Spg021321 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg021321
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein-tyrosine-phosphatase
Locationscaffold6: 46388657 .. 46392695 (+)
RNA-Seq ExpressionSpg021321
SyntenySpg021321
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGATAAGCTAGAGGATCAAAAAGAGGCCCAAAATAAACAAACCAGGCCTTATTCTTTAGCCCATTACAAACATCTGAAACGACGTCGTAACAAGCACTCTTGCCTGCCTCCGACTTGTTCCACTGCTCGTCGTACTTGGACGGAATCAGTGGCTTTTCAGAGCAGAAAGCTCCATTGCTTCACTCCATAGACTCTCTGGTTGACGAAAGTAAGCACCCTTTACGTTTTTATGTTTTAATGTTTCTTATTTCTGAGTTTTTTGTTGGGTAAATGGATCTTGATCATGGCTACTGCTGAAAGTTGGGTTGGAATGGATTTCTGATTCATTGTCTTCTTGCTCTTTTAAATTCTCTTGTTCTTTGTGTTGTGGGTTTCTCTCCATCAGCCATTTTATGGGATTTCTTTGTTGTTTTTGGTTTTGGTTTTAGAATATGATAGATGCATGTTTGTTAAACCTTGTTGGTTTTATCTTGATGCTATTGGGTTTAGACAATGAAGCTTGGTTTGCAAATTAGATGAATCCATTTGAATTACCATAGCTGTGTTCAATGTCTTTGATTGTTACCAAGCTTTACTAAAAATTTTGTTTTGATCAGTTAGATTTTTGTTTTTGTATATGAGCCTTACTTACTAACCATCTAATCTACTTTCGTAATTGTCTTTGAAATTCTAGTCAAATTTTGATAAGTAAAAAGAAACAAGTAGTTCTTAACAAAATGTTTTTTTTTTTAAATTTGACTAAAATTTTGAAAATATTTTTTGAAATGTGATGAACTGAAGCAAATTACATAGAATGTGTTATCCTTAAAAGCTTAATTTTCTTGGAACAAATTTGGAAACTAATCAAATGGAGCCTTAGCCGTGTAGTTTTGTGTTTGAAGTCTCATCAATGTTTTTTTGTCTAGATTTTATTTCCAGAATAAACCAGCACTATGGAAGAAAGTAGAAATCATCATCTTTCTGAACAAAGTACAAAGGGGTTTGCTAAAAGCACCAAGGATCAGAAACTTAATGTGTATGTGTGGGACATGGATGAGACTATCATCCTGCTCAAGTCCTTGTTGAATGGGACATATGCTGAGGCTTTTTGTGGTTCAAAGGATGTTAAAAGGGGTGAAGAACTTGGAAAAATTTGGGAGAAGCAGATTCTTGATCTGTGTGATCATCACTTCTTCTATGAACAAGTAAGGTTGAGATTTCACTCCATAATCATGTTTAAACAACTCCATGATATAATGTCTCAGAAGTTGACGTTTTTGTCATATTTCTATGCAGATAGAGAACTATAATCAGCCCTTTCTTGATGCATTGAGTGAATATGATGATGGCCGTGATCTTTCTAATTATGATTTTGACCAAGATGGTTTTGGTCCACCATATGATGATGGAAACAAAAGAAAACTGGCTTTTAGGCAACGTGCTATAGCCATTAAGTACAGAGAGGTACCAGGAAATTATTATGCTTATATTTTCATGCGTAACTTTTTTTATAGTGCTGTGACTTTCTGCACCACAGGGACATTTTCCATATTAGAAAGAAAATGATGTCTAGGCTTTGCTTCTTTTTGCATTCATAGCTCTTAAACTATTCCTTTAACTTATTTTCTGTTTCATATTGTTGTGAGCTTTCAGTATTTTCTGCAGTATCTGATGTTTAAATTCATTCTTGCTAGGTGCTGTGAAAGTCTTTTAGGTGTTCTTTTACACGTACATTATGGGGGGAAAATTCAATTTTTACCCCTAAACTTGGCTCAATGAATCAGTTTAAACTTAAGCTTTCAATTTTTTAAGTTCTGTGGTTAGTTATGAGTATTCTTGTGGCTTGGTTGAAATCAAGCATGAGCTTTAATACGCTATTATATCATATGTTTATGCATTTAAACTGGATGTTTGTTTTTTGTTTTTTTTTCTTTTGGGGGGGGGGGGGGGGGGGTTTAACTTTTTGGTTTTTCCTTTGAGAATTTGCGGTGGGTGGAAAATAAGAATTATATAATTGCAAATATAGCACAATGTAAAAAAAAATTGCAAATATAGCAAAATCTATCAGAGTATATTACTGATAGACACTGATAGACTCTAGTTAATTAGAATCTATCTGATAGACTTCGATTATCAGAGTTTATCAACGATAGACTCTGATTAATCAGAATCTATCGCTGATAGACTCCGGTTAATCGAAATTTATCAGTGACTATCATTGATAGACTTTGATTAATCAAATTCTATCAGTGATAGTCACTGATAGATTTTGATTAATTGGAGTCTATCAGTGATAGACACTGATAGACTTTGAATAATCAGAGTCTATCGGTGATAGACACAGATAGAATCTGACGACAACTTTTTGGTCTTGTACAATTAATTTTGCTATATATGCAATTAGTTTGCAACTGTGCTATATTTGCTATTAATTTGGTCCAATTTTCCATCTATGCAACTGCCCCTTTTCCTTTTGGATATGTGCATCATCTTTTTCTTATCCCAAAAAACACATCTCCTTTTCATTGTTTTAAAAACATTCTGCTGTTCCAGGGCCTGCAGAAGATCTTTGATGAACAGAAGATGAAGCTCTGGGAAGAGTTATATGAAATGACTGATGTCTATACAGAGAGATGGTTCTCCTCAGGTATTCTTATAATTATCATGCAGCTTTGAGCCTTTTGATGATTCGGATGTGTGGTATACCCTTAAATACTCAAACGATTCGAGTATTTCATTTCCTTTTATAGAGGAGATAGATAATGTTATATAAAAGGATTATAATTGTAGTAGACTAAATAATCCCAAATTTTGGGATTTTGCATCGGAAATAAGCACTTGAATTTCTTGCCACTTTGCTATGTTAACTCTGATGATCATTGAGATCGGAAGTCTGGCTGTCTGAGTCTGATTGTTGAGGAAATCTCGCATAGTAGTTAACAATTGGGTGTAATCGTGCGTACTAAACTACATTTGATATTTGGATAATATTTTTAATAGTCAACTACCTATCCTTATAACCCTTAGGCCTTATCATTGTTGACTACAATATGTATGCTATAGTGTACTTAATCCTCTAGCTAAAGTTTTTGTTGCTTCTCTAAAAACTTGCTAATGATCAATCTGTTATATGCCATTCTAGCACGAGCTTTCTTGGAGGAGTGTTCGTTTTCAGATAAACCACCACTTGCCTCTGCTGATCGGACATCAAACACTACTTCTAGAAGTTCCCAGCATGTTAATGTCTTGGTGACTTCTGGATCATTGATACCCAGCCTCGTGAAATGCTTACTATTTCGGCTCGATAATTTGATAACACCTGGAAATGGTGAGTCATTCCCTTTTTTCTCCTTTTTGATGTTTCTTCAGCCGTATTTTCAGAGTCAATTTCCAGTCAGAAGCCTCTTTTCCCTGCAATTGGTGAACTTGTTTACAAGTTGTCTTAATAATTAACTTCAAATCGGTTACTTTTCCAGTCTACAGTTCGTGGGAAGTGGGAAAACTTCAATGTTTCCAGTGGATCAAGGAGCGTTTTAACAAGTCAAATGTTCGTTTCTGCGTAATAGGCGATGGATGGGAAGAGTGCGAAGCTGCACAGTCCATGAAATGGCCATTTGTTAAGATTGATCTGCAACCTGGAAGTCCTCACAGGTTTCCTGGTCTGTCTTTGAAAACACTAGGCTTTTACTTCTCTGTTATTTATGGTAACTCCGATTCATCAAGTGATGAAGAATAGTTCACATAATCAGCAACAGATAAGCAGTCATTTTCCAGGCTTTAACTCTGTATTCTTACGAATTACAACAGTTAAATATTATGGCTGTCATTTATGTTCAAAGGTTGTACGTAATCAAAATTATTAGCAGGCCATAATAAGGCTGTATTTTCCCTCCATTCTGTACCGTACCTGTAATCAGAGAATCCAGTAAAACTCACTGGTTTTTTCTACTGCTTGTATTGATCAATCTTCAGTGGCTGTAATCTTTATTCTGAGAATACGAAACTTGGTTATCTTAAGCTGCTTTTCGCATTTGGGATGAGCTAAATTTAAGTTTCAGG

mRNA sequence

ATGGAAGAAAGTAGAAATCATCATCTTTCTGAACAAAGTACAAAGGGGTTTGCTAAAAGCACCAAGGATCAGAAACTTAATGTGTATGTGTGGGACATGGATGAGACTATCATCCTGCTCAAGTCCTTGTTGAATGGGACATATGCTGAGGCTTTTTGTGGTTCAAAGGATGTTAAAAGGGGTGAAGAACTTGGAAAAATTTGGGAGAAGCAGATTCTTGATCTGTGTGATCATCACTTCTTCTATGAACAAATAGAGAACTATAATCAGCCCTTTCTTGATGCATTGAGTGAATATGATGATGGCCGTGATCTTTCTAATTATGATTTTGACCAAGATGGTTTTGGTCCACCATATGATGATGGAAACAAAAGAAAACTGGCTTTTAGGCAACGTGCTATAGCCATTAAGTACAGAGAGGGCCTGCAGAAGATCTTTGATGAACAGAAGATGAAGCTCTGGGAAGAGTTATATGAAATGACTGATGTCTATACAGAGAGATGGTTCTCCTCAGCACGAGCTTTCTTGGAGGAGTGTTCGTTTTCAGATAAACCACCACTTGCCTCTGCTGATCGGACATCAAACACTACTTCTAGAAGTTCCCAGCATGTTAATGTCTTGGTGACTTCTGGATCATTGATACCCAGCCTCGTGAAATGCTTACTATTTCGGCTCGATAATTTGATAACACCTGGAAATGTCTACAGTTCGTGGGAAGTGGGAAAACTTCAATGTTTCCAGTGGATCAAGGAGCGTTTTAACAAGTCAAATGTTCGTTTCTGCGTAATAGGCGATGGATGGGAAGAGTGCGAAGCTGCACAGTCCATGAAATGGCCATTTGTTAAGATTGATCTGCAACCTGGAAGTCCTCACAGGTTTCCTGGTCTGTCTTTGAAAACACTAGGCTTTTACTTCTCTGTTATTTATGGTAACTCCGATTCATCAAGTGATGAAGAATAG

Coding sequence (CDS)

ATGGAAGAAAGTAGAAATCATCATCTTTCTGAACAAAGTACAAAGGGGTTTGCTAAAAGCACCAAGGATCAGAAACTTAATGTGTATGTGTGGGACATGGATGAGACTATCATCCTGCTCAAGTCCTTGTTGAATGGGACATATGCTGAGGCTTTTTGTGGTTCAAAGGATGTTAAAAGGGGTGAAGAACTTGGAAAAATTTGGGAGAAGCAGATTCTTGATCTGTGTGATCATCACTTCTTCTATGAACAAATAGAGAACTATAATCAGCCCTTTCTTGATGCATTGAGTGAATATGATGATGGCCGTGATCTTTCTAATTATGATTTTGACCAAGATGGTTTTGGTCCACCATATGATGATGGAAACAAAAGAAAACTGGCTTTTAGGCAACGTGCTATAGCCATTAAGTACAGAGAGGGCCTGCAGAAGATCTTTGATGAACAGAAGATGAAGCTCTGGGAAGAGTTATATGAAATGACTGATGTCTATACAGAGAGATGGTTCTCCTCAGCACGAGCTTTCTTGGAGGAGTGTTCGTTTTCAGATAAACCACCACTTGCCTCTGCTGATCGGACATCAAACACTACTTCTAGAAGTTCCCAGCATGTTAATGTCTTGGTGACTTCTGGATCATTGATACCCAGCCTCGTGAAATGCTTACTATTTCGGCTCGATAATTTGATAACACCTGGAAATGTCTACAGTTCGTGGGAAGTGGGAAAACTTCAATGTTTCCAGTGGATCAAGGAGCGTTTTAACAAGTCAAATGTTCGTTTCTGCGTAATAGGCGATGGATGGGAAGAGTGCGAAGCTGCACAGTCCATGAAATGGCCATTTGTTAAGATTGATCTGCAACCTGGAAGTCCTCACAGGTTTCCTGGTCTGTCTTTGAAAACACTAGGCTTTTACTTCTCTGTTATTTATGGTAACTCCGATTCATCAAGTGATGAAGAATAG

Protein sequence

MEESRNHHLSEQSTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDVKRGEELGKIWEKQILDLCDHHFFYEQIENYNQPFLDALSEYDDGRDLSNYDFDQDGFGPPYDDGNKRKLAFRQRAIAIKYREGLQKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEECSFSDKPPLASADRTSNTTSRSSQHVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSWEVGKLQCFQWIKERFNKSNVRFCVIGDGWEECEAAQSMKWPFVKIDLQPGSPHRFPGLSLKTLGFYFSVIYGNSDSSSDEE
Homology
BLAST of Spg021321 vs. NCBI nr
Match: XP_038903353.1 (eyes absent homolog [Benincasa hispida] >XP_038903354.1 eyes absent homolog [Benincasa hispida])

HSP 1 Score: 616.7 bits (1589), Expect = 1.1e-172
Identity = 295/319 (92.48%), Postives = 311/319 (97.49%), Query Frame = 0

Query: 1   MEESRNHHLSEQSTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDVKR 60
           ME S+N HLSEQSTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDVK+
Sbjct: 1   MEVSKN-HLSEQSTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDVKK 60

Query: 61  GEELGKIWEKQILDLCDHHFFYEQIENYNQPFLDALSEYDDGRDLSNYDFDQDGFGPPYD 120
           GEELGKIWEKQILDLCDHHFFYEQIENYNQPFL+ALSEYDDGRDLSNYDFDQD FGPP D
Sbjct: 61  GEELGKIWEKQILDLCDHHFFYEQIENYNQPFLNALSEYDDGRDLSNYDFDQDAFGPPCD 120

Query: 121 DGNKRKLAFRQRAIAIKYREGLQKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEECS 180
           DGNKRKLAFRQRAIA KYREGLQ IFD++KMKLWEELYEMTDVYT+RWFSSARAFLEECS
Sbjct: 121 DGNKRKLAFRQRAIANKYREGLQSIFDQEKMKLWEELYEMTDVYTDRWFSSARAFLEECS 180

Query: 181 FSDKPPLASADRTSNTTSRSSQHVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSWEV 240
           FSDKPPLAS+D+T N+TSRSSQHVNVLVTSG+LIPSLVKCLLFRLD+LITPGN+YSSWEV
Sbjct: 181 FSDKPPLASSDQTCNSTSRSSQHVNVLVTSGALIPSLVKCLLFRLDHLITPGNIYSSWEV 240

Query: 241 GKLQCFQWIKERFNKSNVRFCVIGDGWEECEAAQSMKWPFVKIDLQPGSPHRFPGLSLKT 300
           GK+QCFQWIKERF+KSNVRFC IGDGWEECEAAQS+KWPF+KIDLQPGSPHRFPGLSLKT
Sbjct: 241 GKVQCFQWIKERFDKSNVRFCAIGDGWEECEAAQSLKWPFIKIDLQPGSPHRFPGLSLKT 300

Query: 301 LGFYFSVIYGNSDSSSDEE 320
           LGFYFSVIYGNSDSSSDEE
Sbjct: 301 LGFYFSVIYGNSDSSSDEE 318

BLAST of Spg021321 vs. NCBI nr
Match: KAG6577695.1 (Eyes absent-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 615.5 bits (1586), Expect = 2.5e-172
Identity = 292/319 (91.54%), Postives = 310/319 (97.18%), Query Frame = 0

Query: 1   MEESRNHHLSEQSTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDVKR 60
           ME+S+N H SE++TKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKD+KR
Sbjct: 1   MEDSKN-HFSEETTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDIKR 60

Query: 61  GEELGKIWEKQILDLCDHHFFYEQIENYNQPFLDALSEYDDGRDLSNYDFDQDGFGPPYD 120
           GEELGKIWEKQILDLCDHHFFYEQIENYNQPF+DALSEYDDGRDLS+YDFDQDGF PPYD
Sbjct: 61  GEELGKIWEKQILDLCDHHFFYEQIENYNQPFIDALSEYDDGRDLSDYDFDQDGFDPPYD 120

Query: 121 DGNKRKLAFRQRAIAIKYREGLQKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEECS 180
           DGNKRKLAFRQRAIA KY+EGLQ IFDEQKMK WEELYEMTDVYT+RWFSSARAFLEEC+
Sbjct: 121 DGNKRKLAFRQRAIANKYKEGLQNIFDEQKMKFWEELYEMTDVYTDRWFSSARAFLEECA 180

Query: 181 FSDKPPLASADRTSNTTSRSSQHVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSWEV 240
           FSD+PPL SADR  N+TSRSSQHVN+LVTSGSLIPSLVKCLLFRLD+LITPGNVYSSWEV
Sbjct: 181 FSDEPPLVSADRARNSTSRSSQHVNILVTSGSLIPSLVKCLLFRLDHLITPGNVYSSWEV 240

Query: 241 GKLQCFQWIKERFNKSNVRFCVIGDGWEECEAAQSMKWPFVKIDLQPGSPHRFPGLSLKT 300
           GKLQCF+WIKERFNK+NVRFCVIGDGWEECEAAQS+KWPFVKI+LQPGSPHRFPGLSLKT
Sbjct: 241 GKLQCFKWIKERFNKTNVRFCVIGDGWEECEAAQSLKWPFVKIELQPGSPHRFPGLSLKT 300

Query: 301 LGFYFSVIYGNSDSSSDEE 320
           LGFYFSVIYGNSDSSSDEE
Sbjct: 301 LGFYFSVIYGNSDSSSDEE 318

BLAST of Spg021321 vs. NCBI nr
Match: XP_022923383.1 (eyes absent homolog 2 [Cucurbita moschata] >XP_022923384.1 eyes absent homolog 2 [Cucurbita moschata])

HSP 1 Score: 614.8 bits (1584), Expect = 4.3e-172
Identity = 292/319 (91.54%), Postives = 310/319 (97.18%), Query Frame = 0

Query: 1   MEESRNHHLSEQSTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDVKR 60
           ME+S+NH  SE++TKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKD+KR
Sbjct: 1   MEDSKNHS-SEETTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDIKR 60

Query: 61  GEELGKIWEKQILDLCDHHFFYEQIENYNQPFLDALSEYDDGRDLSNYDFDQDGFGPPYD 120
           GEELGKIWEKQILDLCDHHFFYEQIENYNQPF+DALSEYDDGRDLS+YDFDQDGF PPYD
Sbjct: 61  GEELGKIWEKQILDLCDHHFFYEQIENYNQPFIDALSEYDDGRDLSDYDFDQDGFDPPYD 120

Query: 121 DGNKRKLAFRQRAIAIKYREGLQKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEECS 180
           DGNKRKLAFRQRAIA KY+EGLQ IFDEQKMK WEELYEMTDVYT+RWFSSARAFLEEC+
Sbjct: 121 DGNKRKLAFRQRAIANKYKEGLQNIFDEQKMKFWEELYEMTDVYTDRWFSSARAFLEECA 180

Query: 181 FSDKPPLASADRTSNTTSRSSQHVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSWEV 240
           FSD+PPL SADR  N+TSRSSQHVN+LVTSGSLIPSLVKCLLFRLD+LITPGNVYSSWEV
Sbjct: 181 FSDEPPLVSADRARNSTSRSSQHVNILVTSGSLIPSLVKCLLFRLDHLITPGNVYSSWEV 240

Query: 241 GKLQCFQWIKERFNKSNVRFCVIGDGWEECEAAQSMKWPFVKIDLQPGSPHRFPGLSLKT 300
           GKLQCF+WIKERFNK+NVRFCVIGDGWEECEAAQS+KWPFVKI+LQPGSPHRFPGLSLKT
Sbjct: 241 GKLQCFKWIKERFNKTNVRFCVIGDGWEECEAAQSLKWPFVKIELQPGSPHRFPGLSLKT 300

Query: 301 LGFYFSVIYGNSDSSSDEE 320
           LGFYFSVIYGNSDSSSDEE
Sbjct: 301 LGFYFSVIYGNSDSSSDEE 318

BLAST of Spg021321 vs. NCBI nr
Match: XP_022965107.1 (eyes absent homolog 4 [Cucurbita maxima] >XP_022965108.1 eyes absent homolog 4 [Cucurbita maxima])

HSP 1 Score: 614.4 bits (1583), Expect = 5.6e-172
Identity = 291/319 (91.22%), Postives = 310/319 (97.18%), Query Frame = 0

Query: 1   MEESRNHHLSEQSTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDVKR 60
           ME+S+NH  SE++TKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKD+KR
Sbjct: 1   MEDSKNHS-SEETTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDIKR 60

Query: 61  GEELGKIWEKQILDLCDHHFFYEQIENYNQPFLDALSEYDDGRDLSNYDFDQDGFGPPYD 120
           GEELGKIWE QILDLCDHHFFYEQIENYNQPF+DALSEYDDGRDLSNYDFDQDGF PPYD
Sbjct: 61  GEELGKIWETQILDLCDHHFFYEQIENYNQPFIDALSEYDDGRDLSNYDFDQDGFDPPYD 120

Query: 121 DGNKRKLAFRQRAIAIKYREGLQKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEECS 180
           DGNKRKLAFRQRAIA KY+EGLQ IFDEQK+K WEELYEMTDVYT+RWFSSARAFLEEC+
Sbjct: 121 DGNKRKLAFRQRAIANKYKEGLQNIFDEQKLKFWEELYEMTDVYTDRWFSSARAFLEECA 180

Query: 181 FSDKPPLASADRTSNTTSRSSQHVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSWEV 240
           FSD+PPL SADRT N+TSRSSQHVN+LVTSGSLIPSLVKCLLFRLD+LITPGNVYSSWEV
Sbjct: 181 FSDEPPLVSADRTRNSTSRSSQHVNILVTSGSLIPSLVKCLLFRLDHLITPGNVYSSWEV 240

Query: 241 GKLQCFQWIKERFNKSNVRFCVIGDGWEECEAAQSMKWPFVKIDLQPGSPHRFPGLSLKT 300
           GKLQCF+WIKERFNK+NVRFCV+GDGWEECEAAQS+KWPFVKI+LQPGSPHRFPGLSLKT
Sbjct: 241 GKLQCFKWIKERFNKTNVRFCVLGDGWEECEAAQSLKWPFVKIELQPGSPHRFPGLSLKT 300

Query: 301 LGFYFSVIYGNSDSSSDEE 320
           LGFYFSVIYGNSDSSSDEE
Sbjct: 301 LGFYFSVIYGNSDSSSDEE 318

BLAST of Spg021321 vs. NCBI nr
Match: XP_023553165.1 (eyes absent homolog 2 [Cucurbita pepo subsp. pepo] >XP_023553166.1 eyes absent homolog 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 610.9 bits (1574), Expect = 6.2e-171
Identity = 290/319 (90.91%), Postives = 310/319 (97.18%), Query Frame = 0

Query: 1   MEESRNHHLSEQSTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDVKR 60
           ME+S+N H SE++TKGFAKS+KDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKD+KR
Sbjct: 1   MEDSKN-HFSEETTKGFAKSSKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDIKR 60

Query: 61  GEELGKIWEKQILDLCDHHFFYEQIENYNQPFLDALSEYDDGRDLSNYDFDQDGFGPPYD 120
           GEELGKIWEKQILDLCDHHFFYEQIENYNQPF+DALSEYDDGRDLS+YDFDQDGF PPYD
Sbjct: 61  GEELGKIWEKQILDLCDHHFFYEQIENYNQPFIDALSEYDDGRDLSDYDFDQDGFDPPYD 120

Query: 121 DGNKRKLAFRQRAIAIKYREGLQKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEECS 180
           DGNKRKLAFRQRAIA KY+EGLQ IFDEQKMK W+ELYEMTDVYT+RWFSSARAFLEEC+
Sbjct: 121 DGNKRKLAFRQRAIANKYKEGLQNIFDEQKMKFWDELYEMTDVYTDRWFSSARAFLEECA 180

Query: 181 FSDKPPLASADRTSNTTSRSSQHVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSWEV 240
           FSD+PP ASADRT N+TSRSSQHVN+LVTSGSLIPSLVKCLLFRLD+LITP NVYSSWEV
Sbjct: 181 FSDEPPRASADRTRNSTSRSSQHVNILVTSGSLIPSLVKCLLFRLDHLITPENVYSSWEV 240

Query: 241 GKLQCFQWIKERFNKSNVRFCVIGDGWEECEAAQSMKWPFVKIDLQPGSPHRFPGLSLKT 300
           GKLQCF+WIKERFNK+NVRFCVIGDGWEECEAAQS+KWPFVKI+LQPGSPHRFPGLSLKT
Sbjct: 241 GKLQCFKWIKERFNKTNVRFCVIGDGWEECEAAQSLKWPFVKIELQPGSPHRFPGLSLKT 300

Query: 301 LGFYFSVIYGNSDSSSDEE 320
           LGFYFSVIYGNSDSSSDEE
Sbjct: 301 LGFYFSVIYGNSDSSSDEE 318

BLAST of Spg021321 vs. ExPASy Swiss-Prot
Match: O82162 (Eyes absent homolog OS=Arabidopsis thaliana OX=3702 GN=EYA PE=1 SV=2)

HSP 1 Score: 396.0 bits (1016), Expect = 4.1e-109
Identity = 189/297 (63.64%), Postives = 233/297 (78.45%), Query Frame = 0

Query: 23  DQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDVKRGEELGKIWEKQILDLCDHHFFY 82
           D  +NVYVWDMDET+ILL+SLLNGTYAE+F GSKDVKRG E+G++WEK IL +CD  FFY
Sbjct: 16  DGPVNVYVWDMDETLILLRSLLNGTYAESFNGSKDVKRGVEIGRMWEKHILKICDDFFFY 75

Query: 83  EQIENYNQPFLDALSEYDDGRDLSNYDFDQDGFGPPYDDGNKRKLAFRQRAIAIKYREGL 142
           EQ+E  N+PFLD+L +YDDG+DLS Y+F QD F  P DD NKRKLA+R RA+A +Y +GL
Sbjct: 76  EQVEECNEPFLDSLRQYDDGKDLSRYEFKQDDFSTPTDDLNKRKLAYRHRAVAERYEKGL 135

Query: 143 QKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEECSFSDKPPLASADRTSNTTSRSSQ 202
               D + M   +ELY +TD YT+RW SSARAFLE+CS  ++    S+D TS    +SSQ
Sbjct: 136 CPFIDSESMSGLDELYNVTDEYTDRWLSSARAFLEQCSCVEE----SSDGTS-AIEQSSQ 195

Query: 203 HVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSWEVGKLQCFQWIKERFNKSNVRFCV 262
            +++LVTSG+LIPSLVKCLLFRLD  +   NVYSS +VGKLQCF+WIKERFN    RFC 
Sbjct: 196 DIHILVTSGALIPSLVKCLLFRLDTFLRHENVYSSIDVGKLQCFKWIKERFNHPKFRFCA 255

Query: 263 IGDGWEECEAAQSMKWPFVKIDLQPGSPHRFPGLSLKTLGFYFSVIYGNSDSSSDEE 320
           IGDGWEEC AAQ+++WPFVKIDLQP S HRFPGL+ KT+ +YF+ +YGNSD+ S +E
Sbjct: 256 IGDGWEECAAAQALQWPFVKIDLQPDSSHRFPGLTPKTVSYYFAAVYGNSDADSSKE 307

BLAST of Spg021321 vs. ExPASy Swiss-Prot
Match: O95677 (Eyes absent homolog 4 OS=Homo sapiens OX=9606 GN=EYA4 PE=1 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 9.1e-32
Identity = 93/272 (34.19%), Postives = 134/272 (49.26%), Query Frame = 0

Query: 28  VYVWDMDETIILLKSLLNGTYAEAFCGSKDVKRGEELGKIWEKQILDLCDHHFFYEQIEN 87
           V+VWD+DETII+  SLL G+YA+ +   KD      LG   E+ I +L D H F+  +E 
Sbjct: 371 VFVWDLDETIIVFHSLLTGSYAQKY--GKDPPMAVTLGLRMEEMIFNLADTHLFFNDLEE 430

Query: 88  YNQPFLDALSEYDDGRDLSNYDFDQDGFGPPYDDGN-------------KRKLAFRQRAI 147
            +Q  +D +S  D+G+DLS Y F  DGF       N              RKLAFR R +
Sbjct: 431 CDQVHIDDVSSDDNGQDLSTYSFATDGFHAAASSANLCLPTGVRGGVDWMRKLAFRYRRV 490

Query: 148 A---IKYREGLQKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEECSFSDKPPLASAD 207
                 Y+  +  +    K   W +L    +  T+ W                  L +A 
Sbjct: 491 KELYNTYKNNVGGLLGPAKRDAWLQLRAEIEGLTDSW------------------LTNAL 550

Query: 208 RTSNTTSRSSQHVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSWEVGKLQCFQWIKE 267
           ++ +  S  S  +NVLVT+  LIP+L K LL+ L       N+YS+ ++GK  CF+ I +
Sbjct: 551 KSLSIISTRSNCINVLVTTTQLIPALAKVLLYSLGGAFPIENIYSATKIGKESCFERIMQ 610

Query: 268 RFNKSNVRFCVIGDGWEECEAAQSMKWPFVKI 284
           RF +  V + VIGDG EE +AA+    PF +I
Sbjct: 611 RFGR-KVVYVVIGDGVEEEQAAKKHNMPFWRI 621

BLAST of Spg021321 vs. ExPASy Swiss-Prot
Match: O00167 (Eyes absent homolog 2 OS=Homo sapiens OX=9606 GN=EYA2 PE=1 SV=2)

HSP 1 Score: 138.7 bits (348), Expect = 1.2e-31
Identity = 96/305 (31.48%), Postives = 146/305 (47.87%), Query Frame = 0

Query: 2   EESRNHHLSEQSTKGFAKSTKD-------QKLNVYVWDMDETIILLKSLLNGTYAEAFCG 61
           +  R H  S+   +G +K + D       +   V+VWD+DETII+  SLL GT+A  +  
Sbjct: 237 DTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRY-- 296

Query: 62  SKDVKRGEELGKIWEKQILDLCDHHFFYEQIENYNQPFLDALSEYDDGRDLSNYDFDQDG 121
            KD      +G + E+ I +L D H F+  +E+ +Q  +D +S  D+G+DLS Y+F  DG
Sbjct: 297 GKDTTTSVRIGLMMEEMIFNLADTHLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSADG 356

Query: 122 FGPPYDDGN-------------KRKLAFRQRAIA---IKYREGLQKIFDEQKMKLWEELY 181
           F       N              RKLAFR R +      Y+  +  +    K + W +L 
Sbjct: 357 FHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGLIGTPKRETWLQLR 416

Query: 182 EMTDVYTERWFSSARAFLEECSFSDKPPLASADRTSNTTSRSSQHVNVLVTSGSLIPSLV 241
              +  T+ W                  L  + +  N  +     VNVLVT+  LIP+L 
Sbjct: 417 AELEALTDLW------------------LTHSLKALNLINSRPNCVNVLVTTTQLIPALA 476

Query: 242 KCLLFRLDNLITPGNVYSSWEVGKLQCFQWIKERFNKSNVRFCVIGDGWEECEAAQSMKW 284
           K LL+ L ++    N+YS+ + GK  CF+ I +RF +  V + VIGDG EE + A+    
Sbjct: 477 KVLLYGLGSVFPIENIYSATKTGKESCFERIMQRFGRKAV-YVVIGDGVEEEQGAKKHNM 520

BLAST of Spg021321 vs. ExPASy Swiss-Prot
Match: Q9Z191 (Eyes absent homolog 4 OS=Mus musculus OX=10090 GN=Eya4 PE=2 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 1.5e-31
Identity = 93/272 (34.19%), Postives = 132/272 (48.53%), Query Frame = 0

Query: 28  VYVWDMDETIILLKSLLNGTYAEAFCGSKDVKRGEELGKIWEKQILDLCDHHFFYEQIEN 87
           V+VWD+DETII+  SLL G+YA+ +   KD      LG   E+ I +L D H F+  +E 
Sbjct: 348 VFVWDLDETIIVFHSLLTGSYAQKY--GKDPPMAVTLGLRMEEMIFNLADTHLFFNDLEE 407

Query: 88  YNQPFLDALSEYDDGRDLSNYDFDQDGFGPPYDDGN-------------KRKLAFRQRAI 147
            +Q  +D +S  D+G+DLS Y F  DGF       N              RKLAFR R +
Sbjct: 408 CDQVHIDDVSSDDNGQDLSTYSFATDGFHAAASSANLCLPTGVRGGVDWMRKLAFRYRRV 467

Query: 148 A---IKYREGLQKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEECSFSDKPPLASAD 207
                 Y+  +  +    K   W +L    +  T+ W                  L +A 
Sbjct: 468 KELYNTYKNNVGGLLGPAKRDAWLQLRAEIEGLTDSW------------------LTNAL 527

Query: 208 RTSNTTSRSSQHVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSWEVGKLQCFQWIKE 267
           ++ +  S  S  VNVLVT+  LIP+L K LL+ L       N+YS+ ++GK  CF+ I  
Sbjct: 528 KSLSIISTRSNCVNVLVTTTQLIPALAKVLLYSLGGAFPIENIYSATKIGKESCFERIVS 587

Query: 268 RFNKSNVRFCVIGDGWEECEAAQSMKWPFVKI 284
           RF  +N+ + VIGDG +E  AA     PF +I
Sbjct: 588 RFG-TNITYVVIGDGRDEEHAANQHNMPFWRI 598

BLAST of Spg021321 vs. ExPASy Swiss-Prot
Match: Q58DB6 (Eyes absent homolog 2 OS=Bos taurus OX=9913 GN=EYA2 PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.6e-31
Identity = 90/272 (33.09%), Postives = 134/272 (49.26%), Query Frame = 0

Query: 28  VYVWDMDETIILLKSLLNGTYAEAFCGSKDVKRGEELGKIWEKQILDLCDHHFFYEQIEN 87
           V+VWD+DETII+  SLL GT+A  +   KD      +G + E+ I +L D H F+  +E+
Sbjct: 269 VFVWDLDETIIIFHSLLTGTFASRY--GKDTTASVRIGLMMEEMIFNLADTHLFFNDLED 328

Query: 88  YNQPFLDALSEYDDGRDLSNYDFDQDGFGPPYDDGN-------------KRKLAFRQRAI 147
            +Q  +D +S  D+G+DLS Y+F  DGF       N              RKLAFR R +
Sbjct: 329 CDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRV 388

Query: 148 A---IKYREGLQKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEECSFSDKPPLASAD 207
                 Y+  +  +    K + W +L    +  T+ W                  L  + 
Sbjct: 389 KEMYNTYKNNVGGLIGAPKRETWLQLRAELEALTDLW------------------LTHSL 448

Query: 208 RTSNTTSRSSQHVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSWEVGKLQCFQWIKE 267
           +  N  +     VNVLVT+  LIP+L K LL+ L ++    N+YS+ + GK  CF+ I +
Sbjct: 449 KALNLINSRPNCVNVLVTTTQLIPALAKVLLYGLGSVFPIENIYSATKTGKESCFERIMQ 508

Query: 268 RFNKSNVRFCVIGDGWEECEAAQSMKWPFVKI 284
           RF +  V + VIGDG EE + A+    PF +I
Sbjct: 509 RFGRKAV-YIVIGDGVEEEQGAKKHNMPFWRI 519

BLAST of Spg021321 vs. ExPASy TrEMBL
Match: A0A6J1E691 (Protein-tyrosine-phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111431097 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.1e-172
Identity = 292/319 (91.54%), Postives = 310/319 (97.18%), Query Frame = 0

Query: 1   MEESRNHHLSEQSTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDVKR 60
           ME+S+NH  SE++TKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKD+KR
Sbjct: 1   MEDSKNHS-SEETTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDIKR 60

Query: 61  GEELGKIWEKQILDLCDHHFFYEQIENYNQPFLDALSEYDDGRDLSNYDFDQDGFGPPYD 120
           GEELGKIWEKQILDLCDHHFFYEQIENYNQPF+DALSEYDDGRDLS+YDFDQDGF PPYD
Sbjct: 61  GEELGKIWEKQILDLCDHHFFYEQIENYNQPFIDALSEYDDGRDLSDYDFDQDGFDPPYD 120

Query: 121 DGNKRKLAFRQRAIAIKYREGLQKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEECS 180
           DGNKRKLAFRQRAIA KY+EGLQ IFDEQKMK WEELYEMTDVYT+RWFSSARAFLEEC+
Sbjct: 121 DGNKRKLAFRQRAIANKYKEGLQNIFDEQKMKFWEELYEMTDVYTDRWFSSARAFLEECA 180

Query: 181 FSDKPPLASADRTSNTTSRSSQHVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSWEV 240
           FSD+PPL SADR  N+TSRSSQHVN+LVTSGSLIPSLVKCLLFRLD+LITPGNVYSSWEV
Sbjct: 181 FSDEPPLVSADRARNSTSRSSQHVNILVTSGSLIPSLVKCLLFRLDHLITPGNVYSSWEV 240

Query: 241 GKLQCFQWIKERFNKSNVRFCVIGDGWEECEAAQSMKWPFVKIDLQPGSPHRFPGLSLKT 300
           GKLQCF+WIKERFNK+NVRFCVIGDGWEECEAAQS+KWPFVKI+LQPGSPHRFPGLSLKT
Sbjct: 241 GKLQCFKWIKERFNKTNVRFCVIGDGWEECEAAQSLKWPFVKIELQPGSPHRFPGLSLKT 300

Query: 301 LGFYFSVIYGNSDSSSDEE 320
           LGFYFSVIYGNSDSSSDEE
Sbjct: 301 LGFYFSVIYGNSDSSSDEE 318

BLAST of Spg021321 vs. ExPASy TrEMBL
Match: A0A6J1HJG0 (Protein-tyrosine-phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111465071 PE=3 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 2.7e-172
Identity = 291/319 (91.22%), Postives = 310/319 (97.18%), Query Frame = 0

Query: 1   MEESRNHHLSEQSTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDVKR 60
           ME+S+NH  SE++TKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKD+KR
Sbjct: 1   MEDSKNHS-SEETTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDIKR 60

Query: 61  GEELGKIWEKQILDLCDHHFFYEQIENYNQPFLDALSEYDDGRDLSNYDFDQDGFGPPYD 120
           GEELGKIWE QILDLCDHHFFYEQIENYNQPF+DALSEYDDGRDLSNYDFDQDGF PPYD
Sbjct: 61  GEELGKIWETQILDLCDHHFFYEQIENYNQPFIDALSEYDDGRDLSNYDFDQDGFDPPYD 120

Query: 121 DGNKRKLAFRQRAIAIKYREGLQKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEECS 180
           DGNKRKLAFRQRAIA KY+EGLQ IFDEQK+K WEELYEMTDVYT+RWFSSARAFLEEC+
Sbjct: 121 DGNKRKLAFRQRAIANKYKEGLQNIFDEQKLKFWEELYEMTDVYTDRWFSSARAFLEECA 180

Query: 181 FSDKPPLASADRTSNTTSRSSQHVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSWEV 240
           FSD+PPL SADRT N+TSRSSQHVN+LVTSGSLIPSLVKCLLFRLD+LITPGNVYSSWEV
Sbjct: 181 FSDEPPLVSADRTRNSTSRSSQHVNILVTSGSLIPSLVKCLLFRLDHLITPGNVYSSWEV 240

Query: 241 GKLQCFQWIKERFNKSNVRFCVIGDGWEECEAAQSMKWPFVKIDLQPGSPHRFPGLSLKT 300
           GKLQCF+WIKERFNK+NVRFCV+GDGWEECEAAQS+KWPFVKI+LQPGSPHRFPGLSLKT
Sbjct: 241 GKLQCFKWIKERFNKTNVRFCVLGDGWEECEAAQSLKWPFVKIELQPGSPHRFPGLSLKT 300

Query: 301 LGFYFSVIYGNSDSSSDEE 320
           LGFYFSVIYGNSDSSSDEE
Sbjct: 301 LGFYFSVIYGNSDSSSDEE 318

BLAST of Spg021321 vs. ExPASy TrEMBL
Match: A0A6J1CWC3 (Protein-tyrosine-phosphatase OS=Momordica charantia OX=3673 GN=LOC111014894 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.7e-166
Identity = 288/319 (90.28%), Postives = 306/319 (95.92%), Query Frame = 0

Query: 1   MEESRNHHLSEQSTKGFAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDVKR 60
           ME+SRN H+S+QS+KG AKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEA  GSKDVK+
Sbjct: 1   MEKSRN-HVSDQSSKGIAKSTKDQKLNVYVWDMDETIILLKSLLNGTYAEALGGSKDVKK 60

Query: 61  GEELGKIWEKQILDLCDHHFFYEQIENYNQPFLDALSEYDDGRDLSNYDFDQDGFGPPYD 120
           G+ELGK+WEKQILDLCDHHFFYEQIENYNQPFLDA+SEYDDGRDLSNYDFDQDGFGPPYD
Sbjct: 61  GKELGKMWEKQILDLCDHHFFYEQIENYNQPFLDAVSEYDDGRDLSNYDFDQDGFGPPYD 120

Query: 121 DGNKRKLAFRQRAIAIKYREGLQKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEECS 180
           DGNKRKLAFRQRAIA KYREGLQ IFD+QKMKLWEELYEMTDVYT+RWFSSARAFLE+C 
Sbjct: 121 DGNKRKLAFRQRAIANKYREGLQNIFDQQKMKLWEELYEMTDVYTDRWFSSARAFLEDCL 180

Query: 181 FSDKPPLASADRTSNTTSRSSQHVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSWEV 240
           FSDKPPLASAD T NT SRSSQHV+VLVTSGSLIPSLVKCLLFRL++LITP NVYSSWEV
Sbjct: 181 FSDKPPLASADGTCNTASRSSQHVHVLVTSGSLIPSLVKCLLFRLNDLITPENVYSSWEV 240

Query: 241 GKLQCFQWIKERFNKSNVRFCVIGDGWEECEAAQSMKWPFVKIDLQPGSPHRFPGLSLKT 300
           GKLQCFQWIKERF++ NVRFCVIGDG EECEAAQSMKWPFVKIDLQPGSPHRFPGLSLKT
Sbjct: 241 GKLQCFQWIKERFDRPNVRFCVIGDGSEECEAAQSMKWPFVKIDLQPGSPHRFPGLSLKT 300

Query: 301 LGFYFSVIYGNSDSSSDEE 320
           LG+YFSVIYGNSDS+SDEE
Sbjct: 301 LGYYFSVIYGNSDSTSDEE 318

BLAST of Spg021321 vs. ExPASy TrEMBL
Match: A0A1S3BKY6 (Protein-tyrosine-phosphatase OS=Cucumis melo OX=3656 GN=LOC103490796 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 6.3e-161
Identity = 281/321 (87.54%), Postives = 302/321 (94.08%), Query Frame = 0

Query: 1   MEESRNHHLSEQSTKGFAKSTKDQ--KLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDV 60
           MEES+N HLSEQSTKGFAKSTKDQ  KLNVYVWDMDETIILLKSLL+GTYA+AF GSKDV
Sbjct: 1   MEESKN-HLSEQSTKGFAKSTKDQKEKLNVYVWDMDETIILLKSLLDGTYAKAFGGSKDV 60

Query: 61  KRGEELGKIWEKQILDLCDHHFFYEQIENYNQPFLDALSEYDDGRDLSNYDFDQDGFGPP 120
           KRGEELGK+WEK+ILDLCDH FFYEQIENYNQPFLDAL+EYDDGRDLSNYDFDQDGFGPP
Sbjct: 61  KRGEELGKMWEKEILDLCDHFFFYEQIENYNQPFLDALNEYDDGRDLSNYDFDQDGFGPP 120

Query: 121 YDDGNKRKLAFRQRAIAIKYREGLQKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEE 180
            DD NKRKLAFRQRAI  KYREGL+ IFD+QKMK WEELYEMTDVYT+RWFSSARAFLEE
Sbjct: 121 CDDVNKRKLAFRQRAITNKYREGLRNIFDQQKMKHWEELYEMTDVYTDRWFSSARAFLEE 180

Query: 181 CSFSDKPPLASADRTSNTTSRSSQHVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSW 240
           CS SD+PP ASAD+TSN+ S SSQHVN+LVTSG+LIPSLVKCLLFRLD+LITPGNVYSSW
Sbjct: 181 CSISDEPPHASADQTSNSNSTSSQHVNILVTSGALIPSLVKCLLFRLDHLITPGNVYSSW 240

Query: 241 EVGKLQCFQWIKERFNKSNVRFCVIGDGWEECEAAQSMKWPFVKIDLQPGSPHRFPGLSL 300
           EV K+QCFQWIKERF+K NVRFC IG+GWEECEAAQS+KWPFVKIDLQPGS HRFPGLSL
Sbjct: 241 EVEKVQCFQWIKERFDKPNVRFCAIGNGWEECEAAQSLKWPFVKIDLQPGSLHRFPGLSL 300

Query: 301 KTLGFYFSVIYGNSDSSSDEE 320
           KT+GFYFSVIYGNSDSS+DEE
Sbjct: 301 KTIGFYFSVIYGNSDSSNDEE 320

BLAST of Spg021321 vs. ExPASy TrEMBL
Match: A0A5D3CJY6 (Protein-tyrosine-phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001720 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 6.3e-161
Identity = 281/321 (87.54%), Postives = 302/321 (94.08%), Query Frame = 0

Query: 1   MEESRNHHLSEQSTKGFAKSTKDQ--KLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDV 60
           MEES+N HLSEQSTKGFAKSTKDQ  KLNVYVWDMDETIILLKSLL+GTYA+AF GSKDV
Sbjct: 1   MEESKN-HLSEQSTKGFAKSTKDQKEKLNVYVWDMDETIILLKSLLDGTYAKAFGGSKDV 60

Query: 61  KRGEELGKIWEKQILDLCDHHFFYEQIENYNQPFLDALSEYDDGRDLSNYDFDQDGFGPP 120
           KRGEELGK+WEK+ILDLCDH FFYEQIENYNQPFLDAL+EYDDGRDLSNYDFDQDGFGPP
Sbjct: 61  KRGEELGKMWEKEILDLCDHFFFYEQIENYNQPFLDALNEYDDGRDLSNYDFDQDGFGPP 120

Query: 121 YDDGNKRKLAFRQRAIAIKYREGLQKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEE 180
            DD NKRKLAFRQRAI  KYREGL+ IFD+QKMK WEELYEMTDVYT+RWFSSARAFLEE
Sbjct: 121 CDDVNKRKLAFRQRAITNKYREGLRNIFDQQKMKHWEELYEMTDVYTDRWFSSARAFLEE 180

Query: 181 CSFSDKPPLASADRTSNTTSRSSQHVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSW 240
           CS SD+PP ASAD+TSN+ S SSQHVN+LVTSG+LIPSLVKCLLFRLD+LITPGNVYSSW
Sbjct: 181 CSISDEPPHASADQTSNSNSTSSQHVNILVTSGALIPSLVKCLLFRLDHLITPGNVYSSW 240

Query: 241 EVGKLQCFQWIKERFNKSNVRFCVIGDGWEECEAAQSMKWPFVKIDLQPGSPHRFPGLSL 300
           EV K+QCFQWIKERF+K NVRFC IG+GWEECEAAQS+KWPFVKIDLQPGS HRFPGLSL
Sbjct: 241 EVEKVQCFQWIKERFDKPNVRFCAIGNGWEECEAAQSLKWPFVKIDLQPGSLHRFPGLSL 300

Query: 301 KTLGFYFSVIYGNSDSSSDEE 320
           KT+GFYFSVIYGNSDSS+DEE
Sbjct: 301 KTIGFYFSVIYGNSDSSNDEE 320

BLAST of Spg021321 vs. TAIR 10
Match: AT2G35320.1 (EYES ABSENT homolog )

HSP 1 Score: 396.0 bits (1016), Expect = 2.9e-110
Identity = 189/297 (63.64%), Postives = 233/297 (78.45%), Query Frame = 0

Query: 23  DQKLNVYVWDMDETIILLKSLLNGTYAEAFCGSKDVKRGEELGKIWEKQILDLCDHHFFY 82
           D  +NVYVWDMDET+ILL+SLLNGTYAE+F GSKDVKRG E+G++WEK IL +CD  FFY
Sbjct: 16  DGPVNVYVWDMDETLILLRSLLNGTYAESFNGSKDVKRGVEIGRMWEKHILKICDDFFFY 75

Query: 83  EQIENYNQPFLDALSEYDDGRDLSNYDFDQDGFGPPYDDGNKRKLAFRQRAIAIKYREGL 142
           EQ+E  N+PFLD+L +YDDG+DLS Y+F QD F  P DD NKRKLA+R RA+A +Y +GL
Sbjct: 76  EQVEECNEPFLDSLRQYDDGKDLSRYEFKQDDFSTPTDDLNKRKLAYRHRAVAERYEKGL 135

Query: 143 QKIFDEQKMKLWEELYEMTDVYTERWFSSARAFLEECSFSDKPPLASADRTSNTTSRSSQ 202
               D + M   +ELY +TD YT+RW SSARAFLE+CS  ++    S+D TS    +SSQ
Sbjct: 136 CPFIDSESMSGLDELYNVTDEYTDRWLSSARAFLEQCSCVEE----SSDGTS-AIEQSSQ 195

Query: 203 HVNVLVTSGSLIPSLVKCLLFRLDNLITPGNVYSSWEVGKLQCFQWIKERFNKSNVRFCV 262
            +++LVTSG+LIPSLVKCLLFRLD  +   NVYSS +VGKLQCF+WIKERFN    RFC 
Sbjct: 196 DIHILVTSGALIPSLVKCLLFRLDTFLRHENVYSSIDVGKLQCFKWIKERFNHPKFRFCA 255

Query: 263 IGDGWEECEAAQSMKWPFVKIDLQPGSPHRFPGLSLKTLGFYFSVIYGNSDSSSDEE 320
           IGDGWEEC AAQ+++WPFVKIDLQP S HRFPGL+ KT+ +YF+ +YGNSD+ S +E
Sbjct: 256 IGDGWEECAAAQALQWPFVKIDLQPDSSHRFPGLTPKTVSYYFAAVYGNSDADSSKE 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903353.11.1e-17292.48eyes absent homolog [Benincasa hispida] >XP_038903354.1 eyes absent homolog [Ben... [more]
KAG6577695.12.5e-17291.54Eyes absent-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022923383.14.3e-17291.54eyes absent homolog 2 [Cucurbita moschata] >XP_022923384.1 eyes absent homolog 2... [more]
XP_022965107.15.6e-17291.22eyes absent homolog 4 [Cucurbita maxima] >XP_022965108.1 eyes absent homolog 4 [... [more]
XP_023553165.16.2e-17190.91eyes absent homolog 2 [Cucurbita pepo subsp. pepo] >XP_023553166.1 eyes absent h... [more]
Match NameE-valueIdentityDescription
O821624.1e-10963.64Eyes absent homolog OS=Arabidopsis thaliana OX=3702 GN=EYA PE=1 SV=2[more]
O956779.1e-3234.19Eyes absent homolog 4 OS=Homo sapiens OX=9606 GN=EYA4 PE=1 SV=2[more]
O001671.2e-3131.48Eyes absent homolog 2 OS=Homo sapiens OX=9606 GN=EYA2 PE=1 SV=2[more]
Q9Z1911.5e-3134.19Eyes absent homolog 4 OS=Mus musculus OX=10090 GN=Eya4 PE=2 SV=2[more]
Q58DB62.6e-3133.09Eyes absent homolog 2 OS=Bos taurus OX=9913 GN=EYA2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E6912.1e-17291.54Protein-tyrosine-phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111431097 PE=3 ... [more]
A0A6J1HJG02.7e-17291.22Protein-tyrosine-phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111465071 PE=3 SV... [more]
A0A6J1CWC31.7e-16690.28Protein-tyrosine-phosphatase OS=Momordica charantia OX=3673 GN=LOC111014894 PE=3... [more]
A0A1S3BKY66.3e-16187.54Protein-tyrosine-phosphatase OS=Cucumis melo OX=3656 GN=LOC103490796 PE=3 SV=1[more]
A0A5D3CJY66.3e-16187.54Protein-tyrosine-phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
Match NameE-valueIdentityDescription
AT2G35320.12.9e-11063.64EYES ABSENT homolog [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038102EYA domain superfamilyGENE3D3.40.50.12350coord: 27..289
e-value: 1.0E-90
score: 305.8
IPR006545EYA domainTIGRFAMTIGR01658TIGR01658coord: 26..301
e-value: 4.5E-120
score: 398.5
NoneNo IPR availableSFLDSFLDG01129C1.5:_HAD,_Beta-PGM,_Phosphatcoord: 26..284
e-value: 1.2E-13
score: 46.0
NoneNo IPR availableSFLDSFLDS00003Haloacid_Dehalogenasecoord: 26..284
e-value: 1.2E-13
score: 46.0
NoneNo IPR availablePANTHERPTHR10190:SF16DEVELOPMENTAL PROTEIN EYES ABSENTcoord: 10..310
IPR028472Eyes absent familyPANTHERPTHR10190EYES ABSENTcoord: 10..310
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 27..285

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg021321.1Spg021321.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030154 cell differentiation
biological_process GO:0016576 histone dephosphorylation
biological_process GO:0007275 multicellular organism development
biological_process GO:0035335 peptidyl-tyrosine dephosphorylation
biological_process GO:0045739 positive regulation of DNA repair
cellular_component GO:0005634 nucleus
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030946 protein tyrosine phosphatase activity, metal-dependent
molecular_function GO:0004725 protein tyrosine phosphatase activity