Spg017533 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg017533 vs. NCBI nr
Match: KAG6586114.1 (Protein SMAX1-LIKE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1399.0 bits (3620), Expect = 0.0e+00
Identity = 781/1033 (75.61%), Postives = 840/1033 (81.32%), Query Frame = 0

Query: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSK---PTLLRRACL 60
           MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLS+    PTLLRRACL
Sbjct: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSNNSKPPTLLRRACL 60

Query: 61  KSHPPHPLHSRALDLCFNVALNRLPTSPPLLHS-PSLSNALIAALKRAQAHQRR------ 120
           KSHPPHPL SRAL+LCFNVALNRLP+SPPLLHS PSLSNALIAALKRAQAHQRR      
Sbjct: 61  KSHPPHPLQSRALELCFNVALNRLPSSPPLLHSPPSLSNALIAALKRAQAHQRRGSSSSS 120

Query: 121 --LDHPPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIEEFN 180
             LDH          HPLL++KV L HLIISILDDPSVSRVMREAGFSSTAVK NIEE+N
Sbjct: 121 SSLDH---HHQQQQQHPLLTVKVELQHLIISILDDPSVSRVMREAGFSSTAVKTNIEEYN 180

Query: 181 ----------SATTPISS-----------PLFFSSSSPSLPRNHSSSDAAANFVFELFLR 240
                     S  +PISS           P FFSSSSP  P     +  A   VFE FL 
Sbjct: 181 DNNNNNTIFISPPSPISSHFFSPQTNTYTPFFFSSSSPPPP---PPTTDATKLVFEAFLG 240

Query: 241 RSN----SNVVVVGDSVGVTEGVVFEVMRKLKTGEVPHQMKGVKFVELLPPFMGISSSSS 300
           ++N    +NVVVVGDSVG+TEGVVFEV+RK+K GEVP  MKGVKFVE LP   G SS   
Sbjct: 241 KNNNNLRTNVVVVGDSVGLTEGVVFEVIRKVKMGEVPEVMKGVKFVEFLPLMKGSSS--- 300

Query: 301 PSPSSSELRRKLRECCCGSDDADNMGVVVYVGDLKWIVEGSN-EEVDGLVGEIERLLIKG 360
                     KL E     D+ D  GV+VYVGDLKWIVEG N +E++ LVGEIER L KG
Sbjct: 301 ---------LKLGEYV--KDNGDG-GVLVYVGDLKWIVEGGNSDEIERLVGEIERSL-KG 360

Query: 361 DSSFNGDGNTFGSNKVKIWLIAVASYQVYMRCQMRQPALETQWALHALPLPSSGLALTLH 420
           D   N + N    +K KIW++ +ASYQ+YMRCQMRQPALETQW+LHA+P+PSSGL LTLH
Sbjct: 361 D-FLNANNN---GSKAKIWVMGMASYQIYMRCQMRQPALETQWSLHAVPVPSSGLGLTLH 420

Query: 421 ASSVYDSRLSFFSQPMETKPFLAKEEQEKLTCCAECTSNFENEAQHLKSFQSNQVPSWLQ 480
            SSVYDSR SFFSQ METK F+AKEE EKLTCCAECTSNFENE QHLKSFQS QVPSWLQ
Sbjct: 421 TSSVYDSRPSFFSQTMETKQFIAKEEHEKLTCCAECTSNFENEVQHLKSFQSKQVPSWLQ 480

Query: 481 QYNANQSHSKDEYVELRRKWNRFCSSLHRDGSVQSFMAGKSFSYSSSYPWWPKFHESNSI 540
           QYN NQSHSKDE+VELR+KWNRFCSSLHRDGS QS M GKSFSY SSYPWWPKF ESNSI
Sbjct: 481 QYNVNQSHSKDEFVELRKKWNRFCSSLHRDGSAQSLM-GKSFSYCSSYPWWPKFDESNSI 540

Query: 541 SFTDHQTTKPLQSSSFVPRFRRQQSCTTIEFDFGNATTKHDQGRE-PSLNSLKHMVGKEV 600
           SFTD+QT KPLQSS+ VPRFRRQQSCTTIEFDFGNATTK  Q RE PSLNSLKHMVGKEV
Sbjct: 541 SFTDNQTPKPLQSSNLVPRFRRQQSCTTIEFDFGNATTKQSQDRESPSLNSLKHMVGKEV 600

Query: 601 KITLALGSPLFCDSSADSMEMESERNSQRGEILKVLQENVPWQSESLPCVAEAVISAKKN 660
           KITLALG+PLF DSSA+SMEMESER ++RGEILKVLQENVPWQSESLP +AEAVISAKKN
Sbjct: 601 KITLALGNPLFYDSSAESMEMESERKTERGEILKVLQENVPWQSESLPSIAEAVISAKKN 660

Query: 661 EKSIEWILIEGNDFIGKRKLALAIAESAFGSVDSLLNLNAKSEEMGISRCEMVEKALKSN 720
           EK I+WIL+EGNDFIGKRK+ALAIAES FGS++  LNLNAKSEEMGISR EMVEKALKS 
Sbjct: 661 EKRIQWILMEGNDFIGKRKMALAIAESVFGSIEFFLNLNAKSEEMGISRSEMVEKALKST 720

Query: 721 RELVILVEDVDMADSQFMKFLEDGFESGKLGEAEEETIEKVIFVLTKDDSSDKKKNRASS 780
           RELV+LVEDV+MADSQFMKFLEDGFESGK GE +EE IEK+IFVLTKDDSSDKKKNR SS
Sbjct: 721 RELVLLVEDVEMADSQFMKFLEDGFESGKFGEVKEERIEKLIFVLTKDDSSDKKKNRDSS 780

Query: 781 SSVIEMILKIDARAKPNSDHKRKAEREFENKSKKQRINTYEYENQNNRKEFSRQSIINNI 840
           SSVIEM L+IDAR K    HKRKAERE ENKSKK RIN        NR    + SI NN 
Sbjct: 781 SSVIEMALEIDAREK----HKRKAEREIENKSKKARIN-------KNR----QSSINNNT 840

Query: 841 LDLNLKASNEE----EEEDEETDQKLPNGQISPISSDLTRETTIYDLKPANGFLESISNR 900
           +DLN KA+NEE    E++D ET+Q LPNGQISPISSDLTRETT+YD K ANGFLESISN 
Sbjct: 841 IDLNQKAANEEDQKQEQDDAETNQTLPNGQISPISSDLTRETTMYDRKAANGFLESISNL 900

Query: 901 FILNRKPSQESEITEQLRGKMKGAYKENCKKGNWNWDWDWDWNWDSRFRVEEGVLEGILE 960
           FILN K +QESEI EQLR  M GAY+ENCKK  W+WDWDWDWNWD RFRVEEGVLEGILE
Sbjct: 901 FILNSKSTQESEIREQLRRIMTGAYEENCKKWKWDWDWDWDWNWDCRFRVEEGVLEGILE 960

Query: 961 GFGSFSNKVFEKWVKEIFQTSLEGGRYGGKGEGGIDIRLCLDQKHILEEE-EEEEDGYMG 990
           GFGSFSNKVFEKWVKEIFQTSLEGGRYGGK EGGIDIRLCLDQKHILEEE EE E+GYMG
Sbjct: 961 GFGSFSNKVFEKWVKEIFQTSLEGGRYGGKQEGGIDIRLCLDQKHILEEEHEEHEEGYMG 991

BLAST of Spg017533 vs. NCBI nr
Match: XP_023537625.1 (protein SMAX1-LIKE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1397.5 bits (3616), Expect = 0.0e+00
Identity = 779/1032 (75.48%), Postives = 838/1032 (81.20%), Query Frame = 0

Query: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSK---PTLLRRACL 60
           MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLS+    PTLLRRACL
Sbjct: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSNNSKPPTLLRRACL 60

Query: 61  KSHPPHPLHSRALDLCFNVALNRLPTSPPLLHS-PSLSNALIAALKRAQAHQRR------ 120
           KSHPPHPL SRAL+LCFNVALNRLP+SPPLLHS PSLSNALIAALKRAQAHQRR      
Sbjct: 61  KSHPPHPLQSRALELCFNVALNRLPSSPPLLHSPPSLSNALIAALKRAQAHQRRGSSSSS 120

Query: 121 -LDHPPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIEEFN- 180
            LDH          HPLL++KV L HLIISILDDPSVSRVMREAGFSSTAVK NIEE+N 
Sbjct: 121 SLDH---HHQQQQQHPLLTVKVELQHLIISILDDPSVSRVMREAGFSSTAVKTNIEEYND 180

Query: 181 ---------SATTPISS-----------PLFFSSSSPSLPRNHSSSDAAANFVFELFLRR 240
                    S  +PISS           P FFSSS P  P     +  A   VFE FL +
Sbjct: 181 NNNNNTIFISPPSPISSHFFSPQTNTYTPFFFSSSPPPPP-----TTDATKLVFEAFLGK 240

Query: 241 SN----SNVVVVGDSVGVTEGVVFEVMRKLKTGEVPHQMKGVKFVELLPPFMGISSSSSP 300
           +N    +NVVVVGDSVG+TEGVVFEVMRK+K GEVP  MKGVKFVE LP   G SS    
Sbjct: 241 NNNNLRTNVVVVGDSVGLTEGVVFEVMRKVKMGEVPEVMKGVKFVEFLPLMKGSSS---- 300

Query: 301 SPSSSELRRKLRECCCGSDDADNMGVVVYVGDLKWIVEGSN-EEVDGLVGEIERLLIKGD 360
                    KL E     D+ D  GV+VYVGDLKWIVEG N +E++ LVGEIERLL KGD
Sbjct: 301 --------LKLGEYV--KDNGDG-GVLVYVGDLKWIVEGGNSDEIERLVGEIERLL-KGD 360

Query: 361 SSFNGDGNTFGSNKVKIWLIAVASYQVYMRCQMRQPALETQWALHALPLPSSGLALTLHA 420
              N + N    +K KIW++ +ASYQ+YMRCQMRQPALETQW+LHA+P+PSSGL LTLH 
Sbjct: 361 -FLNTNNN---GSKAKIWVMGMASYQIYMRCQMRQPALETQWSLHAVPVPSSGLGLTLHT 420

Query: 421 SSVYDSRLSFFSQPMETKPFLAKEEQEKLTCCAECTSNFENEAQHLKSFQSNQVPSWLQQ 480
           SSVYDSR SFFSQ  ETK F+AKEE EKLTCCAECTSNFENE QHLKSFQS QVPSWLQQ
Sbjct: 421 SSVYDSRPSFFSQTRETKQFIAKEEHEKLTCCAECTSNFENEVQHLKSFQSKQVPSWLQQ 480

Query: 481 YNANQSHSKDEYVELRRKWNRFCSSLHRDGSVQSFMAGKSFSYSSSYPWWPKFHESNSIS 540
           YN NQSHSKDE+VELR+KWNRFCSSLHRDGS QS M GKSFSY SSYPWWPKF ESNSIS
Sbjct: 481 YNVNQSHSKDEFVELRKKWNRFCSSLHRDGSAQSLM-GKSFSYCSSYPWWPKFDESNSIS 540

Query: 541 FTDHQTTKPLQSSSFVPRFRRQQSCTTIEFDFGNATTKHDQGRE-PSLNSLKHMVGKEVK 600
           FTD+QT KPLQSS+ VPRFRRQQSCTTIEFDFGNATTK  Q RE PSLNSLKHMVGKEVK
Sbjct: 541 FTDNQTPKPLQSSNLVPRFRRQQSCTTIEFDFGNATTKQSQDRESPSLNSLKHMVGKEVK 600

Query: 601 ITLALGSPLFCDSSADSMEMESERNSQRGEILKVLQENVPWQSESLPCVAEAVISAKKNE 660
           ITLALG+PLF DSSA+SMEMESER ++RGEILKVLQENVPWQSESLP +AEAVISAKKNE
Sbjct: 601 ITLALGNPLFYDSSAESMEMESERKTERGEILKVLQENVPWQSESLPSIAEAVISAKKNE 660

Query: 661 KSIEWILIEGNDFIGKRKLALAIAESAFGSVDSLLNLNAKSEEMGISRCEMVEKALKSNR 720
           K I+WIL+EGNDFIGKRK+ALAIAES FGS++  LNLNAKSEEMGISR EMVEKALKS R
Sbjct: 661 KRIQWILMEGNDFIGKRKMALAIAESVFGSIEFFLNLNAKSEEMGISRSEMVEKALKSTR 720

Query: 721 ELVILVEDVDMADSQFMKFLEDGFESGKLGEAEEETIEKVIFVLTKDDSSDKKKNRASSS 780
           ELVILVEDV+MADSQFMKFLEDGFESGK GE +EE IEK+IFVLTKDDSSDKKKNR SSS
Sbjct: 721 ELVILVEDVEMADSQFMKFLEDGFESGKFGEVKEERIEKLIFVLTKDDSSDKKKNRDSSS 780

Query: 781 SVIEMILKIDARAKPNSDHKRKAEREFENKSKKQRINTYEYENQNNRKEFSRQSIINNIL 840
           SVIEM L+IDAR K    HKRKAERE ENKSKK RIN     + NN          NN +
Sbjct: 781 SVIEMTLEIDAREK----HKRKAEREIENKSKKARINKNRQSSINN----------NNTI 840

Query: 841 DLNLKASNEE----EEEDEETDQKLPNGQISPISSDLTRETTIYDLKPANGFLESISNRF 900
           DLNLKA+NEE    E++D ET+Q LPNGQISPISSDLTRETT+Y+LKPANGF+ESISNRF
Sbjct: 841 DLNLKAANEEDQKQEQDDAETNQTLPNGQISPISSDLTRETTMYNLKPANGFMESISNRF 900

Query: 901 ILNRKPSQESEITEQLRGKMKGAYKENCKKGNWNWDWDWDWNWDSRFRVEEGVLEGILEG 960
           +L  K +QESEI EQLR  M GAY+ENCKK  W WDWDWDWNWD RFRVEEGVLEGILEG
Sbjct: 901 LLKSKSTQESEIREQLRRIMTGAYEENCKK--WKWDWDWDWNWDCRFRVEEGVLEGILEG 960

Query: 961 FGSFSNKVFEKWVKEIFQTSLEGGRYGGKGEGGIDIRLCLDQKHILEEE-EEEEDGYMGS 990
           FGSFSNKVFEKWV EIFQTSLEGGRYGGK EGGIDIRLCLDQKHILEEE EE E+GYMGS
Sbjct: 961 FGSFSNKVFEKWVNEIFQTSLEGGRYGGKQEGGIDIRLCLDQKHILEEEHEEHEEGYMGS 987

BLAST of Spg017533 vs. NCBI nr
Match: XP_022937893.1 (protein SMAX1-LIKE 4-like [Cucurbita moschata])

HSP 1 Score: 1392.5 bits (3603), Expect = 0.0e+00
Identity = 779/1036 (75.19%), Postives = 838/1036 (80.89%), Query Frame = 0

Query: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSK---PTLLRRACL 60
           MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLS+    PTLLRRACL
Sbjct: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSNNSKPPTLLRRACL 60

Query: 61  KSHPPHPLHSRALDLCFNVALNRLPTSPPLLHS-PSLSNALIAALKRAQAHQRR------ 120
           KSHPPHPL SRAL+LCFNVALNRLP+SPPLLHS PSLSNALIAALKRAQAHQRR      
Sbjct: 61  KSHPPHPLQSRALELCFNVALNRLPSSPPLLHSPPSLSNALIAALKRAQAHQRRGSSSSS 120

Query: 121 --LDHPPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIEEFN 180
             LDH          HPLL++KV L HLIISILDDPSVSRVMREAGFSSTAVK NIEE+N
Sbjct: 121 SSLDH---HHQQQQQHPLLTVKVELQHLIISILDDPSVSRVMREAGFSSTAVKTNIEEYN 180

Query: 181 ----------SATTPISS-----------PLFFSSSSPSLPRNHSSSDAAANFVFELFLR 240
                     S  +PISS           P FFSSSSP  P     +  A   VFE FL 
Sbjct: 181 DNNNNNTIFISPPSPISSHFFSPQTNTYTPFFFSSSSPPPP---PPTTDATKLVFEAFLG 240

Query: 241 RSN----SNVVVVGDSVGVTEGVVFEVMRKLKTGEVPHQMKGVKFVELLPPFMGISSSSS 300
           ++N    +NVVVVGDSVG+TEGVVFEV+RK+K GEVP  MKGVKFVE LP   G SS   
Sbjct: 241 KNNNNLRTNVVVVGDSVGLTEGVVFEVIRKVKMGEVPEVMKGVKFVEFLPLMKGSSS--- 300

Query: 301 PSPSSSELRRKLRECCCGSDDADNMGVVVYVGDLKWIVEGSN-EEVDGLVGEIERLLIKG 360
                     KL E     D+ D  GV+VYVGDLKWIVEG N +E++ LVGEIER L KG
Sbjct: 301 ---------LKLGEYV--KDNGDG-GVLVYVGDLKWIVEGGNSDEIERLVGEIERSL-KG 360

Query: 361 DSSFNGDGNTFGSNKVKIWLIAVASYQVYMRCQMRQPALETQWALHALPLPSSGLALTLH 420
           D   N + N    +K KIW++ +ASYQ+YMRCQMRQPALETQW+LHA+P+PSSGL LTLH
Sbjct: 361 D-FLNANNN---GSKAKIWVMGMASYQIYMRCQMRQPALETQWSLHAVPVPSSGLGLTLH 420

Query: 421 ASSVYDSRLSFFSQPMETKPFLAKEEQEKLTCCAECTSNFENEAQHLKSFQSNQVPSWLQ 480
            SSVYDSR SFFSQ METK F+AKEE EKLTCCAECTSNFENE QHLKSFQS QVPSWLQ
Sbjct: 421 TSSVYDSRPSFFSQTMETKQFIAKEEHEKLTCCAECTSNFENEVQHLKSFQSKQVPSWLQ 480

Query: 481 QYNANQSHSKDEYVELRRKWNRFCSSLHRDGSVQSFMAGKSFSYSSSYPWWPKFHESNSI 540
           QYN NQSHSKDE+VELR+KWNRFCSSLHRDGS QS M GKSFSY SSYPWWPKF ESNSI
Sbjct: 481 QYNVNQSHSKDEFVELRKKWNRFCSSLHRDGSAQSLM-GKSFSYCSSYPWWPKFDESNSI 540

Query: 541 SFTDHQTTKPLQSSSFVPRFRRQQSCTTIEFDFGNATTKHDQGRE-PSLNSLKHMVGKEV 600
           SFTD+QT KPLQSS+ VPRFRRQQSCTTIEFDFGNATTK  Q RE PSLNSLKHMVGKEV
Sbjct: 541 SFTDNQTPKPLQSSNLVPRFRRQQSCTTIEFDFGNATTKQSQDRESPSLNSLKHMVGKEV 600

Query: 601 KITLALGSPLFCDSSADSMEMESERNSQRGEILKVLQENVPWQSESLPCVAEAVISAKKN 660
           KITLALG+PLF DSSA+SMEMESER ++RGEILKVLQENVPWQSESL  +AE VISAKKN
Sbjct: 601 KITLALGNPLFYDSSAESMEMESERKTERGEILKVLQENVPWQSESLHSIAETVISAKKN 660

Query: 661 EKSIEWILIEGNDFIGKRKLALAIAESAFGSVDSLLNLNAKSEEMGISRCEMVEKALKSN 720
           EK I+WIL+EGNDFIGKRK+ALAIAE  FGS++  LNLNAKSEEMGISR E+VEKALKS 
Sbjct: 661 EKRIQWILMEGNDFIGKRKMALAIAELVFGSIEFFLNLNAKSEEMGISRSEIVEKALKST 720

Query: 721 RELVILVEDVDMADSQFMKFLEDGFESGKLGEAEEETIEKVIFVLTKDDSSDKKKNRASS 780
           RELVILVEDV+MADSQFMKFLEDGFESGK GE +EE IEK+IFVLTKDDSSDKKKNR SS
Sbjct: 721 RELVILVEDVEMADSQFMKFLEDGFESGKFGEVKEERIEKLIFVLTKDDSSDKKKNRDSS 780

Query: 781 SSVIEMILKIDARAKPNSDHKRKAEREFENKSKKQRINTYEYENQNNRKEFSRQSIINNI 840
           SSVIEM L+IDAR K    HKRKAERE ENKSKK RIN        NR    + SI NN 
Sbjct: 781 SSVIEMALEIDAREK----HKRKAEREIENKSKKARIN-------KNR----QSSINNNT 840

Query: 841 LDLNLKASNEE----EEEDEETDQKLPNGQISPISSDLTRETTIYDLKPANGFLESISNR 900
           +DLN KA+NEE    E++D ET+Q LPNGQISPISSDLTRETT+YD K ANGFLESISNR
Sbjct: 841 IDLNQKAANEEDQKQEQDDAETNQTLPNGQISPISSDLTRETTMYDRKAANGFLESISNR 900

Query: 901 FILNRKPSQESEITEQLRGKMKGAYKENCKKGNWNWDWDWDWNWDSRFRVEEGVLEGILE 960
           FILN K +QESEI EQLR  M GAY+ENCKK  W+WDWDWDWNWD RFRVEEGVLEGILE
Sbjct: 901 FILNSKSTQESEIREQLRRIMTGAYEENCKKWKWDWDWDWDWNWDCRFRVEEGVLEGILE 960

Query: 961 GFGSFSNKVFEKWVKEIFQTSLEGGRYGGKGEGGIDIRLCLDQKHILEEE----EEEEDG 990
           GFGSFSNKVFEKWVKEIFQTSLEGGRYGGK EGGIDIRLCLDQKHILEEE    EE E+G
Sbjct: 961 GFGSFSNKVFEKWVKEIFQTSLEGGRYGGKQEGGIDIRLCLDQKHILEEEHEEHEEHEEG 994

BLAST of Spg017533 vs. NCBI nr
Match: XP_022965457.1 (protein SMAX1-LIKE 4-like [Cucurbita maxima])

HSP 1 Score: 1381.7 bits (3575), Expect = 0.0e+00
Identity = 772/1034 (74.66%), Postives = 834/1034 (80.66%), Query Frame = 0

Query: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSK---PTLLRRACL 60
           MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLS+    PTLLRRACL
Sbjct: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSNNSKPPTLLRRACL 60

Query: 61  KSHPPHPLHSRALDLCFNVALNRLPTSPPLLHS-PSLSNALIAALKRAQAHQRR------ 120
           KSHPPHPL SRAL+LCFNVALNRLP+SPPLLHS PSLSNALIAALKRAQAHQRR      
Sbjct: 61  KSHPPHPLQSRALELCFNVALNRLPSSPPLLHSPPSLSNALIAALKRAQAHQRRGSSSSS 120

Query: 121 -LDHPPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIEEFN- 180
            LDH          HPLL++KV L HLIISILDDPSVSRVMREAGFSSTAVK NIEE+N 
Sbjct: 121 SLDH---HHQQQQQHPLLTVKVELQHLIISILDDPSVSRVMREAGFSSTAVKTNIEEYND 180

Query: 181 ----------SATTPISS-----------PLFFSSSSPSLPRNHSSSDAAANFVFELFLR 240
                     S  +PISS           P FFSSSSP  P     +  A   VFE FL 
Sbjct: 181 NNNNNNTIFISPPSPISSHFFSAQTNSYTPFFFSSSSPPPP----PTTDATKLVFEAFLG 240

Query: 241 RSN----SNVVVVGDSVGVTEGVVFEVMRKLKTGEVPHQMKGVKFVELLPPFMGISSSSS 300
           ++N    +NVVVVGDSVG+TEGVVFEVMRK+K GEVP  MKGVKFVE LP   G SS   
Sbjct: 241 KNNNNLRTNVVVVGDSVGLTEGVVFEVMRKVKMGEVPEAMKGVKFVEFLPLMKGSSS--- 300

Query: 301 PSPSSSELRRKLRECCCGSDDADNMGVVVYVGDLKWIVEGSN-EEVDGLVGEIERLLIKG 360
                     KL E     D+ D  GV+VYVGDLKWIVEG N +E++ LVGEIERLL KG
Sbjct: 301 ---------LKLGEYV--KDNGDG-GVLVYVGDLKWIVEGGNSDEIERLVGEIERLL-KG 360

Query: 361 DSSFNGDGNTFGSNKVKIWLIAVASYQVYMRCQMRQPALETQWALHALPLPSSGLALTLH 420
           D   N + N    +K KIW++ +ASYQ+YMRCQMRQPALETQW+LHA+P+PSSGL LTLH
Sbjct: 361 D-FLNANNN---GSKAKIWVMGMASYQIYMRCQMRQPALETQWSLHAVPVPSSGLGLTLH 420

Query: 421 ASSVYDSRLSFFSQPMETKPFLAKEEQEKLTCCAECTSNFENEAQHLKSFQSNQVPSWLQ 480
            SSVYDSR SFFSQ METK F+AKEE EKLTCCAECTSNFENE QHLKSFQS QVPSWLQ
Sbjct: 421 TSSVYDSRPSFFSQTMETKQFIAKEEHEKLTCCAECTSNFENEVQHLKSFQSKQVPSWLQ 480

Query: 481 QYNANQSHSKDEYVELRRKWNRFCSSLHRDGSVQSFMAGKSFSYSSSYPWWPKFHESNSI 540
           QYN NQSHSKDE+VELR+KWNRFCSSLHRDGS QS M GKSFSY SSYPWWPK  ESNSI
Sbjct: 481 QYNVNQSHSKDEFVELRKKWNRFCSSLHRDGSAQSLM-GKSFSYCSSYPWWPKIDESNSI 540

Query: 541 SFTDHQTTKPLQSSSFVPRFRRQQSCTTIEFDFGNATTKHDQGRE--PSLNSLKHMVGKE 600
           SFTD+QT KPLQSS+FVP FRRQQSCTTIEFDFGNATTK  Q RE  PSLNSLKHMVGKE
Sbjct: 541 SFTDNQTPKPLQSSNFVPPFRRQQSCTTIEFDFGNATTKQSQDRESSPSLNSLKHMVGKE 600

Query: 601 VKITLALGSPLFCDSSADSMEMESERNSQRGEILKVLQENVPWQSESLPCVAEAVISAKK 660
           VKITLALG+PLF DSSA+SMEMESER ++RGEILKVLQENVPW SESLP +AE VISAKK
Sbjct: 601 VKITLALGNPLFYDSSAESMEMESERKTERGEILKVLQENVPWLSESLPSIAETVISAKK 660

Query: 661 NEKSIEWILIEGNDFIGKRKLALAIAESAFGSVDSLLNLNAKSEEMGISRCEMVEKALKS 720
           NEK I+WIL+EGNDFIGKRK+ALAIAES FGS++  LNLN+KSEEMGISR EMVEKALKS
Sbjct: 661 NEKRIQWILMEGNDFIGKRKMALAIAESVFGSIEFFLNLNSKSEEMGISRSEMVEKALKS 720

Query: 721 NRELVILVEDVDMADSQFMKFLEDGFESGKLGEAEEETIEKVIFVLTKDDSSDKKKNRAS 780
            RELVILVEDV+MADSQFMKFLEDGFESGK GE +EE+IEK+IFVLTKDDSSDKKKNR S
Sbjct: 721 TRELVILVEDVEMADSQFMKFLEDGFESGKFGEVKEESIEKLIFVLTKDDSSDKKKNRDS 780

Query: 781 SSSVIEMILKIDARAKPNSDHKRKAEREFENKSKKQRINTYEYENQNNRKEFSRQSIINN 840
           SSSVIEM L+IDAR K    H RKAERE ENKSKK RIN     + N           NN
Sbjct: 781 SSSVIEMALEIDAREK----HMRKAEREIENKSKKARINKNRQSSTN-----------NN 840

Query: 841 ILDLNLKASNEE----EEEDEETDQKLPNGQISPISSDLTRETTIYDLKPANGFLESISN 900
            +DLN KA+NEE    E++D ET+Q LPNGQISPISSDLTRETT+Y+LKPANGF+ESISN
Sbjct: 841 TIDLNQKAANEEDQKQEQDDAETNQTLPNGQISPISSDLTRETTMYNLKPANGFMESISN 900

Query: 901 RFILNRKPSQESEITEQLRGKMKGAYKENCKKGNWNWDWDWDWNWDSRFRVEEGVLEGIL 960
           RF+L  K +QESEI EQLR  M GAY+ENCKK    W WDWDWNWD RFRVEEGVLEGI+
Sbjct: 901 RFLLKSKSTQESEIREQLRRIMTGAYEENCKK----WKWDWDWNWDCRFRVEEGVLEGIV 960

Query: 961 EGFGSFSNKVFEKWVKEIFQTSLEGGRYGGKGEGGIDIRLCLDQKHILEEE-EEEEDGYM 990
           EGFGSFSNKVFEKWVKEIFQTSLEGGRYGGK EGGIDIRLCLDQKHILEEE EE E+GYM
Sbjct: 961 EGFGSFSNKVFEKWVKEIFQTSLEGGRYGGKQEGGIDIRLCLDQKHILEEEHEEHEEGYM 987

BLAST of Spg017533 vs. NCBI nr
Match: KAG7020936.1 (Protein SMAX1-LIKE 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1365.5 bits (3533), Expect = 0.0e+00
Identity = 772/1036 (74.52%), Postives = 831/1036 (80.21%), Query Frame = 0

Query: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSK---PTLLRRACL 60
           MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLS+    PTLLRRACL
Sbjct: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSNNSKPPTLLRRACL 60

Query: 61  KSHPPHPLHSRALDLCFNVALNRLPTSPPLLHS-PSLSNALIAALKRAQAHQRR------ 120
           KSHPPHPL SRAL+LCFNVALNRLP+SPPLLHS PSLSNALIAALKRAQAHQRR      
Sbjct: 61  KSHPPHPLQSRALELCFNVALNRLPSSPPLLHSPPSLSNALIAALKRAQAHQRRGSSSSS 120

Query: 121 --LDHPPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIEEFN 180
             LDH          HPLL++KV L HLIISILDDPSVSRVMREAGFSSTAVK NIEE+N
Sbjct: 121 SSLDH---HHQQQQQHPLLTVKVELQHLIISILDDPSVSRVMREAGFSSTAVKTNIEEYN 180

Query: 181 ----------SATTPISS-----------PLFFSSSSPSLPRNHSSSDAAANFVFELFLR 240
                     S  +PISS           P FFSSSSP  P     +  A   VFE FL 
Sbjct: 181 DNNNNNTIFISPPSPISSHFFSPQTNTYTPFFFSSSSPPPP---PPTTDATKLVFEAFLG 240

Query: 241 RSN----SNVVVVGDSVGVTEGVVFEVMRKLKTGEVPHQMKGVKFVELLPPFMGISSSSS 300
           ++N    +NVVVVGDSVG+TEGVVFEV+RK+K GEVP  MKGVKFVE LP   G SS   
Sbjct: 241 KNNNNLRTNVVVVGDSVGLTEGVVFEVIRKVKMGEVPEVMKGVKFVEFLPLMKGSSS--- 300

Query: 301 PSPSSSELRRKLRECCCGSDDADNMGVVVYVGDLKWIVEGSN-EEVDGLVGEIERLLIKG 360
                     KL E     D+ D  GV+VYVGDLKWIVEG N +E++ LVGEIER L KG
Sbjct: 301 ---------LKLGEYV--KDNGDG-GVLVYVGDLKWIVEGGNSDEIERLVGEIERSL-KG 360

Query: 361 DSSFNGDGNTFGSNKVKIWLIAVASYQVYMRCQMRQPALETQWALHALPLPSSGLALTLH 420
           D   N + N    +K KIW++ +ASYQ+YMRCQMRQPALETQW+LHA+P+PSSGL LTLH
Sbjct: 361 D-FLNANNN---GSKAKIWVMGMASYQIYMRCQMRQPALETQWSLHAVPVPSSGLGLTLH 420

Query: 421 ASSVYDSRLSFFSQPMETKPFLAKEEQEKLTCCAECTSNFENEAQHLKSFQSNQVPSWLQ 480
            SSVYDSR SFFSQ METK F+AKEE EKLTCCAECTSNFENE QHLKSFQS QVPSWLQ
Sbjct: 421 TSSVYDSRPSFFSQTMETKQFIAKEEHEKLTCCAECTSNFENEVQHLKSFQSKQVPSWLQ 480

Query: 481 QYNANQSHSKDEYVELRRKWNRFCSSLHRDGSVQSFMAGKSFSYSSSYPWWPKFHESNSI 540
           QYN NQSHSKDE+VELR+KWNRFCSSLHRDGS QS M GKSFSY SSYPWWPKF ESNSI
Sbjct: 481 QYNVNQSHSKDEFVELRKKWNRFCSSLHRDGSAQSLM-GKSFSYCSSYPWWPKFDESNSI 540

Query: 541 SFTDHQTTKPLQSSSFVPRFRRQQSCTTIEFDFGNATTKHDQGRE-PSLNSLKHMVGKEV 600
           SFTD+QT KPLQSS+ VPRFRRQQSCTTIEFDFGNATTK  Q RE PSLNSLKHMVGKEV
Sbjct: 541 SFTDNQTPKPLQSSNLVPRFRRQQSCTTIEFDFGNATTKQSQDRESPSLNSLKHMVGKEV 600

Query: 601 KITLALGSPLFCDSSADSMEMESERNSQRGEILKVLQENVPWQSESLPCVAEAVISAKKN 660
           KITLALG+PLF DSSA+SMEMESER ++RGEILKVLQEN  + S     +AE VISAKKN
Sbjct: 601 KITLALGNPLFYDSSAESMEMESERKTERGEILKVLQENNRFHS-----IAETVISAKKN 660

Query: 661 EKSIEWILIEGNDFIGKRKLALAIAESAFGSVDSLLNLNAKSEEMGISRCEMVEKALKSN 720
           EK I+WIL+EGNDFIGKRK+ALAIAE  FGS++  LNLNAKSEEMGISR EMVEKALKS 
Sbjct: 661 EKRIQWILMEGNDFIGKRKMALAIAELVFGSIEFFLNLNAKSEEMGISRSEMVEKALKST 720

Query: 721 RELVILVEDVDMADSQFMKFLEDGFESGKLGEAEEETIEKVIFVLTKDDSSDKKKNRASS 780
           RELVILVEDV+MADSQFMKFLEDGFESGK GE +EE IEK+IFVLTKDDSSDKKKNR SS
Sbjct: 721 RELVILVEDVEMADSQFMKFLEDGFESGKFGEVKEERIEKLIFVLTKDDSSDKKKNRDSS 780

Query: 781 SSVIEMILKIDARAKPNSDHKRKAEREFENKSKKQRINTYEYENQNNRKEFSRQSIINNI 840
           SSVIEM L+IDAR K    HKRKAERE ENKSKK RIN        NR    + SI NN 
Sbjct: 781 SSVIEMALEIDAREK----HKRKAEREIENKSKKARIN-------KNR----QSSINNNT 840

Query: 841 LDLNLKASNEE----EEEDEETDQKLPNGQISPISSDLTRETTIYDLKPANGFLESISNR 900
           +DLN KA+NEE    E++D ET+Q LPNGQISPISSDLTRETT+YD K ANGFLESISNR
Sbjct: 841 IDLNQKAANEEDQKQEQDDAETNQTLPNGQISPISSDLTRETTMYDRKAANGFLESISNR 900

Query: 901 FILNRKPSQESEITEQLRGKMKGAYKENCKKGNWNWDWDWDWNWDSRFRVEEGVLEGILE 960
           FILN K +QESEI EQLR  M GAY+ENCKK  W WDWDWDWNW+ RFRVEEGVLEGILE
Sbjct: 901 FILNSKSTQESEIREQLRRIMTGAYEENCKK--WKWDWDWDWNWNCRFRVEEGVLEGILE 960

Query: 961 GFGSFSNKVFEKWVKEIFQTSLEGGRYGGKGEGGIDIRLCLDQKHILEEE----EEEEDG 990
           GFGSFSNKVFEKWVKEIFQTSLEGGRYGGK EGGIDIRLCLDQKHILEEE    EE E+G
Sbjct: 961 GFGSFSNKVFEKWVKEIFQTSLEGGRYGGKQEGGIDIRLCLDQKHILEEEHEEHEEHEEG 987

BLAST of Spg017533 vs. ExPASy Swiss-Prot
Match: Q9SZR3 (Protein SMAX1-LIKE 4 OS=Arabidopsis thaliana OX=3702 GN=SMXL4 PE=1 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 5.8e-154
Identity = 426/1089 (39.12%), Postives = 583/1089 (53.54%), Query Frame = 0

Query: 1    MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLL-SSKPTLLRRACLKS 60
            MR+G     QTLTPEAASVLK SL+LA RRGHS VTPLHVASTLL SS+  L RRACLKS
Sbjct: 1    MRTGAYTVHQTLTPEAASVLKQSLTLARRRGHSQVTPLHVASTLLTSSRSNLFRRACLKS 60

Query: 61   HP--------PHP-LHSRALDLCFNVALNRLPTSP-PLLHS-PSLSNALIAALKRAQAHQ 120
            +P         HP LH RAL+LCFNV+LNRLPT+P PL  + PSLSNAL+AALKRAQAHQ
Sbjct: 61   NPFTALGRQMAHPSLHCRALELCFNVSLNRLPTNPNPLFQTQPSLSNALVAALKRAQAHQ 120

Query: 121  RRLDHPPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIEEFN 180
            RR         S  + P L++KV L  L++SILDDPSVSRVMREAG SS +VK+NIE+ +
Sbjct: 121  RR--GCVEQQQSQQNQPFLAVKVELEQLVVSILDDPSVSRVMREAGLSSVSVKSNIEDDS 180

Query: 181  SATTPI------SSPLFFSSSSPS----------------------LPRNHS-------- 240
            S  +P+      S  +F S  SPS                      L  +HS        
Sbjct: 181  SVVSPVFYGSSSSVGVFSSPCSPSSSENNQGGGTLSPNPSKIWHAHLTNHHSFEQNPFFH 240

Query: 241  -------SSDAA------ANFVFELFLRRSNS---NVVVVGDSVGVTEGVVFEVMRKLKT 300
                   + D A      AN V E+ L + N+   N V+VGDSV +TEGVV ++M +++ 
Sbjct: 241  FPKGKTFTPDQAFPVREDANPVIEVLLGKKNNKKRNTVIVGDSVSLTEGVVAKLMGRIER 300

Query: 301  GEVPHQMKGVKFVELLPPFMGISSSSSPSPSSS--ELRRKLRECCCGSDDADNMGVVVYV 360
            GEVP  +K   F++     +G++            EL+RK+             GV+V +
Sbjct: 301  GEVPDDLKQTHFIKFQFSQVGLNFMKKEDIEGQVRELKRKIDSFTSWG----GKGVIVCL 360

Query: 361  GDLKWIVEGSNEE--------VDGLVGEIERLLIKGDSSFNGDGNTFGSNKVKIWLIAVA 420
            GDL W V G             D LV EI RL+             + +   K+WL+  A
Sbjct: 361  GDLDWAVWGGGNSASSSNYSAADHLVEEIGRLVY-----------DYSNTGAKVWLLGTA 420

Query: 421  SYQVYMRCQMRQPALETQWALHALPLPSSGLALTLHASSVYDSRLSFFSQPMETKPFLAK 480
            SYQ YMRCQM+QP L+  WAL A+ +PS GL+LTLHASS         SQ ME KPF  K
Sbjct: 421  SYQTYMRCQMKQPPLDVHWALQAVSIPSGGLSLTLHASS-----SEMASQVMEMKPFRVK 480

Query: 481  EEQE---------KLTCCAECTSNFENEAQHLKSFQSNQVPSWLQQYNANQS-HSKDEYV 540
            EE+E         KL  C EC  N+E EA+   S Q   +P WLQ +  N + + KDE  
Sbjct: 481  EEEEGAREEEEEDKLNFCGECAFNYEKEAKAFISAQHKILPPWLQPHGDNNNINQKDELS 540

Query: 541  ELRRKWNRFCSSLHRDGSVQSFMAGKSFSYSSSYPWWPKFHESNSISFTDHQTTKPLQSS 600
             LR+KWNRFC +LH      S  A ++   SS  P           S  D    +  ++S
Sbjct: 541  GLRKKWNRFCQALHH--KKPSMTAWRAEQSSSVLPG----------SLMDSSLKQNSRAS 600

Query: 601  SFVPRFRRQQSCTTIEFDFG-NATTKHDQGREPSLNSLK--HMVGKEVKITLALGSPLFC 660
            S V +FRRQ SC TIEF FG N      +  E SL+  K  +  G + KITLALG   F 
Sbjct: 601  SSVAKFRRQNSC-TIEFSFGSNRQEGLKKTDELSLDGFKSNNDEGVKTKITLALGHSPFP 660

Query: 661  DSSADSMEMESERNSQRGEILKVLQENVPWQSESLPCVAEAV-ISAKKNEKSIEWILIEG 720
              S +S E E E+  +  ++L+ L EN+PWQ + LP + EA+  S K++++   W+L+ G
Sbjct: 661  SDSENSEEEEPEKAIKMSKLLEKLHENIPWQKDVLPSIVEAMEESVKRSKRKDAWMLVSG 720

Query: 721  NDFIGKRKLALAIAESAFGSVDSLLNLNAKSEEMGISRCEMVEKALKSNRELVILVEDVD 780
            ND   KR+LA+ +  S FGS +++L +N ++ +     CE ++ ALK   E+VIL+E VD
Sbjct: 721  NDVTAKRRLAITLTTSLFGSHENMLKINLRTSKAS-EACEELKNALKKKEEVVILIERVD 780

Query: 781  MADSQFMKFLEDGFESGKLGEAEEETIEKVIFVLTKDDSSDKKKNRASSSSVIEMILKID 840
            +AD+QFM  L D FE+G L +  +    ++IF+LT++D    +    +   VI M+L  +
Sbjct: 781  LADAQFMNILVDRFEAGDL-DGFQGKKSQIIFLLTREDDECVE----NEHFVIPMVLNCN 840

Query: 841  ARAKPNSDHKRKAEREFEN---KSKKQRINTYEYEN------QNNRKEFSRQ-SIINNIL 900
                   ++KRK E +      K K  RI   + E+       N +KEFSRQ    +N L
Sbjct: 841  KSGSGLVNNKRKPEYDAAPTMIKKKNPRIEEDDDESNVACDISNIKKEFSRQLKFESNAL 900

Query: 901  DLNLKASNEEEEEDEETDQKLPNGQISPISSDLTRETTIYDLKPANGFLESISNRFILNR 960
            DLNL+   +E+EE+E           + ISS                FL+SI NRF  + 
Sbjct: 901  DLNLRVDADEDEEEEAK-------PATEISSGFEER-----------FLDSIQNRF--DF 960

Query: 961  KPSQESEITEQLRGKMKGAYKENCKKGNWNWDWDWDWNWDSRFRVEEGVLEGILEGFGSF 990
                + +IT+    K+K + +E   +    +           F V+  ++E   +G G F
Sbjct: 961  TVLSDEDITKFFVTKIKDSCEEILGQREERFG----------FTVDAELIEKFYKGCGFF 1017

BLAST of Spg017533 vs. ExPASy Swiss-Prot
Match: Q9LU73 (Protein SMAX1-LIKE 5 OS=Arabidopsis thaliana OX=3702 GN=SMXL5 PE=2 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 4.6e-143
Identity = 398/1102 (36.12%), Postives = 574/1102 (52.09%), Query Frame = 0

Query: 1    MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSKPTLLRRACLKSH 60
            MR+G    QQTLT EAASVLKHSL+LA RRGH+ VTPLHVA+TLLSS+ +LLRRAC+KSH
Sbjct: 1    MRTGGYTIQQTLTTEAASVLKHSLTLARRRGHAQVTPLHVAATLLSSRTSLLRRACIKSH 60

Query: 61   P---------------------PHPLHSRALDLCFNVALNRLPTSP-PLLH-SPSLSNAL 120
            P                      HPL  RAL+LCFNVALNRLPT P P+ H  PSL+NAL
Sbjct: 61   PGFSTNYQFAPSRLQHHHHHNQNHPLQCRALELCFNVALNRLPTVPGPMFHGQPSLANAL 120

Query: 121  IAALKRAQAHQRR----LDHPPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREA 180
            +AALKRAQAHQRR            P      LL++KV L  L+ISILDDPSVSRVMREA
Sbjct: 121  VAALKRAQAHQRRGCIEQQQQTQTHPQTQQTQLLAVKVELEQLVISILDDPSVSRVMREA 180

Query: 181  GFSSTAVKNNIEE---------------FNSAT--------------------------- 240
            GF+STAVK+ +E+               F+S                             
Sbjct: 181  GFNSTAVKSCVEDCSVSSVFYGGSAVGVFSSPNSPDQQQQHHNSINRLHHYQNPKDFNFI 240

Query: 241  --------------TPISSPLFFSSSSPSLPRNHSSSDAAANFVFELFLRR--SNSNVVV 300
                          +P  +PL  SSS+    +     +     V ++ +R+     N V+
Sbjct: 241  NPNFPLWQTHFLNQSPDQNPLLLSSSASHHHQQQRLREIDLKLVVDVLMRKKTKKKNPVI 300

Query: 301  VGDSVGVTEGVVFEVMRKLKTGEVPH--QMKGVKFVE--LLPPFMGISSSSSPSPSSSEL 360
            VGDS+  TEG V E+M KL+ GE+    ++K   FV+    P             +  EL
Sbjct: 301  VGDSISFTEGFVSELMAKLERGEIDQTGELKQTHFVKFHFSPMASKFMRREDVELNIKEL 360

Query: 361  RRKLRECCCGSDDADNMGVVVYVGDLKWIVE--------GSNE------EVDGLVGEIER 420
            R+K+        +A     +++ GDLKW V+        G NE       +D LV EI +
Sbjct: 361  RKKVLSLTTSGKNA-----IIFTGDLKWTVKEITNNNSGGINEISSSYSPLDHLVEEIGK 420

Query: 421  LLIKGDSSFNGDGNTFGSNKVKIWLIAVASYQVYMRCQMRQPALETQWALHALPLPSS-G 480
            L+    +  N DG+       K+W++  AS+Q YMRCQMRQP+LET WALH + +PSS  
Sbjct: 421  LI----TECNDDGDDDDCKTRKVWVMGTASFQTYMRCQMRQPSLETLWALHPVSVPSSAN 480

Query: 481  LALTLHASSVYDSR-LSFFSQPMETKPFLAKEEQEK----LTCCAECTSNFENEAQHLKS 540
            L L+LHA+S +++R +S  +       +   EE+E     L+CC EC ++F+ EA+ LK+
Sbjct: 481  LGLSLHATSGHEARNMSTVNATKSLSGYDKAEEEETISHVLSCCPECVTSFDREAKSLKA 540

Query: 541  FQSNQVPSWLQQYNANQSHSKDEYVELRRKWNRFCSSLHRDGSVQSFMAGKSFSYSSSYP 600
             Q   +PSWLQ ++A+ S  KDE + L+RKWNRFC +LH      S M     +Y    P
Sbjct: 541  NQDKLLPSWLQSHDADSSSQKDELMGLKRKWNRFCETLHNQTGQLSMMG----NYPYGLP 600

Query: 601  WWPKFHESNSISFTDHQTTKPLQ-SSSFVPRFRRQQSCTTIEFDFGNATTKHDQGREPSL 660
            +      S S S  D    KP Q +++ + +FRRQ SC TIEFD G    +H++G   S+
Sbjct: 601  YGSSHESSKSTSLIDSLGLKPNQRATNSIAKFRRQNSC-TIEFDLGG--NEHEKGE--SI 660

Query: 661  NSLKHMVGKEVKITLALGSPLFCDSSADSMEMESERNSQRGEILKVLQENVPWQSESLPC 720
            N  +   G E  +TL LG  LF   S       ++   +   ++K L+E++P Q+ ++  
Sbjct: 661  NEAEDDKGNET-VTLDLGRSLFRSDSV------TDTRLKLSALVKALEESIPRQTVTMRL 720

Query: 721  VAEAVISAKKNEKSIEWILIEGNDFIGKRKLALAIAESAFGSVDSL--LNLNAKSEEMGI 780
            +AE+++     +K   WI+IEG D   KR++A  ++ES FGS +SL  ++L  K  E   
Sbjct: 721  IAESLMDCVSKKKD-SWIIIEGRDTTAKRRVARTVSESVFGSFESLVHIDLKKKGNESKA 780

Query: 781  SRCEMVEKALKSNRELVILVEDVDMADSQFMKFLEDGFESGKLGEAEEETIEKVIFVLTK 840
            S   ++   LK+  ++V L+ED+D+ADS+F+K L D FE  +  +   +   + IF+LTK
Sbjct: 781  SPATLLAYELKNPEKVVFLIEDIDLADSRFLKLLADRFEDKRRIKTGIDH-RQAIFILTK 840

Query: 841  DDSSDKKKNRASSSSVIEMILKIDARAKPNSDHKRKAEREFENKSKKQRINTYEYENQNN 900
            +DS    +N  +  SV+++ L+I A++ P    K +++   EN    +            
Sbjct: 841  EDS----RNVRNRDSVLQIGLEITAQS-PGKKRKPESDLSIENGFWMK------------ 900

Query: 901  RKEFSRQSIIN-NILDLNLKASNEEEEEDEETDQKLPNGQISPISSDLTRETTIYDLKPA 960
            ++  SRQS  N + LDLN+KA +EE E           G+ISPISSDLT E    +   +
Sbjct: 901  KEVCSRQSSFNSSYLDLNIKAEDEEVE-----------GEISPISSDLTGEEET-EFSSS 960

Query: 961  NGFLESISNRFILNRKPSQESEITEQLRGKMKGAYKENCKKGNWNWDWDWDWNWDSRFRV 990
            + FL  I NRF+LNR      E     +G +  A++E           + +     RF V
Sbjct: 961  SNFLNRIQNRFVLNRSCEPGIE-----KGMITAAFREIFP--------EREEGGGVRFSV 1020

BLAST of Spg017533 vs. ExPASy Swiss-Prot
Match: Q9SVD0 (Protein SMAX1-LIKE 3 OS=Arabidopsis thaliana OX=3702 GN=SMXL3 PE=2 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 8.0e-87
Identity = 285/849 (33.57%), Postives = 412/849 (48.53%), Query Frame = 0

Query: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSKPTLLRRACLKSH 60
           MR+G C  +Q LT +AA+V+K ++ LA RRGH+ VTPLHVAST+LS+   LLR ACL+SH
Sbjct: 1   MRAGGCTVEQALTADAANVVKQAMGLARRRGHAQVTPLHVASTMLSAPTGLLRTACLQSH 60

Query: 61  PPHPLHSRALDLCFNVALNRLPTSP-------PLLHSPSLSNALIAALKRAQAHQRRLDH 120
             HPL  RAL+LCFNVALNRLPTS        P    PS+SNAL AA KRAQAHQRR   
Sbjct: 61  -THPLQCRALELCFNVALNRLPTSTGSPMLGVPTSPFPSISNALGAAFKRAQAHQRR--- 120

Query: 121 PPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIE-----EFN 180
                      P+L++K+ +  LIISILDDPSVSRVMREAGFSS  VK  +E     E  
Sbjct: 121 ---GSIESQQQPILAVKIEVEQLIISILDDPSVSRVMREAGFSSPQVKTKVEQAVSLEIC 180

Query: 181 SATTPISSPLFFSSSSPSLPRNHSSSDAAANFVFELFLRRSNSNVVVVGDSVGVTEGVVF 240
           S TT  S P      +P   RN    +   N V      +   N V+VG+ +   +GVV 
Sbjct: 181 SKTTSSSKPKEGKLLTP--VRNEDVMNVINNLV-----DKKRRNFVIVGECLATIDGVVK 240

Query: 241 EVMRKLKTGEVPHQMKGVKFVELLPPFMGISSSSSPSPSSSELRRKLRECCCGSDDADNM 300
            VM K+   +VP  +K VKF+ L       S SS   PS +++ RKL E           
Sbjct: 241 TVMEKVDKKDVPEVLKDVKFITL-------SFSSFGQPSRADVERKLEELETLVKSCVGK 300

Query: 301 GVVVYVGDLKWIVE---------GSNEE---VDGLVGEIERL---LIKGDSSFNGDGNTF 360
           GV++ +GDL W VE          +N+    V+ ++ EI +L   L+ GD          
Sbjct: 301 GVILNLGDLNWFVESRTRGSSLYNNNDSYCVVEHMIMEIGKLACGLVMGDHG-------- 360

Query: 361 GSNKVKIWLIAVASYQVYMRCQMRQPALETQWALHALPLPSSGLALTLHASSVYDSRLSF 420
                + WL+ +A+ Q Y+RC+  QP+LE+ W L  L +P++  +L L  S V +S L  
Sbjct: 361 -----RFWLMGLATSQTYVRCKSGQPSLESLWCLTTLTIPATSNSLRL--SLVSESELEV 420

Query: 421 FSQPMETKPFLAKEEQEKLTCCAECTSNFENEAQHLKSFQSN----QVPSWLQQY---NA 480
             +  E      ++  ++L+ C EC+  FE+EA+ LKS  SN     +P+WLQQY   N 
Sbjct: 421 --KKSENVSLQLQQSSDQLSFCEECSVKFESEARFLKSSNSNVTTVALPAWLQQYKKENQ 480

Query: 481 NQSHSKDEYVELRRKWNRFCSSLHRDGSVQSF-MAGKSFSYSSSYPWWPKFHESNSISFT 540
           N     D   EL  KWN  C S+H+  S+++  ++  + S+S S           SIS  
Sbjct: 481 NSHTDSDSIKELVVKWNSICDSIHKRPSLKTLTLSSPTSSFSGS--------TQPSISTL 540

Query: 541 DHQTTKPLQSSSFVPRFRRQQSCTTIEFDFGNATTKHDQGREPS-LNSLKHMVGKEVKIT 600
            H     LQ++   P          IE    N    H    E S L         E K  
Sbjct: 541 HH-----LQTNGDWP---------VIE---TNTHRHHSVVHETSHLRLFIPEHDSEQKTE 600

Query: 601 LALGSPLFC----DSSADSMEME------SERNSQR-GEILKVLQENVPWQSESLPCVAE 660
           L   +P        SS+D+ME+E       E N++    +   L+  VPWQ + +P +A+
Sbjct: 601 LVCSNPNSTMNSEASSSDAMELEHASSRFKEMNAENLATLCAALESKVPWQKDLVPELAK 660

Query: 661 AV-----------ISAKKNEKSIEWILIEGNDFIGKRKLALAIAESAFGSVDSLLNLNAK 720
            V           I+  +++K   W+  +G D   K K+A  +A+  FGS DS +++   
Sbjct: 661 TVLKCRSGSSTRKINGNEDKKEDTWMFFQGLDVDAKEKIARELAKLVFGSQDSFVSICLS 720

Query: 721 S------------------EEMGISRCEMVEKALKSNRELVILVEDVDMADSQFMKFLED 754
           S                  +E  +S  E   +A+  +   VILVED++ AD       + 
Sbjct: 721 SFSSTRSDSAEDLRNKRLRDEQSLSYIERFSEAVSLDPNRVILVEDIEQADYLSQVGFKR 780

BLAST of Spg017533 vs. ExPASy Swiss-Prot
Match: Q9FHH2 (Protein SUPPRESSOR OF MAX2 1 OS=Arabidopsis thaliana OX=3702 GN=SMAX1 PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 1.6e-71
Identity = 253/806 (31.39%), Postives = 379/806 (47.02%), Query Frame = 0

Query: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSKPTLLRRACLKSH 60
           MR+G    QQTLTPEAA+VL  S++ AARR H   TPLHVA+TLL+S    LRRAC++SH
Sbjct: 1   MRAGLSTIQQTLTPEAATVLNQSIAEAARRNHGQTTPLHVAATLLASPAGFLRRACIRSH 60

Query: 61  P--PHPLHSRALDLCFNVALNRLPTSPPLL-HSPSLSNALIAALKRAQAHQRRLDHPPPP 120
           P   HPL  RAL+LCF+VAL RLPT+     + P +SNAL+AALKRAQAHQRR       
Sbjct: 61  PNSSHPLQCRALELCFSVALERLPTATTTPGNDPPISNALMAALKRAQAHQRR------G 120

Query: 121 PPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIEE-FNSATTPISS 180
            P     PLL++KV L  LIISILDDPSVSRVMREA FSS AVK  IE+  N++ TP   
Sbjct: 121 CPEQQQQPLLAVKVELEQLIISILDDPSVSRVMREASFSSPAVKATIEQSLNNSVTPTPI 180

Query: 181 PLFFSSS-----------------SPSLPRNHSSSDAAAN------FVFELFLRRSNSNV 240
           P   S                   +P L +N SS  +  +       V ++  R    N 
Sbjct: 181 PSVSSVGLNFRPGGGGPMTRNSYLNPRLQQNASSVQSGVSKNDDVERVMDILGRAKKKNP 240

Query: 241 VVVGDSVGVTEGVVFEVMRKLKTGEVPH-QMKGVKFVELLPPFMGISSSSSPSPSSSELR 300
           V+VGDS      V+ E+++K++ GEV +  +K  K V L         SS  +    EL 
Sbjct: 241 VLVGDS--EPGRVIREILKKIEVGEVGNLAVKNSKVVSL------EEISSDKALRIKELD 300

Query: 301 RKLRECCCGSDDADNMGVVVYVGDLKWIVEGSN----------EEVDGLVGEIERLLIKG 360
             L+     SD     GV++ +GDLKW+VE  +          E     V E+ RLL K 
Sbjct: 301 GLLQTRLKNSDPIGGGGVILDLGDLKWLVEQPSSTQPPATVAVEIGRTAVVELRRLLEK- 360

Query: 361 DSSFNGDGNTFGSNKVKIWLIAVASYQVYMRCQMRQPALETQWALHALPLPSSGLALTLH 420
              F G          ++W I  A+ + Y+RCQ+  P++ET W L A+      +A    
Sbjct: 361 ---FEG----------RLWFIGTATCETYLRCQVYHPSVETDWDLQAV-----SVAAKAP 420

Query: 421 ASSVYDSRLSFFSQPMETKPFLAKEEQEKLTCCAECTSNFENEAQHLKSF---------- 480
           AS V+    +        K F+       L CC +C  ++E E   + S           
Sbjct: 421 ASGVFPRLANNLESFTPLKSFV--PANRTLKCCPQCLQSYERELAEIDSVSSPEVKSEVA 480

Query: 481 QSNQVPSWLQQYNANQSHSKDEYVELRRKWNRFCSSLH--------RDGSVQSFMAGKSF 540
           Q  Q+P WL +        + +  E+++KWN  C  LH        R   +   +   + 
Sbjct: 481 QPKQLPQWLLKAKPVDRLPQAKIEEVQKKWNDACVRLHPSFHNKNERIVPIPVPITLTTS 540

Query: 541 SYSSS----YPWWPKFHESNSISFTDHQTTKPLQSSSFVPRFRRQQSCTTIEFD--FGNA 600
            YS +     P  PK   +  +    H   KP+         ++    + ++ D   G A
Sbjct: 541 PYSPNMLLRQPLQPKLQPNRELRERVH--LKPMSPLVAEQAKKKSPPGSPVQTDLVLGRA 600

Query: 601 TTKHDQGREPSLNSLKHMVGKEVKITLALGSPLFCDSSADSMEMESERNSQRGEILKVLQ 660
                 G     + L  +  + V+    + S L  ++  +S++++  +     ++LK + 
Sbjct: 601 EDSEKAGDVQVRDFLGCISSESVQNNNNI-SVLQKENLGNSLDIDLFK-----KLLKGMT 660

Query: 661 ENVPWQSESLPCVAEAVISAKKN--------EKSIEWILIEGNDFIGKRKLALAIAESAF 720
           E V WQ+++   VA  V   K           K   W+L  G D +GKRK+  A++   +
Sbjct: 661 EKVWWQNDAAAAVAATVSQCKLGNGKRRGVLSKGDVWLLFSGPDRVGKRKMVSALSSLVY 720

Query: 721 GSVDSLLNLNAK-------SEEMGISRCEMVEKALKSNRELVILVEDVDMADSQFMKFLE 728
           G+   ++ L ++       S   G +  + + + +K +   VIL+ED+D AD      ++
Sbjct: 721 GTNPIMIQLGSRQDAGDGNSSFRGKTALDKIAETVKRSPFSVILLEDIDEADMLVRGSIK 763

BLAST of Spg017533 vs. ExPASy Swiss-Prot
Match: Q9M0C5 (Protein SMAX1-LIKE 2 OS=Arabidopsis thaliana OX=3702 GN=SMXL2 PE=2 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 2.4e-62
Identity = 279/1005 (27.76%), Postives = 431/1005 (42.89%), Query Frame = 0

Query: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSKPTLLRRACLKSH 60
           MR+     QQTLTPEAA+VL  S++ A RR H H TPLHVA+TLLSS    LR+AC+KSH
Sbjct: 1   MRADLITIQQTLTPEAATVLNQSIAEATRRNHGHTTPLHVAATLLSSSSGYLRQACIKSH 60

Query: 61  P--PHPLHSRALDLCFNVALNRLPT--------------SPPLLHSPSLSNALIAALKRA 120
           P   HPL  RAL+LCF+VAL RLPT              SP     P LSNAL AALKRA
Sbjct: 61  PNSSHPLQCRALELCFSVALERLPTTSTTTTTTSSSSSSSPSQTQEPLLSNALTAALKRA 120

Query: 121 QAHQRRLDHPPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNI 180
           QAHQRR        P     PLL++KV L  LIISILDDPSVSRVMREA FSS AVK+ I
Sbjct: 121 QAHQRR------GCPEQQQQPLLAVKVELEQLIISILDDPSVSRVMREASFSSPAVKSAI 180

Query: 181 EE---------------------------FNSATTPISSPLFFSS--SSPSLPRNHS--- 240
           E+                           + S   P++  L+ +     P +        
Sbjct: 181 EQSLIGNSVSNSRQTGSPGIINPSAIGFGYRSVPAPVNRNLYLNPRLQQPGVGMQSGMMI 240

Query: 241 SSDAAANFVFELFLRRSNSNVVVVGDSVGVTEGVVFEVMRKLKTGEVPH-QMKGVKFVEL 300
                A  V E+ +R    N V+VGDS      +V E++ K++ GE     ++  + + L
Sbjct: 241 QRTDEAKRVIEIMIRTRKRNPVLVGDS--EPHILVKEILEKIENGEFSDGALRNFQVIRL 300

Query: 301 LPPFMGISSSSSPSPSSSELRRKLRECC-CGSDDADNMGVVVYVGDLKWIVEGSNEEVDG 360
               +            S+L  +L E            GVV+ +GDLKW+VE       G
Sbjct: 301 EKELV------------SQLATRLGEISGLVETRIGGGGVVLDLGDLKWLVEHPAAN-GG 360

Query: 361 LVGEIERLLIKGDSSFNGDGNTFGSNKVKIWLIAVASYQVYMRCQMRQPALETQWALHAL 420
            V E+ +LL +               K ++  I  A+ + Y+RCQ+  P++E  W L A+
Sbjct: 361 AVVEMRKLLER--------------YKGRLCFIGTATCETYLRCQVYYPSMENDWDLQAI 420

Query: 421 PL--PSSGLALTLHASSVYDSRLSFFSQPM-------ETKPFLAKEEQEKLTCCAECTSN 480
           P+   SS  A+     S  ++     S  +        T+ F  +    K++CC+ C  +
Sbjct: 421 PIAAKSSLPAIFPRLGSNNNNNAMLLSNNIISIESISPTRSF--QIPMSKMSCCSRCLQS 480

Query: 481 FENEA----QHLKSFQSNQVPSWLQQYNANQSHSK-----DEYVELRRKWNRFCSSLHRD 540
           +EN+     + L     + +P WLQ   AN    K      + VEL++KWN  C  LH +
Sbjct: 481 YENDVAKVEKDLTGDNRSVLPQWLQNAKANDDGDKKLTKDQQIVELQKKWNDLCLRLHPN 540

Query: 541 GSVQSFMAGKSFSYSSSYPWWPKFHESNSISFTDHQTTKPLQSSSFVPRFRRQQSCTTIE 600
            SV   +A  + S         K +  + I+        P+ +   + R  R  S     
Sbjct: 541 QSVSERIAPSTLSMM-------KINTRSDIT----PPGSPVGTDLVLGRPNRGLS----- 600

Query: 601 FDFGNATTKHDQGREPSLNSLKHMVGKEVKITLALGSPLFCDSSADSMEMESERNSQRGE 660
                          P   + +   GK       LG         DS +++  +     +
Sbjct: 601 --------------SPEKKTREARFGK-------LG---------DSFDIDLFK-----K 660

Query: 661 ILKVLQENVPWQSESLPCVAEAVISAKK---NEKSIEWILIEGNDFIGKRKLALAIAESA 720
           +LK L ++V WQ ++   VA A+   K      K   W++  G D  GK K+A A+++  
Sbjct: 661 LLKGLAKSVWWQHDAASSVAAAITECKHGNGKSKGDIWLMFTGPDRAGKSKMASALSDLV 720

Query: 721 FGSVDSLLNLNAKSEE------MGISRCEMVEKALKSNRELVILVEDVDMADSQFMKFLE 780
            GS    ++L + S         G +  +   +A++ N   VI++ED+D AD      ++
Sbjct: 721 SGSQPITISLGSSSRMDDGLNIRGKTALDRFAEAVRRNPFAVIVLEDIDEADILLRNNVK 780

Query: 781 DGFESGKLGEA--EEETIEKVIFVLTKDDSSDKKKNRASSSSVIEMILKIDARAKPNSDH 840
              E G++ ++   E ++  VI +LT + S    KN AS       I +    +  N   
Sbjct: 781 IAIERGRICDSYGREVSLGNVIIILTANSSLGSAKNVAS-------IDETRLESLVNKGW 840

Query: 841 KRKAEREFENKSKKQRINTYEYENQNNRKEFSRQSIINNILDLNLKASNEEEEEDEETDQ 900
           + +      +K++K++ N    +N   ++       +N   + +  +S+   E D+E + 
Sbjct: 841 ELRLSVCNSSKTRKRKPNWLYSDNDQTKQRKEICFDLNEAAEFD-SSSDVTVEHDQEDNG 890

Query: 901 KLPNGQISPISSDLTRETTIYDLKPANGFLESISNRFILNRKPSQESEITEQLRGKMKGA 927
            L +  +  +   +      +D    +   ES+  RF          EI +    ++ GA
Sbjct: 901 NLVHKLVGLVDDAILFRPVDFD-SIKSKTAESLKKRFSNGLADGLTVEIEDDALERIAGA 890

BLAST of Spg017533 vs. ExPASy TrEMBL
Match: A0A6J1FHA9 (protein SMAX1-LIKE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111444147 PE=4 SV=1)

HSP 1 Score: 1392.5 bits (3603), Expect = 0.0e+00
Identity = 779/1036 (75.19%), Postives = 838/1036 (80.89%), Query Frame = 0

Query: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSK---PTLLRRACL 60
           MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLS+    PTLLRRACL
Sbjct: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSNNSKPPTLLRRACL 60

Query: 61  KSHPPHPLHSRALDLCFNVALNRLPTSPPLLHS-PSLSNALIAALKRAQAHQRR------ 120
           KSHPPHPL SRAL+LCFNVALNRLP+SPPLLHS PSLSNALIAALKRAQAHQRR      
Sbjct: 61  KSHPPHPLQSRALELCFNVALNRLPSSPPLLHSPPSLSNALIAALKRAQAHQRRGSSSSS 120

Query: 121 --LDHPPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIEEFN 180
             LDH          HPLL++KV L HLIISILDDPSVSRVMREAGFSSTAVK NIEE+N
Sbjct: 121 SSLDH---HHQQQQQHPLLTVKVELQHLIISILDDPSVSRVMREAGFSSTAVKTNIEEYN 180

Query: 181 ----------SATTPISS-----------PLFFSSSSPSLPRNHSSSDAAANFVFELFLR 240
                     S  +PISS           P FFSSSSP  P     +  A   VFE FL 
Sbjct: 181 DNNNNNTIFISPPSPISSHFFSPQTNTYTPFFFSSSSPPPP---PPTTDATKLVFEAFLG 240

Query: 241 RSN----SNVVVVGDSVGVTEGVVFEVMRKLKTGEVPHQMKGVKFVELLPPFMGISSSSS 300
           ++N    +NVVVVGDSVG+TEGVVFEV+RK+K GEVP  MKGVKFVE LP   G SS   
Sbjct: 241 KNNNNLRTNVVVVGDSVGLTEGVVFEVIRKVKMGEVPEVMKGVKFVEFLPLMKGSSS--- 300

Query: 301 PSPSSSELRRKLRECCCGSDDADNMGVVVYVGDLKWIVEGSN-EEVDGLVGEIERLLIKG 360
                     KL E     D+ D  GV+VYVGDLKWIVEG N +E++ LVGEIER L KG
Sbjct: 301 ---------LKLGEYV--KDNGDG-GVLVYVGDLKWIVEGGNSDEIERLVGEIERSL-KG 360

Query: 361 DSSFNGDGNTFGSNKVKIWLIAVASYQVYMRCQMRQPALETQWALHALPLPSSGLALTLH 420
           D   N + N    +K KIW++ +ASYQ+YMRCQMRQPALETQW+LHA+P+PSSGL LTLH
Sbjct: 361 D-FLNANNN---GSKAKIWVMGMASYQIYMRCQMRQPALETQWSLHAVPVPSSGLGLTLH 420

Query: 421 ASSVYDSRLSFFSQPMETKPFLAKEEQEKLTCCAECTSNFENEAQHLKSFQSNQVPSWLQ 480
            SSVYDSR SFFSQ METK F+AKEE EKLTCCAECTSNFENE QHLKSFQS QVPSWLQ
Sbjct: 421 TSSVYDSRPSFFSQTMETKQFIAKEEHEKLTCCAECTSNFENEVQHLKSFQSKQVPSWLQ 480

Query: 481 QYNANQSHSKDEYVELRRKWNRFCSSLHRDGSVQSFMAGKSFSYSSSYPWWPKFHESNSI 540
           QYN NQSHSKDE+VELR+KWNRFCSSLHRDGS QS M GKSFSY SSYPWWPKF ESNSI
Sbjct: 481 QYNVNQSHSKDEFVELRKKWNRFCSSLHRDGSAQSLM-GKSFSYCSSYPWWPKFDESNSI 540

Query: 541 SFTDHQTTKPLQSSSFVPRFRRQQSCTTIEFDFGNATTKHDQGRE-PSLNSLKHMVGKEV 600
           SFTD+QT KPLQSS+ VPRFRRQQSCTTIEFDFGNATTK  Q RE PSLNSLKHMVGKEV
Sbjct: 541 SFTDNQTPKPLQSSNLVPRFRRQQSCTTIEFDFGNATTKQSQDRESPSLNSLKHMVGKEV 600

Query: 601 KITLALGSPLFCDSSADSMEMESERNSQRGEILKVLQENVPWQSESLPCVAEAVISAKKN 660
           KITLALG+PLF DSSA+SMEMESER ++RGEILKVLQENVPWQSESL  +AE VISAKKN
Sbjct: 601 KITLALGNPLFYDSSAESMEMESERKTERGEILKVLQENVPWQSESLHSIAETVISAKKN 660

Query: 661 EKSIEWILIEGNDFIGKRKLALAIAESAFGSVDSLLNLNAKSEEMGISRCEMVEKALKSN 720
           EK I+WIL+EGNDFIGKRK+ALAIAE  FGS++  LNLNAKSEEMGISR E+VEKALKS 
Sbjct: 661 EKRIQWILMEGNDFIGKRKMALAIAELVFGSIEFFLNLNAKSEEMGISRSEIVEKALKST 720

Query: 721 RELVILVEDVDMADSQFMKFLEDGFESGKLGEAEEETIEKVIFVLTKDDSSDKKKNRASS 780
           RELVILVEDV+MADSQFMKFLEDGFESGK GE +EE IEK+IFVLTKDDSSDKKKNR SS
Sbjct: 721 RELVILVEDVEMADSQFMKFLEDGFESGKFGEVKEERIEKLIFVLTKDDSSDKKKNRDSS 780

Query: 781 SSVIEMILKIDARAKPNSDHKRKAEREFENKSKKQRINTYEYENQNNRKEFSRQSIINNI 840
           SSVIEM L+IDAR K    HKRKAERE ENKSKK RIN        NR    + SI NN 
Sbjct: 781 SSVIEMALEIDAREK----HKRKAEREIENKSKKARIN-------KNR----QSSINNNT 840

Query: 841 LDLNLKASNEE----EEEDEETDQKLPNGQISPISSDLTRETTIYDLKPANGFLESISNR 900
           +DLN KA+NEE    E++D ET+Q LPNGQISPISSDLTRETT+YD K ANGFLESISNR
Sbjct: 841 IDLNQKAANEEDQKQEQDDAETNQTLPNGQISPISSDLTRETTMYDRKAANGFLESISNR 900

Query: 901 FILNRKPSQESEITEQLRGKMKGAYKENCKKGNWNWDWDWDWNWDSRFRVEEGVLEGILE 960
           FILN K +QESEI EQLR  M GAY+ENCKK  W+WDWDWDWNWD RFRVEEGVLEGILE
Sbjct: 901 FILNSKSTQESEIREQLRRIMTGAYEENCKKWKWDWDWDWDWNWDCRFRVEEGVLEGILE 960

Query: 961 GFGSFSNKVFEKWVKEIFQTSLEGGRYGGKGEGGIDIRLCLDQKHILEEE----EEEEDG 990
           GFGSFSNKVFEKWVKEIFQTSLEGGRYGGK EGGIDIRLCLDQKHILEEE    EE E+G
Sbjct: 961 GFGSFSNKVFEKWVKEIFQTSLEGGRYGGKQEGGIDIRLCLDQKHILEEEHEEHEEHEEG 994

BLAST of Spg017533 vs. ExPASy TrEMBL
Match: A0A6J1HKD7 (protein SMAX1-LIKE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111465358 PE=4 SV=1)

HSP 1 Score: 1381.7 bits (3575), Expect = 0.0e+00
Identity = 772/1034 (74.66%), Postives = 834/1034 (80.66%), Query Frame = 0

Query: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSK---PTLLRRACL 60
           MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLS+    PTLLRRACL
Sbjct: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSNNSKPPTLLRRACL 60

Query: 61  KSHPPHPLHSRALDLCFNVALNRLPTSPPLLHS-PSLSNALIAALKRAQAHQRR------ 120
           KSHPPHPL SRAL+LCFNVALNRLP+SPPLLHS PSLSNALIAALKRAQAHQRR      
Sbjct: 61  KSHPPHPLQSRALELCFNVALNRLPSSPPLLHSPPSLSNALIAALKRAQAHQRRGSSSSS 120

Query: 121 -LDHPPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIEEFN- 180
            LDH          HPLL++KV L HLIISILDDPSVSRVMREAGFSSTAVK NIEE+N 
Sbjct: 121 SLDH---HHQQQQQHPLLTVKVELQHLIISILDDPSVSRVMREAGFSSTAVKTNIEEYND 180

Query: 181 ----------SATTPISS-----------PLFFSSSSPSLPRNHSSSDAAANFVFELFLR 240
                     S  +PISS           P FFSSSSP  P     +  A   VFE FL 
Sbjct: 181 NNNNNNTIFISPPSPISSHFFSAQTNSYTPFFFSSSSPPPP----PTTDATKLVFEAFLG 240

Query: 241 RSN----SNVVVVGDSVGVTEGVVFEVMRKLKTGEVPHQMKGVKFVELLPPFMGISSSSS 300
           ++N    +NVVVVGDSVG+TEGVVFEVMRK+K GEVP  MKGVKFVE LP   G SS   
Sbjct: 241 KNNNNLRTNVVVVGDSVGLTEGVVFEVMRKVKMGEVPEAMKGVKFVEFLPLMKGSSS--- 300

Query: 301 PSPSSSELRRKLRECCCGSDDADNMGVVVYVGDLKWIVEGSN-EEVDGLVGEIERLLIKG 360
                     KL E     D+ D  GV+VYVGDLKWIVEG N +E++ LVGEIERLL KG
Sbjct: 301 ---------LKLGEYV--KDNGDG-GVLVYVGDLKWIVEGGNSDEIERLVGEIERLL-KG 360

Query: 361 DSSFNGDGNTFGSNKVKIWLIAVASYQVYMRCQMRQPALETQWALHALPLPSSGLALTLH 420
           D   N + N    +K KIW++ +ASYQ+YMRCQMRQPALETQW+LHA+P+PSSGL LTLH
Sbjct: 361 D-FLNANNN---GSKAKIWVMGMASYQIYMRCQMRQPALETQWSLHAVPVPSSGLGLTLH 420

Query: 421 ASSVYDSRLSFFSQPMETKPFLAKEEQEKLTCCAECTSNFENEAQHLKSFQSNQVPSWLQ 480
            SSVYDSR SFFSQ METK F+AKEE EKLTCCAECTSNFENE QHLKSFQS QVPSWLQ
Sbjct: 421 TSSVYDSRPSFFSQTMETKQFIAKEEHEKLTCCAECTSNFENEVQHLKSFQSKQVPSWLQ 480

Query: 481 QYNANQSHSKDEYVELRRKWNRFCSSLHRDGSVQSFMAGKSFSYSSSYPWWPKFHESNSI 540
           QYN NQSHSKDE+VELR+KWNRFCSSLHRDGS QS M GKSFSY SSYPWWPK  ESNSI
Sbjct: 481 QYNVNQSHSKDEFVELRKKWNRFCSSLHRDGSAQSLM-GKSFSYCSSYPWWPKIDESNSI 540

Query: 541 SFTDHQTTKPLQSSSFVPRFRRQQSCTTIEFDFGNATTKHDQGRE--PSLNSLKHMVGKE 600
           SFTD+QT KPLQSS+FVP FRRQQSCTTIEFDFGNATTK  Q RE  PSLNSLKHMVGKE
Sbjct: 541 SFTDNQTPKPLQSSNFVPPFRRQQSCTTIEFDFGNATTKQSQDRESSPSLNSLKHMVGKE 600

Query: 601 VKITLALGSPLFCDSSADSMEMESERNSQRGEILKVLQENVPWQSESLPCVAEAVISAKK 660
           VKITLALG+PLF DSSA+SMEMESER ++RGEILKVLQENVPW SESLP +AE VISAKK
Sbjct: 601 VKITLALGNPLFYDSSAESMEMESERKTERGEILKVLQENVPWLSESLPSIAETVISAKK 660

Query: 661 NEKSIEWILIEGNDFIGKRKLALAIAESAFGSVDSLLNLNAKSEEMGISRCEMVEKALKS 720
           NEK I+WIL+EGNDFIGKRK+ALAIAES FGS++  LNLN+KSEEMGISR EMVEKALKS
Sbjct: 661 NEKRIQWILMEGNDFIGKRKMALAIAESVFGSIEFFLNLNSKSEEMGISRSEMVEKALKS 720

Query: 721 NRELVILVEDVDMADSQFMKFLEDGFESGKLGEAEEETIEKVIFVLTKDDSSDKKKNRAS 780
            RELVILVEDV+MADSQFMKFLEDGFESGK GE +EE+IEK+IFVLTKDDSSDKKKNR S
Sbjct: 721 TRELVILVEDVEMADSQFMKFLEDGFESGKFGEVKEESIEKLIFVLTKDDSSDKKKNRDS 780

Query: 781 SSSVIEMILKIDARAKPNSDHKRKAEREFENKSKKQRINTYEYENQNNRKEFSRQSIINN 840
           SSSVIEM L+IDAR K    H RKAERE ENKSKK RIN     + N           NN
Sbjct: 781 SSSVIEMALEIDAREK----HMRKAEREIENKSKKARINKNRQSSTN-----------NN 840

Query: 841 ILDLNLKASNEE----EEEDEETDQKLPNGQISPISSDLTRETTIYDLKPANGFLESISN 900
            +DLN KA+NEE    E++D ET+Q LPNGQISPISSDLTRETT+Y+LKPANGF+ESISN
Sbjct: 841 TIDLNQKAANEEDQKQEQDDAETNQTLPNGQISPISSDLTRETTMYNLKPANGFMESISN 900

Query: 901 RFILNRKPSQESEITEQLRGKMKGAYKENCKKGNWNWDWDWDWNWDSRFRVEEGVLEGIL 960
           RF+L  K +QESEI EQLR  M GAY+ENCKK    W WDWDWNWD RFRVEEGVLEGI+
Sbjct: 901 RFLLKSKSTQESEIREQLRRIMTGAYEENCKK----WKWDWDWNWDCRFRVEEGVLEGIV 960

Query: 961 EGFGSFSNKVFEKWVKEIFQTSLEGGRYGGKGEGGIDIRLCLDQKHILEEE-EEEEDGYM 990
           EGFGSFSNKVFEKWVKEIFQTSLEGGRYGGK EGGIDIRLCLDQKHILEEE EE E+GYM
Sbjct: 961 EGFGSFSNKVFEKWVKEIFQTSLEGGRYGGKQEGGIDIRLCLDQKHILEEEHEEHEEGYM 987

BLAST of Spg017533 vs. ExPASy TrEMBL
Match: A0A6J1K933 (protein SMAX1-LIKE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111491771 PE=4 SV=1)

HSP 1 Score: 1319.3 bits (3413), Expect = 0.0e+00
Identity = 738/1019 (72.42%), Postives = 820/1019 (80.47%), Query Frame = 0

Query: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSKP---TLLRRACL 60
           MRSG CA+QQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSS P   TL RRACL
Sbjct: 1   MRSGACASQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSNPSTLTLFRRACL 60

Query: 61  KSHP--PHPLHSRALDLCFNVALNRLPTSPPLLHSPSLSNALIAALKRAQAHQRR--LDH 120
           KSHP  PHPL SRAL+LCFNVALNRLPTSPPLLHSPSLSNALIAALKRAQAHQRR  LDH
Sbjct: 61  KSHPPHPHPLQSRALELCFNVALNRLPTSPPLLHSPSLSNALIAALKRAQAHQRRGSLDH 120

Query: 121 PPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIEEFNSATTP 180
               P     HPLL++KV L HL+ISILDDPSVSRVMREAGFSSTAVKNNIE++ + T P
Sbjct: 121 QHQQPTH--QHPLLAIKVELQHLVISILDDPSVSRVMREAGFSSTAVKNNIEDYTNHTNP 180

Query: 181 I--------SSPLFFSSSSPSLPRNHSSSDAAANFVFELFL-RRSNSNVVVVGDSVGVTE 240
                    +SPLFF  S+PS     S++D  + FVFE FL  R   NVVVVGDS+G TE
Sbjct: 181 TNPTFFSPQTSPLFFFPSAPS--ACISNTDNVSKFVFEAFLGMRKRRNVVVVGDSIGGTE 240

Query: 241 GVVFEVMRKLKTGEVPHQMKGVKFVELLPPFMGISSSSSPSPSSS---ELRRKLRECCCG 300
           GVV  VMRK KTGEVP +MKGVKFVE L PFMG+  + + +  S+    LRRKL E    
Sbjct: 241 GVVLGVMRKFKTGEVPEEMKGVKFVEFL-PFMGMLMAGNHNNKSNLCEVLRRKLGE---D 300

Query: 301 SDDADNMGVVVYVGDLKWIVEGSNEEVDGLVGEIERLLIKGDSSFNGDGNTFGSNKVKIW 360
           S +    G VVYVGDLKW+VE  ++EVD LVGEIERLL++G  + N        +KVKIW
Sbjct: 301 SGNGGGGGAVVYVGDLKWVVERDSKEVDELVGEIERLLLRGFDNHN--------HKVKIW 360

Query: 361 LIAVASYQVYMRCQMRQPALETQWALHALPLP-SSGLALTLHASSVYDSRLSFFSQPMET 420
           ++ V +YQ YMRCQMRQP LETQW LH LP+P SS LAL+LHASS YDSRLSFFSQPMET
Sbjct: 361 VMGVVTYQNYMRCQMRQPPLETQWDLHVLPVPSSSSLALSLHASSAYDSRLSFFSQPMET 420

Query: 421 KPFLAKEEQEKLTCCAECTSNFENEAQHLKSFQSNQVPSWLQQYNANQSHSKDEYVELRR 480
           KPF+AKEE E LTCCAECT+NFENE QHLKSF S QVPSWLQ YN+NQS SKDE VEL+R
Sbjct: 421 KPFIAKEEHEHLTCCAECTTNFENEVQHLKSFHSKQVPSWLQHYNSNQSLSKDELVELKR 480

Query: 481 KWNRFCSSLHRDGSVQSFMAGKSFS-YSSSYPWWPKFHE-SNSISFTDHQTTKPLQSSSF 540
           KWN+ CSSLHRDGSVQS +AGKSFS  SSSYPWWPKFHE S+SISFT HQT K LQSSSF
Sbjct: 481 KWNKLCSSLHRDGSVQS-LAGKSFSCSSSSYPWWPKFHESSSSISFTAHQTPKALQSSSF 540

Query: 541 VPRFRRQQSCTTIEFDFGNATTKHDQGREPSLNSLKHMVGKEVKITLALGSPLFCDSSAD 600
           VPRFRRQQSCTTIEFDFGNA TKH+Q REP L+SLKHMVGKEVKITL LG+ LFCDSSA+
Sbjct: 541 VPRFRRQQSCTTIEFDFGNAATKHEQSREPCLHSLKHMVGKEVKITLELGNSLFCDSSAE 600

Query: 601 SMEMESERNSQRGEILKVLQENVPWQSESLPCVAEAVISAKKNEKSIEWILIEGNDFIGK 660
           SME+ESER SQRGEILK+LQENVPWQ ES+PC+AEAVISAK +EKSI+W+L+EGNDFIGK
Sbjct: 601 SMEIESERKSQRGEILKILQENVPWQLESIPCIAEAVISAKNDEKSIQWVLMEGNDFIGK 660

Query: 661 RKLALAIAESAFGSVDSLLNLNAKSEEMGISRCEMVEKALKSNRELVILVEDVDMADSQF 720
           RK+ +AIAES FGSVD LLNLNAKSE+M ISR E +EKALKSNRELV+LVEDVDM DSQF
Sbjct: 661 RKMGVAIAESVFGSVDFLLNLNAKSEDMEISRSETLEKALKSNRELVVLVEDVDMVDSQF 720

Query: 721 MKFLEDGFESGKLGEAEEETIEKVIFVLTKDDSSDKKKNRA---SSSSVIEMILKIDARA 780
           MK LEDGF+SGKLGE +EE+I+KVIF+LTKDDSSDK KNRA   SSSSVIEM LKI+AR 
Sbjct: 721 MKLLEDGFQSGKLGEVKEESIKKVIFILTKDDSSDKMKNRASSSSSSSVIEMTLKIEARD 780

Query: 781 KPNSDHKRKAEREFENKSKKQRIN-TYEYENQNNRKEFSRQSIINNILDLNLKASNEEEE 840
           + NSDHKRKAE E ENKSK QR N   E +NQ NR +F  Q  I N LDLN+KA  EEEE
Sbjct: 781 ERNSDHKRKAEWEIENKSKNQRTNAALEIKNQKNRTDFRDQPSIKNTLDLNIKAIEEEEE 840

Query: 841 EDEETDQKLPNGQISPISSDLTRETTIYDLKPANGFLESISNRFILNRKPSQESEITEQL 900
           E+E     +PNG+ISP SSDLTRETTI      NGFLESI+NRFILNRK  QESEI E+L
Sbjct: 841 EEE-----VPNGEISPTSSDLTRETTI-----PNGFLESITNRFILNRKSKQESEIREEL 900

Query: 901 RGKMKGAY-KENCKKGNWNWDWDWDWNWDSRFRVEEGVLEGILEGFGSFSNKVFEKWVKE 960
           RG+M  AY KE CKKG        +W WDSRFRVEEGVLE I+EGFGSFS + FEKWVKE
Sbjct: 901 RGEMGEAYNKEKCKKGK-------EWKWDSRFRVEEGVLERIIEGFGSFSKRTFEKWVKE 960

Query: 961 IFQTSLEGGRYGGKGEGGIDIRLCLDQKHILEEEEEEED----GYMGSCLPKKIKLSSM 989
           IFQTSLE GRYGGK   GIDIRLCL+QKH+LEEEEE++D    GYMGSCLPKKI +SS+
Sbjct: 961 IFQTSLESGRYGGK---GIDIRLCLEQKHVLEEEEEDDDDDDNGYMGSCLPKKITVSSI 982

BLAST of Spg017533 vs. ExPASy TrEMBL
Match: A0A6J1ENM0 (protein SMAX1-LIKE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111436146 PE=4 SV=1)

HSP 1 Score: 1314.7 bits (3401), Expect = 0.0e+00
Identity = 735/1022 (71.92%), Postives = 815/1022 (79.75%), Query Frame = 0

Query: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSKP---TLLRRACL 60
           MRSG CA+QQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSS P   TL RRACL
Sbjct: 1   MRSGACASQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSNPSTLTLFRRACL 60

Query: 61  KSHP--PHPLHSRALDLCFNVALNRLPTSPPLLHSPSLSNALIAALKRAQAHQRR--LDH 120
           KSHP  PHPL SRAL+LCFNVALNRLPTSPPLLHSPSLSNALIAALKRAQAHQRR  LDH
Sbjct: 61  KSHPPHPHPLQSRALELCFNVALNRLPTSPPLLHSPSLSNALIAALKRAQAHQRRGSLDH 120

Query: 121 PPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIEEFNSATTP 180
               P     HPLL++KV L HL+ISILDDPSVSRVMREAGFSSTAVKNNIE++ + T P
Sbjct: 121 QHQQPTH--QHPLLAIKVELQHLVISILDDPSVSRVMREAGFSSTAVKNNIEDYTNHTNP 180

Query: 181 I-----SSPLFFSSSSPSLPRNHSSSDAAANFVFELFL-RRSNSNVVVVGDSVGVTEGVV 240
                 +SPLFF  S+PS   + S++D A+ FVFE FL  R   NVVVVGDS+G TEGVV
Sbjct: 181 TFFSPQTSPLFFFPSAPS--ASISNTDNASKFVFEAFLGMRKRRNVVVVGDSIGGTEGVV 240

Query: 241 FEVMRKLKTGEVPHQMKGVKFVELLPPFMGISSSSSPSPSSS---ELRRKLRECCCGSDD 300
            EVMRK KTGEVP +MKGVKFVE L PFMG+ ++ + +  S+    LRRKL E     + 
Sbjct: 241 LEVMRKFKTGEVPEEMKGVKFVEFL-PFMGMLTAGNHNNKSNLCEVLRRKLGE--DSGNG 300

Query: 301 ADNMGVVVYVGDLKWIVEGSNEEVDGLVGEIERLLIKGDSSFNGDGNTFGSNKVKIWLIA 360
               G VVYVGDLKW+VE  ++EVDGL+GEIERLL+ G    N        NKVKIW++ 
Sbjct: 301 GGGGGAVVYVGDLKWVVERDSKEVDGLIGEIERLLVGGFDDHN------PKNKVKIWVMG 360

Query: 361 VASYQVYMRCQMRQPALETQWALHALPLP-SSGLALTLHASSVYDSRLSFFSQPMETKPF 420
           V +YQ YMRCQMRQP LETQW LH LP+P SS LAL+ HASS YDSRLSFFSQPMETKPF
Sbjct: 361 VVTYQNYMRCQMRQPPLETQWDLHVLPVPSSSSLALSPHASSAYDSRLSFFSQPMETKPF 420

Query: 421 LAKEEQEKLTCCAECTSNFENEAQHLKSFQSNQVPSWLQQYNANQSHSKDEYVELRRKWN 480
           +AKEE E LTCCAECT+NFENE QHLKSF S QVPSWLQ YN+NQS SKDE +EL+RKWN
Sbjct: 421 IAKEEHEHLTCCAECTTNFENEVQHLKSFHSKQVPSWLQHYNSNQSLSKDELLELKRKWN 480

Query: 481 RFCSSLHRDGSVQSFMAGKSF---SYSSSYPWWPKFHESN-SISFTDHQTTKPLQSSSFV 540
           + CSSLHRDGSVQS +AGKSF   S SSSYPWWPKFHESN SISFT HQT   LQSSSFV
Sbjct: 481 KLCSSLHRDGSVQS-LAGKSFSCSSSSSSYPWWPKFHESNSSISFTAHQTPNALQSSSFV 540

Query: 541 PRFRRQQSCTTIEFDFGNATTKHDQGREPSLNSLKHMVGKEVKITLALGSPLFCDSSADS 600
           PRFRRQQSCTTIEFDFGNA TKH+Q REP L+SLKHMVGKEVKITLALG+ LFCDSSA+S
Sbjct: 541 PRFRRQQSCTTIEFDFGNAATKHEQSREPCLHSLKHMVGKEVKITLALGNSLFCDSSAES 600

Query: 601 MEMESERNSQRGEILKVLQENVPWQSESLPCVAEAVISAKKNEKSIEWILIEGNDFIGKR 660
           ME+ES R SQRGEILK+LQENVPWQ ES+PC+AEAVIS K +EKSI+W+L+EGNDFIGKR
Sbjct: 601 MEIESVRKSQRGEILKILQENVPWQLESIPCIAEAVISTKNDEKSIQWVLMEGNDFIGKR 660

Query: 661 KLALAIAESAFGSVDSLLNLNAKSEEMGISRCEMVEKALKSNRELVILVEDVDMADSQFM 720
           K+ +AIAES FGSVD LLNLNAKSE+M ISR E +EKALKSNRELV+LVEDVDMADSQFM
Sbjct: 661 KMGVAIAESVFGSVDFLLNLNAKSEDMEISRSETLEKALKSNRELVVLVEDVDMADSQFM 720

Query: 721 KFLEDGFESGKLGEAEEETIEKVIFVLTKDDSSDKKKNRA---SSSSVIEMILKIDARAK 780
           K LEDGF+SGK GE +EE+I+KVIF+LTKDDSSDK KNRA   SSSSVIEM LKI+AR +
Sbjct: 721 KLLEDGFQSGKFGEVKEESIKKVIFILTKDDSSDKMKNRASSSSSSSVIEMTLKIEAREE 780

Query: 781 PNSDHKRKAEREFENKSKKQRIN-TYEYENQNNRKEFSRQSIINNILDLNLKASNEEEEE 840
            N DHKRK+E E ENKSK QR N   E +NQ NR +F  QS I N LDLN+KA  EEE  
Sbjct: 781 HNPDHKRKSEWEIENKSKNQRTNAALEIKNQKNRTDFRDQSSIKNTLDLNIKAIEEEE-- 840

Query: 841 DEETDQKLPNGQISPISSDLTRETTIYDLKPANGFLESISNRFILNRKPSQESEITEQLR 900
                  +PNG+ISP SSDLTRET I      NGFLESI+NRFILNRK  QESEI E+LR
Sbjct: 841 -------VPNGEISPTSSDLTRETAI-----PNGFLESITNRFILNRKSKQESEIREELR 900

Query: 901 GKMKGAY-KENCKKGNWNWDWDWDWNWDSRFRVEEGVLEGILEGFGSFSNKVFEKWVKEI 960
           G+M  AY KE CKKG        +W WDSRFRVEEGVLE I+EGFGSFS + FEKWVKEI
Sbjct: 901 GEMGEAYNKEKCKKGK-------EWKWDSRFRVEEGVLERIIEGFGSFSKRTFEKWVKEI 960

Query: 961 FQTSLEGGRYGGKGEGGIDIRLCLDQKHILEEEEEEE--------DGYMGSCLPKKIKLS 989
           FQTSLE GRYGGK   GIDIRLCL+QKH+LEEEEEEE        DGYMGSCLPKKIK+S
Sbjct: 961 FQTSLESGRYGGK---GIDIRLCLEQKHVLEEEEEEEEDDDDDDNDGYMGSCLPKKIKVS 984

BLAST of Spg017533 vs. ExPASy TrEMBL
Match: A0A0A0LHD2 (Clp R domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G061600 PE=4 SV=1)

HSP 1 Score: 1190.6 bits (3079), Expect = 0.0e+00
Identity = 687/1026 (66.96%), Postives = 787/1026 (76.71%), Query Frame = 0

Query: 1   MRSGT--CAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSKP---TLLRRA 60
           MRS T   ++QQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSKP   +L RRA
Sbjct: 1   MRSVTTCVSSQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSKPSTLSLFRRA 60

Query: 61  CLKSHPPHPLHSRALDLCFNVALNRLPT-SPPLLHSPSLSNALIAALKRAQAHQRR---L 120
           CLKSHPPHPL SRAL+LCFNVALNRLPT SPPLLHSPSLSNALIAALKRAQAHQRR   L
Sbjct: 61  CLKSHPPHPLQSRALELCFNVALNRLPTSSPPLLHSPSLSNALIAALKRAQAHQRRGSSL 120

Query: 121 DHPPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIEEFNS-- 180
           DH          HPLL++KV L HL+ISILDDPSVSRVMREAGFSSTAVKNNIEE++S  
Sbjct: 121 DH----QHQQQQHPLLTIKVELQHLVISILDDPSVSRVMREAGFSSTAVKNNIEEYSSNI 180

Query: 181 ------ATTPISSPLFFSSSSPSLPRNHSSSDAAANFVFELFL-RRSNSNVVVVGDSVGV 240
                  T   ++PLFF   S S     S S+ A+ FVFE+FL  R   NVV+VGDS   
Sbjct: 181 ITTSTATTQTTTTPLFFFPGSGS----SSGSENASKFVFEVFLGMRKRKNVVLVGDS--- 240

Query: 241 TEGVVFEVMRKLKTGEVPHQMKGVKFVELLPPFMGISSSSSPSPSSSELRRKLRECCCGS 300
           +EGVV EVMRK K GEVP +MKGVKFVE +P      ++++ S  S  LRRKL E    +
Sbjct: 241 SEGVVLEVMRKFKMGEVPEEMKGVKFVEFVP-----YNNNNNSNVSEFLRRKLEENYDHT 300

Query: 301 DDADNMGVVVYVGDLKWIVEG---SNEEVDGLVGEIERLLIKGDSSFNGDGNTFGSNKVK 360
           ++ +  GVVVYVGDLKWIVE    SN  VDGLVGEIERLL++G   +N   N     K+K
Sbjct: 301 ENNEG-GVVVYVGDLKWIVERGSCSNFGVDGLVGEIERLLLEG-FHYNDRNNLNIKKKIK 360

Query: 361 IWLIAVASYQVYMRCQMRQPALETQWALHALPLPSSGLALTLHASSVYDSRLSFFSQPME 420
           IW++ VASYQ+YMRCQMR P+LETQW LHALPLPSSGLAL LH+SSVYDSRLSFFSQ ME
Sbjct: 361 IWVMGVASYQIYMRCQMRLPSLETQWDLHALPLPSSGLALALHSSSVYDSRLSFFSQSME 420

Query: 421 TKPF-LAKEEQEKLTCCAECTSNFENEAQHLKSFQSNQVPSWLQQYNANQSHSKDEYVEL 480
           TKPF + KEE + LTCC ECTSNF+NE  HLKSF S Q+PSWL      QS  K+E VEL
Sbjct: 421 TKPFIIGKEEHQNLTCCEECTSNFQNELLHLKSFHSKQLPSWL------QSPPKEELVEL 480

Query: 481 RRKWNRFCSSLHRDGSVQSFMAGKSFSYSSSYPWWPKFHESNSISFTD---HQTTKPLQS 540
           +RKWN+ C++LHRD SVQS + GKSFSYSSSYPWWPK    ++ISFTD   HQT+KPLQ+
Sbjct: 481 KRKWNKLCNTLHRDNSVQS-LIGKSFSYSSSYPWWPK----SNISFTDHHHHQTSKPLQT 540

Query: 541 SSFVPRFRRQQSCTTIEFDFGNATTKHDQGREPSLNSLKHMVGKEVKITLALGSPLFCDS 600
           S+FVPRFRRQQSCTTIEFDFGNA TK +Q  E SLNSLK+M GKEVKITLALG+ LF DS
Sbjct: 541 SNFVPRFRRQQSCTTIEFDFGNAKTKQEQSGELSLNSLKNMDGKEVKITLALGNSLFSDS 600

Query: 601 SADSMEMESERNSQRGEILKVLQENVPWQSESLPCVAEAVISAKKNEKSIEWILIEGNDF 660
           SA+SMEMESER S+RGEILKVL+ENVPW+SE +PC+AEAVIS KK++K I+W+L+EGNDF
Sbjct: 601 SAESMEMESERKSERGEILKVLEENVPWRSELIPCIAEAVISMKKDDKLIQWVLMEGNDF 660

Query: 661 IGKRKLALAIAESAFGSVDSLLNLNAKSEEMGISRCEMVEKALKSNRELVILVEDVDMAD 720
           IGKRK+ + IAE  FGSVD LL+LNAKSEEMGIS+CEM+EKALK N+ELV+LVEDV+MAD
Sbjct: 661 IGKRKMGIVIAELLFGSVDFLLDLNAKSEEMGISKCEMLEKALKLNKELVVLVEDVEMAD 720

Query: 721 SQFMKFLEDGFESGKLGEAEEETIEKVIFVLTKDDSSDKKKNR----------ASSSSVI 780
           SQ MK LE+ F +GK  + +EET++KVIF+LTKD+SSDK KNR          +SSSSVI
Sbjct: 721 SQLMKLLENAFHNGKFEDMKEETVQKVIFILTKDNSSDKMKNRDLWPPQSSSSSSSSSVI 780

Query: 781 EMILKIDARAKPNSDHKRKAEREFENKSKKQRINTYEYENQNNRKEFSRQSIINNILDLN 840
            MILKI+   +PNSDHKRKAE EFENK K QRIN              +QS +NN LDLN
Sbjct: 781 NMILKIE---EPNSDHKRKAEWEFENKPKNQRIN--------------KQSSMNNTLDLN 840

Query: 841 LKASNEEEEEDEETDQKLPNGQIS-PISSDLTRETTIYDLKPANGFLESISNRFILNRKP 900
           +KA +EEEEE+EE      NG IS PI+SDLT ETT+      NGF+ESI NRF++N+K 
Sbjct: 841 IKAEDEEEEEEEE-----ENGGISTPITSDLTGETTL-----PNGFMESIRNRFVMNKKA 900

Query: 901 SQESEITEQLRGKMKGAYKENCKKGNWNWDWDWDWNWDSRFRVEEGVLEGILEGFGSFSN 960
            QES I E+L GKM+ AYKE CK             WDSRFRVEEGV+E ILEGFGSFS 
Sbjct: 901 KQESGIREELVGKMREAYKEKCK-------------WDSRFRVEEGVIERILEGFGSFSK 952

Query: 961 KVFEKWVKEIFQTSLEGGRYGGKGEGGID-IRLCLDQKHILEEEEEEEDGYMGSCLPKKI 990
           ++FEKWVKEIFQTSLE GRYGGKGEGGID I LCLD KHIL     EEDGYMGSCLPKKI
Sbjct: 961 RMFEKWVKEIFQTSLENGRYGGKGEGGIDIINLCLDHKHIL-----EEDGYMGSCLPKKI 952

BLAST of Spg017533 vs. TAIR 10
Match: AT4G29920.1 (Double Clp-N motif-containing P-loop nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 546.6 bits (1407), Expect = 4.1e-155
Identity = 426/1089 (39.12%), Postives = 583/1089 (53.54%), Query Frame = 0

Query: 1    MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLL-SSKPTLLRRACLKS 60
            MR+G     QTLTPEAASVLK SL+LA RRGHS VTPLHVASTLL SS+  L RRACLKS
Sbjct: 1    MRTGAYTVHQTLTPEAASVLKQSLTLARRRGHSQVTPLHVASTLLTSSRSNLFRRACLKS 60

Query: 61   HP--------PHP-LHSRALDLCFNVALNRLPTSP-PLLHS-PSLSNALIAALKRAQAHQ 120
            +P         HP LH RAL+LCFNV+LNRLPT+P PL  + PSLSNAL+AALKRAQAHQ
Sbjct: 61   NPFTALGRQMAHPSLHCRALELCFNVSLNRLPTNPNPLFQTQPSLSNALVAALKRAQAHQ 120

Query: 121  RRLDHPPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIEEFN 180
            RR         S  + P L++KV L  L++SILDDPSVSRVMREAG SS +VK+NIE+ +
Sbjct: 121  RR--GCVEQQQSQQNQPFLAVKVELEQLVVSILDDPSVSRVMREAGLSSVSVKSNIEDDS 180

Query: 181  SATTPI------SSPLFFSSSSPS----------------------LPRNHS-------- 240
            S  +P+      S  +F S  SPS                      L  +HS        
Sbjct: 181  SVVSPVFYGSSSSVGVFSSPCSPSSSENNQGGGTLSPNPSKIWHAHLTNHHSFEQNPFFH 240

Query: 241  -------SSDAA------ANFVFELFLRRSNS---NVVVVGDSVGVTEGVVFEVMRKLKT 300
                   + D A      AN V E+ L + N+   N V+VGDSV +TEGVV ++M +++ 
Sbjct: 241  FPKGKTFTPDQAFPVREDANPVIEVLLGKKNNKKRNTVIVGDSVSLTEGVVAKLMGRIER 300

Query: 301  GEVPHQMKGVKFVELLPPFMGISSSSSPSPSSS--ELRRKLRECCCGSDDADNMGVVVYV 360
            GEVP  +K   F++     +G++            EL+RK+             GV+V +
Sbjct: 301  GEVPDDLKQTHFIKFQFSQVGLNFMKKEDIEGQVRELKRKIDSFTSWG----GKGVIVCL 360

Query: 361  GDLKWIVEGSNEE--------VDGLVGEIERLLIKGDSSFNGDGNTFGSNKVKIWLIAVA 420
            GDL W V G             D LV EI RL+             + +   K+WL+  A
Sbjct: 361  GDLDWAVWGGGNSASSSNYSAADHLVEEIGRLVY-----------DYSNTGAKVWLLGTA 420

Query: 421  SYQVYMRCQMRQPALETQWALHALPLPSSGLALTLHASSVYDSRLSFFSQPMETKPFLAK 480
            SYQ YMRCQM+QP L+  WAL A+ +PS GL+LTLHASS         SQ ME KPF  K
Sbjct: 421  SYQTYMRCQMKQPPLDVHWALQAVSIPSGGLSLTLHASS-----SEMASQVMEMKPFRVK 480

Query: 481  EEQE---------KLTCCAECTSNFENEAQHLKSFQSNQVPSWLQQYNANQS-HSKDEYV 540
            EE+E         KL  C EC  N+E EA+   S Q   +P WLQ +  N + + KDE  
Sbjct: 481  EEEEGAREEEEEDKLNFCGECAFNYEKEAKAFISAQHKILPPWLQPHGDNNNINQKDELS 540

Query: 541  ELRRKWNRFCSSLHRDGSVQSFMAGKSFSYSSSYPWWPKFHESNSISFTDHQTTKPLQSS 600
             LR+KWNRFC +LH      S  A ++   SS  P           S  D    +  ++S
Sbjct: 541  GLRKKWNRFCQALHH--KKPSMTAWRAEQSSSVLPG----------SLMDSSLKQNSRAS 600

Query: 601  SFVPRFRRQQSCTTIEFDFG-NATTKHDQGREPSLNSLK--HMVGKEVKITLALGSPLFC 660
            S V +FRRQ SC TIEF FG N      +  E SL+  K  +  G + KITLALG   F 
Sbjct: 601  SSVAKFRRQNSC-TIEFSFGSNRQEGLKKTDELSLDGFKSNNDEGVKTKITLALGHSPFP 660

Query: 661  DSSADSMEMESERNSQRGEILKVLQENVPWQSESLPCVAEAV-ISAKKNEKSIEWILIEG 720
              S +S E E E+  +  ++L+ L EN+PWQ + LP + EA+  S K++++   W+L+ G
Sbjct: 661  SDSENSEEEEPEKAIKMSKLLEKLHENIPWQKDVLPSIVEAMEESVKRSKRKDAWMLVSG 720

Query: 721  NDFIGKRKLALAIAESAFGSVDSLLNLNAKSEEMGISRCEMVEKALKSNRELVILVEDVD 780
            ND   KR+LA+ +  S FGS +++L +N ++ +     CE ++ ALK   E+VIL+E VD
Sbjct: 721  NDVTAKRRLAITLTTSLFGSHENMLKINLRTSKAS-EACEELKNALKKKEEVVILIERVD 780

Query: 781  MADSQFMKFLEDGFESGKLGEAEEETIEKVIFVLTKDDSSDKKKNRASSSSVIEMILKID 840
            +AD+QFM  L D FE+G L +  +    ++IF+LT++D    +    +   VI M+L  +
Sbjct: 781  LADAQFMNILVDRFEAGDL-DGFQGKKSQIIFLLTREDDECVE----NEHFVIPMVLNCN 840

Query: 841  ARAKPNSDHKRKAEREFEN---KSKKQRINTYEYEN------QNNRKEFSRQ-SIINNIL 900
                   ++KRK E +      K K  RI   + E+       N +KEFSRQ    +N L
Sbjct: 841  KSGSGLVNNKRKPEYDAAPTMIKKKNPRIEEDDDESNVACDISNIKKEFSRQLKFESNAL 900

Query: 901  DLNLKASNEEEEEDEETDQKLPNGQISPISSDLTRETTIYDLKPANGFLESISNRFILNR 960
            DLNL+   +E+EE+E           + ISS                FL+SI NRF  + 
Sbjct: 901  DLNLRVDADEDEEEEAK-------PATEISSGFEER-----------FLDSIQNRF--DF 960

Query: 961  KPSQESEITEQLRGKMKGAYKENCKKGNWNWDWDWDWNWDSRFRVEEGVLEGILEGFGSF 990
                + +IT+    K+K + +E   +    +           F V+  ++E   +G G F
Sbjct: 961  TVLSDEDITKFFVTKIKDSCEEILGQREERFG----------FTVDAELIEKFYKGCGFF 1017

BLAST of Spg017533 vs. TAIR 10
Match: AT5G57130.1 (Clp amino terminal domain-containing protein )

HSP 1 Score: 510.4 bits (1313), Expect = 3.3e-144
Identity = 398/1102 (36.12%), Postives = 574/1102 (52.09%), Query Frame = 0

Query: 1    MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSKPTLLRRACLKSH 60
            MR+G    QQTLT EAASVLKHSL+LA RRGH+ VTPLHVA+TLLSS+ +LLRRAC+KSH
Sbjct: 1    MRTGGYTIQQTLTTEAASVLKHSLTLARRRGHAQVTPLHVAATLLSSRTSLLRRACIKSH 60

Query: 61   P---------------------PHPLHSRALDLCFNVALNRLPTSP-PLLH-SPSLSNAL 120
            P                      HPL  RAL+LCFNVALNRLPT P P+ H  PSL+NAL
Sbjct: 61   PGFSTNYQFAPSRLQHHHHHNQNHPLQCRALELCFNVALNRLPTVPGPMFHGQPSLANAL 120

Query: 121  IAALKRAQAHQRR----LDHPPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREA 180
            +AALKRAQAHQRR            P      LL++KV L  L+ISILDDPSVSRVMREA
Sbjct: 121  VAALKRAQAHQRRGCIEQQQQTQTHPQTQQTQLLAVKVELEQLVISILDDPSVSRVMREA 180

Query: 181  GFSSTAVKNNIEE---------------FNSAT--------------------------- 240
            GF+STAVK+ +E+               F+S                             
Sbjct: 181  GFNSTAVKSCVEDCSVSSVFYGGSAVGVFSSPNSPDQQQQHHNSINRLHHYQNPKDFNFI 240

Query: 241  --------------TPISSPLFFSSSSPSLPRNHSSSDAAANFVFELFLRR--SNSNVVV 300
                          +P  +PL  SSS+    +     +     V ++ +R+     N V+
Sbjct: 241  NPNFPLWQTHFLNQSPDQNPLLLSSSASHHHQQQRLREIDLKLVVDVLMRKKTKKKNPVI 300

Query: 301  VGDSVGVTEGVVFEVMRKLKTGEVPH--QMKGVKFVE--LLPPFMGISSSSSPSPSSSEL 360
            VGDS+  TEG V E+M KL+ GE+    ++K   FV+    P             +  EL
Sbjct: 301  VGDSISFTEGFVSELMAKLERGEIDQTGELKQTHFVKFHFSPMASKFMRREDVELNIKEL 360

Query: 361  RRKLRECCCGSDDADNMGVVVYVGDLKWIVE--------GSNE------EVDGLVGEIER 420
            R+K+        +A     +++ GDLKW V+        G NE       +D LV EI +
Sbjct: 361  RKKVLSLTTSGKNA-----IIFTGDLKWTVKEITNNNSGGINEISSSYSPLDHLVEEIGK 420

Query: 421  LLIKGDSSFNGDGNTFGSNKVKIWLIAVASYQVYMRCQMRQPALETQWALHALPLPSS-G 480
            L+    +  N DG+       K+W++  AS+Q YMRCQMRQP+LET WALH + +PSS  
Sbjct: 421  LI----TECNDDGDDDDCKTRKVWVMGTASFQTYMRCQMRQPSLETLWALHPVSVPSSAN 480

Query: 481  LALTLHASSVYDSR-LSFFSQPMETKPFLAKEEQEK----LTCCAECTSNFENEAQHLKS 540
            L L+LHA+S +++R +S  +       +   EE+E     L+CC EC ++F+ EA+ LK+
Sbjct: 481  LGLSLHATSGHEARNMSTVNATKSLSGYDKAEEEETISHVLSCCPECVTSFDREAKSLKA 540

Query: 541  FQSNQVPSWLQQYNANQSHSKDEYVELRRKWNRFCSSLHRDGSVQSFMAGKSFSYSSSYP 600
             Q   +PSWLQ ++A+ S  KDE + L+RKWNRFC +LH      S M     +Y    P
Sbjct: 541  NQDKLLPSWLQSHDADSSSQKDELMGLKRKWNRFCETLHNQTGQLSMMG----NYPYGLP 600

Query: 601  WWPKFHESNSISFTDHQTTKPLQ-SSSFVPRFRRQQSCTTIEFDFGNATTKHDQGREPSL 660
            +      S S S  D    KP Q +++ + +FRRQ SC TIEFD G    +H++G   S+
Sbjct: 601  YGSSHESSKSTSLIDSLGLKPNQRATNSIAKFRRQNSC-TIEFDLGG--NEHEKGE--SI 660

Query: 661  NSLKHMVGKEVKITLALGSPLFCDSSADSMEMESERNSQRGEILKVLQENVPWQSESLPC 720
            N  +   G E  +TL LG  LF   S       ++   +   ++K L+E++P Q+ ++  
Sbjct: 661  NEAEDDKGNET-VTLDLGRSLFRSDSV------TDTRLKLSALVKALEESIPRQTVTMRL 720

Query: 721  VAEAVISAKKNEKSIEWILIEGNDFIGKRKLALAIAESAFGSVDSL--LNLNAKSEEMGI 780
            +AE+++     +K   WI+IEG D   KR++A  ++ES FGS +SL  ++L  K  E   
Sbjct: 721  IAESLMDCVSKKKD-SWIIIEGRDTTAKRRVARTVSESVFGSFESLVHIDLKKKGNESKA 780

Query: 781  SRCEMVEKALKSNRELVILVEDVDMADSQFMKFLEDGFESGKLGEAEEETIEKVIFVLTK 840
            S   ++   LK+  ++V L+ED+D+ADS+F+K L D FE  +  +   +   + IF+LTK
Sbjct: 781  SPATLLAYELKNPEKVVFLIEDIDLADSRFLKLLADRFEDKRRIKTGIDH-RQAIFILTK 840

Query: 841  DDSSDKKKNRASSSSVIEMILKIDARAKPNSDHKRKAEREFENKSKKQRINTYEYENQNN 900
            +DS    +N  +  SV+++ L+I A++ P    K +++   EN    +            
Sbjct: 841  EDS----RNVRNRDSVLQIGLEITAQS-PGKKRKPESDLSIENGFWMK------------ 900

Query: 901  RKEFSRQSIIN-NILDLNLKASNEEEEEDEETDQKLPNGQISPISSDLTRETTIYDLKPA 960
            ++  SRQS  N + LDLN+KA +EE E           G+ISPISSDLT E    +   +
Sbjct: 901  KEVCSRQSSFNSSYLDLNIKAEDEEVE-----------GEISPISSDLTGEEET-EFSSS 960

Query: 961  NGFLESISNRFILNRKPSQESEITEQLRGKMKGAYKENCKKGNWNWDWDWDWNWDSRFRV 990
            + FL  I NRF+LNR      E     +G +  A++E           + +     RF V
Sbjct: 961  SNFLNRIQNRFVLNRSCEPGIE-----KGMITAAFREIFP--------EREEGGGVRFSV 1020

BLAST of Spg017533 vs. TAIR 10
Match: AT3G52490.1 (Double Clp-N motif-containing P-loop nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 323.6 bits (828), Expect = 5.7e-88
Identity = 285/849 (33.57%), Postives = 412/849 (48.53%), Query Frame = 0

Query: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSKPTLLRRACLKSH 60
           MR+G C  +Q LT +AA+V+K ++ LA RRGH+ VTPLHVAST+LS+   LLR ACL+SH
Sbjct: 1   MRAGGCTVEQALTADAANVVKQAMGLARRRGHAQVTPLHVASTMLSAPTGLLRTACLQSH 60

Query: 61  PPHPLHSRALDLCFNVALNRLPTSP-------PLLHSPSLSNALIAALKRAQAHQRRLDH 120
             HPL  RAL+LCFNVALNRLPTS        P    PS+SNAL AA KRAQAHQRR   
Sbjct: 61  -THPLQCRALELCFNVALNRLPTSTGSPMLGVPTSPFPSISNALGAAFKRAQAHQRR--- 120

Query: 121 PPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIE-----EFN 180
                      P+L++K+ +  LIISILDDPSVSRVMREAGFSS  VK  +E     E  
Sbjct: 121 ---GSIESQQQPILAVKIEVEQLIISILDDPSVSRVMREAGFSSPQVKTKVEQAVSLEIC 180

Query: 181 SATTPISSPLFFSSSSPSLPRNHSSSDAAANFVFELFLRRSNSNVVVVGDSVGVTEGVVF 240
           S TT  S P      +P   RN    +   N V      +   N V+VG+ +   +GVV 
Sbjct: 181 SKTTSSSKPKEGKLLTP--VRNEDVMNVINNLV-----DKKRRNFVIVGECLATIDGVVK 240

Query: 241 EVMRKLKTGEVPHQMKGVKFVELLPPFMGISSSSSPSPSSSELRRKLRECCCGSDDADNM 300
            VM K+   +VP  +K VKF+ L       S SS   PS +++ RKL E           
Sbjct: 241 TVMEKVDKKDVPEVLKDVKFITL-------SFSSFGQPSRADVERKLEELETLVKSCVGK 300

Query: 301 GVVVYVGDLKWIVE---------GSNEE---VDGLVGEIERL---LIKGDSSFNGDGNTF 360
           GV++ +GDL W VE          +N+    V+ ++ EI +L   L+ GD          
Sbjct: 301 GVILNLGDLNWFVESRTRGSSLYNNNDSYCVVEHMIMEIGKLACGLVMGDHG-------- 360

Query: 361 GSNKVKIWLIAVASYQVYMRCQMRQPALETQWALHALPLPSSGLALTLHASSVYDSRLSF 420
                + WL+ +A+ Q Y+RC+  QP+LE+ W L  L +P++  +L L  S V +S L  
Sbjct: 361 -----RFWLMGLATSQTYVRCKSGQPSLESLWCLTTLTIPATSNSLRL--SLVSESELEV 420

Query: 421 FSQPMETKPFLAKEEQEKLTCCAECTSNFENEAQHLKSFQSN----QVPSWLQQY---NA 480
             +  E      ++  ++L+ C EC+  FE+EA+ LKS  SN     +P+WLQQY   N 
Sbjct: 421 --KKSENVSLQLQQSSDQLSFCEECSVKFESEARFLKSSNSNVTTVALPAWLQQYKKENQ 480

Query: 481 NQSHSKDEYVELRRKWNRFCSSLHRDGSVQSF-MAGKSFSYSSSYPWWPKFHESNSISFT 540
           N     D   EL  KWN  C S+H+  S+++  ++  + S+S S           SIS  
Sbjct: 481 NSHTDSDSIKELVVKWNSICDSIHKRPSLKTLTLSSPTSSFSGS--------TQPSISTL 540

Query: 541 DHQTTKPLQSSSFVPRFRRQQSCTTIEFDFGNATTKHDQGREPS-LNSLKHMVGKEVKIT 600
            H     LQ++   P          IE    N    H    E S L         E K  
Sbjct: 541 HH-----LQTNGDWP---------VIE---TNTHRHHSVVHETSHLRLFIPEHDSEQKTE 600

Query: 601 LALGSPLFC----DSSADSMEME------SERNSQR-GEILKVLQENVPWQSESLPCVAE 660
           L   +P        SS+D+ME+E       E N++    +   L+  VPWQ + +P +A+
Sbjct: 601 LVCSNPNSTMNSEASSSDAMELEHASSRFKEMNAENLATLCAALESKVPWQKDLVPELAK 660

Query: 661 AV-----------ISAKKNEKSIEWILIEGNDFIGKRKLALAIAESAFGSVDSLLNLNAK 720
            V           I+  +++K   W+  +G D   K K+A  +A+  FGS DS +++   
Sbjct: 661 TVLKCRSGSSTRKINGNEDKKEDTWMFFQGLDVDAKEKIARELAKLVFGSQDSFVSICLS 720

Query: 721 S------------------EEMGISRCEMVEKALKSNRELVILVEDVDMADSQFMKFLED 754
           S                  +E  +S  E   +A+  +   VILVED++ AD       + 
Sbjct: 721 SFSSTRSDSAEDLRNKRLRDEQSLSYIERFSEAVSLDPNRVILVEDIEQADYLSQVGFKR 780

BLAST of Spg017533 vs. TAIR 10
Match: AT5G57710.1 (Double Clp-N motif-containing P-loop nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 272.7 bits (696), Expect = 1.2e-72
Identity = 253/806 (31.39%), Postives = 379/806 (47.02%), Query Frame = 0

Query: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSKPTLLRRACLKSH 60
           MR+G    QQTLTPEAA+VL  S++ AARR H   TPLHVA+TLL+S    LRRAC++SH
Sbjct: 1   MRAGLSTIQQTLTPEAATVLNQSIAEAARRNHGQTTPLHVAATLLASPAGFLRRACIRSH 60

Query: 61  P--PHPLHSRALDLCFNVALNRLPTSPPLL-HSPSLSNALIAALKRAQAHQRRLDHPPPP 120
           P   HPL  RAL+LCF+VAL RLPT+     + P +SNAL+AALKRAQAHQRR       
Sbjct: 61  PNSSHPLQCRALELCFSVALERLPTATTTPGNDPPISNALMAALKRAQAHQRR------G 120

Query: 121 PPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNIEE-FNSATTPISS 180
            P     PLL++KV L  LIISILDDPSVSRVMREA FSS AVK  IE+  N++ TP   
Sbjct: 121 CPEQQQQPLLAVKVELEQLIISILDDPSVSRVMREASFSSPAVKATIEQSLNNSVTPTPI 180

Query: 181 PLFFSSS-----------------SPSLPRNHSSSDAAAN------FVFELFLRRSNSNV 240
           P   S                   +P L +N SS  +  +       V ++  R    N 
Sbjct: 181 PSVSSVGLNFRPGGGGPMTRNSYLNPRLQQNASSVQSGVSKNDDVERVMDILGRAKKKNP 240

Query: 241 VVVGDSVGVTEGVVFEVMRKLKTGEVPH-QMKGVKFVELLPPFMGISSSSSPSPSSSELR 300
           V+VGDS      V+ E+++K++ GEV +  +K  K V L         SS  +    EL 
Sbjct: 241 VLVGDS--EPGRVIREILKKIEVGEVGNLAVKNSKVVSL------EEISSDKALRIKELD 300

Query: 301 RKLRECCCGSDDADNMGVVVYVGDLKWIVEGSN----------EEVDGLVGEIERLLIKG 360
             L+     SD     GV++ +GDLKW+VE  +          E     V E+ RLL K 
Sbjct: 301 GLLQTRLKNSDPIGGGGVILDLGDLKWLVEQPSSTQPPATVAVEIGRTAVVELRRLLEK- 360

Query: 361 DSSFNGDGNTFGSNKVKIWLIAVASYQVYMRCQMRQPALETQWALHALPLPSSGLALTLH 420
              F G          ++W I  A+ + Y+RCQ+  P++ET W L A+      +A    
Sbjct: 361 ---FEG----------RLWFIGTATCETYLRCQVYHPSVETDWDLQAV-----SVAAKAP 420

Query: 421 ASSVYDSRLSFFSQPMETKPFLAKEEQEKLTCCAECTSNFENEAQHLKSF---------- 480
           AS V+    +        K F+       L CC +C  ++E E   + S           
Sbjct: 421 ASGVFPRLANNLESFTPLKSFV--PANRTLKCCPQCLQSYERELAEIDSVSSPEVKSEVA 480

Query: 481 QSNQVPSWLQQYNANQSHSKDEYVELRRKWNRFCSSLH--------RDGSVQSFMAGKSF 540
           Q  Q+P WL +        + +  E+++KWN  C  LH        R   +   +   + 
Sbjct: 481 QPKQLPQWLLKAKPVDRLPQAKIEEVQKKWNDACVRLHPSFHNKNERIVPIPVPITLTTS 540

Query: 541 SYSSS----YPWWPKFHESNSISFTDHQTTKPLQSSSFVPRFRRQQSCTTIEFD--FGNA 600
            YS +     P  PK   +  +    H   KP+         ++    + ++ D   G A
Sbjct: 541 PYSPNMLLRQPLQPKLQPNRELRERVH--LKPMSPLVAEQAKKKSPPGSPVQTDLVLGRA 600

Query: 601 TTKHDQGREPSLNSLKHMVGKEVKITLALGSPLFCDSSADSMEMESERNSQRGEILKVLQ 660
                 G     + L  +  + V+    + S L  ++  +S++++  +     ++LK + 
Sbjct: 601 EDSEKAGDVQVRDFLGCISSESVQNNNNI-SVLQKENLGNSLDIDLFK-----KLLKGMT 660

Query: 661 ENVPWQSESLPCVAEAVISAKKN--------EKSIEWILIEGNDFIGKRKLALAIAESAF 720
           E V WQ+++   VA  V   K           K   W+L  G D +GKRK+  A++   +
Sbjct: 661 EKVWWQNDAAAAVAATVSQCKLGNGKRRGVLSKGDVWLLFSGPDRVGKRKMVSALSSLVY 720

Query: 721 GSVDSLLNLNAK-------SEEMGISRCEMVEKALKSNRELVILVEDVDMADSQFMKFLE 728
           G+   ++ L ++       S   G +  + + + +K +   VIL+ED+D AD      ++
Sbjct: 721 GTNPIMIQLGSRQDAGDGNSSFRGKTALDKIAETVKRSPFSVILLEDIDEADMLVRGSIK 763

BLAST of Spg017533 vs. TAIR 10
Match: AT4G30350.1 (Double Clp-N motif-containing P-loop nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 242.3 bits (617), Expect = 1.7e-63
Identity = 279/1005 (27.76%), Postives = 431/1005 (42.89%), Query Frame = 0

Query: 1   MRSGTCAAQQTLTPEAASVLKHSLSLAARRGHSHVTPLHVASTLLSSKPTLLRRACLKSH 60
           MR+     QQTLTPEAA+VL  S++ A RR H H TPLHVA+TLLSS    LR+AC+KSH
Sbjct: 1   MRADLITIQQTLTPEAATVLNQSIAEATRRNHGHTTPLHVAATLLSSSSGYLRQACIKSH 60

Query: 61  P--PHPLHSRALDLCFNVALNRLPT--------------SPPLLHSPSLSNALIAALKRA 120
           P   HPL  RAL+LCF+VAL RLPT              SP     P LSNAL AALKRA
Sbjct: 61  PNSSHPLQCRALELCFSVALERLPTTSTTTTTTSSSSSSSPSQTQEPLLSNALTAALKRA 120

Query: 121 QAHQRRLDHPPPPPPSPPHHPLLSLKVHLHHLIISILDDPSVSRVMREAGFSSTAVKNNI 180
           QAHQRR        P     PLL++KV L  LIISILDDPSVSRVMREA FSS AVK+ I
Sbjct: 121 QAHQRR------GCPEQQQQPLLAVKVELEQLIISILDDPSVSRVMREASFSSPAVKSAI 180

Query: 181 EE---------------------------FNSATTPISSPLFFSS--SSPSLPRNHS--- 240
           E+                           + S   P++  L+ +     P +        
Sbjct: 181 EQSLIGNSVSNSRQTGSPGIINPSAIGFGYRSVPAPVNRNLYLNPRLQQPGVGMQSGMMI 240

Query: 241 SSDAAANFVFELFLRRSNSNVVVVGDSVGVTEGVVFEVMRKLKTGEVPH-QMKGVKFVEL 300
                A  V E+ +R    N V+VGDS      +V E++ K++ GE     ++  + + L
Sbjct: 241 QRTDEAKRVIEIMIRTRKRNPVLVGDS--EPHILVKEILEKIENGEFSDGALRNFQVIRL 300

Query: 301 LPPFMGISSSSSPSPSSSELRRKLRECC-CGSDDADNMGVVVYVGDLKWIVEGSNEEVDG 360
               +            S+L  +L E            GVV+ +GDLKW+VE       G
Sbjct: 301 EKELV------------SQLATRLGEISGLVETRIGGGGVVLDLGDLKWLVEHPAAN-GG 360

Query: 361 LVGEIERLLIKGDSSFNGDGNTFGSNKVKIWLIAVASYQVYMRCQMRQPALETQWALHAL 420
            V E+ +LL +               K ++  I  A+ + Y+RCQ+  P++E  W L A+
Sbjct: 361 AVVEMRKLLER--------------YKGRLCFIGTATCETYLRCQVYYPSMENDWDLQAI 420

Query: 421 PL--PSSGLALTLHASSVYDSRLSFFSQPM-------ETKPFLAKEEQEKLTCCAECTSN 480
           P+   SS  A+     S  ++     S  +        T+ F  +    K++CC+ C  +
Sbjct: 421 PIAAKSSLPAIFPRLGSNNNNNAMLLSNNIISIESISPTRSF--QIPMSKMSCCSRCLQS 480

Query: 481 FENEA----QHLKSFQSNQVPSWLQQYNANQSHSK-----DEYVELRRKWNRFCSSLHRD 540
           +EN+     + L     + +P WLQ   AN    K      + VEL++KWN  C  LH +
Sbjct: 481 YENDVAKVEKDLTGDNRSVLPQWLQNAKANDDGDKKLTKDQQIVELQKKWNDLCLRLHPN 540

Query: 541 GSVQSFMAGKSFSYSSSYPWWPKFHESNSISFTDHQTTKPLQSSSFVPRFRRQQSCTTIE 600
            SV   +A  + S         K +  + I+        P+ +   + R  R  S     
Sbjct: 541 QSVSERIAPSTLSMM-------KINTRSDIT----PPGSPVGTDLVLGRPNRGLS----- 600

Query: 601 FDFGNATTKHDQGREPSLNSLKHMVGKEVKITLALGSPLFCDSSADSMEMESERNSQRGE 660
                          P   + +   GK       LG         DS +++  +     +
Sbjct: 601 --------------SPEKKTREARFGK-------LG---------DSFDIDLFK-----K 660

Query: 661 ILKVLQENVPWQSESLPCVAEAVISAKK---NEKSIEWILIEGNDFIGKRKLALAIAESA 720
           +LK L ++V WQ ++   VA A+   K      K   W++  G D  GK K+A A+++  
Sbjct: 661 LLKGLAKSVWWQHDAASSVAAAITECKHGNGKSKGDIWLMFTGPDRAGKSKMASALSDLV 720

Query: 721 FGSVDSLLNLNAKSEE------MGISRCEMVEKALKSNRELVILVEDVDMADSQFMKFLE 780
            GS    ++L + S         G +  +   +A++ N   VI++ED+D AD      ++
Sbjct: 721 SGSQPITISLGSSSRMDDGLNIRGKTALDRFAEAVRRNPFAVIVLEDIDEADILLRNNVK 780

Query: 781 DGFESGKLGEA--EEETIEKVIFVLTKDDSSDKKKNRASSSSVIEMILKIDARAKPNSDH 840
              E G++ ++   E ++  VI +LT + S    KN AS       I +    +  N   
Sbjct: 781 IAIERGRICDSYGREVSLGNVIIILTANSSLGSAKNVAS-------IDETRLESLVNKGW 840

Query: 841 KRKAEREFENKSKKQRINTYEYENQNNRKEFSRQSIINNILDLNLKASNEEEEEDEETDQ 900
           + +      +K++K++ N    +N   ++       +N   + +  +S+   E D+E + 
Sbjct: 841 ELRLSVCNSSKTRKRKPNWLYSDNDQTKQRKEICFDLNEAAEFD-SSSDVTVEHDQEDNG 890

Query: 901 KLPNGQISPISSDLTRETTIYDLKPANGFLESISNRFILNRKPSQESEITEQLRGKMKGA 927
            L +  +  +   +      +D    +   ES+  RF          EI +    ++ GA
Sbjct: 901 NLVHKLVGLVDDAILFRPVDFD-SIKSKTAESLKKRFSNGLADGLTVEIEDDALERIAGA 890