Spg018062 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg018062 vs. NCBI nr
Match: XP_023521223.1 (neurofilament medium polypeptide-like [Cucurbita pepo subsp. pepo] >XP_023522087.1 neurofilament medium polypeptide-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 570.1 bits (1468), Expect = 1.7e-158
Identity = 330/463 (71.27%), Postives = 369/463 (79.70%), Query Frame = 0

Query: 5   FELKISPTRADDPSSTFELQLAKATATPFFWSDETPSMFILTAKLTGFARDDIVITINGS 64
           FELKISPTR DDPSSTFEL L K T+T  F+SDET SMF+LTAKL GFARDDIVITINGS
Sbjct: 7   FELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVITINGS 66

Query: 65  STRIEIGWGQPVQKMEITRWMYKKETETRRFKKIFCIPDGVVLDEIKATYNDEESILKIL 124
            TRIEI WGQPVQKMEITRW YKKETETRRFKKIF IPDGV+LDEIKATYNDE+SILKIL
Sbjct: 67  GTRIEISWGQPVQKMEITRWTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILKIL 126

Query: 125 MPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTAELETLETGKKTRLVEDDNH 184
           MPKL +GILGVGI+EVKEQE G+A FESQD +AE IPH+  +LET ETGK+T+L+ED + 
Sbjct: 127 MPKLEEGILGVGIQEVKEQEAGKATFESQDSEAEAIPHTPTDLETPETGKETKLIEDTH- 186

Query: 185 ENEEDDAANGEFGGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSESEQL 244
                   NGEFGGENA+ EAFSKIEY AET  EEIEKLK +E ED DG SNDYSESEQL
Sbjct: 187 -------GNGEFGGENAKQEAFSKIEYAAETTPEEIEKLKGNEMEDTDGGSNDYSESEQL 246

Query: 245 ENVATKTTEIEATRENVNGEIKVPKVRSMEGIEVAEETSQGTA------------HNGNI 304
           E  ATK TE E T+EN+NG+IKVPKV SMEGIEVAE TSQ TA            HNG++
Sbjct: 247 ETGATKATEYEGTKENMNGQIKVPKVTSMEGIEVAEGTSQETANHQKEKEGNAKEHNGDM 306

Query: 305 TTVINKQPDVKTQSQASIDREAQVLDREFSQPDRQTQAEQRKQETYKNVRELEELETNKE 364
            T+I K+PDVKTQSQASID+E +   R     +R T+AE  K ETY+N REL      KE
Sbjct: 307 ATMIKKKPDVKTQSQASIDQEQENFKR-----NRHTRAELGKPETYRNTREL------KE 366

Query: 365 PEDTESPYLQSPAHEHEEKGVDQQGKKIETEDD--KAREECKSPQKETHEDSSKEAKQEK 424
           PED E PYLQSPAHEHEEK VDQ+GK  +T+D+  +AREEC SPQK TH+DSSKEAKQEK
Sbjct: 367 PEDIEIPYLQSPAHEHEEKEVDQKGKSSQTKDETKEAREECNSPQKVTHQDSSKEAKQEK 426

Query: 425 KQDSKQFKMRTPIIIAGSALLASLVIVVFNLVKSKRREKNMRG 454
               KQFKMR PII+AGSAL+ASLVIVVF+LVKSK+REKNMRG
Sbjct: 427 ----KQFKMRIPIIVAGSALVASLVIVVFSLVKSKKREKNMRG 446

BLAST of Spg018062 vs. NCBI nr
Match: KAG6607636.1 (hypothetical protein SDJN03_00978, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037240.1 hypothetical protein SDJN02_00863, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 550.4 bits (1417), Expect = 1.4e-152
Identity = 318/463 (68.68%), Postives = 362/463 (78.19%), Query Frame = 0

Query: 5   FELKISPTRADDPSSTFELQLAKATATPFFWSDETPSMFILTAKLTGFARDDIVITINGS 64
           FELKISPTR DDPSSTFEL L K T+T  F+SDET SMF+LTAKL GFARDDIV+TINGS
Sbjct: 7   FELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVVTINGS 66

Query: 65  STRIEIGWGQPVQKMEITRWMYKKETETRRFKKIFCIPDGVVLDEIKATYNDEESILKIL 124
            TRIEI WGQPVQKMEITRW YKKETETRRFKKIF IPDGV+LDEIKATYNDE+SILKIL
Sbjct: 67  GTRIEISWGQPVQKMEITRWTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILKIL 126

Query: 125 MPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTAELETLETGKKTRLVEDDNH 184
           MPKL +GILGVGI+EVKE+E GEA FESQD +AE IPH+  +LET ETGK+T+L+ED + 
Sbjct: 127 MPKLEEGILGVGIQEVKEKEAGEATFESQDSEAEAIPHTPTDLETPETGKETKLIEDTH- 186

Query: 185 ENEEDDAANGEFGGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSESEQL 244
                   NGE GGENA+ EAFSK+EY AET  EEIEKLK +E ED DG SNDYSESE+L
Sbjct: 187 -------GNGELGGENAKREAFSKMEYAAETTPEEIEKLKGNEMEDTDGGSNDYSESERL 246

Query: 245 ENVATKTTEIEATRENVNGEIKVPKVRSMEGIEVAEETSQGTA------------HNGNI 304
           E  ATK TE E T+EN+NG+IKVPKV   EGIEVAE TSQ TA            HNG++
Sbjct: 247 ETGATKATEYEGTKENINGQIKVPKVTITEGIEVAEGTSQETANHQKEKEGNAEEHNGDM 306

Query: 305 TTVINKQPDVKTQSQASIDREAQVLDREFSQPDRQTQAEQRKQETYKNVRELEELETNKE 364
            T+I K+PDVKTQ QASID+E +   R     +R T+ E  K ETY+N  +L      KE
Sbjct: 307 ATMIKKKPDVKTQPQASIDQEQENFKR-----NRHTRVELGKPETYRNTWKL------KE 366

Query: 365 PEDTESPYLQSPAHEHEEKGVDQQGKKIETEDD--KAREECKSPQKETHEDSSKEAKQEK 424
           PED E PYLQSPAHEHEEK VDQ+GK  +T+D+  +AREEC SPQK TH+DSSKEAKQEK
Sbjct: 367 PEDIEIPYLQSPAHEHEEKEVDQKGKSFQTKDETKEAREECNSPQKVTHQDSSKEAKQEK 426

Query: 425 KQDSKQFKMRTPIIIAGSALLASLVIVVFNLVKSKRREKNMRG 454
               KQFKMR PII+AGSAL+A+LVI VF+LVKSK+REKNMRG
Sbjct: 427 ----KQFKMRIPIIVAGSALVATLVIAVFSLVKSKKREKNMRG 446

BLAST of Spg018062 vs. NCBI nr
Match: XP_022143634.1 (uncharacterized protein LOC111013495 [Momordica charantia])

HSP 1 Score: 521.2 bits (1341), Expect = 9.2e-144
Identity = 313/478 (65.48%), Postives = 364/478 (76.15%), Query Frame = 0

Query: 1   MGMEFELKISPTRADDPSSTFELQLAKATATPFFWSDETPSMFILTAKLTGFARDDIVIT 60
           MG++FELKISP+ +    S FELQLA+ T +P FWS+ET SMFILTAKLTGF+R+DI IT
Sbjct: 34  MGLDFELKISPSSS---FSNFELQLARGTPSP-FWSNETNSMFILTAKLTGFSREDIGIT 93

Query: 61  INGSSTRIEIGWGQPVQKMEITRWMYKKETETRRFKKIFCIPDGVVLDEIKATYNDEESI 120
           ING+ TRIEIGWGQPVQ+MEITRWMYKKETETRRFKKIF IPDGVVLDEIKATYNDEESI
Sbjct: 94  INGTGTRIEIGWGQPVQRMEITRWMYKKETETRRFKKIFRIPDGVVLDEIKATYNDEESI 153

Query: 121 LKILMPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTAELETLETGKKTRLVE 180
           LKI+MPKLVDGILGVGIEEVKEQ+VGE A ESQD +AEEIP  T   E  ETG++T+ VE
Sbjct: 154 LKIVMPKLVDGILGVGIEEVKEQDVGEDALESQDSEAEEIPEVT---EGFETGRETKFVE 213

Query: 181 DDNHENEEDDAANGEFGGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSE 240
                    DAANG F G NA PE   K EY+AET QEE EKLK DE E IDGVSN YSE
Sbjct: 214 ---------DAANGVFEGANATPEPSPKREYLAETTQEETEKLKGDEMEHIDGVSNHYSE 273

Query: 241 SEQLENVATKTTEIEATREN---VNGEIKVPKVRSMEGIEVAEETSQGT----------- 300
           SE+ E + T+  E     +N   V+ EI +PKVRS++GIE+ E TS+GT           
Sbjct: 274 SEKSEKLTTEIAEAPKRWDNGGIVDSEIIIPKVRSVKGIELDEGTSKGTANHQKEKGEPN 333

Query: 301 --AHNGNITTVINKQPDVKTQSQASIDREAQVLDR-EFSQPDRQTQAE------QRKQET 360
             AHNG+I T+ NKQPD+K QSQA++DR+ +V D+ E SQP+ + QAE      Q  +ET
Sbjct: 334 SEAHNGSIATMTNKQPDIKIQSQAAVDRDTKVPDKPELSQPNGKIQAEHKIVEQQGNKET 393

Query: 361 YKNVRELEELETNKEPEDTESPYLQSPAHEHEEKGVDQQGKKIETEDD--KAREECKSPQ 420
           Y++  E +E ETN EPE   SPYLQSP HEHEEKGV+QQGK  +T+D+  +AREE  SPQ
Sbjct: 394 YRSAWESKEFETNNEPEYIASPYLQSPEHEHEEKGVNQQGKSFQTQDEIKEAREESGSPQ 453

Query: 421 KETHEDSSKEAKQEKKQDSKQFKMRTPIIIAGSALLASLVIVVFNLVKSKRREKNMRG 454
           +E HE S+KEAKQEK QDSKQFKMRTPIIIAGSALLASL IVVFN V+SK+REKNMRG
Sbjct: 454 REKHEQSTKEAKQEKIQDSKQFKMRTPIIIAGSALLASLAIVVFNFVRSKKREKNMRG 495

BLAST of Spg018062 vs. NCBI nr
Match: XP_022926215.1 (neurofilament medium polypeptide-like [Cucurbita moschata])

HSP 1 Score: 473.0 bits (1216), Expect = 2.9e-129
Identity = 275/416 (66.11%), Postives = 313/416 (75.24%), Query Frame = 0

Query: 5   FELKISPTRADDPSSTFELQLAKATATPFFWSDETPSMFILTAKLTGFARDDIVITINGS 64
           FELKISPTR DDPSSTFEL L K T+T  F+SDET SMF+LTAKL GFARDDIVITINGS
Sbjct: 7   FELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVITINGS 66

Query: 65  STRIEIGWGQPVQKMEITRWMYKKETETRRFKKIFCIPDGVVLDEIKATYNDEESILKIL 124
            TRIEI WGQPVQKMEITRW YKKETETRRFKKIF IPDGV+LDEIKATYNDE+SILKIL
Sbjct: 67  GTRIEISWGQPVQKMEITRWTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILKIL 126

Query: 125 MPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTAELETLETGKKTRLVEDDNH 184
           MPKL +GILGVGI+EVKE+E GEA FESQD +AE IPH+  +LET ETGK+T+L+ED + 
Sbjct: 127 MPKLEEGILGVGIQEVKEKEAGEATFESQDSEAEAIPHTPTDLETPETGKETKLIEDTH- 186

Query: 185 ENEEDDAANGEFGGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSESEQL 244
                   NGEFGGENA+ EAFSK+EY AET  EEIEKLK +E ED DG SNDYSE EQL
Sbjct: 187 -------GNGEFGGENAKREAFSKMEYAAETTPEEIEKLKGNEMEDTDGGSNDYSECEQL 246

Query: 245 ENVATKTTEIEATRENVNGEIKVPKVRSMEGIEVAEETSQGTA-------------HNGN 304
           E  ATK TE E T+EN+NG+IKVPKV SMEGIEVAE TSQ TA             HNG+
Sbjct: 247 ETGATKATEYEGTKENMNGQIKVPKVTSMEGIEVAEGTSQETANHQEEKGEGKAEEHNGD 306

Query: 305 ITTVINKQPDVKTQSQASIDREAQVLDREFSQPDRQTQAEQRKQETYKNVRELEELETNK 364
           I T+I K+ DVKTQ QASID+EAQ L++E  + +R T+AE  K ETY+N REL      K
Sbjct: 307 IATMIKKKLDVKTQPQASIDQEAQTLEQENFKRNRHTRAELGKLETYRNTREL------K 366

Query: 365 EPEDTESPYLQSPAHEHEEKGVDQQGK--------KIETEDDKAREECKSPQKETH 400
           EPED E PYLQSPAHEHEEK +  + K        K++T  D+ R +     K+ H
Sbjct: 367 EPEDIEIPYLQSPAHEHEEKEISDEKKTTQSGHTTKVKTGRDEVRFQVMGMSKQGH 408

BLAST of Spg018062 vs. NCBI nr
Match: XP_022981685.1 (uncharacterized protein LOC111480753 [Cucurbita maxima])

HSP 1 Score: 462.6 bits (1189), Expect = 3.9e-126
Identity = 266/379 (70.18%), Postives = 295/379 (77.84%), Query Frame = 0

Query: 5   FELKISPTRADDPSSTFELQLAKATATPFFWSDETPSMFILTAKLTGFARDDIVITINGS 64
           FELKISPTR DDPSSTFEL L K T+T  F+SDET SMF+LTAKL GFARDDIVITINGS
Sbjct: 7   FELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVITINGS 66

Query: 65  STRIEIGWGQPVQKMEITRWMYKKETETRRFKKIFCIPDGVVLDEIKATYNDEESILKIL 124
            T+IEI WGQPVQKMEITR  YKKETETRRFKKIF IPDGV+LDEIKATYNDE+SILKIL
Sbjct: 67  GTQIEISWGQPVQKMEITRLTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILKIL 126

Query: 125 MPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTAELETLETGKKTRLVEDDNH 184
           MPKLV+GILGVGIE VKEQE G+A FESQD +AE IPH+  +LET ETGKKT+LVED + 
Sbjct: 127 MPKLVEGILGVGIEGVKEQEAGDATFESQDSEAEAIPHAPTDLETPETGKKTKLVEDTH- 186

Query: 185 ENEEDDAANGEFGGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSESEQL 244
                   NGEFGGENA+ EAFSKIEY AET  EEIEKLK +E ED DG SNDYSESEQL
Sbjct: 187 -------GNGEFGGENAKQEAFSKIEYAAETTPEEIEKLKGNEMEDTDGGSNDYSESEQL 246

Query: 245 ENVATKTTEIEATRENVNGEIKVPKVRSMEGIEVAEETSQGTA------------HNGNI 304
           E  ATK TE E T+EN+NG+I+VPKV SMEGIEVAE TSQ TA            HNG++
Sbjct: 247 ETGATKATEYEGTKENINGQIQVPKVTSMEGIEVAEGTSQETANHQKEKEGNAEEHNGDM 306

Query: 305 TTVINKQPDVKTQSQASIDREAQVLDREFSQPDRQTQAEQRKQETYKNVRELEELETNKE 364
            T+I K+PDVKTQ QASID+E     +E  + +R T+A   K ETY+N REL      KE
Sbjct: 307 ATMIKKKPDVKTQPQASIDQE-----QENFKQNRHTRAGLGKPETYRNTREL------KE 366

Query: 365 PEDTESPYLQSPAHEHEEK 372
           PED E PYLQSPAHEHEEK
Sbjct: 367 PEDIEIPYLQSPAHEHEEK 366

BLAST of Spg018062 vs. ExPASy TrEMBL
Match: A0A6J1CPW6 (uncharacterized protein LOC111013495 OS=Momordica charantia OX=3673 GN=LOC111013495 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 4.4e-144
Identity = 313/478 (65.48%), Postives = 364/478 (76.15%), Query Frame = 0

Query: 1   MGMEFELKISPTRADDPSSTFELQLAKATATPFFWSDETPSMFILTAKLTGFARDDIVIT 60
           MG++FELKISP+ +    S FELQLA+ T +P FWS+ET SMFILTAKLTGF+R+DI IT
Sbjct: 34  MGLDFELKISPSSS---FSNFELQLARGTPSP-FWSNETNSMFILTAKLTGFSREDIGIT 93

Query: 61  INGSSTRIEIGWGQPVQKMEITRWMYKKETETRRFKKIFCIPDGVVLDEIKATYNDEESI 120
           ING+ TRIEIGWGQPVQ+MEITRWMYKKETETRRFKKIF IPDGVVLDEIKATYNDEESI
Sbjct: 94  INGTGTRIEIGWGQPVQRMEITRWMYKKETETRRFKKIFRIPDGVVLDEIKATYNDEESI 153

Query: 121 LKILMPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTAELETLETGKKTRLVE 180
           LKI+MPKLVDGILGVGIEEVKEQ+VGE A ESQD +AEEIP  T   E  ETG++T+ VE
Sbjct: 154 LKIVMPKLVDGILGVGIEEVKEQDVGEDALESQDSEAEEIPEVT---EGFETGRETKFVE 213

Query: 181 DDNHENEEDDAANGEFGGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSE 240
                    DAANG F G NA PE   K EY+AET QEE EKLK DE E IDGVSN YSE
Sbjct: 214 ---------DAANGVFEGANATPEPSPKREYLAETTQEETEKLKGDEMEHIDGVSNHYSE 273

Query: 241 SEQLENVATKTTEIEATREN---VNGEIKVPKVRSMEGIEVAEETSQGT----------- 300
           SE+ E + T+  E     +N   V+ EI +PKVRS++GIE+ E TS+GT           
Sbjct: 274 SEKSEKLTTEIAEAPKRWDNGGIVDSEIIIPKVRSVKGIELDEGTSKGTANHQKEKGEPN 333

Query: 301 --AHNGNITTVINKQPDVKTQSQASIDREAQVLDR-EFSQPDRQTQAE------QRKQET 360
             AHNG+I T+ NKQPD+K QSQA++DR+ +V D+ E SQP+ + QAE      Q  +ET
Sbjct: 334 SEAHNGSIATMTNKQPDIKIQSQAAVDRDTKVPDKPELSQPNGKIQAEHKIVEQQGNKET 393

Query: 361 YKNVRELEELETNKEPEDTESPYLQSPAHEHEEKGVDQQGKKIETEDD--KAREECKSPQ 420
           Y++  E +E ETN EPE   SPYLQSP HEHEEKGV+QQGK  +T+D+  +AREE  SPQ
Sbjct: 394 YRSAWESKEFETNNEPEYIASPYLQSPEHEHEEKGVNQQGKSFQTQDEIKEAREESGSPQ 453

Query: 421 KETHEDSSKEAKQEKKQDSKQFKMRTPIIIAGSALLASLVIVVFNLVKSKRREKNMRG 454
           +E HE S+KEAKQEK QDSKQFKMRTPIIIAGSALLASL IVVFN V+SK+REKNMRG
Sbjct: 454 REKHEQSTKEAKQEKIQDSKQFKMRTPIIIAGSALLASLAIVVFNFVRSKKREKNMRG 495

BLAST of Spg018062 vs. ExPASy TrEMBL
Match: A0A6J1EKH3 (neurofilament medium polypeptide-like OS=Cucurbita moschata OX=3662 GN=LOC111433395 PE=3 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 1.4e-129
Identity = 275/416 (66.11%), Postives = 313/416 (75.24%), Query Frame = 0

Query: 5   FELKISPTRADDPSSTFELQLAKATATPFFWSDETPSMFILTAKLTGFARDDIVITINGS 64
           FELKISPTR DDPSSTFEL L K T+T  F+SDET SMF+LTAKL GFARDDIVITINGS
Sbjct: 7   FELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVITINGS 66

Query: 65  STRIEIGWGQPVQKMEITRWMYKKETETRRFKKIFCIPDGVVLDEIKATYNDEESILKIL 124
            TRIEI WGQPVQKMEITRW YKKETETRRFKKIF IPDGV+LDEIKATYNDE+SILKIL
Sbjct: 67  GTRIEISWGQPVQKMEITRWTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILKIL 126

Query: 125 MPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTAELETLETGKKTRLVEDDNH 184
           MPKL +GILGVGI+EVKE+E GEA FESQD +AE IPH+  +LET ETGK+T+L+ED + 
Sbjct: 127 MPKLEEGILGVGIQEVKEKEAGEATFESQDSEAEAIPHTPTDLETPETGKETKLIEDTH- 186

Query: 185 ENEEDDAANGEFGGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSESEQL 244
                   NGEFGGENA+ EAFSK+EY AET  EEIEKLK +E ED DG SNDYSE EQL
Sbjct: 187 -------GNGEFGGENAKREAFSKMEYAAETTPEEIEKLKGNEMEDTDGGSNDYSECEQL 246

Query: 245 ENVATKTTEIEATRENVNGEIKVPKVRSMEGIEVAEETSQGTA-------------HNGN 304
           E  ATK TE E T+EN+NG+IKVPKV SMEGIEVAE TSQ TA             HNG+
Sbjct: 247 ETGATKATEYEGTKENMNGQIKVPKVTSMEGIEVAEGTSQETANHQEEKGEGKAEEHNGD 306

Query: 305 ITTVINKQPDVKTQSQASIDREAQVLDREFSQPDRQTQAEQRKQETYKNVRELEELETNK 364
           I T+I K+ DVKTQ QASID+EAQ L++E  + +R T+AE  K ETY+N REL      K
Sbjct: 307 IATMIKKKLDVKTQPQASIDQEAQTLEQENFKRNRHTRAELGKLETYRNTREL------K 366

Query: 365 EPEDTESPYLQSPAHEHEEKGVDQQGK--------KIETEDDKAREECKSPQKETH 400
           EPED E PYLQSPAHEHEEK +  + K        K++T  D+ R +     K+ H
Sbjct: 367 EPEDIEIPYLQSPAHEHEEKEISDEKKTTQSGHTTKVKTGRDEVRFQVMGMSKQGH 408

BLAST of Spg018062 vs. ExPASy TrEMBL
Match: A0A6J1J0B1 (uncharacterized protein LOC111480753 OS=Cucurbita maxima OX=3661 GN=LOC111480753 PE=3 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 1.9e-126
Identity = 266/379 (70.18%), Postives = 295/379 (77.84%), Query Frame = 0

Query: 5   FELKISPTRADDPSSTFELQLAKATATPFFWSDETPSMFILTAKLTGFARDDIVITINGS 64
           FELKISPTR DDPSSTFEL L K T+T  F+SDET SMF+LTAKL GFARDDIVITINGS
Sbjct: 7   FELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVITINGS 66

Query: 65  STRIEIGWGQPVQKMEITRWMYKKETETRRFKKIFCIPDGVVLDEIKATYNDEESILKIL 124
            T+IEI WGQPVQKMEITR  YKKETETRRFKKIF IPDGV+LDEIKATYNDE+SILKIL
Sbjct: 67  GTQIEISWGQPVQKMEITRLTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILKIL 126

Query: 125 MPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTAELETLETGKKTRLVEDDNH 184
           MPKLV+GILGVGIE VKEQE G+A FESQD +AE IPH+  +LET ETGKKT+LVED + 
Sbjct: 127 MPKLVEGILGVGIEGVKEQEAGDATFESQDSEAEAIPHAPTDLETPETGKKTKLVEDTH- 186

Query: 185 ENEEDDAANGEFGGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSESEQL 244
                   NGEFGGENA+ EAFSKIEY AET  EEIEKLK +E ED DG SNDYSESEQL
Sbjct: 187 -------GNGEFGGENAKQEAFSKIEYAAETTPEEIEKLKGNEMEDTDGGSNDYSESEQL 246

Query: 245 ENVATKTTEIEATRENVNGEIKVPKVRSMEGIEVAEETSQGTA------------HNGNI 304
           E  ATK TE E T+EN+NG+I+VPKV SMEGIEVAE TSQ TA            HNG++
Sbjct: 247 ETGATKATEYEGTKENINGQIQVPKVTSMEGIEVAEGTSQETANHQKEKEGNAEEHNGDM 306

Query: 305 TTVINKQPDVKTQSQASIDREAQVLDREFSQPDRQTQAEQRKQETYKNVRELEELETNKE 364
            T+I K+PDVKTQ QASID+E     +E  + +R T+A   K ETY+N REL      KE
Sbjct: 307 ATMIKKKPDVKTQPQASIDQE-----QENFKQNRHTRAGLGKPETYRNTREL------KE 366

Query: 365 PEDTESPYLQSPAHEHEEK 372
           PED E PYLQSPAHEHEEK
Sbjct: 367 PEDIEIPYLQSPAHEHEEK 366

BLAST of Spg018062 vs. ExPASy TrEMBL
Match: A0A5A7UWU5 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold518G00820 PE=4 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 8.1e-45
Identity = 178/472 (37.71%), Postives = 226/472 (47.88%), Query Frame = 0

Query: 1   MGMEFELKISPTRADDPSSTFELQLAKATATPFFWSDETPSMFILTAKLTGFARDDIVIT 60
           MG +FELKI PT  DD SSTFELQLA+ +AT        P    L   + G       +T
Sbjct: 49  MGSDFELKIKPTIDDDTSSTFELQLAEGSAT---LMKPIPCFATLVRNVIGTLVRKSDVT 108

Query: 61  INGSSTRIEIGWGQPVQKMEITRWMYKKETETRRFKKIFCIPDGVVLDEIKATYNDEESI 120
           +             P Q++    W     +E  ++  +  +     ++ IK    D + +
Sbjct: 109 L-------------PPQQLAYGVWF----SEVLKYLTMLSMSTHFGVEPIK----DSDQV 168

Query: 121 LKILMPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAE-----EIPHSTAELETLETGKK 180
               +P+                        S D D       ++      L  +   +K
Sbjct: 169 WPSEVPR------------------------SNDRDRSCLRLWKLAERPTWLTVIMKRRK 228

Query: 181 TRLVEDDNHENEEDDAANGEFGGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVS 240
             L+ +             +   +NAE E   KIEYVAE   +EIEKLK  E ++     
Sbjct: 229 PMLLME-------------QLKKKNAETERLHKIEYVAEIQPDEIEKLKGSEMDE----- 288

Query: 241 NDYSESEQLENVATKTTEIEATRENVNGEIKVPKVRSMEGIEVAEETSQGT--------- 300
                                       +I VPK+RS+EGIEVAE TS+GT         
Sbjct: 289 ----------------------------DIDVPKIRSVEGIEVAEVTSKGTASHKKETQT 348

Query: 301 ---AHNGNITTVINKQPDVKTQSQASIDREAQVLDREFSQPDRQTQAEQRKQETYKNVRE 360
              A NG+  T+INK+P VKTQSQASIDREA                           RE
Sbjct: 349 NAEARNGDKATMINKKPYVKTQSQASIDREA---------------------------RE 394

Query: 361 LEELETNKEPEDTESPYLQSPAHEHEEKGVDQQGKKIET--EDDKAREECKSPQKETHED 420
           L+ELET+KEPED  SPYLQ PA++H+EKG D++G+  +T  E   AREECKS    THED
Sbjct: 409 LKELETDKEPEDIGSPYLQRPANQHDEKGADRKGRSFQTKYEVKDAREECKS----THED 394

Query: 421 SSKEAKQEKKQDSKQFKMRTPIIIAGSALLASLVIVVFNLVKSKRREKNMRG 454
           SSKEAKQEKKQDSKQ KMRT IIIAGSALLASLV V FNLVKSK  EKN++G
Sbjct: 469 SSKEAKQEKKQDSKQLKMRTSIIIAGSALLASLVTVAFNLVKSK-TEKNVKG 394

BLAST of Spg018062 vs. ExPASy TrEMBL
Match: A0A5D3CSD6 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1081G00020 PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 4.4e-43
Identity = 138/269 (51.30%), Postives = 160/269 (59.48%), Query Frame = 0

Query: 199 ENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSESEQLENVATKTTEIEATR 258
           +NAE E   KIEYVAE   +EIEKLK  E ++                            
Sbjct: 37  KNAETERLHKIEYVAEIQPDEIEKLKGSEMDE---------------------------- 96

Query: 259 ENVNGEIKVPKVRSMEGIEVAEETSQGT------------AHNGNITTVINKQPDVKTQS 318
                +I VPK+RS+EGIEVAE TS+GT            A NG+  T+INK+P VKTQS
Sbjct: 97  -----DIDVPKIRSVEGIEVAEVTSKGTASHKKETQTNAEARNGDKATMINKKPYVKTQS 156

Query: 319 QASIDREAQVLDREFSQPDRQTQAEQRKQETYKNVRELEELETNKEPEDTESPYLQSPAH 378
           QASIDREA                           REL+ELET+KEPED  SPYLQ PA+
Sbjct: 157 QASIDREA---------------------------RELKELETDKEPEDIGSPYLQRPAN 216

Query: 379 EHEEKGVDQQGKKIET--EDDKAREECKSPQKETHEDSSKEAKQEKKQDSKQFKMRTPII 438
           +H+EKG D++G+  +T  E   AREECKS    THEDSSKEAKQEKKQDSKQ KMRT II
Sbjct: 217 QHDEKGADRKGRSFQTKYEVKDAREECKS----THEDSSKEAKQEKKQDSKQLKMRTSII 240

Query: 439 IAGSALLASLVIVVFNLVKSKRREKNMRG 454
           IAGSALLASLV V FNLVKSK  EKN++G
Sbjct: 277 IAGSALLASLVTVAFNLVKSK-TEKNVKG 240

BLAST of Spg018062 vs. TAIR 10
Match: AT1G76770.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 104.4 bits (259), Expect = 2.5e-22
Identity = 73/205 (35.61%), Postives = 113/205 (55.12%), Query Frame = 0

Query: 1   MGMEFELKISPTRADDPSSTFELQLAKATATPFFWSDETPSMFILTAKLTGFARDDIVIT 60
           M +E  LKI+ T+ DD SS+ + ++A+ +      S ET S+F L   L GF +D I I 
Sbjct: 1   MELELGLKITRTK-DDVSSSTDFRVARDSFGHRSLSRETNSVFFLILHLKGFKKDGIDIE 60

Query: 61  INGSSTRIEIGWGQPVQKMEITRWM-YKKETETRRFKKIFCIPDGVVLDEIKATYNDEES 120
           IN     I+I   + V++M + +W+ +KKETE + FKK+F IPD V+LD+IKA +N+E+ 
Sbjct: 61  INKEGNLIKISGRKQVEEMVLVKWVEWKKETEIKEFKKVFRIPDIVILDKIKARFNEEDG 120

Query: 121 ILKILMPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTAELETLETGKKTRLV 180
            L + MPK V GI G+ IEE  E+E  +     +  + +  P    + ET +  ++    
Sbjct: 121 TLTVTMPKKVKGITGLKIEEEDEEEEMKEPIVEEKTEEKTEPEEEIKEET-KPEEENEEA 180

Query: 181 EDDNHENEEDDAANGEFGGENAEPE 205
           E+   E EE+    G    E  + E
Sbjct: 181 EEPQREEEEEVVEEGTRDHEGKKEE 203

BLAST of Spg018062 vs. TAIR 10
Match: AT1G76780.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 43.5 bits (101), Expect = 5.2e-04
Identity = 97/390 (24.87%), Postives = 160/390 (41.03%), Query Frame = 0

Query: 79  MEITRWM-YKKETETRRFKKIFCIPDGVVLDEIKATYNDEESILKILMPKLVDGILGVGI 138
           M + RWM ++KE E   FKK+F IPD V LD+IKA ++D+++ L I MPK V GI G  I
Sbjct: 1   MVMIRWMAWRKEVEFSVFKKVFRIPDTVDLDKIKARFDDDDATLTITMPKRVKGISGFKI 60

Query: 139 EEVKEQEVGEAAFESQDYDAEEIPH----STAELETLETGKKTRLVEDDNHENEEDDAAN 198
           EE +E+E      E +  D  E  H       EL+     K  ++ E    E    D+  
Sbjct: 61  EEEEEEE----EEEEERVDVSEAEHKEETEKGELKDDYLEKSHQIDERIEEEKGLADSNK 120

Query: 199 GEFGGENAEPEAFSKIEYVAETPQEEIEKLK------SDETEDIDGVSNDYSESEQLENV 258
                   +P      E   +T  EE E  K      SD+++D    S  + E E+ E+ 
Sbjct: 121 ESVDSSLRKPPDIEGRECHEQTRHEEQENNKQLVQAESDDSDDFG--SRAFEEIEEQESD 180

Query: 259 ATKTTEIEATRENVNGEIKVPKVRSMEGIEVAEETSQGTA------------HNGNITTV 318
               T      E    +     +R ++GIE  E  ++ +                 +  +
Sbjct: 181 VLDRTSTSGAMEKEMTDDVGDGLRKVQGIEEPERHNEESKISEMVDGETSGHEKKKVVKM 240

Query: 319 INKQPDVKTQ--------SQASIDREAQVLDREFSQP---DRQTQAEQRKQETYKNVREL 378
             K  DVK +         + +IDR   V D E ++    D + ++++ + +    + E 
Sbjct: 241 DKKNRDVKEEVDGAMGEGFRPNIDRTQVVGDDEIAETEKNDEEFESDKLEADEVDKINEG 300

Query: 379 EELETNKEPEDTESPYLQSPAHEH--EEKGVDQQGKKIETEDDKARE------------- 419
              +  +  ED     LQ    +H  E+KG  ++    E  ++K  E             
Sbjct: 301 GNTKVRRHSEDRNLIKLQEKEEQHSKEQKGHSKEENMKELVEEKTPEAETTIRNDILGPG 360