Spg021078 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg021078 vs. NCBI nr
Match: XP_004146562.1 (subtilisin-like protease SBT3.6 [Cucumis sativus] >KGN53334.1 hypothetical protein Csa_014762 [Cucumis sativus])

HSP 1 Score: 995.0 bits (2571), Expect = 3.7e-286
Identity = 523/780 (67.05%), Postives = 611/780 (78.33%), Query Frame = 0

Query: 8   LIVGVITIYALFSMFAYKP-MAEADDQNRKVYIVYLGERQYEDSKLTTESHHDLLATVLG 67
           LIVG I    LF     KP +AEADDQN KV+IVYLGE+ + D+K T +SHH LL+T+LG
Sbjct: 10  LIVGFIIFDCLF-----KPILAEADDQNPKVHIVYLGEKPHHDTKFTIDSHHQLLSTILG 69

Query: 68  SKEKSSESMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQTTRSWDFLGL 127
           SKEKS E+MVYSY+HGFSG AAKLTKSQAQKL+EM  VVRV+P+S YK+ TTRSWDFLGL
Sbjct: 70  SKEKSMEAMVYSYKHGFSGFAAKLTKSQAQKLSEMSRVVRVVPSSLYKVHTTRSWDFLGL 129

Query: 128 SSSP-SNSNLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGRCESGAEFNS 187
           SSSP  +SNLL R +MG+ +IIGVID+GIWPESE+F+D G+G IPSRWKG CESG +FNS
Sbjct: 130 SSSPFESSNLLHRAQMGENVIIGVIDTGIWPESESFKDKGVGSIPSRWKGTCESGEQFNS 189

Query: 188 TNCNRKIIGARWYSKGFIDNYGAEALANEYLSPRDANGHGTHVASTAAGSFVTNIGYHGL 247
           TNCN+KIIGARW+ KGF+ + G +ALA EYLSPRD NGHGTH AS AAGSFV NI YH  
Sbjct: 190 TNCNKKIIGARWFMKGFVADLGRDALAKEYLSPRDLNGHGTHTASIAAGSFVANINYHNN 249

Query: 248 GIGTVRGGAPLARLAIYKVLWTNSGQGSIADILLAIDEAIHDGVDVLSMSIVGSIPSLPE 307
             GTVRGGAPLARLAIYK LWT    GS ADIL AIDEAI+DGVDVLSMSI    P LPE
Sbjct: 250 AAGTVRGGAPLARLAIYKALWTKDAVGSTADILKAIDEAINDGVDVLSMSIGSLTPFLPE 309

Query: 308 FDELHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTMDRAFLASI- 367
           F+E +  +A G FHAIA+GISVVCA GN GP  QT+EN APWI TVAA+T+DRAFLASI 
Sbjct: 310 FNEAN-DIAFGSFHAIAKGISVVCAAGNSGPTPQTVENVAPWIFTVAANTIDRAFLASIT 369

Query: 368 ALGDNTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDGIFGNETFLSG 427
            L DNTT+LGQSL  +KKD+V +L    T                GRCD + GNETF++G
Sbjct: 370 TLPDNTTFLGQSLLDSKKDLVAELETLDT----------------GRCDDLLGNETFING 429

Query: 428 KVVLCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDNL---LPFAIPCILVDSD 487
           KVV+CFS+     TI++    A MAVA+A G GIIVAGQQ+D+L   +P  IPCILVD+D
Sbjct: 430 KVVMCFSNLADHNTIYD----AAMAVARANGTGIIVAGQQDDDLFSCIPSPIPCILVDTD 489

Query: 488 VGTKLYFYAL--HNSNPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKPDIAAPG 547
           VG+KL+F  L  +++NPVVRLR  RTIIGKPI+  I+YFSSRGPNSVS  ILKPDI+APG
Sbjct: 490 VGSKLFFINLLQNSTNPVVRLRATRTIIGKPITPAISYFSSRGPNSVSNPILKPDISAPG 549

Query: 548 SNIVAAISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSALITTASA 607
           SNI+AA+SPH+ F+ KGF + SGTSMA PHIS IVALLKS+HPTWSPAAIKSAL+TTA  
Sbjct: 550 SNILAAVSPHHIFNEKGFMLLSGTSMATPHISAIVALLKSVHPTWSPAAIKSALMTTART 609

Query: 608 KDPSGLPIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYI-YYLCAMGYQDS 667
           +   GLPIFAEG+PPK+ADPFDYGGG+V+ NAA DPGL+YD+   DYI YYLC MGY+D 
Sbjct: 610 EVSPGLPIFAEGTPPKMADPFDYGGGIVDANAAVDPGLVYDMGRKDYIDYYLCGMGYKDE 669

Query: 668 EISSITGQKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRAVIDPPP 727
           +IS +T +K  CPLQ+ SVLDLNLP ITIP+L NST VTRTVTNVGNL+ VY+A I+ P 
Sbjct: 670 DISHLTQRKTVCPLQRLSVLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAEIESPF 729

Query: 728 GTKVSVEPSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPLSVRINF 779
           G KVSV P  L F+SQVKKISFKV   T V RNYGYSFG LTW+DG+H+V+ PLSVR  F
Sbjct: 730 GCKVSVNPQVLVFNSQVKKISFKVMFFTQVQRNYGYSFGRLTWTDGIHVVKIPLSVRFGF 763

BLAST of Spg021078 vs. NCBI nr
Match: XP_022984814.1 (subtilisin-like protease SBT3.9 [Cucurbita maxima])

HSP 1 Score: 995.0 bits (2571), Expect = 3.7e-286
Identity = 522/783 (66.67%), Postives = 615/783 (78.54%), Query Frame = 0

Query: 1   MVNGQAP-LIVGVITIYALFSMFAYKPMAEADDQNRKVYIVYLGERQYEDSKLTTESHHD 60
           M N +AP  I+ V+TIYA+FS      MAEADDQN KV+IVYLGER Y+D KLTT+SHH+
Sbjct: 1   MENNRAPSFILAVVTIYAVFS-----DMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHE 60

Query: 61  LLATVLGSKEKSSESMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQTTR 120
           LL +VLGSKEKS ESMVYSYRHGFSG AAKLT SQAQKLA MP V RV P+S YKM+TTR
Sbjct: 61  LLESVLGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTR 120

Query: 121 SWDFLGLSSSPS-NSNLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGRCE 180
           SWDFLGLSSSPS +SNL  R KMGD +IIGVID+G WPESE+F D G+GPIPSRWKG C+
Sbjct: 121 SWDFLGLSSSPSESSNLFHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQ 180

Query: 181 SGAEFNSTNCNRKIIGARWYSKGFIDNYGAEALANEYLSPRDANGHGTHVASTAAGSFVT 240
           SG +FNS++CN+K+IGARW++   I ++G EA+  +YLS RD  GHGTH ASTA G+FV 
Sbjct: 181 SGEDFNSSHCNKKVIGARWFASALIADHGEEAVFKDYLSARDNEGHGTHTASTAGGAFVR 240

Query: 241 NIGYHGLGIGTVRGGAPLARLAIYKVLWTNSGQGSIADILLAIDEAIHDGVDVLSMSIVG 300
           N+ Y G G GT+RGGAPLARLAIYKVLW++S  GS ADIL  IDEAIHDGVDVLSMSI  
Sbjct: 241 NVSYFGNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIGK 300

Query: 301 SIPSLPEFDELHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTMDR 360
           SIP  P+ ++++  VA+G FHAIA+GISVVCAGGN+G +QQT+EN APW+ TVAAST+DR
Sbjct: 301 SIPLFPDVNDVN-PVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDR 360

Query: 361 AFLASI-ALGDNTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDGIFG 420
           AFL SI  LGDN TYLGQ     +KD+VG LV                  + GRC GI G
Sbjct: 361 AFLVSITTLGDNATYLGQMFL--RKDIVGMLVA-----------------MDGRCAGILG 420

Query: 421 NETFLSGKVV-LCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDNLLPFAIPCI 480
            +  +SG VV LCF+D   T       + AVM   +AK  G+I AGQQ DNL+P  IPCI
Sbjct: 421 GDIPISGNVVLLCFTDLAKT----APASNAVMPGKQAKVVGVIYAGQQTDNLVPCDIPCI 480

Query: 481 LVDSDVGTKLYFYALHNSNPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKPDIA 540
            VD+ VGTKL+ Y L++ + ++RLR  RTIIGKPISS+IAYFSSRGPNS SP ILKPDIA
Sbjct: 481 HVDTHVGTKLFTYLLNDDDALIRLRATRTIIGKPISSRIAYFSSRGPNSFSPGILKPDIA 540

Query: 541 APGSNIVAAISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSALITT 600
           APG+NI+AA+ P++    KGFA  SGTSMA PHISGIV L+KSLHPTWSPAAIKSALITT
Sbjct: 541 APGANIIAAVPPNHKGGDKGFATMSGTSMATPHISGIVTLIKSLHPTWSPAAIKSALITT 600

Query: 601 ASAKDPSGLPIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYI-YYLCAMGY 660
           A  +DPSG+PIFAEGSPPKVADPFDYGGGVV+ NAA DPGLIYDL  TDYI YYLC+MGY
Sbjct: 601 ARVEDPSGMPIFAEGSPPKVADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGY 660

Query: 661 QDSEISSITGQKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRAVID 720
            +++IS ++ QK  CP ++ S+LDLNLPTIT+PAL NSTTVTRTVTNVGNLT+VY+AVI 
Sbjct: 661 TEADISHLSQQKTVCPSKRASILDLNLPTITVPALINSTTVTRTVTNVGNLTAVYKAVIK 720

Query: 721 PPPGTKVSVEPSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPLSVR 779
            PPG+KV V+P  L F+S VKKISFKV  S+ + RNYGYSFG+LTW+DGVHLV+SPLSVR
Sbjct: 721 APPGSKVRVKPRVLVFNSNVKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVR 754

BLAST of Spg021078 vs. NCBI nr
Match: XP_023552781.1 (subtilisin-like protease SBT3.7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 992.6 bits (2565), Expect = 1.9e-285
Identity = 529/785 (67.39%), Postives = 616/785 (78.47%), Query Frame = 0

Query: 1   MVNGQAP---LIVGVITIYALFSMFAYKPMAEADDQNRKVYIVYLGERQYEDSKLTTESH 60
           M N QAP   L+V V+TIY +FS      MAEADDQN KV+IVYLGER Y+D KLTT+SH
Sbjct: 1   MENNQAPSFMLVVAVVTIYGVFS-----AMAEADDQNSKVHIVYLGERPYDDVKLTTDSH 60

Query: 61  HDLLATVLGSKEKSSESMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQT 120
           H+LL +VLGSKEKS ES+VYSYRHGFSG AAKLT SQAQKLA MP V RV P+  YKM+T
Sbjct: 61  HELLESVLGSKEKSLESIVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSPLYKMRT 120

Query: 121 TRSWDFLGLSSSPS-NSNLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGR 180
           TRSWDFLGLSSSPS +SNLL R KMGD +IIGVID+G WPESE+F D G+GPIPSRWKG 
Sbjct: 121 TRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGI 180

Query: 181 CESGAEFNSTNCNRKIIGARWYSKGFIDNYGAEALANEYLSPRDANGHGTHVASTAAGSF 240
           CESG +FNS++CN+K+IGARW++   + ++G EA+  +YLS RD  GHGTH ASTA G+F
Sbjct: 181 CESGEDFNSSHCNKKVIGARWFASALVADHGEEAVFMDYLSARDNQGHGTHTASTAGGAF 240

Query: 241 VTNIGYHGLGIGTVRGGAPLARLAIYKVLWTNSGQGSIADILLAIDEAIHDGVDVLSMSI 300
           V N+   G G GT+RGGAPLARLAIYKVLW++S  GS ADIL  IDEAIHDGVDVLSMSI
Sbjct: 241 VRNVSLFGNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSI 300

Query: 301 VGSIPSLPEFDELHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTM 360
             SIP   + +EL+  VA+G FHAIA+GISVVCAGGN+G +QQT+EN APW+ TVAAST+
Sbjct: 301 GKSIPLFSDVNELN-PVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTI 360

Query: 361 DRAFLASI-ALGDNTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDGI 420
           DRAFLASI  LGDN TYLGQ+    KKD+VG L+                  + GRC G+
Sbjct: 361 DRAFLASITTLGDNATYLGQTFL--KKDIVGMLLA-----------------MDGRCAGL 420

Query: 421 FGNETFLSGKVV-LCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDNLLPFAIP 480
            G+   +SG VV LCF+D +A K     V   VM   +AK  GII AGQ ND L P   P
Sbjct: 421 LGSNIHISGNVVLLCFTD-LAKKAGASNV---VMPGKQAKVVGIIYAGQHNDILGPCDTP 480

Query: 481 CILVDSDVGTKLYFYALHNSNPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKPD 540
           CI VD+ VGTKL+ Y L++    +RLR  RTI GKPISS+IAYFSSRGPNS+SP ILKPD
Sbjct: 481 CIHVDTHVGTKLFTYYLNDEKAFIRLRATRTITGKPISSRIAYFSSRGPNSISPGILKPD 540

Query: 541 IAAPGSNIVAAISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSALI 600
           IAAPGSNI+AA+ P++  S KGFA  SGTSMAAPHISGIVAL+KSL PTWSPAAIKSALI
Sbjct: 541 IAAPGSNIIAAVPPNHKGSDKGFAAMSGTSMAAPHISGIVALIKSLRPTWSPAAIKSALI 600

Query: 601 TTASAKDPSGLPIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYI-YYLCAM 660
           TTA  +DPSGLPIFAEGSPPKVADPFDYGGGVV+ NAA DPGLIYDL  TDYI YYLC+M
Sbjct: 601 TTARVEDPSGLPIFAEGSPPKVADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSM 660

Query: 661 GYQDSEISSITGQKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRAV 720
           GY ++EIS ++ QK  CP ++ SVLDLNLPTIT+PALTNSTTVTRTVTNVGNLT+VY+AV
Sbjct: 661 GYTEAEISHLSQQKTVCPSKRASVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAV 720

Query: 721 IDPPPGTKVSVEPSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPLS 779
           I  PPG+KV V+P  LAF+S VKKISFKV  S+++ RNYGYSFG+LTW+DGVHLV+SPLS
Sbjct: 721 IKAPPGSKVRVKPRVLAFNSNVKKISFKVKFSSSLQRNYGYSFGSLTWTDGVHLVKSPLS 756

BLAST of Spg021078 vs. NCBI nr
Match: XP_008452040.1 (PREDICTED: subtilisin-like protease SBT3.7 isoform X1 [Cucumis melo])

HSP 1 Score: 978.0 bits (2527), Expect = 4.7e-281
Identity = 521/786 (66.28%), Postives = 604/786 (76.84%), Query Frame = 0

Query: 2   VNGQ-APLIVGVITIYALFSMFAYKPMAEADDQNRKVYIVYLGERQYEDSKLTTESHHDL 61
           +N Q A LIVG I  Y L        +AE++DQN KV+IVYLGE+ + D+K TT+SHH L
Sbjct: 3   INSQLAHLIVGFIIFYGLCEPI----LAESNDQNPKVHIVYLGEKPHHDTKFTTDSHHQL 62

Query: 62  LATVLGSKEKSSESMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQTTRS 121
           LA +LGSKEKS E+MVYSY+HGFSG AAKLTKS+AQKL+EM  VVRV+P+S YK+ TTRS
Sbjct: 63  LAAILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEAQKLSEMSRVVRVVPSSLYKVHTTRS 122

Query: 122 WDFLGLSSSPS-NSNLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGRCES 181
           WDFLGLSSSPS +SNLL R K GD +IIGVIDSGIWPESE+F+D GLGPIPSRWKG CES
Sbjct: 123 WDFLGLSSSPSESSNLLHRAK-GDDVIIGVIDSGIWPESESFKDKGLGPIPSRWKGTCES 182

Query: 182 GAEFNSTNCNRKIIGARWYSKGFIDNYGAEALANEYLSPRDANGHGTHVASTAAGSFVTN 241
           G +FNSTNCN+KIIGARW+ K F+ +YG EALA EYLSPRD +GHGTH ASTAAGSFV N
Sbjct: 183 GEQFNSTNCNKKIIGARWFVKAFVADYGREALAKEYLSPRDLHGHGTHTASTAAGSFVAN 242

Query: 242 IGYHGLGIGTVRGGAPLARLAIYKVLWTNSGQGSIADILLAIDEAIHDGVDVLSMSIVGS 301
           I YH    GT RGGAPLARLAIYK LWTN G GS ADIL AIDEAIHDGVDVLS+SI GS
Sbjct: 243 INYHNNAAGTARGGAPLARLAIYKALWTNRGVGSSADILKAIDEAIHDGVDVLSISIGGS 302

Query: 302 IPSLPEFDELHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTMDRA 361
            P  PEF EL   +A G FHAI +GISVVCA GN GP  Q ++N APWI TVA +T+DRA
Sbjct: 303 PPFYPEFTEL-SDIAFGSFHAITKGISVVCAAGNSGPSPQMVDNVAPWIFTVATNTIDRA 362

Query: 362 FLASI-ALGDNTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDGIFGN 421
           FL+SI  L DNTT++GQSL  +KKD+V +LV              W      RCD + GN
Sbjct: 363 FLSSITTLRDNTTFMGQSLLESKKDLVAELV-------------SW-----ERCDQLSGN 422

Query: 422 ETFLSGKVVLCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDN----LLPFAIP 481
           E F++GKVVLCF         + T++ A   V +A G GIIVAGQQ DN     +   IP
Sbjct: 423 EAFINGKVVLCF----PKLADYSTISKAAEVVVRANGTGIIVAGQQVDNNLLACISSPIP 482

Query: 482 CILVDSDVGTKLYFYALHNS-NPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKP 541
           CILVD+ VG+KL+FY L NS +PVV LR ARTIIGKPI+  IA+FSSRGPNSVSP ILKP
Sbjct: 483 CILVDTIVGSKLFFYFLQNSDHPVVMLRAARTIIGKPIAPTIAHFSSRGPNSVSPPILKP 542

Query: 542 DIAAPGSNIVAAISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSAL 601
           DI+APGSNI++A+SPH  F+ KGF++ SGTSMA PH+S IVALLKS+HPTWSPAAIKSAL
Sbjct: 543 DISAPGSNILSAVSPHYFFNEKGFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKSAL 602

Query: 602 ITTASAKDPSGLPIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYI-YYLCA 661
           +TTA  +   GLPIFA+G+PPKVADPFDYG GVV+ NAA DPGLIYD+   DYI YYLC 
Sbjct: 603 MTTARTEVSPGLPIFADGTPPKVADPFDYGAGVVDANAAVDPGLIYDMGRKDYIDYYLCG 662

Query: 662 MGYQDSEISSITGQKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRA 721
           MGY+D +IS +T +K +CPLQ+ S+LDLNLP ITIP+L NST VTRTVTNVGNL+ VY+A
Sbjct: 663 MGYEDKDISHLTHRKTECPLQRLSLLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKA 722

Query: 722 VIDPPPGTKVSVEPSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPL 779
            I+ P G KVSV P  L F+SQVKKISFKV   T V RNYGYSFG LTWSDGVH+V+ PL
Sbjct: 723 KIEAPFGCKVSVNPQVLVFNSQVKKISFKVMFFTQVQRNYGYSFGRLTWSDGVHVVKIPL 760

BLAST of Spg021078 vs. NCBI nr
Match: KAG6577195.1 (Subtilisin-like protease 3.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 977.2 bits (2525), Expect = 8.0e-281
Identity = 517/785 (65.86%), Postives = 613/785 (78.09%), Query Frame = 0

Query: 1   MVNGQAP---LIVGVITIYALFSMFAYKPMAEADDQNRKVYIVYLGERQYEDSKLTTESH 60
           M N QAP   L+V V+TIY +FS      MAEADDQN KV+IVYLGER Y+D KLTT+SH
Sbjct: 1   MENNQAPSFMLVVAVVTIYVVFS-----AMAEADDQNSKVHIVYLGERPYDDVKLTTDSH 60

Query: 61  HDLLATVLGSKEKSSESMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQT 120
           H+LL +V+GSKEKS ESMVYSY+HGFSG AAKLT SQAQKLA MP V RV P+  YKM+T
Sbjct: 61  HELLESVMGSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSPLYKMRT 120

Query: 121 TRSWDFLGLSSSPS-NSNLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGR 180
           TRSWDFLGLSSSPS +SNLL R KMGD +IIGVID+G WPESE+F D G+GPIPSRWKG 
Sbjct: 121 TRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGI 180

Query: 181 CESGAEFNSTNCNRKIIGARWYSKGFIDNYGAEALANEYLSPRDANGHGTHVASTAAGSF 240
           C+SG +FNS++CN+K+IGARW++   + ++G EA+  +YLS RD  GHGTH ASTA G+F
Sbjct: 181 CQSGEDFNSSHCNKKVIGARWFASALVADHGEEAVFMDYLSARDNEGHGTHTASTAGGAF 240

Query: 241 VTNIGYHGLGIGTVRGGAPLARLAIYKVLWTNSGQGSIADILLAIDEAIHDGVDVLSMSI 300
           V N+ + G G GT+RGGAPLARLAIYKVLW++S  GS ADIL  IDEAIHDGVDVLSMSI
Sbjct: 241 VRNVSFFGNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSI 300

Query: 301 VGSIPSLPEFDELHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTM 360
             SIP   + +EL+  VA+G FHAIA+GISVVC+GGN+G +QQT+EN APW+ TVAAST+
Sbjct: 301 GKSIPLFSDVNELN-PVAVGSFHAIAKGISVVCSGGNEGSIQQTVENVAPWLFTVAASTI 360

Query: 361 DRAFLASI-ALGDNTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDGI 420
           DRAFLASI  LGDN TYLGQ+    KKD+VG L+                  + GRC G+
Sbjct: 361 DRAFLASITTLGDNATYLGQTFL--KKDIVGMLLA-----------------MDGRCAGL 420

Query: 421 FGNETFLSGKVV-LCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDNLLPFAIP 480
            G+   +SG VV LCF+D +A K     V   VM   +AK  GII AGQ ND L P   P
Sbjct: 421 LGSNIHISGNVVLLCFTD-LAKKAGASNV---VMPGKQAKVVGIIYAGQHNDILGPCDTP 480

Query: 481 CILVDSDVGTKLYFYALHNSNPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKPD 540
           CI VD+ VGTKL+ Y L++    +R+R  RTI GKPISS+IAYFSSRGPNS+SP ILKPD
Sbjct: 481 CIHVDTHVGTKLFTYYLNDEKAFIRVRATRTITGKPISSRIAYFSSRGPNSISPGILKPD 540

Query: 541 IAAPGSNIVAAISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSALI 600
           IAAPG+NI+AA+ P++    KGFA+ SGTSMAAPHISGIVAL+KSL PTWSPAAIKSALI
Sbjct: 541 IAAPGANIIAAVPPNHEGGDKGFAVMSGTSMAAPHISGIVALIKSLRPTWSPAAIKSALI 600

Query: 601 TTASAKDPSGLPIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYI-YYLCAM 660
           TTA  +D SGLPIFAEGSPPKVAD FDYGGGVV+ NAA DPGLIYDL+ TDYI YYLC+M
Sbjct: 601 TTARVEDLSGLPIFAEGSPPKVADSFDYGGGVVDANAAIDPGLIYDLEVTDYIYYYLCSM 660

Query: 661 GYQDSEISSITGQKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRAV 720
           GY +++IS ++ QK  CP ++ S+LDLNLPTIT+P LTNSTTVTRTVTNVGNLT+VY+AV
Sbjct: 661 GYTEADISHLSQQKTVCPSKRTSILDLNLPTITVPTLTNSTTVTRTVTNVGNLTAVYKAV 720

Query: 721 IDPPPGTKVSVEPSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPLS 779
           I  PPG+KV V+P  L F+S VKKISFKV  S+ + RNYGYSFG+LTW+DGVHLV+SPLS
Sbjct: 721 IKAPPGSKVRVKPRVLVFNSNVKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLS 756

BLAST of Spg021078 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 1.0e-222
Identity = 406/774 (52.45%), Postives = 542/774 (70.03%), Query Frame = 0

Query: 14  TIYALFSMFAYKPMAEADDQNRKVYIVYLGERQYEDSKLTTESHHDLLATVLGSKEKSSE 73
           ++  + S+     +A A  ++ KV+IVYLGE+Q++D K  TESHH +L+++LGSK+ + E
Sbjct: 7   SVLVVLSLIIVLNVARASAKS-KVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHE 66

Query: 74  SMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQTTRSWDFLGLSSSPSNS 133
           SMVYSYRHGFSG AAKLTKSQA+K+A+ P V+ V+P+S+Y++ TTR WD+LG  S+ ++ 
Sbjct: 67  SMVYSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYELATTRIWDYLG-PSADNSK 126

Query: 134 NLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGRCESGAEFNSTNCNRKII 193
           NL+    MGD  IIGVID+G+WPESE+F D G+GP+PS WKG CE G  F STNCNRK+I
Sbjct: 127 NLVSDTNMGDQTIIGVIDTGVWPESESFNDYGVGPVPSHWKGGCEPGENFISTNCNRKLI 186

Query: 194 GARWYSKGFI-DNYGAEALANEYLSPRDANGHGTHVASTAAGSFVTNIGYHGLGIGTVRG 253
           GA+++  GF+ +N      + +Y+S RD +GHGTHVAS A GSFV N+ Y GLG GT+RG
Sbjct: 187 GAKYFINGFLAENQFNATESPDYISARDFDGHGTHVASIAGGSFVPNVSYKGLGRGTLRG 246

Query: 254 GAPLARLAIYKVLW-TNSGQG---SIADILLAIDEAIHDGVDVLSMSIVGSIPSLPEFDE 313
           GAP AR+A+YK  W  N   G   S +DI+ AIDEAIHDGVDVLS+S+ G +P   E D 
Sbjct: 247 GAPRARIAMYKACWYINELDGVTCSFSDIMKAIDEAIHDGVDVLSISLGGRVPLNSETD- 306

Query: 314 LHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTMDRAFLASIALGD 373
           L  G+A G FHA+A+GI VVCAGGN GP  QT+ NTAPWILTVAA+T+DR+F   I LG+
Sbjct: 307 LRDGIATGAFHAVAKGIVVVCAGGNAGPSSQTVVNTAPWILTVAATTLDRSFATPIILGN 366

Query: 374 NTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDGI-FGNETFLSGKVV 433
           N   LGQ+++   +     LV P    +  ID         G C+ +   +   ++GKVV
Sbjct: 367 NQVILGQAMYIGPELGFTSLVYPEDPGN-SIDT------FSGVCESLNLNSNRTMAGKVV 426

Query: 434 LCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDNLLPFA--IPCILVDSDVGTK 493
           LCF+    T   F  V+ A   V  A G G+I+A     NL P +   PC+ +D+++GT 
Sbjct: 427 LCFT----TARDFTVVSTAASIVKAAGGLGLIIARNPGYNLAPCSDDFPCVAIDNELGTD 486

Query: 494 LYFYALHNSNPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKPDIAAPGSNIVAA 553
           + FY  +  +PVV+++ +RT++G+P+ +K+A FSSRGPNS+SPAILKPDIAAPG +I+AA
Sbjct: 487 ILFYIRYTGSPVVKIQPSRTLVGEPVGTKVATFSSRGPNSISPAILKPDIAAPGVSILAA 546

Query: 554 ISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSALITTASAKDPSGL 613
            SP+++ +  GF M+SGTSMAAP ISG++ALLKSLHP WSPAA +SA++TTA   DP G 
Sbjct: 547 TSPNDTLNAGGFVMRSGTSMAAPVISGVIALLKSLHPDWSPAAFRSAIVTTAWRTDPFGE 606

Query: 614 PIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYIYYLCAMGYQDSEISSITG 673
            I AE S  KV DPFDYGGG+VNP  AA+PGLI D+D+ DY+ YLC+ GY DS IS + G
Sbjct: 607 QIAAESSSLKVPDPFDYGGGLVNPEKAAEPGLILDMDSQDYVLYLCSAGYNDSSISRLVG 666

Query: 674 QKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRAVIDPPPGTKVSVE 733
           +   C   KPSVLD+NLP+ITIP L +  T+TRTVTNVG + SVY+ +++PP G +V V 
Sbjct: 667 KVTVCSNPKPSVLDINLPSITIPNLKDEVTLTRTVTNVGPVDSVYKVLVEPPLGIQVVVT 726

Query: 734 PSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPLSVRINFL 780
           P  L F+S+ K +SF V +ST    N G+ FG+LTW+D +H V  P+SVR   L
Sbjct: 727 PETLVFNSKTKSVSFTVIVSTTHKINTGFYFGSLTWTDSIHNVVIPVSVRTQIL 766

BLAST of Spg021078 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 7.4e-221
Identity = 402/755 (53.25%), Postives = 531/755 (70.33%), Query Frame = 0

Query: 36  KVYIVYLGERQYEDSKLTTESHHDLLATVLGSKEKSSESMVYSYRHGFSGLAAKLTKSQA 95
           KV+IVYLGE+Q++D +  TESHH +L ++LGSKE +  SMV+SYRHGFSG AAKLTKSQA
Sbjct: 29  KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQA 88

Query: 96  QKLAEMPGVVRVLPNSFYKMQTTRSWDFLGLSSSPSNSNLLRRGKMGDGIIIGVIDSGIW 155
           +KLA++P VV V P+SFY++ TTR+WD+LGLS + +  NLL    MG+ +IIG++DSG+W
Sbjct: 89  KKLADLPEVVHVTPDSFYQLDTTRTWDYLGLSVA-NPKNLLNDTNMGEEVIIGIVDSGVW 148

Query: 156 PESEAFRDNGLGPIPSRWKGRCESGAEFNSTNCNRKIIGARWYSKGFIDNY----GAEAL 215
           PESE F DNG+GP+PS WKG C SG  F S+ CN+K+IGA+++  GF+  +      E+L
Sbjct: 149 PESEVFNDNGIGPVPSHWKGGCVSGENFTSSQCNKKLIGAKYFINGFLATHESFNSTESL 208

Query: 216 ANEYLSPRDANGHGTHVASTAAGSFVTNIGYHGLGIGTVRGGAPLARLAIYKVLW----T 275
             +++SPRD +GHGTHVA+ A GS+V +I Y GL  GTVRGGAP AR+A+YK  W     
Sbjct: 209 --DFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRARIAMYKACWYLDRF 268

Query: 276 NSGQGSIADILLAIDEAIHDGVDVLSMSIVGSIPSLPEFDELHGGVAIGLFHAIARGISV 335
           +    S ADIL A+DEA+HDGVDVLS+SI    P  PE D +   +A G FHA+ +GI+V
Sbjct: 269 DINTCSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETD-VRAVIATGAFHAVLKGITV 328

Query: 336 VCAGGNDGPLQQTMENTAPWILTVAASTMDRAFLASIALGDNTTYLGQSLFSAKKDVVGQ 395
           VC+GGN GP  QT+ NTAPWILTVAA+T+DR+F   I LG+N   LGQ++++  +     
Sbjct: 329 VCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNKLILGQAMYTGPELGFTS 388

Query: 396 LVLPPTTRSLHIDVLYWIYYIGGRCDGIFGNETF-LSGKVVLCFSDGVATKTIFETVAVA 455
           LV P    + +           G C+ +F N    ++GKVVLCF+    T T + TV+ A
Sbjct: 389 LVYPENPGNSNES-------FSGDCELLFFNSNHTMAGKVVLCFT----TSTRYITVSSA 448

Query: 456 VMAVAKAKGRGIIVAGQQNDNLLPFA--IPCILVDSDVGTKLYFYALHNSNPVVRLRQAR 515
           V  V +A G G+IVA    DNL P     PC+ VD ++GT +  Y      PVV+++ ++
Sbjct: 449 VSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTDILLYIRSTGLPVVKIQPSK 508

Query: 516 TIIGKPISSKIAYFSSRGPNSVSPAILKPDIAAPGSNIVAAISPHNSFSYKGFAMQSGTS 575
           T++G+P+ +K+A FSSRGPNS+ PAILKPDIAAPG +I+AA + + +F+ +GF   SGTS
Sbjct: 509 TLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAATTTNKTFNDRGFIFLSGTS 568

Query: 576 MAAPHISGIVALLKSLHPTWSPAAIKSALITTASAKDPSGLPIFAEGSPPKVADPFDYGG 635
           MAAP ISG+VALLK+LH  WSPAAI+SA++TTA   DP G  IFAEGSP K+ADPFDYGG
Sbjct: 569 MAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKLADPFDYGG 628

Query: 636 GVVNPNAAADPGLIYDLDTTDYIYYLCAMGYQDSEISSITGQKRKCPLQKPSVLDLNLPT 695
           G+VNP  AA PGL+YDL   DY+ Y+C++GY ++ IS + G+   C   KPSVLD NLP+
Sbjct: 629 GLVNPEKAAKPGLVYDLGLEDYVLYMCSVGYNETSISQLVGKGTVCSNPKPSVLDFNLPS 688

Query: 696 ITIPALTNSTTVTRTVTNVGNLTSVYRAVIDPPPGTKVSVEPSKLAFSSQVKKISFKVTI 755
           ITIP L +  T+TRT+TNVG L SVY+ VI+PP G +V+V P  L F+S  K++SFKV +
Sbjct: 689 ITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTTKRVSFKVKV 748

Query: 756 STAVYRNYGYSFGTLTWSDGVHLVQSPLSVRINFL 780
           ST    N GY FG+LTWSD +H V  PLSVR   L
Sbjct: 749 STTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQIL 768

BLAST of Spg021078 vs. ExPASy Swiss-Prot
Match: Q9SZY2 (Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 SV=2)

HSP 1 Score: 763.8 bits (1971), Expect = 1.8e-219
Identity = 406/790 (51.39%), Postives = 545/790 (68.99%), Query Frame = 0

Query: 1   MVNGQAPLIVGVITIYALFSMFAYKPMAEADDQNRKVYIVYLGERQYEDSKLTTESHHDL 60
           M N +  + V +  +  L     + P A A+    KV+IVYLGE+Q++D +  TESHH +
Sbjct: 1   MRNHRTSIFVVLSLVIILNGQSGFLPRAGAES---KVHIVYLGEKQHDDPEFVTESHHRM 60

Query: 61  LATVLGSKEKSSESMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQTTRS 120
           L ++LGSKE++  SMV+S+RHGFSG AAKLT+SQA+K+A++P VV V+P+ FYK  TTR+
Sbjct: 61  LWSLLGSKEEAHGSMVHSFRHGFSGFAAKLTESQAKKIADLPEVVHVIPDRFYKPATTRT 120

Query: 121 WDFLGLSSSPSN-SNLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGRCES 180
           WD+LGL  SP+N  NLL +  MG+ +IIG+IDSG+WPESE F DN +GP+PS WKG CES
Sbjct: 121 WDYLGL--SPTNPKNLLNQTNMGEQMIIGIIDSGVWPESEVFNDNEIGPVPSHWKGGCES 180

Query: 181 GAEFNSTNCNRKIIGARWYSKGFIDNY----GAEALANEYLSPRDANGHGTHVASTAAGS 240
           G +FNS++CN+K+IGA+++   F+  +     +E+L  +++SPR  NGHGTHVA+ A GS
Sbjct: 181 GEDFNSSHCNKKLIGAKYFINAFLATHESFNSSESL--DFISPRGYNGHGTHVATIAGGS 240

Query: 241 FVTNIGYHGLGIGTVRGGAPLARLAIYKVLW---TNSGQGSIADILLAIDEAIHDGVDVL 300
           +V N  Y GL  GTVRGGAP AR+A+YK  W    +    S ADIL A+DEAIHDGVDVL
Sbjct: 241 YVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLDLDIAACSSADILKAMDEAIHDGVDVL 300

Query: 301 SMSIVGSIPSLPEFDELHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVA 360
           S+S+ G  P  PE D +  G+A G FHA+ +GI+VVCA GN GP  QT+ NTAPWILTVA
Sbjct: 301 SLSL-GFEPLYPETD-VRDGIATGAFHAVLKGITVVCAAGNAGPAAQTVGNTAPWILTVA 360

Query: 361 ASTMDRAFLASIALGDNTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRC 420
           A+T+DR+F+  + LG+N   LGQ++++  +     LV P    + +           G C
Sbjct: 361 ATTLDRSFVTPMTLGNNKVILGQAIYTGTEVGFTSLVYPENPGNSNES-------FSGTC 420

Query: 421 DGIFGNET-FLSGKVVLCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDNLLPF 480
           + +  N    ++GKVVLCF++   +     +V  A   V +A G G+I+AGQ  + L P 
Sbjct: 421 ERLLINSNRTMAGKVVLCFTESPYS----ISVTRAAHYVKRAGGLGVIIAGQPGNVLRPC 480

Query: 481 A--IPCILVDSDVGTKLYFYALHNSNPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPA 540
               PC+ VD ++GT + FY   N +PVV+++ +RT+IG+P+ +K+A FSSRGPN +S A
Sbjct: 481 LDDFPCVAVDYELGTYILFYIRSNGSPVVKIQPSRTLIGQPVGTKVASFSSRGPNPISAA 540

Query: 541 ILKPDIAAPGSNIVAAISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAI 600
           ILKPDIAAPG +I+AA + + +F+ +GF   SGTSMA P ISGIVALLK+LHP WSPAAI
Sbjct: 541 ILKPDIAAPGVSILAATTTNTTFNDRGFIFLSGTSMATPTISGIVALLKALHPDWSPAAI 600

Query: 601 KSALITTASAKDPSGLPIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYIYY 660
           +SA++TTA   DP G  IFAEGSP K ADPFDYGGG+VNP  A  PGL+YDL   DY+ Y
Sbjct: 601 RSAIVTTAWRTDPFGEQIFAEGSPRKPADPFDYGGGLVNPEKATKPGLVYDLGLEDYVLY 660

Query: 661 LCAMGYQDSEISSITGQKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSV 720
           +C++GY ++ IS + G+   C   KPSVLD NLP+ITIP L    T+ RT+TNVG L SV
Sbjct: 661 MCSVGYNETSISQLVGKGTVCSYPKPSVLDFNLPSITIPNLKEEVTLPRTLTNVGPLESV 720

Query: 721 YRAVIDPPPGTKVSVEPSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQ 780
           YR  ++PP GT+V+V P  L F+S  K++SFKV++ST    N GY FG+LTWSD +H V 
Sbjct: 721 YRVAVEPPLGTQVTVTPETLVFNSTTKRVSFKVSVSTTHKINTGYYFGSLTWSDSLHNVT 770

BLAST of Spg021078 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 3.1e-219
Identity = 396/779 (50.83%), Postives = 537/779 (68.93%), Query Frame = 0

Query: 14  TIYALFSMFAY----KPMAEADDQNRKVYIVYLGERQYEDSKLTTESHHDLLATVLGSKE 73
           +IY + S+  +    +         RKV+IVYLGE+Q++D +  TESHH +L ++LGSKE
Sbjct: 7   SIYVVLSLVIFLNVQRSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKE 66

Query: 74  KSSESMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQTTRSWDFLGLSSS 133
            +++SMVYSYRHGFSG AAKLT+SQA+K+A++P VV V+P+SFYK+ TTR+WD+LGLS++
Sbjct: 67  DANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAA 126

Query: 134 PSNSNLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGRCESGAEFNSTNCN 193
              S LL    MG+ IIIGVID+G+WPESE F D+G GP+PS WKG CE+G  FNS+NCN
Sbjct: 127 NPKS-LLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCN 186

Query: 194 RKIIGARWYSKGFIDNYGAEALAN--EYLSPRDANGHGTHVASTAAGSFVTNIGYHGLGI 253
           +K+IGA+++  GF+    +    N  +++SPRD +GHGTHV++ A GSFV NI Y GL  
Sbjct: 187 KKLIGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAG 246

Query: 254 GTVRGGAPLARLAIYKVLW----TNSGQGSIADILLAIDEAIHDGVDVLSMSIVGSIPSL 313
           GTVRGGAP A +A+YK  W     ++   S ADIL A+DEA+HDGVDVLS+S+  S+P  
Sbjct: 247 GTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLY 306

Query: 314 PEFDELHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTMDRAFLAS 373
            E D +  G+  G FHA+ +GI+VVC+GGN GP   T+ NTAPWI+TVAA+T+DR+F   
Sbjct: 307 GETD-IRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATP 366

Query: 374 IALGDNTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDG-IFGNETFL 433
           + LG+N   LGQ++++        LV P    + +           G C+  +F +   +
Sbjct: 367 LTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNES-------FSGTCEELLFNSNRTM 426

Query: 434 SGKVVLCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDNLLPFA--IPCILVDS 493
            GKVVLCF+    T      V  A   V +A G G+I+A      + P     PC+ VD 
Sbjct: 427 EGKVVLCFT----TSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDW 486

Query: 494 DVGTKLYFYALHNSNPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKPDIAAPGS 553
           ++GT +  Y   + +PVV+++ ++T++G+P+ +K+A FSSRGPNS++PAILKPDIAAPG 
Sbjct: 487 ELGTDILLYTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGV 546

Query: 554 NIVAAISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSALITTASAK 613
           +I+AA + + +FS +GF M SGTSMAAP ISG+ ALLK+LH  WSPAAI+SA++TTA   
Sbjct: 547 SILAA-TTNTTFSDQGFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKT 606

Query: 614 DPSGLPIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYIYYLCAMGYQDSEI 673
           DP G  IFAEGSPPK+ADPFDYGGG+VNP  +A+PGL+YD+   DY+ Y+C++GY ++ I
Sbjct: 607 DPFGEQIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSI 666

Query: 674 SSITGQKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRAVIDPPPGT 733
           S + G+   C   KPSVLD NLP+ITIP L +  T+TRTVTNVG L SVYR  ++PP G 
Sbjct: 667 SQLIGKTTVCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGF 726

Query: 734 KVSVEPSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPLSVRINFL 780
           +V+V P  L F+S  KK+ FKV +ST    N GY FG+LTWSD +H V  PLSVR   L
Sbjct: 727 QVTVTPETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQIL 771

BLAST of Spg021078 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 9.1e-219
Identity = 404/776 (52.06%), Postives = 532/776 (68.56%), Query Frame = 0

Query: 13  ITIYALFSMFAYKPMAEADDQNRKVYIVYLGERQYEDSKLTTESHHDLLATVLGSKEKSS 72
           + +  + S+     +  A D++ KV+IVYLGE+Q++D +  +ESHH +L+++LGSK  + 
Sbjct: 6   VLLVLVLSLVIVLNVVRASDES-KVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAH 65

Query: 73  ESMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQTTRSWDFLGLSSSPSN 132
           ESMVYSYRHGFSG AAKLT+SQA+KLA+ P VV V+ +SFY++ TTR+WD+LGLS +  N
Sbjct: 66  ESMVYSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGLSVANPN 125

Query: 133 SNLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGRCESGAEFNSTNCNRKI 192
            NLL    MGD +IIG ID+G+WPESE+F DNG+GPIPS WKG CESG +F STNCNRK+
Sbjct: 126 -NLLNDTNMGDQVIIGFIDTGVWPESESFNDNGVGPIPSHWKGGCESGEKFISTNCNRKL 185

Query: 193 IGARWYSKGFI-DNYGAEAL-ANEYLSPRDANGHGTHVASTAAGSFVTNIGYHGLGIGTV 252
           IGA+++  GF+ +N G     + +Y+S RD  GHGTH AS A GSFV NI Y GL  G +
Sbjct: 186 IGAKYFINGFLAENEGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYKGLAGGNL 245

Query: 253 RGGAPLARLAIYKVLWTNSGQGSIA----DILLAIDEAIHDGVDVLSMSIVGSIPSLPEF 312
           RGGAP AR+AIYK  W     G++A    DIL A+DE++HDGVDVLS+S+   IP  PE 
Sbjct: 246 RGGAPRARIAIYKACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPET 305

Query: 313 DELHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTMDRAFLASIAL 372
           D L   +A G FHA+A+GI VVCAGGN GP  QT+ NTAPWI+TVAA+T+DR+F   I L
Sbjct: 306 D-LRDRIATGAFHAVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPITL 365

Query: 373 GDNTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDGIFGN-ETFLSGK 432
           G+    LGQ+L++ ++     LV P           +      G C+ +  N    ++GK
Sbjct: 366 GNRKVILGQALYTGQELGFTSLVYPENAG-------FTNETFSGVCERLNLNPNRTMAGK 425

Query: 433 VVLCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDNLLPFA--IPCILVDSDVG 492
           VVLCF+    T T+F  V+ A   V  A G G+I+A     NL P     PC+ +D ++G
Sbjct: 426 VVLCFT----TNTLFTAVSRAASYVKAAGGLGVIIARNPGYNLTPCRDDFPCVAIDYELG 485

Query: 493 TKLYFYALHNSNPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKPDIAAPGSNIV 552
           T +  Y     +PVV+++ +RT++G+P+ +K+A FSSRGPNS+SPAILKPDI APG +I+
Sbjct: 486 TDVLLYIRSTRSPVVKIQPSRTLVGQPVGTKVATFSSRGPNSISPAILKPDIGAPGVSIL 545

Query: 553 AAISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSALITTASAKDPS 612
           AA SP ++ S  GF + +GTSMAAP ++G+VALLK+LHP WSPAA +SA++TTA   DP 
Sbjct: 546 AATSPDSNSSVGGFDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRSAIVTTAWRTDPF 605

Query: 613 GLPIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYIYYLCAMGYQDSEISSI 672
           G  IFAEGS  KVADPFDYGGG+VNP  AADPGLIYD+   DYI YLC+ GY DS I+ +
Sbjct: 606 GEQIFAEGSSRKVADPFDYGGGIVNPEKAADPGLIYDMGPRDYILYLCSAGYNDSSITQL 665

Query: 673 TGQKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRAVIDPPPGTKVS 732
            G    C   K SVLD+NLP+ITIP L +  T+TRTVTNVG + SVY+ V++PP G +V 
Sbjct: 666 VGNVTVCSTPKTSVLDVNLPSITIPDLKDEVTLTRTVTNVGTVDSVYKVVVEPPLGIQVV 725

Query: 733 VEPSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPLSVRINFL 780
           V P  L F+S+ K +SF V +ST    N G+ FG L W+D +H V  P+SVR   L
Sbjct: 726 VAPETLVFNSKTKNVSFTVRVSTTHKINTGFYFGNLIWTDSMHNVTIPVSVRTQIL 767

BLAST of Spg021078 vs. ExPASy TrEMBL
Match: A0A6J1JBL4 (subtilisin-like protease SBT3.9 OS=Cucurbita maxima OX=3661 GN=LOC111482985 PE=3 SV=1)

HSP 1 Score: 995.0 bits (2571), Expect = 1.8e-286
Identity = 522/783 (66.67%), Postives = 615/783 (78.54%), Query Frame = 0

Query: 1   MVNGQAP-LIVGVITIYALFSMFAYKPMAEADDQNRKVYIVYLGERQYEDSKLTTESHHD 60
           M N +AP  I+ V+TIYA+FS      MAEADDQN KV+IVYLGER Y+D KLTT+SHH+
Sbjct: 1   MENNRAPSFILAVVTIYAVFS-----DMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHE 60

Query: 61  LLATVLGSKEKSSESMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQTTR 120
           LL +VLGSKEKS ESMVYSYRHGFSG AAKLT SQAQKLA MP V RV P+S YKM+TTR
Sbjct: 61  LLESVLGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTR 120

Query: 121 SWDFLGLSSSPS-NSNLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGRCE 180
           SWDFLGLSSSPS +SNL  R KMGD +IIGVID+G WPESE+F D G+GPIPSRWKG C+
Sbjct: 121 SWDFLGLSSSPSESSNLFHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQ 180

Query: 181 SGAEFNSTNCNRKIIGARWYSKGFIDNYGAEALANEYLSPRDANGHGTHVASTAAGSFVT 240
           SG +FNS++CN+K+IGARW++   I ++G EA+  +YLS RD  GHGTH ASTA G+FV 
Sbjct: 181 SGEDFNSSHCNKKVIGARWFASALIADHGEEAVFKDYLSARDNEGHGTHTASTAGGAFVR 240

Query: 241 NIGYHGLGIGTVRGGAPLARLAIYKVLWTNSGQGSIADILLAIDEAIHDGVDVLSMSIVG 300
           N+ Y G G GT+RGGAPLARLAIYKVLW++S  GS ADIL  IDEAIHDGVDVLSMSI  
Sbjct: 241 NVSYFGNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIGK 300

Query: 301 SIPSLPEFDELHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTMDR 360
           SIP  P+ ++++  VA+G FHAIA+GISVVCAGGN+G +QQT+EN APW+ TVAAST+DR
Sbjct: 301 SIPLFPDVNDVN-PVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDR 360

Query: 361 AFLASI-ALGDNTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDGIFG 420
           AFL SI  LGDN TYLGQ     +KD+VG LV                  + GRC GI G
Sbjct: 361 AFLVSITTLGDNATYLGQMFL--RKDIVGMLVA-----------------MDGRCAGILG 420

Query: 421 NETFLSGKVV-LCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDNLLPFAIPCI 480
            +  +SG VV LCF+D   T       + AVM   +AK  G+I AGQQ DNL+P  IPCI
Sbjct: 421 GDIPISGNVVLLCFTDLAKT----APASNAVMPGKQAKVVGVIYAGQQTDNLVPCDIPCI 480

Query: 481 LVDSDVGTKLYFYALHNSNPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKPDIA 540
            VD+ VGTKL+ Y L++ + ++RLR  RTIIGKPISS+IAYFSSRGPNS SP ILKPDIA
Sbjct: 481 HVDTHVGTKLFTYLLNDDDALIRLRATRTIIGKPISSRIAYFSSRGPNSFSPGILKPDIA 540

Query: 541 APGSNIVAAISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSALITT 600
           APG+NI+AA+ P++    KGFA  SGTSMA PHISGIV L+KSLHPTWSPAAIKSALITT
Sbjct: 541 APGANIIAAVPPNHKGGDKGFATMSGTSMATPHISGIVTLIKSLHPTWSPAAIKSALITT 600

Query: 601 ASAKDPSGLPIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYI-YYLCAMGY 660
           A  +DPSG+PIFAEGSPPKVADPFDYGGGVV+ NAA DPGLIYDL  TDYI YYLC+MGY
Sbjct: 601 ARVEDPSGMPIFAEGSPPKVADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGY 660

Query: 661 QDSEISSITGQKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRAVID 720
            +++IS ++ QK  CP ++ S+LDLNLPTIT+PAL NSTTVTRTVTNVGNLT+VY+AVI 
Sbjct: 661 TEADISHLSQQKTVCPSKRASILDLNLPTITVPALINSTTVTRTVTNVGNLTAVYKAVIK 720

Query: 721 PPPGTKVSVEPSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPLSVR 779
            PPG+KV V+P  L F+S VKKISFKV  S+ + RNYGYSFG+LTW+DGVHLV+SPLSVR
Sbjct: 721 APPGSKVRVKPRVLVFNSNVKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVR 754

BLAST of Spg021078 vs. ExPASy TrEMBL
Match: A0A0A0KWS6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G047950 PE=3 SV=1)

HSP 1 Score: 995.0 bits (2571), Expect = 1.8e-286
Identity = 523/780 (67.05%), Postives = 611/780 (78.33%), Query Frame = 0

Query: 8   LIVGVITIYALFSMFAYKP-MAEADDQNRKVYIVYLGERQYEDSKLTTESHHDLLATVLG 67
           LIVG I    LF     KP +AEADDQN KV+IVYLGE+ + D+K T +SHH LL+T+LG
Sbjct: 10  LIVGFIIFDCLF-----KPILAEADDQNPKVHIVYLGEKPHHDTKFTIDSHHQLLSTILG 69

Query: 68  SKEKSSESMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQTTRSWDFLGL 127
           SKEKS E+MVYSY+HGFSG AAKLTKSQAQKL+EM  VVRV+P+S YK+ TTRSWDFLGL
Sbjct: 70  SKEKSMEAMVYSYKHGFSGFAAKLTKSQAQKLSEMSRVVRVVPSSLYKVHTTRSWDFLGL 129

Query: 128 SSSP-SNSNLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGRCESGAEFNS 187
           SSSP  +SNLL R +MG+ +IIGVID+GIWPESE+F+D G+G IPSRWKG CESG +FNS
Sbjct: 130 SSSPFESSNLLHRAQMGENVIIGVIDTGIWPESESFKDKGVGSIPSRWKGTCESGEQFNS 189

Query: 188 TNCNRKIIGARWYSKGFIDNYGAEALANEYLSPRDANGHGTHVASTAAGSFVTNIGYHGL 247
           TNCN+KIIGARW+ KGF+ + G +ALA EYLSPRD NGHGTH AS AAGSFV NI YH  
Sbjct: 190 TNCNKKIIGARWFMKGFVADLGRDALAKEYLSPRDLNGHGTHTASIAAGSFVANINYHNN 249

Query: 248 GIGTVRGGAPLARLAIYKVLWTNSGQGSIADILLAIDEAIHDGVDVLSMSIVGSIPSLPE 307
             GTVRGGAPLARLAIYK LWT    GS ADIL AIDEAI+DGVDVLSMSI    P LPE
Sbjct: 250 AAGTVRGGAPLARLAIYKALWTKDAVGSTADILKAIDEAINDGVDVLSMSIGSLTPFLPE 309

Query: 308 FDELHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTMDRAFLASI- 367
           F+E +  +A G FHAIA+GISVVCA GN GP  QT+EN APWI TVAA+T+DRAFLASI 
Sbjct: 310 FNEAN-DIAFGSFHAIAKGISVVCAAGNSGPTPQTVENVAPWIFTVAANTIDRAFLASIT 369

Query: 368 ALGDNTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDGIFGNETFLSG 427
            L DNTT+LGQSL  +KKD+V +L    T                GRCD + GNETF++G
Sbjct: 370 TLPDNTTFLGQSLLDSKKDLVAELETLDT----------------GRCDDLLGNETFING 429

Query: 428 KVVLCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDNL---LPFAIPCILVDSD 487
           KVV+CFS+     TI++    A MAVA+A G GIIVAGQQ+D+L   +P  IPCILVD+D
Sbjct: 430 KVVMCFSNLADHNTIYD----AAMAVARANGTGIIVAGQQDDDLFSCIPSPIPCILVDTD 489

Query: 488 VGTKLYFYAL--HNSNPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKPDIAAPG 547
           VG+KL+F  L  +++NPVVRLR  RTIIGKPI+  I+YFSSRGPNSVS  ILKPDI+APG
Sbjct: 490 VGSKLFFINLLQNSTNPVVRLRATRTIIGKPITPAISYFSSRGPNSVSNPILKPDISAPG 549

Query: 548 SNIVAAISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSALITTASA 607
           SNI+AA+SPH+ F+ KGF + SGTSMA PHIS IVALLKS+HPTWSPAAIKSAL+TTA  
Sbjct: 550 SNILAAVSPHHIFNEKGFMLLSGTSMATPHISAIVALLKSVHPTWSPAAIKSALMTTART 609

Query: 608 KDPSGLPIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYI-YYLCAMGYQDS 667
           +   GLPIFAEG+PPK+ADPFDYGGG+V+ NAA DPGL+YD+   DYI YYLC MGY+D 
Sbjct: 610 EVSPGLPIFAEGTPPKMADPFDYGGGIVDANAAVDPGLVYDMGRKDYIDYYLCGMGYKDE 669

Query: 668 EISSITGQKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRAVIDPPP 727
           +IS +T +K  CPLQ+ SVLDLNLP ITIP+L NST VTRTVTNVGNL+ VY+A I+ P 
Sbjct: 670 DISHLTQRKTVCPLQRLSVLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAEIESPF 729

Query: 728 GTKVSVEPSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPLSVRINF 779
           G KVSV P  L F+SQVKKISFKV   T V RNYGYSFG LTW+DG+H+V+ PLSVR  F
Sbjct: 730 GCKVSVNPQVLVFNSQVKKISFKVMFFTQVQRNYGYSFGRLTWTDGIHVVKIPLSVRFGF 763

BLAST of Spg021078 vs. ExPASy TrEMBL
Match: A0A1S3BU23 (subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493167 PE=3 SV=1)

HSP 1 Score: 978.0 bits (2527), Expect = 2.3e-281
Identity = 521/786 (66.28%), Postives = 604/786 (76.84%), Query Frame = 0

Query: 2   VNGQ-APLIVGVITIYALFSMFAYKPMAEADDQNRKVYIVYLGERQYEDSKLTTESHHDL 61
           +N Q A LIVG I  Y L        +AE++DQN KV+IVYLGE+ + D+K TT+SHH L
Sbjct: 3   INSQLAHLIVGFIIFYGLCEPI----LAESNDQNPKVHIVYLGEKPHHDTKFTTDSHHQL 62

Query: 62  LATVLGSKEKSSESMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQTTRS 121
           LA +LGSKEKS E+MVYSY+HGFSG AAKLTKS+AQKL+EM  VVRV+P+S YK+ TTRS
Sbjct: 63  LAAILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEAQKLSEMSRVVRVVPSSLYKVHTTRS 122

Query: 122 WDFLGLSSSPS-NSNLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGRCES 181
           WDFLGLSSSPS +SNLL R K GD +IIGVIDSGIWPESE+F+D GLGPIPSRWKG CES
Sbjct: 123 WDFLGLSSSPSESSNLLHRAK-GDDVIIGVIDSGIWPESESFKDKGLGPIPSRWKGTCES 182

Query: 182 GAEFNSTNCNRKIIGARWYSKGFIDNYGAEALANEYLSPRDANGHGTHVASTAAGSFVTN 241
           G +FNSTNCN+KIIGARW+ K F+ +YG EALA EYLSPRD +GHGTH ASTAAGSFV N
Sbjct: 183 GEQFNSTNCNKKIIGARWFVKAFVADYGREALAKEYLSPRDLHGHGTHTASTAAGSFVAN 242

Query: 242 IGYHGLGIGTVRGGAPLARLAIYKVLWTNSGQGSIADILLAIDEAIHDGVDVLSMSIVGS 301
           I YH    GT RGGAPLARLAIYK LWTN G GS ADIL AIDEAIHDGVDVLS+SI GS
Sbjct: 243 INYHNNAAGTARGGAPLARLAIYKALWTNRGVGSSADILKAIDEAIHDGVDVLSISIGGS 302

Query: 302 IPSLPEFDELHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTMDRA 361
            P  PEF EL   +A G FHAI +GISVVCA GN GP  Q ++N APWI TVA +T+DRA
Sbjct: 303 PPFYPEFTEL-SDIAFGSFHAITKGISVVCAAGNSGPSPQMVDNVAPWIFTVATNTIDRA 362

Query: 362 FLASI-ALGDNTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDGIFGN 421
           FL+SI  L DNTT++GQSL  +KKD+V +LV              W      RCD + GN
Sbjct: 363 FLSSITTLRDNTTFMGQSLLESKKDLVAELV-------------SW-----ERCDQLSGN 422

Query: 422 ETFLSGKVVLCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDN----LLPFAIP 481
           E F++GKVVLCF         + T++ A   V +A G GIIVAGQQ DN     +   IP
Sbjct: 423 EAFINGKVVLCF----PKLADYSTISKAAEVVVRANGTGIIVAGQQVDNNLLACISSPIP 482

Query: 482 CILVDSDVGTKLYFYALHNS-NPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKP 541
           CILVD+ VG+KL+FY L NS +PVV LR ARTIIGKPI+  IA+FSSRGPNSVSP ILKP
Sbjct: 483 CILVDTIVGSKLFFYFLQNSDHPVVMLRAARTIIGKPIAPTIAHFSSRGPNSVSPPILKP 542

Query: 542 DIAAPGSNIVAAISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSAL 601
           DI+APGSNI++A+SPH  F+ KGF++ SGTSMA PH+S IVALLKS+HPTWSPAAIKSAL
Sbjct: 543 DISAPGSNILSAVSPHYFFNEKGFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKSAL 602

Query: 602 ITTASAKDPSGLPIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYI-YYLCA 661
           +TTA  +   GLPIFA+G+PPKVADPFDYG GVV+ NAA DPGLIYD+   DYI YYLC 
Sbjct: 603 MTTARTEVSPGLPIFADGTPPKVADPFDYGAGVVDANAAVDPGLIYDMGRKDYIDYYLCG 662

Query: 662 MGYQDSEISSITGQKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRA 721
           MGY+D +IS +T +K +CPLQ+ S+LDLNLP ITIP+L NST VTRTVTNVGNL+ VY+A
Sbjct: 663 MGYEDKDISHLTHRKTECPLQRLSLLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKA 722

Query: 722 VIDPPPGTKVSVEPSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPL 779
            I+ P G KVSV P  L F+SQVKKISFKV   T V RNYGYSFG LTWSDGVH+V+ PL
Sbjct: 723 KIEAPFGCKVSVNPQVLVFNSQVKKISFKVMFFTQVQRNYGYSFGRLTWSDGVHVVKIPL 760

BLAST of Spg021078 vs. ExPASy TrEMBL
Match: A0A6J1EZK4 (subtilisin-like protease SBT3.7 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 4.8e-279
Identity = 520/785 (66.24%), Postives = 602/785 (76.69%), Query Frame = 0

Query: 1   MVNGQAP---LIVGVITIYALFSMFAYKPMAEADDQNRKVYIVYLGERQYEDSKLTTESH 60
           M N QAP   L V ++TIYA+FS      MAE DDQN KV+IVYLGER Y+D KLTT SH
Sbjct: 1   MENNQAPSFMLAVAIVTIYAVFS-----AMAEVDDQNSKVHIVYLGERPYDDVKLTTHSH 60

Query: 61  HDLLATVLGSKEKSSESMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQT 120
           H+LL +VL SKEKS ESMVYSY+HGFSG AAKLT SQAQKLA MP V RV P+S YKM T
Sbjct: 61  HELLESVLRSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHT 120

Query: 121 TRSWDFLGLSSSPS-NSNLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGR 180
           TRSWDFLGLSSSPS +SNLL R KMGD +IIGVIDSG WPESE+F D G+GPIPSRWKG 
Sbjct: 121 TRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGI 180

Query: 181 CESGAEFNSTNCNRKIIGARWYSKGFIDNYGAEALANEYLSPRDANGHGTHVASTAAGSF 240
           C+ G +FNS +CN+K+IGARW+++  I ++G EA+  +YLS RD  GHGTH ASTA G+F
Sbjct: 181 CQLGEDFNSFHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAF 240

Query: 241 VTNIGYHGLGIGTVRGGAPLARLAIYKVLWTNSGQGSIADILLAIDEAIHDGVDVLSMSI 300
           V N+ Y G   GT+RGGAPLARLAIYKV+W++   GS ADIL  IDEAIHDGVDVLSMSI
Sbjct: 241 VRNVSYFGNRRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSI 300

Query: 301 VGSIPSLPEFDELHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTM 360
            GSIP  P+  E +  VAIG FHAIARG+SVVCAGGN+G +QQT+ N APW+ TVAAST+
Sbjct: 301 GGSIPLPPDVSEWN-TVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTI 360

Query: 361 DRAFLASI-ALGDNTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDGI 420
           DRAFL SI  LGDN TYLGQ+    KKDVVG+LV+                 +  RC G 
Sbjct: 361 DRAFLTSITTLGDNATYLGQTFL--KKDVVGKLVV-----------------MDRRCAGH 420

Query: 421 FGNETFLSGKVV-LCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDNLLPFAIP 480
            G++    G VV LCF D +A K      +  VM V +AK  G+I AGQ  D L P  +P
Sbjct: 421 LGSDIPTGGNVVLLCFID-LAMKA---AASNPVMPVKQAKVVGVIYAGQHTDILGPCDVP 480

Query: 481 CILVDSDVGTKLYFYALHNSNPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKPD 540
           CI VD+ VGTKL  Y L +   ++RL+  RTI+GKPISS+IAYFSSRGPNSVSP ILKPD
Sbjct: 481 CIHVDTHVGTKLLTYILSDEKALIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPD 540

Query: 541 IAAPGSNIVAAISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSALI 600
           IAAPGSNI+AA+ P++    KGFA  SGTSMA PHISGIVAL+KSL PTWSPAAIKSALI
Sbjct: 541 IAAPGSNIIAAVPPNHKGGDKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALI 600

Query: 601 TTASAKDPSGLPIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYI-YYLCAM 660
           TTA  +DPSGLPIFAEGSPPKVADPFDYGGGVV+ NAA DPGLIYDL  TDYI YY+C+M
Sbjct: 601 TTARVEDPSGLPIFAEGSPPKVADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSM 660

Query: 661 GYQDSEISSITGQKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRAV 720
           GY  +EIS ++ QK  CP ++ SVLDLNLPTIT+PALTNSTTVTRTVTNVGNLT+VY+AV
Sbjct: 661 GYTKAEISHLSQQKIVCPSKRASVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAV 720

Query: 721 IDPPPGTKVSVEPSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPLS 779
           I  PPG+KV V P  L F+S VKKISFKV  S+ + RNYGYSFG+LTW+DGVHLV+SPLS
Sbjct: 721 IKAPPGSKVRVNPRVLVFNSNVKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLS 756

BLAST of Spg021078 vs. ExPASy TrEMBL
Match: A0A6J1EZL0 (subtilisin-like protease SBT3.7 OS=Cucurbita moschata OX=3662 GN=LOC111437655 PE=3 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 4.8e-279
Identity = 515/785 (65.61%), Postives = 609/785 (77.58%), Query Frame = 0

Query: 1   MVNGQAP---LIVGVITIYALFSMFAYKPMAEADDQNRKVYIVYLGERQYEDSKLTTESH 60
           M N QAP   L+V V+TIY +FS      M EADDQN KV+IVYLGER Y+D  LTT+SH
Sbjct: 1   MENNQAPSFMLVVAVVTIYVVFS-----AMGEADDQNSKVHIVYLGERPYDDVTLTTDSH 60

Query: 61  HDLLATVLGSKEKSSESMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQT 120
           H+LL +VLGSKEKS ES+VYSYRHGFSG AAKLT SQAQKLA MP V RV P+  YKM+T
Sbjct: 61  HELLESVLGSKEKSLESIVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSPLYKMRT 120

Query: 121 TRSWDFLGLSSSPS-NSNLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGR 180
           TRSWDFLGLSSSPS +SNLL R KMGD +IIGVID+G WPESE+F D G+GPIPSRWKG 
Sbjct: 121 TRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGI 180

Query: 181 CESGAEFNSTNCNRKIIGARWYSKGFIDNYGAEALANEYLSPRDANGHGTHVASTAAGSF 240
           C+SG +FNS++CN+K+IGARW++   + ++G EA+  +YLS RD  GHGTH ASTA G+F
Sbjct: 181 CQSGEDFNSSHCNKKVIGARWFASALVADHGEEAVFMDYLSARDNEGHGTHTASTAGGAF 240

Query: 241 VTNIGYHGLGIGTVRGGAPLARLAIYKVLWTNSGQGSIADILLAIDEAIHDGVDVLSMSI 300
           V N+ Y G G GT+RGGAPLARLAIYKVLW++S  GS ADIL  IDEAIHDGVDVLSMSI
Sbjct: 241 VRNVSYFGNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSI 300

Query: 301 VGSIPSLPEFDELHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTM 360
             SIP   + +EL+  VA+G FHAIA+GISVVCAGGN+G +QQT+EN APW+ TVAA+T+
Sbjct: 301 GKSIPLFSDVNELN-PVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTI 360

Query: 361 DRAFLASI-ALGDNTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDGI 420
           DRAFLASI  LGDN TYLGQ+    KKD+VG L+                  +  RC G+
Sbjct: 361 DRAFLASITTLGDNATYLGQTFL--KKDIVGMLLA-----------------MDRRCAGL 420

Query: 421 FGNETFLSGKVV-LCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDNLLPFAIP 480
            G+   +SG VV LCF+D +A K     V   VM    AK  GII AGQ ND L P   P
Sbjct: 421 LGSNIPISGNVVLLCFTD-LAKKAGASNV---VMPGKPAKVVGIIYAGQHNDILGPCDTP 480

Query: 481 CILVDSDVGTKLYFYALHNSNPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKPD 540
           CI VD+ VGT+L+ Y L++    +R+R  RTI GKPISS+IAYFSSRGPNS+SP ILKPD
Sbjct: 481 CIHVDTHVGTQLFTYYLNDEKAFIRVRATRTITGKPISSRIAYFSSRGPNSISPGILKPD 540

Query: 541 IAAPGSNIVAAISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSALI 600
           IAAPG+NI+AA+ P++    KGFA+ SGTSMAAPHISGIVAL+KSL PTWSPA IKSALI
Sbjct: 541 IAAPGANIIAAVPPNHEGGDKGFAVMSGTSMAAPHISGIVALIKSLRPTWSPAVIKSALI 600

Query: 601 TTASAKDPSGLPIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYI-YYLCAM 660
           TTA  +D SGLPIFAEGSPPKVADPFDYGGGVV+ NAA DPGLIYDL+ TDYI YYLC+M
Sbjct: 601 TTARVEDLSGLPIFAEGSPPKVADPFDYGGGVVDANAAIDPGLIYDLEVTDYIYYYLCSM 660

Query: 661 GYQDSEISSITGQKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRAV 720
           GY +++IS ++ QK  CP ++ S+LDLNLPTIT+P LTNSTTVTRTVTNVGNLT+VY+AV
Sbjct: 661 GYTEADISHLSQQKTVCPSKRTSILDLNLPTITVPTLTNSTTVTRTVTNVGNLTAVYKAV 720

Query: 721 IDPPPGTKVSVEPSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPLS 779
           I  PPG+KV V+P  L F+S VKKISFKV  S+ + RNYGYSFG+LTW+DGVHLV+SPLS
Sbjct: 721 IKAPPGSKVRVKPRVLVFNSNVKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLS 756

BLAST of Spg021078 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 774.6 bits (1999), Expect = 7.4e-224
Identity = 406/774 (52.45%), Postives = 542/774 (70.03%), Query Frame = 0

Query: 14  TIYALFSMFAYKPMAEADDQNRKVYIVYLGERQYEDSKLTTESHHDLLATVLGSKEKSSE 73
           ++  + S+     +A A  ++ KV+IVYLGE+Q++D K  TESHH +L+++LGSK+ + E
Sbjct: 7   SVLVVLSLIIVLNVARASAKS-KVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHE 66

Query: 74  SMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQTTRSWDFLGLSSSPSNS 133
           SMVYSYRHGFSG AAKLTKSQA+K+A+ P V+ V+P+S+Y++ TTR WD+LG  S+ ++ 
Sbjct: 67  SMVYSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYELATTRIWDYLG-PSADNSK 126

Query: 134 NLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGRCESGAEFNSTNCNRKII 193
           NL+    MGD  IIGVID+G+WPESE+F D G+GP+PS WKG CE G  F STNCNRK+I
Sbjct: 127 NLVSDTNMGDQTIIGVIDTGVWPESESFNDYGVGPVPSHWKGGCEPGENFISTNCNRKLI 186

Query: 194 GARWYSKGFI-DNYGAEALANEYLSPRDANGHGTHVASTAAGSFVTNIGYHGLGIGTVRG 253
           GA+++  GF+ +N      + +Y+S RD +GHGTHVAS A GSFV N+ Y GLG GT+RG
Sbjct: 187 GAKYFINGFLAENQFNATESPDYISARDFDGHGTHVASIAGGSFVPNVSYKGLGRGTLRG 246

Query: 254 GAPLARLAIYKVLW-TNSGQG---SIADILLAIDEAIHDGVDVLSMSIVGSIPSLPEFDE 313
           GAP AR+A+YK  W  N   G   S +DI+ AIDEAIHDGVDVLS+S+ G +P   E D 
Sbjct: 247 GAPRARIAMYKACWYINELDGVTCSFSDIMKAIDEAIHDGVDVLSISLGGRVPLNSETD- 306

Query: 314 LHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTMDRAFLASIALGD 373
           L  G+A G FHA+A+GI VVCAGGN GP  QT+ NTAPWILTVAA+T+DR+F   I LG+
Sbjct: 307 LRDGIATGAFHAVAKGIVVVCAGGNAGPSSQTVVNTAPWILTVAATTLDRSFATPIILGN 366

Query: 374 NTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDGI-FGNETFLSGKVV 433
           N   LGQ+++   +     LV P    +  ID         G C+ +   +   ++GKVV
Sbjct: 367 NQVILGQAMYIGPELGFTSLVYPEDPGN-SIDT------FSGVCESLNLNSNRTMAGKVV 426

Query: 434 LCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDNLLPFA--IPCILVDSDVGTK 493
           LCF+    T   F  V+ A   V  A G G+I+A     NL P +   PC+ +D+++GT 
Sbjct: 427 LCFT----TARDFTVVSTAASIVKAAGGLGLIIARNPGYNLAPCSDDFPCVAIDNELGTD 486

Query: 494 LYFYALHNSNPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKPDIAAPGSNIVAA 553
           + FY  +  +PVV+++ +RT++G+P+ +K+A FSSRGPNS+SPAILKPDIAAPG +I+AA
Sbjct: 487 ILFYIRYTGSPVVKIQPSRTLVGEPVGTKVATFSSRGPNSISPAILKPDIAAPGVSILAA 546

Query: 554 ISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSALITTASAKDPSGL 613
            SP+++ +  GF M+SGTSMAAP ISG++ALLKSLHP WSPAA +SA++TTA   DP G 
Sbjct: 547 TSPNDTLNAGGFVMRSGTSMAAPVISGVIALLKSLHPDWSPAAFRSAIVTTAWRTDPFGE 606

Query: 614 PIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYIYYLCAMGYQDSEISSITG 673
            I AE S  KV DPFDYGGG+VNP  AA+PGLI D+D+ DY+ YLC+ GY DS IS + G
Sbjct: 607 QIAAESSSLKVPDPFDYGGGLVNPEKAAEPGLILDMDSQDYVLYLCSAGYNDSSISRLVG 666

Query: 674 QKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRAVIDPPPGTKVSVE 733
           +   C   KPSVLD+NLP+ITIP L +  T+TRTVTNVG + SVY+ +++PP G +V V 
Sbjct: 667 KVTVCSNPKPSVLDINLPSITIPNLKDEVTLTRTVTNVGPVDSVYKVLVEPPLGIQVVVT 726

Query: 734 PSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPLSVRINFL 780
           P  L F+S+ K +SF V +ST    N G+ FG+LTW+D +H V  P+SVR   L
Sbjct: 727 PETLVFNSKTKSVSFTVIVSTTHKINTGFYFGSLTWTDSIHNVVIPVSVRTQIL 766

BLAST of Spg021078 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 768.5 bits (1983), Expect = 5.3e-222
Identity = 402/755 (53.25%), Postives = 531/755 (70.33%), Query Frame = 0

Query: 36  KVYIVYLGERQYEDSKLTTESHHDLLATVLGSKEKSSESMVYSYRHGFSGLAAKLTKSQA 95
           KV+IVYLGE+Q++D +  TESHH +L ++LGSKE +  SMV+SYRHGFSG AAKLTKSQA
Sbjct: 29  KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQA 88

Query: 96  QKLAEMPGVVRVLPNSFYKMQTTRSWDFLGLSSSPSNSNLLRRGKMGDGIIIGVIDSGIW 155
           +KLA++P VV V P+SFY++ TTR+WD+LGLS + +  NLL    MG+ +IIG++DSG+W
Sbjct: 89  KKLADLPEVVHVTPDSFYQLDTTRTWDYLGLSVA-NPKNLLNDTNMGEEVIIGIVDSGVW 148

Query: 156 PESEAFRDNGLGPIPSRWKGRCESGAEFNSTNCNRKIIGARWYSKGFIDNY----GAEAL 215
           PESE F DNG+GP+PS WKG C SG  F S+ CN+K+IGA+++  GF+  +      E+L
Sbjct: 149 PESEVFNDNGIGPVPSHWKGGCVSGENFTSSQCNKKLIGAKYFINGFLATHESFNSTESL 208

Query: 216 ANEYLSPRDANGHGTHVASTAAGSFVTNIGYHGLGIGTVRGGAPLARLAIYKVLW----T 275
             +++SPRD +GHGTHVA+ A GS+V +I Y GL  GTVRGGAP AR+A+YK  W     
Sbjct: 209 --DFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRARIAMYKACWYLDRF 268

Query: 276 NSGQGSIADILLAIDEAIHDGVDVLSMSIVGSIPSLPEFDELHGGVAIGLFHAIARGISV 335
           +    S ADIL A+DEA+HDGVDVLS+SI    P  PE D +   +A G FHA+ +GI+V
Sbjct: 269 DINTCSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETD-VRAVIATGAFHAVLKGITV 328

Query: 336 VCAGGNDGPLQQTMENTAPWILTVAASTMDRAFLASIALGDNTTYLGQSLFSAKKDVVGQ 395
           VC+GGN GP  QT+ NTAPWILTVAA+T+DR+F   I LG+N   LGQ++++  +     
Sbjct: 329 VCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNKLILGQAMYTGPELGFTS 388

Query: 396 LVLPPTTRSLHIDVLYWIYYIGGRCDGIFGNETF-LSGKVVLCFSDGVATKTIFETVAVA 455
           LV P    + +           G C+ +F N    ++GKVVLCF+    T T + TV+ A
Sbjct: 389 LVYPENPGNSNES-------FSGDCELLFFNSNHTMAGKVVLCFT----TSTRYITVSSA 448

Query: 456 VMAVAKAKGRGIIVAGQQNDNLLPFA--IPCILVDSDVGTKLYFYALHNSNPVVRLRQAR 515
           V  V +A G G+IVA    DNL P     PC+ VD ++GT +  Y      PVV+++ ++
Sbjct: 449 VSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTDILLYIRSTGLPVVKIQPSK 508

Query: 516 TIIGKPISSKIAYFSSRGPNSVSPAILKPDIAAPGSNIVAAISPHNSFSYKGFAMQSGTS 575
           T++G+P+ +K+A FSSRGPNS+ PAILKPDIAAPG +I+AA + + +F+ +GF   SGTS
Sbjct: 509 TLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAATTTNKTFNDRGFIFLSGTS 568

Query: 576 MAAPHISGIVALLKSLHPTWSPAAIKSALITTASAKDPSGLPIFAEGSPPKVADPFDYGG 635
           MAAP ISG+VALLK+LH  WSPAAI+SA++TTA   DP G  IFAEGSP K+ADPFDYGG
Sbjct: 569 MAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKLADPFDYGG 628

Query: 636 GVVNPNAAADPGLIYDLDTTDYIYYLCAMGYQDSEISSITGQKRKCPLQKPSVLDLNLPT 695
           G+VNP  AA PGL+YDL   DY+ Y+C++GY ++ IS + G+   C   KPSVLD NLP+
Sbjct: 629 GLVNPEKAAKPGLVYDLGLEDYVLYMCSVGYNETSISQLVGKGTVCSNPKPSVLDFNLPS 688

Query: 696 ITIPALTNSTTVTRTVTNVGNLTSVYRAVIDPPPGTKVSVEPSKLAFSSQVKKISFKVTI 755
           ITIP L +  T+TRT+TNVG L SVY+ VI+PP G +V+V P  L F+S  K++SFKV +
Sbjct: 689 ITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTTKRVSFKVKV 748

Query: 756 STAVYRNYGYSFGTLTWSDGVHLVQSPLSVRINFL 780
           ST    N GY FG+LTWSD +H V  PLSVR   L
Sbjct: 749 STTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQIL 768

BLAST of Spg021078 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 763.1 bits (1969), Expect = 2.2e-220
Identity = 396/779 (50.83%), Postives = 537/779 (68.93%), Query Frame = 0

Query: 14  TIYALFSMFAY----KPMAEADDQNRKVYIVYLGERQYEDSKLTTESHHDLLATVLGSKE 73
           +IY + S+  +    +         RKV+IVYLGE+Q++D +  TESHH +L ++LGSKE
Sbjct: 7   SIYVVLSLVIFLNVQRSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKE 66

Query: 74  KSSESMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQTTRSWDFLGLSSS 133
            +++SMVYSYRHGFSG AAKLT+SQA+K+A++P VV V+P+SFYK+ TTR+WD+LGLS++
Sbjct: 67  DANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAA 126

Query: 134 PSNSNLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGRCESGAEFNSTNCN 193
              S LL    MG+ IIIGVID+G+WPESE F D+G GP+PS WKG CE+G  FNS+NCN
Sbjct: 127 NPKS-LLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCN 186

Query: 194 RKIIGARWYSKGFIDNYGAEALAN--EYLSPRDANGHGTHVASTAAGSFVTNIGYHGLGI 253
           +K+IGA+++  GF+    +    N  +++SPRD +GHGTHV++ A GSFV NI Y GL  
Sbjct: 187 KKLIGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAG 246

Query: 254 GTVRGGAPLARLAIYKVLW----TNSGQGSIADILLAIDEAIHDGVDVLSMSIVGSIPSL 313
           GTVRGGAP A +A+YK  W     ++   S ADIL A+DEA+HDGVDVLS+S+  S+P  
Sbjct: 247 GTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLY 306

Query: 314 PEFDELHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTMDRAFLAS 373
            E D +  G+  G FHA+ +GI+VVC+GGN GP   T+ NTAPWI+TVAA+T+DR+F   
Sbjct: 307 GETD-IRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATP 366

Query: 374 IALGDNTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDG-IFGNETFL 433
           + LG+N   LGQ++++        LV P    + +           G C+  +F +   +
Sbjct: 367 LTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNES-------FSGTCEELLFNSNRTM 426

Query: 434 SGKVVLCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDNLLPFA--IPCILVDS 493
            GKVVLCF+    T      V  A   V +A G G+I+A      + P     PC+ VD 
Sbjct: 427 EGKVVLCFT----TSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDW 486

Query: 494 DVGTKLYFYALHNSNPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKPDIAAPGS 553
           ++GT +  Y   + +PVV+++ ++T++G+P+ +K+A FSSRGPNS++PAILKPDIAAPG 
Sbjct: 487 ELGTDILLYTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGV 546

Query: 554 NIVAAISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSALITTASAK 613
           +I+AA + + +FS +GF M SGTSMAAP ISG+ ALLK+LH  WSPAAI+SA++TTA   
Sbjct: 547 SILAA-TTNTTFSDQGFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKT 606

Query: 614 DPSGLPIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYIYYLCAMGYQDSEI 673
           DP G  IFAEGSPPK+ADPFDYGGG+VNP  +A+PGL+YD+   DY+ Y+C++GY ++ I
Sbjct: 607 DPFGEQIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSI 666

Query: 674 SSITGQKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRAVIDPPPGT 733
           S + G+   C   KPSVLD NLP+ITIP L +  T+TRTVTNVG L SVYR  ++PP G 
Sbjct: 667 SQLIGKTTVCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGF 726

Query: 734 KVSVEPSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPLSVRINFL 780
           +V+V P  L F+S  KK+ FKV +ST    N GY FG+LTWSD +H V  PLSVR   L
Sbjct: 727 QVTVTPETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQIL 771

BLAST of Spg021078 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 761.9 bits (1966), Expect = 4.9e-220
Identity = 393/754 (52.12%), Postives = 529/754 (70.16%), Query Frame = 0

Query: 35  RKVYIVYLGERQYEDSKLTTESHHDLLATVLGSKEKSSESMVYSYRHGFSGLAAKLTKSQ 94
           RKV+IVYLGE+Q++D +  TESHH +L ++LGSKE +++SMVYSYRHGFSG AAKLT+SQ
Sbjct: 48  RKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLTESQ 107

Query: 95  AQKLAEMPGVVRVLPNSFYKMQTTRSWDFLGLSSSPSNSNLLRRGKMGDGIIIGVIDSGI 154
           A+K+A++P VV V+P+SFYK+ TTR+WD+LGLS++   S LL    MG+ IIIGVID+G+
Sbjct: 108 AKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKS-LLHETNMGEQIIIGVIDTGV 167

Query: 155 WPESEAFRDNGLGPIPSRWKGRCESGAEFNSTNCNRKIIGARWYSKGFIDNYGAEALAN- 214
           WPESE F D+G GP+PS WKG CE+G  FNS+NCN+K+IGA+++  GF+    +    N 
Sbjct: 168 WPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGAKYFINGFLAENESFNSTNS 227

Query: 215 -EYLSPRDANGHGTHVASTAAGSFVTNIGYHGLGIGTVRGGAPLARLAIYKVLW----TN 274
            +++SPRD +GHGTHV++ A GSFV NI Y GL  GTVRGGAP A +A+YK  W     +
Sbjct: 228 LDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAMYKACWYLDDDD 287

Query: 275 SGQGSIADILLAIDEAIHDGVDVLSMSIVGSIPSLPEFDELHGGVAIGLFHAIARGISVV 334
           +   S ADIL A+DEA+HDGVDVLS+S+  S+P   E D +  G+  G FHA+ +GI+VV
Sbjct: 288 TTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETD-IRDGITTGAFHAVLKGITVV 347

Query: 335 CAGGNDGPLQQTMENTAPWILTVAASTMDRAFLASIALGDNTTYLGQSLFSAKKDVVGQL 394
           C+GGN GP   T+ NTAPWI+TVAA+T+DR+F   + LG+N   LGQ++++        L
Sbjct: 348 CSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLTLGNNKVILGQAMYTGPGLGFTSL 407

Query: 395 VLPPTTRSLHIDVLYWIYYIGGRCDG-IFGNETFLSGKVVLCFSDGVATKTIFETVAVAV 454
           V P    + +           G C+  +F +   + GKVVLCF+    T      V  A 
Sbjct: 408 VYPENPGNSNES-------FSGTCEELLFNSNRTMEGKVVLCFT----TSPYGGAVLSAA 467

Query: 455 MAVAKAKGRGIIVAGQQNDNLLPFA--IPCILVDSDVGTKLYFYALHNSNPVVRLRQART 514
             V +A G G+I+A      + P     PC+ VD ++GT +  Y   + +PVV+++ ++T
Sbjct: 468 RYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKT 527

Query: 515 IIGKPISSKIAYFSSRGPNSVSPAILKPDIAAPGSNIVAAISPHNSFSYKGFAMQSGTSM 574
           ++G+P+ +K+A FSSRGPNS++PAILKPDIAAPG +I+AA + + +FS +GF M SGTSM
Sbjct: 528 LVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAA-TTNTTFSDQGFIMLSGTSM 587

Query: 575 AAPHISGIVALLKSLHPTWSPAAIKSALITTASAKDPSGLPIFAEGSPPKVADPFDYGGG 634
           AAP ISG+ ALLK+LH  WSPAAI+SA++TTA   DP G  IFAEGSPPK+ADPFDYGGG
Sbjct: 588 AAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPPKLADPFDYGGG 647

Query: 635 VVNPNAAADPGLIYDLDTTDYIYYLCAMGYQDSEISSITGQKRKCPLQKPSVLDLNLPTI 694
           +VNP  +A+PGL+YD+   DY+ Y+C++GY ++ IS + G+   C   KPSVLD NLP+I
Sbjct: 648 LVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIGKTTVCSNPKPSVLDFNLPSI 707

Query: 695 TIPALTNSTTVTRTVTNVGNLTSVYRAVIDPPPGTKVSVEPSKLAFSSQVKKISFKVTIS 754
           TIP L +  T+TRTVTNVG L SVYR  ++PP G +V+V P  L F+S  KK+ FKV +S
Sbjct: 708 TIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTKKVYFKVKVS 767

Query: 755 TAVYRNYGYSFGTLTWSDGVHLVQSPLSVRINFL 780
           T    N GY FG+LTWSD +H V  PLSVR   L
Sbjct: 768 TTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQIL 787

BLAST of Spg021078 vs. TAIR 10
Match: AT1G32940.1 (Subtilase family protein )

HSP 1 Score: 761.5 bits (1965), Expect = 6.5e-220
Identity = 404/776 (52.06%), Postives = 532/776 (68.56%), Query Frame = 0

Query: 13  ITIYALFSMFAYKPMAEADDQNRKVYIVYLGERQYEDSKLTTESHHDLLATVLGSKEKSS 72
           + +  + S+     +  A D++ KV+IVYLGE+Q++D +  +ESHH +L+++LGSK  + 
Sbjct: 6   VLLVLVLSLVIVLNVVRASDES-KVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAH 65

Query: 73  ESMVYSYRHGFSGLAAKLTKSQAQKLAEMPGVVRVLPNSFYKMQTTRSWDFLGLSSSPSN 132
           ESMVYSYRHGFSG AAKLT+SQA+KLA+ P VV V+ +SFY++ TTR+WD+LGLS +  N
Sbjct: 66  ESMVYSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGLSVANPN 125

Query: 133 SNLLRRGKMGDGIIIGVIDSGIWPESEAFRDNGLGPIPSRWKGRCESGAEFNSTNCNRKI 192
            NLL    MGD +IIG ID+G+WPESE+F DNG+GPIPS WKG CESG +F STNCNRK+
Sbjct: 126 -NLLNDTNMGDQVIIGFIDTGVWPESESFNDNGVGPIPSHWKGGCESGEKFISTNCNRKL 185

Query: 193 IGARWYSKGFI-DNYGAEAL-ANEYLSPRDANGHGTHVASTAAGSFVTNIGYHGLGIGTV 252
           IGA+++  GF+ +N G     + +Y+S RD  GHGTH AS A GSFV NI Y GL  G +
Sbjct: 186 IGAKYFINGFLAENEGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYKGLAGGNL 245

Query: 253 RGGAPLARLAIYKVLWTNSGQGSIA----DILLAIDEAIHDGVDVLSMSIVGSIPSLPEF 312
           RGGAP AR+AIYK  W     G++A    DIL A+DE++HDGVDVLS+S+   IP  PE 
Sbjct: 246 RGGAPRARIAIYKACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPET 305

Query: 313 DELHGGVAIGLFHAIARGISVVCAGGNDGPLQQTMENTAPWILTVAASTMDRAFLASIAL 372
           D L   +A G FHA+A+GI VVCAGGN GP  QT+ NTAPWI+TVAA+T+DR+F   I L
Sbjct: 306 D-LRDRIATGAFHAVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPITL 365

Query: 373 GDNTTYLGQSLFSAKKDVVGQLVLPPTTRSLHIDVLYWIYYIGGRCDGIFGN-ETFLSGK 432
           G+    LGQ+L++ ++     LV P           +      G C+ +  N    ++GK
Sbjct: 366 GNRKVILGQALYTGQELGFTSLVYPENAG-------FTNETFSGVCERLNLNPNRTMAGK 425

Query: 433 VVLCFSDGVATKTIFETVAVAVMAVAKAKGRGIIVAGQQNDNLLPFA--IPCILVDSDVG 492
           VVLCF+    T T+F  V+ A   V  A G G+I+A     NL P     PC+ +D ++G
Sbjct: 426 VVLCFT----TNTLFTAVSRAASYVKAAGGLGVIIARNPGYNLTPCRDDFPCVAIDYELG 485

Query: 493 TKLYFYALHNSNPVVRLRQARTIIGKPISSKIAYFSSRGPNSVSPAILKPDIAAPGSNIV 552
           T +  Y     +PVV+++ +RT++G+P+ +K+A FSSRGPNS+SPAILKPDI APG +I+
Sbjct: 486 TDVLLYIRSTRSPVVKIQPSRTLVGQPVGTKVATFSSRGPNSISPAILKPDIGAPGVSIL 545

Query: 553 AAISPHNSFSYKGFAMQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSALITTASAKDPS 612
           AA SP ++ S  GF + +GTSMAAP ++G+VALLK+LHP WSPAA +SA++TTA   DP 
Sbjct: 546 AATSPDSNSSVGGFDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRSAIVTTAWRTDPF 605

Query: 613 GLPIFAEGSPPKVADPFDYGGGVVNPNAAADPGLIYDLDTTDYIYYLCAMGYQDSEISSI 672
           G  IFAEGS  KVADPFDYGGG+VNP  AADPGLIYD+   DYI YLC+ GY DS I+ +
Sbjct: 606 GEQIFAEGSSRKVADPFDYGGGIVNPEKAADPGLIYDMGPRDYILYLCSAGYNDSSITQL 665

Query: 673 TGQKRKCPLQKPSVLDLNLPTITIPALTNSTTVTRTVTNVGNLTSVYRAVIDPPPGTKVS 732
            G    C   K SVLD+NLP+ITIP L +  T+TRTVTNVG + SVY+ V++PP G +V 
Sbjct: 666 VGNVTVCSTPKTSVLDVNLPSITIPDLKDEVTLTRTVTNVGTVDSVYKVVVEPPLGIQVV 725

Query: 733 VEPSKLAFSSQVKKISFKVTISTAVYRNYGYSFGTLTWSDGVHLVQSPLSVRINFL 780
           V P  L F+S+ K +SF V +ST    N G+ FG L W+D +H V  P+SVR   L
Sbjct: 726 VAPETLVFNSKTKNVSFTVRVSTTHKINTGFYFGNLIWTDSMHNVTIPVSVRTQIL 767