Spg017848 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg017848
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionplant-specific TFIIB-related protein PTF2
Locationscaffold9: 35517298 .. 35518875 (-)
RNA-Seq ExpressionSpg017848
SyntenySpg017848
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGGCTCCTGCAATAATTGCCACAGCAGGTCCCTCTTCCGCGACGATGTTTCCGGCAACCTGATTTGCTCCTCCTGCGGCATTGTTCAGGAATTTGACAATTATGAAGCCCAGCTTTGCGGTGTCAATGGTCCACAGGGCACATTCATTCGCGTTGGAACCTCAGGTTCGGGCAGTGTTTTGATTTATAAGGATAAGAAAATCTTCGAGGCTCAAAAAGTTATTGAAGATATTACGTTTAGATTGGGATTTTCTGCTTCTAAATCTAACGATGTTAGGATTTTAGTATCGACGATTACGGAGGGTGAGTATGGGTTAGGCGATTGGTTCACAATTCTCGTTGGTGCTTGTGCTTATGTTTCAATGCGTAAGGGTAATCGTCCTTTACCAATGTCAGAGGTTGCATCGACCATCGAATGTGATCTTCACGAACTTGGCAGAATGGTTCTCAGAGTGGTTGATTTTTTGGATTTGAAAGGGTCTGAATTTCCGGTGTTTGATATTGTGGGGTCATTAGAGCGGGCGGCAAGGAATTCACCGTCTTTCTCAAAAGTAGAAGCAGATAGGATGGAGAGAATAGTTAAGCAAGGAATCTTTTTGTTACAGTGTGCTATGAAATGGTGTCTGACGACTGGGCGGCAACCACTACCCATGGTGGCTGCCGTGTTGGTTCTGGTGTCAAAGTTGAATGAGGTTGATGTCAGTATTGACAATGTAGCAACGGAGGTTCATGCCAATGTTTCTACAAGTAAGAAAAGGTACAGGGAACTTTTGGAGGCACTTGTGGAAGTTGGCAAAAAGTTGCCTTGGGGAAAAGATATCACAATAAAGAATATTGTGAAGAATGCTCCTTTTGTGATTCAGTATATGGAGTTGAAATCAATGTCAAATGCCAGTGCTAAGGGGAAGAATTTGGAGAACGTGGGGATTGATTTGCAGGTCGCAGTAAATGATTGTTTAAGAAAAGAGCTAGAGTATGAGAGTGAAGTTAATAATTTTGAAGACGATTCTCAGTATTTTGAGTCACGAGACAATAGTTGGGTTGATGAACCGAGTTGTGACGATGGGAACAAGCTCAATATTTCACATGAATGTTTGTCCTTGATTTATAATAAGTTTTTGAATGAAATGGCTCATCTCAGGTCATCGGGTGGAATTGCAGAGGTCTATGGAAGAAAGCAGGGAAGAAAAGTTGCTTTTCACTCTTGTACAGAGTGGTGGGAGGGAAAATCAGAATTAAGCAAAAAGCTTATGTTACAACAATTATTGGAGACTGATGTAGGATTGAATGGTGTCCCACCGTCATTTGTCTCTAGTTGCAATGCATACAAAAGGAGGAAGGAAAAAATAAATGCTGCTAAGAAGCGAATTCAAAGGATCATGTTTCCTTCCAATGTTGTGACTAGTGATGTAAATGTTGAGAAGAAAAGAAAGCGTAAGGGAGCTAATGGCATAGAATGGGAAGATATAATAATCGAAACCCTTCTTCTTCATGGCGTGAAAGAGGAGGAAATTGAGAAGGGTCATTACAATGTCTTGCTGGATTTGCATGTTTTCAGTTCTTGGAATTCATAG

mRNA sequence

ATGTCTGGCTCCTGCAATAATTGCCACAGCAGGTCCCTCTTCCGCGACGATGTTTCCGGCAACCTGATTTGCTCCTCCTGCGGCATTGTTCAGGAATTTGACAATTATGAAGCCCAGCTTTGCGGTGTCAATGGTCCACAGGGCACATTCATTCGCGTTGGAACCTCAGGTTCGGGCAGTGTTTTGATTTATAAGGATAAGAAAATCTTCGAGGCTCAAAAAGTTATTGAAGATATTACGTTTAGATTGGGATTTTCTGCTTCTAAATCTAACGATGTTAGGATTTTAGTATCGACGATTACGGAGGGTGAGTATGGGTTAGGCGATTGGTTCACAATTCTCGTTGGTGCTTGTGCTTATGTTTCAATGCGTAAGGGTAATCGTCCTTTACCAATGTCAGAGGTTGCATCGACCATCGAATGTGATCTTCACGAACTTGGCAGAATGGTTCTCAGAGTGGTTGATTTTTTGGATTTGAAAGGGTCTGAATTTCCGGTGTTTGATATTGTGGGGTCATTAGAGCGGGCGGCAAGGAATTCACCGTCTTTCTCAAAAGTAGAAGCAGATAGGATGGAGAGAATAGTTAAGCAAGGAATCTTTTTGTTACAGTGTGCTATGAAATGGTGTCTGACGACTGGGCGGCAACCACTACCCATGGTGGCTGCCGTGTTGGTTCTGGTGTCAAAGTTGAATGAGGTTGATGTCAGTATTGACAATGTAGCAACGGAGGTTCATGCCAATGTTTCTACAAGTAAGAAAAGGTACAGGGAACTTTTGGAGGCACTTGTGGAAGTTGGCAAAAAGTTGCCTTGGGGAAAAGATATCACAATAAAGAATATTGTGAAGAATGCTCCTTTTGTGATTCAGTATATGGAGTTGAAATCAATGTCAAATGCCAGTGCTAAGGGGAAGAATTTGGAGAACGTGGGGATTGATTTGCAGGTCGCAGTAAATGATTGTTTAAGAAAAGAGCTAGAGTATGAGAGTGAAGTTAATAATTTTGAAGACGATTCTCAGTATTTTGAGTCACGAGACAATAGTTGGGTTGATGAACCGAGTTGTGACGATGGGAACAAGCTCAATATTTCACATGAATGTTTGTCCTTGATTTATAATAAGTTTTTGAATGAAATGGCTCATCTCAGGTCATCGGGTGGAATTGCAGAGGTCTATGGAAGAAAGCAGGGAAGAAAAGTTGCTTTTCACTCTTGTACAGAGTGGTGGGAGGGAAAATCAGAATTAAGCAAAAAGCTTATGTTACAACAATTATTGGAGACTGATGTAGGATTGAATGGTGTCCCACCGTCATTTGTCTCTAGTTGCAATGCATACAAAAGGAGGAAGGAAAAAATAAATGCTGCTAAGAAGCGAATTCAAAGGATCATGTTTCCTTCCAATGTTGTGACTAGTGATGTAAATGTTGAGAAGAAAAGAAAGCGTAAGGGAGCTAATGGCATAGAATGGGAAGATATAATAATCGAAACCCTTCTTCTTCATGGCGTGAAAGAGGAGGAAATTGAGAAGGGTCATTACAATGTCTTGCTGGATTTGCATGTTTTCAGTTCTTGGAATTCATAG

Coding sequence (CDS)

ATGTCTGGCTCCTGCAATAATTGCCACAGCAGGTCCCTCTTCCGCGACGATGTTTCCGGCAACCTGATTTGCTCCTCCTGCGGCATTGTTCAGGAATTTGACAATTATGAAGCCCAGCTTTGCGGTGTCAATGGTCCACAGGGCACATTCATTCGCGTTGGAACCTCAGGTTCGGGCAGTGTTTTGATTTATAAGGATAAGAAAATCTTCGAGGCTCAAAAAGTTATTGAAGATATTACGTTTAGATTGGGATTTTCTGCTTCTAAATCTAACGATGTTAGGATTTTAGTATCGACGATTACGGAGGGTGAGTATGGGTTAGGCGATTGGTTCACAATTCTCGTTGGTGCTTGTGCTTATGTTTCAATGCGTAAGGGTAATCGTCCTTTACCAATGTCAGAGGTTGCATCGACCATCGAATGTGATCTTCACGAACTTGGCAGAATGGTTCTCAGAGTGGTTGATTTTTTGGATTTGAAAGGGTCTGAATTTCCGGTGTTTGATATTGTGGGGTCATTAGAGCGGGCGGCAAGGAATTCACCGTCTTTCTCAAAAGTAGAAGCAGATAGGATGGAGAGAATAGTTAAGCAAGGAATCTTTTTGTTACAGTGTGCTATGAAATGGTGTCTGACGACTGGGCGGCAACCACTACCCATGGTGGCTGCCGTGTTGGTTCTGGTGTCAAAGTTGAATGAGGTTGATGTCAGTATTGACAATGTAGCAACGGAGGTTCATGCCAATGTTTCTACAAGTAAGAAAAGGTACAGGGAACTTTTGGAGGCACTTGTGGAAGTTGGCAAAAAGTTGCCTTGGGGAAAAGATATCACAATAAAGAATATTGTGAAGAATGCTCCTTTTGTGATTCAGTATATGGAGTTGAAATCAATGTCAAATGCCAGTGCTAAGGGGAAGAATTTGGAGAACGTGGGGATTGATTTGCAGGTCGCAGTAAATGATTGTTTAAGAAAAGAGCTAGAGTATGAGAGTGAAGTTAATAATTTTGAAGACGATTCTCAGTATTTTGAGTCACGAGACAATAGTTGGGTTGATGAACCGAGTTGTGACGATGGGAACAAGCTCAATATTTCACATGAATGTTTGTCCTTGATTTATAATAAGTTTTTGAATGAAATGGCTCATCTCAGGTCATCGGGTGGAATTGCAGAGGTCTATGGAAGAAAGCAGGGAAGAAAAGTTGCTTTTCACTCTTGTACAGAGTGGTGGGAGGGAAAATCAGAATTAAGCAAAAAGCTTATGTTACAACAATTATTGGAGACTGATGTAGGATTGAATGGTGTCCCACCGTCATTTGTCTCTAGTTGCAATGCATACAAAAGGAGGAAGGAAAAAATAAATGCTGCTAAGAAGCGAATTCAAAGGATCATGTTTCCTTCCAATGTTGTGACTAGTGATGTAAATGTTGAGAAGAAAAGAAAGCGTAAGGGAGCTAATGGCATAGAATGGGAAGATATAATAATCGAAACCCTTCTTCTTCATGGCGTGAAAGAGGAGGAAATTGAGAAGGGTCATTACAATGTCTTGCTGGATTTGCATGTTTTCAGTTCTTGGAATTCATAG

Protein sequence

MSGSCNNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGVNGPQGTFIRVGTSGSGSVLIYKDKKIFEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFTILVGACAYVSMRKGNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGSEFPVFDIVGSLERAARNSPSFSKVEADRMERIVKQGIFLLQCAMKWCLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNVATEVHANVSTSKKRYRELLEALVEVGKKLPWGKDITIKNIVKNAPFVIQYMELKSMSNASAKGKNLENVGIDLQVAVNDCLRKELEYESEVNNFEDDSQYFESRDNSWVDEPSCDDGNKLNISHECLSLIYNKFLNEMAHLRSSGGIAEVYGRKQGRKVAFHSCTEWWEGKSELSKKLMLQQLLETDVGLNGVPPSFVSSCNAYKRRKEKINAAKKRIQRIMFPSNVVTSDVNVEKKRKRKGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFSSWNS
Homology
BLAST of Spg017848 vs. NCBI nr
Match: XP_038893651.1 (plant-specific TFIIB-related protein PTF2 [Benincasa hispida])

HSP 1 Score: 922.9 bits (2384), Expect = 1.2e-264
Identity = 458/521 (87.91%), Postives = 484/521 (92.90%), Query Frame = 0

Query: 1   MSGSCNNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGVNGPQGTFIRVGTSGSGS 60
           MSGSC NCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQL G+NGP GTF+RVGTSGSGS
Sbjct: 1   MSGSCKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLSGINGPLGTFVRVGTSGSGS 60

Query: 61  VLIYKDKKIFEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFTILVGACAY 120
           VL YKDKKIFEAQK+IEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWF ILVGACAY
Sbjct: 61  VLNYKDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKGNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGSEFPVFDIVGSLERAARNS 180
           VSMRK NRPLPMSEVAS +ECDLHELGRMV+RVVDFLDLKG EFPVFDIVGSLERA RNS
Sbjct: 121 VSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERATRNS 180

Query: 181 PSFSKVEADRMERIVKQGIFLLQCAMKWCLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNV 240
           PSFS+V+AD +ERIVKQGIFLLQCAMKW LTTGRQPLPMVAAVLVLVSKLNEVDVSIDNV
Sbjct: 181 PSFSRVDADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNV 240

Query: 241 ATEVHANVSTSKKRYRELLEALVEVGKKLPWGKDITIKNIVKNAPFVIQYMELKSMSNAS 300
           A EVHA VST KKRYRELLEALV+VGKKLPWGKDIT KNIVKNAPFVIQYMELKSMSNAS
Sbjct: 241 AMEVHAKVSTCKKRYRELLEALVKVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300

Query: 301 AKGKNLENVGIDLQVAVNDCLRKELEYESEVNNFEDDSQYFESRDNSWVDEPSCDDGNKL 360
            KGK+LENVG+DLQ AV++CLRKELEYESEVNN EDDSQYFE + +SW DE SCD+GN+L
Sbjct: 301 GKGKDLENVGVDLQSAVSECLRKELEYESEVNNLEDDSQYFELQGSSWRDESSCDNGNRL 360

Query: 361 NISHECLSLIYNKFLNEMAHLRSSGGIAEVYGRKQGRKVAFHSCTEWWEGKSELSKKLML 420
           NISHECLSLIY+KFL+EMAHL+SSGGI EVYGRKQGRK  F+S TEWWEGKSELSKKL+L
Sbjct: 361 NISHECLSLIYHKFLDEMAHLKSSGGINEVYGRKQGRKAGFYSSTEWWEGKSELSKKLLL 420

Query: 421 QQLLETDVGLNGVPPSFVSSCNAYKRRKEKINAAKKRIQRIMFPSNVVTSDVNVEKKRKR 480
           QQLLE D+G  G+PPSFVSSCNAYKRRKEKI AAKKRIQRIM PSN    DVN+EKKRKR
Sbjct: 421 QQLLEIDIGSQGIPPSFVSSCNAYKRRKEKIIAAKKRIQRIMLPSNAPIGDVNIEKKRKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFS 522
           KG NGIEWEDIIIETLLLHGVKEEEIEKGHYN LLDLHVF+
Sbjct: 481 KGTNGIEWEDIIIETLLLHGVKEEEIEKGHYNALLDLHVFN 521

BLAST of Spg017848 vs. NCBI nr
Match: XP_008443157.1 (PREDICTED: transcription factor IIIB 50 kDa subunit [Cucumis melo] >KAA0057033.1 transcription factor IIIB 50 kDa subunit [Cucumis melo var. makuwa])

HSP 1 Score: 908.3 bits (2346), Expect = 3.1e-260
Identity = 450/522 (86.21%), Postives = 486/522 (93.10%), Query Frame = 0

Query: 1   MSGSCNNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGVNGPQGTFIRVGTSGSGS 60
           MSGSC +C+SRS+FRDDVSGNLICSSCGIVQEF+NYEAQL G+NGPQGTF+RVGTSGSGS
Sbjct: 1   MSGSCKDCNSRSIFRDDVSGNLICSSCGIVQEFENYEAQLGGINGPQGTFVRVGTSGSGS 60

Query: 61  VLIYKDKKIFEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFTILVGACAY 120
           VL YKDKK++EAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWF ILVGACAY
Sbjct: 61  VLNYKDKKVYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKGNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGSEFPVFDIVGSLERAARNS 180
           VSMRK NRPL MSEVAS +ECDLHELGRMV+RVVDFLDL+GSEFPVFDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLSMSEVASAVECDLHELGRMVMRVVDFLDLRGSEFPVFDIVGSLERAARNS 180

Query: 181 PSFSKVEADRMERIVKQGIFLLQCAMKWCLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNV 240
           PSFS++EAD +ERIVKQGIFLLQCAMKW LTTGRQPLPMVAAVLVLVSKLNEVDVS++NV
Sbjct: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSMENV 240

Query: 241 ATEVHANVSTSKKRYRELLEALVEVGKKLPWGKDITIKNIVKNAPFVIQYMELKSMSNAS 300
           A EVHA VST KKRYRELLEALVEVGKKLPWGKDIT KNIVKNAPFVIQYMELKSMSNAS
Sbjct: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300

Query: 301 AKGKNLENVGIDLQVAVNDCLRKELEYESEVNNFEDDSQYFESRDNSWVDEPSCDDGNKL 360
            KGK+LENVGIDLQ AV++CLRKELEYESEVNN EDDSQYFE + + W DE +CD+GN+L
Sbjct: 301 GKGKDLENVGIDLQSAVSECLRKELEYESEVNNLEDDSQYFELQGSRWCDESNCDNGNRL 360

Query: 361 NISHECLSLIYNKFLNEMAHLRSSGGIAEVYGRKQGRKVAFHSCTEWWEGKSELSKKLML 420
           NISHECLSLIYNKFL+EMA LRSSGGI +VYGRKQGRK  F+S TEWWEGKS LSKKL+L
Sbjct: 361 NISHECLSLIYNKFLDEMAELRSSGGINDVYGRKQGRKAGFYSSTEWWEGKSVLSKKLLL 420

Query: 421 QQLLETDVGLNGVPPSFVSSCNAYKRRKEKINAAKKRIQRIMFPSNVVTSDVNVEKKRKR 480
           QQLLETD+G  G+PPSFVSSCNAYKRRKEK+NAAKKRIQRIM PSN    DVN++KKRKR
Sbjct: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIQRIMHPSNAPAGDVNIKKKRKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFSS 523
           KGA+ IEWEDIIIETLLLHGV+EEEIEKGHY VLLDLHVF+S
Sbjct: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLHVFTS 522

BLAST of Spg017848 vs. NCBI nr
Match: XP_023542889.1 (plant-specific TFIIB-related protein PTF2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 895.6 bits (2313), Expect = 2.1e-256
Identity = 449/525 (85.52%), Postives = 473/525 (90.10%), Query Frame = 0

Query: 1   MSGSCNNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGVNGPQGTFIRVGTSGSGS 60
           MSGSC NC  RSLFRDDVSGNLICSSCGIVQEFDNY+AQL G+NGPQGTF+RVGTSGSGS
Sbjct: 1   MSGSCKNCSGRSLFRDDVSGNLICSSCGIVQEFDNYDAQLGGLNGPQGTFVRVGTSGSGS 60

Query: 61  VLIYKDKKIFEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFTILVGACAY 120
           VL YKDKKI+EAQK+IEDITFRLGFSASKSNDVRILVSTITEGEYG GDWF+ILVGAC+Y
Sbjct: 61  VLNYKDKKIYEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGQGDWFSILVGACSY 120

Query: 121 VSMRKGNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGSEFPVFDIVGSLERAARNS 180
           VSMRK NRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKG  FP FDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGPAFPGFDIVGSLERAARNS 180

Query: 181 PSFSKVEADRMERIVKQGIFLLQCAMKWCLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNV 240
           PSFSKVEAD ++ IVKQGIFLLQCAMKW LTTGRQPLPMVAAVLVLVS LNEV+VSID+V
Sbjct: 181 PSFSKVEADTLDIIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSGLNEVNVSIDDV 240

Query: 241 ATEVHANVSTSKKRYRELLEALVEVGKKLPWGKDITIKNIVKNAPFVIQYMELKSMSNAS 300
           A EVHA VST KKRYRELL ALVEVGKKLPWGKDIT +NIVKNAPFVIQYMELKSMSN  
Sbjct: 241 AMEVHAKVSTCKKRYRELLGALVEVGKKLPWGKDITKRNIVKNAPFVIQYMELKSMSNTC 300

Query: 301 AKGKNLENVGIDLQVAVNDCLRKELEYESEVNNFEDDSQYFESRDNSWVDEPSCDDGNKL 360
            +GKN E+VGIDLQ AV++CLRKEL+YESEVNN EDDSQYFE   NSW DE SCDD NKL
Sbjct: 301 GRGKNFEDVGIDLQAAVSECLRKELQYESEVNNLEDDSQYFELIGNSWRDESSCDDRNKL 360

Query: 361 NISHECLSLIYNKFLNEMAHLRSSGGIAEVYGRKQGRKVAFHSCTEWWEGKSELSKKLML 420
           NISHECLSLIY KFL+EMAHLRSSGGI E+YGRK GRK  F S TEWWEGKS+LSKKL+L
Sbjct: 361 NISHECLSLIYTKFLDEMAHLRSSGGINEIYGRKLGRKAGFQSSTEWWEGKSQLSKKLLL 420

Query: 421 QQLLETDVGLNGVPPSFVSSCNAYKRRKEKINAAKKRIQRIMFPSNVVTSDVNVEKKRKR 480
            QLLETDVGLNG+PPSFV+SCNAYKRRKEKINAAK RIQRIM PSN    DVN EK+RKR
Sbjct: 421 HQLLETDVGLNGMPPSFVTSCNAYKRRKEKINAAKNRIQRIMLPSNAAIGDVNAEKERKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFSSWNS 526
           K A GIEWEDIIIETLLLH VKEEEIEKGHYN LLDLHVF SW S
Sbjct: 481 KRAIGIEWEDIIIETLLLHDVKEEEIEKGHYNALLDLHVFGSWRS 525

BLAST of Spg017848 vs. NCBI nr
Match: KAG6573303.1 (Plant-specific TFIIB-related protein PTF2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 894.4 bits (2310), Expect = 4.6e-256
Identity = 448/525 (85.33%), Postives = 474/525 (90.29%), Query Frame = 0

Query: 1   MSGSCNNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGVNGPQGTFIRVGTSGSGS 60
           MSGSC NC  RSLFRDDVSGNLICSSCGIVQEFDNY+AQL G+NGPQGTF+RVGTSGSGS
Sbjct: 1   MSGSCKNCSGRSLFRDDVSGNLICSSCGIVQEFDNYDAQLGGLNGPQGTFVRVGTSGSGS 60

Query: 61  VLIYKDKKIFEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFTILVGACAY 120
           VL YKDKKI+EAQK+IEDITFRLGFSASKSNDVRILVSTITEGEYG GDWF+ILVGAC+Y
Sbjct: 61  VLNYKDKKIYEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGQGDWFSILVGACSY 120

Query: 121 VSMRKGNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGSEFPVFDIVGSLERAARNS 180
           VSMRK NRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKG  FP FDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGPAFPGFDIVGSLERAARNS 180

Query: 181 PSFSKVEADRMERIVKQGIFLLQCAMKWCLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNV 240
           PSFSKVEAD ++ IVKQGIFLLQCAMKW LTTGRQPLPMVAAVLVLVS LNEV+VSID+V
Sbjct: 181 PSFSKVEADTLDVIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSGLNEVNVSIDDV 240

Query: 241 ATEVHANVSTSKKRYRELLEALVEVGKKLPWGKDITIKNIVKNAPFVIQYMELKSMSNAS 300
           A EVHA VST KKRYRELL ALVEVGKKLPWGKDIT +NIVKNAPFVIQYMELKSMSN  
Sbjct: 241 AMEVHAKVSTCKKRYRELLGALVEVGKKLPWGKDITKRNIVKNAPFVIQYMELKSMSNTC 300

Query: 301 AKGKNLENVGIDLQVAVNDCLRKELEYESEVNNFEDDSQYFESRDNSWVDEPSCDDGNKL 360
            +GKN E+VGIDLQ AV++CLRKEL+YESEVNN EDDSQYFE   NSW DE SCDD NKL
Sbjct: 301 GQGKNFEDVGIDLQAAVSECLRKELQYESEVNNLEDDSQYFELIGNSWRDESSCDDRNKL 360

Query: 361 NISHECLSLIYNKFLNEMAHLRSSGGIAEVYGRKQGRKVAFHSCTEWWEGKSELSKKLML 420
           NISHECLSLIY KFL+EMAHLRSSGGI E+YGRK GRK  F S TEWWEGKS+LSKKL+L
Sbjct: 361 NISHECLSLIYTKFLDEMAHLRSSGGINEIYGRKLGRKAGFQSSTEWWEGKSQLSKKLLL 420

Query: 421 QQLLETDVGLNGVPPSFVSSCNAYKRRKEKINAAKKRIQRIMFPSNVVTSDVNVEKKRKR 480
            QLLETD+GLNG+PPSFV+SCNAYKRRKEKINAAK RIQRIM PS+    DVN EK+RKR
Sbjct: 421 HQLLETDIGLNGMPPSFVTSCNAYKRRKEKINAAKNRIQRIMLPSHAAIGDVNAEKERKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFSSWNS 526
           K A GIEWEDIIIETLLLH VKEEEIEKGHYN LLDLHVFSSW S
Sbjct: 481 KRAIGIEWEDIIIETLLLHDVKEEEIEKGHYNALLDLHVFSSWRS 525

BLAST of Spg017848 vs. NCBI nr
Match: XP_022955013.1 (plant-specific TFIIB-related protein PTF2 [Cucurbita moschata])

HSP 1 Score: 893.3 bits (2307), Expect = 1.0e-255
Identity = 448/525 (85.33%), Postives = 473/525 (90.10%), Query Frame = 0

Query: 1   MSGSCNNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGVNGPQGTFIRVGTSGSGS 60
           MSGSC NC  RSLFRDDVSGNLICSSCGIVQEFDNY+AQL G+NGPQGTF+RVGTSGSGS
Sbjct: 1   MSGSCKNCSGRSLFRDDVSGNLICSSCGIVQEFDNYDAQLGGLNGPQGTFVRVGTSGSGS 60

Query: 61  VLIYKDKKIFEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFTILVGACAY 120
           VL YKDKKI+EAQ +IEDITFRLGFSASKSNDVRILVSTITEGEYG GDWF+ILVGAC+Y
Sbjct: 61  VLNYKDKKIYEAQNIIEDITFRLGFSASKSNDVRILVSTITEGEYGQGDWFSILVGACSY 120

Query: 121 VSMRKGNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGSEFPVFDIVGSLERAARNS 180
           VSMRK NRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKG  FP FDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGPAFPGFDIVGSLERAARNS 180

Query: 181 PSFSKVEADRMERIVKQGIFLLQCAMKWCLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNV 240
           PSFSKVEAD ++ IVKQGIFLLQCAMKW LTTGRQPLPMVAAVLVLVS LNEV+VSID+V
Sbjct: 181 PSFSKVEADTLDVIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSGLNEVNVSIDDV 240

Query: 241 ATEVHANVSTSKKRYRELLEALVEVGKKLPWGKDITIKNIVKNAPFVIQYMELKSMSNAS 300
           A EVHA VST KKRYRELL ALVEVGKKLPWGKDIT +NIVKNAPFVIQYMELKSMSN  
Sbjct: 241 AMEVHAKVSTCKKRYRELLGALVEVGKKLPWGKDITKRNIVKNAPFVIQYMELKSMSNTC 300

Query: 301 AKGKNLENVGIDLQVAVNDCLRKELEYESEVNNFEDDSQYFESRDNSWVDEPSCDDGNKL 360
            +GKN E+VGIDLQ AV++CLRKEL+YESEVNN EDDSQYFE   NSW DE SCDD NKL
Sbjct: 301 GRGKNFEDVGIDLQAAVSECLRKELQYESEVNNLEDDSQYFELIGNSWRDESSCDDRNKL 360

Query: 361 NISHECLSLIYNKFLNEMAHLRSSGGIAEVYGRKQGRKVAFHSCTEWWEGKSELSKKLML 420
           NISHECLSLIY KFL+EMAHLRSSGGI E+YGRK GRK  F S TEWWEGKS+LSKKL+L
Sbjct: 361 NISHECLSLIYTKFLDEMAHLRSSGGINEIYGRKLGRKAGFQSSTEWWEGKSQLSKKLLL 420

Query: 421 QQLLETDVGLNGVPPSFVSSCNAYKRRKEKINAAKKRIQRIMFPSNVVTSDVNVEKKRKR 480
            QLLETDVGLNG+PPSFV+SCNAYKRRKEKINAAK RIQRIM PSN    +VN EK+RKR
Sbjct: 421 HQLLETDVGLNGMPPSFVTSCNAYKRRKEKINAAKNRIQRIMLPSNTAIGNVNAEKERKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFSSWNS 526
           K A GIEWEDIIIETLLLH VKEEEIEKGHYN LLDLHVFSSW S
Sbjct: 481 KRAIGIEWEDIIIETLLLHDVKEEEIEKGHYNALLDLHVFSSWRS 525

BLAST of Spg017848 vs. ExPASy Swiss-Prot
Match: O81787 (Plant-specific TFIIB-related protein PTF2 OS=Arabidopsis thaliana OX=3702 GN=PTF2 PE=1 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 4.1e-122
Identity = 244/532 (45.86%), Postives = 339/532 (63.72%), Query Frame = 0

Query: 5   CNNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGVNGPQGTFIRVGTSGSGSVLIY 64
           C  C+  +  RD+ +GN  C  CG ++E+DNYEAQL G+ GPQGT+IRVGT G GSVL Y
Sbjct: 3   CKRCNGSNFERDEDTGNSYCGGCGTLREYDNYEAQLGGIRGPQGTYIRVGTIGRGSVLDY 62

Query: 65  KDKKIFEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFTILVGACAYVSMR 124
           KDKKI+EA  +IE+ T RL    +K+  ++ ++S +T+GE+G G+WF IL+GAC Y  +R
Sbjct: 63  KDKKIYEANNLIEETTERLNL-GNKTEVIKSMISKLTDGEFGQGEWFPILIGACCYAVVR 122

Query: 125 -KGNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGSEFPVFDIVGSLERAARNSPSF 184
            +G   L M EVA  + CDLH+LG M+ RVVD LDL   E   FD+VG   +   NSP  
Sbjct: 123 EEGKGVLSMEEVAYEVGCDLHQLGPMIKRVVDHLDL---ELREFDLVGLFTKTVTNSPRL 182

Query: 185 SKVEADRMERIVKQGIFLLQCAMKWCLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNVATE 244
           + V+ D+ E+I+KQG FL+ CA+KW L+TGR+P+P+V AVL  V ++N V V ID++A +
Sbjct: 183 TDVDRDKKEKIIKQGTFLMNCALKWFLSTGRRPMPLVVAVLAFVVQVNGVKVKIDDLAKD 242

Query: 245 VHANVSTSKKRYRELLEALVEVGKK--LPWGKDITIKNIVKNAPFVIQYMELKSM-SNAS 304
              +++T K RY+EL E LV+V ++  LPW KD+T+KN++K++  +   ME KSM     
Sbjct: 243 ASVSLTTCKTRYKELSEKLVKVAEEVGLPWAKDVTVKNVLKHSGTLFALMEAKSMKKRKQ 302

Query: 305 AKGKNLENV-GIDLQVAVNDCLRKELEYESEVNNFEDD-SQYFESRDNSWVDEPSCDDGN 364
             GK L    G  ++  V DCL KE  Y  + +  +D  S+YF+      +   + DD  
Sbjct: 303 GTGKELVRTDGFCVEDLVMDCLSKESMYCYDDDARQDTMSRYFDVEGERQLSLCNYDD-- 362

Query: 365 KLNISHECLSLIYNKFLNEMA----HLRSSGGIAEVYGRKQGRKVAFHSCTEWWEGKSEL 424
             NIS   LS  YN+F + +       RS G    ++ R+        S   WW+GKSEL
Sbjct: 363 --NISENQLSTKYNEFEDRVCGGTLAKRSQGSSQSMWQRRS--VFGMVSTENWWKGKSEL 422

Query: 425 SKKLMLQQLLETDVGLNGVPPSFVSSCNAYKRRKEKINAAKKRIQRIMFPS-NVVTSDVN 484
           SK+L+L+ LLE DVGL  +PPS++  C A +RR+EKI AAK RI  I  PS NV    ++
Sbjct: 423 SKRLLLKDLLEKDVGLEALPPSYIKGCVAVERRREKIKAAKLRINAIQHPSDNVSEGALS 482

Query: 485 VE---KKRKRKGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFSS 523
           +E    K+KRK  + I+WED++I+TL+LH V EEEIEKGHY  LLDLHVF+S
Sbjct: 483 LELEHSKKKRKKGSEIDWEDLVIQTLVLHNVNEEEIEKGHYKTLLDLHVFNS 524

BLAST of Spg017848 vs. ExPASy Swiss-Prot
Match: A8KBY2 (Transcription factor IIIB 50 kDa subunit OS=Danio rerio OX=7955 GN=brf2 PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 3.3e-07
Identity = 69/301 (22.92%), Postives = 124/301 (41.20%), Query Frame = 0

Query: 1   MSGSCNNCHSRSLFRDDV--SGNLICSSCGIVQEFDNYEAQLCGVNGPQGTFIRVGTSG- 60
           MS +C  C S  +  DD+      +C  CG V         L   +  +       T+  
Sbjct: 1   MSKNCPECGSSRVVEDDLYSQKQWVCEDCGSVVSEGLLTTTLSEESHSRAVPFFTSTAAF 60

Query: 61  --------SGSVLIYKDKKIFEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGD 120
                   SG   +    +IF     +ED +  L   A   N    L  ++++ +     
Sbjct: 61  KKPCRNLVSGFSRLRALCRIFRLSSSMEDASANLFERA--YNHPNFLHISLSKKQ----- 120

Query: 121 WFTILVGACAYVSMRKGNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGSEFPVFDI 180
              IL G C +   R+ + P+ M  +   ++ D +++G +   +   L+L+ ++  +  +
Sbjct: 121 ---ILAGCCMFHICRQNSWPVFMGTIGYLLDADNYQMGTIYQELTKSLNLQTTQVCITRM 180

Query: 181 VGSLERAARNSP-SFSKVEADRMERIVKQGIFLLQCAMKWCLTTGRQPLPM-VAAVLVLV 240
           + S     + +P    +V +   +R+V Q   LL+ A    + TGR+P P+ +AAV V  
Sbjct: 181 LESFCYDFKLAPDEVEEVFSVAQQRLVDQTSALLELAADTWILTGRRPFPLFLAAVYVAW 240

Query: 241 SKLN---EVDVSIDNVATEVHA-------NVSTSKKRYRELLEALVEVGKKLPWGKDITI 279
             LN    +  S+        A       +  T  KR  ELLE L ++G++LPW +   I
Sbjct: 241 QSLNPLARMKYSLMKFCKIAKAPEQLWCKSKDTINKRLNELLEVLCKLGRELPWVRPTDI 291

BLAST of Spg017848 vs. ExPASy Swiss-Prot
Match: Q979P7 (Transcription initiation factor IIB 2 OS=Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) OX=273116 GN=tfbB PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.1e-05
Identity = 64/302 (21.19%), Postives = 110/302 (36.42%), Query Frame = 0

Query: 5   CNNCHSRSLFRDDVSGNLICSSCGIVQE------------FDNYEAQLCGVNGPQGTFIR 64
           C  CHS  L RD   G LIC+ CG V E            FD+ + +     G   T++ 
Sbjct: 17  CPECHSEHLIRDYEHGELICADCGAVIEDSFIDQGPEWRAFDSDQDERRARTGSPMTYLS 76

Query: 65  ----VGTSGSGSVLIYKDKKIFE----------------------------AQKVIEDIT 124
               + T  S S   Y  K+I                              A +++ DI 
Sbjct: 77  HDKGLATEISWSNKDYYGKRIPHKNRAQIYRVRKWHQRIRVSNAAERNLSLALQLLNDIG 136

Query: 125 FRLGFSASKSNDVRILVSTITEGEYGLGDWFTILVGACAYVSMRKGNRPLPMSEVASTIE 184
            +LG          ++     E     G     +V A  Y + RK N P  + E+A   E
Sbjct: 137 AKLGIPKDIKETSALIYRKAVEKNLIRGRSIESIVCASIYAACRKVNIPRTLDEIAKASE 196

Query: 185 CDLHELGRMVLRVVDFLDLKGSEFPVFDIVGSLERAARNSPSFSKVEADRMERIVKQGIF 244
            +  ++G+    +   LDL       F  +             +K++ D+   ++ + I 
Sbjct: 197 VNKKKIGKAYRHLAKELDLNLRPTTPFSYIAQF---------CNKLDLDKQAIVISEDI- 256

Query: 245 LLQCAMKWCLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNVATEVHANVSTSKKRYRELLE 263
            ++ AM   +++G+ P  + AA + + S       +   +A        T + RY+E+ +
Sbjct: 257 -VRQAMSMGISSGKGPTGIAAAAIYIASVKVGKPRTQKEIARISGVTEVTIRNRYKEISK 307

BLAST of Spg017848 vs. ExPASy TrEMBL
Match: A0A5A7UMA0 (Transcription factor IIIB 50 kDa subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G002190 PE=4 SV=1)

HSP 1 Score: 908.3 bits (2346), Expect = 1.5e-260
Identity = 450/522 (86.21%), Postives = 486/522 (93.10%), Query Frame = 0

Query: 1   MSGSCNNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGVNGPQGTFIRVGTSGSGS 60
           MSGSC +C+SRS+FRDDVSGNLICSSCGIVQEF+NYEAQL G+NGPQGTF+RVGTSGSGS
Sbjct: 1   MSGSCKDCNSRSIFRDDVSGNLICSSCGIVQEFENYEAQLGGINGPQGTFVRVGTSGSGS 60

Query: 61  VLIYKDKKIFEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFTILVGACAY 120
           VL YKDKK++EAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWF ILVGACAY
Sbjct: 61  VLNYKDKKVYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKGNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGSEFPVFDIVGSLERAARNS 180
           VSMRK NRPL MSEVAS +ECDLHELGRMV+RVVDFLDL+GSEFPVFDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLSMSEVASAVECDLHELGRMVMRVVDFLDLRGSEFPVFDIVGSLERAARNS 180

Query: 181 PSFSKVEADRMERIVKQGIFLLQCAMKWCLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNV 240
           PSFS++EAD +ERIVKQGIFLLQCAMKW LTTGRQPLPMVAAVLVLVSKLNEVDVS++NV
Sbjct: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSMENV 240

Query: 241 ATEVHANVSTSKKRYRELLEALVEVGKKLPWGKDITIKNIVKNAPFVIQYMELKSMSNAS 300
           A EVHA VST KKRYRELLEALVEVGKKLPWGKDIT KNIVKNAPFVIQYMELKSMSNAS
Sbjct: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300

Query: 301 AKGKNLENVGIDLQVAVNDCLRKELEYESEVNNFEDDSQYFESRDNSWVDEPSCDDGNKL 360
            KGK+LENVGIDLQ AV++CLRKELEYESEVNN EDDSQYFE + + W DE +CD+GN+L
Sbjct: 301 GKGKDLENVGIDLQSAVSECLRKELEYESEVNNLEDDSQYFELQGSRWCDESNCDNGNRL 360

Query: 361 NISHECLSLIYNKFLNEMAHLRSSGGIAEVYGRKQGRKVAFHSCTEWWEGKSELSKKLML 420
           NISHECLSLIYNKFL+EMA LRSSGGI +VYGRKQGRK  F+S TEWWEGKS LSKKL+L
Sbjct: 361 NISHECLSLIYNKFLDEMAELRSSGGINDVYGRKQGRKAGFYSSTEWWEGKSVLSKKLLL 420

Query: 421 QQLLETDVGLNGVPPSFVSSCNAYKRRKEKINAAKKRIQRIMFPSNVVTSDVNVEKKRKR 480
           QQLLETD+G  G+PPSFVSSCNAYKRRKEK+NAAKKRIQRIM PSN    DVN++KKRKR
Sbjct: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIQRIMHPSNAPAGDVNIKKKRKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFSS 523
           KGA+ IEWEDIIIETLLLHGV+EEEIEKGHY VLLDLHVF+S
Sbjct: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLHVFTS 522

BLAST of Spg017848 vs. ExPASy TrEMBL
Match: A0A1S3B6X4 (transcription factor IIIB 50 kDa subunit OS=Cucumis melo OX=3656 GN=LOC103486826 PE=4 SV=1)

HSP 1 Score: 908.3 bits (2346), Expect = 1.5e-260
Identity = 450/522 (86.21%), Postives = 486/522 (93.10%), Query Frame = 0

Query: 1   MSGSCNNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGVNGPQGTFIRVGTSGSGS 60
           MSGSC +C+SRS+FRDDVSGNLICSSCGIVQEF+NYEAQL G+NGPQGTF+RVGTSGSGS
Sbjct: 1   MSGSCKDCNSRSIFRDDVSGNLICSSCGIVQEFENYEAQLGGINGPQGTFVRVGTSGSGS 60

Query: 61  VLIYKDKKIFEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFTILVGACAY 120
           VL YKDKK++EAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWF ILVGACAY
Sbjct: 61  VLNYKDKKVYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKGNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGSEFPVFDIVGSLERAARNS 180
           VSMRK NRPL MSEVAS +ECDLHELGRMV+RVVDFLDL+GSEFPVFDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLSMSEVASAVECDLHELGRMVMRVVDFLDLRGSEFPVFDIVGSLERAARNS 180

Query: 181 PSFSKVEADRMERIVKQGIFLLQCAMKWCLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNV 240
           PSFS++EAD +ERIVKQGIFLLQCAMKW LTTGRQPLPMVAAVLVLVSKLNEVDVS++NV
Sbjct: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSMENV 240

Query: 241 ATEVHANVSTSKKRYRELLEALVEVGKKLPWGKDITIKNIVKNAPFVIQYMELKSMSNAS 300
           A EVHA VST KKRYRELLEALVEVGKKLPWGKDIT KNIVKNAPFVIQYMELKSMSNAS
Sbjct: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300

Query: 301 AKGKNLENVGIDLQVAVNDCLRKELEYESEVNNFEDDSQYFESRDNSWVDEPSCDDGNKL 360
            KGK+LENVGIDLQ AV++CLRKELEYESEVNN EDDSQYFE + + W DE +CD+GN+L
Sbjct: 301 GKGKDLENVGIDLQSAVSECLRKELEYESEVNNLEDDSQYFELQGSRWCDESNCDNGNRL 360

Query: 361 NISHECLSLIYNKFLNEMAHLRSSGGIAEVYGRKQGRKVAFHSCTEWWEGKSELSKKLML 420
           NISHECLSLIYNKFL+EMA LRSSGGI +VYGRKQGRK  F+S TEWWEGKS LSKKL+L
Sbjct: 361 NISHECLSLIYNKFLDEMAELRSSGGINDVYGRKQGRKAGFYSSTEWWEGKSVLSKKLLL 420

Query: 421 QQLLETDVGLNGVPPSFVSSCNAYKRRKEKINAAKKRIQRIMFPSNVVTSDVNVEKKRKR 480
           QQLLETD+G  G+PPSFVSSCNAYKRRKEK+NAAKKRIQRIM PSN    DVN++KKRKR
Sbjct: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIQRIMHPSNAPAGDVNIKKKRKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFSS 523
           KGA+ IEWEDIIIETLLLHGV+EEEIEKGHY VLLDLHVF+S
Sbjct: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLHVFTS 522

BLAST of Spg017848 vs. ExPASy TrEMBL
Match: A0A6J1GSR8 (plant-specific TFIIB-related protein PTF2 OS=Cucurbita moschata OX=3662 GN=LOC111457096 PE=4 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 5.0e-256
Identity = 448/525 (85.33%), Postives = 473/525 (90.10%), Query Frame = 0

Query: 1   MSGSCNNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGVNGPQGTFIRVGTSGSGS 60
           MSGSC NC  RSLFRDDVSGNLICSSCGIVQEFDNY+AQL G+NGPQGTF+RVGTSGSGS
Sbjct: 1   MSGSCKNCSGRSLFRDDVSGNLICSSCGIVQEFDNYDAQLGGLNGPQGTFVRVGTSGSGS 60

Query: 61  VLIYKDKKIFEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFTILVGACAY 120
           VL YKDKKI+EAQ +IEDITFRLGFSASKSNDVRILVSTITEGEYG GDWF+ILVGAC+Y
Sbjct: 61  VLNYKDKKIYEAQNIIEDITFRLGFSASKSNDVRILVSTITEGEYGQGDWFSILVGACSY 120

Query: 121 VSMRKGNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGSEFPVFDIVGSLERAARNS 180
           VSMRK NRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKG  FP FDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGPAFPGFDIVGSLERAARNS 180

Query: 181 PSFSKVEADRMERIVKQGIFLLQCAMKWCLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNV 240
           PSFSKVEAD ++ IVKQGIFLLQCAMKW LTTGRQPLPMVAAVLVLVS LNEV+VSID+V
Sbjct: 181 PSFSKVEADTLDVIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSGLNEVNVSIDDV 240

Query: 241 ATEVHANVSTSKKRYRELLEALVEVGKKLPWGKDITIKNIVKNAPFVIQYMELKSMSNAS 300
           A EVHA VST KKRYRELL ALVEVGKKLPWGKDIT +NIVKNAPFVIQYMELKSMSN  
Sbjct: 241 AMEVHAKVSTCKKRYRELLGALVEVGKKLPWGKDITKRNIVKNAPFVIQYMELKSMSNTC 300

Query: 301 AKGKNLENVGIDLQVAVNDCLRKELEYESEVNNFEDDSQYFESRDNSWVDEPSCDDGNKL 360
            +GKN E+VGIDLQ AV++CLRKEL+YESEVNN EDDSQYFE   NSW DE SCDD NKL
Sbjct: 301 GRGKNFEDVGIDLQAAVSECLRKELQYESEVNNLEDDSQYFELIGNSWRDESSCDDRNKL 360

Query: 361 NISHECLSLIYNKFLNEMAHLRSSGGIAEVYGRKQGRKVAFHSCTEWWEGKSELSKKLML 420
           NISHECLSLIY KFL+EMAHLRSSGGI E+YGRK GRK  F S TEWWEGKS+LSKKL+L
Sbjct: 361 NISHECLSLIYTKFLDEMAHLRSSGGINEIYGRKLGRKAGFQSSTEWWEGKSQLSKKLLL 420

Query: 421 QQLLETDVGLNGVPPSFVSSCNAYKRRKEKINAAKKRIQRIMFPSNVVTSDVNVEKKRKR 480
            QLLETDVGLNG+PPSFV+SCNAYKRRKEKINAAK RIQRIM PSN    +VN EK+RKR
Sbjct: 421 HQLLETDVGLNGMPPSFVTSCNAYKRRKEKINAAKNRIQRIMLPSNTAIGNVNAEKERKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFSSWNS 526
           K A GIEWEDIIIETLLLH VKEEEIEKGHYN LLDLHVFSSW S
Sbjct: 481 KRAIGIEWEDIIIETLLLHDVKEEEIEKGHYNALLDLHVFSSWRS 525

BLAST of Spg017848 vs. ExPASy TrEMBL
Match: A0A6J1JZD6 (plant-specific TFIIB-related protein PTF2 OS=Cucurbita maxima OX=3661 GN=LOC111490217 PE=4 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.5e-255
Identity = 448/525 (85.33%), Postives = 473/525 (90.10%), Query Frame = 0

Query: 1   MSGSCNNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGVNGPQGTFIRVGTSGSGS 60
           MSGSC NC  RSLFRDDVSGNLICSSCGIVQEFDNY+AQL G+NGPQGTF+RVGTSGSGS
Sbjct: 1   MSGSCKNCSGRSLFRDDVSGNLICSSCGIVQEFDNYDAQLGGLNGPQGTFVRVGTSGSGS 60

Query: 61  VLIYKDKKIFEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFTILVGACAY 120
           VL YKDKKI+EAQK+IEDITFRLGFSASKSNDVRILVSTITEGEYG GDWF+ILVGAC+Y
Sbjct: 61  VLNYKDKKIYEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGQGDWFSILVGACSY 120

Query: 121 VSMRKGNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGSEFPVFDIVGSLERAARNS 180
           VSMRK NRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKG  FP FDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGPAFPGFDIVGSLERAARNS 180

Query: 181 PSFSKVEADRMERIVKQGIFLLQCAMKWCLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNV 240
           PSFSKVEAD ++ IVKQGIFLLQCAMKW LTTGRQPLPMVAAVLVLVS LNEV+VSID+V
Sbjct: 181 PSFSKVEADTLDIIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSGLNEVNVSIDDV 240

Query: 241 ATEVHANVSTSKKRYRELLEALVEVGKKLPWGKDITIKNIVKNAPFVIQYMELKSMSNAS 300
           A EVHA VST KKRYRELL ALVEVGKKLPWGKDIT +NIVKNAPFVIQYMELKSMSN  
Sbjct: 241 AMEVHAKVSTCKKRYRELLGALVEVGKKLPWGKDITKRNIVKNAPFVIQYMELKSMSNTC 300

Query: 301 AKGKNLENVGIDLQVAVNDCLRKELEYESEVNNFEDDSQYFESRDNSWVDEPSCDDGNKL 360
            +GKN E+VGIDLQ AV++CLRKEL+YESEVNN EDDSQYFE   NSW DE SCDD NKL
Sbjct: 301 GQGKNFEDVGIDLQAAVSECLRKELQYESEVNNLEDDSQYFELIGNSWHDESSCDDRNKL 360

Query: 361 NISHECLSLIYNKFLNEMAHLRSSGGIAEVYGRKQGRKVAFHSCTEWWEGKSELSKKLML 420
           NISHECLSLIY KFL+EMAHLRSSG I E+YGRK GRK  F S TEWWEGKS+LSKKL+L
Sbjct: 361 NISHECLSLIYTKFLDEMAHLRSSGEINEIYGRKLGRKAGFQSSTEWWEGKSQLSKKLLL 420

Query: 421 QQLLETDVGLNGVPPSFVSSCNAYKRRKEKINAAKKRIQRIMFPSNVVTSDVNVEKKRKR 480
            QLLETDVGLNG+PPSFV+SCNAYKRRKEKINAAK RIQRIM PS+    DVN EK+RKR
Sbjct: 421 HQLLETDVGLNGMPPSFVTSCNAYKRRKEKINAAKNRIQRIMLPSHAAIGDVNAEKERKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFSSWNS 526
           K A GIEWEDIIIETLLLH VKEEEIEKGHYN LLDLHVFSSW S
Sbjct: 481 KRAVGIEWEDIIIETLLLHDVKEEEIEKGHYNALLDLHVFSSWRS 525

BLAST of Spg017848 vs. ExPASy TrEMBL
Match: A0A0A0M0A0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G477510 PE=4 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.9e-255
Identity = 445/522 (85.25%), Postives = 480/522 (91.95%), Query Frame = 0

Query: 1   MSGSCNNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGVNGPQGTFIRVGTSGSGS 60
           MSGSC NCHSRS+FRDDVSGN ICSSCGIVQEFDNY+AQL G+NGPQGTF+RVGTSGSGS
Sbjct: 1   MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS 60

Query: 61  VLIYKDKKIFEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFTILVGACAY 120
           VL YKDKKI+EAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWF ILVGACAY
Sbjct: 61  VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKGNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGSEFPVFDIVGSLERAARNS 180
           VSMRK +RPL MSEVAS +ECDLHELGRMV+RVV+FLDL+GSEFPVFDIVGSLERAARNS
Sbjct: 121 VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS 180

Query: 181 PSFSKVEADRMERIVKQGIFLLQCAMKWCLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNV 240
           PSFS++EAD +ERIVKQGIFLLQCAMKW LTTGRQPLPMVAAVLVLVSKLNEVDVSI+NV
Sbjct: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV 240

Query: 241 ATEVHANVSTSKKRYRELLEALVEVGKKLPWGKDITIKNIVKNAPFVIQYMELKSMSNAS 300
             EVHANVST KKRYRELLEALVEVGKKLPWGKDIT KNIVKNAPFVIQYMELKSMS AS
Sbjct: 241 GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS 300

Query: 301 AKGKNLENVGIDLQVAVNDCLRKELEYESEVNNFEDDSQYFESRDNSWVDEPSCDDGNKL 360
            KGK+LENV IDLQ AV++CLRKELEYESEV N EDDSQYFE + + W DE + D+GN+L
Sbjct: 301 GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL 360

Query: 361 NISHECLSLIYNKFLNEMAHLRSSGGIAEVYGRKQGRKVAFHSCTEWWEGKSELSKKLML 420
           NISHECLSLIYNKFL+EMA LRSSGGI EVYG KQGRK  F+S TEWWEGKSELSKKL+L
Sbjct: 361 NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL 420

Query: 421 QQLLETDVGLNGVPPSFVSSCNAYKRRKEKINAAKKRIQRIMFPSNVVTSDVNVEKKRKR 480
           QQLLETD+G  G+PPSFVSSCNAY+RRKEK+NAAKKRIQRIM PS     DVN++KKRKR
Sbjct: 421 QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFSS 523
           KGA+ IEWEDIIIETLLLHGV+EEEIEKGHY VLLDL+VF+S
Sbjct: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTS 522

BLAST of Spg017848 vs. TAIR 10
Match: AT4G35540.1 (zinc ion binding;transcription regulators )

HSP 1 Score: 439.9 bits (1130), Expect = 2.9e-123
Identity = 244/532 (45.86%), Postives = 339/532 (63.72%), Query Frame = 0

Query: 5   CNNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGVNGPQGTFIRVGTSGSGSVLIY 64
           C  C+  +  RD+ +GN  C  CG ++E+DNYEAQL G+ GPQGT+IRVGT G GSVL Y
Sbjct: 3   CKRCNGSNFERDEDTGNSYCGGCGTLREYDNYEAQLGGIRGPQGTYIRVGTIGRGSVLDY 62

Query: 65  KDKKIFEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFTILVGACAYVSMR 124
           KDKKI+EA  +IE+ T RL    +K+  ++ ++S +T+GE+G G+WF IL+GAC Y  +R
Sbjct: 63  KDKKIYEANNLIEETTERLNL-GNKTEVIKSMISKLTDGEFGQGEWFPILIGACCYAVVR 122

Query: 125 -KGNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGSEFPVFDIVGSLERAARNSPSF 184
            +G   L M EVA  + CDLH+LG M+ RVVD LDL   E   FD+VG   +   NSP  
Sbjct: 123 EEGKGVLSMEEVAYEVGCDLHQLGPMIKRVVDHLDL---ELREFDLVGLFTKTVTNSPRL 182

Query: 185 SKVEADRMERIVKQGIFLLQCAMKWCLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNVATE 244
           + V+ D+ E+I+KQG FL+ CA+KW L+TGR+P+P+V AVL  V ++N V V ID++A +
Sbjct: 183 TDVDRDKKEKIIKQGTFLMNCALKWFLSTGRRPMPLVVAVLAFVVQVNGVKVKIDDLAKD 242

Query: 245 VHANVSTSKKRYRELLEALVEVGKK--LPWGKDITIKNIVKNAPFVIQYMELKSM-SNAS 304
              +++T K RY+EL E LV+V ++  LPW KD+T+KN++K++  +   ME KSM     
Sbjct: 243 ASVSLTTCKTRYKELSEKLVKVAEEVGLPWAKDVTVKNVLKHSGTLFALMEAKSMKKRKQ 302

Query: 305 AKGKNLENV-GIDLQVAVNDCLRKELEYESEVNNFEDD-SQYFESRDNSWVDEPSCDDGN 364
             GK L    G  ++  V DCL KE  Y  + +  +D  S+YF+      +   + DD  
Sbjct: 303 GTGKELVRTDGFCVEDLVMDCLSKESMYCYDDDARQDTMSRYFDVEGERQLSLCNYDD-- 362

Query: 365 KLNISHECLSLIYNKFLNEMA----HLRSSGGIAEVYGRKQGRKVAFHSCTEWWEGKSEL 424
             NIS   LS  YN+F + +       RS G    ++ R+        S   WW+GKSEL
Sbjct: 363 --NISENQLSTKYNEFEDRVCGGTLAKRSQGSSQSMWQRRS--VFGMVSTENWWKGKSEL 422

Query: 425 SKKLMLQQLLETDVGLNGVPPSFVSSCNAYKRRKEKINAAKKRIQRIMFPS-NVVTSDVN 484
           SK+L+L+ LLE DVGL  +PPS++  C A +RR+EKI AAK RI  I  PS NV    ++
Sbjct: 423 SKRLLLKDLLEKDVGLEALPPSYIKGCVAVERRREKIKAAKLRINAIQHPSDNVSEGALS 482

Query: 485 VE---KKRKRKGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFSS 523
           +E    K+KRK  + I+WED++I+TL+LH V EEEIEKGHY  LLDLHVF+S
Sbjct: 483 LELEHSKKKRKKGSEIDWEDLVIQTLVLHNVNEEEIEKGHYKTLLDLHVFNS 524

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893651.11.2e-26487.91plant-specific TFIIB-related protein PTF2 [Benincasa hispida][more]
XP_008443157.13.1e-26086.21PREDICTED: transcription factor IIIB 50 kDa subunit [Cucumis melo] >KAA0057033.1... [more]
XP_023542889.12.1e-25685.52plant-specific TFIIB-related protein PTF2 [Cucurbita pepo subsp. pepo][more]
KAG6573303.14.6e-25685.33Plant-specific TFIIB-related protein PTF2, partial [Cucurbita argyrosperma subsp... [more]
XP_022955013.11.0e-25585.33plant-specific TFIIB-related protein PTF2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O817874.1e-12245.86Plant-specific TFIIB-related protein PTF2 OS=Arabidopsis thaliana OX=3702 GN=PTF... [more]
A8KBY23.3e-0722.92Transcription factor IIIB 50 kDa subunit OS=Danio rerio OX=7955 GN=brf2 PE=2 SV=... [more]
Q979P71.1e-0521.19Transcription initiation factor IIB 2 OS=Thermoplasma volcanium (strain ATCC 515... [more]
Match NameE-valueIdentityDescription
A0A5A7UMA01.5e-26086.21Transcription factor IIIB 50 kDa subunit OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3B6X41.5e-26086.21transcription factor IIIB 50 kDa subunit OS=Cucumis melo OX=3656 GN=LOC103486826... [more]
A0A6J1GSR85.0e-25685.33plant-specific TFIIB-related protein PTF2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JZD61.5e-25585.33plant-specific TFIIB-related protein PTF2 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A0A0M0A01.9e-25585.25Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G477510 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G35540.12.9e-12345.86zinc ion binding;transcription regulators [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.472.10coord: 182..265
e-value: 2.8E-8
score: 35.9
NoneNo IPR availableGENE3D2.20.25.10coord: 2..37
e-value: 2.0E-5
score: 25.8
NoneNo IPR availableGENE3D1.10.472.10coord: 64..162
e-value: 2.2E-6
score: 29.6
NoneNo IPR availablePANTHERPTHR11618:SF13TRANSCRIPTION INITIATION FACTOR IIBcoord: 3..297
NoneNo IPR availableSUPERFAMILY57783Zinc beta-ribboncoord: 4..33
IPR000812Transcription factor TFIIBPANTHERPTHR11618TRANSCRIPTION INITIATION FACTOR IIB-RELATEDcoord: 3..297
IPR036915Cyclin-like superfamilySUPERFAMILY47954Cyclin-likecoord: 72..160
IPR036915Cyclin-like superfamilySUPERFAMILY47954Cyclin-likecoord: 194..262

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg017848.1Spg017848.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070897 transcription preinitiation complex assembly
biological_process GO:0006352 DNA-templated transcription, initiation
cellular_component GO:0005634 nucleus
cellular_component GO:0097550 transcription preinitiation complex
molecular_function GO:0017025 TBP-class protein binding