCGCCGCCGCGTCGTTGGTGTTCCGGCGGGTTGATCGTAAGTACTCCGGGCTGCTACTGGCGACGGCGAAGAAGGTGCTGCAGTTTGCCGTTGAGCATCGAGGGTCGTATAGTGATTCGCTTGGATCTGCTGTTTGTCCCTTTTATTGCTCTTATTCTGGATATAAGGATGAGTTGGTGTGGGGTGCAACATGGCTTCTAAGAGCAACAAATGATGTTCAATATTTCAATTTGTTGAAGTCATTGGGAGGTGATGATGTGACTGACATCTTCAGTTGGGACAACAAATATGCTGGTGCTCATGTTCTCTTGTCTAGGCGAGCATTATTAAACAATGACAAGAACTTCGACTCCTACAAACAAGAAGCCGAGGCATTCATGTGTCGTATTCTACCAAAATCTCCATATTCTAGCACTCAATACACACAAGGGGGATTGATGTTCAAGTTGCCAGAAAGTAACCTCCAATATGTGACATCCATAACGTTTTTGCTGACCACATATTCCAAGTACATGTCCGCCGCCAAGCACACCTTCAACTGCGGCAGCCTTCTTGTCACTCCGGCTTCCCTCAAGAACCTTGCTAAGGAACAGGTGGATTATATATTGGGAGTGAACCCATTAAAAATGTCATACATGGTGGGATTTGGAAAAAGCTTCCCACGAAGAATTCACCACAGAGGATCTTCGCTGCCGTCGAAGGCGAGCCATCCTCAGGCCATTGGTTGCGACGGCGGCTTCCAGCCCTTCTTCTACTCCTACAACCCCAACCCCAACATCTTAACCGGCGCCGTCGTCGGCGAGCCATCCTCAGGCCATTGGTTGCGACGGCGGCTTCCAGCCCTTCTTCTACTCCTACAACCCCAACCCCAACATCTTAACCGGCGCCGTCGTCGGCGGTCCGAACCAGAGTGA

Coding sequence (CDS)

ATGGCTGCCATCACAAACTCTTCAACTTTCTTCTTCTTCTTCTTGCTCTTGCTTCCATTCTCATTGATAGGTAGCGCCTGGGGCGATCCGAATTATAGAGACGCCTTGGCTAAGTCGATATTGTTCTTCGAAGGACAACGCTCCGGTAGGATCCCGGCTGGCCAGCAGATTACTTGGAGGTCCAACTCCGGTCTCTACGATGGTGAACTTGCTCATGTGGATTTAACCGGCGGGTACTACGATGCCGGCGACAATGTGAAATTCAACTTTCCGATGGCTTTCACAACGACAATGCTTTCATGGGGAGCACTCGAGTACGGGGCGCGTATGGGCACGCAGTTAGCAAACACACGCGCCGCCATCCGTTGGGCCACCGATTACCTTCTCAAGTGCGCCACCGCCACTCCCGGCAAGCTCTACGTCGGCGTCGGCGACCCCAACGTCGACCACAAGTGCTGGGAGCGCCCCGAGGACATGGATACGGTTCGGACTGTTTACTCTGTCTCCGCCGGAAACCCTGGATCCGACGTTGCCGGCGAGACAGCCGCCGCCCTCGCCGCCGCGTCGTTGGTGTTCCGGCGGGTTGATCGTAAGTACTCCGGGCTGCTACTGGCGACGGCGAAGAAGGTGCTGCAGTTTGCCGTTGAGCATCGAGGGTCGTATAGTGATTCGCTTGGATCTGCTGTTTGTCCCTTTTATTGCTCTTATTCTGGATATAAGGATGAGTTGGTGTGGGGTGCAACATGGCTTCTAAGAGCAACAAATGATGTTCAATATTTCAATTTGTTGAAGTCATTGGGAGGTGATGATGTGACTGACATCTTCAGTTGGGACAACAAATATGCTGGTGCTCATGTTCTCTTGTCTAGGCGAGCATTATTAAACAATGACAAGAACTTCGACTCCTACAAACAAGAAGCCGAGGCATTCATGTGTCGTATTCTACCAAAATCTCCATATTCTAGCACTCAATACACACAAGGGGGATTGATGTTCAAGTTGCCAGAAAGTAACCTCCAATATGTGACATCCATAACGTTTTTGCTGACCACATATTCCAAGTACATGTCCGCCGCCAAGCACACCTTCAACTGCGGCAGCCTTCTTGTCACTCCGGCTTCCCTCAAGAACCTTGCTAAGGAACAGGTGGATTATATATTGGGAGTGAACCCATTAAAAATGTCATACATGGTGGGATTTGGAAAAAGCTTCCCACGAAGAATTCACCACAGAGGATCTTCGCTGCCGTCGAAGGCGAGCCATCCTCAGGCCATTGGTTGCGACGGCGGCTTCCAGCCCTTCTTCTACTCCTACAACCCCAACCCCAACATCTTAACCGGCGCCGTCGTCGGCGAGCCATCCTCAGGCCATTGGTTGCGACGGCGGCTTCCAGCCCTTCTTCTACTCCTACAACCCCAACCCCAACATCTTAACCGGCGCCGTCGTCGGCGGTCCGAACCAGAGTGA

Protein sequence

MAAITNSSTFFFFFLLLLPFSLIGSAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITWRSNSGLYDGELAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEYGARMGTQLANTRAAIRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAGETAAALAAASLVFRRVDRKYSGLLLATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGYKDELVWGATWLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNFDSYKQEAEAFMCRILPKSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAKHTFNCGSLLVTPASLKNLAKEQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKASHPQAIGCDGGFQPFFYSYNPNPNILTGAVVGEPSSGHWLRRRLPALLLLLQPQPQHLNRRRRRRSEPE
Homology
BLAST of Spg021475 vs. NCBI nr
Match: XP_038890642.1 (endoglucanase 9-like [Benincasa hispida])

HSP 1 Score: 877.1 bits (2265), Expect = 7.1e-251
Identity = 425/453 (93.82%), Postives = 437/453 (96.47%), Query Frame = 0

Query: 2   AAITNSSTFFFFFLLLLPFSLIGSAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITWRS 61
           AAITNSST FFFFLLLL FSL+    GDPNYRDAL+KSILFFEGQRSGRIPA Q+ITWRS
Sbjct: 3   AAITNSSTLFFFFLLLLSFSLVDHTRGDPNYRDALSKSILFFEGQRSGRIPANQRITWRS 62

Query: 62  NSGLYDGELAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEYGARMGTQLANTRAAI 121
           NSGLYDGELAHVDLTGGYYDAGDNVKFN PMAFTTTMLSWGALEYGARMGT+L N RAAI
Sbjct: 63  NSGLYDGELAHVDLTGGYYDAGDNVKFNLPMAFTTTMLSWGALEYGARMGTELPNARAAI 122

Query: 122 RWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAGET 181
           RWATDYLLKCATATPGKLYVGVGDP+VDHKCWERPEDMDTVRTVYSVSAGNPGSDVA ET
Sbjct: 123 RWATDYLLKCATATPGKLYVGVGDPHVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAAET 182

Query: 182 AAALAAASLVFRRVDRKYSGLLLATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGYKD 241
           AAALAAASLVFRRVDRKYS +LLATAKKV+QFA+EHRGSYSDSL SAVCPFYCSYSGYKD
Sbjct: 183 AAALAAASLVFRRVDRKYSRVLLATAKKVMQFALEHRGSYSDSLSSAVCPFYCSYSGYKD 242

Query: 242 ELVWGATWLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNFD 301
           ELVWGATWLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNFD
Sbjct: 243 ELVWGATWLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNFD 302

Query: 302 SYKQEAEAFMCRILPKSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAKH 361
           SYKQEAE+FMCRILP SPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAKH
Sbjct: 303 SYKQEAESFMCRILPNSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAKH 362

Query: 362 TFNCGSLLVTPASLKNLAKEQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKASH 421
           TFNCGSL+VTPASLKNLAK+QVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKASH
Sbjct: 363 TFNCGSLIVTPASLKNLAKKQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKASH 422

Query: 422 PQAIGCDGGFQPFFYSYNPNPNILTGAVVGEPS 455
           PQAI CDGGFQPFFYSYNPNPNILTGAVVG P+
Sbjct: 423 PQAIACDGGFQPFFYSYNPNPNILTGAVVGGPN 455

BLAST of Spg021475 vs. NCBI nr
Match: XP_023523831.1 (endoglucanase 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 859.8 bits (2220), Expect = 1.2e-245
Identity = 416/454 (91.63%), Postives = 431/454 (94.93%), Query Frame = 0

Query: 1   MAAITNSSTFFFFFLLLLPFSLIGSAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITWR 60
           MAA TN+ TFFFF LLL       +A  +PNYRDALAKS+LFF+GQRSGRIP GQQI WR
Sbjct: 1   MAATTNAPTFFFFLLLLSSSFSFYTARANPNYRDALAKSLLFFQGQRSGRIPNGQQIAWR 60

Query: 61  SNSGLYDGELAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEYGARMGTQLANTRAA 120
           SNSGLYDGELAHVDLTGGYYDAGDNVKFN PMAFTTTMLSWGALEYGARMG++L NTRAA
Sbjct: 61  SNSGLYDGELAHVDLTGGYYDAGDNVKFNLPMAFTTTMLSWGALEYGARMGSELPNTRAA 120

Query: 121 IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAGE 180
           IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSA NPGSDVAGE
Sbjct: 121 IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAANPGSDVAGE 180

Query: 181 TAAALAAASLVFRRVDRKYSGLLLATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGYK 240
           TAAALAAASLVFRRVDRKYSGLLLATAKKV QFAVEHRGSYSDSLGSAVCPFYCSYSGYK
Sbjct: 181 TAAALAAASLVFRRVDRKYSGLLLATAKKVFQFAVEHRGSYSDSLGSAVCPFYCSYSGYK 240

Query: 241 DELVWGATWLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNF 300
           DELVWGATWLLRATNDV+YFNLLKSLGGDDV DIFSWDNKYAGAHVLLSRRALLNNDKNF
Sbjct: 241 DELVWGATWLLRATNDVRYFNLLKSLGGDDVPDIFSWDNKYAGAHVLLSRRALLNNDKNF 300

Query: 301 DSYKQEAEAFMCRILPKSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAK 360
           DSYKQEAE+FMCRILP SPYSSTQYTQGGLMFKLP+SNLQYVTSITFLLTTYSKYMSAAK
Sbjct: 301 DSYKQEAESFMCRILPNSPYSSTQYTQGGLMFKLPQSNLQYVTSITFLLTTYSKYMSAAK 360

Query: 361 HTFNCGSLLVTPASLKNLAKEQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKAS 420
           HTFNCG +LVTP SLKNLAK+QVDYILGVNPLKMSYMVGFGK+FP+RIHHRGSSLPSKAS
Sbjct: 361 HTFNCGGVLVTPTSLKNLAKQQVDYILGVNPLKMSYMVGFGKTFPKRIHHRGSSLPSKAS 420

Query: 421 HPQAIGCDGGFQPFFYSYNPNPNILTGAVVGEPS 455
           HPQAIGCDGGFQPFFYSYNPNPNILTGAVVG P+
Sbjct: 421 HPQAIGCDGGFQPFFYSYNPNPNILTGAVVGGPN 454

BLAST of Spg021475 vs. NCBI nr
Match: KAG6607530.1 (Endoglucanase 9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 858.2 bits (2216), Expect = 3.4e-245
Identity = 415/454 (91.41%), Postives = 431/454 (94.93%), Query Frame = 0

Query: 1   MAAITNSSTFFFFFLLLLPFSLIGSAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITWR 60
           MAA TN+ TFFFF LLL       +A  +PNYRDALAKS+LFF+GQRSGRIP GQQI WR
Sbjct: 1   MAATTNAPTFFFFILLLSSSFSFYTARANPNYRDALAKSLLFFQGQRSGRIPNGQQIAWR 60

Query: 61  SNSGLYDGELAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEYGARMGTQLANTRAA 120
           SNSGLYDGELAHVDLTGGYYDAGDNVKFN PMAFTTTMLSWGALEYGARMG++L NTRAA
Sbjct: 61  SNSGLYDGELAHVDLTGGYYDAGDNVKFNLPMAFTTTMLSWGALEYGARMGSELPNTRAA 120

Query: 121 IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAGE 180
           IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSA NPGSDVAGE
Sbjct: 121 IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAANPGSDVAGE 180

Query: 181 TAAALAAASLVFRRVDRKYSGLLLATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGYK 240
           TAAALAAASLVFRRVDRKYSGLLLATAKKV QFAVEHRGSYSDSLGSAVCPFYCSYSGYK
Sbjct: 181 TAAALAAASLVFRRVDRKYSGLLLATAKKVFQFAVEHRGSYSDSLGSAVCPFYCSYSGYK 240

Query: 241 DELVWGATWLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNF 300
           DELVWGATWLLRATNDV+YFNLLKSLGGDDV DIFSWDNKYAGAHVLLSRRALLNNDKNF
Sbjct: 241 DELVWGATWLLRATNDVRYFNLLKSLGGDDVPDIFSWDNKYAGAHVLLSRRALLNNDKNF 300

Query: 301 DSYKQEAEAFMCRILPKSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAK 360
           DSYKQ+AE+FMCRILP SPYSSTQYTQGGLMFKLP+SNLQYVTSITFLLTTYSKYMSAAK
Sbjct: 301 DSYKQKAESFMCRILPNSPYSSTQYTQGGLMFKLPQSNLQYVTSITFLLTTYSKYMSAAK 360

Query: 361 HTFNCGSLLVTPASLKNLAKEQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKAS 420
           HTFNCG +LVTP SLKNLAK+QVDYILGVNPLKMSYMVGFGK+FP+RIHHRGSSLPSKAS
Sbjct: 361 HTFNCGGVLVTPTSLKNLAKQQVDYILGVNPLKMSYMVGFGKNFPKRIHHRGSSLPSKAS 420

Query: 421 HPQAIGCDGGFQPFFYSYNPNPNILTGAVVGEPS 455
           HPQAIGCDGGFQPFFYSYNPNPNILTGAVVG P+
Sbjct: 421 HPQAIGCDGGFQPFFYSYNPNPNILTGAVVGGPN 454

BLAST of Spg021475 vs. NCBI nr
Match: XP_022932503.1 (endoglucanase 9-like [Cucurbita moschata])

HSP 1 Score: 858.2 bits (2216), Expect = 3.4e-245
Identity = 416/454 (91.63%), Postives = 432/454 (95.15%), Query Frame = 0

Query: 1   MAAITNSSTFFFFFLLLLPFSLIGSAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITWR 60
           MAA TN+ TFFFF LL   FS   +A  +PNYRDALAKS+LFF+GQRSGRIP GQQI WR
Sbjct: 1   MAATTNAPTFFFFLLLSSSFSFY-TARANPNYRDALAKSLLFFQGQRSGRIPNGQQIAWR 60

Query: 61  SNSGLYDGELAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEYGARMGTQLANTRAA 120
           SNSGLYDGELAHVDLTGGYYDAGDNVKFN PMAFTTTMLSWGALEYGARMG++L NTRAA
Sbjct: 61  SNSGLYDGELAHVDLTGGYYDAGDNVKFNLPMAFTTTMLSWGALEYGARMGSELPNTRAA 120

Query: 121 IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAGE 180
           IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSA NPGSDVAGE
Sbjct: 121 IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAANPGSDVAGE 180

Query: 181 TAAALAAASLVFRRVDRKYSGLLLATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGYK 240
           TAAALAAASLVFRRVDRKYSGLLLATAKKV QFAVEHRGSYSDSLGSAVCPFYCSYSGYK
Sbjct: 181 TAAALAAASLVFRRVDRKYSGLLLATAKKVFQFAVEHRGSYSDSLGSAVCPFYCSYSGYK 240

Query: 241 DELVWGATWLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNF 300
           DELVWGATWLLRATNDV+YFNLLKSLGGDDV DIFSWDNKYAGAHVLLSRRALLNNDKNF
Sbjct: 241 DELVWGATWLLRATNDVRYFNLLKSLGGDDVPDIFSWDNKYAGAHVLLSRRALLNNDKNF 300

Query: 301 DSYKQEAEAFMCRILPKSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAK 360
           DSYKQ+AE+FMCRILP SPYSSTQYTQGGLMFKLP+SNLQYVTSITFLLTTYSKYMSAAK
Sbjct: 301 DSYKQKAESFMCRILPNSPYSSTQYTQGGLMFKLPQSNLQYVTSITFLLTTYSKYMSAAK 360

Query: 361 HTFNCGSLLVTPASLKNLAKEQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKAS 420
           HTFNCG +LVTP SLKNLAK+QVDYILGVNPLKMSYMVGFGK+FP+RIHHRGSSLPSKAS
Sbjct: 361 HTFNCGGVLVTPTSLKNLAKQQVDYILGVNPLKMSYMVGFGKNFPKRIHHRGSSLPSKAS 420

Query: 421 HPQAIGCDGGFQPFFYSYNPNPNILTGAVVGEPS 455
           HPQAIGCDGGFQPFFYSYNPNPNILTGAVVG P+
Sbjct: 421 HPQAIGCDGGFQPFFYSYNPNPNILTGAVVGGPN 453

BLAST of Spg021475 vs. NCBI nr
Match: KAG7037172.1 (Endoglucanase 9, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 857.8 bits (2215), Expect = 4.5e-245
Identity = 417/455 (91.65%), Postives = 432/455 (94.95%), Query Frame = 0

Query: 1   MAAITNSSTFFFFFLLLLPFSL-IGSAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITW 60
           MAA TN  TFFFF LLLL  S    +A  +PNYRDALAKS+LFF+GQRSGRIP GQQI W
Sbjct: 1   MAATTNVPTFFFFLLLLLSSSFSFYTARANPNYRDALAKSLLFFQGQRSGRIPNGQQIAW 60

Query: 61  RSNSGLYDGELAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEYGARMGTQLANTRA 120
           RSNSGLYDGELAHVDLTGGYYDAGDNVKFN PMAFTTTMLSWGALEYGARMG++L NTRA
Sbjct: 61  RSNSGLYDGELAHVDLTGGYYDAGDNVKFNLPMAFTTTMLSWGALEYGARMGSELPNTRA 120

Query: 121 AIRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAG 180
           AIRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSA NPGSDVAG
Sbjct: 121 AIRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAANPGSDVAG 180

Query: 181 ETAAALAAASLVFRRVDRKYSGLLLATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGY 240
           ETAAALAAASLVFRRVDRKYSGLLLATAKKV QFAVEHRGSYSDSLGSAVCPFYCSYSGY
Sbjct: 181 ETAAALAAASLVFRRVDRKYSGLLLATAKKVFQFAVEHRGSYSDSLGSAVCPFYCSYSGY 240

Query: 241 KDELVWGATWLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKN 300
           KDELVWGATWLLRATNDV+YFNLLKSLGGDDV DIFSWDNKYAGAHVLLSRRALLNNDKN
Sbjct: 241 KDELVWGATWLLRATNDVRYFNLLKSLGGDDVPDIFSWDNKYAGAHVLLSRRALLNNDKN 300

Query: 301 FDSYKQEAEAFMCRILPKSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAA 360
           FDSYKQ+AE+FMCRILP SPYSSTQYTQGGLMFKLP+SNLQYVTSITFLLTTYSKYMSAA
Sbjct: 301 FDSYKQKAESFMCRILPNSPYSSTQYTQGGLMFKLPQSNLQYVTSITFLLTTYSKYMSAA 360

Query: 361 KHTFNCGSLLVTPASLKNLAKEQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKA 420
           KHTFNCG +LVTP SLKNLAK+QVDYILGVNPLKMSYMVGFGK+FP+RIHHRGSSLPSKA
Sbjct: 361 KHTFNCGGVLVTPTSLKNLAKQQVDYILGVNPLKMSYMVGFGKNFPKRIHHRGSSLPSKA 420

Query: 421 SHPQAIGCDGGFQPFFYSYNPNPNILTGAVVGEPS 455
           SHPQAIGCDGGFQPFFYSYNPNPNILTGAVVG P+
Sbjct: 421 SHPQAIGCDGGFQPFFYSYNPNPNILTGAVVGGPN 455

BLAST of Spg021475 vs. ExPASy Swiss-Prot
Match: Q9C9H5 (Endoglucanase 9 OS=Arabidopsis thaliana OX=3702 GN=CEL3 PE=1 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 3.1e-201
Identity = 335/446 (75.11%), Postives = 382/446 (85.65%), Query Frame = 0

Query: 9   TFFFFFLLLLPFSLIGSAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITWRSNSGLYDG 68
           T  FFF+LL    LI +   +PNY++AL+KS+LFF+GQRSG +P GQQI+WR++SGL DG
Sbjct: 2   TSLFFFVLLFSSLLISNGDANPNYKEALSKSLLFFQGQRSGPLPRGQQISWRASSGLSDG 61

Query: 69  ELAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEYGARMGTQLANTRAAIRWATDYL 128
             AHVDLTGGYYDAGDNVKFN PMAFTTTMLSW ALEYG RMG +L N R  IRWATDYL
Sbjct: 62  SAAHVDLTGGYYDAGDNVKFNLPMAFTTTMLSWSALEYGKRMGPELENARVNIRWATDYL 121

Query: 129 LKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAGETAAALAAA 188
           LKCA ATPGKLYVGVGDPNVDHKCWERPEDMDT RTVYSVSA NPGSDVA ETAAALAAA
Sbjct: 122 LKCARATPGKLYVGVGDPNVDHKCWERPEDMDTPRTVYSVSASNPGSDVAAETAAALAAA 181

Query: 189 SLVFRRVDRKYSGLLLATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGYKDELVWGAT 248
           S+VFR+VD KYS LLLATAK V+QFA++++G+YSDSL S+VCPFYCSYSGYKDEL+WGA+
Sbjct: 182 SMVFRKVDSKYSRLLLATAKDVMQFAIQYQGAYSDSLSSSVCPFYCSYSGYKDELMWGAS 241

Query: 249 WLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNFDSYKQEAE 308
           WLLRATN+  Y N +KSLGG D  DIFSWDNKYAGA+VLLSRRALLN D NF+ YKQ AE
Sbjct: 242 WLLRATNNPYYANFIKSLGGGDQPDIFSWDNKYAGAYVLLSRRALLNKDSNFEQYKQAAE 301

Query: 309 AFMCRILPKSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAKHTFNCGSL 368
            F+C+ILP SP SSTQYTQGGLM+KLP+SNLQYVTSITFLLTTY+KYM A KHTFNCGS 
Sbjct: 302 NFICKILPDSPSSSTQYTQGGLMYKLPQSNLQYVTSITFLLTTYAKYMKATKHTFNCGSS 361

Query: 369 LVTPASLKNLAKEQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKASHPQAIGCD 428
           ++ P +L +L+K QVDYILG NP+KMSYMVGF  +FP+RIHHR SSLPS A   Q++GC+
Sbjct: 362 VIVPNALISLSKRQVDYILGDNPIKMSYMVGFSSNFPKRIHHRASSLPSHALRSQSLGCN 421

Query: 429 GGFQPFFYSYNPNPNILTGAVVGEPS 455
           GGFQ  FY+ NPNPNILTGA+VG P+
Sbjct: 422 GGFQS-FYTQNPNPNILTGAIVGGPN 446

BLAST of Spg021475 vs. ExPASy Swiss-Prot
Match: Q2V4L8 (Endoglucanase 3 OS=Arabidopsis thaliana OX=3702 GN=CEL5 PE=2 SV=2)

HSP 1 Score: 694.5 bits (1791), Expect = 8.6e-199
Identity = 327/443 (73.81%), Postives = 381/443 (86.00%), Query Frame = 0

Query: 12  FFFLLLLPFSLIGSAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITWRSNSGLYDGELA 71
           FFF+ LL    + + +  PNYR+AL+KS+LFF+GQRSGR+P+ QQ++WRS+SGL DG  A
Sbjct: 5   FFFVFLLSALSLENTYASPNYREALSKSLLFFQGQRSGRLPSDQQLSWRSSSGLSDGSSA 64

Query: 72  HVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEYGARMGTQLANTRAAIRWATDYLLKC 131
           HVDLTGGYYDAGDNVKFNFPMAFTTTMLSW +LEYG +MG +L N+R AIRWATDYLLKC
Sbjct: 65  HVDLTGGYYDAGDNVKFNFPMAFTTTMLSWSSLEYGKKMGPELQNSRVAIRWATDYLLKC 124

Query: 132 ATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAGETAAALAAASLV 191
           A ATPGKLYVGVGDPN DHKCWERPEDMDT RTVYSVS  NPGSDVA ETAAALAA+S+V
Sbjct: 125 ARATPGKLYVGVGDPNGDHKCWERPEDMDTPRTVYSVSPSNPGSDVAAETAAALAASSMV 184

Query: 192 FRRVDRKYSGLLLATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGYKDELVWGATWLL 251
           FR+VD KYS LLLATAKKV+QFA+++RG+YS+SL S+VCPFYCSYSGYKDEL+WGA WL 
Sbjct: 185 FRKVDPKYSRLLLATAKKVMQFAIQYRGAYSNSLSSSVCPFYCSYSGYKDELLWGAAWLH 244

Query: 252 RATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNFDSYKQEAEAFM 311
           RATND  Y N +KSLGG D  DIFSWDNKYAGA+VLLSRRA+LN D NF+ YKQ AE FM
Sbjct: 245 RATNDPYYTNFIKSLGGGDQPDIFSWDNKYAGAYVLLSRRAVLNKDNNFELYKQAAENFM 304

Query: 312 CRILPKSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAKHTFNCGSLLVT 371
           C+ILP SP SST+YT+GGLM+KLP+SNLQYVTSITFLLTTY+KYM + K TFNCG+ L+ 
Sbjct: 305 CKILPNSPSSSTKYTKGGLMYKLPQSNLQYVTSITFLLTTYAKYMKSTKQTFNCGNSLIV 364

Query: 372 PASLKNLAKEQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKASHPQAIGCDGGF 431
           P +L NL+K QVDY+LGVNP+KMSYMVGF  +FP+RIHHRGSSLPS+A    ++GC+GGF
Sbjct: 365 PNALINLSKRQVDYVLGVNPMKMSYMVGFSSNFPKRIHHRGSSLPSRAVRSNSLGCNGGF 424

Query: 432 QPFFYSYNPNPNILTGAVVGEPS 455
           Q  F + NPNPNILTGA+VG P+
Sbjct: 425 QS-FRTQNPNPNILTGAIVGGPN 446

BLAST of Spg021475 vs. ExPASy Swiss-Prot
Match: Q7XTH4 (Endoglucanase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU4 PE=2 SV=3)

