Spg017994 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg017994 vs. NCBI nr
Match: XP_038898675.1 (DNA ligase 1 [Benincasa hispida])

HSP 1 Score: 988.8 bits (2555), Expect = 2.0e-284
Identity = 537/597 (89.95%), Postives = 566/597 (94.81%), Query Frame = 0

Query: 1   MGNRRKKKRTDDS-EPSSSSLSSSDDSDISSPQLRHRHRSGSSRRDKDKKRELRIRD--R 60
           MG+RRKKKRT DS EPSSSS SSSDDSD SS + R  HRSGSSRR+KDK+RE R RD  R
Sbjct: 1   MGSRRKKKRTVDSDEPSSSSPSSSDDSDSSSMRSRRHHRSGSSRREKDKERESRSRDRER 60

Query: 61  KRKDRNNERDRKRRKKKLKREGRRKRDDYGSESDGESSCSGDSTDNESERRKVKIVEPEQ 120
           KRKDRN ERDRKRRKKKL+RE +RKRDDYGSESD ES  SGDS D+ S+RRKVKI+EPE 
Sbjct: 61  KRKDRNRERDRKRRKKKLRRESKRKRDDYGSESDNESPHSGDSVDSRSDRRKVKIIEPEV 120

Query: 121 VVRELLKEFPDVGNDLKQLLKMIDDGQAVDINGISERTLTKRLKKLFLSLNLEESQEKVF 180
           +VR+LLKEFPDVGNDLKQLLKMIDDGQAVDI+GISER+L KRLKKLF+SLNLEESQE+VF
Sbjct: 121 IVRQLLKEFPDVGNDLKQLLKMIDDGQAVDISGISERSLAKRLKKLFVSLNLEESQERVF 180

Query: 181 LLPSNGRPTLEAIGPLIQSHLEQKRNQFDQSGTLDNKSSPPLNVETTQAREDNVSELAYP 240
           LLPSN RPTLE IGPLI++H+EQK NQ D S T+D+KSSPPLN+ETTQARED++SELAYP
Sbjct: 181 LLPSNVRPTLEVIGPLIETHMEQKSNQVDHSVTVDDKSSPPLNMETTQAREDDMSELAYP 240

Query: 241 DSEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAE 300
           D EASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEED+ELFVGPPPPAMVAEAE
Sbjct: 241 DGEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMVAEAE 300

Query: 301 TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360
           TANEAERFEEV+RIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ
Sbjct: 301 TANEAERFEEVTRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360

Query: 361 AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEE 420
           AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEE
Sbjct: 361 AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEE 420

Query: 421 LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSAKEGRGDTSVWTDTPSDRAQ 480
           LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKS+KEGRGDTSVWTDTP DRAQ
Sbjct: 421 LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPFDRAQ 480

Query: 481 KAKTSYLEAYNEAAALASNEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKKS 540
           KAK SYLEAYNEAAALASNEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKKS
Sbjct: 481 KAKMSYLEAYNEAAALASNEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKKS 540

Query: 541 KQVAEKEEEWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRNFL 595
           KQV EKE++WVGKHPWKPWDREKDLTAGRK+INFDPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 KQVVEKEDDWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL 597

BLAST of Spg017994 vs. NCBI nr
Match: XP_022926268.1 (DNA ligase 1 [Cucurbita moschata])

HSP 1 Score: 966.8 bits (2498), Expect = 8.3e-278
Identity = 527/597 (88.27%), Postives = 558/597 (93.47%), Query Frame = 0

Query: 1   MGNRRKKKRTDDSEPSSSSLSSSDDSDISSPQLRHRHRSGSSRRDKDKKRELRIRD--RK 60
           MG+ RKKKRT+ SEPSSSS SSSD+SD SS + R   RSGSSRR+KDKKRE RIRD  RK
Sbjct: 1   MGSGRKKKRTECSEPSSSSPSSSDNSDSSSMRSRRHRRSGSSRREKDKKRESRIRDRERK 60

Query: 61  RKDRNNERDRKRRKKKLKREGRRKRDDYGSESDGESSCSGDSTDNESERRKVKIVEPEQV 120
           RKDRN ERDRK RKKKL+RE +RKRDDYGSESD ESS SG+S+D+E ERRK++ VEPE V
Sbjct: 61  RKDRNRERDRKMRKKKLRREAKRKRDDYGSESDAESSRSGNSSDSEFERRKLRSVEPEVV 120

Query: 121 VRELLKEFPDVGNDLKQLLKMIDDGQAVDINGISERTLTKRLKKLFLSLNLEESQEKVFL 180
           VRELLKEFPDVGNDLKQLL MIDDGQAVDI+GISERTL KRLKKLFLSLNLEESQE+VFL
Sbjct: 121 VRELLKEFPDVGNDLKQLLSMIDDGQAVDISGISERTLAKRLKKLFLSLNLEESQERVFL 180

Query: 181 LPSNGRPTLEAIGPLIQSHLEQKRNQFDQSGTLDNKSSPPLNVETTQAREDNVSELAYPD 240
           LPSNG PTLEAIGPLIQ+H+EQK+NQFD S T DNKSSPPLN ETTQ+ +DNVS+L +PD
Sbjct: 181 LPSNGHPTLEAIGPLIQTHMEQKKNQFDHSVTPDNKSSPPLNTETTQSSKDNVSDLTHPD 240

Query: 241 SEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAET 300
            EA+GPKRRMLGPEMPSAELLAAAAKLTEAQTELRE ELEED+ELFVGPPPPAMVAEAET
Sbjct: 241 GEATGPKRRMLGPEMPSAELLAAAAKLTEAQTELREVELEEDHELFVGPPPPAMVAEAET 300

Query: 301 ANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360
           ANEAERFEEV+RIMDVEGDSPYDI GLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA
Sbjct: 301 ANEAERFEEVARIMDVEGDSPYDIFGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360

Query: 361 FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEEL 420
           FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGD+EL
Sbjct: 361 FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDDEL 420

Query: 421 LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSAKEGRGDTSVWTDTPSDRAQK 480
           LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSK +KEGRGDTSVWTDTPSDRAQK
Sbjct: 421 LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKGSKEGRGDTSVWTDTPSDRAQK 480

Query: 481 AKTSYLEAYNEAAALASNEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKKSK 540
           AKTSYLEAYNEAAALAS+EEAKK SSESALVDKYN+EKRSK L+EKHREGA+S GKKKSK
Sbjct: 481 AKTSYLEAYNEAAALASSEEAKKNSSESALVDKYNREKRSKPLIEKHREGARSSGKKKSK 540

Query: 541 -QVAEKEEEWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRNFL 595
            QV EKEEEWVGKHPWKPWDREKDLTAGRK+IN DPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 QQVGEKEEEWVGKHPWKPWDREKDLTAGRKTINLDPENMAQGLGSRFSSGSYQRNFL 597

BLAST of Spg017994 vs. NCBI nr
Match: KAG7011072.1 (hypothetical protein SDJN02_27870, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 966.5 bits (2497), Expect = 1.1e-277
Identity = 529/598 (88.46%), Postives = 559/598 (93.48%), Query Frame = 0

Query: 1   MGNRRKKKRTDDSEPSSSSLSSSDDSDISSPQLRHRHRSGSSRRDKDKKRELRIRD--RK 60
           MG+ RKKKRT+ SEPSSSS SSSD+SD SS + R   RSGSSRR+KDKKRE RIRD  RK
Sbjct: 119 MGSGRKKKRTECSEPSSSSPSSSDNSDSSSMRSRRHRRSGSSRREKDKKRESRIRDRERK 178

Query: 61  RKDRNNERDRKRRKKKLKREGRRKRDDYGSESDGESSCSGDSTDNESERRKVKIVEPEQV 120
           RKDRN ERDRK RKKKL+RE +RKRDDYGSESD ESS SG+S+D+E ERRK++ VEPE V
Sbjct: 179 RKDRNRERDRKMRKKKLRREAKRKRDDYGSESDAESSRSGNSSDSEFERRKLRSVEPEVV 238

Query: 121 VRELLKEFPDVGNDLKQLLKMIDDGQAVDINGISERTLTKRLKKLFLSLNLEESQEKVFL 180
           VRELLKEFPDVGNDLKQLL MIDDGQAVDI+GISERTL KRLKKLFLSLNLEESQE+VFL
Sbjct: 239 VRELLKEFPDVGNDLKQLLSMIDDGQAVDISGISERTLAKRLKKLFLSLNLEESQERVFL 298

Query: 181 LPSNGRPTLEAIGPLIQSHLEQKRNQFDQSGTLDNKSSPPLNVETTQAREDNVSELAYPD 240
           LPSNG PTLEAIGPLIQ+H+EQK+NQFD S T DNKSSPPLN ETTQ+ +DNVS+L +PD
Sbjct: 299 LPSNGHPTLEAIGPLIQTHMEQKKNQFDHSVTPDNKSSPPLNTETTQSSKDNVSDLTHPD 358

Query: 241 SEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAET 300
            EA+GPKRRMLGPEMPSAELLAAAAKLTEAQTELRE ELEED+ELFVGPPPPAMVAEAET
Sbjct: 359 GEATGPKRRMLGPEMPSAELLAAAAKLTEAQTELREVELEEDHELFVGPPPPAMVAEAET 418

Query: 301 ANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360
           ANEAERFEEV+RIMDVEGDSPYDI GLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA
Sbjct: 419 ANEAERFEEVARIMDVEGDSPYDIFGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 478

Query: 361 FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEEL 420
           FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGD+EL
Sbjct: 479 FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDDEL 538

Query: 421 LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSAKEGRGDTSVWTDTPSDRAQK 480
           LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSK +KEGRGDTSVWTDTPSDRAQK
Sbjct: 539 LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKGSKEGRGDTSVWTDTPSDRAQK 598

Query: 481 AKTSYLEAYNEAAALASNEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSR-GKKKS 540
           AKTSYLEAYNEAAALASNEEAKK SSESALVDKYN+EKRSK L+EKHREGA+S  GKKKS
Sbjct: 599 AKTSYLEAYNEAAALASNEEAKKNSSESALVDKYNREKRSKPLIEKHREGARSSGGKKKS 658

Query: 541 K-QVAEKEEEWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRNFL 595
           K QV EKEEEWVGKHPWKPWDREKDLTAGRK+INFDPENMAQGLGSRFSSGSYQRNFL
Sbjct: 659 KQQVGEKEEEWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL 716

BLAST of Spg017994 vs. NCBI nr
Match: XP_023521008.1 (DNA ligase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 964.5 bits (2492), Expect = 4.1e-277
Identity = 527/598 (88.13%), Postives = 559/598 (93.48%), Query Frame = 0

Query: 1   MGNRRKKKRTDDSEPSSSSLSSSDDSDISSPQLRHRHRSGSSRRDKDKKRELRIRD--RK 60
           MG+ RKKKRT+ SEPSSSS SSSD+SD SS + R   RSGSSRR+KDKKRE RIRD  RK
Sbjct: 1   MGSGRKKKRTEYSEPSSSSPSSSDNSDSSSMRSRRHRRSGSSRREKDKKRESRIRDRERK 60

Query: 61  RKDRNNERDRKRRKKKLKREGRRKRDDYGSESDGESSCSGDSTDNESERRKVKIVEPEQV 120
           RKDRN ERDRK RKKKL+RE +RKRDDYGSESD ESS SG+S+D+E ERRK++ +EPE V
Sbjct: 61  RKDRNRERDRKMRKKKLRREAKRKRDDYGSESDAESSRSGNSSDSEFERRKLRSIEPEVV 120

Query: 121 VRELLKEFPDVGNDLKQLLKMIDDGQAVDINGISERTLTKRLKKLFLSLNLEESQEKVFL 180
           VRELLKEFPDVGNDLKQLL MIDDGQAVDI+GISERTL KRLKKLFLSLNLEESQE+VFL
Sbjct: 121 VRELLKEFPDVGNDLKQLLSMIDDGQAVDISGISERTLAKRLKKLFLSLNLEESQERVFL 180

Query: 181 LPSNGRPTLEAIGPLIQSHLEQKRNQFDQSGTLDNKSSPPLNVETTQAREDNVSELAYPD 240
           LPSNG PTLEAIGPLIQ+H+EQK+NQFD S T DNKSSPPLN ETTQ+ ++NVS+L +PD
Sbjct: 181 LPSNGHPTLEAIGPLIQTHMEQKKNQFDHSVTPDNKSSPPLNTETTQSSKENVSDLTHPD 240

Query: 241 SEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAET 300
            EA+GPKRRMLGPEMPSAELLAAAAKLTEAQTELRE ELEED+ELFVGPPPPAMVAEAET
Sbjct: 241 GEATGPKRRMLGPEMPSAELLAAAAKLTEAQTELREVELEEDHELFVGPPPPAMVAEAET 300

Query: 301 ANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360
           ANEAERFEEV+RIMDVEGDSPYDI GLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA
Sbjct: 301 ANEAERFEEVARIMDVEGDSPYDIFGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360

Query: 361 FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEEL 420
           FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGD+EL
Sbjct: 361 FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDDEL 420

Query: 421 LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSAKEGRGDTSVWTDTPSDRAQK 480
           LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSK +KEGRGDTSVWTDTPSDRAQK
Sbjct: 421 LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKGSKEGRGDTSVWTDTPSDRAQK 480

Query: 481 AKTSYLEAYNEAAALASNEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSR-GKKKS 540
           AKTSYLEAYNEAAALASNEEAKK SSESALVDKYN+EKRSK L+EKHREGA+S  GKKKS
Sbjct: 481 AKTSYLEAYNEAAALASNEEAKKNSSESALVDKYNREKRSKPLIEKHREGARSSGGKKKS 540

Query: 541 K-QVAEKEEEWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRNFL 595
           K QV EKEEEWVGKHPWKPWDREKDLTAGRK+INFDPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 KQQVGEKEEEWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL 598

BLAST of Spg017994 vs. NCBI nr
Match: KAG6607621.1 (hypothetical protein SDJN03_00963, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 964.1 bits (2491), Expect = 5.4e-277
Identity = 528/598 (88.29%), Postives = 558/598 (93.31%), Query Frame = 0

Query: 1   MGNRRKKKRTDDSEPSSSSLSSSDDSDISSPQLRHRHRSGSSRRDKDKKRELRIRD--RK 60
           MG+ RKKKRT+ SEPSSSS SSSD+SD SS + R   RSGSSRR+KDKKRE RIRD  RK
Sbjct: 1   MGSGRKKKRTECSEPSSSSPSSSDNSDSSSMRSRRHRRSGSSRREKDKKRESRIRDRERK 60

Query: 61  RKDRNNERDRKRRKKKLKREGRRKRDDYGSESDGESSCSGDSTDNESERRKVKIVEPEQV 120
           RKDRN ERDRK RKKKL+RE +RKRDDYGSESD ESS SG+S+D+E ERRK++ VEPE V
Sbjct: 61  RKDRNRERDRKMRKKKLRREAKRKRDDYGSESDAESSRSGNSSDSEFERRKLRSVEPEVV 120

Query: 121 VRELLKEFPDVGNDLKQLLKMIDDGQAVDINGISERTLTKRLKKLFLSLNLEESQEKVFL 180
           VRELLKEFPDVGNDLKQLL MIDDGQAVDI+GISERTL KRLKKLFLSLNLEESQE+VFL
Sbjct: 121 VRELLKEFPDVGNDLKQLLSMIDDGQAVDISGISERTLAKRLKKLFLSLNLEESQERVFL 180

Query: 181 LPSNGRPTLEAIGPLIQSHLEQKRNQFDQSGTLDNKSSPPLNVETTQAREDNVSELAYPD 240
           LPSNG PTLEAIGPLIQ+H+EQK+NQFD S T DNKSSPPLN ETTQ+ +DNVS+L +PD
Sbjct: 181 LPSNGHPTLEAIGPLIQTHMEQKKNQFDHSVTPDNKSSPPLNTETTQSSKDNVSDLTHPD 240

Query: 241 SEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAET 300
            EA+GPKRRMLGPEMPSAELLAAAAKLTEAQTELRE ELEED+ELFVGPPPPAMVAEAET
Sbjct: 241 GEATGPKRRMLGPEMPSAELLAAAAKLTEAQTELREVELEEDHELFVGPPPPAMVAEAET 300

Query: 301 ANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360
           ANEAERFEEV+RIMDVEGDSPYDI GLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA
Sbjct: 301 ANEAERFEEVARIMDVEGDSPYDIFGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360

Query: 361 FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEEL 420
           FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGD+EL
Sbjct: 361 FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDDEL 420

Query: 421 LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSAKEGRGDTSVWTDTPSDRAQK 480
           LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSK +KEGRGDTSVWTDTPSDRAQK
Sbjct: 421 LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKGSKEGRGDTSVWTDTPSDRAQK 480

Query: 481 AKTSYLEAYNEAAALASNEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSR-GKKKS 540
           AKTSYLEAYNEAAALASNEEAKK SSESALVDKYN+EKRSK L+EKHREGA+S  GKKKS
Sbjct: 481 AKTSYLEAYNEAAALASNEEAKKNSSESALVDKYNREKRSKPLIEKHREGARSSGGKKKS 540

Query: 541 K-QVAEKEEEWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRNFL 595
           K QV EKEEEWVGKHPWKPWDREKDLTAGRK+IN DPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 KQQVGEKEEEWVGKHPWKPWDREKDLTAGRKTINLDPENMAQGLGSRFSSGSYQRNFL 598

BLAST of Spg017994 vs. ExPASy Swiss-Prot
Match: Q9CZJ9 (DnaJ homolog subfamily C member 18 OS=Mus musculus OX=10090 GN=Dnajc18 PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 4.5e-08
Identity = 34/74 (45.95%), Postives = 45/74 (60.81%), Query Frame = 0

Query: 320 YDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFIRLNNAFKELQDPDKRKALD 379
           YDILG++ N S E +KK Y K++L  HPDK   P A +AF  + NAF  L +PDKR   D
Sbjct: 84  YDILGVSHNASDEELKKAYKKLALKFHPDKNCAPGATEAFKAIGNAFAVLSNPDKRLRYD 143

Query: 380 DKIKFKEEQEQFKV 394
           +   + +EQ  F V
Sbjct: 144 E---YGDEQVTFTV 154

BLAST of Spg017994 vs. ExPASy Swiss-Prot
Match: Q17438 (DnaJ homolog subfamily B member 1 OS=Caenorhabditis elegans OX=6239 GN=dnj-1 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 4.5e-08
Identity = 31/82 (37.80%), Postives = 47/82 (57.32%), Query Frame = 0

Query: 298 TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 357
           T+ + E  E +    D      Y+IL +++  S ++I+K Y K++L +HPDKC  P A +
Sbjct: 122 TSEQKELVERIRHCKDY-----YEILKIDKKASDDDIRKEYRKLALKLHPDKCRAPHATE 181

Query: 358 AFIRLNNAFKELQDPDKRKALD 380
           AF  L NA+  L D DKR+  D
Sbjct: 182 AFKALGNAYAVLSDTDKRRQYD 198

BLAST of Spg017994 vs. ExPASy Swiss-Prot
Match: Q69ZC8 (GPALPP motifs-containing protein 1 OS=Mus musculus OX=10090 GN=Gpalpp1 PE=1 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 7.6e-08
Identity = 60/182 (32.97%), Postives = 86/182 (47.25%), Query Frame = 0

Query: 416 EELLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSAKEGRGDTSVWTDTPSDR 475
           +E L   DD+      R+ WMT LPPE K    +    F + A +  GD SVWTDTP+DR
Sbjct: 178 KEKLTKGDDDSSKPITRESWMTELPPEMKE-FGLGPRTFKRRADDKSGDRSVWTDTPADR 237

Query: 476 AQKAKTSYLEAYNEAAALASNEEAKKYSSE----SALVDKYNQEKRSKSLVEKHREGAKS 535
            +KAK    E      + +  +E    S      +  V  YN+ KRS+SL++ H +  KS
Sbjct: 238 ERKAK----EIQEARKSFSKKDEENILSGRDKRLAEQVSSYNESKRSESLMDIHHKKLKS 297

Query: 536 RGKKKSKQVAEKEEEWVGKHPWKPWDREKDLTAGRKSINFDPE------NMAQGLGSRFS 588
               K+ +   K +E +      P+DR+KDL   R    FD          ++ L +RFS
Sbjct: 298 ----KAAEDKNKHQERI------PFDRDKDLKVNR----FDEAQKKALIKKSRELNTRFS 340

BLAST of Spg017994 vs. ExPASy Swiss-Prot
Match: Q4V893 (GPALPP motifs-containing protein 1 OS=Rattus norvegicus OX=10116 GN=Gpalpp1 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 7.6e-08
Identity = 58/182 (31.87%), Postives = 85/182 (46.70%), Query Frame = 0

Query: 416 EELLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSAKEGRGDTSVWTDTPSDR 475
           +E L   DD+      R+ WMT LPPE K    +    F + A +  GD SVWTDTP+DR
Sbjct: 180 KEKLTKGDDDSPKPVTRESWMTELPPEMK-DFGLGPRTFKRRADDKSGDRSVWTDTPADR 239

Query: 476 AQKAKTSYLEAYNEAAALASNEEAKKYSSE----SALVDKYNQEKRSKSLVEKHREGAKS 535
            +KAK    E      +L+  +E    S      +  V  YN+ KRS+SL++ H +  K+
Sbjct: 240 ERKAK----EIQEARKSLSKKDEENMLSGRDKRLAEQVSSYNESKRSESLMDIHHKRLKT 299

Query: 536 RGKKKSKQVAEKEEEWVGKHPWKPWDREKDLTAGRKSINFDPE------NMAQGLGSRFS 588
           +  +   +  E+           P+DR+KDL   R    FD          ++ L +RFS
Sbjct: 300 KAAEDKNRHQER----------TPFDRDKDLKVNR----FDEAQKKALIKKSRELNTRFS 342

BLAST of Spg017994 vs. ExPASy Swiss-Prot
Match: P56101 (DnaJ homolog subfamily C member 5 OS=Tetronarce californica OX=7787 GN=dnajc5 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.0e-07
Identity = 32/75 (42.67%), Postives = 46/75 (61.33%), Query Frame = 0

Query: 306 EEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDK-CSHPQANQAFIRLNN 365
           ++  R +   GDS Y +LGL++N S E+IKK Y K++L  HPDK   +P+A++ F  +NN
Sbjct: 3   DQRQRSLSTSGDSLYIVLGLDKNASPEDIKKSYRKLALKYHPDKNPDNPEASEKFKEINN 62

Query: 366 AFKELQDPDKRKALD 380
           A   L D  KR   D
Sbjct: 63  AHAILTDATKRNIYD 77

BLAST of Spg017994 vs. ExPASy TrEMBL
Match: A0A6J1EHJ8 (DNA ligase 1 OS=Cucurbita moschata OX=3662 GN=LOC111433443 PE=4 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 4.0e-278
Identity = 527/597 (88.27%), Postives = 558/597 (93.47%), Query Frame = 0

Query: 1   MGNRRKKKRTDDSEPSSSSLSSSDDSDISSPQLRHRHRSGSSRRDKDKKRELRIRD--RK 60
           MG+ RKKKRT+ SEPSSSS SSSD+SD SS + R   RSGSSRR+KDKKRE RIRD  RK
Sbjct: 1   MGSGRKKKRTECSEPSSSSPSSSDNSDSSSMRSRRHRRSGSSRREKDKKRESRIRDRERK 60

Query: 61  RKDRNNERDRKRRKKKLKREGRRKRDDYGSESDGESSCSGDSTDNESERRKVKIVEPEQV 120
           RKDRN ERDRK RKKKL+RE +RKRDDYGSESD ESS SG+S+D+E ERRK++ VEPE V
Sbjct: 61  RKDRNRERDRKMRKKKLRREAKRKRDDYGSESDAESSRSGNSSDSEFERRKLRSVEPEVV 120

Query: 121 VRELLKEFPDVGNDLKQLLKMIDDGQAVDINGISERTLTKRLKKLFLSLNLEESQEKVFL 180
           VRELLKEFPDVGNDLKQLL MIDDGQAVDI+GISERTL KRLKKLFLSLNLEESQE+VFL
Sbjct: 121 VRELLKEFPDVGNDLKQLLSMIDDGQAVDISGISERTLAKRLKKLFLSLNLEESQERVFL 180

Query: 181 LPSNGRPTLEAIGPLIQSHLEQKRNQFDQSGTLDNKSSPPLNVETTQAREDNVSELAYPD 240
           LPSNG PTLEAIGPLIQ+H+EQK+NQFD S T DNKSSPPLN ETTQ+ +DNVS+L +PD
Sbjct: 181 LPSNGHPTLEAIGPLIQTHMEQKKNQFDHSVTPDNKSSPPLNTETTQSSKDNVSDLTHPD 240

Query: 241 SEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAET 300
            EA+GPKRRMLGPEMPSAELLAAAAKLTEAQTELRE ELEED+ELFVGPPPPAMVAEAET
Sbjct: 241 GEATGPKRRMLGPEMPSAELLAAAAKLTEAQTELREVELEEDHELFVGPPPPAMVAEAET 300

Query: 301 ANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360
           ANEAERFEEV+RIMDVEGDSPYDI GLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA
Sbjct: 301 ANEAERFEEVARIMDVEGDSPYDIFGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360

Query: 361 FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEEL 420
           FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGD+EL
Sbjct: 361 FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDDEL 420

Query: 421 LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSAKEGRGDTSVWTDTPSDRAQK 480
           LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSK +KEGRGDTSVWTDTPSDRAQK
Sbjct: 421 LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKGSKEGRGDTSVWTDTPSDRAQK 480

Query: 481 AKTSYLEAYNEAAALASNEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKKSK 540
           AKTSYLEAYNEAAALAS+EEAKK SSESALVDKYN+EKRSK L+EKHREGA+S GKKKSK
Sbjct: 481 AKTSYLEAYNEAAALASSEEAKKNSSESALVDKYNREKRSKPLIEKHREGARSSGKKKSK 540

Query: 541 -QVAEKEEEWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRNFL 595
            QV EKEEEWVGKHPWKPWDREKDLTAGRK+IN DPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 QQVGEKEEEWVGKHPWKPWDREKDLTAGRKTINLDPENMAQGLGSRFSSGSYQRNFL 597

BLAST of Spg017994 vs. ExPASy TrEMBL
Match: A0A1S3CFW3 (uncharacterized protein LOC103500427 OS=Cucumis melo OX=3656 GN=LOC103500427 PE=4 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.8e-276
Identity = 522/601 (86.86%), Postives = 560/601 (93.18%), Query Frame = 0

Query: 1   MGNRRKKKRTDDSE-PSSSSLSSSDDSDISSPQLRHRHRSGSSRRDKDKKRELRIRDRKR 60
           MG+RRKK RT  SE PSSSS SSSDDSD SS + R  +RS SSRR+K+K+RE RIRDR+R
Sbjct: 51  MGSRRKKNRTVGSEDPSSSSPSSSDDSDSSSMRSRRHYRSDSSRREKEKERESRIRDRER 110

Query: 61  K------DRNNERDRKRRKKKLKREGRRKRDDYGSESDGESSCSGDSTDNESERRKVKIV 120
           K      +R+ ERDRKRRKKKL+RE +RKRDD+GS+SD ESS SGDS D +S+RRKVKI+
Sbjct: 111 KRKNRNRERDRERDRKRRKKKLRRESKRKRDDFGSDSDNESSHSGDSVDTKSDRRKVKIM 170

Query: 121 EPEQVVRELLKEFPDVGNDLKQLLKMIDDGQAVDINGISERTLTKRLKKLFLSLNLEESQ 180
           EPE +VR+LLK+FPD+GNDLKQLLKMIDDGQAVDI+GIS ++LTKRLKKLFLSLNLEESQ
Sbjct: 171 EPEVIVRQLLKDFPDIGNDLKQLLKMIDDGQAVDISGISVKSLTKRLKKLFLSLNLEESQ 230

Query: 181 EKVFLLPSNGRPTLEAIGPLIQSHLEQKRNQFDQSGTLDNKSSPPLNVETTQAREDNVSE 240
           E+VFLLPS+  PTLE IGPLI+ H++QK N  D S TLD+KSSPPLN+ETTQAREDN+SE
Sbjct: 231 ERVFLLPSSVHPTLEVIGPLIEMHMDQKSNPVDDSITLDDKSSPPLNMETTQAREDNMSE 290

Query: 241 LAYPDSEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMV 300
           LAYPD EASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEED+ELFVGPPPPAMV
Sbjct: 291 LAYPDGEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMV 350

Query: 301 AEAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHP 360
           AEAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHP
Sbjct: 351 AEAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHP 410

Query: 361 QANQAFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISME 420
           QANQAFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISME
Sbjct: 411 QANQAFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISME 470

Query: 421 GDEELLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSAKEGRGDTSVWTDTPS 480
           GDEELLANMDDEV  EPKRDEWMT+LPPERKPGMTMQSSRFSKS+KEGRGDTS+WTDTPS
Sbjct: 471 GDEELLANMDDEVTAEPKRDEWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTSIWTDTPS 530

Query: 481 DRAQKAKTSYLEAYNEAAALASNEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRG 540
           DRAQKAK SYLEAYNEAAALASNEEAKKYS+ESALVDKYNQEKRSKSLVEKHREG KSRG
Sbjct: 531 DRAQKAKMSYLEAYNEAAALASNEEAKKYSAESALVDKYNQEKRSKSLVEKHREGVKSRG 590

Query: 541 KKKSKQVAEKEEEWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRNF 595
           K KSKQVAEKE++WVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRNF
Sbjct: 591 KNKSKQVAEKEDDWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRNF 650

BLAST of Spg017994 vs. ExPASy TrEMBL
Match: A0A6J1DVD5 (uncharacterized protein LOC111023459 OS=Momordica charantia OX=3673 GN=LOC111023459 PE=4 SV=1)

HSP 1 Score: 922.5 bits (2383), Expect = 8.7e-265
Identity = 507/594 (85.35%), Postives = 542/594 (91.25%), Query Frame = 0

Query: 1   MGNRRKKKRTDDSEPSSSSLSSSDDSDISSPQLRHRHRSGSSRRDKDKKRELRIRDRKRK 60
           MG+ RKK RTDDSEPSSSS SS DDSD S+ + R  HR G SRR+KDKKRE     RKRK
Sbjct: 1   MGSGRKKWRTDDSEPSSSSPSSDDDSDTSASRSRRHHRGGGSRREKDKKRE----SRKRK 60

Query: 61  DRNNERDRKRRKKKLKREGRRKRDDYGSESDGESSCSGDSTDNESERRKVKIVEPEQVVR 120
           DR  ERDRKRRKKK +REG+RKR DY SESD       D +  ESE  KV+I EPE VVR
Sbjct: 61  DRMRERDRKRRKKKSRREGKRKR-DYFSESD-------DGSSGESELEKVRI-EPEVVVR 120

Query: 121 ELLKEFPDVGNDLKQLLKMIDDGQAVDINGISERTLTKRLKKLFLSLNLEESQEKVFLLP 180
           +LLKEFPDVG+DL+QLLKMIDDGQAVDI+GISER+LTKRLKKLF SLNLEE+Q++VFLLP
Sbjct: 121 QLLKEFPDVGDDLEQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVFLLP 180

Query: 181 SNGRPTLEAIGPLIQSHLEQKRNQFDQSGTLDNKSSPPLNVETTQAREDNVSELAYPDSE 240
           SNGR TLE IGP+IQ H+EQKRNQFD S TLDNKSSPP N+ETTQAREDN+SELA PD E
Sbjct: 181 SNGRSTLELIGPIIQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACPDGE 240

Query: 241 ASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAETAN 300
           ASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAETAN
Sbjct: 241 ASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAETAN 300

Query: 301 EAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFI 360
           EAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFI
Sbjct: 301 EAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFI 360

Query: 361 RLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEELLA 420
           RLNNAFKELQDPDKRKALDDKIK KEEQEQFKVELRGMREAAKWR+SQGISM+GDEELLA
Sbjct: 361 RLNNAFKELQDPDKRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEELLA 420

Query: 421 NMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSAKEGRGDTSVWTDTPSDRAQKAK 480
           N +D+VKVEPKRDEWMT+LPPERKPG+TMQSSRFSKS+KEGRGDTSVWTDTP DRAQKAK
Sbjct: 421 NTEDDVKVEPKRDEWMTTLPPERKPGITMQSSRFSKSSKEGRGDTSVWTDTPLDRAQKAK 480

Query: 481 TSYLEAYNEAAALASNEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKKSKQV 540
           TSYLEAYNEA ALASNEEAKK+SSESALVDKYN+EKRSKSL+EKHRE  +SR KKKSKQV
Sbjct: 481 TSYLEAYNEATALASNEEAKKHSSESALVDKYNKEKRSKSLIEKHREETRSRAKKKSKQV 540

Query: 541 AEKEEEWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRNFL 595
           AEK ++WVGKHPWKPWDREKDLTAGRK++NFDPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 AEKGDDWVGKHPWKPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL 581

BLAST of Spg017994 vs. ExPASy TrEMBL
Match: A0A2C9WFD9 (J domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_02G191800 PE=4 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 2.8e-199
Identity = 407/601 (67.72%), Postives = 479/601 (79.70%), Query Frame = 0

Query: 1   MGNRRKKKRTDDSEPSSSSLSSSDDSDISSPQLRHRHR-----SGSSRRDKDKKRELRIR 60
           MG++ +KK  +D+ P+SSS S  DDS+ S  + R R +      GSSRR+ D+KRE R  
Sbjct: 1   MGSKTRKKHREDT-PTSSSNSDDDDSETSEDRRRRRRKDRRSSDGSSRRE-DRKRERRDE 60

Query: 61  DRKRKDRNNERDRKRRKKKLKREGRRKRDDYGSESD-GESSCSGDSTDNESERRKVKIVE 120
            R+++    ERDRKRRK        RK+ DY SES+ G  S SG  +D E  +R  + V+
Sbjct: 61  KRRKRKERKERDRKRRKS-------RKKRDYDSESESGSGSESGSGSDAEEPKR--QRVK 120

Query: 121 PEQVVRELLKEFPDVGNDLKQLLKMIDDGQAVDINGISERTLTKRLKKLFLSLNLEESQE 180
           PE VV+E+L EFPDVG+DLKQLL+MID GQAVDI GISE+ L K LKKLF+SLNL E  +
Sbjct: 121 PEDVVKEMLMEFPDVGDDLKQLLQMIDSGQAVDIKGISEKPLIKHLKKLFVSLNLMEKGD 180

Query: 181 KVFLLPSNGRPTLEAIGPLIQSHLEQKRNQFDQSGTLDNKSSPPLNVETTQAREDNVSEL 240
           +VFLLPSN  PTLE +GPLI++H+  K  + D     D  S PP+  E+ Q  ++N S +
Sbjct: 181 RVFLLPSNNCPTLELVGPLIEAHIRSKEQKVD-----DVHSVPPV-AESKQLSDNNNSVM 240

Query: 241 AYPDSEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVA 300
             PD +ASGP+RRM+GPEMPSAELLAAAAKLTEA  ELREAELEED+ELF+GP PPAMVA
Sbjct: 241 PAPDDDASGPRRRMIGPEMPSAELLAAAAKLTEAHAELREAELEEDDELFIGPAPPAMVA 300

Query: 301 EAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQ 360
           EAE+ANEAERFEEV+RIM VEGDSPYD++G N N S ENIKKRYWK+SLLVHPDKCSHPQ
Sbjct: 301 EAESANEAERFEEVTRIMGVEGDSPYDVVGANHNTSAENIKKRYWKLSLLVHPDKCSHPQ 360

Query: 361 ANQAFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEG 420
           A+QAFI+LN AFKELQDPDKRK +D+KIK KEEQEQFKVEL+ MREAA+WR+SQGISMEG
Sbjct: 361 AHQAFIKLNKAFKELQDPDKRKLMDEKIKLKEEQEQFKVELKAMREAAQWRRSQGISMEG 420

Query: 421 DEELLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSAKEGRGDTSVWTDTPSD 480
           D+ELLA  + EVKV PKRDEWMT+LPPERKPGMTM SS+FSK ++EGRGDTSVWTDTPSD
Sbjct: 421 DDELLA--ETEVKVAPKRDEWMTTLPPERKPGMTMHSSKFSKGSREGRGDTSVWTDTPSD 480

Query: 481 RAQKAKTSYLEAYNEAAALASNEEAKKYSSESA-LVDKYNQEKRSKSLVEKHREGAKSRG 540
           RAQKAK +YLEAYNEAAALASNEE KK SS  A LVDKYN+ KRSKSLV+KH+E A SR 
Sbjct: 481 RAQKAKMNYLEAYNEAAALASNEEEKKRSSADADLVDKYNKAKRSKSLVQKHQEEAASRS 540

Query: 541 KKKSKQVAEKEEEWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRNF 595
           KKKSK+  EK EEW G+HPWKPWDREKDL AGR++I FD +NMAQGL SRFSSG++QRNF
Sbjct: 541 KKKSKEQPEK-EEWAGQHPWKPWDREKDLVAGRQNIKFDADNMAQGLSSRFSSGNFQRNF 581

BLAST of Spg017994 vs. ExPASy TrEMBL
Match: A0A0B0MMZ6 (DnaJ subfamily C member 14 OS=Gossypium arboreum OX=29729 GN=LOC108461091 PE=4 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 1.3e-196
Identity = 398/597 (66.67%), Postives = 478/597 (80.07%), Query Frame = 0

Query: 1   MGNRRKKKRTDDSEPSSSSLSSSDDSDISSPQLRHRHRSGSSRRDKDKKRELRIRDRKRK 60
           MG + +KK  DD+  SSSS S+SDDS+ SS + RHRHR    R+D+  +RE   +  KRK
Sbjct: 1   MGRKTRKKHVDDTPTSSSSPSTSDDSE-SSLERRHRHRKVRRRKDESSRREKERKREKRK 60

Query: 61  DRNNERDRKRRKKKLKREGRRKRDDYGSESDGESSCSGDSTDNESERRKVKIVEPEQVVR 120
            +  ER+RKRRK + + + R KR DY S+S   S  S  S   E +RR    VEPE VV+
Sbjct: 61  RKEKERERKRRKSR-REDRREKRRDYESDSKSGSGSSSGSDSEEDKRR----VEPEVVVQ 120

Query: 121 ELLKEFPDVGNDLKQLLKMIDDGQAVDINGISERTLTKRLKKLFLSLNLEESQEKVFLLP 180
           ++LKEFP+VGNDLKQLL+MIDDGQAVDI GISER+L K LKKLFLSL L+E+ ++VFLL 
Sbjct: 121 QILKEFPNVGNDLKQLLQMIDDGQAVDIKGISERSLNKHLKKLFLSLKLKENGDRVFLLS 180

Query: 181 SNGRPTLEAIGPLIQSHLEQKRNQFDQSGTLDNKSSPPLNVETTQAREDNVSELAYPDSE 240
           S  RPTL+ +G LIQ+H E K  Q   SG + +  S P + E++Q  ++N S   Y D  
Sbjct: 181 SKSRPTLDVVGHLIQAHTEPKEQQRTNSGLVKDAHSIPEHAESSQVMDENNS---YRDDS 240

Query: 241 ASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAETAN 300
           A+ PKRR++GP MPSAELLAAAAKLTEAQ ELREAE+EED+ELF+GPPPPA+VAEAE+AN
Sbjct: 241 AT-PKRRVIGPAMPSAELLAAAAKLTEAQAELREAEIEEDDELFIGPPPPALVAEAESAN 300

Query: 301 EAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFI 360
           EAERFEEV+RI+ VE D PYD++G NRN + +NIKK+YWK+SL+VHPDKC+HPQA+QAFI
Sbjct: 301 EAERFEEVTRIVGVESDCPYDVIGANRNTTADNIKKKYWKLSLMVHPDKCAHPQAHQAFI 360

Query: 361 RLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEELLA 420
            LN AFKELQDPDKRKALD+KIK KEEQE+FK ELR MREAA+WR+ QGISMEGD+ELLA
Sbjct: 361 ILNKAFKELQDPDKRKALDEKIKLKEEQEEFKAELRAMREAAQWRRLQGISMEGDDELLA 420

Query: 421 NMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSAKEGRGDTSVWTDTPSDRAQKAK 480
            +  EVK  PKRDEWMT+LPPERKPG+TMQS++FSKSAKEGRGDTSVWTDTP +RAQKAK
Sbjct: 421 EV--EVKAPPKRDEWMTTLPPERKPGVTMQSTKFSKSAKEGRGDTSVWTDTPLERAQKAK 480

Query: 481 TSYLEAYNEAAALASNEE--AKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKKSK 540
             YLEAYNEAAALASNEE   K+ SS++ LVDKYN+EKRSKSLV+KH+E A  R KKKSK
Sbjct: 481 MHYLEAYNEAAALASNEEEKTKRSSSDADLVDKYNKEKRSKSLVQKHQEEASKRPKKKSK 540

Query: 541 QVAEKE-EEWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRNFL 595
           Q +EKE +EW GKHPWKPWDREKDLTAGR+S+N D ENM +GL SRFS+GS+QRNFL
Sbjct: 541 QQSEKEKDEWEGKHPWKPWDREKDLTAGRQSVNLDTENMVKGLTSRFSTGSFQRNFL 585

BLAST of Spg017994 vs. TAIR 10
Match: AT1G65280.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 587.0 bits (1512), Expect = 1.7e-167
Identity = 358/600 (59.67%), Postives = 449/600 (74.83%), Query Frame = 0

Query: 10  TDDSEPSSSSLSSSDDSDISSPQLRHRHR------SGSSRRDKDKKRELRIRDRKRKDRN 69
           T  S  SSSS  SSD+SD SS + R + +       GS  R+K +KRE   ++RKRK + 
Sbjct: 15  TPSSATSSSSSYSSDESDSSSRKWRKKQKERRKSDGGSYEREKRRKRE---KERKRK-KI 74

Query: 70  NERDRKRRKKKLKREGRRKRDDYGSESDGESSCSGDSTDNESERRKVKIVEPEQVVRELL 129
             ++RKRR  K K+  + K+ +Y  ESD ES    DS  ++ +       +PE VV+E+L
Sbjct: 75  ERKERKRRDMKKKK--KTKKREY--ESDTESYSGSDSFSDQED-------DPETVVKEML 134

Query: 130 KEFPDVGNDLKQLLKMIDDGQAVDINGISERTLTKRLKKLFLSLNLEESQEKVFLLPSNG 189
            EFP+VGNDLKQLLKMIDDGQAVDI GISE TL KRLKKLFLSL L+E  ++VFLLP   
Sbjct: 135 LEFPNVGNDLKQLLKMIDDGQAVDIKGISESTLKKRLKKLFLSLKLKERGDRVFLLPPGA 194

Query: 190 RPTLEAIGPLIQSHLEQKRNQFDQSGTLDNKSSPPLNVETTQAREDNVSELAYPDSEASG 249
            P+L+ +G LI      K  + D   +LD+ SSP    E   A E+ +   +  D   +G
Sbjct: 195 SPSLDVVGHLI------KGGEEDVEKSLDD-SSPLKETEKGLADENGLGSNSADD--VTG 254

Query: 250 PKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAETANEAE 309
           PK+R++GP MPSAELLAAAAKLTEAQ ELREAELEED+E F+GP PPA+VAE  ++NEAE
Sbjct: 255 PKKRVIGPAMPSAELLAAAAKLTEAQAELREAELEEDSEYFIGPAPPAVVAEVASSNEAE 314

Query: 310 RFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFIRLN 369
           RFEEV+RIM+ + +SPYD+LG+N N++ +N+KKRYWK+SLLVHPDKCSHPQA +AF+ LN
Sbjct: 315 RFEEVTRIMEADANSPYDVLGVNHNMAADNMKKRYWKLSLLVHPDKCSHPQAQEAFVLLN 374

Query: 370 NAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEELLANMD 429
            AFKELQDP+KRKA+DDKIK KEEQE FKVELR M+EAA+WR+SQGISMEGD ELLA   
Sbjct: 375 KAFKELQDPEKRKAMDDKIKLKEEQEAFKVELRSMQEAAQWRRSQGISMEGDAELLA--A 434

Query: 430 DEVKVEPKRDEWMTSLPPERKPGMTM-QSSRFSKSAKEGRGDTSVWTDTPSDRAQKAKTS 489
            EVK EPKRDEWMT+LPPERK G+ + QS+ FS++A+EGRGDT+ WTDTP D+A++AK +
Sbjct: 435 TEVKPEPKRDEWMTTLPPERKVGVAVQQSTTFSRNAREGRGDTTAWTDTPMDKAERAKMN 494

Query: 490 YLEAYNEAAALASNE-EAKKYSSESALVDKYNQEKRSKSLVEKHRE---GAKSRGKKKSK 549
           YLEAYN+A+ALASNE E  K S ++ LVDKYN+EKR+KSLVEKHRE    + SR KKK K
Sbjct: 495 YLEAYNKASALASNEGENMKRSMDAELVDKYNKEKRAKSLVEKHREDSSSSSSRLKKKKK 554

Query: 550 QVAEKE----EEWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRNFL 595
             + KE    +EWVGKHPWKPWDRE DLTAGR+++  D + MA+GL S+FSSG++QR+FL
Sbjct: 555 LSSSKEKTEKDEWVGKHPWKPWDRENDLTAGRQNVKLDADGMAEGLASKFSSGNFQRSFL 588

BLAST of Spg017994 vs. TAIR 10
Match: AT5G22080.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 3.8e-10
Identity = 42/131 (32.06%), Postives = 74/131 (56.49%), Query Frame = 0

Query: 301 EAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFI 360
           E ER  EV RI+     +P++ L L+ + S +++K++Y K+SL+VHPDKC HPQA +AF 
Sbjct: 21  EVERDNEVGRILSCFKLNPFEHLNLSFDSSTDDVKRQYRKISLMVHPDKCKHPQAQEAFG 80

Query: 361 RLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEELLA 420
            L  A + L +  +R  +  ++   +E+ + K + +  ++ A   K + +  EG  E + 
Sbjct: 81  ALAKAQQLLLNDQERDYILTQVHAAKEELKMKRKKQLKKDTAS--KIKSLVDEGKHEHIY 140

Query: 421 NMDDEVKVEPK 432
              +E + E K
Sbjct: 141 EQSEEFQKELK 149

BLAST of Spg017994 vs. TAIR 10
Match: AT5G22080.2 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 3.8e-10
Identity = 42/131 (32.06%), Postives = 74/131 (56.49%), Query Frame = 0

Query: 301 EAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFI 360
           E ER  EV RI+     +P++ L L+ + S +++K++Y K+SL+VHPDKC HPQA +AF 
Sbjct: 21  EVERDNEVGRILSCFKLNPFEHLNLSFDSSTDDVKRQYRKISLMVHPDKCKHPQAQEAFG 80

Query: 361 RLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEELLA 420
            L  A + L +  +R  +  ++   +E+ + K + +  ++ A   K + +  EG  E + 
Sbjct: 81  ALAKAQQLLLNDQERDYILTQVHAAKEELKMKRKKQLKKDTAS--KIKSLVDEGKHEHIY 140

Query: 421 NMDDEVKVEPK 432
              +E + E K
Sbjct: 141 EQSEEFQKELK 149

BLAST of Spg017994 vs. TAIR 10
Match: AT5G49580.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 2.3e-07
Identity = 47/159 (29.56%), Postives = 80/159 (50.31%), Query Frame = 0

Query: 264 KLTEAQTELREAELEEDNEL-----FVGPPPPAMVAEAETANEAERFEEVSRIMDVEGDS 323
           K+ +  T  R  E   D+       F G   P + + + T +E    +E++R+++     
Sbjct: 351 KINQQSTADRPTEPVNDSSFENGPGFPGDRGPGVASTSGTDSELTSEDEIARLLNC--PD 410

Query: 324 PYDILGLNR--NLSIENIKKRYWKMSLLVHPDK-CSHPQANQAFIRLNNAFKELQDPDKR 383
            Y  LGL R  N+ +  +K+ Y K ++LVHPDK   + +A +AF +L NA++ L D  K+
Sbjct: 411 HYSALGLARYGNVDMAYLKREYRKKAMLVHPDKNMGNERAAEAFKKLQNAYEVLLDSVKQ 470

Query: 384 KALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEG 415
           K+ DD++K +E    F+   R    + K  +  G S  G
Sbjct: 471 KSYDDELKREELLNYFR---RFQNSSQKDTRGHGFSGSG 504

BLAST of Spg017994 vs. TAIR 10
Match: AT5G05750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 54.7 bits (130), Expect = 3.0e-07
Identity = 39/143 (27.27%), Postives = 71/143 (49.65%), Query Frame = 0

Query: 245 KRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAM-------VAEAE 304
           K R L P +P   L++   K ++      ++     NE       P++        A   
Sbjct: 33  KARRLDPNLPIDGLVSDLKKQSDEPAAEEDSPGSAANESSKPSDRPSLRQRGSSSSAAGS 92

Query: 305 TANEAERFEEVSRIMDVEGDSP-YDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQAN 364
           +++ +   E+ + + +++     Y+ILGL  N S+E+++K Y K+SL VHPDK   P + 
Sbjct: 93  SSSSSSTEEQRTIVREIKSKKDYYEILGLKSNCSVEDLRKSYRKLSLKVHPDKNKAPGSE 152

Query: 365 QAFIRLNNAFKELQDPDKRKALD 380
           +AF  ++ AF+ L + D R+  D
Sbjct: 153 EAFKSVSKAFQCLSNEDTRRKYD 175