Spg021266 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg021266 vs. NCBI nr
Match: XP_031738649.1 (uncharacterized protein LOC116402744 [Cucumis sativus] >KAE8650030.1 hypothetical protein Csa_009857 [Cucumis sativus])

HSP 1 Score: 508.8 bits (1309), Expect = 4.6e-140
Identity = 259/437 (59.27%), Postives = 326/437 (74.60%), Query Frame = 0

Query: 3   SKATWLVIVLFVCFNCKCNQASNSNLSREEELELEAQLKLLNKPSIKTFQTKEGDIIDCV 62
           SKAT LVIV FVCFNCK N ASN NLSREE+LE+E QLKLLNKP IKT++TKEGDIIDCV
Sbjct: 6   SKATCLVIVFFVCFNCKFNHASNPNLSREEDLEIERQLKLLNKPFIKTYKTKEGDIIDCV 65

Query: 63  DINKQPALDHPLLKNHKVQTRPSRHLSNLFK-DSSESNDNSFASSIDR-EPCPVGFVPIR 122
           DINKQPALDHPLLKNHKVQT PS  +S LFK DSS+SN+    S+ +  E CPVGFVPIR
Sbjct: 66  DINKQPALDHPLLKNHKVQTLPSEFVSKLFKEDSSQSNNGILTSNNNNGEGCPVGFVPIR 125

Query: 123 RTLKKDLLRIRSLSSKKPKSRSDQSLNKLEDDYN-DYFYDIVKFPYDQNVVSHSMVKGGR 182
           RTLK+DL+R++SLSS     +S  S+N  +DD + D FYD V+FPY QNVVSHS++K   
Sbjct: 126 RTLKEDLIRLKSLSSNSKNQQS--SMNPEDDDLSGDSFYDAVRFPYYQNVVSHSLIKAQY 185

Query: 183 YYGADARFAVYDVNVTSD-QSSSSNIWVTGGPSDAALNVLLAGWQANPSINGDSFPRLFV 242
           Y+GA AR AV++V+++ + QSSS+NIWV GG SD +LNVL+AGWQ NP++NGD+ PR FV
Sbjct: 186 YHGAKARIAVHNVSLSDNGQSSSANIWVLGG-SDDSLNVLMAGWQVNPAVNGDNLPRTFV 245

Query: 243 YWTTDAGAATGCYNYLCQGFVQVHRSISAGARLSPSSTYQGRQYEFKFGIIQVINTQILN 302
           YWT D G  TGCYN LCQGFV V+ +I  G+ + P+S YQG+QY+++F I+Q I      
Sbjct: 246 YWTVDTGVTTGCYNMLCQGFVLVNPNIHVGSSILPASIYQGQQYDYQFSIVQAI------ 305

Query: 303 VDVDRNVEEESNNWWVLVGEKQEAMGYWPSELFPHLRNGSDQVAWGGIAKPSQDG-ESPP 362
                       +WWV VG+ Q  +GYWP+ELFP+L  G+DQVAWGG A+P+  G ESPP
Sbjct: 306 -----------GHWWVRVGDNQVGLGYWPNELFPNLLRGADQVAWGGSAQPTLYGDESPP 365

Query: 363 LGNGHKPNKGQYNEGCFIRSIHYLTEGSKVESPNWYNTLSAVSSTDCYGLNATNQNCGYD 422
           LG+GHKPN G+ +E  F+R+I Y+     +  P   NT++ VS++ CY L  +N+NC +D
Sbjct: 366 LGSGHKPN-GKPDEAIFVRNIQYIAPNYILSIPTLNNTINYVSNSSCYDL-ISNENCSFD 420

Query: 423 TFKYCFTYGGPGGHGCQ 435
            FKYCFT+GGPGGHGC+
Sbjct: 426 PFKYCFTFGGPGGHGCE 420

BLAST of Spg021266 vs. NCBI nr
Match: TYK11502.1 (neprosin 2 [Cucumis melo var. makuwa])

HSP 1 Score: 479.6 bits (1233), Expect = 3.0e-131
Identity = 244/428 (57.01%), Postives = 309/428 (72.20%), Query Frame = 0

Query: 11  VLFVCFNCKCNQASNSNLSREEELELEAQLKLLNKPSIKTFQTKEGDIIDCVDINKQPAL 70
           + FVCFNCK N ASN NLSREEELE+E QLKLLNKP IKT++TKEGDIIDCVDINKQPAL
Sbjct: 211 LFFVCFNCKFNHASNPNLSREEELEIERQLKLLNKPFIKTYKTKEGDIIDCVDINKQPAL 270

Query: 71  DHPLLKNHKVQTRPSRHLSNLFKDSSESNDNS---FASSIDREPCPVGFVPIRRTLKKDL 130
           DHPLLKNHKVQT PS  +S LFK+ S S  N+    +++ + E CP+GFVPIRRTLK+DL
Sbjct: 271 DHPLLKNHKVQTLPSEFISKLFKEDSISQSNNGILTSNNNNGEGCPIGFVPIRRTLKEDL 330

Query: 131 LRIRSLSSKKPKSRSDQSLNKLEDDYNDYFYDIVKFPYDQNVVSHSMVKGGRYYGADARF 190
           +R++SLSS   K  S  S+   +D   D+ +D V+FPYDQNVVSHS++K   Y+GA AR 
Sbjct: 331 IRLKSLSSNYKKQES--SMKPQDDQSKDFSHDAVRFPYDQNVVSHSLIKATYYHGAKARI 390

Query: 191 AVYDVNVTSD-QSSSSNIWVTGGPSDAALNVLLAGWQANPSINGDSFPRLFVYWTTDAGA 250
           AVY+V+++ + QSSS+NIWV GGP D +LNVL+A      +++GDS PR FVYWTTD GA
Sbjct: 391 AVYNVSLSDENQSSSANIWVVGGP-DESLNVLMA------AVSGDSLPRTFVYWTTDRGA 450

Query: 251 ATGCYNYLCQGFVQVHRSISAGARLSPSSTYQGRQYEFKFGIIQVINTQILNVDVDRNVE 310
            TGCYN LCQGFV V+  I  G+ + P+S YQG+QY+++F I+Q I              
Sbjct: 451 TTGCYNMLCQGFVLVNPDIPVGSSILPASIYQGKQYDYQFSIVQAI-------------- 510

Query: 311 EESNNWWVLVGEKQEAMGYWPSELFPHLRNGSDQVAWGGIAKPS-QDGESPPLGNGHKPN 370
               +WWV VG+ Q  +GYWPSELFP+L  G++QVAWGG A+PS    ESPPLG+GHKPN
Sbjct: 511 ---GHWWVRVGDDQVGLGYWPSELFPNLLRGAEQVAWGGSAEPSLYSDESPPLGSGHKPN 570

Query: 371 KGQYNEGCFIRSIHYLTEGSKVESPNWYNTLSAVSSTDCYGLNATNQNCGYDTFKYCFTY 430
            G+ +E CF+R+I Y+     +  P   NT++ VSS+ CY L  +N+NC +D FKYCFT+
Sbjct: 571 -GRPDEACFVRNIQYIASNYILSIPTLDNTINYVSSSSCYDL-ISNENCDFDPFKYCFTF 610

Query: 431 GGPGGHGC 434
           GGPGG  C
Sbjct: 631 GGPGGQDC 610

BLAST of Spg021266 vs. NCBI nr
Match: KAE8650029.1 (hypothetical protein Csa_011504 [Cucumis sativus])

HSP 1 Score: 463.0 bits (1190), Expect = 2.9e-126
Identity = 247/442 (55.88%), Postives = 305/442 (69.00%), Query Frame = 0

Query: 19  KCNQASNSNLSREEELELEAQLKLLNKPSIKT------------FQTKEGDIIDCVDINK 78
           K ++ASNS LSREEELE+E  LKLLNKPSIKT            +QTKEGDIIDCVDINK
Sbjct: 12  KISEASNSKLSREEELEIEEHLKLLNKPSIKTYKSTSRYTRACSYQTKEGDIIDCVDINK 71

Query: 79  QPALDHPLLKNHKVQTRPSRHLSNLF-KDSSESNDNSFA----SSIDREPCPVGFVPIRR 138
           QPALDHPLLKNHKVQT PS ++S LF KDSS++N+        ++   E CP GFVPIRR
Sbjct: 72  QPALDHPLLKNHKVQTLPSGYVSKLFKKDSSQANNGISTLPSNNNNGEEGCPNGFVPIRR 131

Query: 139 TLKKDLLRIRSLSSKKPKSRSDQSLNKLEDDYNDYFYDIVKFPYDQNVVSHSMVKG-GRY 198
           TLKKDL+R++SLSS     +S  S+N  +D  +D+F D VKFPY QNVVSHS+ KG  +Y
Sbjct: 132 TLKKDLIRLKSLSSNNKNQQS--SMNPQDDQSDDFFDDSVKFPYYQNVVSHSLEKGTEKY 191

Query: 199 YGADARFAVYDVNVTSDQSSSSNIWVTGGPSDAALNVLLAGWQANPSINGDSFPRLFVYW 258
           YG  +  +VY+V+++ DQSSS+NIW+ GGP D +L VL+ GW  NP +NGD   R FVYW
Sbjct: 192 YGTKSYMSVYNVSLSFDQSSSTNIWIVGGPVD-SLGVLMTGWLVNPEVNGDFVTRSFVYW 251

Query: 259 TTDAGAATGCYNYLCQGFVQVHRSISAGARLSPSSTYQGRQYEFKFGIIQVINTQILNVD 318
           T D G  TGCYN  CQGFVQV+ S   GA L P+STY+G+QY+++F IIQ+         
Sbjct: 252 TADGGTTTGCYNMYCQGFVQVNPSHHVGAPLLPTSTYEGQQYDYQFTIIQI--------- 311

Query: 319 VDRNVEEESNNWWVLVGEKQEAMGYWPSELFPHLRNGSDQVAWGGIAKPSQDGESPPLGN 378
                     NWWVLVGE    +GYWP EL  +L +G+DQ+AWGGIA+PS DG SP LG+
Sbjct: 312 --------EGNWWVLVGENL-GLGYWPKELIQNLVDGADQIAWGGIAQPSIDGVSPMLGS 371

Query: 379 GHKPNK-GQYNEGCFIRSIHYLTEGSKVES---PNWYNTLSAVSSTDCYGLNATNQNCGY 438
           GHKPN+ G YNEGC+IR+I  ++ G+   +   P W NTLS  S+T CY LN  N NCGY
Sbjct: 372 GHKPNENGDYNEGCYIRNIQIIS-GAATNTYVLPTWDNTLSYSSNTSCYDLN-PNVNCGY 430

BLAST of Spg021266 vs. NCBI nr
Match: XP_022145288.1 (uncharacterized protein LOC111014777 [Momordica charantia])

HSP 1 Score: 393.7 bits (1010), Expect = 2.1e-105
Identity = 213/434 (49.08%), Postives = 266/434 (61.29%), Query Frame = 0

Query: 1   MASKA-TWLVIVLFVCFNCKCNQASNSNLSREEELELEAQLKLLNKPSIKTFQTKEGDII 60
           MASKA  WL+IVL +  NCK + A +SNLSREEELELE QLKLLN+P I TFQT+EGDII
Sbjct: 1   MASKAIMWLMIVLLLHLNCKGSLAFDSNLSREEELELEMQLKLLNRPFITTFQTEEGDII 60

Query: 61  DCVDINKQPALDHPLLKNHKVQTRPSRHLSNLFKDSSESNDNSFASSIDREPCPVGFVPI 120
           DCVDINKQPALDHP LK+HK+QTRPS +   L KDSS S D SF ++ +R  CP G+VPI
Sbjct: 61  DCVDINKQPALDHPSLKSHKIQTRPSTYPFGLSKDSSSSRDKSFINNNNR-ACPAGYVPI 120

Query: 121 RRTLKKDLLRIRSLSSKKPKSRSDQSLNKLEDDYNDYFYDIVKFPYDQNVVSHSMVKGGR 180
           RRT+KKDL+RIRSLSSK+P                      V FPY+Q+VVS +M KG +
Sbjct: 121 RRTIKKDLIRIRSLSSKEPTGIKTSIKGG------------VDFPYNQDVVSVAMKKGIK 180

Query: 181 YYGADARFAVYDVNVTSDQSSSSNIWVTGGPSDAALNVLLAGWQANPSINGDSFPRLFVY 240
           YYGA    +VY+++V  DQSSSSNIW+ GGP  A  NV+LAGWQ NP INGDS  R+FVY
Sbjct: 181 YYGASGSVSVYNLSVAQDQSSSSNIWIIGGPPQAP-NVILAGWQVNPMINGDSLTRMFVY 240

Query: 241 WTTDAGAATGCYNYLCQGFVQVHRSISAGARLSPSSTYQGRQYEFKFGIIQVINTQILNV 300
           WT                                                          
Sbjct: 241 WT---------------------------------------------------------- 300

Query: 301 DVDRNVEEESNNWWVLVGEKQEAMGYWPSELFPHLRNGSDQVAWGGIAKPSQDGESPPLG 360
                 +  + NWW+ VGE  + +GYWP ELF HL +G++QVAWGGIAKPS +G SPPLG
Sbjct: 301 ------DRPTGNWWLAVGESHKTIGYWPKELFGHLNDGTEQVAWGGIAKPSPNGMSPPLG 355

Query: 361 NGHKPNKGQYNEGCFIRSIHYLTEGSKVESPNWYNTLSAVSSTDCYGLNATNQNCGYDTF 420
           NGHKPN  +Y++ C+ R ++Y+ E +K + P   NT + +S+  CY L+   + CG + F
Sbjct: 361 NGHKPNYSKYDDACYFRYMNYVDENNKGQFPANENTANYLSNNSCYALD-NRETCGGEFF 355

Query: 421 KYCFTYGGPGGHGC 434
            YC T+GGPGG+ C
Sbjct: 421 YYCITFGGPGGNNC 355

BLAST of Spg021266 vs. NCBI nr
Match: XP_031738648.1 (uncharacterized protein LOC105435061 [Cucumis sativus])

HSP 1 Score: 360.9 bits (925), Expect = 1.5e-95
Identity = 199/423 (47.04%), Postives = 243/423 (57.45%), Query Frame = 0

Query: 21  NQASNSNLSREEELELEAQLKLLNKPSIKTFQTKEGDIIDCVDINKQPALDHPLLKNHKV 80
           ++ASNS LSREEELE+E  LKLLNKPSIKT++TKEGDIIDCVDINKQPALDHPLLKNHKV
Sbjct: 30  SEASNSKLSREEELEIEEHLKLLNKPSIKTYKTKEGDIIDCVDINKQPALDHPLLKNHKV 89

Query: 81  QTRPSRHLSNLFKDSSESNDNSFASSIDREPCPVGFVPIRRTLKKDLLRIRSLSSKKPKS 140
           Q                                                           
Sbjct: 90  Q----------------------------------------------------------- 149

Query: 141 RSDQSLNKLEDDYNDYFYDIVKFPYDQNVVSHSMVKG-GRYYGADARFAVYDVNVTSDQS 200
                                       VVSHS+ KG  +YYG  +  +VY+V+++ DQS
Sbjct: 150 ----------------------------VVSHSLEKGTEKYYGTKSYMSVYNVSLSFDQS 209

Query: 201 SSSNIWVTGGPSDAALNVLLAGWQANPSINGDSFPRLFVYWTTDAGAATGCYNYLCQGFV 260
           SS+NIW+ GGP D +L VL+ GW  NP +NGD   R FVYWT D G  TGCYN  CQGFV
Sbjct: 210 SSTNIWIVGGPVD-SLGVLMTGWLVNPEVNGDFVTRSFVYWTADGGTTTGCYNMYCQGFV 269

Query: 261 QVHRSISAGARLSPSSTYQGRQYEFKFGIIQVINTQILNVDVDRNVEEESNNWWVLVGEK 320
           QV+ S   GA L P+STY+G+QY+++F IIQ+                   NWWVLVGE 
Sbjct: 270 QVNPSHHVGAPLLPTSTYEGQQYDYQFTIIQI-----------------EGNWWVLVGEN 329

Query: 321 QEAMGYWPSELFPHLRNGSDQVAWGGIAKPSQDGESPPLGNGHKPNK-GQYNEGCFIRSI 380
              +GYWP EL  +L +G+DQ+AWGGIA+PS DG SP LG+GHKPN+ G YNEGC+IR+I
Sbjct: 330 L-GLGYWPKELIQNLVDGADQIAWGGIAQPSIDGVSPMLGSGHKPNENGDYNEGCYIRNI 344

Query: 381 HYLTEGSKVES---PNWYNTLSAVSSTDCYGLNATNQNCGYDTFKYCFTYGGPGGHGCQL 439
             ++ G+   +   P W NTLS  S+T CY LN  N NCGYD  +YCFT+GGPGG  C+ 
Sbjct: 390 QIIS-GAATNTYVLPTWDNTLSYSSNTSCYDLN-PNVNCGYDMMEYCFTFGGPGGPNCEA 344

BLAST of Spg021266 vs. ExPASy TrEMBL
Match: A0A5D3CJM0 (Neprosin 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001660 PE=4 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 1.4e-131
Identity = 244/428 (57.01%), Postives = 309/428 (72.20%), Query Frame = 0

Query: 11  VLFVCFNCKCNQASNSNLSREEELELEAQLKLLNKPSIKTFQTKEGDIIDCVDINKQPAL 70
           + FVCFNCK N ASN NLSREEELE+E QLKLLNKP IKT++TKEGDIIDCVDINKQPAL
Sbjct: 211 LFFVCFNCKFNHASNPNLSREEELEIERQLKLLNKPFIKTYKTKEGDIIDCVDINKQPAL 270

Query: 71  DHPLLKNHKVQTRPSRHLSNLFKDSSESNDNS---FASSIDREPCPVGFVPIRRTLKKDL 130
           DHPLLKNHKVQT PS  +S LFK+ S S  N+    +++ + E CP+GFVPIRRTLK+DL
Sbjct: 271 DHPLLKNHKVQTLPSEFISKLFKEDSISQSNNGILTSNNNNGEGCPIGFVPIRRTLKEDL 330

Query: 131 LRIRSLSSKKPKSRSDQSLNKLEDDYNDYFYDIVKFPYDQNVVSHSMVKGGRYYGADARF 190
           +R++SLSS   K  S  S+   +D   D+ +D V+FPYDQNVVSHS++K   Y+GA AR 
Sbjct: 331 IRLKSLSSNYKKQES--SMKPQDDQSKDFSHDAVRFPYDQNVVSHSLIKATYYHGAKARI 390

Query: 191 AVYDVNVTSD-QSSSSNIWVTGGPSDAALNVLLAGWQANPSINGDSFPRLFVYWTTDAGA 250
           AVY+V+++ + QSSS+NIWV GGP D +LNVL+A      +++GDS PR FVYWTTD GA
Sbjct: 391 AVYNVSLSDENQSSSANIWVVGGP-DESLNVLMA------AVSGDSLPRTFVYWTTDRGA 450

Query: 251 ATGCYNYLCQGFVQVHRSISAGARLSPSSTYQGRQYEFKFGIIQVINTQILNVDVDRNVE 310
            TGCYN LCQGFV V+  I  G+ + P+S YQG+QY+++F I+Q I              
Sbjct: 451 TTGCYNMLCQGFVLVNPDIPVGSSILPASIYQGKQYDYQFSIVQAI-------------- 510

Query: 311 EESNNWWVLVGEKQEAMGYWPSELFPHLRNGSDQVAWGGIAKPS-QDGESPPLGNGHKPN 370
               +WWV VG+ Q  +GYWPSELFP+L  G++QVAWGG A+PS    ESPPLG+GHKPN
Sbjct: 511 ---GHWWVRVGDDQVGLGYWPSELFPNLLRGAEQVAWGGSAEPSLYSDESPPLGSGHKPN 570

Query: 371 KGQYNEGCFIRSIHYLTEGSKVESPNWYNTLSAVSSTDCYGLNATNQNCGYDTFKYCFTY 430
            G+ +E CF+R+I Y+     +  P   NT++ VSS+ CY L  +N+NC +D FKYCFT+
Sbjct: 571 -GRPDEACFVRNIQYIASNYILSIPTLDNTINYVSSSSCYDL-ISNENCDFDPFKYCFTF 610

Query: 431 GGPGGHGC 434
           GGPGG  C
Sbjct: 631 GGPGGQDC 610

BLAST of Spg021266 vs. ExPASy TrEMBL
Match: A0A6J1CVJ6 (uncharacterized protein LOC111014777 OS=Momordica charantia OX=3673 GN=LOC111014777 PE=4 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 1.0e-105
Identity = 213/434 (49.08%), Postives = 266/434 (61.29%), Query Frame = 0

Query: 1   MASKA-TWLVIVLFVCFNCKCNQASNSNLSREEELELEAQLKLLNKPSIKTFQTKEGDII 60
           MASKA  WL+IVL +  NCK + A +SNLSREEELELE QLKLLN+P I TFQT+EGDII
Sbjct: 1   MASKAIMWLMIVLLLHLNCKGSLAFDSNLSREEELELEMQLKLLNRPFITTFQTEEGDII 60

Query: 61  DCVDINKQPALDHPLLKNHKVQTRPSRHLSNLFKDSSESNDNSFASSIDREPCPVGFVPI 120
           DCVDINKQPALDHP LK+HK+QTRPS +   L KDSS S D SF ++ +R  CP G+VPI
Sbjct: 61  DCVDINKQPALDHPSLKSHKIQTRPSTYPFGLSKDSSSSRDKSFINNNNR-ACPAGYVPI 120

Query: 121 RRTLKKDLLRIRSLSSKKPKSRSDQSLNKLEDDYNDYFYDIVKFPYDQNVVSHSMVKGGR 180
           RRT+KKDL+RIRSLSSK+P                      V FPY+Q+VVS +M KG +
Sbjct: 121 RRTIKKDLIRIRSLSSKEPTGIKTSIKGG------------VDFPYNQDVVSVAMKKGIK 180

Query: 181 YYGADARFAVYDVNVTSDQSSSSNIWVTGGPSDAALNVLLAGWQANPSINGDSFPRLFVY 240
           YYGA    +VY+++V  DQSSSSNIW+ GGP  A  NV+LAGWQ NP INGDS  R+FVY
Sbjct: 181 YYGASGSVSVYNLSVAQDQSSSSNIWIIGGPPQAP-NVILAGWQVNPMINGDSLTRMFVY 240

Query: 241 WTTDAGAATGCYNYLCQGFVQVHRSISAGARLSPSSTYQGRQYEFKFGIIQVINTQILNV 300
           WT                                                          
Sbjct: 241 WT---------------------------------------------------------- 300

Query: 301 DVDRNVEEESNNWWVLVGEKQEAMGYWPSELFPHLRNGSDQVAWGGIAKPSQDGESPPLG 360
                 +  + NWW+ VGE  + +GYWP ELF HL +G++QVAWGGIAKPS +G SPPLG
Sbjct: 301 ------DRPTGNWWLAVGESHKTIGYWPKELFGHLNDGTEQVAWGGIAKPSPNGMSPPLG 355

Query: 361 NGHKPNKGQYNEGCFIRSIHYLTEGSKVESPNWYNTLSAVSSTDCYGLNATNQNCGYDTF 420
           NGHKPN  +Y++ C+ R ++Y+ E +K + P   NT + +S+  CY L+   + CG + F
Sbjct: 361 NGHKPNYSKYDDACYFRYMNYVDENNKGQFPANENTANYLSNNSCYALD-NRETCGGEFF 355

Query: 421 KYCFTYGGPGGHGC 434
            YC T+GGPGG+ C
Sbjct: 421 YYCITFGGPGGNNC 355

BLAST of Spg021266 vs. ExPASy TrEMBL
Match: A0A0A0L400 (Neprosin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G011710 PE=4 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 8.5e-84
Identity = 155/292 (53.08%), Postives = 208/292 (71.23%), Query Frame = 0

Query: 146 LNKLEDDYN-DYFYDIVKFPYDQNVVSHSMVKGGRYYGADARFAVYDVNVTSD-QSSSSN 205
           +N  +DD + D FYD V+FPY QNVVSHS++K   Y+GA AR AV++V+++ + QSSS+N
Sbjct: 1   MNPEDDDLSGDSFYDAVRFPYYQNVVSHSLIKAQYYHGAKARIAVHNVSLSDNGQSSSAN 60

Query: 206 IWVTGGPSDAALNVLLAGWQANPSINGDSFPRLFVYWTTDAGAATGCYNYLCQGFVQVHR 265
           IWV GG SD +LNVL+AGWQ NP++NGD+ PR FVYWT D G  TGCYN LCQGFV V+ 
Sbjct: 61  IWVLGG-SDDSLNVLMAGWQVNPAVNGDNLPRTFVYWTVDTGVTTGCYNMLCQGFVLVNP 120

Query: 266 SISAGARLSPSSTYQGRQYEFKFGIIQVINTQILNVDVDRNVEEESNNWWVLVGEKQEAM 325
           +I  G+ + P+S YQG+QY+++F I+Q I                  +WWV VG+ Q  +
Sbjct: 121 NIHVGSSILPASIYQGQQYDYQFSIVQAI-----------------GHWWVRVGDNQVGL 180

Query: 326 GYWPSELFPHLRNGSDQVAWGGIAKPSQDG-ESPPLGNGHKPNKGQYNEGCFIRSIHYLT 385
           GYWP+ELFP+L  G+DQVAWGG A+P+  G ESPPLG+GHKPN G+ +E  F+R+I Y+ 
Sbjct: 181 GYWPNELFPNLLRGADQVAWGGSAQPTLYGDESPPLGSGHKPN-GKPDEAIFVRNIQYIA 240

Query: 386 EGSKVESPNWYNTLSAVSSTDCYGLNATNQNCGYDTFKYCFTYGGPGGHGCQ 435
               +  P   NT++ VS++ CY L  +N+NC +D FKYCFT+GGPGGHGC+
Sbjct: 241 PNYILSIPTLNNTINYVSNSSCYDL-ISNENCSFDPFKYCFTFGGPGGHGCE 272

BLAST of Spg021266 vs. ExPASy TrEMBL
Match: A0A6J1CW60 (uncharacterized protein LOC111014775 OS=Momordica charantia OX=3673 GN=LOC111014775 PE=4 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 1.1e-83
Identity = 175/400 (43.75%), Postives = 220/400 (55.00%), Query Frame = 0

Query: 1   MASKA-TWLVIVLFVCFNCKCNQASNSNLSREEELELEAQLKLLNKPSIKTFQTKEGDII 60
           MASKA  WL+IVL +  NCK + A +SNLS EEELE E QLKLLNKPSI TFQT+EGDII
Sbjct: 1   MASKAIMWLMIVLLLHLNCKGSLAFDSNLSMEEELEFEMQLKLLNKPSITTFQTEEGDII 60

Query: 61  DCVDINKQPALDHPLLKNHKVQTRPSRHLSNLFKDSSESNDNSFASSIDREPCPVGFVPI 120
           DCVDINKQPALDHPLLKNHKVQ                                      
Sbjct: 61  DCVDINKQPALDHPLLKNHKVQ-------------------------------------- 120

Query: 121 RRTLKKDLLRIRSLSSKKPKSRSDQSLNKLEDDYNDYFYDIVKFPYDQNVVSHSMVKGGR 180
                                                            V S +M +G +
Sbjct: 121 -------------------------------------------------VASVAMKRGKK 180

Query: 181 YYGADARFAVYDVNVTSDQSSSSNIWVTGGPSDAALNVLLAGWQANPSINGDSFPRLFVY 240
           YYG     +VY+++V  DQSSSSNIW+ GGP +A  NV+L GWQ NP INGDS  R+FVY
Sbjct: 181 YYGDARSVSVYNLSVAQDQSSSSNIWIIGGPPEAP-NVILTGWQVNPMINGDSRTRMFVY 240

Query: 241 WTTDAGAATGCYNYLCQGFVQVHRSISAGARLSPSSTYQGRQYEFKFGIIQVINTQILNV 300
           WT D G  TG YN  C+ F+Q + S      L PSSTYQG+QY++ F + Q         
Sbjct: 241 WTADGGIHTGYYNMFCRCFIQTNPSTPPNIPLYPSSTYQGKQYDYTFTVFQ--------- 297

Query: 301 DVDRNVEEESNNWWVLVGEKQEAMGYWPSELFPHLRNGSDQVAWGGIAKPSQDGESPPLG 360
                 +  + +WW+ V + Q  +GYWP ELF HL +G++QVAWGGIAKPS +G SPPLG
Sbjct: 301 ------DRPTGDWWLAVSDSQTTIGYWPKELFGHLNDGAEQVAWGGIAKPSPNGMSPPLG 297

Query: 361 NGHKPNKGQYNEGCFIRSIHYLTEGSKVESPNWYNTLSAV 400
           NGHKPN G++++ C+ R+++Y+ E ++ E     NT S +
Sbjct: 361 NGHKPNYGKHDDACYFRTLNYINENNESEVSAIENTASYI 297

BLAST of Spg021266 vs. ExPASy TrEMBL
Match: A0A5A7UEV4 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G001630 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 1.7e-79
Identity = 153/289 (52.94%), Postives = 197/289 (68.17%), Query Frame = 0

Query: 150 EDDYNDYFYDIVKFPYDQNVVSHSMVKG-GRYYGADARFAVYDVNVTSDQSSSSNIWVTG 209
           +D  +D+F D VK+P +QNVVSHS+ KG  +YYG  +  +VY+V+++  QSSSSNIW+ G
Sbjct: 5   DDQSDDFFDDSVKYPDNQNVVSHSLKKGPEKYYGTKSYMSVYNVSLSFGQSSSSNIWIVG 64

Query: 210 GPSDAALNVLLAGWQANPSINGDSFPRLFVYWTTDAGAATGCYNYLCQGFVQVHRSISAG 269
           GP++ +L VL+ GW  NP +NGD   R FVYWT D GA TGCYN  CQGFVQV+ S   G
Sbjct: 65  GPTN-SLGVLMTGWLVNPEVNGDFITRSFVYWTADGGATTGCYNMYCQGFVQVNPSHHVG 124

Query: 270 ARLSPSSTYQGRQYEFKFGIIQVINTQILNVDVDRNVEEESNNWWVLVGEKQEAMGYWPS 329
           A L P+STYQG+QY+++F IIQ+                 + NWWVLVGE    +GYWP 
Sbjct: 125 APLHPTSTYQGQQYDYQFTIIQI-----------------AGNWWVLVGENL-GLGYWPK 184

Query: 330 ELFPHLRNGSDQVAWGGIAKPSQDGESPPLGNGHKPN-KGQYNEGCFIRSIHYLTEG--S 389
           EL  +L +G++Q+AWGGIAKPS DG SP LG+GHKPN  G YNEGC+IR+I  ++    +
Sbjct: 185 ELVQNLVDGAEQIAWGGIAKPSIDGMSPMLGSGHKPNDNGDYNEGCYIRNIQIISGAATN 244

Query: 390 KVESPNWYNTLSAVSSTDCYGLNATNQNCGYDTFKYCFTYGGPGGHGCQ 435
             + P W NTLS  S+T CY LN  N NCG D  +YCFT+GGPGG  C+
Sbjct: 245 TYKLPTWDNTLSYSSNTSCYDLN-PNVNCGDDMMEYCFTFGGPGGPNCE 273

BLAST of Spg021266 vs. TAIR 10
Match: AT3G13510.1 (Protein of Unknown Function (DUF239) )

HSP 1 Score: 237.7 bits (605), Expect = 1.8e-62
Identity = 142/430 (33.02%), Postives = 224/430 (52.09%), Query Frame = 0

Query: 10  IVLFVCFNCKCNQASNSNLSREEELELEAQLKLLNKPSIKTFQTKEGDIIDCVDINKQPA 69
           + L+V  +  C  AS    S  ++ E++  L  LNKP +KT Q+ +GDIIDC+ I+KQPA
Sbjct: 17  VCLWVMLSLSCAAASYG--SSRQKFEVKKHLNRLNKPPVKTIQSPDGDIIDCIPISKQPA 76

Query: 70  LDHPLLKNHKVQTRPSRHLSNLFKDSSESNDNSFASSIDRE------PCPVGFVPIRRTL 129
            DHP LK+HK+Q RPS H   LF D+  S +     +   +       C  G +P+RRT 
Sbjct: 77  FDHPFLKDHKIQMRPSYHPEGLFDDNKVSAEPKGKETHIPQLWHRYGKCTEGTIPMRRTR 136

Query: 130 KKDLLRIRSLSSKKPKSRSDQSLNKLEDDYNDYFYDIVKFPYDQNVVSHSMVKGGRYYGA 189
           + D+LR  S+     K      + K  +       D++     Q+ +++  V+G +YYGA
Sbjct: 137 EDDVLRASSVKRYGKKKHRSVPIPKSAEP------DLINQNGHQHAIAY--VEGDKYYGA 196

Query: 190 DARFAVYDVNV-TSDQSSSSNIWVTGGPSDAALNVLLAGWQANPSINGDSFPRLFVYWTT 249
            A   V++  +  +++ S S IW+ GG     LN + AGWQ +P + GD+  RLF YWT+
Sbjct: 197 KATLNVWEPKIQNTNEFSLSQIWLLGGSFGQDLNSIEAGWQVSPDLYGDNNTRLFTYWTS 256

Query: 250 DAGAATGCYNYLCQGFVQVHRSISAGARLSPSSTYQGRQYEFKFGIIQVINTQILNVDVD 309
           DA  ATGCYN LC GF+Q++  I+ GA +SP S Y+  QY+    I +            
Sbjct: 257 DAYQATGCYNLLCSGFIQINSDIAMGASISPVSGYRNSQYDISILIWK------------ 316

Query: 310 RNVEEESNNWWVLVGEKQEAMGYWPSELFPHLRNGSDQVAWGG-IAKPSQDGES--PPLG 369
              + +  +WW+  G     +GYWPS LF +L   +  + WGG +     +G      +G
Sbjct: 317 ---DPKEGHWWMQFGNGY-VLGYWPSFLFSYLTESASMIEWGGEVVNSQSEGHHTWTQMG 376

Query: 370 NGHKPNKGQYNEGCFIRSIHYLTEGSKVESPNWYNTLSAVSSTDCYGLNATNQNCGYDTF 429
           +GH P +G +++  + R+I  +   + +++P    T +    ++CY +    Q    D +
Sbjct: 377 SGHFPEEG-FSKASYFRNIQVVDGSNNLKAPKGLGTFT--EKSNCYDV----QTGSNDDW 413

BLAST of Spg021266 vs. TAIR 10
Match: AT1G55360.1 (Protein of Unknown Function (DUF239) )

HSP 1 Score: 236.5 bits (602), Expect = 4.1e-62
Identity = 141/418 (33.73%), Postives = 223/418 (53.35%), Query Frame = 0

Query: 23  ASNSNLSREEELELEAQLKLLNKPSIKTFQTKEGDIIDCVDINKQPALDHPLLKNHKVQT 82
           A+ S +S+ ++ E++  L  LNKP++K+ Q+ +GD+IDCV I+KQPA DHP LK+HK+Q 
Sbjct: 31  AARSGVSK-QKFEVKKHLNRLNKPAVKSIQSSDGDVIDCVPISKQPAFDHPFLKDHKIQM 90

Query: 83  RPSRHLSNLFKDS--SESNDNSFASSIDR-----EPCPVGFVPIRRTLKKDLLRIRSLSS 142
           +P+ H   LF D+  S    N     I +       C  G +P+RRT + D+LR  S+  
Sbjct: 91  KPNYHPEGLFDDNKVSAPKSNEKEGHIPQLWHRYGKCSEGTIPMRRTKEDDVLRASSVKR 150

Query: 143 KKPKSRSDQSLNKLEDDYNDYFYDIVKFPYDQNVVSHSMVKGGRYYGADARFAVYDVNV- 202
              K R    L K  +       D++     Q+ +++  V+G +YYGA A   V++  + 
Sbjct: 151 YGKKKRRSVPLPKSAEP------DLINQSGHQHAIAY--VEGDKYYGAKATINVWEPKIQ 210

Query: 203 TSDQSSSSNIWVTGGPSDAALNVLLAGWQANPSINGDSFPRLFVYWTTDAGAATGCYNYL 262
             ++ S S IW+ GG     LN + AGWQ +P + GD+  RLF YWT+DA  ATGCYN L
Sbjct: 211 QQNEFSLSQIWLLGGSFGQDLNSIEAGWQVSPDLYGDNNTRLFTYWTSDAYQATGCYNLL 270

Query: 263 CQGFVQVHRSISAGARLSPSSTYQGRQYEFKFGIIQVINTQILNVDVDRNVEEESNNWWV 322
           C GF+Q++  I+ GA +SP S Y+  QY+    I +               + +  +WW+
Sbjct: 271 CSGFIQINSDIAMGASISPVSGYRNSQYDISILIWK---------------DPKEGHWWM 330

Query: 323 LVGEKQEAMGYWPSELFPHLRNGSDQVAWGG-IAKPSQDGE--SPPLGNGHKPNKGQYNE 382
             G     +GYWPS LF +L   +  + WGG +     DG+  S  +G+G  P +G +++
Sbjct: 331 QFGNGY-VLGYWPSFLFSYLTESASMIEWGGEVVNSQSDGQHTSTQMGSGKFPEEG-FSK 390

Query: 383 GCFIRSIHYLTEGSKVESPNWYNTLSAVSSTDCYGLNATNQNCGYDTFKYCFTYGGPG 430
             + R+I  +   + +++P    T +    ++CY +    Q    D + + F YGGPG
Sbjct: 391 ASYFRNIQVVDGSNNLKAPKGLGTFT--EQSNCYDV----QTGSNDDWGHYFYYGGPG 416

BLAST of Spg021266 vs. TAIR 10
Match: AT5G56530.1 (Protein of Unknown Function (DUF239) )

HSP 1 Score: 233.8 bits (595), Expect = 2.6e-61
Identity = 142/423 (33.57%), Postives = 224/423 (52.96%), Query Frame = 0

Query: 23  ASNSNLSREEELELEAQLKLLNKPSIKTFQTKEGDIIDCVDINKQPALDHPLLKNHKVQT 82
           A   ++SR +  E+   L  LNKP++K+ Q+ +GDIIDCV I+KQPA DHP LK+HK+Q 
Sbjct: 30  AGRLSVSR-QNFEVHKHLNRLNKPAVKSIQSPDGDIIDCVHISKQPAFDHPFLKDHKIQM 89

Query: 83  RPSRHLSNLFKDSSESNDNSFASS------IDREPCPVGFVPIRRTLKKDLLRIRSLSSK 142
            PS    +LF +S  S     + +           C  G +P+RRT K+D+LR  S+   
Sbjct: 90  GPSYTPESLFGESKVSEKPKESVNPITQLWHQNGVCSEGTIPVRRTKKEDVLRASSVKRY 149

Query: 143 KPKSRSDQSLNKLEDDYNDYFYDIVKFPYDQNVVSHSMVKGGRYYGADARFAVYDVNV-T 202
             K      L +  D       D++     Q+ +++  V+GG++YGA A   V++  V +
Sbjct: 150 GKKKHLSVPLPRSADP------DLINQSGHQHAIAY--VEGGKFYGAKATINVWEPKVQS 209

Query: 203 SDQSSSSNIWVTGGPSDAALNVLLAGWQANPSINGDSFPRLFVYWTTDAGAATGCYNYLC 262
           S++ S S +W+ GG     LN + AGWQ +P + GD+  RLF YWT+DA  ATGCYN LC
Sbjct: 210 SNEFSLSQLWILGGSFGQDLNSIEAGWQVSPDLYGDNNTRLFTYWTSDAYQATGCYNLLC 269

Query: 263 QGFVQVHRSISAGARLSPSSTYQGRQYEFKFGIIQVINTQILNVDVDRNVEEESNNWWVL 322
            GF+Q++  I+ GA +SP S +   QY+    I +               + +  +WW+ 
Sbjct: 270 SGFIQINSQIAMGASISPVSGFHNPQYDISITIWK---------------DPKEGHWWMQ 329

Query: 323 VGEKQEAMGYWPSELFPHLRNGSDQVAWGG-IAKPSQDGE--SPPLGNGHKPNKGQYNEG 382
            G+    +GYWPS LF +L + +  V WGG +    +DG   +  +G+G  P++G + + 
Sbjct: 330 FGDGY-VLGYWPSFLFSYLADSASIVEWGGEVVNMEEDGHHTTTQMGSGQFPDEG-FTKA 389

Query: 383 CFIRSIHYLTEGSKVESPNWYNTLSAVSSTDCYGLNATNQNCGYDTFKYCFTYGGPGGH- 435
            + R+I  +   + ++ P   NT +    ++CY +         D + + F YGGPG + 
Sbjct: 390 SYFRNIQVVDSSNNLKEPKGLNTFT--EKSNCYDVEVGKN----DDWGHYFYYGGPGRNP 420

BLAST of Spg021266 vs. TAIR 10
Match: AT5G56530.2 (Protein of Unknown Function (DUF239) )

HSP 1 Score: 233.8 bits (595), Expect = 2.6e-61
Identity = 142/423 (33.57%), Postives = 224/423 (52.96%), Query Frame = 0

Query: 23  ASNSNLSREEELELEAQLKLLNKPSIKTFQTKEGDIIDCVDINKQPALDHPLLKNHKVQT 82
           A   ++SR +  E+   L  LNKP++K+ Q+ +GDIIDCV I+KQPA DHP LK+HK+Q 
Sbjct: 30  AGRLSVSR-QNFEVHKHLNRLNKPAVKSIQSPDGDIIDCVHISKQPAFDHPFLKDHKIQM 89

Query: 83  RPSRHLSNLFKDSSESNDNSFASS------IDREPCPVGFVPIRRTLKKDLLRIRSLSSK 142
            PS    +LF +S  S     + +           C  G +P+RRT K+D+LR  S+   
Sbjct: 90  GPSYTPESLFGESKVSEKPKESVNPITQLWHQNGVCSEGTIPVRRTKKEDVLRASSVKRY 149

Query: 143 KPKSRSDQSLNKLEDDYNDYFYDIVKFPYDQNVVSHSMVKGGRYYGADARFAVYDVNV-T 202
             K      L +  D       D++     Q+ +++  V+GG++YGA A   V++  V +
Sbjct: 150 GKKKHLSVPLPRSADP------DLINQSGHQHAIAY--VEGGKFYGAKATINVWEPKVQS 209

Query: 203 SDQSSSSNIWVTGGPSDAALNVLLAGWQANPSINGDSFPRLFVYWTTDAGAATGCYNYLC 262
           S++ S S +W+ GG     LN + AGWQ +P + GD+  RLF YWT+DA  ATGCYN LC
Sbjct: 210 SNEFSLSQLWILGGSFGQDLNSIEAGWQVSPDLYGDNNTRLFTYWTSDAYQATGCYNLLC 269

Query: 263 QGFVQVHRSISAGARLSPSSTYQGRQYEFKFGIIQVINTQILNVDVDRNVEEESNNWWVL 322
            GF+Q++  I+ GA +SP S +   QY+    I +               + +  +WW+ 
Sbjct: 270 SGFIQINSQIAMGASISPVSGFHNPQYDISITIWK---------------DPKEGHWWMQ 329

Query: 323 VGEKQEAMGYWPSELFPHLRNGSDQVAWGG-IAKPSQDGE--SPPLGNGHKPNKGQYNEG 382
            G+    +GYWPS LF +L + +  V WGG +    +DG   +  +G+G  P++G + + 
Sbjct: 330 FGDGY-VLGYWPSFLFSYLADSASIVEWGGEVVNMEEDGHHTTTQMGSGQFPDEG-FTKA 389

Query: 383 CFIRSIHYLTEGSKVESPNWYNTLSAVSSTDCYGLNATNQNCGYDTFKYCFTYGGPGGH- 435
            + R+I  +   + ++ P   NT +    ++CY +         D + + F YGGPG + 
Sbjct: 390 SYFRNIQVVDSSNNLKEPKGLNTFT--EKSNCYDVEVGKN----DDWGHYFYYGGPGRNP 420

BLAST of Spg021266 vs. TAIR 10
Match: AT1G70550.2 (Protein of Unknown Function (DUF239) )

HSP 1 Score: 213.4 bits (542), Expect = 3.7e-55
Identity = 140/423 (33.10%), Postives = 215/423 (50.83%), Query Frame = 0

Query: 23  ASNSNLSREEELE----LEAQLKLLNKPSIKTFQTKEGDIIDCVDINKQPALDHPLLKNH 82
           A++  L  +EEL+    +  +L  +NKP++KT Q+ +GD IDCV  ++QPA DHPLL+  
Sbjct: 31  AADQTLRPQEELQKLTLIRQELDKINKPAVKTIQSSDGDKIDCVSTHQQPAFDHPLLQGQ 90

Query: 83  KVQTRPSRHLSNLFKDSSESNDNSFASSIDREPCPVGFVPIRRTLKKDLLRIRSLSSKKP 142
           K    P   +   + +   S +NS   S+  E CP G +PIRRT ++D+LR  S+     
Sbjct: 91  KPLDPP--EIPKGYSEDDGSYENSQLWSLSGESCPEGTIPIRRTTEQDMLRASSV----- 150

Query: 143 KSRSDQSLNKLEDDYNDYFYDIVKFPYDQNVVSHSMVKGGRYYGADARFAVYDVNVTSD- 202
             R  + + +++ D  +  +        ++ V +  V G +YYGA A   V+   VTS  
Sbjct: 151 -QRFGRKIRRVKRDSTNNGH--------EHAVGY--VTGRQYYGAKASINVWSPRVTSQY 210

Query: 203 QSSSSNIWVTGGPSDAALNVLLAGWQANPSINGDSFPRLFVYWTTDAGAATGCYNYLCQG 262
           + S S IWV  G     LN + AGWQ +P + GD++PR F YWT+DA   TGCYN LC G
Sbjct: 211 EFSLSQIWVIAGSFTHDLNTIEAGWQISPELYGDTYPRFFTYWTSDAYRTTGCYNLLCSG 270

Query: 263 FVQVHRSISAGARLSPSSTYQGRQYEFKFGIIQVINTQILNVDVDRNVEEESNNWWVLVG 322
           FVQ +R I+ GA +SP S+Y+G Q++    I +               + +  +WW+  G
Sbjct: 271 FVQTNRRIAIGAAISPRSSYKGGQFDISLLIWK---------------DPKHGHWWLQFG 330

Query: 323 EKQEAMGYWPSELFPHLRNGSDQVAWGGIA---KPSQDGESPPLGNGHKPNKGQYNEGCF 382
                +GYWP+ LF HL+     V +GG     +P     +  +G+GH   +G + +  +
Sbjct: 331 -SGALVGYWPAFLFTHLKQHGSMVQFGGEIVNNRPGGSHTTTQMGSGHFAGEG-FGKASY 390

Query: 383 IRSIHYLTEGSKVESPNWYNTLSAVSS-------TDCYGL-NATNQNCGYDTFKYCFTYG 430
            R++  +         +W NTL   S+        +CY +   TN+  G       F YG
Sbjct: 391 FRNLQIV---------DWDNTLIPASNLKILADHPNCYDIRGGTNRVWG-----NYFYYG 404