Spg019223 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg019223
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionFarnesylcysteine lyase
Locationscaffold1: 44286500 .. 44287724 (+)
RNA-Seq ExpressionSpg019223
SyntenySpg019223
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGATCTTAATTTCCTCAAAAAAAAAAAAAAATAATAATAAGACATTGATCTTAAATAAAATCAAAATTCAAACCACAATTCATTTCTCAAGAAAAAAAAAAAAACCACAATTCAATCAGCCCCATTTGAGAAACAAAAAAGTTTCATCGTGTTTCTTTCAAACCCATCCATTCAAAACATGTACACGATCTTATCAAAAAAAAAAAAAAAAAAAAACATGTACACGGTTCCTTGGTCAATGATCTTCACTCTATTCCTCTTCTTCCTCATCGTTCCGCCGGCGACTTCCCAACCACCGCCGCCCACTGTATGTATTATAGGCAGCGGCATCGGCGGCTCCGCCGTCTCCCACTTCCTCCGGCAATATGCTCCATCGTACAACGCCTCCACCGATTTCAACATTCGGATATTCGAGCGCCACAACATTGTTGGCGGCCGAATGGCCACCGTCAACGTTGCCGGAGACACATTCGAGGCCGGAGCATCCATTTTGCACCCCAAGAATCTTCACGCCTTGAGATTCACTAAGCTACTCAATCTCACTGTCAAGAAACCCTCCTCTTCCGATTCGTTCTCTCTCGGCATTTGGGACGGCCATAATTTCGTCTTCAGAACCCTCAAGAGCAGCCTCACCTCCAAGGTTCCTTTTCTGGATAAGATCGTGGAGCTATGGAATCAGCTCGTCATGTTCCTTCGATACGGCTTCTCGCTTCTCAGAATGGAGGAATTTGTCGAGGTTCTTATCTTCTTCTGCGTTGATTTGATCTGGTTCTCTAAAATGCCCTGCCGTTGACTCTTTATTTTTGATGAATTGGTGATGCAGAGTGCTGTGGAAAGATTCTCGAAGTACTATGAAGGCTTTGAATCTAGACCTATCTTTGAGACTGTTAATGAGATGCTCAATTGGTCTGGTTTGTATAACTTCACCACTCGAACTTTGTATGAGGAATTAATTGATGCTCGGTTGTCTCGCTTGCTAATACAAGAGCTTGTCACTGTAAGAGGACTTCCATTTCTTGTTTGCACGAATCTCTATAAATTCATCTCAGTTGCATTTTCGAATCACTCTTTTTCATTTTTTTTGGCTGTCTATGGTGATTTGTTATTGCTGGGGATTGGGGATTGCTTCTCTGTTTTTCTTATATAAGGATAACCCTGAACGTGAAAACTTCATCTGTGTGCAAGCTGGTATATCTTGGTGTGAGTTTCTGAATGAAATTTGA

mRNA sequence

ATGTACACGATCTTATCAAAAAAAAAAAAAAAAAAAAACATGTACACGGTTCCTTGGTCAATGATCTTCACTCTATTCCTCTTCTTCCTCATCGTTCCGCCGGCGACTTCCCAACCACCGCCGCCCACTGTATGTATTATAGGCAGCGGCATCGGCGGCTCCGCCGTCTCCCACTTCCTCCGGCAATATGCTCCATCGTACAACGCCTCCACCGATTTCAACATTCGGATATTCGAGCGCCACAACATTGTTGGCGGCCGAATGGCCACCGTCAACGTTGCCGGAGACACATTCGAGGCCGGAGCATCCATTTTGCACCCCAAGAATCTTCACGCCTTGAGATTCACTAAGCTACTCAATCTCACTGTCAAGAAACCCTCCTCTTCCGATTCGTTCTCTCTCGGCATTTGGGACGGCCATAATTTCGTCTTCAGAACCCTCAAGAGCAGCCTCACCTCCAAGGTTCCTTTTCTGGATAAGATCGTGGAGCTATGGAATCAGCTCGTCATGTTCCTTCGATACGGCTTCTCGCTTCTCAGAATGGAGGAATTTGTCGAGAGTGCTGTGGAAAGATTCTCGAAGTACTATGAAGGCTTTGAATCTAGACCTATCTTTGAGACTGTTAATGAGATGCTCAATTGGTCTGGTTTGTATAACTTCACCACTCGAACTTTGTATGAGGAATTAATTGATGCTCGGTTGTCTCGCTTGCTAATACAAGAGCTTGTCACTGATAACCCTGAACGTGAAAACTTCATCTGTGTGCAAGCTGGTATATCTTGGTGTGAGTTTCTGAATGAAATTTGA

Coding sequence (CDS)

ATGTACACGATCTTATCAAAAAAAAAAAAAAAAAAAAACATGTACACGGTTCCTTGGTCAATGATCTTCACTCTATTCCTCTTCTTCCTCATCGTTCCGCCGGCGACTTCCCAACCACCGCCGCCCACTGTATGTATTATAGGCAGCGGCATCGGCGGCTCCGCCGTCTCCCACTTCCTCCGGCAATATGCTCCATCGTACAACGCCTCCACCGATTTCAACATTCGGATATTCGAGCGCCACAACATTGTTGGCGGCCGAATGGCCACCGTCAACGTTGCCGGAGACACATTCGAGGCCGGAGCATCCATTTTGCACCCCAAGAATCTTCACGCCTTGAGATTCACTAAGCTACTCAATCTCACTGTCAAGAAACCCTCCTCTTCCGATTCGTTCTCTCTCGGCATTTGGGACGGCCATAATTTCGTCTTCAGAACCCTCAAGAGCAGCCTCACCTCCAAGGTTCCTTTTCTGGATAAGATCGTGGAGCTATGGAATCAGCTCGTCATGTTCCTTCGATACGGCTTCTCGCTTCTCAGAATGGAGGAATTTGTCGAGAGTGCTGTGGAAAGATTCTCGAAGTACTATGAAGGCTTTGAATCTAGACCTATCTTTGAGACTGTTAATGAGATGCTCAATTGGTCTGGTTTGTATAACTTCACCACTCGAACTTTGTATGAGGAATTAATTGATGCTCGGTTGTCTCGCTTGCTAATACAAGAGCTTGTCACTGATAACCCTGAACGTGAAAACTTCATCTGTGTGCAAGCTGGTATATCTTGGTGTGAGTTTCTGAATGAAATTTGA

Protein sequence

MYTILSKKKKKKNMYTVPWSMIFTLFLFFLIVPPATSQPPPPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDFNIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFSLGIWDGHNFVFRTLKSSLTSKVPFLDKIVELWNQLVMFLRYGFSLLRMEEFVESAVERFSKYYEGFESRPIFETVNEMLNWSGLYNFTTRTLYEELIDARLSRLLIQELVTDNPERENFICVQAGISWCEFLNEI
Homology
BLAST of Spg019223 vs. NCBI nr
Match: XP_038882019.1 (farnesylcysteine lyase [Benincasa hispida])

HSP 1 Score: 424.9 bits (1091), Expect = 5.3e-115
Identity = 210/231 (90.91%), Postives = 219/231 (94.81%), Query Frame = 0

Query: 14  MYTVPWSMIFTLFLFFLIVPPATSQPPPPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDF 73
           MY V WS+I +LFL FL VPPAT+QPPPPTVCIIGSGIGGS+VSHFLRQYAPSYNASTDF
Sbjct: 1   MYVVRWSIIVSLFLCFLTVPPATTQPPPPTVCIIGSGIGGSSVSHFLRQYAPSYNASTDF 60

Query: 74  NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS 133
           NIRIFERHNIVGGRMATVN+A DTFEAGASILHPKNLHALRFT+LLNLTVKKPSSS SFS
Sbjct: 61  NIRIFERHNIVGGRMATVNIASDTFEAGASILHPKNLHALRFTELLNLTVKKPSSSGSFS 120

Query: 134 LGIWDGHNFVFRTLKSSLTSKVPFLDKIVELWNQLVMFLRYGFSLLRMEEFVESAVERFS 193
           LGIWDGH FVFRTL S LTS VPFLDKI+ELWNQLVMFLRYGFSLLRMEEFVESAVERFS
Sbjct: 121 LGIWDGHKFVFRTLTSGLTSTVPFLDKILELWNQLVMFLRYGFSLLRMEEFVESAVERFS 180

Query: 194 KYYEGFESRPIFETVNEMLNWSGLYNFTTRTLYEELIDARLSRLLIQELVT 245
           KYYEGFESRP+FETVNEMLNWSGL+N TTRTLYEELIDARLSRLLIQELVT
Sbjct: 181 KYYEGFESRPVFETVNEMLNWSGLHNLTTRTLYEELIDARLSRLLIQELVT 231

BLAST of Spg019223 vs. NCBI nr
Match: TYK05432.1 (farnesylcysteine lyase [Cucumis melo var. makuwa])

HSP 1 Score: 415.6 bits (1067), Expect = 3.2e-112
Identity = 205/231 (88.74%), Postives = 213/231 (92.21%), Query Frame = 0

Query: 14  MYTVPWSMIFTLFLFFLIVPPATSQPPPPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDF 73
           MY V WS+I  LFL FL  PP TS+PPPPTVCIIGSGIGGS+VSHFLRQYAPSYN STDF
Sbjct: 1   MYMVRWSIILPLFLCFLAFPPVTSRPPPPTVCIIGSGIGGSSVSHFLRQYAPSYNVSTDF 60

Query: 74  NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS 133
           NIRIFERHNIVGGRMATVN+AGDTFEAGASILHPKN HALRFT+LLNLTVKKPSSS SFS
Sbjct: 61  NIRIFERHNIVGGRMATVNIAGDTFEAGASILHPKNFHALRFTELLNLTVKKPSSSGSFS 120

Query: 134 LGIWDGHNFVFRTLKSSLTSKVPFLDKIVELWNQLVMFLRYGFSLLRMEEFVESAVERFS 193
           LGIWDGH FVFRTL S LTS VPF+DKIVELWNQLVMFLRYGFSLLRMEEFVE AVE+FS
Sbjct: 121 LGIWDGHKFVFRTLTSGLTSTVPFVDKIVELWNQLVMFLRYGFSLLRMEEFVERAVEQFS 180

Query: 194 KYYEGFESRPIFETVNEMLNWSGLYNFTTRTLYEELIDARLSRLLIQELVT 245
           KYYEGFESRP+FETVNEMLNWSGLY  TTRTLYEELIDARLSRLLIQELVT
Sbjct: 181 KYYEGFESRPVFETVNEMLNWSGLYKLTTRTLYEELIDARLSRLLIQELVT 231

BLAST of Spg019223 vs. NCBI nr
Match: KAG6603171.1 (Farnesylcysteine lyase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033483.1 Farnesylcysteine lyase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 414.5 bits (1064), Expect = 7.2e-112
Identity = 205/231 (88.74%), Postives = 216/231 (93.51%), Query Frame = 0

Query: 14  MYTVPWSMIFTLFLFFLIVPPATSQPPPPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDF 73
           M  V WS+I  LFLFFL VPPA +Q PPPTVCIIGSGIGGS+V+HFLRQYAPSYNAS DF
Sbjct: 1   MMMVRWSIIVPLFLFFLSVPPAIAQQPPPTVCIIGSGIGGSSVAHFLRQYAPSYNASVDF 60

Query: 74  NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS 133
           NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS
Sbjct: 61  NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS 120

Query: 134 LGIWDGHNFVFRTLKSSLTSKVPFLDKIVELWNQLVMFLRYGFSLLRMEEFVESAVERFS 193
           LGIWDG  FVFRTLKS LTSK  +LDKIVELWNQ+VMF+RYGFSLLRME+FVESAVE+FS
Sbjct: 121 LGIWDGRKFVFRTLKSGLTSKFSYLDKIVELWNQVVMFIRYGFSLLRMEKFVESAVEKFS 180

Query: 194 KYYEGFESRPIFETVNEMLNWSGLYNFTTRTLYEELIDARLSRLLIQELVT 245
           KYYEGFESRP+FETVN+MLNWSGLYN TTRTLYEELIDARLSRLLIQELVT
Sbjct: 181 KYYEGFESRPVFETVNDMLNWSGLYNLTTRTLYEELIDARLSRLLIQELVT 231

BLAST of Spg019223 vs. NCBI nr
Match: XP_023545088.1 (farnesylcysteine lyase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 414.1 bits (1063), Expect = 9.3e-112
Identity = 204/231 (88.31%), Postives = 216/231 (93.51%), Query Frame = 0

Query: 14  MYTVPWSMIFTLFLFFLIVPPATSQPPPPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDF 73
           M  V WS+I  LFLFFL VPPA +Q PPPTVCIIGSGIGGS+++HFLRQYAPSYNAS DF
Sbjct: 1   MMMVRWSIIVPLFLFFLSVPPAIAQQPPPTVCIIGSGIGGSSLAHFLRQYAPSYNASVDF 60

Query: 74  NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS 133
           NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS
Sbjct: 61  NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS 120

Query: 134 LGIWDGHNFVFRTLKSSLTSKVPFLDKIVELWNQLVMFLRYGFSLLRMEEFVESAVERFS 193
           LGIWDG  FVFRTLKS LTS  P+LDKIVELWNQ+VMF+RYGFSLLRME+FVESAVE+FS
Sbjct: 121 LGIWDGRKFVFRTLKSGLTSMFPYLDKIVELWNQVVMFIRYGFSLLRMEKFVESAVEKFS 180

Query: 194 KYYEGFESRPIFETVNEMLNWSGLYNFTTRTLYEELIDARLSRLLIQELVT 245
           KYYEGFESRP+FETVN+MLNWSGLYN TTRTLYEELIDARLSRLLIQELVT
Sbjct: 181 KYYEGFESRPVFETVNDMLNWSGLYNLTTRTLYEELIDARLSRLLIQELVT 231

BLAST of Spg019223 vs. NCBI nr
Match: KAA0034879.1 (farnesylcysteine lyase [Cucumis melo var. makuwa])

HSP 1 Score: 413.7 bits (1062), Expect = 1.2e-111
Identity = 204/231 (88.31%), Postives = 213/231 (92.21%), Query Frame = 0

Query: 14  MYTVPWSMIFTLFLFFLIVPPATSQPPPPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDF 73
           MY V WS+I  LFL FL  PP TS+PPPPTVCIIGSGIGGS+VSHFLRQYAPSYN STDF
Sbjct: 1   MYMVRWSIIVPLFLCFLAFPPVTSRPPPPTVCIIGSGIGGSSVSHFLRQYAPSYNVSTDF 60

Query: 74  NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS 133
           NIRIFERHNIVGGRMATVN+AGDTFEAGASILHPKN HALRFT+LLNLTVKKPSSS SFS
Sbjct: 61  NIRIFERHNIVGGRMATVNIAGDTFEAGASILHPKNFHALRFTELLNLTVKKPSSSGSFS 120

Query: 134 LGIWDGHNFVFRTLKSSLTSKVPFLDKIVELWNQLVMFLRYGFSLLRMEEFVESAVERFS 193
           LGIWDGH FVFRTL S LTS VPF+DKIVELWNQLVMFLRYGFSLLRMEEFVE AVE+FS
Sbjct: 121 LGIWDGHKFVFRTLTSGLTSTVPFVDKIVELWNQLVMFLRYGFSLLRMEEFVERAVEQFS 180

Query: 194 KYYEGFESRPIFETVNEMLNWSGLYNFTTRTLYEELIDARLSRLLIQELVT 245
           KYYEGFESRP+FETVN+MLNWSGLY  TTRTLYEELIDARLSRLLIQELVT
Sbjct: 181 KYYEGFESRPVFETVNKMLNWSGLYKLTTRTLYEELIDARLSRLLIQELVT 231

BLAST of Spg019223 vs. ExPASy Swiss-Prot
Match: P57681 (Farnesylcysteine lyase OS=Arabidopsis thaliana OX=3702 GN=FLCY PE=1 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.5e-59
Identity = 121/205 (59.02%), Postives = 143/205 (69.76%), Query Frame = 0

Query: 41  PPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDFNIRIFERHNIVGGRMATVNVAGDTFEA 100
           P TVCI+GSGIGGS+V+HFLR Y+ S        I +FERH IVGGRM TV VAGDTFEA
Sbjct: 35  PATVCIVGSGIGGSSVAHFLRNYSVS-TGLNQAKILMFERHEIVGGRMRTVTVAGDTFEA 94

Query: 101 GASILHPKNLHALRFTKLLNLTVKKPSS-SDSFSLGIWDGHNFVFRTLKSSLTSKVPFLD 160
           G SILHPKN H   F +  NLTV+ P+   +S ++GIWDG  FV +T  S   +K PFLD
Sbjct: 95  GGSILHPKNYHVKDFVERFNLTVRLPTPIEESSAIGIWDGKRFVVKTFGSG--TKFPFLD 154

Query: 161 KIVELWNQLVMFLRYGFSLLRMEEFVESAVERFSKYYEGFESRPIFETVNEMLNWSGLYN 220
            IV   N L +FLRYG SLLRM  F+E+ V+ F KYYE  ESRPIF+ V  ML WSGLYN
Sbjct: 155 TIVSWVNDLYLFLRYGLSLLRMSSFIENTVDNFLKYYESLESRPIFDNVEGMLKWSGLYN 214

Query: 221 FTTRTLYEELIDARLSRLLIQELVT 245
            T  TL E+L +A+LS LL+ ELVT
Sbjct: 215 LTKLTLQEKLSEAQLSPLLVNELVT 236

BLAST of Spg019223 vs. ExPASy Swiss-Prot
Match: Q9UHG3 (Prenylcysteine oxidase 1 OS=Homo sapiens OX=9606 GN=PCYOX1 PE=1 SV=3)

HSP 1 Score: 106.7 bits (265), Expect = 4.2e-22
Identity = 74/212 (34.91%), Postives = 108/212 (50.94%), Query Frame = 0

Query: 35  ATSQPPPPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDFNIRIFERHNIVGGRMATVNVA 94
           A  + PP  + IIG+GIGG++ +++LRQ         D  I +FER   VGGR+AT+ V 
Sbjct: 28  AELRAPPDKIAIIGAGIGGTSAAYYLRQ-----KFGKDVKIDLFEREE-VGGRLATMMVQ 87

Query: 95  GDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFSLGIWDGHNFVFRTLKSSLTSK 154
           G  +EAG S++HP NLH  RF K L L+  + S      LGI++G   VF        S 
Sbjct: 88  GQEYEAGGSVIHPLNLHMKRFVKDLGLSAVQASGG---LLGIYNGETLVFE------ESN 147

Query: 155 VPFLDKIVELWNQLVMFLRYGFSLLRMEEFVESAVERFSKYYEGFESRPIFETVNEMLNW 214
              ++ I  +W       RYGF  LRM  +VE  +++F + Y        F +V ++L+ 
Sbjct: 148 WFIINVIKLVW-------RYGFQSLRMHMWVEDVLDKFMRIYRYQSHDYAFSSVEKLLHA 207

Query: 215 SGLYNF---TTRTLYEELIDARLSRLLIQELV 244
            G  +F     RTL E L  A  S   + E++
Sbjct: 208 LGGDDFLGMLNRTLLETLQKAGFSEKFLNEMI 217

BLAST of Spg019223 vs. ExPASy Swiss-Prot
Match: Q95KC9 (Prenylcysteine oxidase OS=Macaca fascicularis OX=9541 GN=PCYOX1 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 9.3e-22
Identity = 73/212 (34.43%), Postives = 108/212 (50.94%), Query Frame = 0

Query: 35  ATSQPPPPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDFNIRIFERHNIVGGRMATVNVA 94
           A  + PP  + +IG+GIGG++ +++LRQ         D  I +FER   VGGR+AT+ V 
Sbjct: 28  AELRAPPDKIAVIGAGIGGTSAAYYLRQ-----KFGKDVKIDLFEREE-VGGRLATMMVQ 87

Query: 95  GDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFSLGIWDGHNFVFRTLKSSLTSK 154
           G  +EAG S++HP NLH  RF K L L+  + S      LGI++G   VF        S 
Sbjct: 88  GQEYEAGGSVIHPLNLHMKRFVKDLGLSTVQASGG---LLGIYNGEALVFE------ESN 147

Query: 155 VPFLDKIVELWNQLVMFLRYGFSLLRMEEFVESAVERFSKYYEGFESRPIFETVNEMLNW 214
              ++ I  +W       RYGF  LRM  +VE  +++F + Y        F +V ++L+ 
Sbjct: 148 WFIINVIKLVW-------RYGFQSLRMHMWVEDVLDKFMRIYRYQSHDYAFSSVEKLLHA 207

Query: 215 SGLYNF---TTRTLYEELIDARLSRLLIQELV 244
            G  +F     RTL E L  A  S   + E++
Sbjct: 208 LGGDDFLGMLNRTLLETLQKAGFSEKFLNEMI 217

BLAST of Spg019223 vs. ExPASy Swiss-Prot
Match: Q5R748 (Prenylcysteine oxidase OS=Pongo abelii OX=9601 GN=PCYOX1 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 3.5e-21
Identity = 73/212 (34.43%), Postives = 107/212 (50.47%), Query Frame = 0

Query: 35  ATSQPPPPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDFNIRIFERHNIVGGRMATVNVA 94
           A  + PP  + IIG+GIGG++ +++LRQ         D  I +FER   VGGR+AT+ V 
Sbjct: 28  AELRAPPDKIAIIGAGIGGTSAAYYLRQ-----KFGKDVKIDLFEREE-VGGRLATMMVQ 87

Query: 95  GDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFSLGIWDGHNFVFRTLKSSLTSK 154
           G  +EAG S++HP NL   RF K L L+  + S      LGI++G   VF        S 
Sbjct: 88  GQEYEAGGSVIHPLNLRMKRFVKDLGLSTVQASGG---LLGIYNGETLVFE------ESN 147

Query: 155 VPFLDKIVELWNQLVMFLRYGFSLLRMEEFVESAVERFSKYYEGFESRPIFETVNEMLNW 214
              ++ I  +W       RYGF  LRM  +VE  +++F + Y        F +V ++L+ 
Sbjct: 148 WFIINVIKLVW-------RYGFQSLRMHMWVEDVLDKFMRIYRYQSHDYAFSSVEKLLHA 207

Query: 215 SGLYNF---TTRTLYEELIDARLSRLLIQELV 244
            G  +F     RTL E L  A  S   + E++
Sbjct: 208 LGGDDFLGMLNRTLLETLQKAGFSEKFLNEMI 217

BLAST of Spg019223 vs. ExPASy Swiss-Prot
Match: Q9CQF9 (Prenylcysteine oxidase OS=Mus musculus OX=10090 GN=Pcyox1 PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.1e-19
Identity = 70/213 (32.86%), Postives = 112/213 (52.58%), Query Frame = 0

Query: 35  ATSQPPPPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDFNIRIFERHNIVGGRMATVNVA 94
           A  + PP  + I+G+GIGG++ +++LR+         D  I +FER   VGGR+AT+ V 
Sbjct: 28  AEPRAPPNRIAIVGAGIGGTSSAYYLRK-----KFGKDVKIDVFEREE-VGGRLATLKVQ 87

Query: 95  GDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFSLGIWDGHNFVFRTLKSSLTSK 154
           G  +EAG S++HP NLH  RF K L L+   P+S     +G+++G + VF   +SS    
Sbjct: 88  GHDYEAGGSVIHPLNLHMKRFVKELGLS-SVPASGG--LVGVYNGKSLVFE--ESSW--- 147

Query: 155 VPFLDKIVEL-WNQLVMFLRYGFSLLRMEEFVESAVERFSKYYEGFESRPIFETVNEMLN 214
             F+  +++L W       RYGF  LRM  +VE  +++F + Y        F +V ++++
Sbjct: 148 --FVINVIKLVW-------RYGFQSLRMHMWVEDLLDKFMRIYRYQSHDYAFSSVEKLMH 207

Query: 215 WSG---LYNFTTRTLYEELIDARLSRLLIQELV 244
             G         +TL E L  A  S   + E++
Sbjct: 208 AIGGDDYVRLLNQTLRENLKKAGFSETFLNEMI 217

BLAST of Spg019223 vs. ExPASy TrEMBL
Match: A0A5D3C2L7 (Farnesylcysteine lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001280 PE=3 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 1.6e-112
Identity = 205/231 (88.74%), Postives = 213/231 (92.21%), Query Frame = 0

Query: 14  MYTVPWSMIFTLFLFFLIVPPATSQPPPPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDF 73
           MY V WS+I  LFL FL  PP TS+PPPPTVCIIGSGIGGS+VSHFLRQYAPSYN STDF
Sbjct: 1   MYMVRWSIILPLFLCFLAFPPVTSRPPPPTVCIIGSGIGGSSVSHFLRQYAPSYNVSTDF 60

Query: 74  NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS 133
           NIRIFERHNIVGGRMATVN+AGDTFEAGASILHPKN HALRFT+LLNLTVKKPSSS SFS
Sbjct: 61  NIRIFERHNIVGGRMATVNIAGDTFEAGASILHPKNFHALRFTELLNLTVKKPSSSGSFS 120

Query: 134 LGIWDGHNFVFRTLKSSLTSKVPFLDKIVELWNQLVMFLRYGFSLLRMEEFVESAVERFS 193
           LGIWDGH FVFRTL S LTS VPF+DKIVELWNQLVMFLRYGFSLLRMEEFVE AVE+FS
Sbjct: 121 LGIWDGHKFVFRTLTSGLTSTVPFVDKIVELWNQLVMFLRYGFSLLRMEEFVERAVEQFS 180

Query: 194 KYYEGFESRPIFETVNEMLNWSGLYNFTTRTLYEELIDARLSRLLIQELVT 245
           KYYEGFESRP+FETVNEMLNWSGLY  TTRTLYEELIDARLSRLLIQELVT
Sbjct: 181 KYYEGFESRPVFETVNEMLNWSGLYKLTTRTLYEELIDARLSRLLIQELVT 231

BLAST of Spg019223 vs. ExPASy TrEMBL
Match: A0A5A7T0K1 (Farnesylcysteine lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold103G00050 PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 5.9e-112
Identity = 204/231 (88.31%), Postives = 213/231 (92.21%), Query Frame = 0

Query: 14  MYTVPWSMIFTLFLFFLIVPPATSQPPPPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDF 73
           MY V WS+I  LFL FL  PP TS+PPPPTVCIIGSGIGGS+VSHFLRQYAPSYN STDF
Sbjct: 1   MYMVRWSIIVPLFLCFLAFPPVTSRPPPPTVCIIGSGIGGSSVSHFLRQYAPSYNVSTDF 60

Query: 74  NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS 133
           NIRIFERHNIVGGRMATVN+AGDTFEAGASILHPKN HALRFT+LLNLTVKKPSSS SFS
Sbjct: 61  NIRIFERHNIVGGRMATVNIAGDTFEAGASILHPKNFHALRFTELLNLTVKKPSSSGSFS 120

Query: 134 LGIWDGHNFVFRTLKSSLTSKVPFLDKIVELWNQLVMFLRYGFSLLRMEEFVESAVERFS 193
           LGIWDGH FVFRTL S LTS VPF+DKIVELWNQLVMFLRYGFSLLRMEEFVE AVE+FS
Sbjct: 121 LGIWDGHKFVFRTLTSGLTSTVPFVDKIVELWNQLVMFLRYGFSLLRMEEFVERAVEQFS 180

Query: 194 KYYEGFESRPIFETVNEMLNWSGLYNFTTRTLYEELIDARLSRLLIQELVT 245
           KYYEGFESRP+FETVN+MLNWSGLY  TTRTLYEELIDARLSRLLIQELVT
Sbjct: 181 KYYEGFESRPVFETVNKMLNWSGLYKLTTRTLYEELIDARLSRLLIQELVT 231

BLAST of Spg019223 vs. ExPASy TrEMBL
Match: A0A1S3B5J5 (farnesylcysteine lyase OS=Cucumis melo OX=3656 GN=LOC103486051 PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 5.9e-112
Identity = 204/231 (88.31%), Postives = 213/231 (92.21%), Query Frame = 0

Query: 14  MYTVPWSMIFTLFLFFLIVPPATSQPPPPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDF 73
           MY V WS+I  LFL FL  PP TS+PPPPTVCIIGSGIGGS+VSHFLRQYAPSYN STDF
Sbjct: 16  MYMVRWSIIVPLFLCFLAFPPVTSRPPPPTVCIIGSGIGGSSVSHFLRQYAPSYNVSTDF 75

Query: 74  NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS 133
           NIRIFERHNIVGGRMATVN+AGDTFEAGASILHPKN HALRFT+LLNLTVKKPSSS SFS
Sbjct: 76  NIRIFERHNIVGGRMATVNIAGDTFEAGASILHPKNFHALRFTELLNLTVKKPSSSGSFS 135

Query: 134 LGIWDGHNFVFRTLKSSLTSKVPFLDKIVELWNQLVMFLRYGFSLLRMEEFVESAVERFS 193
           LGIWDGH FVFRTL S LTS VPF+DKIVELWNQLVMFLRYGFSLLRMEEFVE AVE+FS
Sbjct: 136 LGIWDGHKFVFRTLTSGLTSTVPFVDKIVELWNQLVMFLRYGFSLLRMEEFVERAVEQFS 195

Query: 194 KYYEGFESRPIFETVNEMLNWSGLYNFTTRTLYEELIDARLSRLLIQELVT 245
           KYYEGFESRP+FETVN+MLNWSGLY  TTRTLYEELIDARLSRLLIQELVT
Sbjct: 196 KYYEGFESRPVFETVNKMLNWSGLYKLTTRTLYEELIDARLSRLLIQELVT 246

BLAST of Spg019223 vs. ExPASy TrEMBL
Match: A0A6J1EKT3 (farnesylcysteine lyase OS=Cucurbita moschata OX=3662 GN=LOC111435563 PE=3 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 1.0e-111
Identity = 205/231 (88.74%), Postives = 215/231 (93.07%), Query Frame = 0

Query: 14  MYTVPWSMIFTLFLFFLIVPPATSQPPPPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDF 73
           M  V WS+I  LFLFFL VPPA +Q PPPTVCIIGSGIGGS+V+HFLRQYAPSYNAS DF
Sbjct: 1   MMMVRWSIIVPLFLFFLSVPPAIAQQPPPTVCIIGSGIGGSSVAHFLRQYAPSYNASVDF 60

Query: 74  NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS 133
           NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS
Sbjct: 61  NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS 120

Query: 134 LGIWDGHNFVFRTLKSSLTSKVPFLDKIVELWNQLVMFLRYGFSLLRMEEFVESAVERFS 193
           LGIWDG  FVFRTLKS LTSK  +LDKIVELWNQ+VMF+RYGFSLLRME+FVESAVE+FS
Sbjct: 121 LGIWDGRKFVFRTLKSGLTSKFSYLDKIVELWNQVVMFIRYGFSLLRMEKFVESAVEKFS 180

Query: 194 KYYEGFESRPIFETVNEMLNWSGLYNFTTRTLYEELIDARLSRLLIQELVT 245
           KYYEGFESRP FETVN+MLNWSGLYN TTRTLYEELIDARLSRLLIQELVT
Sbjct: 181 KYYEGFESRPAFETVNDMLNWSGLYNLTTRTLYEELIDARLSRLLIQELVT 231

BLAST of Spg019223 vs. ExPASy TrEMBL
Match: A0A6J1HT19 (farnesylcysteine lyase OS=Cucurbita maxima OX=3661 GN=LOC111467195 PE=3 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 5.0e-111
Identity = 202/231 (87.45%), Postives = 215/231 (93.07%), Query Frame = 0

Query: 14  MYTVPWSMIFTLFLFFLIVPPATSQPPPPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDF 73
           M  V WS+I  LFLFFL VPPA +Q PPPTVCIIGSGIGGS+V+HFLRQYAPSYNAS DF
Sbjct: 1   MMMVRWSIIVPLFLFFLSVPPAIAQQPPPTVCIIGSGIGGSSVAHFLRQYAPSYNASVDF 60

Query: 74  NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNLHALRFTKLLNLTVKKPSSSDSFS 133
           NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKN HALRFTKLLNLTVKKPSSSDSFS
Sbjct: 61  NIRIFERHNIVGGRMATVNVAGDTFEAGASILHPKNFHALRFTKLLNLTVKKPSSSDSFS 120

Query: 134 LGIWDGHNFVFRTLKSSLTSKVPFLDKIVELWNQLVMFLRYGFSLLRMEEFVESAVERFS 193
           LGIWDG  FVFRTLKS LTSK P+LDKIVELWNQ+V+F+RYGFSLLRME+FVESAV +FS
Sbjct: 121 LGIWDGRKFVFRTLKSGLTSKFPYLDKIVELWNQVVIFIRYGFSLLRMEKFVESAVGKFS 180

Query: 194 KYYEGFESRPIFETVNEMLNWSGLYNFTTRTLYEELIDARLSRLLIQELVT 245
           KYYEGFESRP+FETVN+MLNWSGLYN TTRTLY+ELIDARLSRLLIQELVT
Sbjct: 181 KYYEGFESRPVFETVNDMLNWSGLYNLTTRTLYDELIDARLSRLLIQELVT 231

BLAST of Spg019223 vs. TAIR 10
Match: AT5G63910.1 (farnesylcysteine lyase )

HSP 1 Score: 231.1 bits (588), Expect = 1.0e-60
Identity = 121/205 (59.02%), Postives = 143/205 (69.76%), Query Frame = 0

Query: 41  PPTVCIIGSGIGGSAVSHFLRQYAPSYNASTDFNIRIFERHNIVGGRMATVNVAGDTFEA 100
           P TVCI+GSGIGGS+V+HFLR Y+ S        I +FERH IVGGRM TV VAGDTFEA
Sbjct: 35  PATVCIVGSGIGGSSVAHFLRNYSVS-TGLNQAKILMFERHEIVGGRMRTVTVAGDTFEA 94

Query: 101 GASILHPKNLHALRFTKLLNLTVKKPSS-SDSFSLGIWDGHNFVFRTLKSSLTSKVPFLD 160
           G SILHPKN H   F +  NLTV+ P+   +S ++GIWDG  FV +T  S   +K PFLD
Sbjct: 95  GGSILHPKNYHVKDFVERFNLTVRLPTPIEESSAIGIWDGKRFVVKTFGSG--TKFPFLD 154

Query: 161 KIVELWNQLVMFLRYGFSLLRMEEFVESAVERFSKYYEGFESRPIFETVNEMLNWSGLYN 220
            IV   N L +FLRYG SLLRM  F+E+ V+ F KYYE  ESRPIF+ V  ML WSGLYN
Sbjct: 155 TIVSWVNDLYLFLRYGLSLLRMSSFIENTVDNFLKYYESLESRPIFDNVEGMLKWSGLYN 214

Query: 221 FTTRTLYEELIDARLSRLLIQELVT 245
            T  TL E+L +A+LS LL+ ELVT
Sbjct: 215 LTKLTLQEKLSEAQLSPLLVNELVT 236

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882019.15.3e-11590.91farnesylcysteine lyase [Benincasa hispida][more]
TYK05432.13.2e-11288.74farnesylcysteine lyase [Cucumis melo var. makuwa][more]
KAG6603171.17.2e-11288.74Farnesylcysteine lyase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033... [more]
XP_023545088.19.3e-11288.31farnesylcysteine lyase [Cucurbita pepo subsp. pepo][more]
KAA0034879.11.2e-11188.31farnesylcysteine lyase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
P576811.5e-5959.02Farnesylcysteine lyase OS=Arabidopsis thaliana OX=3702 GN=FLCY PE=1 SV=1[more]
Q9UHG34.2e-2234.91Prenylcysteine oxidase 1 OS=Homo sapiens OX=9606 GN=PCYOX1 PE=1 SV=3[more]
Q95KC99.3e-2234.43Prenylcysteine oxidase OS=Macaca fascicularis OX=9541 GN=PCYOX1 PE=2 SV=1[more]
Q5R7483.5e-2134.43Prenylcysteine oxidase OS=Pongo abelii OX=9601 GN=PCYOX1 PE=2 SV=1[more]
Q9CQF91.1e-1932.86Prenylcysteine oxidase OS=Mus musculus OX=10090 GN=Pcyox1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3C2L71.6e-11288.74Farnesylcysteine lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold8... [more]
A0A5A7T0K15.9e-11288.31Farnesylcysteine lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1... [more]
A0A1S3B5J55.9e-11288.31farnesylcysteine lyase OS=Cucumis melo OX=3656 GN=LOC103486051 PE=3 SV=1[more]
A0A6J1EKT31.0e-11188.74farnesylcysteine lyase OS=Cucurbita moschata OX=3662 GN=LOC111435563 PE=3 SV=1[more]
A0A6J1HT195.0e-11187.45farnesylcysteine lyase OS=Cucurbita maxima OX=3661 GN=LOC111467195 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G63910.11.0e-6059.02farnesylcysteine lyase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF13450NAD_binding_8coord: 46..113
e-value: 3.0E-12
score: 46.6
NoneNo IPR availablePANTHERPTHR15944:SF0FARNESYLCYSTEINE LYASEcoord: 34..244
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 40..174
e-value: 1.9E-9
score: 39.0
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 40..224
IPR010795Prenylcysteine lyasePFAMPF07156Prenylcys_lyasecoord: 159..244
e-value: 2.8E-17
score: 62.9
IPR017046Prenylcysteine oxidasePANTHERPTHR15944FARNESYLCYSTEINE LYASEcoord: 34..244

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg019223.1Spg019223.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009738 abscisic acid-activated signaling pathway
biological_process GO:0045338 farnesyl diphosphate metabolic process
biological_process GO:0030327 prenylated protein catabolic process
biological_process GO:0030328 prenylcysteine catabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0016829 lyase activity
molecular_function GO:0001735 prenylcysteine oxidase activity
molecular_function GO:0016670 oxidoreductase activity, acting on a sulfur group of donors, oxygen as acceptor