HSP 1 Score: 577.8 bits (1488), Expect = 1.2e-163
Identity = 278/430 (64.65%), Postives = 333/430 (77.44%), Query Frame = 0

Query: 25  SAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITWRSNSGLYDGELAHVDLTGGYYDAGD 84
           +A G P+Y DALAKSILFF+GQRSGR+P  Q + WRSNSGL DG  A+VDLTGGYYD GD
Sbjct: 32  AAAGHPDYADALAKSILFFQGQRSGRLPPDQAVKWRSNSGLSDGSAANVDLTGGYYDGGD 91

Query: 85  NVKFNFPMAFTTTMLSWGALEYGARM-GTQLANTRAAIRWATDYLLKCATATPGKLYVGV 144
           NVKF FPMAFTTTMLSWG +EYG RM G  L + R A+RWA DYLL+ ATATPG LYVGV
Sbjct: 92  NVKFGFPMAFTTTMLSWGVVEYGGRMRGRVLRDARDAVRWAADYLLRAATATPGVLYVGV 151

Query: 145 GDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAGETAAALAAASLVFRRVDRKYSGLL 204
           GDP+ DH+CWERPEDMDT R VYSVSA +PGSDVA ETAAALAAASL  R  D  YS  L
Sbjct: 152 GDPDADHRCWERPEDMDTPRAVYSVSASSPGSDVAAETAAALAAASLALRAADPGYSRRL 211

Query: 205 LATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGYKDELVWGATWLLRATNDVQYFNLL 264
           LA A+ V+ FAV H+G YSD +G  V  +Y SYSGY+DEL+WG+ WLL AT +  Y + L
Sbjct: 212 LAAARDVMAFAVRHQGKYSDHVGGDVGAYYASYSGYQDELLWGSAWLLWATRNASYLDYL 271

Query: 265 KSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNFDSYKQEAEAFMCRILPKSPYSST 324
            SLG +D  D+FSWDNK AGA VLLSRRAL+N D+  D++++ AE F+CRILP SP S+T
Sbjct: 272 ASLGANDGVDMFSWDNKLAGARVLLSRRALVNGDRRLDAFRRLAEDFICRILPGSPSSTT 331

Query: 325 QYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAKHTFNCGSLLVTPASLKNLAKEQV 384
           QYT GG+M+K   +NLQYVTS +FLLTT++KYM+ + HTF+C SL VT  +L+ LA++QV
Sbjct: 332 QYTPGGMMYKSGHANLQYVTSASFLLTTFAKYMAVSNHTFSCQSLPVTAKTLRALARKQV 391

Query: 385 DYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKASHPQAIGCDGGFQPFFYSYNPNPN 444
           DYILG NP  MSYMVG+G  FP+RIHHRG+S+PS A++P  IGC  GF  +F +   NPN
Sbjct: 392 DYILGANPQGMSYMVGYGARFPQRIHHRGASMPSVAAYPAHIGCQEGFSGYFNAGGANPN 451

Query: 445 ILTGAVVGEP 454
           + TGAVVG P
Sbjct: 452 VHTGAVVGGP 461

BLAST of Spg021475 vs. ExPASy Swiss-Prot
Match: Q9SRX3 (Endoglucanase 1 OS=Arabidopsis thaliana OX=3702 GN=CEL2 PE=2 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 4.2e-153
Identity = 261/427 (61.12%), Postives = 325/427 (76.11%), Query Frame = 0

Query: 31  NYRDALAKSILFFEGQRSGRIPAGQQITWRSNSGLYDGELAHVDLTGGYYDAGDNVKFNF 90
           NY+DAL+KSILFFEGQRSG++P  Q++TWRSNSGL DG   +VDL GGYYDAGDN+KF F
Sbjct: 43  NYKDALSKSILFFEGQRSGKLPPNQRMTWRSNSGLSDGSALNVDLVGGYYDAGDNMKFGF 102

Query: 91  PMAFTTTMLSWGALEYGARMGTQLANTRAAIRWATDYLLKCATATPGKLYVGVGDPNVDH 150
           PMAFTTTMLSW  +E+G  M ++L N + AIRWATD+LLK AT+ P  +YV VGDPN+DH
Sbjct: 103 PMAFTTTMLSWSLIEFGGLMKSELPNAKDAIRWATDFLLK-ATSHPDTIYVQVGDPNMDH 162

Query: 151 KCWERPEDMDTVRTVYSVSAGNPGSDVAGETAAALAAASLVFRRVDRKYSGLLLATAKKV 210
            CWERPEDMDT R+V+ V   NPGSD+AGE AAALAAAS+VFR+ D  YS  LL  A  V
Sbjct: 163 ACWERPEDMDTPRSVFKVDKNNPGSDIAGEIAAALAAASIVFRKCDPSYSNHLLQRAITV 222

Query: 211 LQFAVEHRGSYSDSLGSAVCPFYCSYSGYKDELVWGATWLLRATNDVQYFNLLKS----L 270
             FA ++RG YS  L   VCPFYCSYSGY+DEL+WGA WL +ATN+  Y N +K+    L
Sbjct: 223 FTFADKYRGPYSAGLAPEVCPFYCSYSGYQDELLWGAAWLQKATNNPTYLNYIKANGQIL 282

Query: 271 GGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNFDSYKQEAEAFMCRILPKSPYSSTQYT 330
           G D+  ++FSWDNK+ GA +LLS+  L+   K+ + YK+ A++F+C +LP +  SS+QYT
Sbjct: 283 GADEFDNMFSWDNKHVGARILLSKEFLIQKVKSLEEYKEHADSFICSVLPGA--SSSQYT 342

Query: 331 QGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAKHTFNCGSLLVTPASLKNLAKEQVDYI 390
            GGL+FK+ ESN+QYVTS +FLL TY+KY+++A+    CG  +VTPA L+++AK+QVDY+
Sbjct: 343 PGGLLFKMGESNMQYVTSTSFLLLTYAKYLTSARTVAYCGGSVVTPARLRSIAKKQVDYL 402

Query: 391 LGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKASHPQAIGCDGGFQPFFYSYNPNPNILT 450
           LG NPLKMSYMVG+G  +PRRIHHRGSSLPS A HP  I C  GF   F S +PNPN L 
Sbjct: 403 LGGNPLKMSYMVGYGLKYPRRIHHRGSSLPSVAVHPTRIQCHDGFS-LFTSQSPNPNDLV 462

Query: 451 GAVVGEP 454
           GAVVG P
Sbjct: 463 GAVVGGP 465

BLAST of Spg021475 vs. ExPASy Swiss-Prot
Match: O81416 (Endoglucanase 17 OS=Arabidopsis thaliana OX=3702 GN=At4g02290 PE=2 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 8.7e-151
Identity = 254/427 (59.48%), Postives = 318/427 (74.47%), Query Frame = 0

Query: 31  NYRDALAKSILFFEGQRSGRIPAGQQITWRSNSGLYDGELAHVDLTGGYYDAGDNVKFNF 90
           NY+DAL KSILFFEGQRSG++P+ Q+++WR +SGL DG   HVDL GGYYDAGDN+KF F
Sbjct: 52  NYKDALTKSILFFEGQRSGKLPSNQRMSWRRDSGLSDGSALHVDLVGGYYDAGDNIKFGF 111

Query: 91  PMAFTTTMLSWGALEYGARMGTQLANTRAAIRWATDYLLKCATATPGKLYVGVGDPNVDH 150
           PMAFTTTMLSW  +E+G  M ++L N + AIRWATDYLLK AT+ P  +YV VGD N DH
Sbjct: 112 PMAFTTTMLSWSVIEFGGLMKSELQNAKIAIRWATDYLLK-ATSQPDTIYVQVGDANKDH 171

Query: 151 KCWERPEDMDTVRTVYSVSAGNPGSDVAGETAAALAAASLVFRRVDRKYSGLLLATAKKV 210
            CWERPEDMDTVR+V+ V    PGSDVA ETAAALAAA++VFR+ D  YS +LL  A  V
Sbjct: 172 SCWERPEDMDTVRSVFKVDKNIPGSDVAAETAAALAAAAIVFRKSDPSYSKVLLKRAISV 231

Query: 211 LQFAVEHRGSYSDSLGSAVCPFYCSYSGYKDELVWGATWLLRATNDVQYFNLLK----SL 270
             FA ++RG+YS  L   VCPFYCSYSGY+DEL+WGA WL +AT +++Y N +K     L
Sbjct: 232 FAFADKYRGTYSAGLKPDVCPFYCSYSGYQDELLWGAAWLQKATKNIKYLNYIKINGQIL 291

Query: 271 GGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNFDSYKQEAEAFMCRILPKSPYSSTQYT 330
           G  +  + F WDNK+AGA +LL++  L+ N K    YK  A+ F+C ++P +P+SSTQYT
Sbjct: 292 GAAEYDNTFGWDNKHAGARILLTKAFLVQNVKTLHEYKGHADNFICSVIPGAPFSSTQYT 351

Query: 331 QGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAKHTFNCGSLLVTPASLKNLAKEQVDYI 390
            GGL+FK+ ++N+QYVTS +FLL TY+KY+++AK   +CG  + TP  L+++AK QVDY+
Sbjct: 352 PGGLLFKMADANMQYVTSTSFLLLTYAKYLTSAKTVVHCGGSVYTPGRLRSIAKRQVDYL 411

Query: 391 LGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKASHPQAIGCDGGFQPFFYSYNPNPNILT 450
           LG NPL+MSYMVG+G  FPRRIHHRGSSLP  ASHP  I C  GF     S +PNPN L 
Sbjct: 412 LGDNPLRMSYMVGYGPKFPRRIHHRGSSLPCVASHPAKIQCHQGF-AIMNSQSPNPNFLV 471

Query: 451 GAVVGEP 454
           GAVVG P
Sbjct: 472 GAVVGGP 476

BLAST of Spg021475 vs. ExPASy TrEMBL
Match: A0A6J1F1W4 (Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111438904 PE=3 SV=1)

HSP 1 Score: 858.2 bits (2216), Expect = 1.7e-245
Identity = 416/454 (91.63%), Postives = 432/454 (95.15%), Query Frame = 0

Query: 1   MAAITNSSTFFFFFLLLLPFSLIGSAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITWR 60
           MAA TN+ TFFFF LL   FS   +A  +PNYRDALAKS+LFF+GQRSGRIP GQQI WR
Sbjct: 1   MAATTNAPTFFFFLLLSSSFSFY-TARANPNYRDALAKSLLFFQGQRSGRIPNGQQIAWR 60

Query: 61  SNSGLYDGELAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEYGARMGTQLANTRAA 120
           SNSGLYDGELAHVDLTGGYYDAGDNVKFN PMAFTTTMLSWGALEYGARMG++L NTRAA
Sbjct: 61  SNSGLYDGELAHVDLTGGYYDAGDNVKFNLPMAFTTTMLSWGALEYGARMGSELPNTRAA 120

Query: 121 IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAGE 180
           IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSA NPGSDVAGE
Sbjct: 121 IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAANPGSDVAGE 180

Query: 181 TAAALAAASLVFRRVDRKYSGLLLATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGYK 240
           TAAALAAASLVFRRVDRKYSGLLLATAKKV QFAVEHRGSYSDSLGSAVCPFYCSYSGYK
Sbjct: 181 TAAALAAASLVFRRVDRKYSGLLLATAKKVFQFAVEHRGSYSDSLGSAVCPFYCSYSGYK 240

Query: 241 DELVWGATWLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNF 300
           DELVWGATWLLRATNDV+YFNLLKSLGGDDV DIFSWDNKYAGAHVLLSRRALLNNDKNF
Sbjct: 241 DELVWGATWLLRATNDVRYFNLLKSLGGDDVPDIFSWDNKYAGAHVLLSRRALLNNDKNF 300

Query: 301 DSYKQEAEAFMCRILPKSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAK 360
           DSYKQ+AE+FMCRILP SPYSSTQYTQGGLMFKLP+SNLQYVTSITFLLTTYSKYMSAAK
Sbjct: 301 DSYKQKAESFMCRILPNSPYSSTQYTQGGLMFKLPQSNLQYVTSITFLLTTYSKYMSAAK 360

Query: 361 HTFNCGSLLVTPASLKNLAKEQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKAS 420
           HTFNCG +LVTP SLKNLAK+QVDYILGVNPLKMSYMVGFGK+FP+RIHHRGSSLPSKAS
Sbjct: 361 HTFNCGGVLVTPTSLKNLAKQQVDYILGVNPLKMSYMVGFGKNFPKRIHHRGSSLPSKAS 420

Query: 421 HPQAIGCDGGFQPFFYSYNPNPNILTGAVVGEPS 455
           HPQAIGCDGGFQPFFYSYNPNPNILTGAVVG P+
Sbjct: 421 HPQAIGCDGGFQPFFYSYNPNPNILTGAVVGGPN 453

BLAST of Spg021475 vs. ExPASy TrEMBL
Match: A0A6J1ICX3 (Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111471942 PE=3 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 1.4e-244
Identity = 414/454 (91.19%), Postives = 427/454 (94.05%), Query Frame = 0

Query: 1   MAAITNSSTFFFFFLLLLPFSLIGSAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITWR 60
           MA  TN STFFFF  LL          G+PNYRDALAKS+LFF+GQRSGRIP GQQI WR
Sbjct: 1   MATTTNVSTFFFFLFLLSSSFSFNITRGNPNYRDALAKSLLFFQGQRSGRIPNGQQIAWR 60

Query: 61  SNSGLYDGELAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEYGARMGTQLANTRAA 120
           SNSGLYDGELAHVDLTGGYYDAGDNVKFN PMAFTTTMLSWGALEYGARMG++L NTRAA
Sbjct: 61  SNSGLYDGELAHVDLTGGYYDAGDNVKFNLPMAFTTTMLSWGALEYGARMGSELPNTRAA 120

Query: 121 IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAGE 180
           IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSA NPGSDVAGE
Sbjct: 121 IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAANPGSDVAGE 180

Query: 181 TAAALAAASLVFRRVDRKYSGLLLATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGYK 240
           TAAALAAASLVFRRVDRKYSGLLLATAKKV QFAVEHRGSYSDSLGSAVCPFYCSYSGYK
Sbjct: 181 TAAALAAASLVFRRVDRKYSGLLLATAKKVFQFAVEHRGSYSDSLGSAVCPFYCSYSGYK 240

Query: 241 DELVWGATWLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNF 300
           DELVWGATWLLRATNDVQY NLLKSLGGDDV DIFSWDNKYAGAHVLLSRRALLNNDKNF
Sbjct: 241 DELVWGATWLLRATNDVQYINLLKSLGGDDVPDIFSWDNKYAGAHVLLSRRALLNNDKNF 300

Query: 301 DSYKQEAEAFMCRILPKSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAK 360
           DSYKQ AE+FMCRILP SPYSSTQYTQGGLMFKLP+SNLQYVTSITFLLTTYSKYMSAAK
Sbjct: 301 DSYKQTAESFMCRILPNSPYSSTQYTQGGLMFKLPQSNLQYVTSITFLLTTYSKYMSAAK 360

Query: 361 HTFNCGSLLVTPASLKNLAKEQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKAS 420
           HTFNCG LLVTPASLKNLAK+QVDYILGVNPLKMSYMVGFG++FP+RIHHRGSSLPSKAS
Sbjct: 361 HTFNCGGLLVTPASLKNLAKQQVDYILGVNPLKMSYMVGFGRNFPKRIHHRGSSLPSKAS 420

Query: 421 HPQAIGCDGGFQPFFYSYNPNPNILTGAVVGEPS 455
           HPQAIGCDGGFQPFFYS+NPNPNILTGAVVG P+
Sbjct: 421 HPQAIGCDGGFQPFFYSFNPNPNILTGAVVGGPN 454

BLAST of Spg021475 vs. ExPASy TrEMBL
Match: A0A6J1CKC4 (Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111012089 PE=3 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 2.6e-243
Identity = 413/455 (90.77%), Postives = 430/455 (94.51%), Query Frame = 0

Query: 1   MAAITNSSTFFFFF-LLLLPFSLIGSAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITW 60
           MAA   +ST FF F LLL PFS +  A GDPNYRDALAKSILFF+GQRSGRIPAG QI+W
Sbjct: 1   MAATPKASTLFFLFSLLLFPFSWMDGARGDPNYRDALAKSILFFQGQRSGRIPAGLQISW 60

Query: 61  RSNSGLYDGELAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEYGARMGTQLANTRA 120
           RSNSGLYDGELAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALE+GARMGTQLANTRA
Sbjct: 61  RSNSGLYDGELAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEHGARMGTQLANTRA 120

Query: 121 AIRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAG 180
           AIRWATDYLLKCATATPGK+YVGVGDPNVDH+CWERPEDMDTVRTVYSVSA NPGSDVAG
Sbjct: 121 AIRWATDYLLKCATATPGKVYVGVGDPNVDHRCWERPEDMDTVRTVYSVSAANPGSDVAG 180

Query: 181 ETAAALAAASLVFRRVDRKYSGLLLATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGY 240
           ETAAALAAAS+VFR+VDRKYS LLLATAKKVLQFAV+++GSYSDSLGSAVCPFYCSYSGY
Sbjct: 181 ETAAALAAASMVFRKVDRKYSNLLLATAKKVLQFAVQYKGSYSDSLGSAVCPFYCSYSGY 240

Query: 241 KDELVWGATWLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKN 300
           KDELVWGA WLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLN DKN
Sbjct: 241 KDELVWGAAWLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNKDKN 300

