Spg017944 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg017944
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionNAD(P)H dehydrogenase subunit M
Locationscaffold9: 33817748 .. 33819293 (-)
RNA-Seq ExpressionSpg017944
SyntenySpg017944
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGGCAACTAACCAATAAATGCACCAAATCAAACAAAACCAGAAAATTCTTATCCACAAACAATTGGATCTCATTAAAAAAAAAAAAACCAAAAATAAAAAATCCAACCCCTCAAAAAAAAAAGGACTTAAAAACTATAAAACAGAGAAACCAAGCCAAACCAAAACCAACCATTTTCAGAAGGATAGACACCAAAAAAAAAGTTCTTCCATGGCAGCAGCAGCTTCATCTTACATAGTCTTTTCAATGGTGGGTTGGAAAAATGGCAAACAAAACACAAGAACCACAGCCAGATTTCCCATCTCAGCTCAGCTCAATGAAGCTGCAGAGCAACTGAAAGTGGGAGAAGAAGAAGAGGCAAAGCCGAAACCGCCACCGTCACCGTCGCCACCGCCGCTGCGGCCAGTGGAAGCACAGCAGAATGTGAAGAGCAAGAACATGAGCAGAGAGTATGGAGGGCAATGGCTGAGCAGTGTGACTCGCCATGTAAGAATCTATGCAGCTTACATTGACCCTGTTACTTGTGAGTTTGATCAGACCCAAATGGATAAACTCACTCTCATTTTGGACCCAAGCAATGAGTTCCTTTGGAATCCTGAAACATGCAATAAAGTCTATGCTTACTTCCAAGAGCTTGTCGATCATTACGAGGTACTTTTGGCTTCGTTCGATAACCATTTAGTTTTTCACAGTGATTTTCATATTTCTTAGGGAAACATTTGAATTTTTACTCAAATTTCGAAAACAAAAACAAGTTTTTAAAAATTACTTTTTTTAGTTTTCAAAACAAGGAAAATCATGGATTGAGGAAGTATTTATAAACTCAATTTTCGTAAATAAAAAACCGAAAGGTTGTCAAACGAGACCATTTGTTTTTTTCTCTCTCTACTCTTTGATCATTACATTGACCCTGTAACTTGTGGGTTTGAGCTTAGTTTTGATTCATACTCGTAGAATGAAGAAAATGTGCGATAAACATCTTGAATTGCTAATTGCCTGATCTTTCTTTTATGCTCAGGAGAGAACTCGAAGATAGACTTGTTCATATAATCTCATGACGAGAAACGTCGTTTTATTTTTCTATTTTGTTAATGTGCTGTTATTCTAACCAACATTTTGAAAATGTTACTTGTGGAAAACAGGGAGCCCCATTGACAGAATATACACTTCGCCTAATTGGTTCAGACATAGAACACTACATTAGAAAGATGCTTTATGATGGTGAAATCAAGTACAATATGAATGCAAGAGTCCTCAACTTCAGCATGGGAAAGCCAAGAATTATGTTCAATAACAATGATCTGCCTCAGGATCTTCAATCATAGTAAATAGTTTAACAAATGCAAAAATATGTGACATATTTATTGTCTTGTGTTCCAAATAGAATAATAATTCTTCAATTTATACTTACATTTCCTTCCCTGTTTTGTAAACATAGTTTTACGTTAGTTTGTGATTGATTTTGGTCAAGTTTCGACCTAGTCACTATAGTTCGATTGAATTGTTTAAGACATCTCTAGATTGACCAAATGTTAATAAATTTAGG

mRNA sequence

ATGCACCAAATCAAACAAAACCAGAAAATTCTTATCCACAAACAATTGGATCTCATTAAAAAAAAAAAAACCAAAAATAAAAAATCCAACCCCTCAAAAAAAAAAGGACTTAAAAACTATAAAACAGAGAAACCAAGCCAAACCAAAACCAACCATTTTCAGAAGGATAGACACCAAAAAAAAAGTTCTTCCATGGCAGCAGCAGCTTCATCTTACATAGTCTTTTCAATGGTGGGTTGGAAAAATGGCAAACAAAACACAAGAACCACAGCCAGATTTCCCATCTCAGCTCAGCTCAATGAAGCTGCAGAGCAACTGAAAGTGGGAGAAGAAGAAGAGGCAAAGCCGAAACCGCCACCGTCACCGTCGCCACCGCCGCTGCGGCCAGTGGAAGCACAGCAGAATGTGAAGAGCAAGAACATGAGCAGAGAGTATGGAGGGCAATGGCTGAGCAGTGTGACTCGCCATGTAAGAATCTATGCAGCTTACATTGACCCTGTTACTTGTGAGTTTGATCAGACCCAAATGGATAAACTCACTCTCATTTTGGACCCAAGCAATGAGTTCCTTTGGAATCCTGAAACATGCAATAAAGTCTATGCTTACTTCCAAGAGCTTGTCGATCATTACGAGGGAGCCCCATTGACAGAATATACACTTCGCCTAATTGGTTCAGACATAGAACACTACATTAGAAAGATGCTTTATGATGGTGAAATCAAGTACAATATGAATGCAAGAGTCCTCAACTTCAGCATGGGAAAGCCAAGAATTATGTTCAATAACAATGATCTGCCTCAGGATCTTCAATCATAG

Coding sequence (CDS)

ATGCACCAAATCAAACAAAACCAGAAAATTCTTATCCACAAACAATTGGATCTCATTAAAAAAAAAAAAACCAAAAATAAAAAATCCAACCCCTCAAAAAAAAAAGGACTTAAAAACTATAAAACAGAGAAACCAAGCCAAACCAAAACCAACCATTTTCAGAAGGATAGACACCAAAAAAAAAGTTCTTCCATGGCAGCAGCAGCTTCATCTTACATAGTCTTTTCAATGGTGGGTTGGAAAAATGGCAAACAAAACACAAGAACCACAGCCAGATTTCCCATCTCAGCTCAGCTCAATGAAGCTGCAGAGCAACTGAAAGTGGGAGAAGAAGAAGAGGCAAAGCCGAAACCGCCACCGTCACCGTCGCCACCGCCGCTGCGGCCAGTGGAAGCACAGCAGAATGTGAAGAGCAAGAACATGAGCAGAGAGTATGGAGGGCAATGGCTGAGCAGTGTGACTCGCCATGTAAGAATCTATGCAGCTTACATTGACCCTGTTACTTGTGAGTTTGATCAGACCCAAATGGATAAACTCACTCTCATTTTGGACCCAAGCAATGAGTTCCTTTGGAATCCTGAAACATGCAATAAAGTCTATGCTTACTTCCAAGAGCTTGTCGATCATTACGAGGGAGCCCCATTGACAGAATATACACTTCGCCTAATTGGTTCAGACATAGAACACTACATTAGAAAGATGCTTTATGATGGTGAAATCAAGTACAATATGAATGCAAGAGTCCTCAACTTCAGCATGGGAAAGCCAAGAATTATGTTCAATAACAATGATCTGCCTCAGGATCTTCAATCATAG

Protein sequence

MHQIKQNQKILIHKQLDLIKKKKTKNKKSNPSKKKGLKNYKTEKPSQTKTNHFQKDRHQKKSSSMAAAASSYIVFSMVGWKNGKQNTRTTARFPISAQLNEAAEQLKVGEEEEAKPKPPPSPSPPPLRPVEAQQNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILDPSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMNARVLNFSMGKPRIMFNNNDLPQDLQS
Homology
BLAST of Spg017944 vs. NCBI nr
Match: XP_022940621.1 (NAD(P)H-quinone oxidoreductase subunit M, chloroplastic [Cucurbita moschata] >XP_023523685.1 NAD(P)H-quinone oxidoreductase subunit M, chloroplastic [Cucurbita pepo subsp. pepo] >KAG6607714.1 NAD(P)H-quinone oxidoreductase subunit M, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 390.6 bits (1002), Expect = 1.1e-104
Identity = 195/208 (93.75%), Postives = 199/208 (95.67%), Query Frame = 0

Query: 65  MAAAASSYIVFSMVGWKNGKQNTRTTARFPISAQLNEAAEQLKVG-EEEEAKPKPPPSPS 124
           MA AASSY+VFSMVGWKNGKQNTR +ARFPISAQ+NEAAEQLKVG EEEEAKPKPPPS  
Sbjct: 1   MATAASSYMVFSMVGWKNGKQNTRPSARFPISAQINEAAEQLKVGEEEEEAKPKPPPS-- 60

Query: 125 PPPLRPVEAQQNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLIL 184
             PLRPVE Q NVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLIL
Sbjct: 61  --PLRPVETQPNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLIL 120

Query: 185 DPSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYN 244
           DPSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYN
Sbjct: 121 DPSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYN 180

Query: 245 MNARVLNFSMGKPRIMFNNNDLPQDLQS 272
           MNARVLNFSMGKPRIMFN+NDLPQDLQS
Sbjct: 181 MNARVLNFSMGKPRIMFNSNDLPQDLQS 204

BLAST of Spg017944 vs. NCBI nr
Match: XP_022981611.1 (NAD(P)H-quinone oxidoreductase subunit M, chloroplastic [Cucurbita maxima])

HSP 1 Score: 390.2 bits (1001), Expect = 1.5e-104
Identity = 193/207 (93.24%), Postives = 198/207 (95.65%), Query Frame = 0

Query: 65  MAAAASSYIVFSMVGWKNGKQNTRTTARFPISAQLNEAAEQLKVGEEEEAKPKPPPSPSP 124
           M+ AASSY+VFSMVGWKNGKQNTR +ARFPISAQ+NEAAEQLKVGEEEEAKPKPP S   
Sbjct: 1   MSTAASSYMVFSMVGWKNGKQNTRPSARFPISAQINEAAEQLKVGEEEEAKPKPPLS--- 60

Query: 125 PPLRPVEAQQNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILD 184
            PLRPVE Q NVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILD
Sbjct: 61  -PLRPVETQPNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILD 120

Query: 185 PSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNM 244
           PSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNM
Sbjct: 121 PSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNM 180

Query: 245 NARVLNFSMGKPRIMFNNNDLPQDLQS 272
           NARVLNFSMGKPRIMFN+NDLPQDLQS
Sbjct: 181 NARVLNFSMGKPRIMFNSNDLPQDLQS 203

BLAST of Spg017944 vs. NCBI nr
Match: KAG7037286.1 (NAD(P)H-quinone oxidoreductase subunit M, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 384.8 bits (987), Expect = 6.1e-103
Identity = 194/208 (93.27%), Postives = 197/208 (94.71%), Query Frame = 0

Query: 65  MAAAASSYIVFSMVGWKNGKQNTRTTARFPISAQLNEAAEQLKVG-EEEEAKPKPPPSPS 124
           MA AASSY+VFSMVGWKNGKQNTR +ARFPISAQ NEAAEQLKVG EEEEAKPKPP S  
Sbjct: 1   MATAASSYMVFSMVGWKNGKQNTRPSARFPISAQNNEAAEQLKVGEEEEEAKPKPPRS-- 60

Query: 125 PPPLRPVEAQQNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLIL 184
             PLRPVE Q NVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLIL
Sbjct: 61  --PLRPVEMQPNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLIL 120

Query: 185 DPSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYN 244
           DPSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYN
Sbjct: 121 DPSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYN 180

Query: 245 MNARVLNFSMGKPRIMFNNNDLPQDLQS 272
           MNARVLNFSMGKPRIMFN+NDLPQDLQS
Sbjct: 181 MNARVLNFSMGKPRIMFNSNDLPQDLQS 204

BLAST of Spg017944 vs. NCBI nr
Match: XP_008447195.1 (PREDICTED: NAD(P)H-quinone oxidoreductase subunit M, chloroplastic [Cucumis melo] >KAA0041980.1 NAD(P)H-quinone oxidoreductase subunit M [Cucumis melo var. makuwa] >TYK17916.1 NAD(P)H-quinone oxidoreductase subunit M [Cucumis melo var. makuwa])

HSP 1 Score: 374.4 bits (960), Expect = 8.3e-100
Identity = 188/207 (90.82%), Postives = 194/207 (93.72%), Query Frame = 0

Query: 66  AAAASSYIVFSMVGWKNGKQNTRTTARFPISAQLNEAAEQLKVGEEEEAKPKPPPSPSPP 125
           AAAASSYI+FSMVGWKNGKQNT+ T RFPISAQLNEAAEQLKVG++EE + +  P PS  
Sbjct: 4   AAAASSYIMFSMVGWKNGKQNTKHTLRFPISAQLNEAAEQLKVGDQEEDQEQATPKPS-- 63

Query: 126 PLRPVEAQQNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILDP 185
           PLRPVE QQNVKSKNM REYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILDP
Sbjct: 64  PLRPVEVQQNVKSKNMGREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILDP 123

Query: 186 SNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMN 245
           SNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMN
Sbjct: 124 SNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMN 183

Query: 246 ARVLNFSMGKPRIMFNNNDLPQ-DLQS 272
           ARVLNFSMGKPRIMFN NDLPQ DLQS
Sbjct: 184 ARVLNFSMGKPRIMFNANDLPQDDLQS 208

BLAST of Spg017944 vs. NCBI nr
Match: XP_004139803.1 (NAD(P)H-quinone oxidoreductase subunit M, chloroplastic [Cucumis sativus] >KGN44204.1 hypothetical protein Csa_016125 [Cucumis sativus])

HSP 1 Score: 367.9 bits (943), Expect = 7.8e-98
Identity = 183/207 (88.41%), Postives = 192/207 (92.75%), Query Frame = 0

Query: 66  AAAASSYIVFSMVGWKNGKQNTRTTARFPISAQLNEAAEQLKVGEEEEAKPKPPPSPSPP 125
           AAAASSYI+FSMVGWKNG QNT+ T RFPISAQ+NEAAEQLK G++EE + +  P PS  
Sbjct: 4   AAAASSYIMFSMVGWKNGIQNTKHTLRFPISAQINEAAEQLKAGDQEEDQEQAKPKPS-- 63

Query: 126 PLRPVEAQQNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILDP 185
           PLRPVE QQNVKSKNM REYGGQWLSSVTRHVRIYAAYIDP+TCEFDQTQMDKLTL+LDP
Sbjct: 64  PLRPVEVQQNVKSKNMGREYGGQWLSSVTRHVRIYAAYIDPITCEFDQTQMDKLTLLLDP 123

Query: 186 SNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMN 245
           SNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMN
Sbjct: 124 SNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMN 183

Query: 246 ARVLNFSMGKPRIMFNNNDLPQ-DLQS 272
           ARVLNFSMGKPRIMFN NDLPQ DLQS
Sbjct: 184 ARVLNFSMGKPRIMFNANDLPQDDLQS 208

BLAST of Spg017944 vs. ExPASy Swiss-Prot
Match: A7NVJ4 (NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Vitis vinifera OX=29760 GN=ndhM PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.4e-78
Identity = 156/210 (74.29%), Postives = 169/210 (80.48%), Query Frame = 0

Query: 67  AAASSYIV---FSMVGWKNGKQNTRTTARFPISAQLN---EAAEQLKVGEEEEAKPKPPP 126
           AA SSY     FSM+GW  GK+  R    F ISAQ     E ++Q+   EEE+ K K   
Sbjct: 2   AATSSYTACTKFSMLGWIGGKRELRKRRAFFISAQQQAEVEESQQVNAQEEEQEKMKQQG 61

Query: 127 SPSPPPLRPVEAQQNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLT 186
               P  RPVE Q NVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLT
Sbjct: 62  KQKLP--RPVEPQVNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLT 121

Query: 187 LILDPSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEI 246
           LILDP+NEF+W  ETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRK+LYDGEI
Sbjct: 122 LILDPTNEFVWTSETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKLLYDGEI 181

Query: 247 KYNMNARVLNFSMGKPRIMFNNNDLPQDLQ 271
           KYNMNARVLNFSMGKPRI+FN+  LPQ++Q
Sbjct: 182 KYNMNARVLNFSMGKPRILFNDG-LPQNVQ 208

BLAST of Spg017944 vs. ExPASy Swiss-Prot
Match: A9PJQ8 (NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Populus jackii OX=640484 GN=ndhM PE=2 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 2.1e-74
Identity = 145/207 (70.05%), Postives = 162/207 (78.26%), Query Frame = 0

Query: 67  AAASSYIV---FSMVGWKNGKQNTRTTARFPISAQLN-EAAEQLKVGEEEEAKPKPPPSP 126
           AA+SSY+    FSM+GW  G++  +      +S Q   E  E  +V  +EE K K P  P
Sbjct: 2   AASSSYMACAKFSMLGWLGGRRELKMRRVISVSPQEQAEVQESQEVNAQEEEKVKQPVQP 61

Query: 127 SPPPLRPVEAQQNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLI 186
                RPVE Q NVKSKNM REYGGQWLSSVTRHVRIYAAYIDP TCEFDQTQ DKLTLI
Sbjct: 62  -----RPVEPQVNVKSKNMGREYGGQWLSSVTRHVRIYAAYIDPETCEFDQTQTDKLTLI 121

Query: 187 LDPSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKY 246
           LDP++EF+W  ETC KVY+YFQELVDHYEGAPLTEYTLRLIGSDIEHYIRK+LYDGEIKY
Sbjct: 122 LDPTDEFVWTDETCYKVYSYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKLLYDGEIKY 181

Query: 247 NMNARVLNFSMGKPRIMFNNNDLPQDL 270
           NMNARVLNFSMGKPRI+FNN+   QD+
Sbjct: 182 NMNARVLNFSMGKPRILFNNDGQLQDV 203

BLAST of Spg017944 vs. ExPASy Swiss-Prot
Match: Q2V2S7 (NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ndhM PE=2 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 4.9e-63
Identity = 121/168 (72.02%), Postives = 139/168 (82.74%), Query Frame = 0

Query: 101 EAAEQLKVGEEEEAKPKPPP---SPSPPPLRPVEAQQNVKSKNMSREYGGQWLSSVTRHV 160
           E    L+V E E  K +         P PLRPVE Q NVKSK M  ++GGQWLSSVTRHV
Sbjct: 39  EQDSTLEVQETETLKEEQSTEKMKKQPTPLRPVEKQLNVKSKGMG-DFGGQWLSSVTRHV 98

Query: 161 RIYAAYIDPVTCEFDQTQMDKLTLILDPSNEFLWNPETCNKVYAYFQELVDHYEGAPLTE 220
           RIYAAYIDP TCEFDQ+QMDKLTLILDP+ EF+W+ E+CNKVY+YFQELVDHYEGAPLTE
Sbjct: 99  RIYAAYIDPETCEFDQSQMDKLTLILDPTEEFVWDDESCNKVYSYFQELVDHYEGAPLTE 158

Query: 221 YTLRLIGSDIEHYIRKMLYDGEIKYNMNARVLNFSMGKPRIMFNNNDL 266
           YTLRLIGSD+EHYIRKML+DGEI+YNM+ARVLNFSMGKPR+ FN +++
Sbjct: 159 YTLRLIGSDVEHYIRKMLFDGEIQYNMDARVLNFSMGKPRVQFNTSNI 205

BLAST of Spg017944 vs. ExPASy Swiss-Prot
Match: A2XVZ1 (NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=ndhM PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.1e-58
Identity = 116/191 (60.73%), Postives = 139/191 (72.77%), Query Frame = 0

Query: 88  RTTARFPISAQLNEAAEQLKVGEEEEAKPKPPPSPSPP------------PLRPVEAQQN 147
           R + RFP   +  +  +Q+K  EEE A    PP P               P +P+  Q  
Sbjct: 28  RRSVRFP-PVRAQDQQQQVKEEEEEAAVENLPPPPQEEEQRRERKTRRQGPAQPLPVQPL 87

Query: 148 VKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILDPSNEFLWNPET 207
            +SKNMSREYGGQWLS  TRH+RIYAAYI+P T  FDQTQMDKLTL+LDP++EF+W  ET
Sbjct: 88  AESKNMSREYGGQWLSCTTRHIRIYAAYINPETNAFDQTQMDKLTLLLDPTDEFVWTDET 147

Query: 208 CNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMNARVLNFSMGK 267
           C KVY  FQ+LVDHYEGA L+EYTLRLIGSD+EH+IRK+LYDGEIKYNM +RVLNFSMGK
Sbjct: 148 CQKVYDEFQDLVDHYEGAELSEYTLRLIGSDLEHFIRKLLYDGEIKYNMMSRVLNFSMGK 207

BLAST of Spg017944 vs. ExPASy Swiss-Prot
Match: Q7FB12 (NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ndhM PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.1e-58
Identity = 116/191 (60.73%), Postives = 139/191 (72.77%), Query Frame = 0

Query: 88  RTTARFPISAQLNEAAEQLKVGEEEEAKPKPPPSPSPP------------PLRPVEAQQN 147
           R + RFP   +  +  +Q+K  EEE A    PP P               P +P+  Q  
Sbjct: 28  RRSVRFP-PVRAQDQQQQVKEEEEEAAVENLPPPPQEEEQRRERKTRRQGPAQPLPVQPL 87

Query: 148 VKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILDPSNEFLWNPET 207
            +SKNMSREYGGQWLS  TRH+RIYAAYI+P T  FDQTQMDKLTL+LDP++EF+W  ET
Sbjct: 88  AESKNMSREYGGQWLSCTTRHIRIYAAYINPETNAFDQTQMDKLTLLLDPTDEFVWTDET 147

Query: 208 CNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMNARVLNFSMGK 267
           C KVY  FQ+LVDHYEGA L+EYTLRLIGSD+EH+IRK+LYDGEIKYNM +RVLNFSMGK
Sbjct: 148 CQKVYDEFQDLVDHYEGAELSEYTLRLIGSDLEHFIRKLLYDGEIKYNMMSRVLNFSMGK 207

BLAST of Spg017944 vs. ExPASy TrEMBL
Match: A0A6J1FIZ9 (NAD(P)H dehydrogenase subunit M OS=Cucurbita moschata OX=3662 GN=LOC111446160 PE=3 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 5.4e-105
Identity = 195/208 (93.75%), Postives = 199/208 (95.67%), Query Frame = 0

Query: 65  MAAAASSYIVFSMVGWKNGKQNTRTTARFPISAQLNEAAEQLKVG-EEEEAKPKPPPSPS 124
           MA AASSY+VFSMVGWKNGKQNTR +ARFPISAQ+NEAAEQLKVG EEEEAKPKPPPS  
Sbjct: 1   MATAASSYMVFSMVGWKNGKQNTRPSARFPISAQINEAAEQLKVGEEEEEAKPKPPPS-- 60

Query: 125 PPPLRPVEAQQNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLIL 184
             PLRPVE Q NVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLIL
Sbjct: 61  --PLRPVETQPNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLIL 120

Query: 185 DPSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYN 244
           DPSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYN
Sbjct: 121 DPSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYN 180

Query: 245 MNARVLNFSMGKPRIMFNNNDLPQDLQS 272
           MNARVLNFSMGKPRIMFN+NDLPQDLQS
Sbjct: 181 MNARVLNFSMGKPRIMFNSNDLPQDLQS 204

BLAST of Spg017944 vs. ExPASy TrEMBL
Match: A0A6J1IX15 (NAD(P)H dehydrogenase subunit M OS=Cucurbita maxima OX=3661 GN=LOC111480680 PE=3 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 7.1e-105
Identity = 193/207 (93.24%), Postives = 198/207 (95.65%), Query Frame = 0

Query: 65  MAAAASSYIVFSMVGWKNGKQNTRTTARFPISAQLNEAAEQLKVGEEEEAKPKPPPSPSP 124
           M+ AASSY+VFSMVGWKNGKQNTR +ARFPISAQ+NEAAEQLKVGEEEEAKPKPP S   
Sbjct: 1   MSTAASSYMVFSMVGWKNGKQNTRPSARFPISAQINEAAEQLKVGEEEEAKPKPPLS--- 60

Query: 125 PPLRPVEAQQNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILD 184
            PLRPVE Q NVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILD
Sbjct: 61  -PLRPVETQPNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILD 120

Query: 185 PSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNM 244
           PSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNM
Sbjct: 121 PSNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNM 180

Query: 245 NARVLNFSMGKPRIMFNNNDLPQDLQS 272
           NARVLNFSMGKPRIMFN+NDLPQDLQS
Sbjct: 181 NARVLNFSMGKPRIMFNSNDLPQDLQS 203

BLAST of Spg017944 vs. ExPASy TrEMBL
Match: A0A5D3D3P2 (NAD(P)H dehydrogenase subunit M OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G002070 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 4.0e-100
Identity = 188/207 (90.82%), Postives = 194/207 (93.72%), Query Frame = 0

Query: 66  AAAASSYIVFSMVGWKNGKQNTRTTARFPISAQLNEAAEQLKVGEEEEAKPKPPPSPSPP 125
           AAAASSYI+FSMVGWKNGKQNT+ T RFPISAQLNEAAEQLKVG++EE + +  P PS  
Sbjct: 4   AAAASSYIMFSMVGWKNGKQNTKHTLRFPISAQLNEAAEQLKVGDQEEDQEQATPKPS-- 63

Query: 126 PLRPVEAQQNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILDP 185
           PLRPVE QQNVKSKNM REYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILDP
Sbjct: 64  PLRPVEVQQNVKSKNMGREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILDP 123

Query: 186 SNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMN 245
           SNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMN
Sbjct: 124 SNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMN 183

Query: 246 ARVLNFSMGKPRIMFNNNDLPQ-DLQS 272
           ARVLNFSMGKPRIMFN NDLPQ DLQS
Sbjct: 184 ARVLNFSMGKPRIMFNANDLPQDDLQS 208

BLAST of Spg017944 vs. ExPASy TrEMBL
Match: A0A1S3BGV5 (NAD(P)H dehydrogenase subunit M OS=Cucumis melo OX=3656 GN=LOC103489705 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 4.0e-100
Identity = 188/207 (90.82%), Postives = 194/207 (93.72%), Query Frame = 0

Query: 66  AAAASSYIVFSMVGWKNGKQNTRTTARFPISAQLNEAAEQLKVGEEEEAKPKPPPSPSPP 125
           AAAASSYI+FSMVGWKNGKQNT+ T RFPISAQLNEAAEQLKVG++EE + +  P PS  
Sbjct: 4   AAAASSYIMFSMVGWKNGKQNTKHTLRFPISAQLNEAAEQLKVGDQEEDQEQATPKPS-- 63

Query: 126 PLRPVEAQQNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILDP 185
           PLRPVE QQNVKSKNM REYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILDP
Sbjct: 64  PLRPVEVQQNVKSKNMGREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILDP 123

Query: 186 SNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMN 245
           SNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMN
Sbjct: 124 SNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMN 183

Query: 246 ARVLNFSMGKPRIMFNNNDLPQ-DLQS 272
           ARVLNFSMGKPRIMFN NDLPQ DLQS
Sbjct: 184 ARVLNFSMGKPRIMFNANDLPQDDLQS 208

BLAST of Spg017944 vs. ExPASy TrEMBL
Match: A0A0A0K5H1 (NAD(P)H dehydrogenase subunit M OS=Cucumis sativus OX=3659 GN=Csa_7G221360 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.8e-98
Identity = 183/207 (88.41%), Postives = 192/207 (92.75%), Query Frame = 0

Query: 66  AAAASSYIVFSMVGWKNGKQNTRTTARFPISAQLNEAAEQLKVGEEEEAKPKPPPSPSPP 125
           AAAASSYI+FSMVGWKNG QNT+ T RFPISAQ+NEAAEQLK G++EE + +  P PS  
Sbjct: 4   AAAASSYIMFSMVGWKNGIQNTKHTLRFPISAQINEAAEQLKAGDQEEDQEQAKPKPS-- 63

Query: 126 PLRPVEAQQNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILDP 185
           PLRPVE QQNVKSKNM REYGGQWLSSVTRHVRIYAAYIDP+TCEFDQTQMDKLTL+LDP
Sbjct: 64  PLRPVEVQQNVKSKNMGREYGGQWLSSVTRHVRIYAAYIDPITCEFDQTQMDKLTLLLDP 123

Query: 186 SNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMN 245
           SNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMN
Sbjct: 124 SNEFLWNPETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKMLYDGEIKYNMN 183

Query: 246 ARVLNFSMGKPRIMFNNNDLPQ-DLQS 272
           ARVLNFSMGKPRIMFN NDLPQ DLQS
Sbjct: 184 ARVLNFSMGKPRIMFNANDLPQDDLQS 208

BLAST of Spg017944 vs. TAIR 10
Match: AT4G37925.1 (subunit NDH-M of NAD(P)H:plastoquinone dehydrogenase complex )

HSP 1 Score: 242.7 bits (618), Expect = 3.5e-64
Identity = 121/168 (72.02%), Postives = 139/168 (82.74%), Query Frame = 0

Query: 101 EAAEQLKVGEEEEAKPKPPP---SPSPPPLRPVEAQQNVKSKNMSREYGGQWLSSVTRHV 160
           E    L+V E E  K +         P PLRPVE Q NVKSK M  ++GGQWLSSVTRHV
Sbjct: 39  EQDSTLEVQETETLKEEQSTEKMKKQPTPLRPVEKQLNVKSKGMG-DFGGQWLSSVTRHV 98

Query: 161 RIYAAYIDPVTCEFDQTQMDKLTLILDPSNEFLWNPETCNKVYAYFQELVDHYEGAPLTE 220
           RIYAAYIDP TCEFDQ+QMDKLTLILDP+ EF+W+ E+CNKVY+YFQELVDHYEGAPLTE
Sbjct: 99  RIYAAYIDPETCEFDQSQMDKLTLILDPTEEFVWDDESCNKVYSYFQELVDHYEGAPLTE 158

Query: 221 YTLRLIGSDIEHYIRKMLYDGEIKYNMNARVLNFSMGKPRIMFNNNDL 266
           YTLRLIGSD+EHYIRKML+DGEI+YNM+ARVLNFSMGKPR+ FN +++
Sbjct: 159 YTLRLIGSDVEHYIRKMLFDGEIQYNMDARVLNFSMGKPRVQFNTSNI 205

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022940621.11.1e-10493.75NAD(P)H-quinone oxidoreductase subunit M, chloroplastic [Cucurbita moschata] >XP... [more]
XP_022981611.11.5e-10493.24NAD(P)H-quinone oxidoreductase subunit M, chloroplastic [Cucurbita maxima][more]
KAG7037286.16.1e-10393.27NAD(P)H-quinone oxidoreductase subunit M, chloroplastic, partial [Cucurbita argy... [more]
XP_008447195.18.3e-10090.82PREDICTED: NAD(P)H-quinone oxidoreductase subunit M, chloroplastic [Cucumis melo... [more]
XP_004139803.17.8e-9888.41NAD(P)H-quinone oxidoreductase subunit M, chloroplastic [Cucumis sativus] >KGN44... [more]
Match NameE-valueIdentityDescription
A7NVJ41.4e-7874.29NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Vitis vinifera OX=297... [more]
A9PJQ82.1e-7470.05NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Populus jackii OX=640... [more]
Q2V2S74.9e-6372.02NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Arabidopsis thaliana ... [more]
A2XVZ12.1e-5860.73NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Oryza sativa subsp. i... [more]
Q7FB122.1e-5860.73NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Oryza sativa subsp. j... [more]
Match NameE-valueIdentityDescription
A0A6J1FIZ95.4e-10593.75NAD(P)H dehydrogenase subunit M OS=Cucurbita moschata OX=3662 GN=LOC111446160 PE... [more]
A0A6J1IX157.1e-10593.24NAD(P)H dehydrogenase subunit M OS=Cucurbita maxima OX=3661 GN=LOC111480680 PE=3... [more]
A0A5D3D3P24.0e-10090.82NAD(P)H dehydrogenase subunit M OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3BGV54.0e-10090.82NAD(P)H dehydrogenase subunit M OS=Cucumis melo OX=3656 GN=LOC103489705 PE=3 SV=... [more]
A0A0A0K5H13.8e-9888.41NAD(P)H dehydrogenase subunit M OS=Cucumis sativus OX=3659 GN=Csa_7G221360 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT4G37925.13.5e-6472.02subunit NDH-M of NAD(P)H:plastoquinone dehydrogenase complex [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018922NAD(P)H-quinone oxidoreductase subunit MPFAMPF10664NdhMcoord: 150..258
e-value: 2.2E-45
score: 153.4
IPR018922NAD(P)H-quinone oxidoreductase subunit MPANTHERPTHR36900NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT M, CHLOROPLASTICcoord: 66..268
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..65
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 108..138
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..35
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 48..62

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg017944.1Spg017944.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009535 chloroplast thylakoid membrane
molecular_function GO:0016655 oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
molecular_function GO:0048038 quinone binding