Spg020616 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg020616
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	E3 ubiquitin-protein ligase RNF170
Location	scaffold9: 16707026 .. 16707682 (-)
RNA-Seq Expression	Spg020616
Synteny	Spg020616

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAAGCCGTTGTACTTGTTCGTCGTCCTTTATAAATTATAACTGCTGTAATTTTTTTTTACAGATGGTACTAGGTGTAGTCTATGCTGTGAGTCCAATCGATATCATACCCGAAGGTATTAAATCTTTTCCTCTAGATACCTATAGCAAATATCAACATTGTATTGTAATTATTGGTTCCCTTATGGGTTTGTTTTTCCTTTTGGCTTGAATGTTTATGTGCAGCATTACTAGGATTATTTGGACTGATGGATGATATCTTTATACTGCTCATCTGCTTCCTTTATATTGCTACTATATATCGATCGGTACTCTATAATCGCCATGGGGGAACTTGAGTTGTCTTTGGCGCCTTGCCATGAACTGTAAGAAATTAGAGTAGCCATTTTTTATGTTCTTAGCTTTGTAGTTTTTCTGGAGGGAAACGTTTTGGCTTCATAAATTTCGTTTCCGTCTTGTTTTACTTGTAAAGCCAACTCTCCTTTTGTATTCTTTCTTGTATATGAAGTTCAAATGTGTTGTTTCATTTTGTGTTTGGGAATGTGAAGCTTCAGAACCACAAACATTATGTTGGGAAAATAGCCATCGGAGATCCCCGTGTGGTTGTCATCTGTGTTGCAAACTATAATTGAGGATGTGTTTGGAATGACTTTTC

mRNA sequence

ATGTCAAGCCGTTGTACTTGTTCGTCGTCCTTTATAAATTATAACTGCTGTAATTTTTTTTTACAGATGGTACTAGGTGTAGTCTATGCTGTGAGTCCAATCGATATCATACCCGAAGCATTACTAGGATTATTTGGACTGATGGATGATATCTTTATACTGCTCATCTGCTTCCTTTATATTGCTACTATATATCGATCGGTACTCTATAATCGCCATGGGGGAACTTGA

Coding sequence (CDS)

ATGTCAAGCCGTTGTACTTGTTCGTCGTCCTTTATAAATTATAACTGCTGTAATTTTTTTTTACAGATGGTACTAGGTGTAGTCTATGCTGTGAGTCCAATCGATATCATACCCGAAGCATTACTAGGATTATTTGGACTGATGGATGATATCTTTATACTGCTCATCTGCTTCCTTTATATTGCTACTATATATCGATCGGTACTCTATAATCGCCATGGGGGAACTTGA

Protein sequence

MSSRCTCSSSFINYNCCNFFLQMVLGVVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNRHGGT

Homology

[Close]

BLAST of Spg020616 vs. NCBI nr
Match: XP_038884720.1 (E3 ubiquitin-protein ligase RNF170-like isoform X2 [Benincasa hispida] >XP_038884721.1 E3 ubiquitin-protein ligase RNF170-like isoform X2 [Benincasa hispida])

HSP 1 Score: 102.4 bits (254), Expect = 1.7e-18
Identity = 52/57 (91.23%), Postives = 53/57 (92.98%), Query Frame = 0


Query: 20  FLQMVLGVVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNRHGGT 77
           +  MVL VVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIA IYRSVLYNRHGGT
Sbjct: 129 YTAMVLSVVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIAAIYRSVLYNRHGGT 185

[Close]

BLAST of Spg020616 vs. NCBI nr
Match: XP_008463070.1 (PREDICTED: E3 ubiquitin-protein ligase RNF170-like [Cucumis melo])

HSP 1 Score: 98.6 bits (244), Expect = 2.5e-17
Identity = 49/57 (85.96%), Postives = 52/57 (91.23%), Query Frame = 0


Query: 20  FLQMVLGVVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNRHGGT 77
           ++ MVL VVY VSPIDIIPEALLGLFGLMDDI ILLICFLYIA +YRSVLYNRHGGT
Sbjct: 129 YIAMVLSVVYTVSPIDIIPEALLGLFGLMDDILILLICFLYIAAMYRSVLYNRHGGT 185

[Close]

BLAST of Spg020616 vs. NCBI nr
Match: XP_031743956.1 (E3 ubiquitin-protein ligase RNF170 isoform X5 [Cucumis sativus] >KAE8646587.1 hypothetical protein Csa_005542 [Cucumis sativus])

HSP 1 Score: 98.2 bits (243), Expect = 3.2e-17
Identity = 48/57 (84.21%), Postives = 52/57 (91.23%), Query Frame = 0


Query: 20  FLQMVLGVVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNRHGGT 77
           ++ MVL V+Y VSPIDIIPEALLGLFGLMDDI ILLICFLYIA +YRSVLYNRHGGT
Sbjct: 129 YIAMVLSVIYTVSPIDIIPEALLGLFGLMDDILILLICFLYIAAMYRSVLYNRHGGT 185

[Close]

BLAST of Spg020616 vs. NCBI nr
Match: XP_022932121.1 (E3 ubiquitin-protein ligase RNF170-like [Cucurbita moschata] >XP_023524452.1 E3 ubiquitin-protein ligase RNF170-like isoform X1 [Cucurbita pepo subsp. pepo] >KAG6607576.1 E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 94.0 bits (232), Expect = 6.1e-16
Identity = 46/57 (80.70%), Postives = 50/57 (87.72%), Query Frame = 0


Query: 20  FLQMVLGVVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNRHGGT 77
           +  M L VVY VSPIDIIPEALLGLFGLMDD FILLICFLY+A +YRSVL+NRHGGT
Sbjct: 129 YTAMFLSVVYTVSPIDIIPEALLGLFGLMDDAFILLICFLYVAALYRSVLHNRHGGT 185

[Close]

BLAST of Spg020616 vs. NCBI nr
Match: KAG7037201.1 (E3 ubiquitin-protein ligase-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 94.0 bits (232), Expect = 6.1e-16
Identity = 46/57 (80.70%), Postives = 50/57 (87.72%), Query Frame = 0


Query: 20  FLQMVLGVVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNRHGGT 77
           +  M L VVY VSPIDIIPEALLGLFGLMDD FILLICFLY+A +YRSVL+NRHGGT
Sbjct: 126 YTAMFLSVVYTVSPIDIIPEALLGLFGLMDDAFILLICFLYVAALYRSVLHNRHGGT 182

[Close]

BLAST of Spg020616 vs. ExPASy Swiss-Prot
Match: Q7SZN2 (E3 ubiquitin-protein ligase RNF170 OS=Danio rerio OX=7955 GN=rnf170 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 9.1e-07
Identity = 23/49 (46.94%), Postives = 35/49 (71.43%), Query Frame = 0


Query: 27  VVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNRHGG 76
           + Y VSP+D +PE +LGL G +DD F++L+ F+YI+ +YR V+  R  G
Sbjct: 218 LAYLVSPLDFLPEGVLGLLGFLDDFFVILLLFIYISIMYREVVTQRLAG 266

[Close]

BLAST of Spg020616 vs. ExPASy Swiss-Prot
Match: Q5PPX5 (E3 ubiquitin-protein ligase RNF170 OS=Xenopus laevis OX=8355 GN=rnf170 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.6e-06
Identity = 23/46 (50.00%), Postives = 35/46 (76.09%), Query Frame = 0


Query: 27  VVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNR 73
           ++Y VSP+DIIPEAL G+ G +DD+F+L +  +YI+ +YR V+  R
Sbjct: 209 LLYLVSPLDIIPEALFGILGFLDDLFVLFLLLIYISIMYREVVTQR 254

[Close]

BLAST of Spg020616 vs. ExPASy Swiss-Prot
Match: Q28DS3 (E3 ubiquitin-protein ligase RNF170 OS=Xenopus tropicalis OX=8364 GN=rnf170 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 4.5e-06
Identity = 23/44 (52.27%), Postives = 32/44 (72.73%), Query Frame = 0


Query: 29  YAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNR 73
           Y VSP+DIIPEA+ GL G +DD F+L +  +YI+ +YR V+  R
Sbjct: 211 YLVSPLDIIPEAVFGLLGFLDDFFVLFLLLIYISIMYREVVTQR 254

[Close]

BLAST of Spg020616 vs. ExPASy Swiss-Prot
Match: F1MK05 (E3 ubiquitin-protein ligase RNF170 OS=Bos taurus OX=9913 GN=RNF170 PE=3 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 2.2e-05
Identity = 19/44 (43.18%), Postives = 31/44 (70.45%), Query Frame = 0


Query: 29  YAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNR 73
           Y +SP+D +PEAL G+ G +DD F++ +  +YI+ +YR V+  R
Sbjct: 213 YLISPLDFVPEALFGILGFLDDFFVIFLLLIYISIMYREVITQR 256

[Close]

BLAST of Spg020616 vs. ExPASy Swiss-Prot
Match: Q96K19 (E3 ubiquitin-protein ligase RNF170 OS=Homo sapiens OX=9606 GN=RNF170 PE=1 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 2.2e-05
Identity = 19/44 (43.18%), Postives = 31/44 (70.45%), Query Frame = 0


Query: 29  YAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNR 73
           Y +SP+D +PEAL G+ G +DD F++ +  +YI+ +YR V+  R
Sbjct: 212 YLISPLDFVPEALFGILGFLDDFFVIFLLLIYISIMYREVITQR 255

[Close]

BLAST of Spg020616 vs. ExPASy TrEMBL
Match: A0A1S3CIS2 (E3 ubiquitin-protein ligase RNF170 OS=Cucumis melo OX=3656 GN=LOC103501307 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.2e-17
Identity = 49/57 (85.96%), Postives = 52/57 (91.23%), Query Frame = 0


Query: 20  FLQMVLGVVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNRHGGT 77
           ++ MVL VVY VSPIDIIPEALLGLFGLMDDI ILLICFLYIA +YRSVLYNRHGGT
Sbjct: 129 YIAMVLSVVYTVSPIDIIPEALLGLFGLMDDILILLICFLYIAAMYRSVLYNRHGGT 185

[Close]

BLAST of Spg020616 vs. ExPASy TrEMBL
Match: A0A6J1I9X5 (E3 ubiquitin-protein ligase RNF170 OS=Cucurbita maxima OX=3661 GN=LOC111471404 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.9e-16
Identity = 46/57 (80.70%), Postives = 50/57 (87.72%), Query Frame = 0


Query: 20  FLQMVLGVVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNRHGGT 77
           +  M L VVY VSPIDIIPEALLGLFGLMDD FILLICFLY+A +YRSVL+NRHGGT
Sbjct: 129 YTAMFLSVVYTVSPIDIIPEALLGLFGLMDDAFILLICFLYVAALYRSVLHNRHGGT 185

[Close]

BLAST of Spg020616 vs. ExPASy TrEMBL
Match: A0A6J1EVH0 (E3 ubiquitin-protein ligase RNF170 OS=Cucurbita moschata OX=3662 GN=LOC111438431 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.9e-16
Identity = 46/57 (80.70%), Postives = 50/57 (87.72%), Query Frame = 0


Query: 20  FLQMVLGVVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNRHGGT 77
           +  M L VVY VSPIDIIPEALLGLFGLMDD FILLICFLY+A +YRSVL+NRHGGT
Sbjct: 129 YTAMFLSVVYTVSPIDIIPEALLGLFGLMDDAFILLICFLYVAALYRSVLHNRHGGT 185

[Close]

BLAST of Spg020616 vs. ExPASy TrEMBL
Match: A0A6J1DFH0 (E3 ubiquitin-protein ligase RNF170 OS=Momordica charantia OX=3673 GN=LOC111019617 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 5.0e-16
Identity = 45/57 (78.95%), Postives = 52/57 (91.23%), Query Frame = 0


Query: 20  FLQMVLGVVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNRHGGT 77
           ++ MVL VVY +SPIDIIPEA+LG+FGLMDDIFILLICFLYIA +YRSVLY RHGG+
Sbjct: 129 YIAMVLSVVYTLSPIDIIPEAMLGVFGLMDDIFILLICFLYIAAMYRSVLYYRHGGS 185

[Close]

BLAST of Spg020616 vs. ExPASy TrEMBL
Match: A0A6A5PAC4 (RING-type E3 ubiquitin transferase OS=Lupinus albus OX=3870 GN=Lal_00027159 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.0e-13
Identity = 37/57 (64.91%), Postives = 50/57 (87.72%), Query Frame = 0


Query: 20 FLQMVLGVVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNRHGGT 77
          F+QM+L  VY +SPIDIIPE +LG+ GL+DD+ I+LICFL++AT+YRSVL+ RHGG+
Sbjct: 10 FMQMILSAVYVISPIDIIPEGILGIVGLLDDLLIVLICFLHVATLYRSVLFRRHGGS 66

[Close]

BLAST of Spg020616 vs. TAIR 10
Match: AT1G22510.1 (RING/U-box protein with domain of unknown function (DUF 1232) )

HSP 1 Score: 79.0 bits (193), Expect = 1.9e-15
Identity = 33/57 (57.89%), Postives = 48/57 (84.21%), Query Frame = 0


Query: 20  FLQMVLGVVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNRHGGT 77
           ++ ++L  +Y +SPIDIIPE +LG+ GL+DD+ I LICFL++A +YRSVLY+RHGG+
Sbjct: 129 YIALILSAIYIISPIDIIPEGVLGVIGLLDDLLIALICFLHVAALYRSVLYSRHGGS 185

[Close]

BLAST of Spg020616 vs. TAIR 10
Match: AT1G22510.2 (RING/U-box protein with domain of unknown function (DUF 1232) )

HSP 1 Score: 79.0 bits (193), Expect = 1.9e-15
Identity = 33/57 (57.89%), Postives = 48/57 (84.21%), Query Frame = 0


Query: 20  FLQMVLGVVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNRHGGT 77
           ++ ++L  +Y +SPIDIIPE +LG+ GL+DD+ I LICFL++A +YRSVLY+RHGG+
Sbjct: 147 YIALILSAIYIISPIDIIPEGVLGVIGLLDDLLIALICFLHVAALYRSVLYSRHGGS 203

[Close]

BLAST of Spg020616 vs. TAIR 10
Match: AT1G72175.1 (RING/U-box protein with domain of unknown function (DUF 1232) )

HSP 1 Score: 76.3 bits (186), Expect = 1.2e-14
Identity = 34/57 (59.65%), Postives = 46/57 (80.70%), Query Frame = 0


Query: 20  FLQMVLGVVYAVSPIDIIPEALLGLFGLMDDIFILLICFLYIATIYRSVLYNRHGGT 77
           ++ M+L  VY +SPIDIIPE +LG+ GL+DD+ I LICFL++A +YRSVLY RH G+
Sbjct: 129 YIAMILSAVYIISPIDIIPEGVLGVVGLLDDLLIALICFLHVAALYRSVLYFRHAGS 185

The following BLAST results are available for this feature:

BLAST of Spg020616 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884720.1	1.7e-18	91.23	E3 ubiquitin-protein ligase RNF170-like isoform X2 [Benincasa hispida] >XP_03888...	[more]
XP_008463070.1	2.5e-17	85.96	PREDICTED: E3 ubiquitin-protein ligase RNF170-like [Cucumis melo]	[more]
XP_031743956.1	3.2e-17	84.21	E3 ubiquitin-protein ligase RNF170 isoform X5 [Cucumis sativus] >KAE8646587.1 hy...	[more]
XP_022932121.1	6.1e-16	80.70	E3 ubiquitin-protein ligase RNF170-like [Cucurbita moschata] >XP_023524452.1 E3 ...	[more]
KAG7037201.1	6.1e-16	80.70	E3 ubiquitin-protein ligase-like protein [Cucurbita argyrosperma subsp. argyrosp...	[more]

BLAST of Spg020616 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7SZN2	9.1e-07	46.94	E3 ubiquitin-protein ligase RNF170 OS=Danio rerio OX=7955 GN=rnf170 PE=2 SV=1	[more]
Q5PPX5	1.6e-06	50.00	E3 ubiquitin-protein ligase RNF170 OS=Xenopus laevis OX=8355 GN=rnf170 PE=2 SV=1	[more]
Q28DS3	4.5e-06	52.27	E3 ubiquitin-protein ligase RNF170 OS=Xenopus tropicalis OX=8364 GN=rnf170 PE=2 ...	[more]
F1MK05	2.2e-05	43.18	E3 ubiquitin-protein ligase RNF170 OS=Bos taurus OX=9913 GN=RNF170 PE=3 SV=2	[more]
Q96K19	2.2e-05	43.18	E3 ubiquitin-protein ligase RNF170 OS=Homo sapiens OX=9606 GN=RNF170 PE=1 SV=2	[more]

BLAST of Spg020616 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CIS2	1.2e-17	85.96	E3 ubiquitin-protein ligase RNF170 OS=Cucumis melo OX=3656 GN=LOC103501307 PE=4 ...	[more]
A0A6J1I9X5	2.9e-16	80.70	E3 ubiquitin-protein ligase RNF170 OS=Cucurbita maxima OX=3661 GN=LOC111471404 P...	[more]
A0A6J1EVH0	2.9e-16	80.70	E3 ubiquitin-protein ligase RNF170 OS=Cucurbita moschata OX=3662 GN=LOC111438431...	[more]
A0A6J1DFH0	5.0e-16	78.95	E3 ubiquitin-protein ligase RNF170 OS=Momordica charantia OX=3673 GN=LOC11101961...	[more]
A0A6A5PAC4	1.0e-13	64.91	RING-type E3 ubiquitin transferase OS=Lupinus albus OX=3870 GN=Lal_00027159 PE=4...	[more]

BLAST of Spg020616 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G22510.1	1.9e-15	57.89	RING/U-box protein with domain of unknown function (DUF 1232)	[more]
AT1G22510.2	1.9e-15	57.89	RING/U-box protein with domain of unknown function (DUF 1232)	[more]
AT1G72175.1	1.2e-14	59.65	RING/U-box protein with domain of unknown function (DUF 1232)	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010652	Domain of unknown function DUF1232	PFAM	PF06803	DUF1232	coord: 23..55 e-value: 5.4E-10 score: 38.9
None	No IPR available	PANTHER	PTHR22894:SF10	BNAC07G13660D PROTEIN	coord: 19..75
IPR038896	E3 ubiquitin-protein ligase RNF170	PANTHER	PTHR22894	UNCHARACTERIZED	coord: 19..75

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg020616.1	Spg020616.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0061630	ubiquitin protein ligase activity