Spg021017 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg021017
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionThreonylcarbamoyladenosine tRNA methylthiotransferase
Locationscaffold9: 1392984 .. 1394922 (-)
RNA-Seq ExpressionSpg021017
SyntenySpg021017
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGAAGAAAATGTCAATGGCTGTGGATCTATGGTTGAGGAGGTGTTCTTGATTTCTCCCTTTCATGATAAGGGCATAAGTTTTGTAGCAGGCTTGACAATAGGATTTTCAGAGGGGTTAGAGATCTAGGGAGGAAGTTTGTGCTAGGTGTAATGCTTCTTTGTGGACGTCCTCTATCGAACTTTTTATAATTACCAGCTTGGTTTTATTCTTTTAGATTAGAATCCCTTCTTGTAGTTAGTTGTTGGTTTCTGTTTGTGGTTTTGTTTTTTGTATGCTCTTGTACATTTTGTCATGTTTCTTAAAAAAAAAAAAAAAAAGAAAAAAAAGAAAAATTGATTTAAGTGTCAGAAAGGGGAAAATTGAATTTTTGTCGGAGGGAGGGTTGTGGCAGTTTCGGAGTTTTTTAGCTCTTCCTTTCGGATGTATATAGCTGAAAACGTATAAAAGAACTCTGAAAACGTTTGCCAATAGATCAAGTTTTGGGGCTGAACGCATCTTATTGCTTCTCTTTGGTCTTATGTATGTGGTCTTCCAAAATGATTTTCTTTCTATTTAAATTTTTTTTTTTCCTTTTCAGTTAAATAATGGTTGTGGGTGGTTTATAAAGAGTATGCAGTTATTATGTTTCTTCTTTATTCCCCTTGGTTTGCTGCTGTTGCTTGAAATTTCACGTTTCTTAAAAGAAAATCAAGTTTTTTGGTGGTCTGAAGCATTCGTCTTTACTAAGCCAGGATTTATATACTTGCATCAAGGAATTACAGACACCTTTGTGAACTTATGAAATGATTTTTGCGATAAACTCCCTCTCTATCTCTTCTTCTTTTCTCTGTTTCTTAGTGGAAGCTGTATTTGTTCTTCCTCATGTGAAAATGGAACCATTGTTATTAAACTGTTCCTTCATCAATTTTAATTCGCACATGCATTTTAGTCGCAACATGGAAGGGAACTACATTCTGTGATCGATATCTCATAAAACTGCTCTTTCTGTGTACCCTTTGATCATCTAATGTTTCAAAGAACAGCTGTTTCTTTTTTCATTAGCTCAATTTATTTTGCTTCTCTAGGTTGGCCATACCAAGGGATACATACAGGTGCTAGTAATTGCCCCGGAAACTATGCTAGGAACTTCTGCTATGGTGAAGATAACATCAGTTGGAAGGTGGTCCGTTTTTGGGGAAGTGATTGAAACCATCAGCACCAAACATCATAAAACAACTACTCTCGAAGACATGCTGATTCGAGACGAAGTTCCTCCCTGCTCTAACGCTCACGAAATGTGTGCATGTTCCATGGAACCCAAACTTTGTGCTTGTGGACCAGAAAGCTGCAAAGGACCAGTTGCTCTGGATGATAAAGATGATTCCTCAAGGAATATCCCATTGCCCGAAGAGTCGAAACGGAAGAATCTGATTGGATGGCTATTGAGGAGACGGAAGGGCCATGTGCCCAAAAGGGAGGAGAGAGAAAGTCCCATAGTATCTGAGAGAAAGCAGGCATTGGCTGGAGATTGGGGCGTCATCGATAGGATTCTTGTGGGAGGAATACTAATCAGTATCTTTACAATTGTTGGTCTACTGTTTCATCTTGGATCCACCACACTCTCCTCCAGTTGGTGATATTATACAATGCTAATTTGCATTGAACCATACTTAAGAAAGTCTGTAATGAGAGTTTTATTGTTCCTTTTTCAGCTTTCATCTATAGAGGTAGTTTTTTTTACTGTCACAATTTCAAGTGTAATTTGAGATCTCAGTGATTTTGTTTTCAATTTTGCAGTTCTCTCTTTAGATTTTCATCTACTTCCATGACTTAGAATCTACTAGATTACATAGGAACAATCTCAAATTTGGTTTTCTCTACCAATTAAGAATGTATTAGGGAATAGAATAGAAGTGCTTTTGTTACAAGTATTTTAATAAAAAGTAATTAATTA

mRNA sequence

ATGATTGAAGAAAATGTCAATGGCTGTGGATCTATGGTTGAGGAGGTTGGCCATACCAAGGGATACATACAGGTGCTAGTAATTGCCCCGGAAACTATGCTAGGAACTTCTGCTATGGTGAAGATAACATCAGTTGGAAGGTGGTCCGTTTTTGGGGAAGTGATTGAAACCATCAGCACCAAACATCATAAAACAACTACTCTCGAAGACATGCTGATTCGAGACGAAGTTCCTCCCTGCTCTAACGCTCACGAAATGTGTGCATGTTCCATGGAACCCAAACTTTGTGCTTGTGGACCAGAAAGCTGCAAAGGACCAGTTGCTCTGGATGATAAAGATGATTCCTCAAGGAATATCCCATTGCCCGAAGAGTCGAAACGGAAGAATCTGATTGGATGGCTATTGAGGAGACGGAAGGGCCATGTGCCCAAAAGGGAGGAGAGAGAAAGTCCCATAGTATCTGAGAGAAAGCAGGCATTGGCTGGAGATTGGGGCGTCATCGATAGGATTCTTGTGGGAGGAATACTAATCAGTATCTTTACAATTGTTGGTCTACTGTTTCATCTTGGATCCACCACACTCTCCTCCAGTTGGTGA

Coding sequence (CDS)

ATGATTGAAGAAAATGTCAATGGCTGTGGATCTATGGTTGAGGAGGTTGGCCATACCAAGGGATACATACAGGTGCTAGTAATTGCCCCGGAAACTATGCTAGGAACTTCTGCTATGGTGAAGATAACATCAGTTGGAAGGTGGTCCGTTTTTGGGGAAGTGATTGAAACCATCAGCACCAAACATCATAAAACAACTACTCTCGAAGACATGCTGATTCGAGACGAAGTTCCTCCCTGCTCTAACGCTCACGAAATGTGTGCATGTTCCATGGAACCCAAACTTTGTGCTTGTGGACCAGAAAGCTGCAAAGGACCAGTTGCTCTGGATGATAAAGATGATTCCTCAAGGAATATCCCATTGCCCGAAGAGTCGAAACGGAAGAATCTGATTGGATGGCTATTGAGGAGACGGAAGGGCCATGTGCCCAAAAGGGAGGAGAGAGAAAGTCCCATAGTATCTGAGAGAAAGCAGGCATTGGCTGGAGATTGGGGCGTCATCGATAGGATTCTTGTGGGAGGAATACTAATCAGTATCTTTACAATTGTTGGTCTACTGTTTCATCTTGGATCCACCACACTCTCCTCCAGTTGGTGA

Protein sequence

MIEENVNGCGSMVEEVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDSSRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAGDWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSSW
Homology
[Close]
BLAST of Spg021017 vs. NCBI nr
Match: XP_038890156.1 (threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 324.3 bits (830), Expect = 7.2e-85
Identity = 164/187 (87.70%), Postives = 169/187 (90.37%), Query Frame = 0 

Query: 16  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRD 75
           VGHTKGYIQVLVIAPETMLGTSAMVKITS GRWSVFGEVIETISTKHHKTTTLED  IRD
Sbjct: 447 VGHTKGYIQVLVIAPETMLGTSAMVKITSFGRWSVFGEVIETISTKHHKTTTLEDAPIRD 506

Query: 76  EVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDSSRNIPLPEESKRKNLIGWLL 135
           +VPPCSNAHE CACS+EP+ CACGPESCKG VA DDKDDSSRNIPLPEE KRKNL+ W L
Sbjct: 507 KVPPCSNAHETCACSIEPESCACGPESCKGAVATDDKDDSSRNIPLPEEPKRKNLVEWAL 566

Query: 136 RRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGS 195
           RRRK HVPKREERESPIVSERKQALAG    +WGVIDRILVGGILIS  TI GLLFHLGS
Sbjct: 567 RRRKSHVPKREERESPIVSERKQALAGGWLDEWGVIDRILVGGILISTLTIFGLLFHLGS 626

Query: 196 TTLSSSW 199
           TTLSSSW
Sbjct: 627 TTLSSSW 633

[Close]
BLAST of Spg021017 vs. NCBI nr
Match: XP_038890157.1 (threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2 [Benincasa hispida])

HSP 1 Score: 324.3 bits (830), Expect = 7.2e-85
Identity = 164/187 (87.70%), Postives = 169/187 (90.37%), Query Frame = 0 

Query: 16  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRD 75
           VGHTKGYIQVLVIAPETMLGTSAMVKITS GRWSVFGEVIETISTKHHKTTTLED  IRD
Sbjct: 377 VGHTKGYIQVLVIAPETMLGTSAMVKITSFGRWSVFGEVIETISTKHHKTTTLEDAPIRD 436

Query: 76  EVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDSSRNIPLPEESKRKNLIGWLL 135
           +VPPCSNAHE CACS+EP+ CACGPESCKG VA DDKDDSSRNIPLPEE KRKNL+ W L
Sbjct: 437 KVPPCSNAHETCACSIEPESCACGPESCKGAVATDDKDDSSRNIPLPEEPKRKNLVEWAL 496

Query: 136 RRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGS 195
           RRRK HVPKREERESPIVSERKQALAG    +WGVIDRILVGGILIS  TI GLLFHLGS
Sbjct: 497 RRRKSHVPKREERESPIVSERKQALAGGWLDEWGVIDRILVGGILISTLTIFGLLFHLGS 556

Query: 196 TTLSSSW 199
           TTLSSSW
Sbjct: 557 TTLSSSW 563

[Close]
BLAST of Spg021017 vs. NCBI nr
Match: XP_023524910.1 (threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 310.8 bits (795), Expect = 8.2e-81
Identity = 158/187 (84.49%), Postives = 169/187 (90.37%), Query Frame = 0 

Query: 16  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRD 75
           VGHTKGYIQVLVI PE MLGTSA+VKITSVGRWSVFGEVIETI TKHHKTT LEDM I+ 
Sbjct: 447 VGHTKGYIQVLVIGPEAMLGTSALVKITSVGRWSVFGEVIETIVTKHHKTTALEDMPIQA 506

Query: 76  EVPPCSNAHEMCACSMEPKLCACGPES-CKGPVALDDKDDSSRNIPLPEESKRKNLIGWL 135
           +VPPCSNAHE CACS+EP+ CACG ES CKGPVALD+KDDSSRNIPLPEE KRKNL+ WL
Sbjct: 507 KVPPCSNAHETCACSVEPEACACGAESCCKGPVALDEKDDSSRNIPLPEEPKRKNLVEWL 566

Query: 136 LRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLG 195
           LR+RK HVPKREERESP+VSERKQA AG    +WGV+DRILVGGILISIFTIVGLLFHLG
Sbjct: 567 LRKRKSHVPKREERESPVVSERKQASAGGSMSEWGVVDRILVGGILISIFTIVGLLFHLG 626

Query: 196 STTLSSS 198
           STTLSSS
Sbjct: 627 STTLSSS 633

[Close]
BLAST of Spg021017 vs. NCBI nr
Match: XP_022949069.1 (threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita moschata])

HSP 1 Score: 308.9 bits (790), Expect = 3.1e-80
Identity = 157/187 (83.96%), Postives = 169/187 (90.37%), Query Frame = 0 

Query: 16  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRD 75
           VGHTKGYIQVLVI  ETMLGTSA+VKITSVGRWSVFGEVIETI TKHHKTT LEDM I+ 
Sbjct: 449 VGHTKGYIQVLVIGHETMLGTSALVKITSVGRWSVFGEVIETIVTKHHKTTALEDMPIQA 508

Query: 76  EVPPCSNAHEMCACSMEPKLCACGPES-CKGPVALDDKDDSSRNIPLPEESKRKNLIGWL 135
           +VPPCS+AHE CACS+EP+ CACGPES CKGPVALD+KDDSSRNIPLPEE KRKNL+ WL
Sbjct: 509 KVPPCSDAHETCACSVEPEACACGPESCCKGPVALDEKDDSSRNIPLPEEPKRKNLVEWL 568

Query: 136 LRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLG 195
           LR+RK H PKREERESP+VSERKQA AG    +WGV+DRILVGGILISIFTIVGLLFHLG
Sbjct: 569 LRKRKSHAPKREERESPVVSERKQASAGGSMDEWGVVDRILVGGILISIFTIVGLLFHLG 628

Query: 196 STTLSSS 198
           STTLSSS
Sbjct: 629 STTLSSS 635

[Close]
BLAST of Spg021017 vs. NCBI nr
Match: XP_022997808.1 (threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita maxima])

HSP 1 Score: 303.9 bits (777), Expect = 1.0e-78
Identity = 155/187 (82.89%), Postives = 167/187 (89.30%), Query Frame = 0 

Query: 16  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRD 75
           VGHTKGYIQVLVI  E MLGTSA+VKITSVGRWSVFGEVIETI TKHHKTT LEDM I+ 
Sbjct: 450 VGHTKGYIQVLVIGHEAMLGTSALVKITSVGRWSVFGEVIETIVTKHHKTTALEDMPIQA 509

Query: 76  EVPPCSNAHEMCACSMEPKLCACGPES-CKGPVALDDKDDSSRNIPLPEESKRKNLIGWL 135
           +VPPCSN+HE CACS+EP+ CACGPES CKGPVALD+KDDSSRNIPLPEE KRKNL+ WL
Sbjct: 510 KVPPCSNSHETCACSVEPEACACGPESCCKGPVALDEKDDSSRNIPLPEEPKRKNLVEWL 569

Query: 136 LRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLG 195
           LR+RK HVPKREE ESP+VS RKQA AG    +WGV+DRILVGGILISIFTIVGLLFHLG
Sbjct: 570 LRKRKSHVPKREEGESPVVSGRKQASAGGSMSEWGVVDRILVGGILISIFTIVGLLFHLG 629

Query: 196 STTLSSS 198
           STTLSSS
Sbjct: 630 STTLSSS 636

[Close]
BLAST of Spg021017 vs. ExPASy TrEMBL
Match: A0A6J1GBS1 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucurbita moschata OX=3662 GN=LOC111452531 PE=3 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 1.5e-80
Identity = 157/187 (83.96%), Postives = 169/187 (90.37%), Query Frame = 0 

Query: 16  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRD 75
           VGHTKGYIQVLVI  ETMLGTSA+VKITSVGRWSVFGEVIETI TKHHKTT LEDM I+ 
Sbjct: 449 VGHTKGYIQVLVIGHETMLGTSALVKITSVGRWSVFGEVIETIVTKHHKTTALEDMPIQA 508

Query: 76  EVPPCSNAHEMCACSMEPKLCACGPES-CKGPVALDDKDDSSRNIPLPEESKRKNLIGWL 135
           +VPPCS+AHE CACS+EP+ CACGPES CKGPVALD+KDDSSRNIPLPEE KRKNL+ WL
Sbjct: 509 KVPPCSDAHETCACSVEPEACACGPESCCKGPVALDEKDDSSRNIPLPEEPKRKNLVEWL 568

Query: 136 LRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLG 195
           LR+RK H PKREERESP+VSERKQA AG    +WGV+DRILVGGILISIFTIVGLLFHLG
Sbjct: 569 LRKRKSHAPKREERESPVVSERKQASAGGSMDEWGVVDRILVGGILISIFTIVGLLFHLG 628

Query: 196 STTLSSS 198
           STTLSSS
Sbjct: 629 STTLSSS 635

[Close]
BLAST of Spg021017 vs. ExPASy TrEMBL
Match: A0A6J1KEZ5 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucurbita maxima OX=3661 GN=LOC111492654 PE=3 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 4.9e-79
Identity = 155/187 (82.89%), Postives = 167/187 (89.30%), Query Frame = 0 

Query: 16  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRD 75
           VGHTKGYIQVLVI  E MLGTSA+VKITSVGRWSVFGEVIETI TKHHKTT LEDM I+ 
Sbjct: 450 VGHTKGYIQVLVIGHEAMLGTSALVKITSVGRWSVFGEVIETIVTKHHKTTALEDMPIQA 509

Query: 76  EVPPCSNAHEMCACSMEPKLCACGPES-CKGPVALDDKDDSSRNIPLPEESKRKNLIGWL 135
           +VPPCSN+HE CACS+EP+ CACGPES CKGPVALD+KDDSSRNIPLPEE KRKNL+ WL
Sbjct: 510 KVPPCSNSHETCACSVEPEACACGPESCCKGPVALDEKDDSSRNIPLPEEPKRKNLVEWL 569

Query: 136 LRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLG 195
           LR+RK HVPKREE ESP+VS RKQA AG    +WGV+DRILVGGILISIFTIVGLLFHLG
Sbjct: 570 LRKRKSHVPKREEGESPVVSGRKQASAGGSMSEWGVVDRILVGGILISIFTIVGLLFHLG 629

Query: 196 STTLSSS 198
           STTLSSS
Sbjct: 630 STTLSSS 636

[Close]
BLAST of Spg021017 vs. ExPASy TrEMBL
Match: A0A0A0KSZ4 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis sativus OX=3659 GN=Csa_5G652200 PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 1.2e-74
Identity = 147/188 (78.19%), Postives = 161/188 (85.64%), Query Frame = 0 

Query: 16  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRD 75
           VGHTKGYIQVLVIAPETMLGTSA VKITS+GRWSVFGEVIE ISTKHHKTTTLED L +D
Sbjct: 448 VGHTKGYIQVLVIAPETMLGTSATVKITSIGRWSVFGEVIEIISTKHHKTTTLEDTLTQD 507

Query: 76  EVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDSSRNIPLPEESKRKNLIGWLL 135
           +V PCSN HE CACS EP+ CACG ESCKG VA+ D+ +SSRN+P PEE KRKNLI W+L
Sbjct: 508 KVSPCSNTHETCACSTEPESCACGLESCKGAVAVGDEVNSSRNVPSPEEPKRKNLIEWVL 567

Query: 136 RRRKGHV-PKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLG 195
           RRRK HV PKREE E+PIV+ERKQ LAG    +WGV+D+ILVGGILIS FTI GLLFHLG
Sbjct: 568 RRRKSHVLPKREETENPIVTERKQTLAGGRLDEWGVVDKILVGGILISTFTIFGLLFHLG 627

Query: 196 STTLSSSW 199
           STT SSSW
Sbjct: 628 STTFSSSW 635

[Close]
BLAST of Spg021017 vs. ExPASy TrEMBL
Match: A0A1S3CA89 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis melo OX=3656 GN=LOC103498722 PE=3 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 3.4e-72
Identity = 147/188 (78.19%), Postives = 159/188 (84.57%), Query Frame = 0 

Query: 16  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRD 75
           VGHTKGYIQVLVIAPETMLGTSAM KITS+GRWSVFGEVIETISTKHHK TTLED L RD
Sbjct: 447 VGHTKGYIQVLVIAPETMLGTSAMAKITSIGRWSVFGEVIETISTKHHK-TTLEDTLTRD 506

Query: 76  EVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDSSRNIPLPEESKRKNLIGWLL 135
           +V PCSN HE CACS EP+ CAC   SCKG V++ D+ DSSRNIPLPEE KRKNLI W+L
Sbjct: 507 KVSPCSNTHETCACSTEPESCACELGSCKGAVSVGDEVDSSRNIPLPEEPKRKNLIEWVL 566

Query: 136 RRRKGHV-PKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLG 195
           RRRK  V PKREE E+PIV+ERKQ LAG    +WGV+D ILVGGILIS FTI GLLFH+G
Sbjct: 567 RRRKSQVMPKREETENPIVTERKQTLAGGRLDEWGVVDMILVGGILISTFTIFGLLFHVG 626

Query: 196 STTLSSSW 199
           STTLSSSW
Sbjct: 627 STTLSSSW 633

[Close]
BLAST of Spg021017 vs. ExPASy TrEMBL
Match: A0A1S3CAU3 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis melo OX=3656 GN=LOC103498722 PE=3 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 3.4e-72
Identity = 147/188 (78.19%), Postives = 159/188 (84.57%), Query Frame = 0 

Query: 16  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRD 75
           VGHTKGYIQVLVIAPETMLGTSAM KITS+GRWSVFGEVIETISTKHHK TTLED L RD
Sbjct: 391 VGHTKGYIQVLVIAPETMLGTSAMAKITSIGRWSVFGEVIETISTKHHK-TTLEDTLTRD 450

Query: 76  EVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDSSRNIPLPEESKRKNLIGWLL 135
           +V PCSN HE CACS EP+ CAC   SCKG V++ D+ DSSRNIPLPEE KRKNLI W+L
Sbjct: 451 KVSPCSNTHETCACSTEPESCACELGSCKGAVSVGDEVDSSRNIPLPEEPKRKNLIEWVL 510

Query: 136 RRRKGHV-PKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLG 195
           RRRK  V PKREE E+PIV+ERKQ LAG    +WGV+D ILVGGILIS FTI GLLFH+G
Sbjct: 511 RRRKSQVMPKREETENPIVTERKQTLAGGRLDEWGVVDMILVGGILISTFTIFGLLFHVG 570

Query: 196 STTLSSSW 199
           STTLSSSW
Sbjct: 571 STTLSSSW 577

[Close]
BLAST of Spg021017 vs. TAIR 10
Match: AT1G72090.1 (Methylthiotransferase )

HSP 1 Score: 107.1 bits (266), Expect = 1.7e-23
Identity = 73/179 (40.78%), Postives = 100/179 (55.87%), Query Frame = 0 

Query: 16  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRD 75
           VGHTKGY+QVLV  PE+MLGTSAM +ITSVGRWSVFGEVIET S+ + +T + E+  +  
Sbjct: 448 VGHTKGYVQVLVTGPESMLGTSAMARITSVGRWSVFGEVIETFSSANRETKSREETKL-- 507

Query: 76  EVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDSSRNIPLPEESKRKNLIGWLL 135
              PCS+    C      + C C  ESC G     +  + S NI   +++K K+      
Sbjct: 508 ---PCSSNVSTC------ETCTCSAESC-GEERSGEACNISGNISGQDDNKGKS------ 567

Query: 136 RRRKGHVPKREERESPIVSERKQALAGDWGVIDRILVGGILISIFTIVGLLFHLGSTTL 195
                   K+EE+E   V     ++A +WG ID+ LV G+ +S  TI+ LL  +    L
Sbjct: 568 --------KKEEKEVQEVVVPGSSVA-NWGFIDKALVCGVFVSSVTILVLLISIACRVL 599

The following BLAST results are available for this feature:
BLAST of Spg021017 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038890156.17.2e-8587.70threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Benincasa hisp... [more]
XP_038890157.17.2e-8587.70threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2 [Benincasa hisp... [more]
XP_023524910.18.2e-8184.49threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita pepo subsp. pep... [more]
XP_022949069.13.1e-8083.96threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita moschata][more]
XP_022997808.11.0e-7882.89threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita maxima][more]
BLAST of Spg021017 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Spg021017 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1GBS11.5e-8083.96Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucurbita moschata OX=3... [more]
A0A6J1KEZ54.9e-7982.89Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucurbita maxima OX=366... [more]
A0A0A0KSZ41.2e-7478.19Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis sativus OX=3659... [more]
A0A1S3CA893.4e-7278.19Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis melo OX=3656 GN... [more]
A0A1S3CAU33.4e-7278.19Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis melo OX=3656 GN... [more]
BLAST of Spg021017 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G72090.11.7e-2340.78Methylthiotransferase [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg021017.1Spg021017.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035600 tRNA methylthiolation
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0035598 N6-threonylcarbomyladenosine methylthiotransferase activity
molecular_function GO:0061712 tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase