Spg017623 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg017623 vs. NCBI nr
Match: XP_038880237.1 (uncharacterized vacuolar membrane protein YML018C-like [Benincasa hispida])

HSP 1 Score: 377.1 bits (967), Expect = 1.5e-100
Identity = 192/330 (58.18%), Postives = 251/330 (76.06%), Query Frame = 0

Query: 1   MEVQISKEEVESSKTCNDNKRQRSLT-----NDQCVV------DIEARENGNDIICKCEG 60
           +E + ++  +E     N+N     ++     + QCVV      D+    N ++  C+CE 
Sbjct: 86  LEERTNQSSIEDDDNNNNNNNNNIISEVGNGDVQCVVNIIQGEDLRVNNNNHNGGCECEP 145

Query: 61  EVID---IPKKERKLDTKEVALLAICIGPLWFTSEYLTSCALAHTSVATTTILFSTSGLF 120
           E ++   +  +E K  TK++A+LA+ IGP+WF SEY T+ ALA TSVATTTILFSTSGLF
Sbjct: 146 ENLEMTILKTEETKCSTKQIAVLALVIGPIWFVSEYFTNAALARTSVATTTILFSTSGLF 205

Query: 121 TLVIDGFF-KKSINGVNVISVIISMAGVAMTTTAKTWARDE----PTLNEKHSLVGDAFA 180
           TL+ID    ++S+  VNV++VI+SMAGVAMTT  KTWARDE     + + KHS VGD FA
Sbjct: 206 TLIIDACLERQSLTIVNVVAVIVSMAGVAMTTVGKTWARDEVRSSSSEHGKHSYVGDIFA 265

Query: 181 LLSALTDGLYYVLLNKYAGENGQKLDLQKFLGFVGLFVFSGLWWLVWPLTALGIEPKFAL 240
           LLSALTDGLYYVLL KYAGE G+K+D+QKFLG+VGLF  + LWWL+WPL A+GIEPKF +
Sbjct: 266 LLSALTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFMI 325

Query: 241 PKSAKLMEVIVANCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLTDMLIHGRHY 300
           P+S K+ E+++ANCF+ NFV+DYFWA+G +WTSPLVAALG+SLTIPLAML DM++HGRHY
Sbjct: 326 PQSTKVAELVLANCFVSNFVSDYFWAMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHY 385

Query: 301 SLLYILGSIQVFVGFIAANLSDWLTPKLRL 312
           SL+YI GS+QVF+GF+ ANLSDW++PKL+L
Sbjct: 386 SLIYIFGSVQVFLGFMIANLSDWISPKLKL 415

BLAST of Spg017623 vs. NCBI nr
Match: XP_023531720.1 (uncharacterized transporter C405.03c-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 374.0 bits (959), Expect = 1.3e-99
Identity = 190/325 (58.46%), Postives = 245/325 (75.38%), Query Frame = 0

Query: 2   EVQISKEEVESSKTCNDNKRQRSLTND------QCVVDIEAREN-----GNDIICKCEGE 61
           E+Q       ++ T N+N    +  ++       CVV+IE  E       N+  C+CE E
Sbjct: 78  EIQEQDNNNNNNNTNNNNNNNNNNISEVENGDVHCVVNIECEEQEKGSVSNNGRCECEAE 137

Query: 62  VIDIPKKERKLDTKEVALLAICIGPLWFTSEYLTSCALAHTSVATTTILFSTSGLFTLVI 121
                 +E    TK++A+LA+ IGP+WF SEY T+ ALA TSVATTTILFSTSGLFTL++
Sbjct: 138 ---NGTQETPFSTKQMAVLALAIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIL 197

Query: 122 DGFF-KKSINGVNVISVIISMAGVAMTTTAKTWARDEPTL---NEKHSLVGDAFALLSAL 181
           D  F ++S++ VNV++V +SMAGVAMTT  KTWARDE      +EKHS +GD FALLSA 
Sbjct: 198 DACFERQSLSIVNVVAVFVSMAGVAMTTVGKTWARDETQSSFGHEKHSYIGDVFALLSAF 257

Query: 182 TDGLYYVLLNKYAGENGQKLDLQKFLGFVGLFVFSGLWWLVWPLTALGIEPKFALPKSAK 241
           TDGLYYVLL KYAGE G+K+D+QKFLG+VGLF  + LWWL+WPL A+GIEPKF +P+S K
Sbjct: 258 TDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTLTTLWWLIWPLRAMGIEPKFGIPQSTK 317

Query: 242 LMEVIVANCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLTDMLIHGRHYSLLYI 301
           + E+++ANCF+ NFV+DYFWA+G +WTSPLVAALG+SLTIPLAM+ DML+HGRHYSL+YI
Sbjct: 318 VAEIVLANCFVSNFVSDYFWAMGVVWTSPLVAALGASLTIPLAMVGDMLLHGRHYSLVYI 377

Query: 302 LGSIQVFVGFIAANLSDWLTPKLRL 312
            GS+QVF+GF+ AN SDW++PKL+L
Sbjct: 378 FGSLQVFLGFMIANFSDWISPKLKL 399

BLAST of Spg017623 vs. NCBI nr
Match: XP_022134812.1 (uncharacterized transporter C405.03c-like [Momordica charantia])

HSP 1 Score: 373.6 bits (958), Expect = 1.7e-99
Identity = 193/325 (59.38%), Postives = 248/325 (76.31%), Query Frame = 0

Query: 2   EVQISKEEVESSKTCNDNKRQRSLTND----QCVVDIEARE------NGNDIICKCEGEV 61
           +V   K    SS   N+N    S   D     CVV I+  E        N   C+CE + 
Sbjct: 83  QVMEEKSNNPSSPNNNNNNNNISEVGDGSEVHCVVQIQCEEEVVSVDKDNYGGCECENQE 142

Query: 62  IDIPKKERKLDTKEVALLAICIGPLWFTSEYLTSCALAHTSVATTTILFSTSGLFTLVID 121
           + +  K ++    + A+LA+ IGP+WF SEY T+ ALA TSVATTTILFSTSGLFTL+ID
Sbjct: 143 VAMALKTKE---TKFAVLALTIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIID 202

Query: 122 GFF-KKSINGVNVISVIISMAGVAMTTTAKTWARDEP--TLNE--KHSLVGDAFALLSAL 181
            F  ++S+  VN+++V++S+AGVAMTT  KTWA+D+P  T +E  KHS VGDAFALLSAL
Sbjct: 203 AFLERQSLTIVNIVAVVVSLAGVAMTTIGKTWAKDQPHSTSSEHGKHSFVGDAFALLSAL 262

Query: 182 TDGLYYVLLNKYAGENGQKLDLQKFLGFVGLFVFSGLWWLVWPLTALGIEPKFALPKSAK 241
           TDGLYYVLL KYAGE G+K+D+QKFLG+VGLF  + LWWL+WPL A+GIEPKFA+P+S K
Sbjct: 263 TDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFAIPRSTK 322

Query: 242 LMEVIVANCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLTDMLIHGRHYSLLYI 301
           + E+++ANCF+GNF++DYFWA+G +WTSPLVAALG+SLTIPLAML DM++HGRHYSL+YI
Sbjct: 323 VAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYI 382

Query: 302 LGSIQVFVGFIAANLSDWLTPKLRL 312
            GS+QVF+GF+ ANLSDW++PKL+L
Sbjct: 383 FGSLQVFLGFMIANLSDWISPKLKL 404

BLAST of Spg017623 vs. NCBI nr
Match: XP_022925420.1 (uncharacterized transporter C405.03c-like [Cucurbita moschata])

HSP 1 Score: 372.9 bits (956), Expect = 2.8e-99
Identity = 187/318 (58.81%), Postives = 244/318 (76.73%), Query Frame = 0

Query: 8   EEVESSKTCNDNKRQRSLTNDQ-----CVVDIEAREN-----GNDIICKCEGEVIDIPKK 67
           +E +++   N+N    +++  +     CVV++E  E       N+  C+CE E      +
Sbjct: 80  QEQDNNNNNNNNNNNNNISEVENGDVHCVVNVECEEQEKGSASNNGRCECEAE---NGTQ 139

Query: 68  ERKLDTKEVALLAICIGPLWFTSEYLTSCALAHTSVATTTILFSTSGLFTLVIDGFF-KK 127
           E    TK++A+LA+ IGP+WF SEY T+ ALA TSVATTTILFSTSGLFTL++D  F ++
Sbjct: 140 ETSFSTKQMAVLALAIGPIWFVSEYFTNAALAKTSVATTTILFSTSGLFTLILDACFERQ 199

Query: 128 SINGVNVISVIISMAGVAMTTTAKTWARDEPTL---NEKHSLVGDAFALLSALTDGLYYV 187
           S++ VNV++V +SMAGVAMTT  KTWARDE      +EKHS +GD FALLSA TDGLYYV
Sbjct: 200 SLSIVNVVAVFVSMAGVAMTTVGKTWARDEAQSSFGHEKHSYIGDVFALLSAFTDGLYYV 259

Query: 188 LLNKYAGENGQKLDLQKFLGFVGLFVFSGLWWLVWPLTALGIEPKFALPKSAKLMEVIVA 247
           LL KYAGE G+K+D+QKFLG+VGLF  + LWWL+WPL A+GIEPKF LP+S K+ E+++A
Sbjct: 260 LLKKYAGEEGEKVDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFGLPQSTKVAEIVLA 319

Query: 248 NCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLTDMLIHGRHYSLLYILGSIQVF 307
           NCF+ NFV+DY WA+G +WTSPLVAALG+SLTIPLAM+ DML+HGRHYSL+YI GS+QVF
Sbjct: 320 NCFVSNFVSDYLWAMGVVWTSPLVAALGASLTIPLAMVGDMLLHGRHYSLVYIFGSLQVF 379

Query: 308 VGFIAANLSDWLTPKLRL 312
           +GF+ AN SDW++PKL+L
Sbjct: 380 LGFMIANFSDWISPKLKL 394

BLAST of Spg017623 vs. NCBI nr
Match: TYK06345.1 (putative transporter-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 372.5 bits (955), Expect = 3.7e-99
Identity = 188/300 (62.67%), Postives = 233/300 (77.67%), Query Frame = 0

Query: 17  NDNKRQRSLTNDQCVVDIEARENGNDIICKCEGEVIDIPKKERKLDTKEVALLAICIGPL 76
           N+N    S  N  C  ++   EN    I K E         E K  TK++A+LA+ IGP+
Sbjct: 121 NNNSNNSSNNNGGC--ELREAENMEMRILKTE---------EAKFSTKQIAVLALTIGPI 180

Query: 77  WFTSEYLTSCALAHTSVATTTILFSTSGLFTLVIDGFF-KKSINGVNVISVIISMAGVAM 136
           WF SEY T+ ALA TSVATTTILFSTSGLFTL++D    ++S+  VNV++V++SMAGVAM
Sbjct: 181 WFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACLERQSLTIVNVVAVVVSMAGVAM 240

Query: 137 TTTAKTWARDEPTLNE----KHSLVGDAFALLSALTDGLYYVLLNKYAGENGQKLDLQKF 196
           TT  KTWA+DEP  +     KHS VGDAFALLSALTDGLYYVLL KYAGE G+K+D+QKF
Sbjct: 241 TTVGKTWAKDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKVDMQKF 300

Query: 197 LGFVGLFVFSGLWWLVWPLTALGIEPKFALPKSAKLMEVIVANCFLGNFVADYFWALGAI 256
           LG+VGLF  + LWWL+WPL A+GIEPKF +P+S K+ EV++ANCF+ NFV+DYFWA+G +
Sbjct: 301 LGYVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFVANFVSDYFWAMGVV 360

Query: 257 WTSPLVAALGSSLTIPLAMLTDMLIHGRHYSLLYILGSIQVFVGFIAANLSDWLTPKLRL 312
           WTSPLVAALG+SLTIPLAM+ DM++HGRHYSL+YI GSIQVF+GFI AN SDW++PKL+L
Sbjct: 361 WTSPLVAALGASLTIPLAMIGDMVLHGRHYSLVYIFGSIQVFLGFIIANFSDWISPKLKL 409

BLAST of Spg017623 vs. ExPASy Swiss-Prot
Match: Q03730 (Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YML018C PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 5.1e-27
Identity = 95/302 (31.46%), Postives = 153/302 (50.66%), Query Frame = 0

Query: 10  VESSKTCNDNKRQRSLTNDQCVVDIEARENGNDIICKCEGEVIDIPKKERKLDTKEVALL 69
           +E   T +D+ R   +T+   + ++EA  + N               ++++L   E   L
Sbjct: 86  MEEEGTGSDSNRSVDMTS-PLLTNLEAGTHAN---------------QKKRLTLYETIKL 145

Query: 70  AICIGPLWFTSEYLTSCALAHTSVATTTILFSTSGLFTLVIDGF-FKKSINGVNVISVII 129
           +     LWFT+  +T+ +LA TSVA+ TIL +TS  FTL I      +S++   V+   I
Sbjct: 146 SAEFCILWFTANLVTNASLAFTSVASQTILSTTSSFFTLFIGAICHVESLSKSKVLGSFI 205

Query: 130 SMAGVAMTTTAKTWARDEPTLNEKHS--------LVGDAFALLSALTDGLYYVLLNKYAG 189
           S  G+ M T + +  R +  + +           L+G+  AL  A+  G+Y  LL +  G
Sbjct: 206 SFVGIIMVTKSDSHQRYQRHIADVSGDDNDAVQVLIGNLLALAGAVLYGVYSTLLKREVG 265

Query: 190 ENGQKLDLQKFLGFVGLFVFSGLWWLVWPLTALGIEPKFALPKSAKLMEVIVANCFLGNF 249
           +   +++++ F GFVGLF    LW  +  L   G EP F+LPK  K++ +I  NC +  F
Sbjct: 266 DE-TRVNMKIFFGFVGLFNLLFLWPSLIVLDFFGWEP-FSLPKDPKVVVIIFVNCLI-TF 325

Query: 250 VADYFWALGAIWTSPLVAALGSSLTIPLAMLTDMLIHGRHYSLLYILGSIQVFVGFIAAN 303
           V+D+ WA   + TSPL   +G S+TIPLAM  D++   +  S LY+ G+  +   F   N
Sbjct: 326 VSDFCWAKAMLLTSPLTVTVGLSITIPLAMFGDVIFKHKTMSALYLFGATLILGSFFIIN 368

BLAST of Spg017623 vs. ExPASy Swiss-Prot
Match: Q04083 (Thiamine-repressible mitochondrial transport protein THI74 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=THI74 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.5e-26
Identity = 80/240 (33.33%), Postives = 131/240 (54.58%), Query Frame = 0

Query: 62  DTKEVALLAICIGPLWFTSEYLTSCALAHTSVATTTILFSTSGLFTLVI-DGFFKKSING 121
           DT  ++LL  C+  LWF +    + AL++T+VA++TIL STS  FTL +      ++ + 
Sbjct: 116 DTMRLSLL-FCV--LWFVANLAANAALSYTTVASSTILSSTSSFFTLFLATSLGIETFST 175

Query: 122 VNVISVIISMAGVAMTTTAKTWARDEPTLNEKHSLVGDAFALLSALTDGLYYVLLNKYAG 181
             ++ + +S+ G+ +       ++ + +++    LVG+  ALL +L   +Y  LL     
Sbjct: 176 KKLLGLFVSLFGIILIVMQS--SKQQDSVSASSFLVGNTLALLGSLGYSVYTTLLKYEIS 235

Query: 182 ENGQKLDLQKFLGFVGLFVFSGLWWLVWPLTALGIEPKFALPKSAKLMEVIVANCFLGNF 241
             G +LD+Q FLG+VG+F F   W ++  L    +E  F LP +  +  +++ NC +  F
Sbjct: 236 SKGLRLDIQMFLGYVGIFTFLLFWPILIILDITHME-TFELPSNFHISFLVMLNCII-IF 295

Query: 242 VADYFWALGAIWTSPLVAALGSSLTIPLAMLTDMLIHGRHYSLLYILGSIQVFVGFIAAN 301
           V+DYFW    I TSPLV  +  + TIPLAM  D +     ++  YI+G I +FV F   N
Sbjct: 296 VSDYFWCKALILTSPLVVTVALTFTIPLAMFADFVWREAFFTPWYIIGVIFIFVSFFLVN 348

BLAST of Spg017623 vs. ExPASy Swiss-Prot
Match: O94654 (Uncharacterized transporter C405.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC405.03c PE=3 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.2e-25
Identity = 79/241 (32.78%), Postives = 130/241 (53.94%), Query Frame = 0

Query: 61  LDTKEVALLAICIGPLWFTSEYLTSCALAHTSVATTTILFSTSGLFTLVIDGFFK-KSIN 120
           L  ++ A L++    +WF + Y ++ +L  T+VA+ TI+ S SG FTL +      +   
Sbjct: 97  LGFRQTAYLSLGFCIIWFAANYFSNSSLGFTNVASFTIISSMSGFFTLGLGTIVNVERFT 156

Query: 121 GVNVISVIISMAGVAMTTTAKTWARDEPTLNEKHSLVGDAFALLSALTDGLYYVLLNKYA 180
              +++++ S+ GV +  T      D          +G+A+ALL+AL  G Y V++  + 
Sbjct: 157 LSKLLALMASVGGVIIVVTQDAKQADLNDSPPSRPALGNAYALLAALLYGCYSVMVKFHI 216

Query: 181 GENGQKLDLQKFLGFVGLFVFSGLWWLVWPLTALGIEPKFALPKSAKLMEVIVANCFLGN 240
            E    +  + F G VGLF    LW  +  L   G+E +F+LP +   + V++ N  +  
Sbjct: 217 TEE-SCVSTRLFFGLVGLFDLILLWPFLIILHLYGVE-RFSLPSTTAGLIVLIINASI-T 276

Query: 241 FVADYFWALGAIWTSPLVAALGSSLTIPLAMLTDMLIHGRHYSLLYILGSIQVFVGFIAA 300
           FV+DY W +  + TSPL+  +G SL+IPLA+  D+L+ G + +   ILGS+ VF GFI  
Sbjct: 277 FVSDYLWVIAMLMTSPLLVTVGMSLSIPLALFFDILLKGHYLNFSLILGSLLVFAGFIVV 334

BLAST of Spg017623 vs. ExPASy Swiss-Prot
Match: A6QL92 (Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 8.5e-22
Identity = 80/246 (32.52%), Postives = 123/246 (50.00%), Query Frame = 0

Query: 60  KLDTKEVALLAICIGPLWFTSEYLTSCALAHTSVATTTILFSTSGLFTLVIDGFFKKSIN 119
           KL   +VA ++     +WF + +    AL+ T VA   IL STSGLFTL++   F  +  
Sbjct: 235 KLTATQVAKISFFFCFVWFLANFSYQEALSDTQVAIVNILSSTSGLFTLILAAMFPSNSG 294

Query: 120 G----VNVISVIISMAGVAMTTTAKTWARDEPTLNEKHSLVGDAFALLSALTDGLYYVLL 179
                  +++VI+S+ GV +     + +   P  N     +G  ++L+ A+   +Y V++
Sbjct: 295 DRFTLSKLLAVILSIGGVVLVNL--SGSEKSPGRN----TIGSIWSLVGAMLYAVYIVMI 354

Query: 180 NKYAGENGQKLDLQKFLGFVGLFVFSGLWWLVWPLTALGIEPKFALPKSAKLMEVIVANC 239
            +   +   KLD+  F GFVGLF    LW   + L   G E  F  P    LM  IV N 
Sbjct: 355 KRKV-DREDKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEFPNKVVLM-CIVING 414

Query: 240 FLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLTDMLIHGRHYSLLYILGSIQVFVG 299
            +G  ++++ W  G   TS L+  L  SLTIPL+++ DM +    +S L+  G+I VF  
Sbjct: 415 LIGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGAIPVFFS 471

Query: 300 FIAANL 302
           F  A L
Sbjct: 475 FFIATL 471

BLAST of Spg017623 vs. ExPASy Swiss-Prot
Match: Q8WV83 (Solute carrier family 35 member F5 OS=Homo sapiens OX=9606 GN=SLC35F5 PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.5e-21
Identity = 81/252 (32.14%), Postives = 123/252 (48.81%), Query Frame = 0

Query: 54  IPKKERKLDTKEVALLAICIGPLWFTSEYLTSCALAHTSVATTTILFSTSGLFTLVIDGF 113
           I K   KL   +VA ++     +WF +      AL+ T VA   IL STSGLFTL++   
Sbjct: 228 ILKTVGKLTATQVAKISFFFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAV 287

Query: 114 FKKSING----VNVISVIISMAGVAMTTTAKTWARDEPTLNEKHSLVGDAFALLSALTDG 173
           F  +         +++VI+S+ GV +   A     ++P        VG  ++L  A+   
Sbjct: 288 FPSNSGDRFTLSKLLAVILSIGGVVLVNLA---GSEKPA---GRDTVGSIWSLAGAMLYA 347

Query: 174 LYYVLLNKYAGENGQKLDLQKFLGFVGLFVFSGLWWLVWPLTALGIEPKFALPKSAKLME 233
           +Y V++ +   +   KLD+  F GFVGLF    LW   + L   G E  F  P    LM 
Sbjct: 348 VYIVMIKRKV-DREDKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEFPNKVVLMC 407

Query: 234 VIVANCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLTDMLIHGRHYSLLYILGS 293
           +I+ N  +G  ++++ W  G   TS L+  L  SLTIPL+++ DM +    +S L+  G+
Sbjct: 408 III-NGLIGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGA 467

Query: 294 IQVFVGFIAANL 302
           I VF  F    L
Sbjct: 468 IPVFFSFFIVTL 470

BLAST of Spg017623 vs. ExPASy TrEMBL
Match: A0A6J1C0M9 (uncharacterized transporter C405.03c-like OS=Momordica charantia OX=3673 GN=LOC111006993 PE=4 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 8.0e-100
Identity = 193/325 (59.38%), Postives = 248/325 (76.31%), Query Frame = 0

Query: 2   EVQISKEEVESSKTCNDNKRQRSLTND----QCVVDIEARE------NGNDIICKCEGEV 61
           +V   K    SS   N+N    S   D     CVV I+  E        N   C+CE + 
Sbjct: 83  QVMEEKSNNPSSPNNNNNNNNISEVGDGSEVHCVVQIQCEEEVVSVDKDNYGGCECENQE 142

Query: 62  IDIPKKERKLDTKEVALLAICIGPLWFTSEYLTSCALAHTSVATTTILFSTSGLFTLVID 121
           + +  K ++    + A+LA+ IGP+WF SEY T+ ALA TSVATTTILFSTSGLFTL+ID
Sbjct: 143 VAMALKTKE---TKFAVLALTIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIID 202

Query: 122 GFF-KKSINGVNVISVIISMAGVAMTTTAKTWARDEP--TLNE--KHSLVGDAFALLSAL 181
            F  ++S+  VN+++V++S+AGVAMTT  KTWA+D+P  T +E  KHS VGDAFALLSAL
Sbjct: 203 AFLERQSLTIVNIVAVVVSLAGVAMTTIGKTWAKDQPHSTSSEHGKHSFVGDAFALLSAL 262

Query: 182 TDGLYYVLLNKYAGENGQKLDLQKFLGFVGLFVFSGLWWLVWPLTALGIEPKFALPKSAK 241
           TDGLYYVLL KYAGE G+K+D+QKFLG+VGLF  + LWWL+WPL A+GIEPKFA+P+S K
Sbjct: 263 TDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFAIPRSTK 322

Query: 242 LMEVIVANCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLTDMLIHGRHYSLLYI 301
           + E+++ANCF+GNF++DYFWA+G +WTSPLVAALG+SLTIPLAML DM++HGRHYSL+YI
Sbjct: 323 VAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYI 382

Query: 302 LGSIQVFVGFIAANLSDWLTPKLRL 312
            GS+QVF+GF+ ANLSDW++PKL+L
Sbjct: 383 FGSLQVFLGFMIANLSDWISPKLKL 404

BLAST of Spg017623 vs. ExPASy TrEMBL
Match: A0A6J1EHX2 (uncharacterized transporter C405.03c-like OS=Cucurbita moschata OX=3662 GN=LOC111432715 PE=4 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 1.4e-99
Identity = 187/318 (58.81%), Postives = 244/318 (76.73%), Query Frame = 0

Query: 8   EEVESSKTCNDNKRQRSLTNDQ-----CVVDIEAREN-----GNDIICKCEGEVIDIPKK 67
           +E +++   N+N    +++  +     CVV++E  E       N+  C+CE E      +
Sbjct: 80  QEQDNNNNNNNNNNNNNISEVENGDVHCVVNVECEEQEKGSASNNGRCECEAE---NGTQ 139

Query: 68  ERKLDTKEVALLAICIGPLWFTSEYLTSCALAHTSVATTTILFSTSGLFTLVIDGFF-KK 127
           E    TK++A+LA+ IGP+WF SEY T+ ALA TSVATTTILFSTSGLFTL++D  F ++
Sbjct: 140 ETSFSTKQMAVLALAIGPIWFVSEYFTNAALAKTSVATTTILFSTSGLFTLILDACFERQ 199

Query: 128 SINGVNVISVIISMAGVAMTTTAKTWARDEPTL---NEKHSLVGDAFALLSALTDGLYYV 187
           S++ VNV++V +SMAGVAMTT  KTWARDE      +EKHS +GD FALLSA TDGLYYV
Sbjct: 200 SLSIVNVVAVFVSMAGVAMTTVGKTWARDEAQSSFGHEKHSYIGDVFALLSAFTDGLYYV 259

Query: 188 LLNKYAGENGQKLDLQKFLGFVGLFVFSGLWWLVWPLTALGIEPKFALPKSAKLMEVIVA 247
           LL KYAGE G+K+D+QKFLG+VGLF  + LWWL+WPL A+GIEPKF LP+S K+ E+++A
Sbjct: 260 LLKKYAGEEGEKVDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFGLPQSTKVAEIVLA 319

Query: 248 NCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLTDMLIHGRHYSLLYILGSIQVF 307
           NCF+ NFV+DY WA+G +WTSPLVAALG+SLTIPLAM+ DML+HGRHYSL+YI GS+QVF
Sbjct: 320 NCFVSNFVSDYLWAMGVVWTSPLVAALGASLTIPLAMVGDMLLHGRHYSLVYIFGSLQVF 379

Query: 308 VGFIAANLSDWLTPKLRL 312
           +GF+ AN SDW++PKL+L
Sbjct: 380 LGFMIANFSDWISPKLKL 394

BLAST of Spg017623 vs. ExPASy TrEMBL
Match: A0A5D3C4N7 (Putative transporter-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00180 PE=4 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.8e-99
Identity = 188/300 (62.67%), Postives = 233/300 (77.67%), Query Frame = 0

Query: 17  NDNKRQRSLTNDQCVVDIEARENGNDIICKCEGEVIDIPKKERKLDTKEVALLAICIGPL 76
           N+N    S  N  C  ++   EN    I K E         E K  TK++A+LA+ IGP+
Sbjct: 121 NNNSNNSSNNNGGC--ELREAENMEMRILKTE---------EAKFSTKQIAVLALTIGPI 180

Query: 77  WFTSEYLTSCALAHTSVATTTILFSTSGLFTLVIDGFF-KKSINGVNVISVIISMAGVAM 136
           WF SEY T+ ALA TSVATTTILFSTSGLFTL++D    ++S+  VNV++V++SMAGVAM
Sbjct: 181 WFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACLERQSLTIVNVVAVVVSMAGVAM 240

Query: 137 TTTAKTWARDEPTLNE----KHSLVGDAFALLSALTDGLYYVLLNKYAGENGQKLDLQKF 196
           TT  KTWA+DEP  +     KHS VGDAFALLSALTDGLYYVLL KYAGE G+K+D+QKF
Sbjct: 241 TTVGKTWAKDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKVDMQKF 300

Query: 197 LGFVGLFVFSGLWWLVWPLTALGIEPKFALPKSAKLMEVIVANCFLGNFVADYFWALGAI 256
           LG+VGLF  + LWWL+WPL A+GIEPKF +P+S K+ EV++ANCF+ NFV+DYFWA+G +
Sbjct: 301 LGYVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFVANFVSDYFWAMGVV 360

Query: 257 WTSPLVAALGSSLTIPLAMLTDMLIHGRHYSLLYILGSIQVFVGFIAANLSDWLTPKLRL 312
           WTSPLVAALG+SLTIPLAM+ DM++HGRHYSL+YI GSIQVF+GFI AN SDW++PKL+L
Sbjct: 361 WTSPLVAALGASLTIPLAMIGDMVLHGRHYSLVYIFGSIQVFLGFIIANFSDWISPKLKL 409

BLAST of Spg017623 vs. ExPASy TrEMBL
Match: A0A6J1L0H0 (uncharacterized vacuolar membrane protein YML018C-like OS=Cucurbita maxima OX=3661 GN=LOC111499921 PE=4 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 2.3e-99
Identity = 188/325 (57.85%), Postives = 246/325 (75.69%), Query Frame = 0

Query: 2   EVQISKEEVESSKTCNDNKRQRSLTND------QCVVDIEAREN-----GNDIICKCEGE 61
           E+Q   ++  ++   N+N    +  ++       CVV+IE  E       N+  C+CE E
Sbjct: 78  EIQEQDKDNNNNNINNNNNNNNNNISEVENGDVLCVVNIECEEQEKGSVSNNDRCECEAE 137

Query: 62  VIDIPKKERKLDTKEVALLAICIGPLWFTSEYLTSCALAHTSVATTTILFSTSGLFTLVI 121
                 +E    TK++A+LA+ IGP+WF SEY T+ ALA TSVATTTILFSTSGLFTL++
Sbjct: 138 ---NGTQETSFSTKKIAVLALAIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIL 197

Query: 122 DGFF-KKSINGVNVISVIISMAGVAMTTTAKTWARDE---PTLNEKHSLVGDAFALLSAL 181
           D  F ++S++ VNV++V +SMAGVAMTT  KTWARDE    + +EKHS VGD FALLSA 
Sbjct: 198 DACFERQSLSIVNVVAVFVSMAGVAMTTIGKTWARDEAQSSSRHEKHSYVGDVFALLSAF 257

Query: 182 TDGLYYVLLNKYAGENGQKLDLQKFLGFVGLFVFSGLWWLVWPLTALGIEPKFALPKSAK 241
           TDGLYYVLL KYAGE G+K+D+QKFLG+VGLF  + LWWL+WPL A+GIEPKF +P+S K
Sbjct: 258 TDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTLTTLWWLIWPLKAIGIEPKFGIPQSTK 317

Query: 242 LMEVIVANCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLTDMLIHGRHYSLLYI 301
           + E+++ANCF+ NFV+DY WA+G +WTSPL+AALG+SLTIPLAM+ DML+HGRHYSL+YI
Sbjct: 318 VAEIVIANCFVSNFVSDYLWAMGVVWTSPLIAALGASLTIPLAMVGDMLLHGRHYSLVYI 377

Query: 302 LGSIQVFVGFIAANLSDWLTPKLRL 312
            GS+QVF+GF+ AN SDW++PKL+L
Sbjct: 378 FGSLQVFLGFMIANFSDWISPKLKL 399

BLAST of Spg017623 vs. ExPASy TrEMBL
Match: A0A5A7U493 (Putative transporter-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00790 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 2.6e-98
Identity = 190/322 (59.01%), Postives = 241/322 (74.84%), Query Frame = 0

Query: 7   KEEVESSKTCNDNKRQRSLTND--QCVVDI---------EARENGNDIICKCEGEVIDIP 66
           +E    S   N+N     + N   Q VV+I          +  NG   +C+ E   + I 
Sbjct: 88  EERTNQSSNNNNNTTSDEVENRDVQYVVNINDNNNNSNNSSNNNGGCELCEAENMEMRIL 147

Query: 67  K-KERKLDTKEVALLAICIGPLWFTSEYLTSCALAHTSVATTTILFSTSGLFTLVIDGFF 126
           K +E K  TK++A+LA+ IGP+WF SEY T+ ALA TSVATTTILFSTSGLFTL++D   
Sbjct: 148 KTEEAKFSTKQIAVLALTIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACL 207

Query: 127 -KKSINGVNVISVIISMAGVAMTTTAKTWARDEPTLNE----KHSLVGDAFALLSALTDG 186
            ++S+  VN ++V++SMAGV MTT  KTWA+DEP  +     KHS VGDAFALLSALTDG
Sbjct: 208 ERQSLTIVNAVAVVVSMAGVVMTTVGKTWAKDEPQSSSYGHGKHSFVGDAFALLSALTDG 267

Query: 187 LYYVLLNKYAGENGQKLDLQKFLGFVGLFVFSGLWWLVWPLTALGIEPKFALPKSAKLME 246
           LYYVLL KYAGE G+K+D+QKFLG+VGLF  + LWWL+WPL A+GIEPKF +P+S K+ E
Sbjct: 268 LYYVLLKKYAGEEGEKVDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAE 327

Query: 247 VIVANCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLTDMLIHGRHYSLLYILGS 306
           V++ANCF+ NFV+DYFWA+G +WTSPLVAALG+SLTIPLAM+ DM++H RHYSL+YI GS
Sbjct: 328 VVLANCFVANFVSDYFWAMGVVWTSPLVAALGASLTIPLAMIGDMVLHDRHYSLVYIFGS 387

Query: 307 IQVFVGFIAANLSDWLTPKLRL 312
           IQVF+GFI AN SDW++PKL+L
Sbjct: 388 IQVFLGFIIANFSDWISPKLKL 409

BLAST of Spg017623 vs. TAIR 10
Match: AT4G32140.1 (EamA-like transporter family )

HSP 1 Score: 301.6 bits (771), Expect = 7.5e-82
Identity = 154/279 (55.20%), Postives = 206/279 (73.84%), Query Frame = 0

Query: 38  ENGNDIICKCEGEVIDIPKKERKLDTKEVALLAICIGPLWFTSEYLTSCALAHTSVATTT 97
           E+   +I K   EV  + K+ +++ TK++A+  + + P+WF +EYL++ ALA TSVA+TT
Sbjct: 117 EDERPLIGKVREEVQTL-KQRKEITTKQIAMYGLYLAPIWFVTEYLSNAALARTSVASTT 176

Query: 98  ILFSTSGLFTLVIDGFF-KKSINGVNVISVIISMAGVAMTTTAKTWARDE----PTLNEK 157
           +L STSGLFTL I  F  + ++N   V++V +SMAGV MTT  KTWA DE     +LN +
Sbjct: 177 VLSSTSGLFTLFIGVFLGQDTLNLSKVVAVFVSMAGVVMTTLGKTWASDESQLNSSLNGE 236

Query: 158 HSLVGDAFALLSALTDGLYYVLLNKYAGENGQKLDLQKFLGFVGLFVFSGLWWLVWPLTA 217
            SL+GD F LLSA++ GL+ VLL K+AGE G+ +D+QK  G++GLF    LWWLVWPLTA
Sbjct: 237 RSLMGDLFGLLSAVSYGLFTVLLKKFAGEEGEGVDVQKLFGYIGLFTLVALWWLVWPLTA 296

Query: 218 LGIEPKFALPKSAKLMEVIVANCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLT 277
           LGIEPKF +P S K+ EV++AN F+G+ ++DYFWAL  +WT+PLVA LG SLTIPLAM+ 
Sbjct: 297 LGIEPKFTIPHSVKVDEVVLANGFIGSVLSDYFWALSVVWTTPLVATLGMSLTIPLAMVA 356

Query: 278 DMLIHGRHYSLLYILGSIQVFVGFIAANLSDWLTPKLRL 312
           DM+IHGRHYS +YILGS QVF GF+ AN+SD  + KL L
Sbjct: 357 DMMIHGRHYSAIYILGSTQVFAGFVIANISDLFSKKLGL 394

BLAST of Spg017623 vs. TAIR 10
Match: AT3G07080.1 (EamA-like transporter family )

HSP 1 Score: 87.0 bits (214), Expect = 2.9e-17
Identity = 85/323 (26.32%), Postives = 147/323 (45.51%), Query Frame = 0

Query: 1   MEVQISKEEVESSKTCNDNKRQRSLTNDQCVVDIEARENGNDIICKCEGEVIDIPK---- 60
           ME+  S++ V   K  +  K   + ++   V + E  E GN I    E   ++I      
Sbjct: 81  MELVESEKAVLLGKDVSGVKSDATESSGLVVREQEISEGGNGIESGLENIELEIDASVNV 140

Query: 61  -------KERKLDTK------EVALLAICIGPLWFTSEYLTSCALAHTSVATTTILFSTS 120
                    + LD K       VA +++ I P WF ++   + +L +T+V + TIL S S
Sbjct: 141 SDGASGVSNKGLDEKGRWTRMRVAKVSLVICPFWFLAQLTFNVSLKYTTVTSNTILSSAS 200

Query: 121 GLFTLVID-GFFKKSINGVNVISVIISMAGVAMTTTAKTWARDEPTLNEKHSLVGDAFAL 180
            LFT ++   F  +    + + SV++ M+G  + +   + ++       K+ L+GD  +L
Sbjct: 201 SLFTFLVSLIFLGEKFTWLKLFSVLLCMSGTIIVSMGDSESKSNAV--AKNPLLGDILSL 260

Query: 181 LSALTDGLYYVLLNKYA---GENGQKLDLQKFLGFVGLFVFSGLWWLVWPLTALGIEPKF 240
           +SA    +Y  L+ K      E   ++ + +FLGF+GLF F   +  + P   L    + 
Sbjct: 261 VSAALYAVYITLIRKKLPDDDERNGRVSMAQFLGFLGLFNF---FIFLPPALILNFTKRE 320

Query: 241 AL-PKSAKLMEVIVANCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLTDMLIHG 300
                + K   ++V    L N ++DY WA   + T+  VA  G ++ +PLA + D L  G
Sbjct: 321 RFNALTLKQFGLVVGKGLLDNVLSDYLWAKAVLLTTTTVATAGLTIQVPLAAIVDSL-SG 380

Query: 301 RHYSLLYILGSIQVFVGFIAANL 302
              S    +G+  V VGF   N+
Sbjct: 381 NKPSFTDYIGAAAVMVGFAGINI 397