Spg020822 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg020822
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionLaccase
Locationscaffold10: 28833692 .. 28837126 (+)
RNA-Seq ExpressionSpg020822
SyntenySpg020822
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GATTATTCACCCACCAATTTTCAAAAAATTCCTTTTCTCTTGAGAAAAGCCATTAAAAATGGGCTTCTCTATTCTCAAATTATTCTCCATCCTCTGTTTCACTTGGTCATGTTTCATTCCTCAGCTGGTATTTGCAGGCATAACCAGGCATTACAAGTTTGAAGTATGTATTTTTCAACTTCTCTCTATTTTTTCTTTTACCCTTACTCGCTCGTTCTATACTTTGAATATAACTAATTTCATCTTTGAATTTCTTAGATTAAATTGCAAAATGTGACACGTTTGTGCCACACAAAGAGTATGGTGACAGTCAATGGCAAGTTTCCTGGGCCTCGTATAATTGCTAGGGAGGGTGATCGTCTTCTAATCAAAGTGGTTAACCATGTCTCTAACAACATGTCTATCCATTGGTAAAATTTACCATTTTGATGAAAAAATTATATAGCTACATTGAAGCAAAATATTACAAAAATAACATTATTCTTTATATATGTGCGAGGCAGGCATGGAATAAGACAACTTAGATCAGGCTGGGCTGATGGACCGGCATATATAACTCAATGTCCGATACAAACGGGAAAAAGCTATGTATATAATTACACCATTGTTGGACAAAGAGGAACTCTTTTCTGGCATGCTCATATTTCTTGGCTAAGATCAACCGTTTATGGGCCTATAATCATCCTTCCTAAACATGGAGTTCCTTACCCATTTACCAAACCCCACCGGGAGGTTCCAATCATCTTTGGTAACTTCTTAAATTTCAAGCAATTTTTCTCTTAACTAACATATTTTTTTTTTTTTTTTTTTAATTTCAAGCCATTAACAACGACCATTATTTCAATATTTTCAAATTGTACTTTTTTAGGAGAGTGGTGGAATGCAGATACTGAAGCAGTCATTAGCCAAGCACTGCAAACAGGTGGAGGTCCAAATGTTTCTGATGCTTATACAATGAATGGCCTTCCTGGACCTTTGTATAATTGCTCAACCAAAGGTAAATTTAATCAGATCTCTTATGTTATTCAAAAGAAAAAAAAAAAAAAAACAGATCTCTTATGTCATAAATCAATAAATTAATTAGGAACCCGATATGTTTAGTATGCTACCAAGTTTAAATCGTTAAATAATAAAAGCCAACATTTAATTTTGACAAAACTTTTTATGGTAGAGATTAAATTGTTACAAATTTGAAATTAAAAAGACTAATTGTTGCAAAATAAATTTGACGAACTAAATTGTTTTCCTTTCATGAAAATTTAGGAATCAAAAAATGTTTTTTTAACCTACCATTCTTCCTCTTACATTATAAAGTTTAAAATCATTTTTGGTCACAAAACTTTTGTGAAAGTAGCAATTTAGTCCTTGAACTTTGATTTGTAACTATTTAGTTTCTATACTTTCAATTTTGTGACAATTTAGTCTCTGTGTCCTATGTAACAATTCAGTCTCTGTCATTTAGAATTTGTAATGATTTAGTCCTTATCGTAAAAATTAATGTTAAGATTTAATCAAATCTCTCACATACATAGATTAATAAACTAATTTGGTACCCAATATATTTATAAGCTACAAAGCCTAATCATGAAACAATAAAAACCGAGATTTAATTTTGATGATTTTTTTTTTCAACAAGAATTAAATTATTACAAATTTGAGTTCAAAGACTAAATTGTTACAAAATTGAAAGTACATAACTAAATTATTACAAATCAAAGTTCATGAACTAAATTGTTAGTTTAGTGATTTTTAACCCATCATAAAAATACAATATTTTGATTAAATCTTATAAAATTTGGTGGAGCAGATACATTAAAATTGAAAGTGAAGGCAGGAAAAACATACATGCTTCGTCTGATCAATGCAGCATTAAACGACGAGCTTTTCTTCAGCATAGCAAATCACAGTCTCACAGTGGTCGAAGCCGACGCCGTCTATGTCAAACCCTTCAAAACCAACACCCTTTTGATTGCACCTGGACAGACCACCAACATCCTTCTTCACACCAAATCCCTCATCCCAAACGCCTCCTTTTACATGCTTGCACGACCCTACGCGACAGGCCAAGGAACATTCGACAACTCCACAGTTGCCGGAGTTCTCGAATACGACCAAATTTCCACCTCCAAAAACCTCCCACTCTACAAACCAACTCTTCCTGCACTGAACGACACTTCATTCGCAACAAATTTCACCAAAAAGCTTCGAAGCCTGGCCAGCTCTCAATTCCCAGCCAACGTCCCTCGGAAGGTCGACAAGCGATTTTTCTTTACTGTCGGCCTCGGGACGAACCCTTGTAGCCAAAAGAACCAAACTTGTCAAGGGCCGAATGGAACCATGTTTGCAGCTTCGGTGAACAATGTATCTTTTGTTATGCCAAACACGGCCTTGCTTCAGGCTCACTTCTTTGGGAAATCTAATGGGCTTTATACTACTGATTTTCCAAATAGCCCTCTAATTCCTTTCAATTACACGGGCAATAATCCTCCCAACAATACCATGGTTGGAAATGGGACAAAGCTGGTGGTGTTGCCTTTCAATGCTAGTGTGGAGCTGGTGATGCAAGATACGAGTATACTTGGAGCTGAGAGCCACCCTCTCCATTTGCATGGGTTTAATTTCTTTGTGGTTGGACAAGGATTTGGGAACTTTGATCCTAAAAATGACCCTGCAAATTTCAACCTTGTTGACCCTGTTGAAAGGAATACTGTGGGAGTGCCGTCCGGTGGATGGGTTGCTATTCGCTTCCAAGCGGATAATCCAGGTAAATCATCTCTTTCTGTTAAGGAAAAAACTTAACTGCATACCGGTATGTAGAAATCTCCCTGCATATCACCATTAAAATGTTGATAATTAGAAGAGATAAAAAAAATTTCTCATTTTCTCCATTCCTCTTTATTAGTTGTCAAGATTTTAATGGGGTATGTAGGGAGATCCCTACATACCGGTATGCAACCAAATTTTGTTCTTCTGTTAATATTCAATCATGTAATTGATTTATATATTTTAGATATTATTGATTGGTAATTGCCCTTTGTTTGTGGACAGGTGTATGGTTCATGCATTGCCACTTGGAGGTACACACGAGCTGGGGTTTGAAGATGGCATGGTTGGTATTGGATGGAAAGCTCCCTAATCAGAAGCTCTTGCCTCCCCCATCGGATCTTCCCAAATGTTGAAATTTTGCCCTGAGCATATTTTTGCTTGTTTTGTTTTTCAAAATTCTTTTGTTTTGATTGGCATTTTGGAAAATCTCAAGTCTTTTTAATTATTATTCCACCCTTATCGTGAGGCTCAGATTGAAGAGATGCCCTATGATTTGTTTTATTCTTTGCTACATGTTTAGCTTTTTTGCTTGTGTACTTTGGGATGGCAACTCTTCCCAAGAAAGATATAATATCTTGTAACATGGATTTATTTGTTCATTAACAAGAG

mRNA sequence

ATGAATGGCCTTCCTGGACCTTTGTATAATTGCTCAACCAAAGATACATTAAAATTGAAAGTGAAGGCAGGAAAAACATACATGCTTCGTCTGATCAATGCAGCATTAAACGACGAGCTTTTCTTCAGCATAGCAAATCACAGTCTCACAGTGGTCGAAGCCGACGCCGTCTATGTCAAACCCTTCAAAACCAACACCCTTTTGATTGCACCTGGACAGACCACCAACATCCTTCTTCACACCAAATCCCTCATCCCAAACGCCTCCTTTTACATGCTTGCACGACCCTACGCGACAGGCCAAGGAACATTCGACAACTCCACAGTTGCCGGAGTTCTCGAATACGACCAAATTTCCACCTCCAAAAACCTCCCACTCTACAAACCAACTCTTCCTGCACTGAACGACACTTCATTCGCAACAAATTTCACCAAAAAGCTTCGAAGCCTGGCCAGCTCTCAATTCCCAGCCAACGTCCCTCGGAAGGTCGACAAGCGATTTTTCTTTACTGTCGGCCTCGGGACGAACCCTTGTAGCCAAAAGAACCAAACTTGTCAAGGGCCGAATGGAACCATGTTTGCAGCTTCGGTGAACAATGTATCTTTTGTTATGCCAAACACGGCCTTGCTTCAGGCTCACTTCTTTGGGAAATCTAATGGGCTTTATACTACTGATTTTCCAAATAGCCCTCTAATTCCTTTCAATTACACGGGCAATAATCCTCCCAACAATACCATGGTTGGAAATGGGACAAAGCTGGTGGTGTTGCCTTTCAATGCTAGTGTGGAGCTGGTGATGCAAGATACGAGTATACTTGGAGCTGAGAGCCACCCTCTCCATTTGCATGGGTTTAATTTCTTTGTGGTTGGACAAGGATTTGGGAACTTTGATCCTAAAAATGACCCTGCAAATTTCAACCTTGTTGACCCTGTTGAAAGGAATACTGTGGGAGTGCCGTCCGGTGGATGGGTTGCTATTCGCTTCCAAGCGGATAATCCAGGTGTATGGTTCATGCATTGCCACTTGGAGGTACACACGAGCTGGGGTTTGAAGATGGCATGGTTGGTATTGGATGGAAAGCTCCCTAATCAGAAGCTCTTGCCTCCCCCATCGGATCTTCCCAAATGTTGA

Coding sequence (CDS)

ATGAATGGCCTTCCTGGACCTTTGTATAATTGCTCAACCAAAGATACATTAAAATTGAAAGTGAAGGCAGGAAAAACATACATGCTTCGTCTGATCAATGCAGCATTAAACGACGAGCTTTTCTTCAGCATAGCAAATCACAGTCTCACAGTGGTCGAAGCCGACGCCGTCTATGTCAAACCCTTCAAAACCAACACCCTTTTGATTGCACCTGGACAGACCACCAACATCCTTCTTCACACCAAATCCCTCATCCCAAACGCCTCCTTTTACATGCTTGCACGACCCTACGCGACAGGCCAAGGAACATTCGACAACTCCACAGTTGCCGGAGTTCTCGAATACGACCAAATTTCCACCTCCAAAAACCTCCCACTCTACAAACCAACTCTTCCTGCACTGAACGACACTTCATTCGCAACAAATTTCACCAAAAAGCTTCGAAGCCTGGCCAGCTCTCAATTCCCAGCCAACGTCCCTCGGAAGGTCGACAAGCGATTTTTCTTTACTGTCGGCCTCGGGACGAACCCTTGTAGCCAAAAGAACCAAACTTGTCAAGGGCCGAATGGAACCATGTTTGCAGCTTCGGTGAACAATGTATCTTTTGTTATGCCAAACACGGCCTTGCTTCAGGCTCACTTCTTTGGGAAATCTAATGGGCTTTATACTACTGATTTTCCAAATAGCCCTCTAATTCCTTTCAATTACACGGGCAATAATCCTCCCAACAATACCATGGTTGGAAATGGGACAAAGCTGGTGGTGTTGCCTTTCAATGCTAGTGTGGAGCTGGTGATGCAAGATACGAGTATACTTGGAGCTGAGAGCCACCCTCTCCATTTGCATGGGTTTAATTTCTTTGTGGTTGGACAAGGATTTGGGAACTTTGATCCTAAAAATGACCCTGCAAATTTCAACCTTGTTGACCCTGTTGAAAGGAATACTGTGGGAGTGCCGTCCGGTGGATGGGTTGCTATTCGCTTCCAAGCGGATAATCCAGGTGTATGGTTCATGCATTGCCACTTGGAGGTACACACGAGCTGGGGTTTGAAGATGGCATGGTTGGTATTGGATGGAAAGCTCCCTAATCAGAAGCTCTTGCCTCCCCCATCGGATCTTCCCAAATGTTGA

Protein sequence

MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVKPFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYDQISTSKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTNPCSQKNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFNYTGNNPPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPKNDPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWLVLDGKLPNQKLLPPPSDLPKC
Homology
BLAST of Spg020822 vs. NCBI nr
Match: XP_022964682.1 (laccase-17-like [Cucurbita moschata])

HSP 1 Score: 694.1 bits (1790), Expect = 6.6e-196
Identity = 325/376 (86.44%), Postives = 352/376 (93.62%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           MNGLPGPLYNCS KDTLKLKVK GKTY+LRLINAALNDELFFSIANH+LT+V+ DA+Y+K
Sbjct: 198 MNGLPGPLYNCSAKDTLKLKVKPGKTYLLRLINAALNDELFFSIANHTLTIVDVDAIYIK 257

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYDQIST 120
           PFKTNTLLIAPGQTTN+LLHTKS  PNASF M+ARPY TGQGTFDNSTVAG+LEY+ +  
Sbjct: 258 PFKTNTLLIAPGQTTNLLLHTKSHFPNASFLMVARPYVTGQGTFDNSTVAGILEYETLKH 317

Query: 121 SKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTNPCSQ 180
            KNLP++KP LPALNDTSFATN+TKKLRSLA+S+FPANVP+K+DK+FFFTVGLGTNPC+Q
Sbjct: 318 PKNLPVFKPNLPALNDTSFATNYTKKLRSLANSKFPANVPQKIDKKFFFTVGLGTNPCNQ 377

Query: 181 KNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFNYTGNN 240
           KNQTCQGPNGTMFAAS+NNVSFVMPN ALLQAH+ G+SNG+Y   FPN PLIPFNYTG +
Sbjct: 378 KNQTCQGPNGTMFAASINNVSFVMPNIALLQAHYKGQSNGVYVPFFPNDPLIPFNYTG-S 437

Query: 241 PPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPKN 300
           PPNNTMV NGTKLVVLPFN SVEL+MQDTSILGAESHPLHLHGFNFFVVGQGFGNFDP  
Sbjct: 438 PPNNTMVSNGTKLVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNK 497

Query: 301 DPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWLVLDGK 360
           DPANFNLVDPVERNTVGVPSGGWVAIRF ADNPGVWFMHCHLEVHTSWGLKMAWLVLDGK
Sbjct: 498 DPANFNLVDPVERNTVGVPSGGWVAIRFLADNPGVWFMHCHLEVHTSWGLKMAWLVLDGK 557

Query: 361 LPNQKLLPPPSDLPKC 377
           LPNQKLLPPP+DLPKC
Sbjct: 558 LPNQKLLPPPADLPKC 572

BLAST of Spg020822 vs. NCBI nr
Match: XP_022970415.1 (laccase-17-like [Cucurbita maxima])

HSP 1 Score: 693.0 bits (1787), Expect = 1.5e-195
Identity = 324/376 (86.17%), Postives = 350/376 (93.09%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           MNGLPGPLYNCS K+TLKLKVK GKTY+LRLINAALNDELFFSIANH+LT+V+ DA+Y+K
Sbjct: 198 MNGLPGPLYNCSAKETLKLKVKPGKTYLLRLINAALNDELFFSIANHTLTIVDVDAIYIK 257

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYDQIST 120
           PFKTNTLLIAPGQTTN+LLHTKS  PNASF MLARPY TGQGTFDNSTVAG+LEY  +  
Sbjct: 258 PFKTNTLLIAPGQTTNLLLHTKSHFPNASFLMLARPYVTGQGTFDNSTVAGILEYQTLKH 317

Query: 121 SKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTNPCSQ 180
            KNLPL+KP LPALNDTSFATN+TKKLRSLA+ +FPANVP+K+DK FFFTVGLGTNPC+Q
Sbjct: 318 QKNLPLFKPNLPALNDTSFATNYTKKLRSLANFEFPANVPQKIDKNFFFTVGLGTNPCNQ 377

Query: 181 KNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFNYTGNN 240
           KNQTCQGPNGTMFAAS+NNVSFVMPNTA+LQAH+ G+SNG+Y   FPN PLIPFNYTG +
Sbjct: 378 KNQTCQGPNGTMFAASINNVSFVMPNTAILQAHYKGQSNGVYVPFFPNDPLIPFNYTG-S 437

Query: 241 PPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPKN 300
           PPNNTMV NGTKLVVLPFN SVEL+MQDTSILGAESHPLHLHGFNFFVVGQGFGNFDP  
Sbjct: 438 PPNNTMVSNGTKLVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNK 497

Query: 301 DPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWLVLDGK 360
           DPANFNLVDP+ERNTVGVPSGGWVAIRF ADNPGVWFMHCHLEVHTSWGLKMAWLVLDGK
Sbjct: 498 DPANFNLVDPIERNTVGVPSGGWVAIRFLADNPGVWFMHCHLEVHTSWGLKMAWLVLDGK 557

Query: 361 LPNQKLLPPPSDLPKC 377
           LPNQKLLPPP+DLPKC
Sbjct: 558 LPNQKLLPPPADLPKC 572

BLAST of Spg020822 vs. NCBI nr
Match: XP_038894038.1 (laccase-17-like [Benincasa hispida])

HSP 1 Score: 692.6 bits (1786), Expect = 1.9e-195
Identity = 330/377 (87.53%), Postives = 352/377 (93.37%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           MNGLPGPLYNCSTKDTLKLKVK GKTY+LRLINAALNDELFFSIANHSLT+V+ DA+YVK
Sbjct: 198 MNGLPGPLYNCSTKDTLKLKVKPGKTYLLRLINAALNDELFFSIANHSLTIVDVDAIYVK 257

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLE-YDQIS 120
           PFKTNTL+IAPGQTTN+LL TKS  PNA F+M ARPY TGQGTFDNSTVAG+LE YDQIS
Sbjct: 258 PFKTNTLIIAPGQTTNVLLQTKSHFPNAKFFMFARPYVTGQGTFDNSTVAGILEYYDQIS 317

Query: 121 TSKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTNPCS 180
             KNLP+YKP LP LNDTSF TNFTKKLRSLA+SQFPANVP+KVDK+FFFTVGLGTNPC+
Sbjct: 318 -KKNLPIYKPNLPLLNDTSFVTNFTKKLRSLANSQFPANVPQKVDKQFFFTVGLGTNPCN 377

Query: 181 QKNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFNYTGN 240
           QKNQTCQGPNGTMFAAS+NNVSFVMPN ALLQAH+ G+SNG+Y+  FP SPLIPFNYTG 
Sbjct: 378 QKNQTCQGPNGTMFAASINNVSFVMPNIALLQAHYKGQSNGVYSPFFPTSPLIPFNYTG- 437

Query: 241 NPPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPK 300
           NPPNNTMVGNGTK+VVLPFN SVEL+MQDTSILGAESHPLHLHGFNFFVVGQGFGNFDP 
Sbjct: 438 NPPNNTMVGNGTKVVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPN 497

Query: 301 NDPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWLVLDG 360
            DP NFNLVDPVERNTVGVP+GGWVAIRF ADNPGVWFMHCHLEVHTSWGLKMAWLVLDG
Sbjct: 498 KDPKNFNLVDPVERNTVGVPAGGWVAIRFLADNPGVWFMHCHLEVHTSWGLKMAWLVLDG 557

Query: 361 KLPNQKLLPPPSDLPKC 377
           KLPNQKLLPPP+DLPKC
Sbjct: 558 KLPNQKLLPPPADLPKC 572

BLAST of Spg020822 vs. NCBI nr
Match: XP_023519282.1 (laccase-17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 690.6 bits (1781), Expect = 7.3e-195
Identity = 327/381 (85.83%), Postives = 352/381 (92.39%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           MNGLPGPLYNCS KDTLKLKVK GKTY+LRLINAALNDELFFSIANH+LT+V+ DA+Y+K
Sbjct: 198 MNGLPGPLYNCSAKDTLKLKVKPGKTYLLRLINAALNDELFFSIANHTLTIVDVDAIYIK 257

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYDQIST 120
           PFKTNTLLIAPGQTTN+LLHTKS  PNASF MLARPY TGQGTFDNSTVAG+LEY+ +  
Sbjct: 258 PFKTNTLLIAPGQTTNLLLHTKSHFPNASFLMLARPYVTGQGTFDNSTVAGILEYETLKH 317

Query: 121 SKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDK-----RFFFTVGLGT 180
            KNLPL+KP LPALNDTSFATN+TKKLRSLA+S+FPANVP+K+DK     +FFFTVGLGT
Sbjct: 318 PKNLPLFKPNLPALNDTSFATNYTKKLRSLANSKFPANVPQKIDKKFFFTKFFFTVGLGT 377

Query: 181 NPCSQKNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFN 240
           NPC+QKNQTCQGPNGTMFAAS+NNVSFVMPNTALLQAH+ G+SNG+Y   FPN PLIPFN
Sbjct: 378 NPCNQKNQTCQGPNGTMFAASINNVSFVMPNTALLQAHYKGQSNGVYVPFFPNDPLIPFN 437

Query: 241 YTGNNPPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGN 300
           YTG +PPNNTMV NGTKLVVLPFN SVEL+MQDTSILGAESHPLHLHGFNFFVVGQGFGN
Sbjct: 438 YTG-SPPNNTMVSNGTKLVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGN 497

Query: 301 FDPKNDPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWL 360
           FDP  DP NFNLVDPVERNTVGVPSGGWVAIRF ADNPGVWFMHCHLEVHTSWGLKMAWL
Sbjct: 498 FDPNKDPPNFNLVDPVERNTVGVPSGGWVAIRFLADNPGVWFMHCHLEVHTSWGLKMAWL 557

Query: 361 VLDGKLPNQKLLPPPSDLPKC 377
           VLDGKLPNQKLLPPP+DLPKC
Sbjct: 558 VLDGKLPNQKLLPPPADLPKC 577

BLAST of Spg020822 vs. NCBI nr
Match: XP_004148786.1 (laccase-17 [Cucumis sativus] >KGN64741.1 hypothetical protein Csa_013745 [Cucumis sativus])

HSP 1 Score: 686.4 bits (1770), Expect = 1.4e-193
Identity = 325/381 (85.30%), Postives = 352/381 (92.39%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           MNGLPGP YNCSTKDTLKLKVK GKTY+LRLINAALNDELFFSIANHSLT+V+ DAVY+K
Sbjct: 200 MNGLPGPFYNCSTKDTLKLKVKPGKTYLLRLINAALNDELFFSIANHSLTIVDVDAVYIK 259

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYD---Q 120
           PFKTNTL+IAPGQT+N+LL TKS  PNA FYMLARPY TGQGTFDNSTVAG+LEYD   Q
Sbjct: 260 PFKTNTLIIAPGQTSNVLLQTKSNFPNAKFYMLARPYVTGQGTFDNSTVAGILEYDDHQQ 319

Query: 121 ISTSKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTNP 180
            +  K LP+YKP LP+LNDTSF TNFTKKLRSLA+SQFPANVP+K+DK+FFFTVGLGTNP
Sbjct: 320 KNPKKTLPIYKPNLPSLNDTSFVTNFTKKLRSLANSQFPANVPQKIDKKFFFTVGLGTNP 379

Query: 181 CS--QKNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFN 240
           CS  +KNQTCQGPNGTMFAAS+NNVSF+MPN ALLQ+H+ G+SNG+Y+  FPNSPLIPFN
Sbjct: 380 CSNNKKNQTCQGPNGTMFAASINNVSFIMPNVALLQSHYKGQSNGVYSPFFPNSPLIPFN 439

Query: 241 YTGNNPPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGN 300
           YTG NPPNNTMVGNGTK+VVLPFN SVEL+MQDTSILGAESHPLHLHGFNFFVVGQGFGN
Sbjct: 440 YTG-NPPNNTMVGNGTKVVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGN 499

Query: 301 FDPKNDPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWL 360
           FDP  DP NFNLVDPVERNTVGVP+GGWVAIRF ADNPGVWFMHCHLEVHTSWGLKMAWL
Sbjct: 500 FDPNKDPQNFNLVDPVERNTVGVPAGGWVAIRFLADNPGVWFMHCHLEVHTSWGLKMAWL 559

Query: 361 VLDGKLPNQKLLPPPSDLPKC 377
           VLDGKLPNQKLLPPP+DLPKC
Sbjct: 560 VLDGKLPNQKLLPPPADLPKC 579

BLAST of Spg020822 vs. ExPASy Swiss-Prot
Match: Q9FJD5 (Laccase-17 OS=Arabidopsis thaliana OX=3702 GN=LAC17 PE=2 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 7.8e-176
Identity = 295/387 (76.23%), Postives = 334/387 (86.30%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           +NGLPGPLYNCS KDT +L+VK GKTY+LRLINAALNDELFFSIANH++TVVEADA+YVK
Sbjct: 192 INGLPGPLYNCSAKDTFRLRVKPGKTYLLRLINAALNDELFFSIANHTVTVVEADAIYVK 251

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYD---- 120
           PF+T T+LIAPGQTTN+LL TKS  P+ASF+M ARPY TGQGTFDNSTVAG+LEY+    
Sbjct: 252 PFETETILIAPGQTTNVLLKTKSSYPSASFFMTARPYVTGQGTFDNSTVAGILEYEPPKQ 311

Query: 121 -----QISTSKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTV 180
                  ++ KNL L+KP LPALNDT+FAT F+ KLRSL S  FPANVP  VD++FFFTV
Sbjct: 312 TKGAHSRTSIKNLQLFKPILPALNDTNFATKFSNKLRSLNSKNFPANVPLNVDRKFFFTV 371

Query: 181 GLGTNPCSQK-NQTCQGP-NGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNS 240
           GLGTNPC+ K NQTCQGP N TMFAAS++N+SF MP  ALLQ+H+ G+S+G+Y+  FP S
Sbjct: 372 GLGTNPCNHKNNQTCQGPTNTTMFAASISNISFTMPTKALLQSHYSGQSHGVYSPKFPWS 431

Query: 241 PLIPFNYTGNNPPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVV 300
           P++PFNYTG  PPNNTMV NGT L+VLP+N SVELVMQDTSILGAESHPLHLHGFNFFVV
Sbjct: 432 PIVPFNYTG-TPPNNTMVSNGTNLMVLPYNTSVELVMQDTSILGAESHPLHLHGFNFFVV 491

Query: 301 GQGFGNFDPKNDPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWG 360
           GQGFGNFDP  DP NFNLVDP+ERNTVGVPSGGW AIRF ADNPGVWFMHCHLEVHTSWG
Sbjct: 492 GQGFGNFDPNKDPRNFNLVDPIERNTVGVPSGGWAAIRFLADNPGVWFMHCHLEVHTSWG 551

Query: 361 LKMAWLVLDGKLPNQKLLPPPSDLPKC 377
           L+MAWLVLDG  P+QKLLPPP+DLPKC
Sbjct: 552 LRMAWLVLDGDKPDQKLLPPPADLPKC 577

BLAST of Spg020822 vs. ExPASy Swiss-Prot
Match: B9FJH4 (Laccase-12 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC12 PE=3 SV=2)

HSP 1 Score: 596.7 bits (1537), Expect = 1.9e-169
Identity = 287/380 (75.53%), Postives = 324/380 (85.26%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           +NGLPGPLYNCS +DT KLKVK GKTYMLRLINAALNDELFFSIANH+LTVV+ DA+YVK
Sbjct: 197 LNGLPGPLYNCSAQDTFKLKVKPGKTYMLRLINAALNDELFFSIANHTLTVVDVDALYVK 256

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYDQ--- 120
           PF  +TL+IAPGQT+N+LL  K   P AS+YMLARPY T QGTFDN+TVAGVLEYD    
Sbjct: 257 PFTVDTLIIAPGQTSNVLLTAKPTYPGASYYMLARPYTTTQGTFDNTTVAGVLEYDDPCP 316

Query: 121 -ISTSKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTN 180
             +  K +P++ PTLP +NDT+  +NFT KLRSLAS+ +PA VP++VD RFFFTVGLGT+
Sbjct: 317 TTAAGKIVPIFSPTLPQINDTNAVSNFTAKLRSLASAGYPAAVPQQVDHRFFFTVGLGTH 376

Query: 181 PCSQKNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFNY 240
           PC+  N TCQGPNG+ FAAS+NNVSFV+P TALLQ+HF GKS G+Y ++FP  PL PFNY
Sbjct: 377 PCA-VNGTCQGPNGSRFAASINNVSFVLPATALLQSHFAGKSKGVYASNFPYYPLNPFNY 436

Query: 241 TGNNPPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNF 300
           TG  PPNNT V NGTK++VLP+ A+VELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNF
Sbjct: 437 TG-TPPNNTNVMNGTKVLVLPYGANVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNF 496

Query: 301 DPKNDPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWLV 360
           DP NDPA FNL DPVERNTVGVP+GGWVAIRF ADNPGVWFMHCHLEVH SWGLKMAWLV
Sbjct: 497 DPINDPAKFNLYDPVERNTVGVPAGGWVAIRFHADNPGVWFMHCHLEVHMSWGLKMAWLV 556

Query: 361 LDGKLPNQKLLPPPSDLPKC 377
           LDG  P+QKL PPP DLPKC
Sbjct: 557 LDGSRPDQKLPPPPLDLPKC 574

BLAST of Spg020822 vs. ExPASy Swiss-Prot
Match: P0DKK6 (Laccase-13 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC13 PE=2 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 1.9e-169
Identity = 287/380 (75.53%), Postives = 324/380 (85.26%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           +NGLPGPLYNCS +DT KLKVK GKTYMLRLINAALNDELFFSIANH+LTVV+ DA+YVK
Sbjct: 197 LNGLPGPLYNCSAQDTFKLKVKPGKTYMLRLINAALNDELFFSIANHTLTVVDVDALYVK 256

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYDQ--- 120
           PF  +TL+IAPGQT+N+LL  K   P AS+YMLARPY T QGTFDN+TVAGVLEYD    
Sbjct: 257 PFTVDTLIIAPGQTSNVLLTAKPTYPGASYYMLARPYTTTQGTFDNTTVAGVLEYDDPCP 316

Query: 121 -ISTSKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTN 180
             +  K +P++ PTLP +NDT+  +NFT KLRSLAS+ +PA VP++VD RFFFTVGLGT+
Sbjct: 317 TTAAGKIVPIFSPTLPQINDTNAVSNFTAKLRSLASAGYPAAVPQQVDHRFFFTVGLGTH 376

Query: 181 PCSQKNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFNY 240
           PC+  N TCQGPNG+ FAAS+NNVSFV+P TALLQ+HF GKS G+Y ++FP  PL PFNY
Sbjct: 377 PCA-VNGTCQGPNGSRFAASINNVSFVLPATALLQSHFAGKSKGVYASNFPYYPLNPFNY 436

Query: 241 TGNNPPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNF 300
           TG  PPNNT V NGTK++VLP+ A+VELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNF
Sbjct: 437 TG-TPPNNTNVMNGTKVLVLPYGANVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNF 496

Query: 301 DPKNDPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWLV 360
           DP NDPA FNL DPVERNTVGVP+GGWVAIRF ADNPGVWFMHCHLEVH SWGLKMAWLV
Sbjct: 497 DPINDPAKFNLYDPVERNTVGVPAGGWVAIRFHADNPGVWFMHCHLEVHMSWGLKMAWLV 556

Query: 361 LDGKLPNQKLLPPPSDLPKC 377
           LDG  P+QKL PPP DLPKC
Sbjct: 557 LDGSRPDQKLPPPPLDLPKC 574

BLAST of Spg020822 vs. ExPASy Swiss-Prot
Match: O81081 (Laccase-2 OS=Arabidopsis thaliana OX=3702 GN=LAC2 PE=2 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 2.5e-166
Identity = 276/378 (73.02%), Postives = 322/378 (85.19%), Query Frame = 0

Query: 2   NGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVKP 61
           NGLPGPLYNCSTKDT KL VK GKTY+LRLINAALNDELFF+IANH+LTVVEADA YVKP
Sbjct: 198 NGLPGPLYNCSTKDTYKLMVKPGKTYLLRLINAALNDELFFTIANHTLTVVEADACYVKP 257

Query: 62  FKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYD-QIST 121
           F+TN +L+ PGQTTN+LL TK + PNA+FYMLARPY TGQGT DN+TVAG+L+Y     +
Sbjct: 258 FQTNIVLLGPGQTTNVLLKTKPIYPNATFYMLARPYFTGQGTIDNTTVAGILQYQHHTKS 317

Query: 122 SKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTNPCSQ 181
           SKNL + KP+LP +N TS+A NFTK  RSLASS FPANVP+ VDK++FF +GLGTNPC  
Sbjct: 318 SKNLSIIKPSLPPINSTSYAANFTKMFRSLASSTFPANVPKVVDKQYFFAIGLGTNPC-P 377

Query: 182 KNQTCQGP-NGTMFAASVNNVSFVMPN-TALLQAHFFGKSNGLYTTDFPNSPLIPFNYTG 241
           KNQTCQGP N T FAAS+NNVSF++PN T+LLQ++F GKS  ++ TDFP +P+IPFNYTG
Sbjct: 378 KNQTCQGPTNTTKFAASINNVSFILPNKTSLLQSYFVGKSKNVFMTDFPTAPIIPFNYTG 437

Query: 242 NNPPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDP 301
             PPNNTMV  GTK+VVL +  +VELV+Q TSILG E+HP+HLHGFNF+VVGQGFGNF+P
Sbjct: 438 -TPPNNTMVSRGTKVVVLKYKTTVELVLQGTSILGIEAHPIHLHGFNFYVVGQGFGNFNP 497

Query: 302 KNDPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWLVLD 361
             DP ++NLVDPVERNT+ +PSGGWVAIRF ADNPGVW MHCH+E+H SWGL MAW+VLD
Sbjct: 498 ARDPKHYNLVDPVERNTINIPSGGWVAIRFLADNPGVWLMHCHIEIHLSWGLTMAWVVLD 557

Query: 362 GKLPNQKLLPPPSDLPKC 377
           G LPNQKLLPPPSD PKC
Sbjct: 558 GDLPNQKLLPPPSDFPKC 573

BLAST of Spg020822 vs. ExPASy Swiss-Prot
Match: Q5N9X2 (Laccase-4 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC4 PE=2 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 2.8e-165
Identity = 283/384 (73.70%), Postives = 318/384 (82.81%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           +NGLPGPLYNCS +DT KLKVK GKTYMLRLINAALN+ELFF++ANH+LTVVE DAVYVK
Sbjct: 198 INGLPGPLYNCSAQDTFKLKVKPGKTYMLRLINAALNEELFFAVANHTLTVVEVDAVYVK 257

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQ-GTFDNSTVAGVLEYDQIS 120
           PF  +TL+I+PGQTTN+LL  K   P A+FYM A PY+T + GTF N+TVAG+LEY+  +
Sbjct: 258 PFTVDTLVISPGQTTNVLLTAKPYYPGANFYMSAAPYSTARPGTFGNTTVAGILEYENPA 317

Query: 121 TS-------KNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVG 180
            S       K LPL+KPTLP LNDT F TNFT KLRSLA+ ++PA VP+ VDKRFFFTVG
Sbjct: 318 MSPSAASFVKGLPLFKPTLPQLNDTDFVTNFTDKLRSLATPEYPAAVPQSVDKRFFFTVG 377

Query: 181 LGTNPCSQKNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLI 240
           LGT PC   N TCQGPN T  AAS+NNVSFV+P  ALLQ+HF G S+G+Y  DFP +PL 
Sbjct: 378 LGTLPC-PANMTCQGPNNTQMAASMNNVSFVLPARALLQSHFTGLSSGVYAPDFPVAPLS 437

Query: 241 PFNYTGNNPPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQG 300
           PFNYTG  PPNNT V  GTKL+VL +N SVELVMQDTSILG ESHPLHLHGFNFFV+GQG
Sbjct: 438 PFNYTG-TPPNNTNVKTGTKLLVLRYNTSVELVMQDTSILGIESHPLHLHGFNFFVIGQG 497

Query: 301 FGNFDPKNDPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKM 360
           FGN+D  NDPA FNLVDPVERNTVGVP+GGWVAIRF ADNPGVWFMHCHLE HT+WGL+M
Sbjct: 498 FGNYDAVNDPAKFNLVDPVERNTVGVPAGGWVAIRFLADNPGVWFMHCHLEAHTTWGLRM 557

Query: 361 AWLVLDGKLPNQKLLPPPSDLPKC 377
           AWLVLDG  PNQKLLPPPSDLPKC
Sbjct: 558 AWLVLDGSHPNQKLLPPPSDLPKC 579

BLAST of Spg020822 vs. ExPASy TrEMBL
Match: A0A6J1HIE5 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111464691 PE=3 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 3.2e-196
Identity = 325/376 (86.44%), Postives = 352/376 (93.62%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           MNGLPGPLYNCS KDTLKLKVK GKTY+LRLINAALNDELFFSIANH+LT+V+ DA+Y+K
Sbjct: 198 MNGLPGPLYNCSAKDTLKLKVKPGKTYLLRLINAALNDELFFSIANHTLTIVDVDAIYIK 257

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYDQIST 120
           PFKTNTLLIAPGQTTN+LLHTKS  PNASF M+ARPY TGQGTFDNSTVAG+LEY+ +  
Sbjct: 258 PFKTNTLLIAPGQTTNLLLHTKSHFPNASFLMVARPYVTGQGTFDNSTVAGILEYETLKH 317

Query: 121 SKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTNPCSQ 180
            KNLP++KP LPALNDTSFATN+TKKLRSLA+S+FPANVP+K+DK+FFFTVGLGTNPC+Q
Sbjct: 318 PKNLPVFKPNLPALNDTSFATNYTKKLRSLANSKFPANVPQKIDKKFFFTVGLGTNPCNQ 377

Query: 181 KNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFNYTGNN 240
           KNQTCQGPNGTMFAAS+NNVSFVMPN ALLQAH+ G+SNG+Y   FPN PLIPFNYTG +
Sbjct: 378 KNQTCQGPNGTMFAASINNVSFVMPNIALLQAHYKGQSNGVYVPFFPNDPLIPFNYTG-S 437

Query: 241 PPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPKN 300
           PPNNTMV NGTKLVVLPFN SVEL+MQDTSILGAESHPLHLHGFNFFVVGQGFGNFDP  
Sbjct: 438 PPNNTMVSNGTKLVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNK 497

Query: 301 DPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWLVLDGK 360
           DPANFNLVDPVERNTVGVPSGGWVAIRF ADNPGVWFMHCHLEVHTSWGLKMAWLVLDGK
Sbjct: 498 DPANFNLVDPVERNTVGVPSGGWVAIRFLADNPGVWFMHCHLEVHTSWGLKMAWLVLDGK 557

Query: 361 LPNQKLLPPPSDLPKC 377
           LPNQKLLPPP+DLPKC
Sbjct: 558 LPNQKLLPPPADLPKC 572

BLAST of Spg020822 vs. ExPASy TrEMBL
Match: A0A6J1I2T0 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111469405 PE=3 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 7.1e-196
Identity = 324/376 (86.17%), Postives = 350/376 (93.09%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           MNGLPGPLYNCS K+TLKLKVK GKTY+LRLINAALNDELFFSIANH+LT+V+ DA+Y+K
Sbjct: 198 MNGLPGPLYNCSAKETLKLKVKPGKTYLLRLINAALNDELFFSIANHTLTIVDVDAIYIK 257

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYDQIST 120
           PFKTNTLLIAPGQTTN+LLHTKS  PNASF MLARPY TGQGTFDNSTVAG+LEY  +  
Sbjct: 258 PFKTNTLLIAPGQTTNLLLHTKSHFPNASFLMLARPYVTGQGTFDNSTVAGILEYQTLKH 317

Query: 121 SKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTNPCSQ 180
            KNLPL+KP LPALNDTSFATN+TKKLRSLA+ +FPANVP+K+DK FFFTVGLGTNPC+Q
Sbjct: 318 QKNLPLFKPNLPALNDTSFATNYTKKLRSLANFEFPANVPQKIDKNFFFTVGLGTNPCNQ 377

Query: 181 KNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFNYTGNN 240
           KNQTCQGPNGTMFAAS+NNVSFVMPNTA+LQAH+ G+SNG+Y   FPN PLIPFNYTG +
Sbjct: 378 KNQTCQGPNGTMFAASINNVSFVMPNTAILQAHYKGQSNGVYVPFFPNDPLIPFNYTG-S 437

Query: 241 PPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPKN 300
           PPNNTMV NGTKLVVLPFN SVEL+MQDTSILGAESHPLHLHGFNFFVVGQGFGNFDP  
Sbjct: 438 PPNNTMVSNGTKLVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNK 497

Query: 301 DPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWLVLDGK 360
           DPANFNLVDP+ERNTVGVPSGGWVAIRF ADNPGVWFMHCHLEVHTSWGLKMAWLVLDGK
Sbjct: 498 DPANFNLVDPIERNTVGVPSGGWVAIRFLADNPGVWFMHCHLEVHTSWGLKMAWLVLDGK 557

Query: 361 LPNQKLLPPPSDLPKC 377
           LPNQKLLPPP+DLPKC
Sbjct: 558 LPNQKLLPPPADLPKC 572

BLAST of Spg020822 vs. ExPASy TrEMBL
Match: A0A0A0LXL0 (Laccase OS=Cucumis sativus OX=3659 GN=Csa_1G085370 PE=3 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 6.6e-194
Identity = 325/381 (85.30%), Postives = 352/381 (92.39%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           MNGLPGP YNCSTKDTLKLKVK GKTY+LRLINAALNDELFFSIANHSLT+V+ DAVY+K
Sbjct: 200 MNGLPGPFYNCSTKDTLKLKVKPGKTYLLRLINAALNDELFFSIANHSLTIVDVDAVYIK 259

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYD---Q 120
           PFKTNTL+IAPGQT+N+LL TKS  PNA FYMLARPY TGQGTFDNSTVAG+LEYD   Q
Sbjct: 260 PFKTNTLIIAPGQTSNVLLQTKSNFPNAKFYMLARPYVTGQGTFDNSTVAGILEYDDHQQ 319

Query: 121 ISTSKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTNP 180
            +  K LP+YKP LP+LNDTSF TNFTKKLRSLA+SQFPANVP+K+DK+FFFTVGLGTNP
Sbjct: 320 KNPKKTLPIYKPNLPSLNDTSFVTNFTKKLRSLANSQFPANVPQKIDKKFFFTVGLGTNP 379

Query: 181 CS--QKNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFN 240
           CS  +KNQTCQGPNGTMFAAS+NNVSF+MPN ALLQ+H+ G+SNG+Y+  FPNSPLIPFN
Sbjct: 380 CSNNKKNQTCQGPNGTMFAASINNVSFIMPNVALLQSHYKGQSNGVYSPFFPNSPLIPFN 439

Query: 241 YTGNNPPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGN 300
           YTG NPPNNTMVGNGTK+VVLPFN SVEL+MQDTSILGAESHPLHLHGFNFFVVGQGFGN
Sbjct: 440 YTG-NPPNNTMVGNGTKVVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGN 499

Query: 301 FDPKNDPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWL 360
           FDP  DP NFNLVDPVERNTVGVP+GGWVAIRF ADNPGVWFMHCHLEVHTSWGLKMAWL
Sbjct: 500 FDPNKDPQNFNLVDPVERNTVGVPAGGWVAIRFLADNPGVWFMHCHLEVHTSWGLKMAWL 559

Query: 361 VLDGKLPNQKLLPPPSDLPKC 377
           VLDGKLPNQKLLPPP+DLPKC
Sbjct: 560 VLDGKLPNQKLLPPPADLPKC 579

BLAST of Spg020822 vs. ExPASy TrEMBL
Match: A0A5A7ULP7 (Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold486G00400 PE=3 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 2.1e-192
Identity = 323/381 (84.78%), Postives = 350/381 (91.86%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           MNGLPGP YNCS KDTLKLKVK GKTY+LRLINAALNDELFFSIANHSLT+V+ DAVY+K
Sbjct: 187 MNGLPGPFYNCSAKDTLKLKVKPGKTYLLRLINAALNDELFFSIANHSLTIVDVDAVYIK 246

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYD---Q 120
           P KTNTL+IAPGQT+N+LL TKS  PNA FYMLARPY TGQGTFDNSTVAG+LEYD   Q
Sbjct: 247 PLKTNTLIIAPGQTSNVLLQTKSNFPNAKFYMLARPYVTGQGTFDNSTVAGILEYDGHQQ 306

Query: 121 ISTSKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTNP 180
            +  K LP+YKP LP+LNDTSF TNFTKKLRSLA+SQFPANVP+K+DK+FFFTVGLGTNP
Sbjct: 307 KNPKKTLPIYKPNLPSLNDTSFVTNFTKKLRSLANSQFPANVPQKIDKKFFFTVGLGTNP 366

Query: 181 C--SQKNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFN 240
           C  ++KNQTCQGPNGTMFAAS+NNVSFVMPN ALLQ+H+ G+SNG+Y+  FPNSPLIPFN
Sbjct: 367 CNNNKKNQTCQGPNGTMFAASINNVSFVMPNVALLQSHYKGQSNGVYSPFFPNSPLIPFN 426

Query: 241 YTGNNPPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGN 300
           YTG NPPNNTMVGNGTK+VVLPFN SVEL+MQDTSILGAESHPLHLHGFNFFVVGQGFGN
Sbjct: 427 YTG-NPPNNTMVGNGTKVVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGN 486

Query: 301 FDPKNDPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWL 360
           FDP  DP NFNLVDPVERNTVGVP+GGWVAIRF ADNPGVWFMHCHLEVHTSWGLKMAWL
Sbjct: 487 FDPNKDPQNFNLVDPVERNTVGVPAGGWVAIRFLADNPGVWFMHCHLEVHTSWGLKMAWL 546

Query: 361 VLDGKLPNQKLLPPPSDLPKC 377
           VLDGKLPNQKLLPPP+DLPKC
Sbjct: 547 VLDGKLPNQKLLPPPADLPKC 566

BLAST of Spg020822 vs. ExPASy TrEMBL
Match: A0A1S3CK53 (Laccase OS=Cucumis melo OX=3656 GN=LOC103501858 PE=3 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 2.1e-192
Identity = 323/381 (84.78%), Postives = 350/381 (91.86%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           MNGLPGP YNCS KDTLKLKVK GKTY+LRLINAALNDELFFSIANHSLT+V+ DAVY+K
Sbjct: 199 MNGLPGPFYNCSAKDTLKLKVKPGKTYLLRLINAALNDELFFSIANHSLTIVDVDAVYIK 258

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYD---Q 120
           P KTNTL+IAPGQT+N+LL TKS  PNA FYMLARPY TGQGTFDNSTVAG+LEYD   Q
Sbjct: 259 PLKTNTLIIAPGQTSNVLLQTKSNFPNAKFYMLARPYVTGQGTFDNSTVAGILEYDGHQQ 318

Query: 121 ISTSKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTNP 180
            +  K LP+YKP LP+LNDTSF TNFTKKLRSLA+SQFPANVP+K+DK+FFFTVGLGTNP
Sbjct: 319 KNPKKTLPIYKPNLPSLNDTSFVTNFTKKLRSLANSQFPANVPQKIDKKFFFTVGLGTNP 378

Query: 181 C--SQKNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFN 240
           C  ++KNQTCQGPNGTMFAAS+NNVSFVMPN ALLQ+H+ G+SNG+Y+  FPNSPLIPFN
Sbjct: 379 CNNNKKNQTCQGPNGTMFAASINNVSFVMPNVALLQSHYKGQSNGVYSPFFPNSPLIPFN 438

Query: 241 YTGNNPPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGN 300
           YTG NPPNNTMVGNGTK+VVLPFN SVEL+MQDTSILGAESHPLHLHGFNFFVVGQGFGN
Sbjct: 439 YTG-NPPNNTMVGNGTKVVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGN 498

Query: 301 FDPKNDPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWL 360
           FDP  DP NFNLVDPVERNTVGVP+GGWVAIRF ADNPGVWFMHCHLEVHTSWGLKMAWL
Sbjct: 499 FDPNKDPQNFNLVDPVERNTVGVPAGGWVAIRFLADNPGVWFMHCHLEVHTSWGLKMAWL 558

Query: 361 VLDGKLPNQKLLPPPSDLPKC 377
           VLDGKLPNQKLLPPP+DLPKC
Sbjct: 559 VLDGKLPNQKLLPPPADLPKC 578

BLAST of Spg020822 vs. TAIR 10
Match: AT5G60020.1 (laccase 17 )

HSP 1 Score: 617.8 bits (1592), Expect = 5.6e-177
Identity = 295/387 (76.23%), Postives = 334/387 (86.30%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           +NGLPGPLYNCS KDT +L+VK GKTY+LRLINAALNDELFFSIANH++TVVEADA+YVK
Sbjct: 192 INGLPGPLYNCSAKDTFRLRVKPGKTYLLRLINAALNDELFFSIANHTVTVVEADAIYVK 251

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYD---- 120
           PF+T T+LIAPGQTTN+LL TKS  P+ASF+M ARPY TGQGTFDNSTVAG+LEY+    
Sbjct: 252 PFETETILIAPGQTTNVLLKTKSSYPSASFFMTARPYVTGQGTFDNSTVAGILEYEPPKQ 311

Query: 121 -----QISTSKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTV 180
                  ++ KNL L+KP LPALNDT+FAT F+ KLRSL S  FPANVP  VD++FFFTV
Sbjct: 312 TKGAHSRTSIKNLQLFKPILPALNDTNFATKFSNKLRSLNSKNFPANVPLNVDRKFFFTV 371

Query: 181 GLGTNPCSQK-NQTCQGP-NGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNS 240
           GLGTNPC+ K NQTCQGP N TMFAAS++N+SF MP  ALLQ+H+ G+S+G+Y+  FP S
Sbjct: 372 GLGTNPCNHKNNQTCQGPTNTTMFAASISNISFTMPTKALLQSHYSGQSHGVYSPKFPWS 431

Query: 241 PLIPFNYTGNNPPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVV 300
           P++PFNYTG  PPNNTMV NGT L+VLP+N SVELVMQDTSILGAESHPLHLHGFNFFVV
Sbjct: 432 PIVPFNYTG-TPPNNTMVSNGTNLMVLPYNTSVELVMQDTSILGAESHPLHLHGFNFFVV 491

Query: 301 GQGFGNFDPKNDPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWG 360
           GQGFGNFDP  DP NFNLVDP+ERNTVGVPSGGW AIRF ADNPGVWFMHCHLEVHTSWG
Sbjct: 492 GQGFGNFDPNKDPRNFNLVDPIERNTVGVPSGGWAAIRFLADNPGVWFMHCHLEVHTSWG 551

Query: 361 LKMAWLVLDGKLPNQKLLPPPSDLPKC 377
           L+MAWLVLDG  P+QKLLPPP+DLPKC
Sbjct: 552 LRMAWLVLDGDKPDQKLLPPPADLPKC 577

BLAST of Spg020822 vs. TAIR 10
Match: AT2G29130.1 (laccase 2 )

HSP 1 Score: 586.3 bits (1510), Expect = 1.8e-167
Identity = 276/378 (73.02%), Postives = 322/378 (85.19%), Query Frame = 0

Query: 2   NGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVKP 61
           NGLPGPLYNCSTKDT KL VK GKTY+LRLINAALNDELFF+IANH+LTVVEADA YVKP
Sbjct: 198 NGLPGPLYNCSTKDTYKLMVKPGKTYLLRLINAALNDELFFTIANHTLTVVEADACYVKP 257

Query: 62  FKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYD-QIST 121
           F+TN +L+ PGQTTN+LL TK + PNA+FYMLARPY TGQGT DN+TVAG+L+Y     +
Sbjct: 258 FQTNIVLLGPGQTTNVLLKTKPIYPNATFYMLARPYFTGQGTIDNTTVAGILQYQHHTKS 317

Query: 122 SKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTNPCSQ 181
           SKNL + KP+LP +N TS+A NFTK  RSLASS FPANVP+ VDK++FF +GLGTNPC  
Sbjct: 318 SKNLSIIKPSLPPINSTSYAANFTKMFRSLASSTFPANVPKVVDKQYFFAIGLGTNPC-P 377

Query: 182 KNQTCQGP-NGTMFAASVNNVSFVMPN-TALLQAHFFGKSNGLYTTDFPNSPLIPFNYTG 241
           KNQTCQGP N T FAAS+NNVSF++PN T+LLQ++F GKS  ++ TDFP +P+IPFNYTG
Sbjct: 378 KNQTCQGPTNTTKFAASINNVSFILPNKTSLLQSYFVGKSKNVFMTDFPTAPIIPFNYTG 437

Query: 242 NNPPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDP 301
             PPNNTMV  GTK+VVL +  +VELV+Q TSILG E+HP+HLHGFNF+VVGQGFGNF+P
Sbjct: 438 -TPPNNTMVSRGTKVVVLKYKTTVELVLQGTSILGIEAHPIHLHGFNFYVVGQGFGNFNP 497

Query: 302 KNDPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWLVLD 361
             DP ++NLVDPVERNT+ +PSGGWVAIRF ADNPGVW MHCH+E+H SWGL MAW+VLD
Sbjct: 498 ARDPKHYNLVDPVERNTINIPSGGWVAIRFLADNPGVWLMHCHIEIHLSWGLTMAWVVLD 557

Query: 362 GKLPNQKLLPPPSDLPKC 377
           G LPNQKLLPPPSD PKC
Sbjct: 558 GDLPNQKLLPPPSDFPKC 573

BLAST of Spg020822 vs. TAIR 10
Match: AT5G03260.1 (laccase 11 )

HSP 1 Score: 434.1 bits (1115), Expect = 1.1e-121
Identity = 215/376 (57.18%), Postives = 269/376 (71.54%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           +NG PGPL+ CS K T  ++ +AGKTY+LR+INAALNDELFF IA H++TVVE DAVY K
Sbjct: 193 INGKPGPLFPCSEKHTFVIEAEAGKTYLLRIINAALNDELFFGIAGHNMTVVEIDAVYTK 252

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYDQIST 120
           PF T  +L+ PGQTTN+L+ T    PN  ++M A P+     + DN TV  +L+Y  +  
Sbjct: 253 PFTTKAILLGPGQTTNVLVKT-DRSPN-RYFMAASPFMDAPVSVDNKTVTAILQYKGVPN 312

Query: 121 SKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTNPCSQ 180
           +  LP+  P LP  NDTSFA ++  KL+SL +  FPA VP KVD+R F+T+GLG N C  
Sbjct: 313 TV-LPIL-PKLPLPNDTSFALDYNGKLKSLNTPNFPALVPLKVDRRLFYTIGLGINAC-- 372

Query: 181 KNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFNYTGNN 240
              TC   NGT  AAS+NN++F+MP TALL+AH +   +G++ TDFP+ P   FNYTG  
Sbjct: 373 --PTC--VNGTNLAASINNITFIMPKTALLKAH-YSNISGVFRTDFPDRPPKAFNYTGVP 432

Query: 241 PPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPKN 300
              N     GT+L  + FN ++ELV+QDT++L  ESHP HLHG+NFFVVG G GNFDPK 
Sbjct: 433 LTANLGTSTGTRLSRVKFNTTIELVLQDTNLLTVESHPFHLHGYNFFVVGTGVGNFDPKK 492

Query: 301 DPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWLVLDGK 360
           DPA FNLVDP ERNTVGVP+GGW AIRF+ADNPGVWFMHCHLEVHT WGLKMA++V +G+
Sbjct: 493 DPAKFNLVDPPERNTVGVPTGGWAAIRFRADNPGVWFMHCHLEVHTMWGLKMAFVVENGE 552

Query: 361 LPNQKLLPPPSDLPKC 377
            P   +LPPP D P C
Sbjct: 553 TPELSVLPPPKDYPSC 557

BLAST of Spg020822 vs. TAIR 10
Match: AT2G38080.1 (Laccase/Diphenol oxidase family protein )

HSP 1 Score: 433.0 bits (1112), Expect = 2.5e-121
Identity = 216/376 (57.45%), Postives = 264/376 (70.21%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           +NG PGP+ NC ++   KL V+ GKTY+LRL+NAALN+ELFF +A H  TVVE DAVYVK
Sbjct: 194 INGHPGPVRNCPSQG-YKLSVENGKTYLLRLVNAALNEELFFKVAGHIFTVVEVDAVYVK 253

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEYDQIST 120
           PFKT+T+LIAPGQTTN+LL          + + A P+       DN T    + Y    +
Sbjct: 254 PFKTDTVLIAPGQTTNVLLTASK--SAGKYLVTASPFMDAPIAVDNVTATATVHYSGTLS 313

Query: 121 SKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTNPCSQ 180
           S    L  P  P  N TS A NFT  LRSL S ++PA VP  +D   FFTVGLG N C  
Sbjct: 314 SSPTILTLP--PPQNATSIANNFTNSLRSLNSKKYPALVPTTIDHHLFFTVGLGLNAC-- 373

Query: 181 KNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFNYTGNN 240
              TC+  NG+   AS+NNV+F+MP TALL AH+F  S G++TTDFP +P   FNY+G +
Sbjct: 374 --PTCKAGNGSRVVASINNVTFIMPKTALLPAHYFNTS-GVFTTDFPKNPPHVFNYSGGS 433

Query: 241 PPNNTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPKN 300
              N     GT+L  LP+NA+V+LV+QDT ++  E+HP+HLHGFNFF VG+G GNF+   
Sbjct: 434 -VTNMATETGTRLYKLPYNATVQLVLQDTGVIAPENHPVHLHGFNFFEVGRGLGNFNSTK 493

Query: 301 DPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWLVLDGK 360
           DP NFNLVDPVERNT+GVPSGGWV IRF+ADNPGVWFMHCHLEVHT+WGLKMA+LV +GK
Sbjct: 494 DPKNFNLVDPVERNTIGVPSGGWVVIRFRADNPGVWFMHCHLEVHTTWGLKMAFLVENGK 553

Query: 361 LPNQKLLPPPSDLPKC 377
            PNQ +LPPP DLPKC
Sbjct: 554 GPNQSILPPPKDLPKC 558

BLAST of Spg020822 vs. TAIR 10
Match: AT5G58910.1 (laccase 16 )

HSP 1 Score: 414.1 bits (1063), Expect = 1.2e-115
Identity = 212/381 (55.64%), Postives = 267/381 (70.08%), Query Frame = 0

Query: 1   MNGLPGPLYNCSTKDTLKLKVKAGKTYMLRLINAALNDELFFSIANHSLTVVEADAVYVK 60
           +NG  G + NC ++ +  L V+AGKTYMLR+INAALN+ELFF IA H LTVVE DAVY K
Sbjct: 153 INGHSGSISNCPSQSSYGLPVRAGKTYMLRIINAALNEELFFKIAGHVLTVVEVDAVYTK 212

Query: 61  PFKTNTLLIAPGQTTNILLHTKSLIPNASFYMLARPYATGQGTFDNSTVAGVLEY----D 120
           P+KT+T+ IAPGQTTN+LL T +    +++ + A  +      +DN T    L Y     
Sbjct: 213 PYKTDTVFIAPGQTTNVLL-TANANAGSNYMVAATTFTDAHIPYDNVTATATLHYIGHTS 272

Query: 121 QISTSKNLPLYKPTLPALNDTSFATNFTKKLRSLASSQFPANVPRKVDKRFFFTVGLGTN 180
            +STSK   L   +LP  N T  AT FT+ LRSL S ++PA VP  V+   FFTVGLG N
Sbjct: 273 TVSTSKKTVL--ASLPPQNATWVATKFTRSLRSLNSLEYPARVPTTVEHSLFFTVGLGAN 332

Query: 181 PCSQKNQTCQGPNGTMFAASVNNVSFVMPNTALLQAHFFGKSNGLYTTDFPNSPLIPFNY 240
           PC    Q+C   NG    A +NNV+F MP TALLQAHFF  S G++T DFP  P  P++Y
Sbjct: 333 PC----QSCN--NGVRLVAGINNVTFTMPKTALLQAHFFNIS-GVFTDDFPAKPSNPYDY 392

Query: 241 TGNNPPN-NTMVGNGTKLVVLPFNASVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGN 300
           T       N     GTKL  LP+NA+V++V+Q+T+++ +++HP HLHGFNFF VG+G GN
Sbjct: 393 TAPVKLGVNAATMKGTKLYRLPYNATVQIVLQNTAMILSDNHPFHLHGFNFFEVGRGLGN 452

Query: 301 FDPKNDPANFNLVDPVERNTVGVPSGGWVAIRFQADNPGVWFMHCHLEVHTSWGLKMAWL 360
           F+P+ DP  FNLVDPVERNTVGVP+GGW AIRF ADNPGVWFMHCHLE+HT+WGLKMA++
Sbjct: 453 FNPEKDPKAFNLVDPVERNTVGVPAGGWTAIRFIADNPGVWFMHCHLELHTTWGLKMAFV 512

Query: 361 VLDGKLPNQKLLPPPSDLPKC 377
           V +G  P+Q LLPPP+DLPKC
Sbjct: 513 VDNGHGPDQSLLPPPADLPKC 523

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022964682.16.6e-19686.44laccase-17-like [Cucurbita moschata][more]
XP_022970415.11.5e-19586.17laccase-17-like [Cucurbita maxima][more]
XP_038894038.11.9e-19587.53laccase-17-like [Benincasa hispida][more]
XP_023519282.17.3e-19585.83laccase-17 [Cucurbita pepo subsp. pepo][more]
XP_004148786.11.4e-19385.30laccase-17 [Cucumis sativus] >KGN64741.1 hypothetical protein Csa_013745 [Cucumi... [more]
Match NameE-valueIdentityDescription
Q9FJD57.8e-17676.23Laccase-17 OS=Arabidopsis thaliana OX=3702 GN=LAC17 PE=2 SV=1[more]
B9FJH41.9e-16975.53Laccase-12 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC12 PE=3 SV=2[more]
P0DKK61.9e-16975.53Laccase-13 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC13 PE=2 SV=1[more]
O810812.5e-16673.02Laccase-2 OS=Arabidopsis thaliana OX=3702 GN=LAC2 PE=2 SV=1[more]
Q5N9X22.8e-16573.70Laccase-4 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HIE53.2e-19686.44Laccase OS=Cucurbita moschata OX=3662 GN=LOC111464691 PE=3 SV=1[more]
A0A6J1I2T07.1e-19686.17Laccase OS=Cucurbita maxima OX=3661 GN=LOC111469405 PE=3 SV=1[more]
A0A0A0LXL06.6e-19485.30Laccase OS=Cucumis sativus OX=3659 GN=Csa_1G085370 PE=3 SV=1[more]
A0A5A7ULP72.1e-19284.78Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold486G00400 PE=3 S... [more]
A0A1S3CK532.1e-19284.78Laccase OS=Cucumis melo OX=3656 GN=LOC103501858 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G60020.15.6e-17776.23laccase 17 [more]
AT2G29130.11.8e-16773.02laccase 2 [more]
AT5G03260.11.1e-12157.18laccase 11 [more]
AT2G38080.12.5e-12157.45Laccase/Diphenol oxidase family protein [more]
AT5G58910.11.2e-11555.64laccase 16 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 233..358
e-value: 8.0E-41
score: 139.0
IPR008972CupredoxinGENE3D2.60.40.420coord: 1..153
e-value: 1.1E-36
score: 128.8
IPR008972CupredoxinGENE3D2.60.40.420coord: 177..376
e-value: 2.0E-61
score: 209.3
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 1..152
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 159..376
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 1..116
e-value: 3.5E-32
score: 111.7
IPR017761LaccaseTIGRFAMTIGR03389TIGR03389coord: 1..376
e-value: 4.4E-181
score: 601.3
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 334..376
coord: 1..333
NoneNo IPR availablePANTHERPTHR11709:SF402LACCASEcoord: 334..376
coord: 1..333
IPR033138Multicopper oxidases, conserved sitePROSITEPS00079MULTICOPPER_OXIDASE1coord: 334..354
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 339..350
IPR034289Laccase, third cupredoxin domainCDDcd13897CuRO_3_LCC_plantcoord: 222..359
e-value: 2.76056E-87
score: 258.343
IPR034285Laccase, second cupredoxin domainCDDcd13875CuRO_2_LCC_plantcoord: 2..115
e-value: 6.03566E-62
score: 193.969

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg020822.1Spg020822.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046274 lignin catabolic process
cellular_component GO:0048046 apoplast
molecular_function GO:0005507 copper ion binding
molecular_function GO:0052716 hydroquinone:oxygen oxidoreductase activity
molecular_function GO:0016491 oxidoreductase activity