Spg017003 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg017003
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionMechanosensitive ion channel protein
Locationscaffold9: 44447168 .. 44450414 (-)
RNA-Seq ExpressionSpg017003
SyntenySpg017003
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTGAACGGTAATAAGGAAGGTGAAAATGGAGGCCAAGTAGTGGTCGAAATGAGCAGCTCTGTATCTTCCAAGGAAAACAGAGCCGATTCGCAAATCCCCAGAACCCTCACGGCCAGGAAATCTCTCAAAAGACCAATCCTCCCGAAACCCAAATCAAGATTTGGGGAACAGTCGTATCGCATCGATTCAGACATGTTGGGAGAGAATAATCATGGATTCAGCTCTGCTCTGAACACGCCCAAGGAAGCAGACGACGACGACGAGGATATCGTCAAGATAGAGGCGTTTGACAAAGAGAAGAGCAAGAAACAGAAGGTGAAGAAATTGATAAAGTGGGTTGGAGTTGTTTGCATCATCAGCTGCTTGGTAGCTAGCTTGACCGTCGACCGTTTGAAGAAACGGTTCCTCTGGGGTTTGGAGGTTTGGGAATGGTGCTTGCTGGCAACCGTGATCATCTGTGGAATGATATTCAGCAATTGGGTAATGGAGGTGATTGTGAATTTGTGTGGGAGGAACTATCTGCTGGAGAAGAAACTGCTTTATTTTGCTCATGGGTTGAAGAAGAGCGGCCGAGTGGCACTTTGGCTGACATTGGTTCTTGTGACATGGGCGTCGTTGCTGAATTGGAGGAGATCGCGGATGGCTGGGAAGATTTTGGATGGCGTTACATGGAGTCTGGTGTGCCTTGTGATAGGGGCAGTTCTGTGGTTGGTAAAAACATTGTCCATGAAAATAGTGATGCACAAGCTGCGAATGAACCGATATTTCGACAGAATTCAGGATTCCATTTTCCATTATTATGTTCTACGGACGCTGTCGAGGCCTCCATCGGTCGGGATGGGCGAGGGCGGCGGCGAGTGGAGCGGCGGTCGATTGTGTTTCAAGTGTAAAAAAACAGAGCAAGAGCATGGTGAAAGGGTGATGTGGATTGACATGGGGAAGGCTCAACGGGTGGAGCGACACAAAGTTTCAGCTTGGACGATGAAGAGGTTGGTGGAGGCGGTTTCAAGTTCCGAGATGTCCATCTCACGAATACTCGATGAAAGTTGCCGGAATTTTGCTCATGCTGACATCGCCGATGACATGAAACATGCCGCCGCTGTCGCTTCCAAGATCTTCAACAATATTGATCGTGATGGAAACAAGTGACCAACTTTCCTTTTTTGTTTCTCATGTTTTCTTGAATTTCTTCATTTCTTTTTTTTTTTTTTTTTTTGTTAGTTGCATGTCAAATCTTTCTGAGAAATTTTTAGGTGCTTCCGATGCAACGAGGTCCGTAATAAATGGTAAAAGTACTTCGATATTGTATCCAACGGTTATTAGGGGACAGACAAATATAAAGAGGGTCTCATTACACTCGGAACACCTAATAATTTTCCATACATATCACCGTTTAAATGTTGACAACTAGGAGAGATAAAAATGAATTTTTTAGTTGTCAATATTTTTGGAGATCTCGACATAGTAGCTAAATATTGTTCTAATTTTTTTTTTATCCTTTCCCTTTTGTTAGGATCATTGAGAGAGCCAATCTTCGGGAATTCTTGAGCGAAGAACAAATTGATCGTGTGTTACCATACTTTGGAGTAGCTGAGACCGTGCACATTGACATAAAAGCTCTAACAGATTTTGTGGTGAGAACTTGTATCTATTATTTATCCCTTAGTCGTATCTAGCAAAATATGATTTTACTTTCGTAATTTTTTCTTATATAAAAATATTATTTAATTTCTTAGGATAATAAACATCAAAATTTACTTAAACACACTAACTTCAGAAAAACAACCCAACACCTTAGGCCCCGTTTGATAACCATTTTGTTTTTGGTTTTTGGTTTTTGAAAATTATGCTTGTTTTCTCCTAATTTCTTTATCATGATTTTCATCTTTGTTAAAGACACATTGGAATTCTTAGTCAAATTCCAAAAACAAAAACAAGTTTTTGAAAACTACTTTTTTTTGTTTTCAAAACATGAGTTGGTTTTTGAAAATATAGAAAGAATGTAAGTACAAAACAAAGAAACTTATGGAAAAAAATAGTGTTTATAGGCTTAATTTTCAAAAACCAAAAACCAAAAACAAAATGGTTATCAAACGGAGCCTTAAAGACAGAGTTCCTTAATTCAGACTAAGCTTTCTGTAATATGTATCCCAAACACATATTATTTAGCTCAACAAGATATTTTAACCGCAAACTATTATAATCCACTCAGCGTTCCCAAACAGTCCAGTAGAGTTTTTGTGGTCAAGACGTCTAAGAATACTTGATTGCAGACGTTTTCTGTGCGGTCTGAAGCCGTCATGGTTATGGCCAAAAATGTAGCACTTGCCTTGAATGTAGCCAAAGCGTCTCGACGATGTTCTGAGGCATGTAGGTAACTTGGTTTTTTTTGCAGGTGAAGGCTTATAAAGAGAGGGAAACAATAGCATATGCGTTGACTGACACTAAAACAGCTGTGAAGCAACTGAATAATTTAGTAACAGCGCTTGTTATAATAGTAACAGCTATTATTTGGCTTCTGTTGATGGAAATTGCTACAACCCAACTACTCCTCTTCCTTCTATCTCAACTTGCTGTGGTAGTTTTCATGTTCGGAAATTCTTGCAAGACTACATTCGAAGCTATAATCTTTGTGTTTGTTATCCATCCGTTCGATGTCGGGGACCGTTGTGTTGTCGATGACGTCCCGGTAAGCTTCTTAACTATCTAGCATGAAACAAAATCAAATTCACTTGTCAAAGTTCTCTTGTTTCTTCTTCTATGTGTAACTTTAATTATATATATTTGTGGCAATCTGCAGCTGTTGGTAGAAGAAATGAACATCTTGACAACAGTTTTCTTGAAACTCAACAATGAGAAGATGTGGTATCCCAACTCAGTTTTGGCAACAAAGCCCATCACTAATTACTACAGAAGTCCACACATGAGCGACACCGTAGAATTCTCGATCGGTTTCACGACGCCATTGGAGAGGATCGGGGCCATGAAAGAGAGAATAAAGAGGTATTTGAAGGAAAATCCACAACACTGGCATCCAGTACACAGTGTGGTGGTGAAGGAGATTGAAGATGTGAATAAGATAAAGATGGCTCTTTATACAAACCACACCATGAATTTTCAAGATTGGCCTGAAAAGACCAAACGAAGAACCGAGCTCATGATGGAGCTGAAGAAAATTTTCGAAGAACTGAACATCAACTACACTCTCCTGCCTCAAACAGTTCATCTCAATTATCATTAA

mRNA sequence

ATGGATGTGAACGGTAATAAGGAAGGTGAAAATGGAGGCCAAGTAGTGGTCGAAATGAGCAGCTCTGTATCTTCCAAGGAAAACAGAGCCGATTCGCAAATCCCCAGAACCCTCACGGCCAGGAAATCTCTCAAAAGACCAATCCTCCCGAAACCCAAATCAAGATTTGGGGAACAGTCGTATCGCATCGATTCAGACATGTTGGGAGAGAATAATCATGGATTCAGCTCTGCTCTGAACACGCCCAAGGAAGCAGACGACGACGACGAGGATATCGTCAAGATAGAGGCGTTTGACAAAGAGAAGAGCAAGAAACAGAAGGTGAAGAAATTGATAAAGTGGGTTGGAGTTGTTTGCATCATCAGCTGCTTGGTAGCTAGCTTGACCGTCGACCGTTTGAAGAAACGGTTCCTCTGGGGTTTGGAGGTTTGGGAATGGTGCTTGCTGGCAACCGTGATCATCTGTGGAATGATATTCAGCAATTGGGTAATGGAGGTGATTGTGAATTTGTGTGGGAGGAACTATCTGCTGGAGAAGAAACTGCTTTATTTTGCTCATGGGTTGAAGAAGAGCGGCCGAGTGGCACTTTGGCTGACATTGGTTCTTGTGACATGGGCGTCGTTGCTGAATTGGAGGAGATCGCGGATGGCTGGGAAGATTTTGGATGGCGTTACATGGAGTCTGGTGTGCCTTGTGATAGGGGCAGTTCTGTGGTTGGTAAAAACATTGTCCATGAAAATAGTGATGCACAAGCTGCGAATGAACCGATATTTCGACAGAATTCAGGATTCCATTTTCCATTATTATGTTCTACGGACGCTGTCGAGGCCTCCATCGGTCGGGATGGGCGAGGGCGGCGGCGAGTGGAGCGGCGGTCGATTGTGTTTCAAGTGTAAAAAAACAGAGCAAGAGCATGGTGAAAGGGTGATGTGGATTGACATGGGGAAGGCTCAACGGGTGGAGCGACACAAAGTTTCAGCTTGGACGATGAAGAGGTTGGTGGAGGCGGTTTCAAGTTCCGAGATGTCCATCTCACGAATACTCGATGAAAGTTGCCGGAATTTTGCTCATGCTGACATCGCCGATGACATGAAACATGCCGCCGCTGTCGCTTCCAAGATCTTCAACAATATTGATCGTGATGGAAACAAGATCATTGAGAGAGCCAATCTTCGGGAATTCTTGAGCGAAGAACAAATTGATCGTGTGTTACCATACTTTGGAGTAGCTGAGACCGTGCACATTGACATAAAAGCTCTAACAGATTTTGTGACGTTTTCTGTGCGGTCTGAAGCCGTCATGGTGAAGGCTTATAAAGAGAGGGAAACAATAGCATATGCGTTGACTGACACTAAAACAGCTGTGAAGCAACTGAATAATTTAGTAACAGCGCTTGTTATAATAGTAACAGCTATTATTTGGCTTCTGTTGATGGAAATTGCTACAACCCAACTACTCCTCTTCCTTCTATCTCAACTTGCTGTGGTAGTTTTCATGTTCGGAAATTCTTGCAAGACTACATTCGAAGCTATAATCTTTGTGTTTGTTATCCATCCGTTCGATGTCGGGGACCGTTGTGTTGTCGATGACGTCCCGCTGTTGGTAGAAGAAATGAACATCTTGACAACAGTTTTCTTGAAACTCAACAATGAGAAGATGTGGTATCCCAACTCAGTTTTGGCAACAAAGCCCATCACTAATTACTACAGAAGTCCACACATGAGCGACACCGTAGAATTCTCGATCGGTTTCACGACGCCATTGGAGAGGATCGGGGCCATGAAAGAGAGAATAAAGAGGTATTTGAAGGAAAATCCACAACACTGGCATCCAGTACACAGTGTGGTGGTGAAGGAGATTGAAGATGTGAATAAGATAAAGATGGCTCTTTATACAAACCACACCATGAATTTTCAAGATTGGCCTGAAAAGACCAAACGAAGAACCGAGCTCATGATGGAGCTGAAGAAAATTTTCGAAGAACTGAACATCAACTACACTCTCCTGCCTCAAACAGTTCATCTCAATTATCATTAA

Coding sequence (CDS)

ATGGATGTGAACGGTAATAAGGAAGGTGAAAATGGAGGCCAAGTAGTGGTCGAAATGAGCAGCTCTGTATCTTCCAAGGAAAACAGAGCCGATTCGCAAATCCCCAGAACCCTCACGGCCAGGAAATCTCTCAAAAGACCAATCCTCCCGAAACCCAAATCAAGATTTGGGGAACAGTCGTATCGCATCGATTCAGACATGTTGGGAGAGAATAATCATGGATTCAGCTCTGCTCTGAACACGCCCAAGGAAGCAGACGACGACGACGAGGATATCGTCAAGATAGAGGCGTTTGACAAAGAGAAGAGCAAGAAACAGAAGGTGAAGAAATTGATAAAGTGGGTTGGAGTTGTTTGCATCATCAGCTGCTTGGTAGCTAGCTTGACCGTCGACCGTTTGAAGAAACGGTTCCTCTGGGGTTTGGAGGTTTGGGAATGGTGCTTGCTGGCAACCGTGATCATCTGTGGAATGATATTCAGCAATTGGGTAATGGAGGTGATTGTGAATTTGTGTGGGAGGAACTATCTGCTGGAGAAGAAACTGCTTTATTTTGCTCATGGGTTGAAGAAGAGCGGCCGAGTGGCACTTTGGCTGACATTGGTTCTTGTGACATGGGCGTCGTTGCTGAATTGGAGGAGATCGCGGATGGCTGGGAAGATTTTGGATGGCGTTACATGGAGTCTGGTGTGCCTTGTGATAGGGGCAGTTCTGTGGTTGGTAAAAACATTGTCCATGAAAATAGTGATGCACAAGCTGCGAATGAACCGATATTTCGACAGAATTCAGGATTCCATTTTCCATTATTATGTTCTACGGACGCTGTCGAGGCCTCCATCGGTCGGGATGGGCGAGGGCGGCGGCGAGTGGAGCGGCGGTCGATTGTGTTTCAAGTGTAAAAAAACAGAGCAAGAGCATGGTGAAAGGGTGATGTGGATTGACATGGGGAAGGCTCAACGGGTGGAGCGACACAAAGTTTCAGCTTGGACGATGAAGAGGTTGGTGGAGGCGGTTTCAAGTTCCGAGATGTCCATCTCACGAATACTCGATGAAAGTTGCCGGAATTTTGCTCATGCTGACATCGCCGATGACATGAAACATGCCGCCGCTGTCGCTTCCAAGATCTTCAACAATATTGATCGTGATGGAAACAAGATCATTGAGAGAGCCAATCTTCGGGAATTCTTGAGCGAAGAACAAATTGATCGTGTGTTACCATACTTTGGAGTAGCTGAGACCGTGCACATTGACATAAAAGCTCTAACAGATTTTGTGACGTTTTCTGTGCGGTCTGAAGCCGTCATGGTGAAGGCTTATAAAGAGAGGGAAACAATAGCATATGCGTTGACTGACACTAAAACAGCTGTGAAGCAACTGAATAATTTAGTAACAGCGCTTGTTATAATAGTAACAGCTATTATTTGGCTTCTGTTGATGGAAATTGCTACAACCCAACTACTCCTCTTCCTTCTATCTCAACTTGCTGTGGTAGTTTTCATGTTCGGAAATTCTTGCAAGACTACATTCGAAGCTATAATCTTTGTGTTTGTTATCCATCCGTTCGATGTCGGGGACCGTTGTGTTGTCGATGACGTCCCGCTGTTGGTAGAAGAAATGAACATCTTGACAACAGTTTTCTTGAAACTCAACAATGAGAAGATGTGGTATCCCAACTCAGTTTTGGCAACAAAGCCCATCACTAATTACTACAGAAGTCCACACATGAGCGACACCGTAGAATTCTCGATCGGTTTCACGACGCCATTGGAGAGGATCGGGGCCATGAAAGAGAGAATAAAGAGGTATTTGAAGGAAAATCCACAACACTGGCATCCAGTACACAGTGTGGTGGTGAAGGAGATTGAAGATGTGAATAAGATAAAGATGGCTCTTTATACAAACCACACCATGAATTTTCAAGATTGGCCTGAAAAGACCAAACGAAGAACCGAGCTCATGATGGAGCTGAAGAAAATTTTCGAAGAACTGAACATCAACTACACTCTCCTGCCTCAAACAGTTCATCTCAATTATCATTAA

Protein sequence

MDVNGNKEGENGGQVVVEMSSSVSSKENRADSQIPRTLTARKSLKRPILPKPKSRFGEQSYRIDSDMLGENNHGFSSALNTPKEADDDDEDIVKIEAFDKEKSKKQKVKKLIKWVGVVCIISCLVASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYLLEKKLLYFAHGLKKSGRVALWLTLVLVTWASLLNWRRSRMAGKILDGVTWSLVCLVIGAVLWLVKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEGGGEWSGGRLCFKCKKTEQEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMSISRILDESCRNFAHADIADDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRVLPYFGVAETVHIDIKALTDFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLVTALVIIVTAIIWLLLMEIATTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDVGDRCVVDDVPLLVEEMNILTTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSIGFTTPLERIGAMKERIKRYLKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWPEKTKRRTELMMELKKIFEELNINYTLLPQTVHLNYH
Homology
BLAST of Spg017003 vs. NCBI nr
Match: XP_022138170.1 (mechanosensitive ion channel protein 10 [Momordica charantia] >XP_022138178.1 mechanosensitive ion channel protein 10 [Momordica charantia] >XP_022138186.1 mechanosensitive ion channel protein 10 [Momordica charantia] >XP_022138194.1 mechanosensitive ion channel protein 10 [Momordica charantia])

HSP 1 Score: 808.1 bits (2086), Expect = 5.6e-230
Identity = 468/750 (62.40%), Postives = 552/750 (73.60%), Query Frame = 0

Query: 1   MDVNGN------------KEGENGGQVVVEMSSSVSSKENR-------------ADSQ-- 60
           MDVNGN            KE ENGGQVVVE+S  V SKE R              DSQ  
Sbjct: 1   MDVNGNKPLKAVRRSSSQKESENGGQVVVEISGVVPSKETRDENGCSVPSQNRVVDSQNK 60

Query: 61  ---------------IPR---TLTARKSLKRPILPKPKSRFGEQS-YRIDSDMLGEN--- 120
                          IP    TL  RKSLKR I  KPKSRFGEQ  Y IDSDM  E+   
Sbjct: 61  GPTDSSIGSANKPPKIPNSSGTLMPRKSLKRSIQSKPKSRFGEQQPYYIDSDMFEEDRLS 120

Query: 121 -------------NHGFSSALNTPK-EADDDDEDIV-KIEAFDKEKSKKQKVKKLIKWVG 180
                        + G  SAL TP+ +A+++DED + K E   KEK KK K+K L+KWVG
Sbjct: 121 LREQIGATSSRRASPGHKSALTTPRAQAEEEDEDAIWKTEQLSKEKHKKLKMKTLVKWVG 180

Query: 181 VVCIISCLVASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYL 240
           V CII CLVASLTV+RL+  FLWGLE+W+WCLLATVI+CGMIF+ WVM VIV L  RN+L
Sbjct: 181 VFCIIGCLVASLTVNRLRNCFLWGLEIWKWCLLATVILCGMIFTKWVMNVIVFLIERNFL 240

Query: 241 LEKKLLYFAHGLKKSGRVALWLTLVLVTWASLL---NWR--RSRMAGKILDGVTWSLVCL 300
           L+KK+LYF HGLKK  +V LWLTLVL TWASL    N R  RS+ AGKILD  TW+LV L
Sbjct: 241 LKKKVLYFVHGLKKIVQVTLWLTLVLATWASLFDRSNHRVLRSKTAGKILDAGTWTLVSL 300

Query: 301 VIGAVLWLVKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGE--GGGEW 360
           +IGA LWLVKTL +KI+  K  MNR+FDRIQ+SIFH++VL+TLSRPP +G+ E     + 
Sbjct: 301 LIGAFLWLVKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLSRPPLMGVAECAASAKS 360

Query: 361 SGGRLCFKCKKTEQEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMSISRILD 420
           S GRL  K KK++ +       IDMGK  +++R KVSAWTMK LV+AV+SSE+SIS++LD
Sbjct: 361 SSGRLSLKGKKSDHK-----KVIDMGKIHQLKREKVSAWTMKVLVDAVTSSELSISQLLD 420

Query: 421 ESCRN----FAHADIADDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRVLP 480
           ES +N     A +DI D+M+ A A A +IFNN+   GNK IE  +L +F+ +E++D VLP
Sbjct: 421 ESYQNGGAEVADSDITDEMEVARAAAYRIFNNVALPGNKFIEEEDLLKFMIKEEVDLVLP 480

Query: 481 YFGVAETVHIDIKALTDFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLVTAL 540
            F VA+T  ID KALT++V          VK Y+ R+T+A+AL DTKTAVKQLNNLVTAL
Sbjct: 481 LFEVADTRQIDRKALTNWV----------VKVYQGRKTLAHALKDTKTAVKQLNNLVTAL 540

Query: 541 VIIVTAIIWLLLMEIATTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDVGDR 600
           +IIVTA++WLLLMEIATT++L+FLLSQLAV  FMFGN+CKTTFEA+IFVFV+HPFDVGDR
Sbjct: 541 LIIVTAVVWLLLMEIATTKVLVFLLSQLAVAAFMFGNTCKTTFEALIFVFVMHPFDVGDR 600

Query: 601 CVVDDVPLLVEEMNILTTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSIGFT 660
           CVVD VPLLVEEMNILTTVFLKL+NEK++YPNSVL+TKPITNYYRSP MSDTVEFSI FT
Sbjct: 601 CVVDGVPLLVEEMNILTTVFLKLSNEKVYYPNSVLSTKPITNYYRSPDMSDTVEFSISFT 660

Query: 661 TPLERIGAMKERIKRYLKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWPEKT 676
           TPLERIGAMKERIKRYL++N QHWHP H VVVKEIEDVNKIK+ALY NHT+NFQ+W EK 
Sbjct: 661 TPLERIGAMKERIKRYLEKNSQHWHPNHDVVVKEIEDVNKIKIALYVNHTLNFQEWAEKN 720

BLAST of Spg017003 vs. NCBI nr
Match: XP_023524086.1 (mechanosensitive ion channel protein 10-like [Cucurbita pepo subsp. pepo] >XP_023524087.1 mechanosensitive ion channel protein 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 800.4 bits (2066), Expect = 1.2e-227
Identity = 457/736 (62.09%), Postives = 539/736 (73.23%), Query Frame = 0

Query: 1   MDVNGN------------KEGENGGQVVVEMSSSVSS----KENRADSQ----------- 60
           MDVNGN            KE ENG QVVVE+S   +     K+NR DSQ           
Sbjct: 1   MDVNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY 60

Query: 61  ---------------IP---RTLTARKSLKRPILPKPKSRFGEQSYRIDSDMLGEN---- 120
                          IP    TLT RKSLKR IL KPKSRFGEQS  IDSD   EN    
Sbjct: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSFIDSDTFEENRLSL 120

Query: 121 ------NHGFSSALNTPKE-ADDDDEDIVKIEAFDKEKSKKQKVKKLIKWVGVVCIISCL 180
                 N    S LNTPKE  ++ D+DI KIE F KEK KK KV  LIKWVG  CII CL
Sbjct: 121 RDQISANSSRRSTLNTPKEHHEEGDDDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180

Query: 181 VASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYLLEKKLLYF 240
           VASLT+ RLKK FLWG+E+W+WCLLATVI+CGMIF++ VM VIV L  RN+LL+KK+ YF
Sbjct: 181 VASLTIKRLKKYFLWGIEIWKWCLLATVILCGMIFTHRVMNVIVFLIERNFLLQKKVFYF 240

Query: 241 AHGLKKSGRVALWLTLVLVTWASLLNWR-----RSRMAGKILDGVTWSLVCLVIGAVLWL 300
            HGLKKS +V LWLTLVLVTW SL +       RS+++GKILD +TW+L+ L+IGA LWL
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWVSLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300

Query: 301 VKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEGGGEWSGGRLCFKCK 360
           +KTL +KI+  K  MNR+FDRIQ+SIFH++VL+TL  P  +GM E   E + GRL FK K
Sbjct: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360

Query: 361 KTEQEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMSISRILDESCRNFAHAD 420
           K++ +       ID+GK  +++R KVSAWTMK LV+AV+SSEMSIS++LDES +  A  +
Sbjct: 361 KSDHK-----KVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQ-VADGE 420

Query: 421 IADDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRVLPYFGVAETVHIDIKA 480
           I D+ + A   A KIFNNI   GN  IE  +LR  + +E++D VLP F V ET  ID K+
Sbjct: 421 ITDETEVAGYAAFKIFNNIALPGNDFIEEEDLRRVMIKEEVDLVLPLFEVDETRRIDWKS 480

Query: 481 LTDFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLVTALVIIVTAIIWLLLME 540
           LT++V          +K YKER+T+A+AL DTKTAVKQLNNLVT L+IIVTAIIWLLLME
Sbjct: 481 LTNWV----------LKVYKERKTLAHALKDTKTAVKQLNNLVTVLLIIVTAIIWLLLME 540

Query: 541 IATTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDVGDRCVVDDVPLLVEEMN 600
           IATT++L+FLLSQLAV VFMFGN+CKT FEA++FVFV+HPFDVGDRC V+ VPL+VEEMN
Sbjct: 541 IATTKVLVFLLSQLAVAVFMFGNTCKTIFEALVFVFVMHPFDVGDRCAVEGVPLMVEEMN 600

Query: 601 ILTTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSIGFTTPLERIGAMKERIK 660
           ILTTVFLKLNNEK++YPNSVLATK ITNYYRSP MSDTVEFSIGF TPLERIG MK+RI+
Sbjct: 601 ILTTVFLKLNNEKVYYPNSVLATKSITNYYRSPDMSDTVEFSIGFATPLERIGLMKDRIR 660

Query: 661 RYLKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWPEKTKRRTELMMELKKIF 676
           RYL++NPQHWHP HSVVVKEIEDVNKIK ALY+NHTMNFQDW EK +RRTEL+MELK+IF
Sbjct: 661 RYLEKNPQHWHPNHSVVVKEIEDVNKIKFALYSNHTMNFQDWTEKNRRRTELVMELKRIF 720

BLAST of Spg017003 vs. NCBI nr
Match: XP_022940136.1 (mechanosensitive ion channel protein 10-like [Cucurbita moschata] >XP_022940137.1 mechanosensitive ion channel protein 10-like [Cucurbita moschata])

HSP 1 Score: 798.1 bits (2060), Expect = 5.8e-227
Identity = 456/736 (61.96%), Postives = 537/736 (72.96%), Query Frame = 0

Query: 1   MDVNGN------------KEGENGGQVVVEMSSSVSS----KENRADSQ----------- 60
           MD+NGN            KE ENG QVVVE+S   +     K+NR DSQ           
Sbjct: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPMDSSIGY 60

Query: 61  ---------------IP---RTLTARKSLKRPILPKPKSRFGEQSYRIDSDMLGENNHGF 120
                          IP    TLT RKSLKR IL KPKSRFGEQS  IDSD   EN   F
Sbjct: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF 120

Query: 121 ----------SSALNTPKE-ADDDDEDIVKIEAFDKEKSKKQKVKKLIKWVGVVCIISCL 180
                      S LNTPKE  ++ DEDI KIE F KEK KK KV  LIKWVG  CII CL
Sbjct: 121 RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180

Query: 181 VASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYLLEKKLLYF 240
           VASLT+ RLK  FLWG+E+W+WCLLATVI+CGMIF++ VM VIV L  RN+LL+KK+ YF
Sbjct: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHRVMNVIVFLIERNFLLQKKVFYF 240

Query: 241 AHGLKKSGRVALWLTLVLVTWASLLNWR-----RSRMAGKILDGVTWSLVCLVIGAVLWL 300
            HGLKKS +V LWLTLVLVTW SL +       RS+++GKILD +TW+L+ L+IGA LWL
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWVSLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300

Query: 301 VKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEGGGEWSGGRLCFKCK 360
           +KTL +KI+  K  MNR+FDRIQ+SIFH++VL+TL  P  +GM E   E + GRL FK K
Sbjct: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360

Query: 361 KTEQEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMSISRILDESCRNFAHAD 420
           K+  +       ID+GK  +++R KVSAWTMK LV+AV+SSEMSIS++LDES +  A  +
Sbjct: 361 KSNHK-----KVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQ-VADGE 420

Query: 421 IADDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRVLPYFGVAETVHIDIKA 480
           I D+ + A   A KIFNNI   GN  IE  +LR  + +E++D VLP F V ET  ID K+
Sbjct: 421 ITDETEVAGYAAFKIFNNIAVPGNDFIEEEDLRRVMIKEEVDLVLPLFEVDETRRIDWKS 480

Query: 481 LTDFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLVTALVIIVTAIIWLLLME 540
           LT++V          +K YKER+T+A+AL DTKTAVKQLNNLVT L+IIVTAIIWLLLME
Sbjct: 481 LTNWV----------LKVYKERKTLAHALKDTKTAVKQLNNLVTVLLIIVTAIIWLLLME 540

Query: 541 IATTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDVGDRCVVDDVPLLVEEMN 600
           IATT++L+FLLSQLAV VFMFGN+CKT FEA++FVFV+HPFDVGDRC V+ VPL+VEEMN
Sbjct: 541 IATTKVLVFLLSQLAVAVFMFGNTCKTIFEALVFVFVMHPFDVGDRCAVEGVPLMVEEMN 600

Query: 601 ILTTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSIGFTTPLERIGAMKERIK 660
           ILTTVFLKLNNEK++YPNSVLATK ITNYYRSP MSDTVEFSIGF TPLERIG MK+RI+
Sbjct: 601 ILTTVFLKLNNEKVYYPNSVLATKSITNYYRSPDMSDTVEFSIGFATPLERIGLMKDRIR 660

Query: 661 RYLKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWPEKTKRRTELMMELKKIF 676
           RYL++NPQHWHP HSVVVKEIEDVNKIK ALY+NHTMNFQDW EK +RRTEL+MELK+IF
Sbjct: 661 RYLEKNPQHWHPNHSVVVKEIEDVNKIKFALYSNHTMNFQDWTEKNRRRTELVMELKRIF 720

BLAST of Spg017003 vs. NCBI nr
Match: XP_022982339.1 (mechanosensitive ion channel protein 10-like [Cucurbita maxima] >XP_022982340.1 mechanosensitive ion channel protein 10-like [Cucurbita maxima])

HSP 1 Score: 792.3 bits (2045), Expect = 3.2e-225
Identity = 453/736 (61.55%), Postives = 537/736 (72.96%), Query Frame = 0

Query: 1   MDVNGN------------KEGENGGQVVVEMSSSVSS----KENRADSQ----------- 60
           MD+NGN            KE ENG QVVVE+S   +     K+NR DSQ           
Sbjct: 1   MDLNGNTPLKPIRRSSSQKESENGSQVVVEISRDENGHSVPKQNRVDSQTKEPMDSSIGY 60

Query: 61  ---------------IP---RTLTARKSLKRPILPKPKSRFGEQSYRIDSDMLGENNHGF 120
                          IP    TLT RKSLKR IL KPKSRFGEQS  IDSD   EN   F
Sbjct: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSVIDSDTFEENRLSF 120

Query: 121 ----------SSALNTPKE-ADDDDEDIVKIEAFDKEKSKKQKVKKLIKWVGVVCIISCL 180
                      S LN PKE  ++ D++  KIE F K K KK K   LIKWVG  CII CL
Sbjct: 121 RDQISANSSRRSTLNMPKEHHEEGDDEFFKIEKFKKVKHKKVKAITLIKWVGAFCIIGCL 180

Query: 181 VASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYLLEKKLLYF 240
           VASLT+ RLK  FLWG+E+W+WCLLATVI+CGMIF++ VM VIV L  RN+LL+KK+ YF
Sbjct: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHRVMNVIVFLIERNFLLQKKVFYF 240

Query: 241 AHGLKKSGRVALWLTLVLVTWASLLNWR-----RSRMAGKILDGVTWSLVCLVIGAVLWL 300
            HGLKKS +V LWLTLVLVTW SL +       RS+++GKILD +TW+L+ L+IGA LWL
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWVSLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300

Query: 301 VKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEGGGEWSGGRLCFKCK 360
           +KTL +KI+  K  MNR+FDRIQ+SIFH++VL+TL  P  +GM E   E + GRL FK K
Sbjct: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360

Query: 361 KTEQEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMSISRILDESCRNFAHAD 420
           K++ +       ID+GK  +++R KVSAWTMK LV+AV+SSEMSIS++LDES +  A  +
Sbjct: 361 KSDHK-----KVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQ-VADGE 420

Query: 421 IADDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRVLPYFGVAETVHIDIKA 480
           IAD+M+ A   A KIF+NI   GN  IE  +LR  + +E++D VLP F V ET  ID K+
Sbjct: 421 IADEMEVAGYAAYKIFDNIAVPGNDFIEEEDLRRVMIKEEVDLVLPLFEVDETRRIDWKS 480

Query: 481 LTDFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLVTALVIIVTAIIWLLLME 540
           LT++V          +K YKER+T+A+AL DTKTAVKQLNNLVTAL+IIVTAIIWLLLME
Sbjct: 481 LTNWV----------LKVYKERKTLAHALKDTKTAVKQLNNLVTALLIIVTAIIWLLLME 540

Query: 541 IATTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDVGDRCVVDDVPLLVEEMN 600
           IATT++L+FLLSQLAV VFMFGN+CKT FEA++FVFV+HPFDVGDRC V+ VPL+VEEMN
Sbjct: 541 IATTKVLVFLLSQLAVAVFMFGNTCKTIFEALVFVFVMHPFDVGDRCAVEGVPLMVEEMN 600

Query: 601 ILTTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSIGFTTPLERIGAMKERIK 660
           ILTTVFLKLNNEK++YPNSVLATK ITNYYRSP MSDTVEFSIGF TPLERIG MK+RIK
Sbjct: 601 ILTTVFLKLNNEKVYYPNSVLATKSITNYYRSPDMSDTVEFSIGFATPLERIGLMKDRIK 660

Query: 661 RYLKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWPEKTKRRTELMMELKKIF 676
           RYL++NPQHWHP HSVVVKEIEDVNKIK ALY+NHTMNFQDW EK +RRTEL+MELK+IF
Sbjct: 661 RYLEKNPQHWHPNHSVVVKEIEDVNKIKFALYSNHTMNFQDWTEKNRRRTELVMELKRIF 720

BLAST of Spg017003 vs. NCBI nr
Match: KAG6608342.1 (Mechanosensitive ion channel protein 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 783.5 bits (2022), Expect = 1.5e-222
Identity = 455/762 (59.71%), Postives = 538/762 (70.60%), Query Frame = 0

Query: 1   MDVNGN------------KEGENGGQVVVEMSSSVSS----KENRADSQ----------- 60
           MD+NGN            KE ENG QVVVE+S   +     K+NR DSQ           
Sbjct: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY 60

Query: 61  ---------------IP---RTLTARKSLKRPILPKPKSRFGEQSYRIDSDMLGENNHGF 120
                          IP    TLT RKSLKR IL KPKSRFGEQS  IDSD   EN   F
Sbjct: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF 120

Query: 121 ----------SSALNTPKE-ADDDDEDIVKIEAFDKEKSKKQKVKKLIKWVGVVCIISCL 180
                      S LNTPKE  ++ DEDI KIE F KEK KK KV  LIKWVG  CII CL
Sbjct: 121 RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180

Query: 181 VASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYLLEKKLLYF 240
           VASLT+ RLK  FLWG+E+W+WCLLATVI+CGMIF++ VM VIV L  RN+LL+KK+ YF
Sbjct: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHRVMNVIVFLIERNFLLQKKVFYF 240

Query: 241 AHGLKKSGRVALWLTLVLVTWASLLNWR-----RSRMAGKILDGVTWSLVCLVIGAVLWL 300
            HGLKKS +V LWLTLVLVTW +L +       RS+++GKILD +TW+L+ L+IGA LWL
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300

Query: 301 VKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEGGGEWSGGRLCFKCK 360
           +KTL +KI+  K  MNR+FDRIQ+SIFH++VL+TL  P  +GM E   E + GRL FK K
Sbjct: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360

Query: 361 KTEQEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMSISRILDESCRNFAHAD 420
           K++ +       ID+GK  +++R KVSAWTMK LV+AV+SSEMSIS++LDES +  A  +
Sbjct: 361 KSDHK-----KVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQ-VADGE 420

Query: 421 IADDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRVLPYFGVAETVHIDIKA 480
           I D+ + A   A KIFNNI   GN  IE  +LR  + +E++D VLP F V ET  ID K+
Sbjct: 421 ITDETEVAGYAAFKIFNNIAVPGNDFIEEEDLRRVMIKEEVDLVLPLFEVDETRRIDWKS 480

Query: 481 LTDFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLVTALVIIVTAIIWLLLME 540
           LT++V          +K YKER+T+A+AL DTKTAVKQLNNLVT L+IIVTAIIWLLLME
Sbjct: 481 LTNWV----------LKVYKERKTLAHALKDTKTAVKQLNNLVTVLLIIVTAIIWLLLME 540

Query: 541 IATTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDVGDRCVVDDVPLLVEEMN 600
           IATT++L+FLLSQLAV VFMFGN+CKT FEA++FVFV+HPFDVGDRC V+ VPL+VEEMN
Sbjct: 541 IATTKVLVFLLSQLAVAVFMFGNTCKTIFEALVFVFVMHPFDVGDRCAVEGVPLMVEEMN 600

Query: 601 ILTTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSIGFTTPLERIGAMKERIK 660
           ILTTVFLKLNNEK++YPNSVLATK ITNYYRSP MSDTVEFSIGF TPLERIG MK+RI+
Sbjct: 601 ILTTVFLKLNNEKVYYPNSVLATKSITNYYRSPDMSDTVEFSIGFATPLERIGLMKDRIR 660

Query: 661 --------------------------RYLKENPQHWHPVHSVVVKEIEDVNKIKMALYTN 676
                                     RYL++NPQHWHP HSVVVKEIEDVNKIK ALY+N
Sbjct: 661 RWVKVEQITKTLPFLEYNPYLTDGFCRYLEKNPQHWHPNHSVVVKEIEDVNKIKFALYSN 720

BLAST of Spg017003 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 1.3e-157
Identity = 334/693 (48.20%), Postives = 454/693 (65.51%), Query Frame = 0

Query: 18  EMSSSVSS--KENRADSQIPRTLTARKSLKRPILPKPKSRFGEQSYRIDSDMLGEN---- 77
           E+S  V S  K  RA +Q    LT RKS  R +  KPKSRF + S  +D+ +L E     
Sbjct: 50  EISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEEVREQ 109

Query: 78  -NHGFSSALNTPKEAD-------------------DDDEDIVKIEAFDKEKSKKQKVKKL 137
              GFS +  +P                       D+DE+I K    ++E   K     L
Sbjct: 110 LGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLAL 169

Query: 138 IKWVGVVCIISCLVASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLC 197
           I+    V I+S LVASLT++ LK    WGLEVW+WC+L  VI  GM+ +NW M +IV L 
Sbjct: 170 IESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLI 229

Query: 198 GRNYLLEKKLLYFAHGLKKSGRVALWLTLVLVTWASLLNW--RRSRMAGKILDGVTWSLV 257
             N+LL +K+LYF HGLKKS +V +WL L+LV W  L N   +RS  A K+L  +T +L+
Sbjct: 230 ETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLI 289

Query: 258 CLVIGAVLWLVKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEG-GGE 317
            ++ GA  WLVKTL +KI+     +N +FDRIQDS+FH YVL+TLS  P +   E  G E
Sbjct: 290 SILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGRE 349

Query: 318 WSGGRLCFKCKKTEQEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMS-ISRI 377
            S G L F     +    E+ + IDMGK  +++R KVSAWTM+ L+EAV +S +S IS  
Sbjct: 350 PSTGHLSFATVVKKGTVKEKKV-IDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDT 409

Query: 378 LDESC----RNFAHADIADDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRV 437
           LDE+     +  A  +I  +M+ A A A  +F N+ +     IE  +L  F+ +E++D V
Sbjct: 410 LDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLV 469

Query: 438 LPYF-GVAETVHIDIKALTDFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLV 497
            P F G AET  I  KA T++V          VK Y  R  +A++L DTKTAVKQLN LV
Sbjct: 470 FPLFDGAAETGRITRKAFTEWV----------VKVYTSRRALAHSLNDTKTAVKQLNKLV 529

Query: 498 TALVIIVTAIIWLLLMEIATTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDV 557
           TA++++VT +IWLLL+E+ATT++LLF  +QL  + F+ G++CK  FE+I+FVFV+HP+DV
Sbjct: 530 TAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDV 589

Query: 558 GDRCVVDDVPLLVEEMNILTTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSI 617
           GDRCVVD V +LVEEMN+LTTVFLKLNNEK++YPN+VLATKPI+NY+RSP+M +TVEFSI
Sbjct: 590 GDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSI 649

Query: 618 GFTTPLERIGAMKERIKRYLKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWP 676
            F+TP+ +I  +KERI  YL++NPQHW PVHSVVVKEIE++NK+KMALY++HT+ FQ+  
Sbjct: 650 SFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENR 709

BLAST of Spg017003 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 4.2e-143
Identity = 296/680 (43.53%), Postives = 433/680 (63.68%), Query Frame = 0

Query: 35  PRTLTARKSLKRPILPKPKSRFGE-QSYRIDS------------------------DMLG 94
           P  L  RKSL R I  KPKSRFGE QS+R DS                        D   
Sbjct: 75  PEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQFGAGSFARGSFDRAS 134

Query: 95  ENNHG----FSSALN-TPKEADDDDEDIVKIEAFDKEKSKKQKVKKLIKWVGVVCIISCL 154
            NN       S+AL+   +E  D++E+I K     + K    K    ++ V  + I+  L
Sbjct: 135 PNNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGMKPLAFLELVVFMAILGAL 194

Query: 155 VASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYLLEKKLLYF 214
           + SLT+D + K  +WGLE W+WC+L  V + GM+ +NW M  +V +  +NYLL KK+LYF
Sbjct: 195 IVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHFVVFIIEKNYLLRKKVLYF 254

Query: 215 AHGLKKSGRVALWLTLVLVTWASLLNW--RRSRMAGKILDGVTWSLVCLVIGAVLWLVKT 274
            HGLKK+ +V +W +LVL+ W  L +   +R+R   + LD +TW++V L++G++L+LVKT
Sbjct: 255 VHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKTKRFLDFITWTIVSLLVGSILFLVKT 314

Query: 275 LSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEGGGEW-SGGRLCFKCKKT 334
            ++K++  K  +  +F+RIQ+S+FH YVL+TLS PP +   E  G   S G L F   K 
Sbjct: 315 FALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEEAENVGRVPSTGHLSFTRTKD 374

Query: 335 EQEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMS-ISRILDE--SCRNFAHA 394
            +   ++V  IDMGK  R+++ KVSAWTM+ L+EAV +S +S IS  LDE  + +     
Sbjct: 375 GKVKDKKV--IDMGKVHRMKQEKVSAWTMRVLIEAVGTSGISTISSTLDEVNNKKERTDK 434

Query: 395 DIADDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRVLPYFGVAETVHIDIK 454
           +I ++M+ A A A  +FNN+ +  +  IE  +L  F+ +E++D VLP    A+T  I  K
Sbjct: 435 EITNEME-AVAAAYDVFNNVAKPNHNYIEEDDLLRFMIKEEVDLVLPLIEDADTGKITRK 494

Query: 455 ALTDFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLVTALVIIVTAIIWLLLM 514
             T++V          V  Y  R+TI ++L DTKTAVKQL+ L+T ++ ++T I+W++L+
Sbjct: 495 TFTEWV----------VNVYTSRKTIGHSLNDTKTAVKQLDKLITGILTVITFIVWMVLL 554

Query: 515 EIATTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDVGDRCVVDDVPLLVEEM 574
           +IA+T+LLL   SQ   + FM G++CK  FE+ +FVFV+HP+DVGDRCVVD V LLVEE+
Sbjct: 555 DIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCVVDGVMLLVEEI 614

Query: 575 NILTTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSIGFTTPLERIGAMKERI 634
           ++LTTVFLK++NEK++YPNSVL +KPI+N+YRSP M D V+F I F+TP E+IG +K +I
Sbjct: 615 DLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTPAEKIGCLKGKI 674

Query: 635 KRYLKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWPEKTKRRTELMMELKKI 679
             YL  N QHW+P   V+V+ IE++NK+ + +   HT+NFQ + EK+ RRT L++ +K+I
Sbjct: 675 GEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVEKSLRRTALIIAIKRI 734

BLAST of Spg017003 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 6.1e-110
Identity = 257/722 (35.60%), Postives = 398/722 (55.12%), Query Frame = 0

Query: 17  VEMSSSVSSKENRAD-SQIPRTLTARKSLKRPILPKPK--SRFGEQSYRIDSDML----- 76
           V+   S   +ENR D S++ +  + R +++   L K K  SR  +       DM+     
Sbjct: 148 VKDGDSSEDEENRVDGSEVVKCTSNRSTMRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTP 207

Query: 77  --GENNHGFSSALNTP--------KEADDDDEDIVKIEAFDKEKSKKQKVKKLIKWVGVV 136
             G  N GFS     P        K+ +++++   + +  +  + +K  V  +I+W+ ++
Sbjct: 208 RSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLI 267

Query: 137 CIISCLVASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYLLE 196
            II+ L+ SL +  L+ + LW L +W+W ++  V+ICG + S+W++++ V     N+L  
Sbjct: 268 LIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWR 327

Query: 197 KKLLYFAHGLKKSGRVALWLTLVLVTWASLLNWRRSR-MAGKILDGVTWSLVCLVIGAVL 256
           KK+LYF +G++K  +  LWL LVL+ W  L + +  R M   +L  VT  L+CL++  ++
Sbjct: 328 KKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVII 387

Query: 257 WLVKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTL----------------------- 316
           WL+KTL +K++     M+ YFDRIQ+S+F  YV+ TL                       
Sbjct: 388 WLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFE 447

Query: 317 --------------SRPPSVGMGEGGGEWSGGRL----CFKCKKTEQEHGERVMWIDMGK 376
                         S PP V +G G  + S  R+          +++E GE  + ID   
Sbjct: 448 IVGRKLSPLGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRID--H 507

Query: 377 AQRVERHKVSAWTMKRLVEAVSSSEMSI--SRILDESCRNFAHADIADDMKHAAAVASKI 436
            QR+    VSAW MK+L+  +    +S    +I D + +    A        A   A KI
Sbjct: 508 LQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKI 567

Query: 437 FNNIDRDGNKIIERANLREFLSEEQIDRVLPYF-GVAETVHIDIKALTDFVTFSVRSEAV 496
           F N+   G++ I   +   FLSE++ +R +  F G +E   I    L ++V         
Sbjct: 568 FQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWV--------- 627

Query: 497 MVKAYKERETIAYALTDTKTAVKQLNNLVTALVIIVTAIIWLLLMEIATTQLLLFLLSQL 556
            V A++ER  +A  L DTKTAV +L+ +V  LV IV  IIWLL++ IATT+ LL + SQL
Sbjct: 628 -VNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQL 687

Query: 557 AVVVFMFGNSCKTTFEAIIFVFVIHPFDVGDRCVVDDVPLLVEEMNILTTVFLKLNNEKM 616
            +VVF+FGNSCKT FEA+IFVFV+HPFDVGDRC +D V ++VEEMNILTTVFL+ +N+K+
Sbjct: 688 LLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKI 747

Query: 617 WYPNSVLATKPITNYYRSPHMSDTVEFSIGFTTPLERIGAMKERIKRYLKENPQHWHPVH 676
            YPNS+L TKPI NYYRSP M D +EF +   TP E+  A+++RI  Y+     HWHP  
Sbjct: 748 VYPNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSP 807

BLAST of Spg017003 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.3e-109
Identity = 259/713 (36.33%), Postives = 400/713 (56.10%), Query Frame = 0

Query: 4   NGNKEGENGGQVVVEMSSS----VSSKENRADSQIPRTLTARKSLKRPILPKPKSRFGEQ 63
           + ++EG N  +  V    S    +S  + ++  Q P T T       P + K + + G +
Sbjct: 166 SSDEEGRNRDEAEVLKCGSKKPMLSRNKTKSRLQDPPTPT------HPAIDKTEMKSGRR 225

Query: 64  SYRIDSDMLGENNHGFSSALNTPKEADDDDEDIVKIEAFDKEKSKKQKVKKLIKWVGVVC 123
           S    S  LG++    +   N  +E +++D   +  +  ++ K  K      ++W+ +V 
Sbjct: 226 SGIFKSGFLGKSPKAGTPGRNGFEEEEEED-PFLDEDLPEEFKRDKLSFWVFLEWISLVL 285

Query: 124 IISCLVASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYLLEK 183
           I++ LV SLT+  L+++  W L++W+W +   V+ICG + S+W++ +IV L  +N+   K
Sbjct: 286 IVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRK 345

Query: 184 KLLYFAHGLKKSGRVALWLTLVLVTWASLLNWRRSR-MAGKILDGVTWSLVCLVIGAVLW 243
           ++LYF +G++KS +  LWL LVL+ W  L + +  R      L  VT  LVCL++  ++W
Sbjct: 346 RVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVLVCLLVALIIW 405

Query: 244 LVKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEGGGE---------- 303
           LVKT+ +K++     M+ YFDRIQ+S+F  YV+ TLS PP + +     E          
Sbjct: 406 LVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKS 465

Query: 304 ---WSGGRLCFKCKKTEQ----------------EHGERVMWIDMGKAQRVERHKVSAWT 363
               +G +L    K T +                + GE    I + + +R+    VSAW 
Sbjct: 466 LEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWN 525

Query: 364 MKRLVEAVSSSEMSISRILDESCRNFAHADIADDMKH------AAAVASKIFNNIDRDGN 423
           MKRL+  +    +S    LD++ ++    D  +D  H      A   A KIF+N+   G+
Sbjct: 526 MKRLMNIILKGAIS---TLDQNMQDTTQED--EDATHIRSEYEAKCAARKIFHNVTEPGS 585

Query: 424 KIIERANLREFLSEEQIDRVLPYF-GVAETVHIDIKALTDFVTFSVRSEAVMVKAYKERE 483
           + I   +   FL EE+ +R +  F G +E+  I    L ++V          VKA++ER 
Sbjct: 586 RYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWV----------VKAFRERR 645

Query: 484 TIAYALTDTKTAVKQLNNLVTALVIIVTAIIWLLLMEIATTQLLLFLLSQLAVVVFMFGN 543
            +A  L DTKTAV +L+ ++  ++ I+  IIWLL++ IATT+ LL L SQL +V F+FGN
Sbjct: 646 ALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGN 705

Query: 544 SCKTTFEAIIFVFVIHPFDVGDRCVVDDVPLLVEEMNILTTVFLKLNNEKMWYPNSVLAT 603
           SCKT FEAIIF+FV+HPFDVGDRC +D V L+VEEMNILTTVFL+ +N+K+ YPNSVL T
Sbjct: 706 SCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGT 765

Query: 604 KPITNYYRSPHMSDTVEFSIGFTTPLERIGAMKERIKRYLKENPQHWHPVHSVVVKEIED 663
           KPI NYYRSP M D VEF +   TP E+I A+K+RI  Y+     +W+P   +V   ++D
Sbjct: 766 KPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDD 825

Query: 664 VNKIKMALYTNHTMNFQDWPEKTKRRTELMMELKKIFEELNINYTLLPQTVHL 676
           +N +K+A++  H MN QD  E+  RR  L+ E+ K   EL+I Y L P  +++
Sbjct: 826 LNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINV 856

BLAST of Spg017003 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 7.4e-108
Identity = 248/714 (34.73%), Postives = 400/714 (56.02%), Query Frame = 0

Query: 2   DVNGNKEGE----NGGQVVVEMSSSVSSKENRADSQIPRTLTARKSLKRPILPK-PKSRF 61
           + N N +GE    +G    ++ SSS   K           +  R  L  P  P+ P    
Sbjct: 142 NTNNNDDGEVVKCSGNNAPIQRSSSTLLK-----------MRTRSRLSDPPTPQLPPQTA 201

Query: 62  GEQSYRIDSDMLGENNHGFSSALNTPKEADDDDEDIVKIEAFDKEKSKKQKVKKLIKWVG 121
             +S RI     G+   GF       +  +++D+     +  ++ +  K  +  +++W+ 
Sbjct: 202 DMKSGRIPKS--GQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLS 261

Query: 122 VVCIISCLVASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYL 181
           ++ II+  V +L +  L+K+ LW L++W+W  +  V+ICG + S+W+++++V    RN+L
Sbjct: 262 LILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFL 321

Query: 182 LEKKLLYFAHGLKKSGRVALWLTLVLVTWASLLNWRRSRMAG-KILDGVTWSLVCLVIGA 241
           L K++LYF +G++K+ +  LWL LVL+ W  L + + ++ A  K L  VT   VCL++G 
Sbjct: 322 LRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGF 381

Query: 242 VLWLVKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEGGGEWSGGRLC 301
           +LWLVKTL +K++     M+ YFDRIQ+S+F  YV+ TLS PP + + +   E    R+ 
Sbjct: 382 LLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQK--NEEEEERIS 441

Query: 302 FKCKKTEQEHGERVM-------------------------------WIDMGKAQRVERHK 361
            + KK +   G  +                                 I +    ++    
Sbjct: 442 VEVKKFQNPGGVEIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKN 501

Query: 362 VSAWTMKRLVEAVSSSEMSI--SRILDESCRNFAHADIADDMKHAAAVASKIFNNIDRDG 421
           VSAW MKRL+  + +  ++    ++ D S  +     I  + + A   A KIF+N+ + G
Sbjct: 502 VSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFE-AKLAARKIFHNVAKPG 561

Query: 422 NKIIERANLREFLSEEQIDRVLPYF-GVAETVHIDIKALTDFVTFSVRSEAVMVKAYKER 481
           +K I   ++  FL +++  + L  F G +ET  I   +L ++V          V A++ER
Sbjct: 562 SKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWV----------VNAFRER 621

Query: 482 ETIAYALTDTKTAVKQLNNLVTALVIIVTAIIWLLLMEIATTQLLLFLLSQLAVVVFMFG 541
             +A  L DTKTAV +L+ +V  +V I+  +IWL+++ I +T+ L+ + SQ+ VV F+FG
Sbjct: 622 RALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFG 681

Query: 542 NSCKTTFEAIIFVFVIHPFDVGDRCVVDDVPLLVEEMNILTTVFLKLNNEKMWYPNSVLA 601
           N CK  FE+II++FVIHPFDVGDRC +D V ++VEEMNILTTVFL+ +N+K+ YPNS+L 
Sbjct: 682 NMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLW 741

Query: 602 TKPITNYYRSPHMSDTVEFSIGFTTPLERIGAMKERIKRYLKENPQHWHPVHSVVVKEIE 661
           TK I NYYRSP M D +EFSI  TTP E+I  +K+RI  Y++    HW+P   +V K++E
Sbjct: 742 TKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDME 801

Query: 662 DVNKIKMALYTNHTMNFQDWPEKTKRRTELMMELKKIFEELNINYTLLPQTVHL 676
            +N +++A++  H MN QD  EK  RR++L+ E+ KI  EL+I Y L P  +++
Sbjct: 802 SLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINV 829

BLAST of Spg017003 vs. ExPASy TrEMBL
Match: A0A6J1C8Z8 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111009404 PE=3 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 2.7e-230
Identity = 468/750 (62.40%), Postives = 552/750 (73.60%), Query Frame = 0

Query: 1   MDVNGN------------KEGENGGQVVVEMSSSVSSKENR-------------ADSQ-- 60
           MDVNGN            KE ENGGQVVVE+S  V SKE R              DSQ  
Sbjct: 1   MDVNGNKPLKAVRRSSSQKESENGGQVVVEISGVVPSKETRDENGCSVPSQNRVVDSQNK 60

Query: 61  ---------------IPR---TLTARKSLKRPILPKPKSRFGEQS-YRIDSDMLGEN--- 120
                          IP    TL  RKSLKR I  KPKSRFGEQ  Y IDSDM  E+   
Sbjct: 61  GPTDSSIGSANKPPKIPNSSGTLMPRKSLKRSIQSKPKSRFGEQQPYYIDSDMFEEDRLS 120

Query: 121 -------------NHGFSSALNTPK-EADDDDEDIV-KIEAFDKEKSKKQKVKKLIKWVG 180
                        + G  SAL TP+ +A+++DED + K E   KEK KK K+K L+KWVG
Sbjct: 121 LREQIGATSSRRASPGHKSALTTPRAQAEEEDEDAIWKTEQLSKEKHKKLKMKTLVKWVG 180

Query: 181 VVCIISCLVASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYL 240
           V CII CLVASLTV+RL+  FLWGLE+W+WCLLATVI+CGMIF+ WVM VIV L  RN+L
Sbjct: 181 VFCIIGCLVASLTVNRLRNCFLWGLEIWKWCLLATVILCGMIFTKWVMNVIVFLIERNFL 240

Query: 241 LEKKLLYFAHGLKKSGRVALWLTLVLVTWASLL---NWR--RSRMAGKILDGVTWSLVCL 300
           L+KK+LYF HGLKK  +V LWLTLVL TWASL    N R  RS+ AGKILD  TW+LV L
Sbjct: 241 LKKKVLYFVHGLKKIVQVTLWLTLVLATWASLFDRSNHRVLRSKTAGKILDAGTWTLVSL 300

Query: 301 VIGAVLWLVKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGE--GGGEW 360
           +IGA LWLVKTL +KI+  K  MNR+FDRIQ+SIFH++VL+TLSRPP +G+ E     + 
Sbjct: 301 LIGAFLWLVKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLSRPPLMGVAECAASAKS 360

Query: 361 SGGRLCFKCKKTEQEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMSISRILD 420
           S GRL  K KK++ +       IDMGK  +++R KVSAWTMK LV+AV+SSE+SIS++LD
Sbjct: 361 SSGRLSLKGKKSDHK-----KVIDMGKIHQLKREKVSAWTMKVLVDAVTSSELSISQLLD 420

Query: 421 ESCRN----FAHADIADDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRVLP 480
           ES +N     A +DI D+M+ A A A +IFNN+   GNK IE  +L +F+ +E++D VLP
Sbjct: 421 ESYQNGGAEVADSDITDEMEVARAAAYRIFNNVALPGNKFIEEEDLLKFMIKEEVDLVLP 480

Query: 481 YFGVAETVHIDIKALTDFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLVTAL 540
            F VA+T  ID KALT++V          VK Y+ R+T+A+AL DTKTAVKQLNNLVTAL
Sbjct: 481 LFEVADTRQIDRKALTNWV----------VKVYQGRKTLAHALKDTKTAVKQLNNLVTAL 540

Query: 541 VIIVTAIIWLLLMEIATTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDVGDR 600
           +IIVTA++WLLLMEIATT++L+FLLSQLAV  FMFGN+CKTTFEA+IFVFV+HPFDVGDR
Sbjct: 541 LIIVTAVVWLLLMEIATTKVLVFLLSQLAVAAFMFGNTCKTTFEALIFVFVMHPFDVGDR 600

Query: 601 CVVDDVPLLVEEMNILTTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSIGFT 660
           CVVD VPLLVEEMNILTTVFLKL+NEK++YPNSVL+TKPITNYYRSP MSDTVEFSI FT
Sbjct: 601 CVVDGVPLLVEEMNILTTVFLKLSNEKVYYPNSVLSTKPITNYYRSPDMSDTVEFSISFT 660

Query: 661 TPLERIGAMKERIKRYLKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWPEKT 676
           TPLERIGAMKERIKRYL++N QHWHP H VVVKEIEDVNKIK+ALY NHT+NFQ+W EK 
Sbjct: 661 TPLERIGAMKERIKRYLEKNSQHWHPNHDVVVKEIEDVNKIKIALYVNHTLNFQEWAEKN 720

BLAST of Spg017003 vs. ExPASy TrEMBL
Match: A0A6J1FHL2 (Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC111445854 PE=3 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 2.8e-227
Identity = 456/736 (61.96%), Postives = 537/736 (72.96%), Query Frame = 0

Query: 1   MDVNGN------------KEGENGGQVVVEMSSSVSS----KENRADSQ----------- 60
           MD+NGN            KE ENG QVVVE+S   +     K+NR DSQ           
Sbjct: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPMDSSIGY 60

Query: 61  ---------------IP---RTLTARKSLKRPILPKPKSRFGEQSYRIDSDMLGENNHGF 120
                          IP    TLT RKSLKR IL KPKSRFGEQS  IDSD   EN   F
Sbjct: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF 120

Query: 121 ----------SSALNTPKE-ADDDDEDIVKIEAFDKEKSKKQKVKKLIKWVGVVCIISCL 180
                      S LNTPKE  ++ DEDI KIE F KEK KK KV  LIKWVG  CII CL
Sbjct: 121 RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180

Query: 181 VASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYLLEKKLLYF 240
           VASLT+ RLK  FLWG+E+W+WCLLATVI+CGMIF++ VM VIV L  RN+LL+KK+ YF
Sbjct: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHRVMNVIVFLIERNFLLQKKVFYF 240

Query: 241 AHGLKKSGRVALWLTLVLVTWASLLNWR-----RSRMAGKILDGVTWSLVCLVIGAVLWL 300
            HGLKKS +V LWLTLVLVTW SL +       RS+++GKILD +TW+L+ L+IGA LWL
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWVSLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300

Query: 301 VKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEGGGEWSGGRLCFKCK 360
           +KTL +KI+  K  MNR+FDRIQ+SIFH++VL+TL  P  +GM E   E + GRL FK K
Sbjct: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360

Query: 361 KTEQEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMSISRILDESCRNFAHAD 420
           K+  +       ID+GK  +++R KVSAWTMK LV+AV+SSEMSIS++LDES +  A  +
Sbjct: 361 KSNHK-----KVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQ-VADGE 420

Query: 421 IADDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRVLPYFGVAETVHIDIKA 480
           I D+ + A   A KIFNNI   GN  IE  +LR  + +E++D VLP F V ET  ID K+
Sbjct: 421 ITDETEVAGYAAFKIFNNIAVPGNDFIEEEDLRRVMIKEEVDLVLPLFEVDETRRIDWKS 480

Query: 481 LTDFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLVTALVIIVTAIIWLLLME 540
           LT++V          +K YKER+T+A+AL DTKTAVKQLNNLVT L+IIVTAIIWLLLME
Sbjct: 481 LTNWV----------LKVYKERKTLAHALKDTKTAVKQLNNLVTVLLIIVTAIIWLLLME 540

Query: 541 IATTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDVGDRCVVDDVPLLVEEMN 600
           IATT++L+FLLSQLAV VFMFGN+CKT FEA++FVFV+HPFDVGDRC V+ VPL+VEEMN
Sbjct: 541 IATTKVLVFLLSQLAVAVFMFGNTCKTIFEALVFVFVMHPFDVGDRCAVEGVPLMVEEMN 600

Query: 601 ILTTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSIGFTTPLERIGAMKERIK 660
           ILTTVFLKLNNEK++YPNSVLATK ITNYYRSP MSDTVEFSIGF TPLERIG MK+RI+
Sbjct: 601 ILTTVFLKLNNEKVYYPNSVLATKSITNYYRSPDMSDTVEFSIGFATPLERIGLMKDRIR 660

Query: 661 RYLKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWPEKTKRRTELMMELKKIF 676
           RYL++NPQHWHP HSVVVKEIEDVNKIK ALY+NHTMNFQDW EK +RRTEL+MELK+IF
Sbjct: 661 RYLEKNPQHWHPNHSVVVKEIEDVNKIKFALYSNHTMNFQDWTEKNRRRTELVMELKRIF 720

BLAST of Spg017003 vs. ExPASy TrEMBL
Match: A0A6J1J491 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111481199 PE=3 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 1.5e-225
Identity = 453/736 (61.55%), Postives = 537/736 (72.96%), Query Frame = 0

Query: 1   MDVNGN------------KEGENGGQVVVEMSSSVSS----KENRADSQ----------- 60
           MD+NGN            KE ENG QVVVE+S   +     K+NR DSQ           
Sbjct: 1   MDLNGNTPLKPIRRSSSQKESENGSQVVVEISRDENGHSVPKQNRVDSQTKEPMDSSIGY 60

Query: 61  ---------------IP---RTLTARKSLKRPILPKPKSRFGEQSYRIDSDMLGENNHGF 120
                          IP    TLT RKSLKR IL KPKSRFGEQS  IDSD   EN   F
Sbjct: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSVIDSDTFEENRLSF 120

Query: 121 ----------SSALNTPKE-ADDDDEDIVKIEAFDKEKSKKQKVKKLIKWVGVVCIISCL 180
                      S LN PKE  ++ D++  KIE F K K KK K   LIKWVG  CII CL
Sbjct: 121 RDQISANSSRRSTLNMPKEHHEEGDDEFFKIEKFKKVKHKKVKAITLIKWVGAFCIIGCL 180

Query: 181 VASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYLLEKKLLYF 240
           VASLT+ RLK  FLWG+E+W+WCLLATVI+CGMIF++ VM VIV L  RN+LL+KK+ YF
Sbjct: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHRVMNVIVFLIERNFLLQKKVFYF 240

Query: 241 AHGLKKSGRVALWLTLVLVTWASLLNWR-----RSRMAGKILDGVTWSLVCLVIGAVLWL 300
            HGLKKS +V LWLTLVLVTW SL +       RS+++GKILD +TW+L+ L+IGA LWL
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWVSLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300

Query: 301 VKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEGGGEWSGGRLCFKCK 360
           +KTL +KI+  K  MNR+FDRIQ+SIFH++VL+TL  P  +GM E   E + GRL FK K
Sbjct: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360

Query: 361 KTEQEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMSISRILDESCRNFAHAD 420
           K++ +       ID+GK  +++R KVSAWTMK LV+AV+SSEMSIS++LDES +  A  +
Sbjct: 361 KSDHK-----KVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQ-VADGE 420

Query: 421 IADDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRVLPYFGVAETVHIDIKA 480
           IAD+M+ A   A KIF+NI   GN  IE  +LR  + +E++D VLP F V ET  ID K+
Sbjct: 421 IADEMEVAGYAAYKIFDNIAVPGNDFIEEEDLRRVMIKEEVDLVLPLFEVDETRRIDWKS 480

Query: 481 LTDFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLVTALVIIVTAIIWLLLME 540
           LT++V          +K YKER+T+A+AL DTKTAVKQLNNLVTAL+IIVTAIIWLLLME
Sbjct: 481 LTNWV----------LKVYKERKTLAHALKDTKTAVKQLNNLVTALLIIVTAIIWLLLME 540

Query: 541 IATTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDVGDRCVVDDVPLLVEEMN 600
           IATT++L+FLLSQLAV VFMFGN+CKT FEA++FVFV+HPFDVGDRC V+ VPL+VEEMN
Sbjct: 541 IATTKVLVFLLSQLAVAVFMFGNTCKTIFEALVFVFVMHPFDVGDRCAVEGVPLMVEEMN 600

Query: 601 ILTTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSIGFTTPLERIGAMKERIK 660
           ILTTVFLKLNNEK++YPNSVLATK ITNYYRSP MSDTVEFSIGF TPLERIG MK+RIK
Sbjct: 601 ILTTVFLKLNNEKVYYPNSVLATKSITNYYRSPDMSDTVEFSIGFATPLERIGLMKDRIK 660

Query: 661 RYLKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWPEKTKRRTELMMELKKIF 676
           RYL++NPQHWHP HSVVVKEIEDVNKIK ALY+NHTMNFQDW EK +RRTEL+MELK+IF
Sbjct: 661 RYLEKNPQHWHPNHSVVVKEIEDVNKIKFALYSNHTMNFQDWTEKNRRRTELVMELKRIF 720

BLAST of Spg017003 vs. ExPASy TrEMBL
Match: A0A0A0L5E6 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_4G647440 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 8.2e-219
Identity = 441/734 (60.08%), Postives = 532/734 (72.48%), Query Frame = 0

Query: 1   MDVNGN---------KEGENGGQVVVEMSSSVSS----KENRADSQ-------------- 60
           MDV+GN         KE  NGG+VVV++S   +     K+NRADSQ              
Sbjct: 1   MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60

Query: 61  ------------IP---RTLTARKSLKRPILPKPKSRFGEQSYRIDSDMLGENNH----- 120
                       IP    TLT R+SL+R  L KPKSRFGEQS  IDSDML E NH     
Sbjct: 61  SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120

Query: 121 ------GFSSALNTPKEADDDDEDIVKIEAFDKEKSKKQKVKKLIKWVGVVCIISCLVAS 180
                   SS LNTPK   + ++D          K KK KVK +IKW+GV CIISCLVAS
Sbjct: 121 QIGATSSRSSNLNTPKAQPEGEDD---GNMGSNAKHKKVKVKTVIKWIGVFCIISCLVAS 180

Query: 181 LTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYLLEKKLLYFAHG 240
           LTV+ LK RFLWGL+VW+WCLLATVI+CG+IF+ WVM V+V L  RN+LL+KK+LYF HG
Sbjct: 181 LTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHG 240

Query: 241 LKKSGRVALWLTLVLVTWASLLNWRR-----SRMAGKILDGVTWSLVCLVIGAVLWLVKT 300
           LKKS +V LWL+LVL TW SL + R      SR+  KILD VTW+L   +IGA LWL+KT
Sbjct: 241 LKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKT 300

Query: 301 LSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEGGGEWSGGRLCFKCKKTE 360
           L +KI+  K  MNR+FDRIQ+S+F ++VL+TL RPP V   E   ++   R CF+ KK  
Sbjct: 301 LLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLV-EDESTAKFRCCRFCFESKKP- 360

Query: 361 QEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMSISRIL-DESCRNFAHADIA 420
               +R   IDMGK   ++R KVSAWTMK LV+AV+SSEMS+S+IL DES R+ A  DI 
Sbjct: 361 ----DRKKVIDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDIT 420

Query: 421 DDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRVLPYFGVAETVHIDIKALT 480
           ++MK A   A +IF N+   GNK IE  +L EF+  E+++ V P+F V +T  ID+KALT
Sbjct: 421 NEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALT 480

Query: 481 DFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLVTALVIIVTAIIWLLLMEIA 540
           ++V          VK Y+ R+T+A+AL DTKTAVKQLNNL+TAL+I+VTA+IWLLLMEIA
Sbjct: 481 NWV----------VKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIA 540

Query: 541 TTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDVGDRCVVDDVPLLVEEMNIL 600
           TT++L+FLL+QLAV  FMFGN+CKT FEA+IFVFV+HPFDVGDRCVVD V LLVEEMNIL
Sbjct: 541 TTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNIL 600

Query: 601 TTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSIGFTTPLERIGAMKERIKRY 660
           TTVFLKLNNEK++YPNSVLATKPITNYYRSP M DTVEFSIGF TP+ERIGAMKE+IKRY
Sbjct: 601 TTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRY 660

Query: 661 LKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWPEKTKRRTELMMELKKIFEE 676
           L+ENPQHW+P H+VVVKEIE+VNKIK+ALYTNHTMNFQDW EK +RRT+L+MELK+IFEE
Sbjct: 661 LEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEE 715

BLAST of Spg017003 vs. ExPASy TrEMBL
Match: A0A1S4DZ48 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103493697 PE=3 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 5.3e-218
Identity = 443/734 (60.35%), Postives = 530/734 (72.21%), Query Frame = 0

Query: 1   MDVNGN---------KEGENGGQVVVEMSSSVSS----KENRADSQ-------------- 60
           MDVNGN         KE ENGG+VVV++S   +     K+NR DSQ              
Sbjct: 1   MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSQTKEPTGSSVGYGNA 60

Query: 61  ------------IP---RTLTARKSLKRPILPKPKSRFGEQSYRIDSDMLGENNH----- 120
                       IP    TLT R+SL+R  L KPKSRFGEQ   IDSDM  E NH     
Sbjct: 61  SQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHVSLRE 120

Query: 121 ------GFSSALNTPKEADDDDEDIVKIEAFDKEKSKKQKVKKLIKWVGVVCIISCLVAS 180
                   SS LNTPK A  + ED   +     EK KK KVK + KW+GV CIISCLVAS
Sbjct: 121 QIGATSSRSSNLNTPK-AQPEGEDGRNMGL--NEKHKKAKVKTVFKWIGVFCIISCLVAS 180

Query: 181 LTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYLLEKKLLYFAHG 240
           LTV  LK RFLWGL+VW+WCLLATVI CG+IF+ W+M V+V L  RN+LL+KK+LYF HG
Sbjct: 181 LTVKPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLIERNFLLKKKVLYFVHG 240

Query: 241 LKKSGRVALWLTLVLVTWASLLNWRR-----SRMAGKILDGVTWSLVCLVIGAVLWLVKT 300
           LKKS +V LWL+LVL TW SL + R      SR+  KILD VTW+L  L+IGA LWL+KT
Sbjct: 241 LKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKT 300

Query: 301 LSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEGGGEWSGGRLCFKCKKTE 360
           L +KI+  K  MNR+FDRIQ+S+F ++VL+TL RPP V   E   ++   R CF+ K++ 
Sbjct: 301 LLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPV-EDESTAKFRCCRFCFESKES- 360

Query: 361 QEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMSISRIL-DESCRNFAHADIA 420
               +R   IDMGK  +++R KVSAWTMK LV+AV+SSEMSIS+IL DES R+ A  DI 
Sbjct: 361 ----DRKKVIDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADGDIT 420

Query: 421 DDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRVLPYFGVAETVHIDIKALT 480
           ++M  A   A +IF N+   GNK IE  +L  F+  E+++ V P+F V +T  ID+KALT
Sbjct: 421 NEMNVAKEAAKEIFKNVALPGNKFIEERDLLAFMIPEEVNLVWPHFEVDKTRKIDMKALT 480

Query: 481 DFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLVTALVIIVTAIIWLLLMEIA 540
           ++V          VK Y+ R+T+A+AL DTKTAVKQLNNL+TAL+IIVTAIIWLLLMEIA
Sbjct: 481 NWV----------VKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVTAIIWLLLMEIA 540

Query: 541 TTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDVGDRCVVDDVPLLVEEMNIL 600
           TT++L+FLL+QLAV  FMFGN+CKT FEA+IFVFV+HPFDVGDRCVVD V LLVEEMNIL
Sbjct: 541 TTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNIL 600

Query: 601 TTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSIGFTTPLERIGAMKERIKRY 660
           TTVFLKLNNEK++YPNSVLATKPITNYYRSP M DTVEFSIGF TP+ERIGAMKE+IKRY
Sbjct: 601 TTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRY 660

Query: 661 LKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWPEKTKRRTELMMELKKIFEE 676
           L+ENPQHW+P H+VVVKEIE+VNKIK+ALYTNHTMNFQDW EK +RRTEL+MELK+IFEE
Sbjct: 661 LEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTELVMELKRIFEE 715

BLAST of Spg017003 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 558.1 bits (1437), Expect = 9.4e-159
Identity = 334/693 (48.20%), Postives = 454/693 (65.51%), Query Frame = 0

Query: 18  EMSSSVSS--KENRADSQIPRTLTARKSLKRPILPKPKSRFGEQSYRIDSDMLGEN---- 77
           E+S  V S  K  RA +Q    LT RKS  R +  KPKSRF + S  +D+ +L E     
Sbjct: 50  EISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEEVREQ 109

Query: 78  -NHGFSSALNTPKEAD-------------------DDDEDIVKIEAFDKEKSKKQKVKKL 137
              GFS +  +P                       D+DE+I K    ++E   K     L
Sbjct: 110 LGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLAL 169

Query: 138 IKWVGVVCIISCLVASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLC 197
           I+    V I+S LVASLT++ LK    WGLEVW+WC+L  VI  GM+ +NW M +IV L 
Sbjct: 170 IESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLI 229

Query: 198 GRNYLLEKKLLYFAHGLKKSGRVALWLTLVLVTWASLLNW--RRSRMAGKILDGVTWSLV 257
             N+LL +K+LYF HGLKKS +V +WL L+LV W  L N   +RS  A K+L  +T +L+
Sbjct: 230 ETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLI 289

Query: 258 CLVIGAVLWLVKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEG-GGE 317
            ++ GA  WLVKTL +KI+     +N +FDRIQDS+FH YVL+TLS  P +   E  G E
Sbjct: 290 SILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGRE 349

Query: 318 WSGGRLCFKCKKTEQEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMS-ISRI 377
            S G L F     +    E+ + IDMGK  +++R KVSAWTM+ L+EAV +S +S IS  
Sbjct: 350 PSTGHLSFATVVKKGTVKEKKV-IDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDT 409

Query: 378 LDESC----RNFAHADIADDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRV 437
           LDE+     +  A  +I  +M+ A A A  +F N+ +     IE  +L  F+ +E++D V
Sbjct: 410 LDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLV 469

Query: 438 LPYF-GVAETVHIDIKALTDFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLV 497
            P F G AET  I  KA T++V          VK Y  R  +A++L DTKTAVKQLN LV
Sbjct: 470 FPLFDGAAETGRITRKAFTEWV----------VKVYTSRRALAHSLNDTKTAVKQLNKLV 529

Query: 498 TALVIIVTAIIWLLLMEIATTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDV 557
           TA++++VT +IWLLL+E+ATT++LLF  +QL  + F+ G++CK  FE+I+FVFV+HP+DV
Sbjct: 530 TAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDV 589

Query: 558 GDRCVVDDVPLLVEEMNILTTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSI 617
           GDRCVVD V +LVEEMN+LTTVFLKLNNEK++YPN+VLATKPI+NY+RSP+M +TVEFSI
Sbjct: 590 GDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSI 649

Query: 618 GFTTPLERIGAMKERIKRYLKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWP 676
            F+TP+ +I  +KERI  YL++NPQHW PVHSVVVKEIE++NK+KMALY++HT+ FQ+  
Sbjct: 650 SFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENR 709

BLAST of Spg017003 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 558.1 bits (1437), Expect = 9.4e-159
Identity = 334/693 (48.20%), Postives = 454/693 (65.51%), Query Frame = 0

Query: 18  EMSSSVSS--KENRADSQIPRTLTARKSLKRPILPKPKSRFGEQSYRIDSDMLGEN---- 77
           E+S  V S  K  RA +Q    LT RKS  R +  KPKSRF + S  +D+ +L E     
Sbjct: 50  EISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEEVREQ 109

Query: 78  -NHGFSSALNTPKEAD-------------------DDDEDIVKIEAFDKEKSKKQKVKKL 137
              GFS +  +P                       D+DE+I K    ++E   K     L
Sbjct: 110 LGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLAL 169

Query: 138 IKWVGVVCIISCLVASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLC 197
           I+    V I+S LVASLT++ LK    WGLEVW+WC+L  VI  GM+ +NW M +IV L 
Sbjct: 170 IESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLI 229

Query: 198 GRNYLLEKKLLYFAHGLKKSGRVALWLTLVLVTWASLLNW--RRSRMAGKILDGVTWSLV 257
             N+LL +K+LYF HGLKKS +V +WL L+LV W  L N   +RS  A K+L  +T +L+
Sbjct: 230 ETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLI 289

Query: 258 CLVIGAVLWLVKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEG-GGE 317
            ++ GA  WLVKTL +KI+     +N +FDRIQDS+FH YVL+TLS  P +   E  G E
Sbjct: 290 SILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGRE 349

Query: 318 WSGGRLCFKCKKTEQEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMS-ISRI 377
            S G L F     +    E+ + IDMGK  +++R KVSAWTM+ L+EAV +S +S IS  
Sbjct: 350 PSTGHLSFATVVKKGTVKEKKV-IDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDT 409

Query: 378 LDESC----RNFAHADIADDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRV 437
           LDE+     +  A  +I  +M+ A A A  +F N+ +     IE  +L  F+ +E++D V
Sbjct: 410 LDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLV 469

Query: 438 LPYF-GVAETVHIDIKALTDFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLV 497
            P F G AET  I  KA T++V          VK Y  R  +A++L DTKTAVKQLN LV
Sbjct: 470 FPLFDGAAETGRITRKAFTEWV----------VKVYTSRRALAHSLNDTKTAVKQLNKLV 529

Query: 498 TALVIIVTAIIWLLLMEIATTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDV 557
           TA++++VT +IWLLL+E+ATT++LLF  +QL  + F+ G++CK  FE+I+FVFV+HP+DV
Sbjct: 530 TAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDV 589

Query: 558 GDRCVVDDVPLLVEEMNILTTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSI 617
           GDRCVVD V +LVEEMN+LTTVFLKLNNEK++YPN+VLATKPI+NY+RSP+M +TVEFSI
Sbjct: 590 GDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSI 649

Query: 618 GFTTPLERIGAMKERIKRYLKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWP 676
            F+TP+ +I  +KERI  YL++NPQHW PVHSVVVKEIE++NK+KMALY++HT+ FQ+  
Sbjct: 650 SFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENR 709

BLAST of Spg017003 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 558.1 bits (1437), Expect = 9.4e-159
Identity = 334/693 (48.20%), Postives = 454/693 (65.51%), Query Frame = 0

Query: 18  EMSSSVSS--KENRADSQIPRTLTARKSLKRPILPKPKSRFGEQSYRIDSDMLGEN---- 77
           E+S  V S  K  RA +Q    LT RKS  R +  KPKSRF + S  +D+ +L E     
Sbjct: 50  EISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEEVREQ 109

Query: 78  -NHGFSSALNTPKEAD-------------------DDDEDIVKIEAFDKEKSKKQKVKKL 137
              GFS +  +P                       D+DE+I K    ++E   K     L
Sbjct: 110 LGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLAL 169

Query: 138 IKWVGVVCIISCLVASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLC 197
           I+    V I+S LVASLT++ LK    WGLEVW+WC+L  VI  GM+ +NW M +IV L 
Sbjct: 170 IESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLI 229

Query: 198 GRNYLLEKKLLYFAHGLKKSGRVALWLTLVLVTWASLLNW--RRSRMAGKILDGVTWSLV 257
             N+LL +K+LYF HGLKKS +V +WL L+LV W  L N   +RS  A K+L  +T +L+
Sbjct: 230 ETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLI 289

Query: 258 CLVIGAVLWLVKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEG-GGE 317
            ++ GA  WLVKTL +KI+     +N +FDRIQDS+FH YVL+TLS  P +   E  G E
Sbjct: 290 SILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGRE 349

Query: 318 WSGGRLCFKCKKTEQEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMS-ISRI 377
            S G L F     +    E+ + IDMGK  +++R KVSAWTM+ L+EAV +S +S IS  
Sbjct: 350 PSTGHLSFATVVKKGTVKEKKV-IDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDT 409

Query: 378 LDESC----RNFAHADIADDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRV 437
           LDE+     +  A  +I  +M+ A A A  +F N+ +     IE  +L  F+ +E++D V
Sbjct: 410 LDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLV 469

Query: 438 LPYF-GVAETVHIDIKALTDFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLV 497
            P F G AET  I  KA T++V          VK Y  R  +A++L DTKTAVKQLN LV
Sbjct: 470 FPLFDGAAETGRITRKAFTEWV----------VKVYTSRRALAHSLNDTKTAVKQLNKLV 529

Query: 498 TALVIIVTAIIWLLLMEIATTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDV 557
           TA++++VT +IWLLL+E+ATT++LLF  +QL  + F+ G++CK  FE+I+FVFV+HP+DV
Sbjct: 530 TAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDV 589

Query: 558 GDRCVVDDVPLLVEEMNILTTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSI 617
           GDRCVVD V +LVEEMN+LTTVFLKLNNEK++YPN+VLATKPI+NY+RSP+M +TVEFSI
Sbjct: 590 GDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSI 649

Query: 618 GFTTPLERIGAMKERIKRYLKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWP 676
            F+TP+ +I  +KERI  YL++NPQHW PVHSVVVKEIE++NK+KMALY++HT+ FQ+  
Sbjct: 650 SFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENR 709

BLAST of Spg017003 vs. TAIR 10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9 )

HSP 1 Score: 510.0 bits (1312), Expect = 2.9e-144
Identity = 296/680 (43.53%), Postives = 433/680 (63.68%), Query Frame = 0

Query: 35  PRTLTARKSLKRPILPKPKSRFGE-QSYRIDS------------------------DMLG 94
           P  L  RKSL R I  KPKSRFGE QS+R DS                        D   
Sbjct: 75  PEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQFGAGSFARGSFDRAS 134

Query: 95  ENNHG----FSSALN-TPKEADDDDEDIVKIEAFDKEKSKKQKVKKLIKWVGVVCIISCL 154
            NN       S+AL+   +E  D++E+I K     + K    K    ++ V  + I+  L
Sbjct: 135 PNNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGMKPLAFLELVVFMAILGAL 194

Query: 155 VASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYLLEKKLLYF 214
           + SLT+D + K  +WGLE W+WC+L  V + GM+ +NW M  +V +  +NYLL KK+LYF
Sbjct: 195 IVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHFVVFIIEKNYLLRKKVLYF 254

Query: 215 AHGLKKSGRVALWLTLVLVTWASLLNW--RRSRMAGKILDGVTWSLVCLVIGAVLWLVKT 274
            HGLKK+ +V +W +LVL+ W  L +   +R+R   + LD +TW++V L++G++L+LVKT
Sbjct: 255 VHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKTKRFLDFITWTIVSLLVGSILFLVKT 314

Query: 275 LSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTLSRPPSVGMGEGGGEW-SGGRLCFKCKKT 334
            ++K++  K  +  +F+RIQ+S+FH YVL+TLS PP +   E  G   S G L F   K 
Sbjct: 315 FALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEEAENVGRVPSTGHLSFTRTKD 374

Query: 335 EQEHGERVMWIDMGKAQRVERHKVSAWTMKRLVEAVSSSEMS-ISRILDE--SCRNFAHA 394
            +   ++V  IDMGK  R+++ KVSAWTM+ L+EAV +S +S IS  LDE  + +     
Sbjct: 375 GKVKDKKV--IDMGKVHRMKQEKVSAWTMRVLIEAVGTSGISTISSTLDEVNNKKERTDK 434

Query: 395 DIADDMKHAAAVASKIFNNIDRDGNKIIERANLREFLSEEQIDRVLPYFGVAETVHIDIK 454
           +I ++M+ A A A  +FNN+ +  +  IE  +L  F+ +E++D VLP    A+T  I  K
Sbjct: 435 EITNEME-AVAAAYDVFNNVAKPNHNYIEEDDLLRFMIKEEVDLVLPLIEDADTGKITRK 494

Query: 455 ALTDFVTFSVRSEAVMVKAYKERETIAYALTDTKTAVKQLNNLVTALVIIVTAIIWLLLM 514
             T++V          V  Y  R+TI ++L DTKTAVKQL+ L+T ++ ++T I+W++L+
Sbjct: 495 TFTEWV----------VNVYTSRKTIGHSLNDTKTAVKQLDKLITGILTVITFIVWMVLL 554

Query: 515 EIATTQLLLFLLSQLAVVVFMFGNSCKTTFEAIIFVFVIHPFDVGDRCVVDDVPLLVEEM 574
           +IA+T+LLL   SQ   + FM G++CK  FE+ +FVFV+HP+DVGDRCVVD V LLVEE+
Sbjct: 555 DIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCVVDGVMLLVEEI 614

Query: 575 NILTTVFLKLNNEKMWYPNSVLATKPITNYYRSPHMSDTVEFSIGFTTPLERIGAMKERI 634
           ++LTTVFLK++NEK++YPNSVL +KPI+N+YRSP M D V+F I F+TP E+IG +K +I
Sbjct: 615 DLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTPAEKIGCLKGKI 674

Query: 635 KRYLKENPQHWHPVHSVVVKEIEDVNKIKMALYTNHTMNFQDWPEKTKRRTELMMELKKI 679
             YL  N QHW+P   V+V+ IE++NK+ + +   HT+NFQ + EK+ RRT L++ +K+I
Sbjct: 675 GEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVEKSLRRTALIIAIKRI 734

BLAST of Spg017003 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 399.8 bits (1026), Expect = 4.3e-111
Identity = 257/722 (35.60%), Postives = 398/722 (55.12%), Query Frame = 0

Query: 17  VEMSSSVSSKENRAD-SQIPRTLTARKSLKRPILPKPK--SRFGEQSYRIDSDML----- 76
           V+   S   +ENR D S++ +  + R +++   L K K  SR  +       DM+     
Sbjct: 148 VKDGDSSEDEENRVDGSEVVKCTSNRSTMRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTP 207

Query: 77  --GENNHGFSSALNTP--------KEADDDDEDIVKIEAFDKEKSKKQKVKKLIKWVGVV 136
             G  N GFS     P        K+ +++++   + +  +  + +K  V  +I+W+ ++
Sbjct: 208 RSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLI 267

Query: 137 CIISCLVASLTVDRLKKRFLWGLEVWEWCLLATVIICGMIFSNWVMEVIVNLCGRNYLLE 196
            II+ L+ SL +  L+ + LW L +W+W ++  V+ICG + S+W++++ V     N+L  
Sbjct: 268 LIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWR 327

Query: 197 KKLLYFAHGLKKSGRVALWLTLVLVTWASLLNWRRSR-MAGKILDGVTWSLVCLVIGAVL 256
           KK+LYF +G++K  +  LWL LVL+ W  L + +  R M   +L  VT  L+CL++  ++
Sbjct: 328 KKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVII 387

Query: 257 WLVKTLSMKIVMHKLRMNRYFDRIQDSIFHYYVLRTL----------------------- 316
           WL+KTL +K++     M+ YFDRIQ+S+F  YV+ TL                       
Sbjct: 388 WLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFE 447

Query: 317 --------------SRPPSVGMGEGGGEWSGGRL----CFKCKKTEQEHGERVMWIDMGK 376
                         S PP V +G G  + S  R+          +++E GE  + ID   
Sbjct: 448 IVGRKLSPLGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRID--H 507

Query: 377 AQRVERHKVSAWTMKRLVEAVSSSEMSI--SRILDESCRNFAHADIADDMKHAAAVASKI 436
            QR+    VSAW MK+L+  +    +S    +I D + +    A        A   A KI
Sbjct: 508 LQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKI 567

Query: 437 FNNIDRDGNKIIERANLREFLSEEQIDRVLPYF-GVAETVHIDIKALTDFVTFSVRSEAV 496
           F N+   G++ I   +   FLSE++ +R +  F G +E   I    L ++V         
Sbjct: 568 FQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWV--------- 627

Query: 497 MVKAYKERETIAYALTDTKTAVKQLNNLVTALVIIVTAIIWLLLMEIATTQLLLFLLSQL 556
            V A++ER  +A  L DTKTAV +L+ +V  LV IV  IIWLL++ IATT+ LL + SQL
Sbjct: 628 -VNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQL 687

Query: 557 AVVVFMFGNSCKTTFEAIIFVFVIHPFDVGDRCVVDDVPLLVEEMNILTTVFLKLNNEKM 616
            +VVF+FGNSCKT FEA+IFVFV+HPFDVGDRC +D V ++VEEMNILTTVFL+ +N+K+
Sbjct: 688 LLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKI 747

Query: 617 WYPNSVLATKPITNYYRSPHMSDTVEFSIGFTTPLERIGAMKERIKRYLKENPQHWHPVH 676
            YPNS+L TKPI NYYRSP M D +EF +   TP E+  A+++RI  Y+     HWHP  
Sbjct: 748 VYPNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSP 807

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138170.15.6e-23062.40mechanosensitive ion channel protein 10 [Momordica charantia] >XP_022138178.1 me... [more]
XP_023524086.11.2e-22762.09mechanosensitive ion channel protein 10-like [Cucurbita pepo subsp. pepo] >XP_02... [more]
XP_022940136.15.8e-22761.96mechanosensitive ion channel protein 10-like [Cucurbita moschata] >XP_022940137.... [more]
XP_022982339.13.2e-22561.55mechanosensitive ion channel protein 10-like [Cucurbita maxima] >XP_022982340.1 ... [more]
KAG6608342.11.5e-22259.71Mechanosensitive ion channel protein 10, partial [Cucurbita argyrosperma subsp. ... [more]
Match NameE-valueIdentityDescription
Q9LYG91.3e-15748.20Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10... [more]
Q84M974.2e-14343.53Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 P... [more]
Q9LPG36.1e-11035.60Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 P... [more]
Q9LH741.3e-10936.33Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 P... [more]
Q9SYM17.4e-10834.73Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 P... [more]
Match NameE-valueIdentityDescription
A0A6J1C8Z82.7e-23062.40Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111009... [more]
A0A6J1FHL22.8e-22761.96Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC1114458... [more]
A0A6J1J4911.5e-22561.55Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111481199... [more]
A0A0A0L5E68.2e-21960.08Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_4G647440 ... [more]
A0A1S4DZ485.3e-21860.35Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103493697 PE=... [more]
Match NameE-valueIdentityDescription
AT5G12080.19.4e-15948.20mechanosensitive channel of small conductance-like 10 [more]
AT5G12080.29.4e-15948.20mechanosensitive channel of small conductance-like 10 [more]
AT5G12080.39.4e-15948.20mechanosensitive channel of small conductance-like 10 [more]
AT5G19520.12.9e-14443.53mechanosensitive channel of small conductance-like 9 [more]
AT1G53470.14.3e-11135.60mechanosensitive channel of small conductance-like 4 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPIRSFPIRSF017209Memb_At2g17000coord: 8..678
e-value: 9.6E-163
score: 541.5
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPANTHERPTHR31618MECHANOSENSITIVE ION CHANNEL PROTEIN 5coord: 13..677
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 460..665
e-value: 7.6E-21
score: 74.6
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3D2.30.30.60coord: 519..568
e-value: 1.4E-9
score: 39.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..39
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..58
NoneNo IPR availablePANTHERPTHR31618:SF7MECHANOSENSITIVE ION CHANNEL PROTEINcoord: 13..677
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 366..401
score: 9.073802
IPR010920LSM domain superfamilySUPERFAMILY50182Sm-like ribonucleoproteinscoord: 508..568

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg017003.1Spg017003.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006820 anion transport
biological_process GO:0050982 detection of mechanical stimulus
biological_process GO:0055085 transmembrane transport
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0008381 mechanosensitive ion channel activity