Query: 301 FDSYKQEAEAFMCRILPKSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAA 360
           FDSYKQEAEAFMCRILP SPYSST YTQGGLMFKLPESNLQYVTSITFLL TYSKYMSAA
Sbjct: 301 FDSYKQEAEAFMCRILPNSPYSSTHYTQGGLMFKLPESNLQYVTSITFLLATYSKYMSAA 360

Query: 361 KHTFNCGSLLVTPASLKNLAKEQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKA 420
           KH+FNCGSLLVTPASLKNLAK+QVDYILG NPLKMSYMVG+G  FPRRIHHRGSSLPSKA
Sbjct: 361 KHSFNCGSLLVTPASLKNLAKKQVDYILGENPLKMSYMVGYGPHFPRRIHHRGSSLPSKA 420

Query: 421 SHPQAIGCDGGFQPFFYSYNPNPNILTGAVVGEPS 455
           SHPQ IGCDGGFQPFFYSYNPNPN+L GAVVG P+
Sbjct: 421 SHPQTIGCDGGFQPFFYSYNPNPNLLIGAVVGGPN 455

BLAST of Spg021475 vs. ExPASy TrEMBL
Match: A0A0A0KW33 (Endoglucanase OS=Cucumis sativus OX=3659 GN=Csa_5G648680 PE=3 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.7e-240
Identity = 406/453 (89.62%), Postives = 428/453 (94.48%), Query Frame = 0

Query: 2   AAITNSSTFFFFFLLLLPFSLIGSAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITWRS 61
           +AI+NSST F  F LLL FS  G A   PNYRDALAKSILFFEGQRSGRIPA Q+ITWRS
Sbjct: 3   SAISNSSTLFLLFFLLLSFSFAGRALAGPNYRDALAKSILFFEGQRSGRIPANQRITWRS 62

Query: 62  NSGLYDGELAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEYGARMGTQLANTRAAI 121
           NSGLYDGEL HVDLTGGYYDAGDNVKFN PMAFTTTMLSWGALEYGARMG++L NTRAAI
Sbjct: 63  NSGLYDGELDHVDLTGGYYDAGDNVKFNLPMAFTTTMLSWGALEYGARMGSELPNTRAAI 122

Query: 122 RWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAGET 181
           RWATDYLLKCATATPGKLYVGVG+P+ DHKCWERPEDMDTVRTVYSVSAGNPGSDVAGET
Sbjct: 123 RWATDYLLKCATATPGKLYVGVGEPHADHKCWERPEDMDTVRTVYSVSAGNPGSDVAGET 182

Query: 182 AAALAAASLVFRRVDRKYSGLLLATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGYKD 241
           AAALAAASLVFRRVDRKYS +LLATAKKV++FA+EHRGSYSDSL SAVCPFYCSYSGYKD
Sbjct: 183 AAALAAASLVFRRVDRKYSKVLLATAKKVMEFALEHRGSYSDSLSSAVCPFYCSYSGYKD 242

Query: 242 ELVWGATWLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNFD 301
           ELVWGA WLLRATN+V+YFNLLKSLGGDDVTDIFSWDNK+AGAHVLLSRR+LLNNDKNFD
Sbjct: 243 ELVWGAAWLLRATNNVKYFNLLKSLGGDDVTDIFSWDNKFAGAHVLLSRRSLLNNDKNFD 302

Query: 302 SYKQEAEAFMCRILPKSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAKH 361
           SYKQEAEAFMCRILP SP SSTQYTQG LMFKLPESNLQYVTSITFLLTTYSKYMSAAKH
Sbjct: 303 SYKQEAEAFMCRILPNSPSSSTQYTQGRLMFKLPESNLQYVTSITFLLTTYSKYMSAAKH 362

Query: 362 TFNCGSLLVTPASLKNLAKEQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKASH 421
           TFNCG+L+VTPASLKNLAK QVDYILGVNPLKMSYMVGFGK++P+RIHHRGSSLPSKA+H
Sbjct: 363 TFNCGNLVVTPASLKNLAKIQVDYILGVNPLKMSYMVGFGKNYPKRIHHRGSSLPSKATH 422

Query: 422 PQAIGCDGGFQPFFYSYNPNPNILTGAVVGEPS 455
           PQAI CDGGFQPFFYSYNPNPNILTGAVVG P+
Sbjct: 423 PQAIACDGGFQPFFYSYNPNPNILTGAVVGGPN 455

BLAST of Spg021475 vs. ExPASy TrEMBL
Match: A0A5A7TDF3 (Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00910 PE=3 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 1.3e-237
Identity = 405/454 (89.21%), Postives = 427/454 (94.05%), Query Frame = 0

Query: 4   ITNSST---FFFFFLLLLPFSLIGSAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITWR 63
           I+NSS+     FFF LLL FS  G A  +PNYRDALAKSILFFEGQRSGRIPA Q+ITWR
Sbjct: 5   ISNSSSTLYSLFFFGLLLSFSFAGRARANPNYRDALAKSILFFEGQRSGRIPANQRITWR 64

Query: 64  SNSGLYDGELAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEYGARMGTQLANTRAA 123
           SNSGLYDGELAHVDLTGGYYDAGDNVKFN PMAFTTTMLSWGALEYGARMG++L NTRAA
Sbjct: 65  SNSGLYDGELAHVDLTGGYYDAGDNVKFNLPMAFTTTMLSWGALEYGARMGSELGNTRAA 124

Query: 124 IRWATDYLLKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAGE 183
           IRWATDYLLKCATATPGKLYVGVGDP+ DHKCWERPEDMDTVRTVYSVSAGNPGSDVAGE
Sbjct: 125 IRWATDYLLKCATATPGKLYVGVGDPHADHKCWERPEDMDTVRTVYSVSAGNPGSDVAGE 184

Query: 184 TAAALAAASLVFRRVDRKYSGLLLATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGYK 243
           TAAALAAASLVFRRVDRKYS +LLATAKKV++FA+EHRGSYSDSL SAVCPFYCSYSGYK
Sbjct: 185 TAAALAAASLVFRRVDRKYSRVLLATAKKVMEFALEHRGSYSDSLSSAVCPFYCSYSGYK 244

Query: 244 DELVWGATWLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNF 303
           DELVWGA WLLRATNDV+YFNLLKSLGGDDVTDIFSWDNK+AGAHVLLSRR+LLNNDKNF
Sbjct: 245 DELVWGAAWLLRATNDVKYFNLLKSLGGDDVTDIFSWDNKFAGAHVLLSRRSLLNNDKNF 304

Query: 304 DSYKQEAEAFMCRILPKSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAK 363
           D YKQEAEAFMCRILP SP SST+YTQG LMFKLPESNLQYVTSITFLLTTYSKYMSAAK
Sbjct: 305 DLYKQEAEAFMCRILPNSPSSSTKYTQGRLMFKLPESNLQYVTSITFLLTTYSKYMSAAK 364

Query: 364 HTFNCGSLLVTPASLKNLAKEQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKAS 423
           HTFNCG+L+VTPASLKNLAK QVDYILGVNPLKMSYMVG+GK+FP+RIHHRGSSLPSKA+
Sbjct: 365 HTFNCGNLVVTPASLKNLAKIQVDYILGVNPLKMSYMVGYGKNFPKRIHHRGSSLPSKAT 424

Query: 424 HPQAIGCDGGFQPFFYSYNPNPNILTGAVVGEPS 455
           HPQAI CDGGFQPFFYSYNPNPNIL GAVVG P+
Sbjct: 425 HPQAIACDGGFQPFFYSYNPNPNILIGAVVGGPN 458

BLAST of Spg021475 vs. TAIR 10
Match: AT1G71380.1 (cellulase 3 )

HSP 1 Score: 702.6 bits (1812), Expect = 2.2e-202
Identity = 335/446 (75.11%), Postives = 382/446 (85.65%), Query Frame = 0

Query: 9   TFFFFFLLLLPFSLIGSAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITWRSNSGLYDG 68
           T  FFF+LL    LI +   +PNY++AL+KS+LFF+GQRSG +P GQQI+WR++SGL DG
Sbjct: 2   TSLFFFVLLFSSLLISNGDANPNYKEALSKSLLFFQGQRSGPLPRGQQISWRASSGLSDG 61

Query: 69  ELAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEYGARMGTQLANTRAAIRWATDYL 128
             AHVDLTGGYYDAGDNVKFN PMAFTTTMLSW ALEYG RMG +L N R  IRWATDYL
Sbjct: 62  SAAHVDLTGGYYDAGDNVKFNLPMAFTTTMLSWSALEYGKRMGPELENARVNIRWATDYL 121

Query: 129 LKCATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAGETAAALAAA 188
           LKCA ATPGKLYVGVGDPNVDHKCWERPEDMDT RTVYSVSA NPGSDVA ETAAALAAA
Sbjct: 122 LKCARATPGKLYVGVGDPNVDHKCWERPEDMDTPRTVYSVSASNPGSDVAAETAAALAAA 181

Query: 189 SLVFRRVDRKYSGLLLATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGYKDELVWGAT 248
           S+VFR+VD KYS LLLATAK V+QFA++++G+YSDSL S+VCPFYCSYSGYKDEL+WGA+
Sbjct: 182 SMVFRKVDSKYSRLLLATAKDVMQFAIQYQGAYSDSLSSSVCPFYCSYSGYKDELMWGAS 241

Query: 249 WLLRATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNFDSYKQEAE 308
           WLLRATN+  Y N +KSLGG D  DIFSWDNKYAGA+VLLSRRALLN D NF+ YKQ AE
Sbjct: 242 WLLRATNNPYYANFIKSLGGGDQPDIFSWDNKYAGAYVLLSRRALLNKDSNFEQYKQAAE 301

Query: 309 AFMCRILPKSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAKHTFNCGSL 368
            F+C+ILP SP SSTQYTQGGLM+KLP+SNLQYVTSITFLLTTY+KYM A KHTFNCGS 
Sbjct: 302 NFICKILPDSPSSSTQYTQGGLMYKLPQSNLQYVTSITFLLTTYAKYMKATKHTFNCGSS 361

Query: 369 LVTPASLKNLAKEQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKASHPQAIGCD 428
           ++ P +L +L+K QVDYILG NP+KMSYMVGF  +FP+RIHHR SSLPS A   Q++GC+
Sbjct: 362 VIVPNALISLSKRQVDYILGDNPIKMSYMVGFSSNFPKRIHHRASSLPSHALRSQSLGCN 421

Query: 429 GGFQPFFYSYNPNPNILTGAVVGEPS 455
           GGFQ  FY+ NPNPNILTGA+VG P+
Sbjct: 422 GGFQS-FYTQNPNPNILTGAIVGGPN 446

BLAST of Spg021475 vs. TAIR 10
Match: AT1G22880.1 (cellulase 5 )

HSP 1 Score: 694.5 bits (1791), Expect = 6.1e-200
Identity = 327/443 (73.81%), Postives = 381/443 (86.00%), Query Frame = 0

Query: 12  FFFLLLLPFSLIGSAWGDPNYRDALAKSILFFEGQRSGRIPAGQQITWRSNSGLYDGELA 71
           FFF+ LL    + + +  PNYR+AL+KS+LFF+GQRSGR+P+ QQ++WRS+SGL DG  A
Sbjct: 5   FFFVFLLSALSLENTYASPNYREALSKSLLFFQGQRSGRLPSDQQLSWRSSSGLSDGSSA 64

Query: 72  HVDLTGGYYDAGDNVKFNFPMAFTTTMLSWGALEYGARMGTQLANTRAAIRWATDYLLKC 131
           HVDLTGGYYDAGDNVKFNFPMAFTTTMLSW +LEYG +MG +L N+R AIRWATDYLLKC
Sbjct: 65  HVDLTGGYYDAGDNVKFNFPMAFTTTMLSWSSLEYGKKMGPELQNSRVAIRWATDYLLKC 124

Query: 132 ATATPGKLYVGVGDPNVDHKCWERPEDMDTVRTVYSVSAGNPGSDVAGETAAALAAASLV 191
           A ATPGKLYVGVGDPN DHKCWERPEDMDT RTVYSVS  NPGSDVA ETAAALAA+S+V
Sbjct: 125 ARATPGKLYVGVGDPNGDHKCWERPEDMDTPRTVYSVSPSNPGSDVAAETAAALAASSMV 184

Query: 192 FRRVDRKYSGLLLATAKKVLQFAVEHRGSYSDSLGSAVCPFYCSYSGYKDELVWGATWLL 251
           FR+VD KYS LLLATAKKV+QFA+++RG+YS+SL S+VCPFYCSYSGYKDEL+WGA WL 
Sbjct: 185 FRKVDPKYSRLLLATAKKVMQFAIQYRGAYSNSLSSSVCPFYCSYSGYKDELLWGAAWLH 244

Query: 252 RATNDVQYFNLLKSLGGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNFDSYKQEAEAFM 311
           RATND  Y N +KSLGG D  DIFSWDNKYAGA+VLLSRRA+LN D NF+ YKQ AE FM
Sbjct: 245 RATNDPYYTNFIKSLGGGDQPDIFSWDNKYAGAYVLLSRRAVLNKDNNFELYKQAAENFM 304

Query: 312 CRILPKSPYSSTQYTQGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAKHTFNCGSLLVT 371
           C+ILP SP SST+YT+GGLM+KLP+SNLQYVTSITFLLTTY+KYM + K TFNCG+ L+ 
Sbjct: 305 CKILPNSPSSSTKYTKGGLMYKLPQSNLQYVTSITFLLTTYAKYMKSTKQTFNCGNSLIV 364

Query: 372 PASLKNLAKEQVDYILGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKASHPQAIGCDGGF 431
           P +L NL+K QVDY+LGVNP+KMSYMVGF  +FP+RIHHRGSSLPS+A    ++GC+GGF
Sbjct: 365 PNALINLSKRQVDYVLGVNPMKMSYMVGFSSNFPKRIHHRGSSLPSRAVRSNSLGCNGGF 424

Query: 432 QPFFYSYNPNPNILTGAVVGEPS 455
           Q  F + NPNPNILTGA+VG P+
Sbjct: 425 QS-FRTQNPNPNILTGAIVGGPN 446

BLAST of Spg021475 vs. TAIR 10
Match: AT1G22880.2 (cellulase 5 )

HSP 1 Score: 577.0 bits (1486), Expect = 1.4e-164
Identity = 273/363 (75.21%), Postives = 314/363 (86.50%), Query Frame = 0

Query: 92  MAFTTTMLSWGALEYGARMGTQLANTRAAIRWATDYLLKCATATPGKLYVGVGDPNVDHK 151
           MAFTTTMLSW +LEYG +MG +L N+R AIRWATDYLLKCA ATPGKLYVGVGDPN DHK
Sbjct: 1   MAFTTTMLSWSSLEYGKKMGPELQNSRVAIRWATDYLLKCARATPGKLYVGVGDPNGDHK 60

Query: 152 CWERPEDMDTVRTVYSVSAGNPGSDVAGETAAALAAASLVFRRVDRKYSGLLLATAKKVL 211
           CWERPEDMDT RTVYSVS  NPGSDVA ETAAALAA+S+VFR+VD KYS LLLATAKKV+
Sbjct: 61  CWERPEDMDTPRTVYSVSPSNPGSDVAAETAAALAASSMVFRKVDPKYSRLLLATAKKVM 120

Query: 212 QFAVEHRGSYSDSLGSAVCPFYCSYSGYKDELVWGATWLLRATNDVQYFNLLKSLGGDDV 271
           QFA+++RG+YS+SL S+VCPFYCSYSGYKDEL+WGA WL RATND  Y N +KSLGG D 
Sbjct: 121 QFAIQYRGAYSNSLSSSVCPFYCSYSGYKDELLWGAAWLHRATNDPYYTNFIKSLGGGDQ 180

Query: 272 TDIFSWDNKYAGAHVLLSRRALLNNDKNFDSYKQEAEAFMCRILPKSPYSSTQYTQGGLM 331
            DIFSWDNKYAGA+VLLSRRA+LN D NF+ YKQ AE FMC+ILP SP SST+YT+GGLM
Sbjct: 181 PDIFSWDNKYAGAYVLLSRRAVLNKDNNFELYKQAAENFMCKILPNSPSSSTKYTKGGLM 240

Query: 332 FKLPESNLQYVTSITFLLTTYSKYMSAAKHTFNCGSLLVTPASLKNLAKEQVDYILGVNP 391
           +KLP+SNLQYVTSITFLLTTY+KYM + K TFNCG+ L+ P +L NL+K QVDY+LGVNP
Sbjct: 241 YKLPQSNLQYVTSITFLLTTYAKYMKSTKQTFNCGNSLIVPNALINLSKRQVDYVLGVNP 300

Query: 392 LKMSYMVGFGKSFPRRIHHRGSSLPSKASHPQAIGCDGGFQPFFYSYNPNPNILTGAVVG 451
           +KMSYMVGF  +FP+RIHHRGSSLPS+A    ++GC+GGFQ  F + NPNPNILTGA+VG
Sbjct: 301 MKMSYMVGFSSNFPKRIHHRGSSLPSRAVRSNSLGCNGGFQS-FRTQNPNPNILTGAIVG 360

Query: 452 EPS 455
            P+
Sbjct: 361 GPN 362

BLAST of Spg021475 vs. TAIR 10
Match: AT1G02800.1 (cellulase 2 )

HSP 1 Score: 542.7 bits (1397), Expect = 3.0e-154
Identity = 261/427 (61.12%), Postives = 325/427 (76.11%), Query Frame = 0

Query: 31  NYRDALAKSILFFEGQRSGRIPAGQQITWRSNSGLYDGELAHVDLTGGYYDAGDNVKFNF 90
           NY+DAL+KSILFFEGQRSG++P  Q++TWRSNSGL DG   +VDL GGYYDAGDN+KF F
Sbjct: 43  NYKDALSKSILFFEGQRSGKLPPNQRMTWRSNSGLSDGSALNVDLVGGYYDAGDNMKFGF 102

Query: 91  PMAFTTTMLSWGALEYGARMGTQLANTRAAIRWATDYLLKCATATPGKLYVGVGDPNVDH 150
           PMAFTTTMLSW  +E+G  M ++L N + AIRWATD+LLK AT+ P  +YV VGDPN+DH
Sbjct: 103 PMAFTTTMLSWSLIEFGGLMKSELPNAKDAIRWATDFLLK-ATSHPDTIYVQVGDPNMDH 162

Query: 151 KCWERPEDMDTVRTVYSVSAGNPGSDVAGETAAALAAASLVFRRVDRKYSGLLLATAKKV 210
            CWERPEDMDT R+V+ V   NPGSD+AGE AAALAAAS+VFR+ D  YS  LL  A  V
Sbjct: 163 ACWERPEDMDTPRSVFKVDKNNPGSDIAGEIAAALAAASIVFRKCDPSYSNHLLQRAITV 222

Query: 211 LQFAVEHRGSYSDSLGSAVCPFYCSYSGYKDELVWGATWLLRATNDVQYFNLLKS----L 270
             FA ++RG YS  L   VCPFYCSYSGY+DEL+WGA WL +ATN+  Y N +K+    L
Sbjct: 223 FTFADKYRGPYSAGLAPEVCPFYCSYSGYQDELLWGAAWLQKATNNPTYLNYIKANGQIL 282

Query: 271 GGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNFDSYKQEAEAFMCRILPKSPYSSTQYT 330
           G D+  ++FSWDNK+ GA +LLS+  L+   K+ + YK+ A++F+C +LP +  SS+QYT
Sbjct: 283 GADEFDNMFSWDNKHVGARILLSKEFLIQKVKSLEEYKEHADSFICSVLPGA--SSSQYT 342

Query: 331 QGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAKHTFNCGSLLVTPASLKNLAKEQVDYI 390
            GGL+FK+ ESN+QYVTS +FLL TY+KY+++A+    CG  +VTPA L+++AK+QVDY+
Sbjct: 343 PGGLLFKMGESNMQYVTSTSFLLLTYAKYLTSARTVAYCGGSVVTPARLRSIAKKQVDYL 402

Query: 391 LGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKASHPQAIGCDGGFQPFFYSYNPNPNILT 450
           LG NPLKMSYMVG+G  +PRRIHHRGSSLPS A HP  I C  GF   F S +PNPN L 
Sbjct: 403 LGGNPLKMSYMVGYGLKYPRRIHHRGSSLPSVAVHPTRIQCHDGFS-LFTSQSPNPNDLV 462

Query: 451 GAVVGEP 454
           GAVVG P
Sbjct: 463 GAVVGGP 465

BLAST of Spg021475 vs. TAIR 10
Match: AT4G02290.1 (glycosyl hydrolase 9B13 )

HSP 1 Score: 535.0 bits (1377), Expect = 6.2e-152
Identity = 254/427 (59.48%), Postives = 318/427 (74.47%), Query Frame = 0

Query: 31  NYRDALAKSILFFEGQRSGRIPAGQQITWRSNSGLYDGELAHVDLTGGYYDAGDNVKFNF 90
           NY+DAL KSILFFEGQRSG++P+ Q+++WR +SGL DG   HVDL GGYYDAGDN+KF F
Sbjct: 52  NYKDALTKSILFFEGQRSGKLPSNQRMSWRRDSGLSDGSALHVDLVGGYYDAGDNIKFGF 111

Query: 91  PMAFTTTMLSWGALEYGARMGTQLANTRAAIRWATDYLLKCATATPGKLYVGVGDPNVDH 150
           PMAFTTTMLSW  +E+G  M ++L N + AIRWATDYLLK AT+ P  +YV VGD N DH
Sbjct: 112 PMAFTTTMLSWSVIEFGGLMKSELQNAKIAIRWATDYLLK-ATSQPDTIYVQVGDANKDH 171

Query: 151 KCWERPEDMDTVRTVYSVSAGNPGSDVAGETAAALAAASLVFRRVDRKYSGLLLATAKKV 210
            CWERPEDMDTVR+V+ V    PGSDVA ETAAALAAA++VFR+ D  YS +LL  A  V
Sbjct: 172 SCWERPEDMDTVRSVFKVDKNIPGSDVAAETAAALAAAAIVFRKSDPSYSKVLLKRAISV 231

Query: 211 LQFAVEHRGSYSDSLGSAVCPFYCSYSGYKDELVWGATWLLRATNDVQYFNLLK----SL 270
             FA ++RG+YS  L   VCPFYCSYSGY+DEL+WGA WL +AT +++Y N +K     L
Sbjct: 232 FAFADKYRGTYSAGLKPDVCPFYCSYSGYQDELLWGAAWLQKATKNIKYLNYIKINGQIL 291

Query: 271 GGDDVTDIFSWDNKYAGAHVLLSRRALLNNDKNFDSYKQEAEAFMCRILPKSPYSSTQYT 330
           G  +  + F WDNK+AGA +LL++  L+ N K    YK  A+ F+C ++P +P+SSTQYT
Sbjct: 292 GAAEYDNTFGWDNKHAGARILLTKAFLVQNVKTLHEYKGHADNFICSVIPGAPFSSTQYT 351

Query: 331 QGGLMFKLPESNLQYVTSITFLLTTYSKYMSAAKHTFNCGSLLVTPASLKNLAKEQVDYI 390
            GGL+FK+ ++N+QYVTS +FLL TY+KY+++AK   +CG  + TP  L+++AK QVDY+
Sbjct: 352 PGGLLFKMADANMQYVTSTSFLLLTYAKYLTSAKTVVHCGGSVYTPGRLRSIAKRQVDYL 411

Query: 391 LGVNPLKMSYMVGFGKSFPRRIHHRGSSLPSKASHPQAIGCDGGFQPFFYSYNPNPNILT 450
           LG NPL+MSYMVG+G  FPRRIHHRGSSLP  ASHP  I C  GF     S +PNPN L 
Sbjct: 412 LGDNPLRMSYMVGYGPKFPRRIHHRGSSLPCVASHPAKIQCHQGF-AIMNSQSPNPNFLV 471

Query: 451 GAVVGEP 454
           GAVVG P
Sbjct: 472 GAVVGGP 476

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890642.17.1e-25193.82endoglucanase 9-like [Benincasa hispida][more]
XP_023523831.11.2e-24591.63endoglucanase 9-like [Cucurbita pepo subsp. pepo][more]
KAG6607530.13.4e-24591.41Endoglucanase 9, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022932503.13.4e-24591.63endoglucanase 9-like [Cucurbita moschata][more]
KAG7037172.14.5e-24591.65Endoglucanase 9, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q9C9H53.1e-20175.11Endoglucanase 9 OS=Arabidopsis thaliana OX=3702 GN=CEL3 PE=1 SV=1[more]
Q2V4L88.6e-19973.81Endoglucanase 3 OS=Arabidopsis thaliana OX=3702 GN=CEL5 PE=2 SV=2[more]
Q7XTH41.2e-16364.65Endoglucanase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU4 PE=2 SV=3[more]
Q9SRX34.2e-15361.12Endoglucanase 1 OS=Arabidopsis thaliana OX=3702 GN=CEL2 PE=2 SV=1[more]
O814168.7e-15159.48Endoglucanase 17 OS=Arabidopsis thaliana OX=3702 GN=At4g02290 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F1W41.7e-24591.63Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111438904 PE=3 SV=1[more]
A0A6J1ICX31.4e-24491.19Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111471942 PE=3 SV=1[more]
A0A6J1CKC42.6e-24390.77Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111012089 PE=3 SV=1[more]
A0A0A0KW332.7e-24089.62Endoglucanase OS=Cucumis sativus OX=3659 GN=Csa_5G648680 PE=3 SV=1[more]
A0A5A7TDF31.3e-23789.21Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00910 ... [more]
Match NameE-valueIdentityDescription
AT1G71380.12.2e-20275.11cellulase 3 [more]
AT1G22880.16.1e-20073.81cellulase 5 [more]
AT1G22880.21.4e-16475.21cellulase 5 [more]
AT1G02800.13.0e-15461.12cellulase 2 [more]
AT4G02290.16.2e-15259.48glycosyl hydrolase 9B13 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 29..458
e-value: 3.3E-143
score: 479.8
IPR001701Glycoside hydrolase family 9PFAMPF00759Glyco_hydro_9coord: 32..454
e-value: 1.0E-124
score: 417.3
NoneNo IPR availablePANTHERPTHR22298ENDO-1,4-BETA-GLUCANASEcoord: 17..455
NoneNo IPR availablePANTHERPTHR22298:SF54ENDOGLUCANASE 9coord: 17..455
IPR018221Glycoside hydrolase family 9, His active sitePROSITEPS00592GH9_2coord: 385..411
IPR008928Six-hairpin glycosidase superfamilySUPERFAMILY48208Six-hairpin glycosidasescoord: 28..454

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg021475.1Spg021475.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030245 cellulose catabolic process
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0008810 cellulase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds