Spg018891 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg018891 vs. NCBI nr
Match: XP_022970204.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima])

HSP 1 Score: 524.2 bits (1349), Expect = 1.2e-144
Identity = 268/486 (55.14%), Postives = 341/486 (70.16%), Query Frame = 0

Query: 16  FILLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLI 75
           F+LLLL     IL+ + S    PPLPPGP PWP+LGNLLQIG N  +++T FS  +GPLI
Sbjct: 8   FLLLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 67

Query: 76  SLRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWK 135
           SL+LG+Q  VVASSP+AAA IL+T DR LS RYIFQ   D       SLL SPEC + WK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 127

Query: 136 FLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTN 195
            LR+ CR  LF+++A++SQA+LRR K   +V +L SK   G  V I+++ F TVFNT++N
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSK--QGSVVEIKELVFITVFNTLSN 187

Query: 196 VIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRRV 255
            IFS+DL+ + GDG  G K  ++  ++LGL+PNLADFYP+L   DLQGLRKK+A    +V
Sbjct: 188 FIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQV 247

Query: 256 DSVYDTFIAERRRDRDRNGGAAT--------GPFGN--------EILSASTDTTSTTVEW 315
           DS ++  I ERR    R+  AA+          F +        E+LSA TDTT+TTVEW
Sbjct: 248 DSSWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTVEW 307

Query: 316 TMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFLLP 375
            MAEL+KN+ IM++V EE  + I     AIDES + +LHYL  CVKETLRLHPPAPFLLP
Sbjct: 308 AMAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLP 367

Query: 376 HRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDFRYL 435
             A + C++MGY++PKDT IFVN+W IGRDP+ WE+P +F P+RF G D + KG D+R+L
Sbjct: 368 RYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDYRFL 427

Query: 436 PFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKPLS 486
           PFG GRRICPGLPM  VQV LILATLI  F+WYLPN+ED S LDL+G++G+   KE+PL 
Sbjct: 428 PFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEPLK 487

BLAST of Spg018891 vs. NCBI nr
Match: KAG7017133.1 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 519.6 bits (1337), Expect = 2.9e-143
Identity = 267/487 (54.83%), Postives = 342/487 (70.23%), Query Frame = 0

Query: 16  FILLLLLPFLL-ILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPL 75
           F+LLLLLP ++ ILK + S    PPLPPGP PWP+LGNLLQIG N  +++T FS ++GPL
Sbjct: 8   FLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPL 67

Query: 76  ISLRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHW 135
           ISL+LG+Q  VVASSP+AAA IL+T DR LS RYIFQ + D       SL+ SPEC + W
Sbjct: 68  ISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRW 127

Query: 136 KFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMT 195
           K LR+ CR  LF+++A++SQA+LRR K   +V +L SK   G  V I++  F TVFNT++
Sbjct: 128 KNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSK--QGSVVEIKEFVFITVFNTLS 187

Query: 196 NVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRR 255
           N IFS+DL+ + GDG  G K H++  ++L L+PNLADFYP+L   DLQGLRKK+A    +
Sbjct: 188 NFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAAIYKNQ 247

Query: 256 VDSVYDTFIAERRRDRDRNGGAAT--------GPFGN--------EILSASTDTTSTTVE 315
           +DS ++  I ERR    R   AAT          + N        E+LSA TDT  TTVE
Sbjct: 248 IDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDT--TTVE 307

Query: 316 WTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFLL 375
           W MAEL+KN+ IM++V EE  + I     AIDES + +LHYL  CVKETLRLHPPAPFLL
Sbjct: 308 WAMAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPPAPFLL 367

Query: 376 PHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDFRY 435
           P  A + C++MGY++PK T IFVN+W IGRDP+ WE+P +F P+RF G D + KG D+R+
Sbjct: 368 PRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRF 427

Query: 436 LPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKPL 486
           LPFG GRR+CPGLPM  VQV LILATLI  F+W LPN+E+ S LDL+G++GI   KE+PL
Sbjct: 428 LPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEPL 487

BLAST of Spg018891 vs. NCBI nr
Match: XP_023551257.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 519.6 bits (1337), Expect = 2.9e-143
Identity = 266/490 (54.29%), Postives = 340/490 (69.39%), Query Frame = 0

Query: 13  MAPFILLLLLPFLL-ILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLH 72
           +A F  LLLLP +  +LK + S    PPLPPGP PWP+LGNLLQIG N  +++T FS ++
Sbjct: 3   IASFSFLLLLPLIFTVLKHISSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHIY 62

Query: 73  GPLISLRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECD 132
           GPLISL+LG+Q  VVASSP+AAA IL+T DR LS RYI Q   D       SLL SPEC 
Sbjct: 63  GPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYILQMTPDRALHDNCSLLFSPECG 122

Query: 133 EHWKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFN 192
           + WK LR+ CR  LF+++A++SQA+LRR K   +V +L SK   G  V I++  F TVFN
Sbjct: 123 DRWKNLRSICRAKLFTARAIESQASLRRKKMKDMVEFLQSK--QGSVVEIKEFVFITVFN 182

Query: 193 TMTNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAEC 252
           T++N+IFS+DL+++ GDG  G K  ++  ++LGL+PNLADFYP+L   DLQGLRKK+A  
Sbjct: 183 TLSNLIFSRDLVDFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRSFDLQGLRKKAAIY 242

Query: 253 SRRVDSVYDTFIAERRRDRDRNGGAAT--------GPFGN--------EILSASTDTTST 312
             ++DS  +  I ERR    R   AA+          F N        E+L A TDTT+T
Sbjct: 243 KNQIDSSSEILIKERRTIHSRGVSAASDFLDILIESGFTNEQISYLILEVLLAGTDTTTT 302

Query: 313 TVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAP 372
           TVEW MAELMKN+ IM++V EE  + +     AIDES + +LHYL  CVKET+RLHPPAP
Sbjct: 303 TVEWAMAELMKNRDIMNRVREETKKAL--NGGAIDESKLSELHYLWKCVKETVRLHPPAP 362

Query: 373 FLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFD 432
           FLLP  A + C++MGY++PK T IFVN+W IGRDP+ WE+P +F P+RF  SD + KG D
Sbjct: 363 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDSSDADLKGHD 422

Query: 433 FRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKE 486
           +R+LPFG GRR CPGLPM  VQV LILATLI  F+W LPN+ED S LDL+G++GI   KE
Sbjct: 423 YRFLPFGGGRRTCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGITLQKE 482

BLAST of Spg018891 vs. NCBI nr
Match: KAE8647950.1 (hypothetical protein Csa_000545 [Cucumis sativus])

HSP 1 Score: 519.6 bits (1337), Expect = 2.9e-143
Identity = 264/494 (53.44%), Postives = 347/494 (70.24%), Query Frame = 0

Query: 13  MAPFILLLLLPFLLILKLLKSRK-HSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLH 72
           + P +LLL+ P  +ILK +KS      PLPPGP PWP+LGNL QIG N  ++IT F+ ++
Sbjct: 9   LLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIY 68

Query: 73  GPLISLRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECD 132
           GPLISL+LG+Q  VVASSP+ A  +L+THDR LS RYI Q   D     + SL+ SPEC 
Sbjct: 69  GPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECG 128

Query: 133 EHWKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFN 192
           +HWK LR+ C+ +LF++KA++SQA LR  K   LV +L SK   G  V I+D  FT+VFN
Sbjct: 129 DHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESK--QGSVVKIKDFVFTSVFN 188

Query: 193 TMTNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAEC 252
           T++N+IFS DL+++ GDG  G K  ++K ++LGL PNLADFYP+L   DLQGLRKK+A  
Sbjct: 189 TLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIY 248

Query: 253 SRRVDSVYDTFIAERRRDRDRNGGAATGPFG----------------NEILSASTDTTST 312
              VDS++   I ERR    + G A++                    NE+L A+TDTT+T
Sbjct: 249 MNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLFAATDTTTT 308

Query: 313 TVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAP 372
           TVEW M+E++K+K I++K  EEI +++  ++ AIDES I +L YL +CVKETLRLHPP P
Sbjct: 309 TVEWAMSEILKDKDILNKAREEI-KKVAKESPAIDESNISKLQYLWSCVKETLRLHPPVP 368

Query: 373 FLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRF---LGSDIEFK 432
           FLLP  A   C+VMGY+IPKD+ IFVN+W IGRDP+ WE+  TFNP+RF    G++++FK
Sbjct: 369 FLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFK 428

Query: 433 GFDFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVC 487
           G+D+RYLPFG GRRICPGLPM IVQV LILATL+  F+W LPN ED SKLDL+G++G+  
Sbjct: 429 GYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTL 488

BLAST of Spg018891 vs. NCBI nr
Match: XP_031741123.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 519.6 bits (1337), Expect = 2.9e-143
Identity = 265/491 (53.97%), Postives = 345/491 (70.26%), Query Frame = 0

Query: 13  MAPFILLLLLPFLLILKLLKSRK-HSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLH 72
           + P +LLL+ P  +ILK +KS      PLPPGP PWP+LGNL QIG N  ++IT F+ ++
Sbjct: 9   LLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIY 68

Query: 73  GPLISLRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECD 132
           GPLISL+LG+Q  VVASSP+ A  +L+THDR LS RYI Q   D     + SL+ SPEC 
Sbjct: 69  GPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECG 128

Query: 133 EHWKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFN 192
           +HWK LR+ C+ +LF++KA++SQA LR  K   LV +L SK   G  V I+D  FT+VFN
Sbjct: 129 DHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESK--QGSVVKIKDFVFTSVFN 188

Query: 193 TMTNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAEC 252
           T++N+IFS DL+++ GDG  G K  ++K ++LGL PNLADFYP+L   DLQGLRKK+A  
Sbjct: 189 TLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIY 248

Query: 253 SRRVDSVYDTFIAERRRDRDRNGGAATGPFG----------------NEILSASTDTTST 312
              VDS++   I ERR    + G A++                    NE+LSA TDTT+T
Sbjct: 249 MNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAGTDTTTT 308

Query: 313 TVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAP 372
           TVEW M+E++K+K I++KV +EI +  K+  + IDES I +L YL +CVKETLRLHPPAP
Sbjct: 309 TVEWAMSEILKDKGILNKVRQEIKKVTKE--SPIDESNISKLQYLWSCVKETLRLHPPAP 368

Query: 373 FLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRF---LGSDIEFK 432
           FLLP  A   C+VMGY+IPKD+ IFVN+W IGRDP+ WE+  TFNP+RF    G++++FK
Sbjct: 369 FLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFK 428

Query: 433 GFDFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVC 484
           G+D+RYLPFG GRRICPGLPM IVQV LILATL+  F+W LPN ED SKLDL+G++G+  
Sbjct: 429 GYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTL 488

BLAST of Spg018891 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 4.5e-107
Identity = 209/491 (42.57%), Postives = 295/491 (60.08%), Query Frame = 0

Query: 17  ILLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLIS 76
           I ++   FLL L  +        LPPGP P P++GNLLQ+G  P       +  +G L S
Sbjct: 6   IAIVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGELFS 65

Query: 77  LRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWKF 136
           L+LGSQ  VVASSP+AAAEIL+THD+ LSGRY+FQ+      +   + ++  EC+++WK 
Sbjct: 66  LKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVE--NSIVWSECNDNWKL 125

Query: 137 LRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTNV 196
           LR  CRT LF+ K ++SQ+ +R  KA  +V +L  K   GE V I +V F T+ N   N+
Sbjct: 126 LRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGK--EGEVVKIVEVVFGTLVNIFGNL 185

Query: 197 IFSKDLMEYEG--DGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRR 256
           IFSKD+ + E    G+   KEH  K L++G   N AD++PI+  LDL G R+  AE  ++
Sbjct: 186 IFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQ 245

Query: 257 VDSVYDTFIAERRRDRDRNGGAATGPFGN-----------------EILSASTDTTSTTV 316
           +  V+   + ERR  +   G  +   F +                 E+  A T+T+++T+
Sbjct: 246 IYDVWGVMLKERRGTK---GSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTI 305

Query: 317 EWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFL 376
           EW + EL K   ++ K+  E+   + D    + ES +P L YLQA VKETLRLHPP P L
Sbjct: 306 EWAITELTKKPLVVSKIRLELVNVVGDN--TVKESDLPHLPYLQAFVKETLRLHPPTPLL 365

Query: 377 LPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDFR 436
           LP RA++TC VM YTIPK+ +I VN WAIGRDP  W++P  F P+RFL SD+++KG DF 
Sbjct: 366 LPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFE 425

Query: 437 YLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKP 489
            +PFG GRRICPGLP+     NLI+ATL++ F+W LP     S+L +  K G+   K+ P
Sbjct: 426 LIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPP 485

BLAST of Spg018891 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.0e-106
Identity = 203/471 (43.10%), Postives = 285/471 (60.51%), Query Frame = 0

Query: 34  RKHSPP-LPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLISLRLGSQLAVVASSPSA 93
           R  SP  LPPGP PWP++GNLLQ+G  P       +  +G L SL+LGS+  VVAS+P A
Sbjct: 16  RDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLA 75

Query: 94  AAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWKFLRTTCRTSLFSSKALD 153
           A+EIL+THDR LSGRY+FQ+      +   + ++  EC+E WK LR  CRT LF+ K ++
Sbjct: 76  ASEILKTHDRVLSGRYVFQSFRVKEHVE--NSIVWSECNETWKKLRKVCRTELFTQKMIE 135

Query: 154 SQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTNVIFSKDLMEY--EGDGA 213
           SQA +R +KA+ +V YL  K   G  V I +V F T+ N   N+IFS+++ +   E  G+
Sbjct: 136 SQAEVRESKAMEMVEYL--KKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGS 195

Query: 214 YGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRRVDSVYDTFIAERRRDR 273
              KEH  + LELG   N AD++P L   DL G  K  A+C + + SV+   + ER+  +
Sbjct: 196 VEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAK 255

Query: 274 DRNGGAAT----------------GPFGNEILSASTDTTSTTVEWTMAELMKNKHIMHKV 333
             N                          EI  A T+T+++T+EW ++EL KN  +   +
Sbjct: 256 QHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANM 315

Query: 334 HEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFLLPHRAVQTCQVMGYTIP 393
             E+   +  +   + ES IP + YLQA VKETLRLHP  P LLP RA++TC+V+ YTIP
Sbjct: 316 RLELLSVVGKR--PVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIP 375

Query: 394 KDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDFRYLPFGSGRRICPGLPMG 453
           K+ +I VN W IGRDP  W +P  F+P+RFL S I+FKG DF  +PFG+GRRICPG+P+ 
Sbjct: 376 KECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLA 435

Query: 454 IVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKPLSLIPKIR 486
              ++LI+++L++ FDW  P   DPS+L +  K G+   KE PL ++PK R
Sbjct: 436 TQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKTR 480

BLAST of Spg018891 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 4.7e-104
Identity = 202/489 (41.31%), Postives = 291/489 (59.51%), Query Frame = 0

Query: 17  ILLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLIS 76
           + L+ +    IL LL        LPPGP PWP++GNLLQ+G  P       +  +G + +
Sbjct: 5   VALIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFT 64

Query: 77  LRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWKF 136
           L++G++  VVAS+ SAA+EIL+THDR LS RY+FQ+      +   + ++  +C E WK 
Sbjct: 65  LKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVE--NSIVWSDCTETWKN 124

Query: 137 LRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTNV 196
           LR  CRT LF+ K ++SQA +R  K   +V YL  K   GE V I +V F T+ N   N+
Sbjct: 125 LRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKK--QGEEVKIVEVIFGTLVNIFGNL 184

Query: 197 IFSKDLMEY--EGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRR 256
           IFS+++ E      G+  FKE+  + LELG   N AD++P+L   DL G RK+ AEC + 
Sbjct: 185 IFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKG 244

Query: 257 VDSVYDTFIAERRRDRDRNGGAATGPFGN-----------------EILSASTDTTSTTV 316
           + +++   + ER+  +  +G  +   F +                 E+  A T+T+++T+
Sbjct: 245 IYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTI 304

Query: 317 EWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFL 376
           EW M EL KN  I  K+  EI   + ++  ++ ES  P L YL+A VKETLRLHPP P L
Sbjct: 305 EWAMTELTKNPKITAKIRSEIQTVVGER--SVKESDFPNLPYLEATVKETLRLHPPTPLL 364

Query: 377 LPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDFR 436
           LP RA++TC ++ YTIPKD +I VN W IGRDP  W +P TF+P+RFL S ++F+G DF 
Sbjct: 365 LPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFS 424

Query: 437 YLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKP 487
            +PFG+GRRICPGLP+    + L++AT ++  DW LPN      L +  K G+   KE P
Sbjct: 425 LIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPP 484

BLAST of Spg018891 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.8e-103
Identity = 201/489 (41.10%), Postives = 291/489 (59.51%), Query Frame = 0

Query: 17  ILLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLIS 76
           + L+ +    IL LL        LPPGP PWP++GNLLQ+G  P       +  +G + +
Sbjct: 6   VALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFT 65

Query: 77  LRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWKF 136
           L++G++  VVAS+ SAA+EIL+THDR LS RY+FQ+      +   + ++  +C E WK 
Sbjct: 66  LKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVE--NSIVWSDCTETWKN 125

Query: 137 LRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTNV 196
           LR  CRT LF+ K ++SQA +R  K   +V YL  K   GE V I +V F T+ N   N+
Sbjct: 126 LRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKK--QGEEVKIVEVIFGTLVNIFGNL 185

Query: 197 IFSKDLME--YEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRR 256
           IFS+++ E      G+  FKE+  + LELG   N AD++P+L   DL G RK+ AEC + 
Sbjct: 186 IFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKG 245

Query: 257 VDSVYDTFIAERRRDRDRNGGAATGPFGN-----------------EILSASTDTTSTTV 316
           + +++   + ER+  +  +G  +   F +                 E+  A T+T+++T+
Sbjct: 246 IYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTI 305

Query: 317 EWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFL 376
           EW M EL KN  I  K+  E+   + ++  ++ ES  P L YL+A VKETLRLHPP P L
Sbjct: 306 EWAMTELTKNPKITAKLRSELQTVVGER--SVKESDFPNLPYLEATVKETLRLHPPTPLL 365

Query: 377 LPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDFR 436
           LP RA++TC ++ YTIPKD +I VN W IGRDP  W +P TF+P+RFL S ++F+G DF 
Sbjct: 366 LPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFS 425

Query: 437 YLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKP 487
            +PFG+GRRICPGLP+    + L++AT ++  DW LPN      L +  K G+   KE P
Sbjct: 426 LIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPP 485

BLAST of Spg018891 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 2.1e-96
Identity = 197/486 (40.53%), Postives = 293/486 (60.29%), Query Frame = 0

Query: 18  LLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPN---PL-LAITHFSTLHGP 77
           L  L+P +L+  LL   K+   LPPGP PWPL+GNL  +  N   PL + + + +  HGP
Sbjct: 7   LFSLIPVILVFILLLKPKYK-NLPPGPHPWPLIGNLPILFTNTEVPLHITLANMARTHGP 66

Query: 78  LISLRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECDEH 137
           ++ L LG+Q  V+AS+  AA EIL+THDR  S R+I  +    H + ++SL+ S +C ++
Sbjct: 67  IMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHI-KYSLVWS-DCTDY 126

Query: 138 WKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTM 197
           WK LR   RT +FS K L +Q+ +R  K   L+ +L SK   G+ V I    F T+ N +
Sbjct: 127 WKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSK--EGQVVKISQFVFGTLLNIL 186

Query: 198 TNVIFSKDLMEY-EGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECS 257
            NV+FSKD+  Y +     G +   R+ L +G  PN+A+FYP L  LDLQGL+KK  E  
Sbjct: 187 GNVVFSKDVFVYGDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERF 246

Query: 258 RRVDSVYDTFIAERRRDRDRN-------------GGAATGPFGNEILSASTDTTSTTVEW 317
            RV  +++  + ER+ +R+                 A       E     ++T+S T+EW
Sbjct: 247 IRVMKMWEGTVKERKANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEW 306

Query: 318 TMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFLLP 377
            +AEL+K+   M KV +E+   +    + I ES +PQL YLQAC+KE +RLHP APFLLP
Sbjct: 307 VIAELIKSPKEMAKVRKELNEVV--GTSTIKESDLPQLPYLQACIKEAMRLHPAAPFLLP 366

Query: 378 HRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDFRYL 437
            RA +TC+VMGYTIPK++++ VN +AIGRDP +W++PSTF P+RFL SD++F G  ++++
Sbjct: 367 RRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDFHGAHYQFI 426

Query: 438 PFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKPLS 486
           PFGSGRR C G+P+    + LI+ +L+  +D+ LP    P  L ++  + +    +  L 
Sbjct: 427 PFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLAIDPSLC 485

BLAST of Spg018891 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 5.7e-145
Identity = 268/486 (55.14%), Postives = 341/486 (70.16%), Query Frame = 0

Query: 16  FILLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLI 75
           F+LLLL     IL+ + S    PPLPPGP PWP+LGNLLQIG N  +++T FS  +GPLI
Sbjct: 8   FLLLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 67

Query: 76  SLRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWK 135
           SL+LG+Q  VVASSP+AAA IL+T DR LS RYIFQ   D       SLL SPEC + WK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 127

Query: 136 FLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTN 195
            LR+ CR  LF+++A++SQA+LRR K   +V +L SK   G  V I+++ F TVFNT++N
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSK--QGSVVEIKELVFITVFNTLSN 187

Query: 196 VIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRRV 255
            IFS+DL+ + GDG  G K  ++  ++LGL+PNLADFYP+L   DLQGLRKK+A    +V
Sbjct: 188 FIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQV 247

Query: 256 DSVYDTFIAERRRDRDRNGGAAT--------GPFGN--------EILSASTDTTSTTVEW 315
           DS ++  I ERR    R+  AA+          F +        E+LSA TDTT+TTVEW
Sbjct: 248 DSSWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTVEW 307

Query: 316 TMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFLLP 375
            MAEL+KN+ IM++V EE  + I     AIDES + +LHYL  CVKETLRLHPPAPFLLP
Sbjct: 308 AMAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLP 367

Query: 376 HRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDFRYL 435
             A + C++MGY++PKDT IFVN+W IGRDP+ WE+P +F P+RF G D + KG D+R+L
Sbjct: 368 RYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDYRFL 427

Query: 436 PFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKPLS 486
           PFG GRRICPGLPM  VQV LILATLI  F+WYLPN+ED S LDL+G++G+   KE+PL 
Sbjct: 428 PFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEPLK 487

BLAST of Spg018891 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 4.1e-143
Identity = 265/486 (54.53%), Postives = 340/486 (69.96%), Query Frame = 0

Query: 16  FILLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLI 75
           F+LLLL    +IL+ + S    PPLPPGP PWP+LGNLLQIG N  +++T FS ++GPLI
Sbjct: 8   FLLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 67

Query: 76  SLRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWK 135
           SL+LG+Q  VVASSP+AAA IL+T DR LS RYIFQ   D       SLL SPEC + WK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 127

Query: 136 FLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTN 195
            LR+ CR  LF+++A++SQA+LRR K   +V +L SK   G  V I+++ F TVFNT++N
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSK--QGSVVEIKELVFITVFNTLSN 187

Query: 196 VIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRRV 255
            IFS+DL+ + GDG  G K  ++  ++LGL+PNLADFYP+L   DLQGLRKK+A    +V
Sbjct: 188 FIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQV 247

Query: 256 DSVYDTFIAERRRDRDRNGGAAT--------GPFGN--------EILSASTDTTSTTVEW 315
           DS ++  I ERR    +   AA+          F +        E+LSA TDTT+TTVEW
Sbjct: 248 DSSWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEW 307

Query: 316 TMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFLLP 375
            MAEL+KN+ IM++V EE  + I     AIDES + +LHYL  CVKETLRLHPPAPFLLP
Sbjct: 308 AMAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLP 367

Query: 376 HRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDFRYL 435
             A + C++MGY++PK T IFVN+W IGRDP+ WE+P +F P+RF G D + KG D+R+L
Sbjct: 368 RYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFL 427

Query: 436 PFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKPLS 486
           PFG GRRICPGLPM  VQV LILATLI  F+W LPN+ED S LDL+G++G+   KE+PL 
Sbjct: 428 PFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLK 487

BLAST of Spg018891 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 1.2e-142
Identity = 265/489 (54.19%), Postives = 344/489 (70.35%), Query Frame = 0

Query: 18  LLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLISL 77
           LLLLLP  +ILK +KS     PLPPGP PWP+LGNLLQIG N  ++IT F+ ++GPLISL
Sbjct: 13  LLLLLPIFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLISL 72

Query: 78  RLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWKFL 137
           +LG+Q  +VASSP+AA  +L+T DR LS RYIFQ   D     + SL+ SPEC + WK L
Sbjct: 73  KLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQWKNL 132

Query: 138 RTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTNVI 197
           R+ C+ +LF++KA++SQA LR  K   LV +L SK   G  V ++D  FT+VFNT++N+I
Sbjct: 133 RSLCKVNLFTAKAIESQAILREKKMKELVKFLESK--QGSVVEVKDFVFTSVFNTLSNLI 192

Query: 198 FSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRRVDS 257
           FS DL+++ GDG  G K  ++K ++LGL PNLADFYP+L   DLQGL+ K+      VDS
Sbjct: 193 FSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVDS 252

Query: 258 VYDTFIAERRRDRDRNGGAAT--------GPFGN--------EILSASTDTTSTTVEWTM 317
           ++   I ERR    + G A+           F +        E+LSA TDTT+TTVEW M
Sbjct: 253 LWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEWAM 312

Query: 318 AELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFLLPHR 377
           +E++K+K I++KV EE+ +++  +N  IDES I +L YL  CVKETLRLHPPAPFLLP  
Sbjct: 313 SEILKDKDILNKVREEM-KKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPRL 372

Query: 378 AVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRF---LGSD-IEFKGFDFR 437
           A   C+VMGY+IPKD+ +FVN+W IGRDP+ WE+  TFNP RF    G+D ++FKG+D+R
Sbjct: 373 APMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDYR 432

Query: 438 YLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKP 487
           YLPFG GRRICPGLPM IVQV LILATLI  F+W LPN+ED SKLDL+G++G+   K + 
Sbjct: 433 YLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYET 492

BLAST of Spg018891 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 1.5e-142
Identity = 265/489 (54.19%), Postives = 344/489 (70.35%), Query Frame = 0

Query: 18  LLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLISL 77
           LLLLLP  +ILK +KS     PLPPGP PWP+LGNLLQIG N  ++IT F+ ++GPLISL
Sbjct: 13  LLLLLPIFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLISL 72

Query: 78  RLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWKFL 137
           +LG+Q  +VASSP+AA  +L+T DR LS RYIFQ   D     + SL+ SPEC + WK L
Sbjct: 73  KLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQWKNL 132

Query: 138 RTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTNVI 197
           R+ C+ +LF++KA++SQA LR  K   LV +L SK   G  V ++D  FT+VFNT++N+I
Sbjct: 133 RSLCKVNLFTAKAIESQAILREKKMKELVKFLESK--QGSVVEVKDFVFTSVFNTLSNLI 192

Query: 198 FSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRRVDS 257
           FS DL+++ GDG  G K  ++K ++LGL PNLADFYP+L   DLQGL+ K+      VDS
Sbjct: 193 FSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVDS 252

Query: 258 VYDTFIAERRRDRDRNGGAAT--------GPFGN--------EILSASTDTTSTTVEWTM 317
           ++   I ERR    + G A+           F +        E+LSA TDTT+TTVEW M
Sbjct: 253 LWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEWAM 312

Query: 318 AELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFLLPHR 377
           +E++K+K I++KV EE+ +++  +N  IDES I +L YL  CVKETLRLHPPAPFLLP  
Sbjct: 313 SEILKDKDILNKVREEM-KKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPRL 372

Query: 378 AVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRF---LGSD-IEFKGFDFR 437
           A   C+VMGY+IPKD+ +FVN+W IGRDP+ WE+  TFNP RF    G+D ++FKG+D+R
Sbjct: 373 APMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDYR 432

Query: 438 YLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKP 487
           YLPFG GRRICPGLPM IVQV LILATLI  F+W LPN+ED SKLDL+G++G+   K + 
Sbjct: 433 YLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYET 492

BLAST of Spg018891 vs. ExPASy TrEMBL
Match: A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 2.9e-141
Identity = 262/492 (53.25%), Postives = 338/492 (68.70%), Query Frame = 0

Query: 13  MAPFILLLLLPFLL-ILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLH 72
           +A F  LLLLP +  ILK + S    PP PPGP PWP+LGNLLQIG N  +++T FS ++
Sbjct: 3   IASFSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIY 62

Query: 73  GPLISLRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECD 132
           GPLISL+LG+Q  VVASSP+AAA IL+T DR LS RYIFQ + D       SL+ SPEC 
Sbjct: 63  GPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECG 122

Query: 133 EHWKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFN 192
           + WK LR+ CR  LF+++A++SQA+LRR K   +V +L SK   G  V I++  F TVFN
Sbjct: 123 DRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSK--QGSVVEIKEFVFITVFN 182

Query: 193 TMTNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAEC 252
           T++N IFS+DL+ + GDG  G + H++  ++  L+PNLADFYP+L   DLQGLRKK+A  
Sbjct: 183 TLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIY 242

Query: 253 SRRVDSVYDTFIAERRRDRDRNGGAATGPFGN-----------------EILSASTDTTS 312
             ++DS ++  I ERR    R G   T  F +                 E+LSA TDTT+
Sbjct: 243 KNQIDSSWEILIKERRTIHSR-GVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTT 302

Query: 313 TTVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPA 372
           TTVEW MAEL+KN+ IM++V EE  + I     AIDES + +LHYL  CVKETLRLHPPA
Sbjct: 303 TTVEWAMAELLKNRDIMNRVREETKKAINGD--AIDESKLSELHYLWKCVKETLRLHPPA 362

Query: 373 PFLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGF 432
           PFLLP  A + C++MGY++PK T I VN+W IGRDP+ WE+P +F P+RF   D + KG 
Sbjct: 363 PFLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGH 422

Query: 433 DFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYK 487
           D+R+LPFG GRR+CPGLPM  VQV LILATLI  F+W LPN+ED S LDL G++G+   K
Sbjct: 423 DYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQK 482

BLAST of Spg018891 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 339.0 bits (868), Expect = 6.5e-93
Identity = 197/499 (39.48%), Postives = 274/499 (54.91%), Query Frame = 0

Query: 16  FILLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLI 75
           F+L   + F    +   SRK   P PPGP   P++GN+  +G NP  +    S  +GP++
Sbjct: 15  FVLSFFIIFFTTTRPRSSRK-VVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGPIM 74

Query: 76  SLRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLR-----RFSLLLSPEC 135
           SL+ GS   VV +SP AA E+LRT+D+ LS R      T T+ +R     + S++  P  
Sbjct: 75  SLKFGSLNTVVVTSPEAAREVLRTYDQILSSR------TPTNSIRSINHDKVSVVWLPPS 134

Query: 136 DEHWKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVF 195
              W+ LR    T LFS + +++   LR NK   LV +++  +E  E V I    F T  
Sbjct: 135 SSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFITAL 194

Query: 196 NTMTNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAE 255
           N ++N++FS DL  Y+ + +  F++     +E    P+ A+F+P L  LDLQG RK    
Sbjct: 195 NIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLKA 254

Query: 256 CSRRVDSVYDTFI----AERR---------RDRDRNGGAATGPFGNE------------- 315
           CS R+  V+  FI    AE+          R+RD          G+E             
Sbjct: 255 CSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAELNTNDIVHLLL 314

Query: 316 -ILSASTDTTSTTVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQAC 375
            +  A TDT S+TVEW MAEL++N   M K   EI   I  Q   ++ES I  L YLQA 
Sbjct: 315 DLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVI-GQKGVVEESDISALPYLQAV 374

Query: 376 VKETLRLHPPAPFLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDR 435
           VKET RLHP AP L+P +A    +V+G+ +PKDT++FVN+WAIGRDP  WEN S F P+R
Sbjct: 375 VKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPER 434

Query: 436 FLGSDIEFKGFDFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLD 483
           FLG DI+ +G D+   PFG+GRRICPGLP+ +  V L+LA+L+  FDW LPN      LD
Sbjct: 435 FLGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDLD 494

BLAST of Spg018891 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 332.0 bits (850), Expect = 7.9e-91
Identity = 193/503 (38.37%), Postives = 280/503 (55.67%), Query Frame = 0

Query: 10  VKAMAPFILLLLL-PFLLILKLLKSRKHS---PPLPPGPTPWPLLGNLLQIGPNPLLAIT 69
           +   A F+L   +    LI    +SR+ S     LPPGP   P++GN+ Q+G NP  +  
Sbjct: 4   ISGQALFLLFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFA 63

Query: 70  HFSTLHGPLISLRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLL 129
             + ++GP++SL+ G   +VV +SP AA E+LRTHD+ LSGR    ++         S++
Sbjct: 64  DLAKIYGPIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGRKSNDSIR-CFGHEEVSVI 123

Query: 130 LSPECDEHWKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVA 189
             P     W+ LR    T +FS +  ++  ALR  K   LV ++   +E  E V I   +
Sbjct: 124 WLPPSSARWRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRAS 183

Query: 190 FTTVFNTMTNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLR 249
           +TTV N ++N++FS DL  Y+   +  F++     +E    P+ A+++P +  LDLQG R
Sbjct: 184 YTTVLNIISNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNR 243

Query: 250 KKSAECSRRVDSVY----DTFIAERR--------RDRD-------RNGGAATGPFGNEI- 309
           K     + R+  V+    D  IAE+          +RD        N G       N+I 
Sbjct: 244 KAMRGLTERLFRVFRGFMDAKIAEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIE 303

Query: 310 ------LSASTDTTSTTVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHY 369
                  +A TDT+S+T+EW MAEL++N   M K   E+ R +  QN+ + ES I  L Y
Sbjct: 304 HLLLDMFTAGTDTSSSTLEWAMAELLRNPKTMVKAQAEMDR-VLGQNSVVQESDISGLPY 363

Query: 370 LQACVKETLRLHPPAPFLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTF 429
           LQA VKET RLHP AP L+P +A    +V+G+ +PKDT++ VN+WAIGRDP+ WENPS F
Sbjct: 364 LQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQF 423

Query: 430 NPDRFLGSDIEFKGFDFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDP 483
            P+RF+G DI+ KG D+   PFG GRRICPGLP+ +  V+L+LA+L+  FDW LPN    
Sbjct: 424 EPERFMGKDIDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVS 483

BLAST of Spg018891 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 328.2 bits (840), Expect = 1.1e-89
Identity = 193/495 (38.99%), Postives = 274/495 (55.35%), Query Frame = 0

Query: 17  ILLLLLPFLLILKLLKSRKHSPPL---PPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGP 76
           I   +L  LL     +SR+    L   PPGP   P++GN+  +G NP  + T  S  +GP
Sbjct: 12  IFCFILSCLLFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHHSFTDLSKTYGP 71

Query: 77  LISLRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPECDEH 136
           ++SL+LG   +VV +S  A  E+L+THD+ LSGRYI +  T ++    FS+         
Sbjct: 72  VMSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISE-ATKSNNHHEFSVGWIHPSSSR 131

Query: 137 WKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTM 196
           ++ LR    T LFS + + +  ALR  K   LV++L+   E  E V I  V+F T  N +
Sbjct: 132 FRMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDISHVSFVTALNII 191

Query: 197 TNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSR 256
           +N++FS +L  Y+   +  F+E      E    P+LA+F+P +  LDLQG  KK  E S 
Sbjct: 192 SNILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQGNSKKMRESSG 251

Query: 257 RVDSVYDTFIAER---RRDRDRNGGAATGPF---------GNE--------------ILS 316
           R+  V+  F   R   +  R      ++  F         G+E              +  
Sbjct: 252 RLLQVFREFYDARIVEKSSRSVEKDVSSKDFLDVLIDLQQGDETEINIDEIEHLLLDMFV 311

Query: 317 ASTDTTSTTVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKET 376
           A TDT S+TVEW MAEL+ N   M KV +EI   I  QN    ES I +L YL+A VKET
Sbjct: 312 AGTDTNSSTVEWAMAELLGNPKTMTKVQDEINHVI-GQNGDFQESDISKLPYLKAVVKET 371

Query: 377 LRLHPPAPFLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGS 436
            RLHP APFLL  +A    +++G+T+ KD+++ VN+WAIGRDP  WENP+ F P+RFLG 
Sbjct: 372 FRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVWENPTHFEPERFLGK 431

Query: 437 DIEFKGFDFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGK 483
           +I+ KG D+   PFG+GRRICPGLP+ +  V+L+LA+L+  F+W LPN      LD+   
Sbjct: 432 EIDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKLPNGVGSEDLDMEET 491

BLAST of Spg018891 vs. TAIR 10
Match: AT2G45580.1 (cytochrome P450, family 76, subfamily C, polypeptide 3 )

HSP 1 Score: 326.2 bits (835), Expect = 4.4e-89
Identity = 189/514 (36.77%), Postives = 280/514 (54.47%), Query Frame = 0

Query: 7   VSRVKAMAPFILLLLLPFLLILKLLKSRKHSP--PLPPGPTPWPLLGNLLQIGPNPLLAI 66
           +S ++ M+  +  LL  F       K+R+ S    LPPGP   PL+GN+ Q+G NP  ++
Sbjct: 3   LSLIQGMSLPLYFLLTLFFFFFATAKTRRSSSTGTLPPGPPILPLVGNIFQLGFNPHRSL 62

Query: 67  THFSTLHGPLISLRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSL 126
             FS  +GP++SL+LG   AVV SSP AA E LRTHD  +S R  F          + S+
Sbjct: 63  AAFSKTYGPIMSLKLGRLTAVVISSPEAAKEALRTHDHVMSAR-TFNDALRAFDHHKHSI 122

Query: 127 LLSPECDEHWKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDV 186
           +  P     W+FL+ T    L S + LD+  +LR  K   LV  +    E GE + +   
Sbjct: 123 VWIPP-SARWRFLKKTITKYLLSPQNLDAIQSLRMRKVEELVSLVNEFRERGEAIDLARA 182

Query: 187 AFTTVFNTMTNVIFSKDLMEYEGD-GAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQG 246
           +F T FN ++N +FS DL  Y+ +  +Y F        ++  +PN+ D++  +  LDLQG
Sbjct: 183 SFVTSFNIISNALFSVDLATYDSNSSSYEFHNTVVHLTDIAGIPNVGDYFQYMRFLDLQG 242

Query: 247 LRKKSAECSRRVDSVYDTFIAER------RRDRDRNGGAATGPFGN-------------- 306
            RKK+  C  ++  V+  FI  R      R +++    ++     +              
Sbjct: 243 TRKKAVLCIEKLFRVFQEFIDARLAKRFSRTEKEPKEASSIDMLDSLLDLTQQNEAELTM 302

Query: 307 --------EILSASTDTTSTTVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIP 366
                   ++  A TDT S+T+EW M EL ++   M K   EI R++  QN  + ES IP
Sbjct: 303 NDLKHLLLDVFVAGTDTNSSTMEWAMTELFRSTEKMVKAQSEI-RQVIGQNGFVQESDIP 362

Query: 367 QLHYLQACVKETLRLHPPAPFLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWEN 426
            L YLQA VKETLRLHP AP L+P ++    Q+MG+ +PK+T++ VN+WAIGRD + WEN
Sbjct: 363 SLPYLQAIVKETLRLHPAAP-LIPRKSESDVQIMGFLVPKNTQVVVNVWAIGRDASVWEN 422

Query: 427 PSTFNPDRFLGSDIEFKGFDFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPN 486
           P  F P+RFL  + + KG DF  +PFGSGRR+CPG+ M +  ++++LA+L+  FDW L N
Sbjct: 423 PMKFEPERFLLRETDVKGRDFELIPFGSGRRMCPGISMALKTMHMVLASLLYSFDWKLQN 482

Query: 487 NEDPSKLDLSGKMGIVCYKEKPLSLIPKIRVTSS 490
              P  +D+S   G+  +K K L  +P  + T S
Sbjct: 483 GVVPGNIDMSETFGLTLHKAKSLCAVPVKKPTIS 512

BLAST of Spg018891 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 322.8 bits (826), Expect = 4.8e-88
Identity = 184/498 (36.95%), Postives = 273/498 (54.82%), Query Frame = 0

Query: 18  LLLLLPFLLILKLL-------KSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTL 77
           LLLL  F+L   L+       +  + +  LPPGP   P++GN+  +G +P  +    S  
Sbjct: 9   LLLLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKT 68

Query: 78  HGPLISLRLGSQLAVVASSPSAAAEILRTHDRTLSGRYIFQTVTDTHKLRRFSLLLSPEC 137
           +GP++SL+LGS   VV +SP AA E+LRTHD+ LS R     V   +  +  SL+  P  
Sbjct: 69  YGPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSARSPTNAVRSINH-QDASLVWLPSS 128

Query: 138 DEHWKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVF 197
              W+ LR    T L S + +++  ALR NK   LV +++  ++  E V I  VAF T  
Sbjct: 129 SARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDISRVAFITTL 188

Query: 198 NTMTNVIFSKDLMEYEGDGAY-GFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSA 257
           N ++N++FS DL  Y    +  G ++     ++    P+ A+++P L  LDLQG  K   
Sbjct: 189 NIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRFLDLQGNVKTFK 248

Query: 258 ECSRRVDSVY----DTFIAERRRDRDRNGGAATGPFGN---------------------E 317
            C+ R+  V+    D  IAE+    +    +      N                     +
Sbjct: 249 VCTERLVRVFRGFIDAKIAEKSSQNNPKDVSKNDFVDNLLDYKGDESELSISDIEHLLLD 308

Query: 318 ILSASTDTTSTTVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACV 377
           + +A TDT+S+T+EW M EL+KN   M K   EI   I  QN  ++ES I +L YLQA V
Sbjct: 309 MFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVI-GQNGIVEESDISKLPYLQAVV 368

Query: 378 KETLRLHPPAPFLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRF 437
           KET RLH P P L+P +A    +++G+ + KDT++ VN+WAIGRDP+ W+NPS F P+RF
Sbjct: 369 KETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVWDNPSQFEPERF 428

Query: 438 LGSDIEFKGFDFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDL 483
           LG D++ +G D+   PFG+GRRICPG+P+ +  V+L+LA+L+  FDW LP       LD+
Sbjct: 429 LGKDMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWKLPKGVLSEDLDM 488