Spg017085 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg017085
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRNase H domain-containing protein
Locationscaffold4: 34974916 .. 34975940 (+)
RNA-Seq ExpressionSpg017085
SyntenySpg017085
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGTATGTAGAGAAATCTCTACATATCGGTATGCAGTGAAGTTTTTTTCCTATTAATTGTGGTGTGTAGGAAGGGAAAATTTCTAGTACTACGGTAGTATGGAAAATTTTCCATACTACCTCAATTCATTGTTTGCCATGAAAGAAATTTTTAAGAATTATGTGGGACCCATGGAATATAATATTATATTATTAAATGCTCATTAAATGACGTGTCAAATACTGAATGGAGGAAGTAGGTACTACCGTAGTATTAGAAATTTTCCCTGTGTAGGAAGATATCTACACACATGGAGCTAAATATTATTTATTAATTTTTGGGCCGGTTGCAAGGATGGCAAACTGGGCCAAACTAATTACAAATATAGCAGCCCACAAAAAAAAATTGCAAAAATAGCAAAAATTGAAGGTATGAAACGCGCATTTACTAAAATACCCTTCCTTCCTAAACCCTGAACCCTATCTTCATTTTCTATCAACGACATTTCATTTTCTATCATCTCCGTTTCAAATTTGCTCGATTTCTGAATTGGTGCGGAAAAGGCAGATTGGGGTGGGTCATTCGTGACTCCTCAGGATCTTTAATTGGTGTAGGCTGCAAGCGAGTTCAAAGGAAATGGTCAATTACGGCCCTAGAAGGGGAAGCCATGCGTGAAGGGTTGGAAGCTTACCTGAAAAGCTACAGCAGCAGCAATCCCAGACCTCCCTTGGCAATTGAGTCTGATTCGATTAGCCTGATCAAGTTGATTTCGAGAGAAGACGAGGATCTGACAGAAGCGGGTTTGATCGCAGAGTTGATCGTCAATCTGGCAAGGAACACATCGATTTCTTTCTCTCACTGTAGGCGTGAGTGTAATTTTGTAGCCCACCAGGTTGCTCGAGCAGCAGCTGAAAATGGGGATTATTTTTGTGTTTTAGGTCGTTCTTCTTCCCCTCTTCTGGAAGATGAAGGTGTTGGTGGGGGTGCTTGTTTCCCCTCATGGTTTGTATCTGTTTTAGCGAACAGTTGTTTTGGTGGTTGA

mRNA sequence

ATGGTGGTATGTAGAGAAATCTCTACATATCGATTGGGGTGGGTCATTCGTGACTCCTCAGGATCTTTAATTGGTGTAGGCTGCAAGCGAGTTCAAAGGAAATGGTCAATTACGGCCCTAGAAGGGGAAGCCATGCGTGAAGGGTTGGAAGCTTACCTGAAAAGCTACAGCAGCAGCAATCCCAGACCTCCCTTGGCAATTGAGTCTGATTCGATTAGCCTGATCAAGTTGATTTCGAGAGAAGACGAGGATCTGACAGAAGCGGGTTTGATCGCAGAGTTGATCGTCAATCTGGCAAGGAACACATCGATTTCTTTCTCTCACTGTAGGCGTGAGTGTAATTTTGTAGCCCACCAGGTTGCTCGAGCAGCAGCTGAAAATGGGGATTATTTTTGTGTTTTAGGTCGTTCTTCTTCCCCTCTTCTGGAAGATGAAGGTGTTGGTGGGGGTGCTTGTTTCCCCTCATGGTTTGTATCTGTTTTAGCGAACAGTTGTTTTGGTGGTTGA

Coding sequence (CDS)

ATGGTGGTATGTAGAGAAATCTCTACATATCGATTGGGGTGGGTCATTCGTGACTCCTCAGGATCTTTAATTGGTGTAGGCTGCAAGCGAGTTCAAAGGAAATGGTCAATTACGGCCCTAGAAGGGGAAGCCATGCGTGAAGGGTTGGAAGCTTACCTGAAAAGCTACAGCAGCAGCAATCCCAGACCTCCCTTGGCAATTGAGTCTGATTCGATTAGCCTGATCAAGTTGATTTCGAGAGAAGACGAGGATCTGACAGAAGCGGGTTTGATCGCAGAGTTGATCGTCAATCTGGCAAGGAACACATCGATTTCTTTCTCTCACTGTAGGCGTGAGTGTAATTTTGTAGCCCACCAGGTTGCTCGAGCAGCAGCTGAAAATGGGGATTATTTTTGTGTTTTAGGTCGTTCTTCTTCCCCTCTTCTGGAAGATGAAGGTGTTGGTGGGGGTGCTTGTTTCCCCTCATGGTTTGTATCTGTTTTAGCGAACAGTTGTTTTGGTGGTTGA

Protein sequence

MVVCREISTYRLGWVIRDSSGSLIGVGCKRVQRKWSITALEGEAMREGLEAYLKSYSSSNPRPPLAIESDSISLIKLISREDEDLTEAGLIAELIVNLARNTSISFSHCRRECNFVAHQVARAAAENGDYFCVLGRSSSPLLEDEGVGGGACFPSWFVSVLANSCFGG
Homology
BLAST of Spg017085 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 73.6 bits (179), Expect = 1.9e-09
Identity = 44/123 (35.77%), Postives = 69/123 (56.10%), Query Frame = 0

Query: 6   EISTYRLGWVIRDSSGSLIGVGCKRVQRKWSITALEGEAMREGLEAYLKSYSSSNPRPPL 65
           + +T  +GW++RD  G +I   C+ ++ + +IT LE  A+ EGL A  + +       P+
Sbjct: 98  DTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIRQEHCR-----PI 157

Query: 66  AIESDSISLIKLISREDEDLTEAGLIAELIVNLARNTSI-SFSHCRRECNFVAHQVARAA 125
            +ESDS+  I L+ R+ +D TE   + E I  + ++  I S  H  RE N VAH +AR A
Sbjct: 158 HLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVAHGLARRA 215

Query: 126 AEN 128
            EN
Sbjct: 218 MEN 215

BLAST of Spg017085 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 71.6 bits (174), Expect = 7.1e-09
Identity = 44/126 (34.92%), Postives = 69/126 (54.76%), Query Frame = 0

Query: 6   EISTYRLGWVIRDSSGSLIGVGCKRVQRKWSITALEGEAMREGLEAYLKSYSSSNPRP-- 65
           + +T  +GW++RD  G +I   C+ ++ + +IT LE  A+ EGL A  + +     +   
Sbjct: 98  DTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEHCRPIQQEHC 157

Query: 66  -PLAIESDSISLIKLISREDEDLTEAGLIAELIVNLARNTSI-SFSHCRRECNFVAHQVA 125
            P+ +ESDS+  I L+ R+ +D TE   + E I  +  +  I S  H  RE N VAH +A
Sbjct: 158 RPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISREANKVAHDLA 217

Query: 126 RAAAEN 128
           R A EN
Sbjct: 218 RRAMEN 223

BLAST of Spg017085 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 68.6 bits (166), Expect = 6.0e-08
Identity = 52/160 (32.50%), Postives = 86/160 (53.75%), Query Frame = 0

Query: 8   STYR--LGWVIRDSSGSLIGVGCKRVQRKWSITALEGEAMREGLEAYLKSYSSSNPRPPL 67
           ST+R  +GW+IR   G ++  G + V+   ++  LE  A+ EG    L++ ++     PL
Sbjct: 90  STHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEG----LRNLTNLGVLRPL 149

Query: 68  AIESDSISLIKLISREDEDLTEAGLIAELIVNLARNTSI-SFSHCRRECNFVAHQVARAA 127
            IE+DS  +  L++R+ EDLT+ G + E I+NL  +  I +F+   RE N  AH +A+ A
Sbjct: 150 HIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLAQRA 209

Query: 128 AENGDYFCVLGRSSSPLLEDEGVGGGACFPSWFVSVLANS 165
           +        + R S   ++        CFP+W +S+LA S
Sbjct: 210 S--------VLRESMIWVD--------CFPNW-LSILAKS 228

BLAST of Spg017085 vs. NCBI nr
Match: XP_015381032.1 (uncharacterized protein LOC107174551 [Citrus sinensis])

HSP 1 Score: 65.1 bits (157), Expect = 6.7e-07
Identity = 39/116 (33.62%), Postives = 61/116 (52.59%), Query Frame = 0

Query: 12  LGWVIRDSSGSLIGVGCKRVQRKWSITALEGEAMREGLEAYLKSYSSSNPRPPLAIESDS 71
           LG V+RDS G+      K ++   S+   E  AM  GL+   K+  +         ESDS
Sbjct: 141 LGVVVRDSDGNCRAAAIKSLRLPGSVAMAEATAMEWGLQVAEKANITFR-----IFESDS 200

Query: 72  ISLIKLISREDEDLTEAG-LIAELIVNLARNTSISFSHCRRECNFVAHQVARAAAE 127
           + +I LI+++   LTE G LI+++  NL    +    HC R+CN+ AH +A+ A +
Sbjct: 201 LEVIDLINKKSSSLTEIGWLISDIQENLQNFQNFKAQHCPRDCNYAAHSLAKLALQ 251

BLAST of Spg017085 vs. NCBI nr
Match: XP_024041966.1 (uncharacterized protein LOC112099096 [Citrus clementina])

HSP 1 Score: 65.1 bits (157), Expect = 6.7e-07
Identity = 39/116 (33.62%), Postives = 61/116 (52.59%), Query Frame = 0

Query: 12  LGWVIRDSSGSLIGVGCKRVQRKWSITALEGEAMREGLEAYLKSYSSSNPRPPLAIESDS 71
           LG V+RDS G+      K ++   S+   E  AM  GL+   K+  +         ESDS
Sbjct: 128 LGVVVRDSDGNCRAAAIKSLRLPGSVAMAEATAMEWGLQVAEKANITFR-----IFESDS 187

Query: 72  ISLIKLISREDEDLTEAG-LIAELIVNLARNTSISFSHCRRECNFVAHQVARAAAE 127
           + +I LI+++   LTE G LI+++  NL    +    HC R+CN+ AH +A+ A +
Sbjct: 188 LEVIDLINKKSSSLTEIGWLISDIQENLQNFQNFKAQHCPRDCNYAAHSLAKLALQ 238

BLAST of Spg017085 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 9.1e-10
Identity = 44/123 (35.77%), Postives = 69/123 (56.10%), Query Frame = 0

Query: 6   EISTYRLGWVIRDSSGSLIGVGCKRVQRKWSITALEGEAMREGLEAYLKSYSSSNPRPPL 65
           + +T  +GW++RD  G +I   C+ ++ + +IT LE  A+ EGL A  + +       P+
Sbjct: 98  DTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIRQEHCR-----PI 157

Query: 66  AIESDSISLIKLISREDEDLTEAGLIAELIVNLARNTSI-SFSHCRRECNFVAHQVARAA 125
            +ESDS+  I L+ R+ +D TE   + E I  + ++  I S  H  RE N VAH +AR A
Sbjct: 158 HLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVAHGLARRA 215

Query: 126 AEN 128
            EN
Sbjct: 218 MEN 215

BLAST of Spg017085 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 3.5e-09
Identity = 44/126 (34.92%), Postives = 69/126 (54.76%), Query Frame = 0

Query: 6   EISTYRLGWVIRDSSGSLIGVGCKRVQRKWSITALEGEAMREGLEAYLKSYSSSNPRP-- 65
           + +T  +GW++RD  G +I   C+ ++ + +IT LE  A+ EGL A  + +     +   
Sbjct: 98  DTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEHCRPIQQEHC 157

Query: 66  -PLAIESDSISLIKLISREDEDLTEAGLIAELIVNLARNTSI-SFSHCRRECNFVAHQVA 125
            P+ +ESDS+  I L+ R+ +D TE   + E I  +  +  I S  H  RE N VAH +A
Sbjct: 158 RPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISREANKVAHDLA 217

Query: 126 RAAAEN 128
           R A EN
Sbjct: 218 RRAMEN 223

BLAST of Spg017085 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 2.9e-08
Identity = 52/160 (32.50%), Postives = 86/160 (53.75%), Query Frame = 0

Query: 8   STYR--LGWVIRDSSGSLIGVGCKRVQRKWSITALEGEAMREGLEAYLKSYSSSNPRPPL 67
           ST+R  +GW+IR   G ++  G + V+   ++  LE  A+ EG    L++ ++     PL
Sbjct: 90  STHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEG----LRNLTNLGVLRPL 149

Query: 68  AIESDSISLIKLISREDEDLTEAGLIAELIVNLARNTSI-SFSHCRRECNFVAHQVARAA 127
            IE+DS  +  L++R+ EDLT+ G + E I+NL  +  I +F+   RE N  AH +A+ A
Sbjct: 150 HIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLAQRA 209

Query: 128 AENGDYFCVLGRSSSPLLEDEGVGGGACFPSWFVSVLANS 165
           +        + R S   ++        CFP+W +S+LA S
Sbjct: 210 S--------VLRESMIWVD--------CFPNW-LSILAKS 228

BLAST of Spg017085 vs. ExPASy TrEMBL
Match: A0A6J5UV01 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS32434 PE=4 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 3.6e-06
Identity = 41/122 (33.61%), Postives = 60/122 (49.18%), Query Frame = 0

Query: 12   LGWVIRDSSGSLIGVGCKRVQRKWSITALEGEAMREGLEAYLKSYSSSNPRPPLAIESDS 71
            +GWV+RDS G ++  GCK   R  S  A+E +A+RE L A ++S  S      L +ESDS
Sbjct: 1084 VGWVVRDSYGWMVQAGCKGDMRGGSALAMEAKAIREALLACVQSGLSK-----LEVESDS 1143

Query: 72   ISLIKLISREDEDLTEAGLIAELIVNLARNTSISFSHC-----RRECNFVAHQVARAAAE 129
            + LI+++  E        +  E IV   +     F HC      R CN  AH++    + 
Sbjct: 1144 LQLIRMLGGE----WNIDIAVETIVFDIKQLVCQFQHCVFLYTPRNCNKAAHKITAFVSR 1196

BLAST of Spg017085 vs. ExPASy TrEMBL
Match: A0A6J5Y0D5 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS43248 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 4.7e-06
Identity = 38/111 (34.23%), Postives = 61/111 (54.95%), Query Frame = 0

Query: 13   GWVIRDSSGSLIGVGCKRVQRKWSITALEGEAMREGLEAYLKSYSSSNPRPPLAIESDSI 72
            G ++RDS G+L+G    R   + S+ A E  A++ G+     S++      PL IESDS+
Sbjct: 1019 GAIVRDSHGNLVGALAMRAPSRISVLATELYALKVGI-----SFALDVSPLPLEIESDSV 1078

Query: 73   SLIKLISREDEDL-TEAGLIAELIVNLARNTSISFSHCRRECNFVAHQVAR 123
              + +++ E+E L TE GL+  +   L R+ S    H  R+ N  AH++AR
Sbjct: 1079 QAVSMVNSEEECLTTEGGLVDGVRHLLVRSASTVVRHVPRQANKAAHRIAR 1124

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143535.11.9e-0935.77uncharacterized protein LOC111013412 [Momordica charantia][more]
XP_022156777.17.1e-0934.92uncharacterized protein LOC111023608 [Momordica charantia][more]
XP_022155262.16.0e-0832.50uncharacterized protein LOC111022403 [Momordica charantia][more]
XP_015381032.16.7e-0733.62uncharacterized protein LOC107174551 [Citrus sinensis][more]
XP_024041966.16.7e-0733.62uncharacterized protein LOC112099096 [Citrus clementina][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1CP269.1e-1035.77uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A6J1DSV13.5e-0934.92uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1DNV92.9e-0832.50uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J5UV013.6e-0633.61Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=... [more]
A0A6J5Y0D54.7e-0634.23Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS43248 PE=... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 4..133
e-value: 2.0E-8
score: 36.1
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 11..124
e-value: 1.5E-16
score: 60.3
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 7..126
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 7..126
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 13..122
e-value: 3.39381E-16
score: 68.4948
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 11..127

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg017085.1Spg017085.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity