Spg016878 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016878 vs. NCBI nr
Match: XP_022148154.1 (probable carboxylesterase 17 [Momordica charantia])

HSP 1 Score: 555.1 bits (1429), Expect = 3.9e-154
Identity = 269/310 (86.77%), Postives = 292/310 (94.19%), Query Frame = 0

Query: 1   MAMIGEAPGYLQVYSDGSVRRSIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLPD- 60
           MA+IGEAPGYL+VYSDG+VRR IP+VV S A QSSKN+KFKDV+IDP++ VTGRLFLPD 
Sbjct: 1   MALIGEAPGYLKVYSDGTVRRLIPDVVASMATQSSKNFKFKDVVIDPRKAVTGRLFLPDA 60

Query: 61  IADAPGRLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAA 120
            ADA GRLPV+VYFHGGGFCIGSTTWIGYH+FLGDLAAA+++VILSVDYRLAPENRLPAA
Sbjct: 61  AADAAGRLPVVVYFHGGGFCIGSTTWIGYHHFLGDLAAASQSVILSVDYRLAPENRLPAA 120

Query: 121 YEDCYSSLEWLGHQASSEPWLQNADPSRVFLSGDSAGGNIVHHVAVKTIKSNIPNLTIKG 180
           YEDCYS+LEWLGHQASSEPWLQ AD S VFLSGDSAGGNIVH+VA+KTIKSNI N+TIKG
Sbjct: 121 YEDCYSALEWLGHQASSEPWLQQADLSHVFLSGDSAGGNIVHNVAIKTIKSNISNMTIKG 180

Query: 181 LLLIHPYFGSKKRTEKEKEEGAAGLVEMNDMFWRLSIPEGRDRDYFGCNYENAQVTKEEW 240
           LLLIHPYFGS++RTEKEK EGA G VEMNDMFWRLSIPEGR+RDYFGCN+ENAQVT+EEW
Sbjct: 181 LLLIHPYFGSEERTEKEKAEGAEGHVEMNDMFWRLSIPEGRNRDYFGCNFENAQVTEEEW 240

Query: 241 SQFPNVVVFVAGLDFLKERGELYAKFLQQMKVKEVKLVEAADESHVFHVFHPESEATRLL 300
           SQFP V VFVAGLDFLKERG LYAKFLQQMKVKEVKL+EAADESHVFHVFHPESEATRLL
Sbjct: 241 SQFPGVAVFVAGLDFLKERGVLYAKFLQQMKVKEVKLLEAADESHVFHVFHPESEATRLL 300

Query: 301 QKQMSQFMKS 310
           QKQMSQFMKS
Sbjct: 301 QKQMSQFMKS 310

BLAST of Spg016878 vs. NCBI nr
Match: XP_038896586.1 (probable carboxylesterase 17 [Benincasa hispida])

HSP 1 Score: 520.4 bits (1339), Expect = 1.1e-143
Identity = 250/311 (80.39%), Postives = 277/311 (89.07%), Query Frame = 0

Query: 1   MAMIGEAPGYLQVYSDGSVRRSIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLPDI 60
           MA+IGE PGY +VYSDGSVRRSIPEVVVST  QSSKNY FKDV+ID K+ VTGRLF P++
Sbjct: 1   MALIGEVPGYFEVYSDGSVRRSIPEVVVSTGTQSSKNYNFKDVVIDSKKLVTGRLFRPNV 60

Query: 61  ADAPGRLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAAY 120
            ++  +LP+LVYFHGGGFCIGSTTWIGYHNFL DLA A+ ++ILSVDYRLAPENRLPAAY
Sbjct: 61  TNSTTKLPILVYFHGGGFCIGSTTWIGYHNFLKDLAIASESIILSVDYRLAPENRLPAAY 120

Query: 121 EDCYSSLEWLGHQASSEPWLQNADPSRVFLSGDSAGGNIVHHVAVKTIKSNIPNLTIKGL 180
           EDCY+SLEWL +Q SSEPWLQN D SRVFLSGDSAGGNIVH+VA+K+IKSNI NLTIKGL
Sbjct: 121 EDCYNSLEWLSYQESSEPWLQNVDLSRVFLSGDSAGGNIVHNVAIKSIKSNISNLTIKGL 180

Query: 181 LLIHPYFGSKKRTEKEKEEGAAGLVEMNDMFWRLSIPEGRDRDYFGCNYEN--AQVTKEE 240
           LLIHPYFGS+KRTE EK  G  G VEMNDMFWRLSIP G DRDYFGCNYE    QV+KEE
Sbjct: 181 LLIHPYFGSEKRTENEKGRGVEGFVEMNDMFWRLSIPTGCDRDYFGCNYETNAQQVSKEE 240

Query: 241 WSQFPNVVVFVAGLDFLKERGELYAKFLQQMKVKEVKLVEAADESHVFHVFHPESEATRL 300
           W+ FPNVVVFVA LDFLKERGELYAKFLQ+MKVKEVKL+EAA+ESHV+HVFHP+SEATRL
Sbjct: 241 WNHFPNVVVFVAALDFLKERGELYAKFLQKMKVKEVKLIEAANESHVYHVFHPQSEATRL 300

Query: 301 LQKQMSQFMKS 310
           LQ+QMS FMKS
Sbjct: 301 LQEQMSHFMKS 311

BLAST of Spg016878 vs. NCBI nr
Match: XP_023536266.1 (probable carboxylesterase 17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 513.5 bits (1321), Expect = 1.3e-141
Identity = 248/313 (79.23%), Postives = 279/313 (89.14%), Query Frame = 0

Query: 1   MAMIGEAPGYLQVYSDGSVRRSIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLPDI 60
           MA+IGEAPG+L+VYSDGSVRR IPEVVVST  QSSKNYKFKDV+IDPK+ VTGRLFLP++
Sbjct: 13  MALIGEAPGFLEVYSDGSVRRLIPEVVVSTDNQSSKNYKFKDVVIDPKKPVTGRLFLPNV 72

Query: 61  -ADAPGRLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAA 120
             +   +LP+LVYFHGGGFCIGSTTW+GYH+FLG LA A+  VILS+DYRLAPENRLPAA
Sbjct: 73  TGNHVAKLPILVYFHGGGFCIGSTTWMGYHHFLGGLAVASNCVILSIDYRLAPENRLPAA 132

Query: 121 YEDCYSSLEWLGHQASS-EPWLQNADPSRVFLSGDSAGGNIVHHVAVKTIKSNIPNLTIK 180
           YEDCYSSLEWL HQAS  EPWLQNAD SRVFLSGDSAGGNIVHHVAVKT+K+NI N+T+K
Sbjct: 133 YEDCYSSLEWLAHQASCFEPWLQNADLSRVFLSGDSAGGNIVHHVAVKTMKTNISNMTLK 192

Query: 181 GLLLIHPYFGSKKRTEKEKEEGAAGLVEMNDMFWRLSIPEGRDRDYFGCNYEN--AQVTK 240
           GL+LIHPYFGS+KRTEKEK EG  G VEMNDMFWRLSIP+G +RDYFGCNYEN   +++K
Sbjct: 193 GLMLIHPYFGSEKRTEKEKAEGVEGFVEMNDMFWRLSIPKGSNRDYFGCNYENNTQEMSK 252

Query: 241 EEWSQFPNVVVFVAGLDFLKERGELYAKFLQQMKVKEVKLVEAADESHVFHVFHPESEAT 300
           EEWS+FPNVVVFVAGLDFLKERGELY  FLQQ +VK VKL+EA DESHVFHVF PESE+T
Sbjct: 253 EEWSKFPNVVVFVAGLDFLKERGELYVNFLQQKRVKVVKLIEAVDESHVFHVFRPESEST 312

Query: 301 RLLQKQMSQFMKS 310
           RLLQ QM+QFM +
Sbjct: 313 RLLQMQMTQFMNT 325

BLAST of Spg016878 vs. NCBI nr
Match: XP_022936542.1 (probable carboxylesterase 17 isoform X1 [Cucurbita moschata])

HSP 1 Score: 511.9 bits (1317), Expect = 3.8e-141
Identity = 247/313 (78.91%), Postives = 279/313 (89.14%), Query Frame = 0

Query: 1   MAMIGEAPGYLQVYSDGSVRRSIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLPDI 60
           MA+IGEAPG+L+VYSDGSVRR IPEVVVST  QSSKNYKFKDV+IDPK+ VTGRLFLP++
Sbjct: 14  MALIGEAPGFLEVYSDGSVRRLIPEVVVSTDNQSSKNYKFKDVVIDPKKPVTGRLFLPNV 73

Query: 61  -ADAPGRLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAA 120
             +   +LP+LVYFHGGGFCIGSTTW+GYH+FLG LA A++ VILS+DYRLAPENRLPAA
Sbjct: 74  TGNHVAKLPILVYFHGGGFCIGSTTWMGYHHFLGGLAVASKCVILSIDYRLAPENRLPAA 133

Query: 121 YEDCYSSLEWLGHQASS-EPWLQNADPSRVFLSGDSAGGNIVHHVAVKTIKSNIPNLTIK 180
           YEDCYSSLEWL HQAS  EPWLQNAD SRVFLSGDSAGGNIVHHVAVKT+K+NI N+T+K
Sbjct: 134 YEDCYSSLEWLAHQASCFEPWLQNADLSRVFLSGDSAGGNIVHHVAVKTMKTNISNMTLK 193

Query: 181 GLLLIHPYFGSKKRTEKEKEEGAAGLVEMNDMFWRLSIPEGRDRDYFGCNYEN--AQVTK 240
           GL+LIHPYFGS+KRTEKEK EG  G VEMNDMFWRLSIP+G +RDYFGCNYEN   +++ 
Sbjct: 194 GLMLIHPYFGSEKRTEKEKAEGVEGFVEMNDMFWRLSIPKGSNRDYFGCNYENNTQEMST 253

Query: 241 EEWSQFPNVVVFVAGLDFLKERGELYAKFLQQMKVKEVKLVEAADESHVFHVFHPESEAT 300
           EEWS+FPNVVVFVAGLDFLKERGELY  FLQQ +VK VKL+EA DESHVFHVF PESE+T
Sbjct: 254 EEWSKFPNVVVFVAGLDFLKERGELYVNFLQQKRVKVVKLIEAVDESHVFHVFRPESEST 313

Query: 301 RLLQKQMSQFMKS 310
           RLLQ QM+QFM +
Sbjct: 314 RLLQMQMTQFMNT 326

BLAST of Spg016878 vs. NCBI nr
Match: XP_022936543.1 (probable carboxylesterase 17 isoform X2 [Cucurbita moschata])

HSP 1 Score: 511.9 bits (1317), Expect = 3.8e-141
Identity = 247/313 (78.91%), Postives = 279/313 (89.14%), Query Frame = 0

Query: 1   MAMIGEAPGYLQVYSDGSVRRSIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLPDI 60
           MA+IGEAPG+L+VYSDGSVRR IPEVVVST  QSSKNYKFKDV+IDPK+ VTGRLFLP++
Sbjct: 1   MALIGEAPGFLEVYSDGSVRRLIPEVVVSTDNQSSKNYKFKDVVIDPKKPVTGRLFLPNV 60

Query: 61  -ADAPGRLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAA 120
             +   +LP+LVYFHGGGFCIGSTTW+GYH+FLG LA A++ VILS+DYRLAPENRLPAA
Sbjct: 61  TGNHVAKLPILVYFHGGGFCIGSTTWMGYHHFLGGLAVASKCVILSIDYRLAPENRLPAA 120

Query: 121 YEDCYSSLEWLGHQASS-EPWLQNADPSRVFLSGDSAGGNIVHHVAVKTIKSNIPNLTIK 180
           YEDCYSSLEWL HQAS  EPWLQNAD SRVFLSGDSAGGNIVHHVAVKT+K+NI N+T+K
Sbjct: 121 YEDCYSSLEWLAHQASCFEPWLQNADLSRVFLSGDSAGGNIVHHVAVKTMKTNISNMTLK 180

Query: 181 GLLLIHPYFGSKKRTEKEKEEGAAGLVEMNDMFWRLSIPEGRDRDYFGCNYEN--AQVTK 240
           GL+LIHPYFGS+KRTEKEK EG  G VEMNDMFWRLSIP+G +RDYFGCNYEN   +++ 
Sbjct: 181 GLMLIHPYFGSEKRTEKEKAEGVEGFVEMNDMFWRLSIPKGSNRDYFGCNYENNTQEMST 240

Query: 241 EEWSQFPNVVVFVAGLDFLKERGELYAKFLQQMKVKEVKLVEAADESHVFHVFHPESEAT 300
           EEWS+FPNVVVFVAGLDFLKERGELY  FLQQ +VK VKL+EA DESHVFHVF PESE+T
Sbjct: 241 EEWSKFPNVVVFVAGLDFLKERGELYVNFLQQKRVKVVKLIEAVDESHVFHVFRPESEST 300

Query: 301 RLLQKQMSQFMKS 310
           RLLQ QM+QFM +
Sbjct: 301 RLLQMQMTQFMNT 313

BLAST of Spg016878 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.1e-50
Identity = 121/305 (39.67%), Postives = 169/305 (55.41%), Query Frame = 0

Query: 3   MIGEAPGYLQVYSDGSVRR-SIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLPDIA 62
           ++ E  G ++V++DG V R  I  +V  T   SSK   F    I        R+++PD A
Sbjct: 28  VVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKATAFD---IKLSNDTWTRVYIPDAA 87

Query: 63  DAPG--RLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAA 122
            A     LP+LVYFHGGGFC+GS  W  YH+FL  LA  AR VI+SV+YRLAPE+RLPAA
Sbjct: 88  AASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHRLPAA 147

Query: 123 YEDCYSSLEWLGHQASS-----EPWLQNADPSRVFLSGDSAGGNIVHHVAVKTIKSN--I 182
           Y+D  + + WL  Q  S       WL   + S VFL+GDSAG NI + VAV+ + S    
Sbjct: 148 YDDGVNVVSWLVKQQISTGGGYPSWLSKCNLSNVFLAGDSAGANIAYQVAVRIMASGKYA 207

Query: 183 PNLTIKGLLLIHPYFGSKKRTEKEKEE----GAAGLVEMNDMFWRLSIPEGRDRDYFGCN 242
             L +KG++LIHP+FG + RT  EK++     +A  +  +D +WRL++P G  RD+  CN
Sbjct: 208 NTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRGASRDHPWCN 267

Query: 243 YENAQVTKEEWSQFPNVVVFVAGLDFLKERGELYAKFLQQMKVKEVKLVEAADESHVFHV 294
                +     ++ P  +VF+A  D LKER     K ++    K V+ +      H FH+
Sbjct: 268 ----PLMSSAGAKLPTTMVFMAEFDILKERNLEMCKVMRS-HGKRVEGIVHGGVGHAFHI 324

BLAST of Spg016878 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 7.9e-49
Identity = 122/328 (37.20%), Postives = 184/328 (56.10%), Query Frame = 0

Query: 1   MAMIGEAP-------GYLQVYSDGSVRR--SIPEVVVSTAKQSSKNYKFKDVIIDPKRRV 60
           M  +GE P       G LQ+ S+G+V R  SI  +      ++++   FKD I      +
Sbjct: 1   MGSLGEEPQVAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNL 60

Query: 61  TGRLFLPDIADAPGRLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLA 120
             RL+ P  A     LPV+V+FHGGGFC GS +W  +HNF   LA++   +++S DYRLA
Sbjct: 61  HLRLYKPISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLA 120

Query: 121 PENRLPAAYEDCYSSLEWLGHQASSE---PWLQ---NADPSRVFLSGDSAGGNIVHHVAV 180
           PE+RLPAA+ED  + L WL  QA S+    W +   + D  RVF+ GDS+GGNI H +AV
Sbjct: 121 PEHRLPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAV 180

Query: 181 KTIKSNI--PNLTIKGLLLIHPYFGSKKRTEKEKEEGAAGL-VEMNDMFWRLSIPEGRDR 240
           +    +I    + ++G +L+ P+FG ++RT  E     A L +++ D FWRLS+P G  R
Sbjct: 181 RFGSGSIELTPVRVRGYVLMGPFFGGEERTNSENGPSEALLSLDLLDKFWRLSLPNGATR 240

Query: 241 DYFGCN-YENAQVTKEEWSQFPNVVVFVAGLDFLKERGELYAKFLQQMKVKEVKLVEAAD 300
           D+   N +     T E  S  P ++V V G + L++R + YA  L++M  K V  +E  +
Sbjct: 241 DHHMANPFGPTSPTLESISLEP-MLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFEN 300

Query: 301 ESHVFHVFHPESEATRLLQKQMSQFMKS 310
           + H F+  +P SEA   + + +  FM +
Sbjct: 301 KEHGFYSNYPSSEAAEQVLRIIGDFMNN 327

BLAST of Spg016878 vs. ExPASy Swiss-Prot
Match: I4DST8 (Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA1 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.3e-47
Identity = 115/305 (37.70%), Postives = 170/305 (55.74%), Query Frame = 0

Query: 10  YLQVYSDGSVRRSIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLPDIADAPG-RLP 69
           +L +Y  G + R +   V+    + +     KDV+IDP   V+ RL+LP++ D P  +LP
Sbjct: 90  FLIIYKSGRIERFLGTTVIPACPEVAT----KDVVIDPATGVSVRLYLPNVVDLPSKKLP 149

Query: 70  VLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAAYEDCYSSLE 129
           VLVYFHGGGF I +T    YHN+L  LAA A  +I+S++YRLAPE  LPA+Y+DC +   
Sbjct: 150 VLVYFHGGGFVIENTGSPNYHNYLTLLAAKAGVLIVSINYRLAPEYPLPASYDDCMAGFN 209

Query: 130 W-LGHQA--SSEPWL-QNADPSRVFLSGDSAGGNIVHHVAVKTIKSNIPNLTIKGLLLIH 189
           W + H A  + EPWL Q+ D S++ LSGDSAGGN+ H+VA++          I+G+ ++H
Sbjct: 210 WVVSHSAGPALEPWLAQHGDFSQILLSGDSAGGNVTHYVAMRADAG-----VIEGVAIVH 269

Query: 190 PYFGSKKRTEKEKEEGAAGLVEMNDMFWRLSIPEGRDRDYFGCNYENAQVTKEEWSQFPN 249
           PYF   +    E  + A   +E +D  WRL+ P+    D    N            +   
Sbjct: 270 PYFLGSEPVGNEINDPAN--IEFHDKLWRLAAPDTEGLDDPLINPVAPGAPSLAGLKCKR 329

Query: 250 VVVFVAGLDFLKERGELYAKFLQQMKVK-EVKLVEAADESHVFHVFHPESEATRLLQKQM 309
            VVFVAG DFL ERG +Y + L +   + E +LV+     HVFH+     + +  +  ++
Sbjct: 330 AVVFVAGNDFLVERGRMYYEALVKSGWRGEAELVQHEGVGHVFHLSDYSGDISVAMMTKL 383

BLAST of Spg016878 vs. ExPASy Swiss-Prot
Match: I4DST9 (Tuliposide A-converting enzyme 2, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA2 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.2e-46
Identity = 117/307 (38.11%), Postives = 171/307 (55.70%), Query Frame = 0

Query: 10  YLQVYSDGSVRRSIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLPDIADAPG-RLP 69
           +L +Y  G + R +   V+    + +     KDV+IDP   V+ RL+LP++ D P  +LP
Sbjct: 87  FLIIYKSGRIERFLGTTVIPACPEVAT----KDVVIDPATGVSVRLYLPNVVDLPSKKLP 146

Query: 70  VLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAAYEDCYSSLE 129
           VLVYFHGGGF I +T    YHN+L  LAA A  +I+S++YRLAPE  LPA+Y+DC +   
Sbjct: 147 VLVYFHGGGFVIENTGSPNYHNYLTLLAAKAGVLIVSINYRLAPEYPLPASYDDCMAGFN 206

Query: 130 W-LGHQA--SSEPWL-QNADPSRVFLSGDSAGGNIVHHVAVKTIKSNIPNLTIKGLLLIH 189
           W + H A  + EPWL Q+ D S++ LSGDSAGGN+ H+VA++          I+G+ ++H
Sbjct: 207 WVVSHSAGPALEPWLAQHGDFSQILLSGDSAGGNVTHYVAMRADAG-----VIEGVAIVH 266

Query: 190 PYFGSKKRTEKEKEEGAAGLVEMNDMFWRLSIP--EGRDRDYFGCNYENAQVTKEEWSQF 249
           PYF   +    E  + A   +E +D  WRL+ P  EG D          A +      + 
Sbjct: 267 PYFLGSEPVGNEINDPAN--IEFHDKLWRLAAPDTEGLDDPLINPVAPGAPIL--AGLKC 326

Query: 250 PNVVVFVAGLDFLKERGELYAKFLQQMK-VKEVKLVEAADESHVFHVFHPESEATRLLQK 309
              VVFVAG DFL ERG +Y + L +     E +LV+     HVFH+     + +  +  
Sbjct: 327 KRAVVFVAGNDFLVERGRMYYEALVKSGWGGEAELVQHEGVGHVFHLSDYSGDISVAMMT 380

BLAST of Spg016878 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.8e-46
Identity = 98/261 (37.55%), Postives = 146/261 (55.94%), Query Frame = 0

Query: 3   MIGEAPGYLQVYSDGSVRRSIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLP--DI 62
           ++ E  G ++VY DG V RS     V  +          DV+ID    V  RL++P    
Sbjct: 23  VVDEVEGLIKVYKDGHVERSQLLPCVDPSLPLELGVTCSDVVIDKLTNVWARLYVPMTTT 82

Query: 63  ADAPGRLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAAY 122
             +  +LP++VYFHGGGFC+GS +W+ YH FL  L+A +R +++SV+YRLAPEN LPAAY
Sbjct: 83  KSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLPAAY 142

Query: 123 EDCYSSLEWLGHQASSEPWLQNADPSRVFLSGDSAGGNIVHHVAVKTIKSNIPNLTIKGL 182
           ED  +++ WL    +   W +  D  R+FL+GDSAGGNI   VA +        L I+G 
Sbjct: 143 EDGVNAILWLNKARNDNLWAKQCDFGRIFLAGDSAGGNIAQQVAARLASPEDLALKIEGT 202

Query: 183 LLIHPYFGSKKRTEKEKEEG----AAGLVEMNDMFWRLSIPEGRDRDYFGCNYENAQVTK 242
           +LI P++  ++RTE E+  G    A   +  +D +WR+S+P G +R++  C      +  
Sbjct: 203 ILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANREHPYCKPVKMIIKS 262

Query: 243 EEWSQFPNVVVFVAGLDFLKE 258
              S     +V VA +D L +
Sbjct: 263 ---STVTRTLVCVAEMDLLMD 280

BLAST of Spg016878 vs. ExPASy TrEMBL
Match: A0A6J1D353 (probable carboxylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111016870 PE=4 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 1.9e-154
Identity = 269/310 (86.77%), Postives = 292/310 (94.19%), Query Frame = 0

Query: 1   MAMIGEAPGYLQVYSDGSVRRSIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLPD- 60
           MA+IGEAPGYL+VYSDG+VRR IP+VV S A QSSKN+KFKDV+IDP++ VTGRLFLPD 
Sbjct: 1   MALIGEAPGYLKVYSDGTVRRLIPDVVASMATQSSKNFKFKDVVIDPRKAVTGRLFLPDA 60

Query: 61  IADAPGRLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAA 120
            ADA GRLPV+VYFHGGGFCIGSTTWIGYH+FLGDLAAA+++VILSVDYRLAPENRLPAA
Sbjct: 61  AADAAGRLPVVVYFHGGGFCIGSTTWIGYHHFLGDLAAASQSVILSVDYRLAPENRLPAA 120

Query: 121 YEDCYSSLEWLGHQASSEPWLQNADPSRVFLSGDSAGGNIVHHVAVKTIKSNIPNLTIKG 180
           YEDCYS+LEWLGHQASSEPWLQ AD S VFLSGDSAGGNIVH+VA+KTIKSNI N+TIKG
Sbjct: 121 YEDCYSALEWLGHQASSEPWLQQADLSHVFLSGDSAGGNIVHNVAIKTIKSNISNMTIKG 180

Query: 181 LLLIHPYFGSKKRTEKEKEEGAAGLVEMNDMFWRLSIPEGRDRDYFGCNYENAQVTKEEW 240
           LLLIHPYFGS++RTEKEK EGA G VEMNDMFWRLSIPEGR+RDYFGCN+ENAQVT+EEW
Sbjct: 181 LLLIHPYFGSEERTEKEKAEGAEGHVEMNDMFWRLSIPEGRNRDYFGCNFENAQVTEEEW 240

Query: 241 SQFPNVVVFVAGLDFLKERGELYAKFLQQMKVKEVKLVEAADESHVFHVFHPESEATRLL 300
           SQFP V VFVAGLDFLKERG LYAKFLQQMKVKEVKL+EAADESHVFHVFHPESEATRLL
Sbjct: 241 SQFPGVAVFVAGLDFLKERGVLYAKFLQQMKVKEVKLLEAADESHVFHVFHPESEATRLL 300

Query: 301 QKQMSQFMKS 310
           QKQMSQFMKS
Sbjct: 301 QKQMSQFMKS 310

BLAST of Spg016878 vs. ExPASy TrEMBL
Match: A0A6J1F8Q9 (probable carboxylesterase 17 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443123 PE=4 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.8e-141
Identity = 247/313 (78.91%), Postives = 279/313 (89.14%), Query Frame = 0

Query: 1   MAMIGEAPGYLQVYSDGSVRRSIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLPDI 60
           MA+IGEAPG+L+VYSDGSVRR IPEVVVST  QSSKNYKFKDV+IDPK+ VTGRLFLP++
Sbjct: 14  MALIGEAPGFLEVYSDGSVRRLIPEVVVSTDNQSSKNYKFKDVVIDPKKPVTGRLFLPNV 73

Query: 61  -ADAPGRLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAA 120
             +   +LP+LVYFHGGGFCIGSTTW+GYH+FLG LA A++ VILS+DYRLAPENRLPAA
Sbjct: 74  TGNHVAKLPILVYFHGGGFCIGSTTWMGYHHFLGGLAVASKCVILSIDYRLAPENRLPAA 133

Query: 121 YEDCYSSLEWLGHQASS-EPWLQNADPSRVFLSGDSAGGNIVHHVAVKTIKSNIPNLTIK 180
           YEDCYSSLEWL HQAS  EPWLQNAD SRVFLSGDSAGGNIVHHVAVKT+K+NI N+T+K
Sbjct: 134 YEDCYSSLEWLAHQASCFEPWLQNADLSRVFLSGDSAGGNIVHHVAVKTMKTNISNMTLK 193

Query: 181 GLLLIHPYFGSKKRTEKEKEEGAAGLVEMNDMFWRLSIPEGRDRDYFGCNYEN--AQVTK 240
           GL+LIHPYFGS+KRTEKEK EG  G VEMNDMFWRLSIP+G +RDYFGCNYEN   +++ 
Sbjct: 194 GLMLIHPYFGSEKRTEKEKAEGVEGFVEMNDMFWRLSIPKGSNRDYFGCNYENNTQEMST 253

Query: 241 EEWSQFPNVVVFVAGLDFLKERGELYAKFLQQMKVKEVKLVEAADESHVFHVFHPESEAT 300
           EEWS+FPNVVVFVAGLDFLKERGELY  FLQQ +VK VKL+EA DESHVFHVF PESE+T
Sbjct: 254 EEWSKFPNVVVFVAGLDFLKERGELYVNFLQQKRVKVVKLIEAVDESHVFHVFRPESEST 313

Query: 301 RLLQKQMSQFMKS 310
           RLLQ QM+QFM +
Sbjct: 314 RLLQMQMTQFMNT 326

BLAST of Spg016878 vs. ExPASy TrEMBL
Match: A0A6J1FDI9 (probable carboxylesterase 17 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443123 PE=4 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.8e-141
Identity = 247/313 (78.91%), Postives = 279/313 (89.14%), Query Frame = 0

Query: 1   MAMIGEAPGYLQVYSDGSVRRSIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLPDI 60
           MA+IGEAPG+L+VYSDGSVRR IPEVVVST  QSSKNYKFKDV+IDPK+ VTGRLFLP++
Sbjct: 1   MALIGEAPGFLEVYSDGSVRRLIPEVVVSTDNQSSKNYKFKDVVIDPKKPVTGRLFLPNV 60

Query: 61  -ADAPGRLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAA 120
             +   +LP+LVYFHGGGFCIGSTTW+GYH+FLG LA A++ VILS+DYRLAPENRLPAA
Sbjct: 61  TGNHVAKLPILVYFHGGGFCIGSTTWMGYHHFLGGLAVASKCVILSIDYRLAPENRLPAA 120

Query: 121 YEDCYSSLEWLGHQASS-EPWLQNADPSRVFLSGDSAGGNIVHHVAVKTIKSNIPNLTIK 180
           YEDCYSSLEWL HQAS  EPWLQNAD SRVFLSGDSAGGNIVHHVAVKT+K+NI N+T+K
Sbjct: 121 YEDCYSSLEWLAHQASCFEPWLQNADLSRVFLSGDSAGGNIVHHVAVKTMKTNISNMTLK 180

Query: 181 GLLLIHPYFGSKKRTEKEKEEGAAGLVEMNDMFWRLSIPEGRDRDYFGCNYEN--AQVTK 240
           GL+LIHPYFGS+KRTEKEK EG  G VEMNDMFWRLSIP+G +RDYFGCNYEN   +++ 
Sbjct: 181 GLMLIHPYFGSEKRTEKEKAEGVEGFVEMNDMFWRLSIPKGSNRDYFGCNYENNTQEMST 240

Query: 241 EEWSQFPNVVVFVAGLDFLKERGELYAKFLQQMKVKEVKLVEAADESHVFHVFHPESEAT 300
           EEWS+FPNVVVFVAGLDFLKERGELY  FLQQ +VK VKL+EA DESHVFHVF PESE+T
Sbjct: 241 EEWSKFPNVVVFVAGLDFLKERGELYVNFLQQKRVKVVKLIEAVDESHVFHVFRPESEST 300

Query: 301 RLLQKQMSQFMKS 310
           RLLQ QM+QFM +
Sbjct: 301 RLLQMQMTQFMNT 313

BLAST of Spg016878 vs. ExPASy TrEMBL
Match: A0A6J1IMT6 (probable carboxylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111476643 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.3e-139
Identity = 247/313 (78.91%), Postives = 275/313 (87.86%), Query Frame = 0

Query: 1   MAMIGEAPGYLQVYSDGSVRRSIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLPD- 60
           MA+IGEAPG+L+VYSDGSVRR IPEVVVST  QSSKNYKFKDV IDPK+ VTGRLFLP+ 
Sbjct: 13  MALIGEAPGFLEVYSDGSVRRLIPEVVVSTDNQSSKNYKFKDVAIDPKKPVTGRLFLPNV 72

Query: 61  IADAPGRLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAA 120
           I +   +LP+LVYFHGGGFCIGSTTW+GYH+FLG LA A+  VILS+DYRLAPENRLPAA
Sbjct: 73  IGNHVAKLPILVYFHGGGFCIGSTTWMGYHHFLGGLAVASNCVILSIDYRLAPENRLPAA 132

Query: 121 YEDCYSSLEWLGHQASS-EPWLQNADPSRVFLSGDSAGGNIVHHVAVKTIKSNIPNLTIK 180
           YEDCYSSLEWL HQAS  EPWLQNAD SRVFL+GDSAGGNIVHHVA+KT+K+NI NLTIK
Sbjct: 133 YEDCYSSLEWLAHQASCFEPWLQNADLSRVFLAGDSAGGNIVHHVALKTMKTNISNLTIK 192

Query: 181 GLLLIHPYFGSKKRTEKEKEEGAAGLVEMNDMFWRLSIPEGRDRDYFGCNYEN--AQVTK 240
           GL+LIHPYFGS+KRTEKEK EG  G VEMNDMFWRLSIP+G + DYFGCNYEN   +++K
Sbjct: 193 GLMLIHPYFGSEKRTEKEKAEGVEGFVEMNDMFWRLSIPKGSNLDYFGCNYENNTQEMSK 252

Query: 241 EEWSQFPNVVVFVAGLDFLKERGELYAKFLQQMKVKEVKLVEAADESHVFHVFHPESEAT 300
           EEWS+FPNVVVFVAGLDFLKERGELY  FLQQ  VK VKL+EA DESHVFHVF PESE T
Sbjct: 253 EEWSKFPNVVVFVAGLDFLKERGELYVNFLQQKWVKVVKLIEAVDESHVFHVFRPESEPT 312

Query: 301 RLLQKQMSQFMKS 310
           RLLQ QM++FM +
Sbjct: 313 RLLQMQMTEFMNT 325

BLAST of Spg016878 vs. ExPASy TrEMBL
Match: A0A7J9JY63 (Abhydrolase_3 domain-containing protein OS=Gossypium armourianum OX=34283 GN=Goarm_004904 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 4.0e-120
Identity = 207/311 (66.56%), Postives = 255/311 (81.99%), Query Frame = 0

Query: 1   MAMIGEAPGYLQVYSDGSVRRSIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLPDI 60
           M+M+ EAP YLQV+SDGSV+R  PE V  T+++SS  YK KDVIIDP + +TGR+FLPDI
Sbjct: 1   MSMVAEAPNYLQVFSDGSVKRFAPE-VAPTSEESSGGYKSKDVIIDPPKAITGRIFLPDI 60

Query: 61  -ADAPGRLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAA 120
                  LPVLVYFHGGGFCIGSTTW+GYH+FLGD A  + +++LSVDYRLAPE+RLP A
Sbjct: 61  HGPCTSSLPVLVYFHGGGFCIGSTTWLGYHHFLGDFAVVSHSIVLSVDYRLAPEHRLPIA 120

Query: 121 YEDCYSSLEWLGHQASSEPWLQNADPSRVFLSGDSAGGNIVHHVAVKTIKSNIPNLTIKG 180
           Y+DCYSSLEWL  Q S+EPWL+ AD SRVFLSGDSAGGNIVH VA+K ++     + +KG
Sbjct: 121 YDDCYSSLEWLCSQVSNEPWLKQADISRVFLSGDSAGGNIVHQVAIKAMRKETLRVKVKG 180

Query: 181 LLLIHPYFGSKKRTEKEKEEGAAGLVEMNDMFWRLSIPEGRDRDYFGCNYENAQVTKEEW 240
           LLLIH +FGS++RTEKE+ +GA G V MNDMFW+LSIP+G +RDYFGCN+E  +V++ EW
Sbjct: 181 LLLIHTFFGSEERTEKERADGATGYVAMNDMFWKLSIPDGSNRDYFGCNFEKQEVSEAEW 240

Query: 241 SQFPNVVVFVAGLDFLKERGELYAKFLQQMKVKEVKLVEAADESHVFHVFHPESEATRLL 300
            +FP V V+VAGLDFLKERG +YAKFLQ+ +VK VKLVE  ++SHVFHVFHP+SEAT  L
Sbjct: 241 REFPAVTVYVAGLDFLKERGVMYAKFLQRKEVKSVKLVETENKSHVFHVFHPKSEATCSL 300

Query: 301 QKQMSQFMKSN 311
           Q+QMS+FMK+N
Sbjct: 301 QRQMSEFMKNN 310

BLAST of Spg016878 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 201.8 bits (512), Expect = 7.9e-52
Identity = 121/305 (39.67%), Postives = 169/305 (55.41%), Query Frame = 0

Query: 3   MIGEAPGYLQVYSDGSVRR-SIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLPDIA 62
           ++ E  G ++V++DG V R  I  +V  T   SSK   F    I        R+++PD A
Sbjct: 28  VVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKATAFD---IKLSNDTWTRVYIPDAA 87

Query: 63  DAPG--RLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAA 122
            A     LP+LVYFHGGGFC+GS  W  YH+FL  LA  AR VI+SV+YRLAPE+RLPAA
Sbjct: 88  AASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHRLPAA 147

Query: 123 YEDCYSSLEWLGHQASS-----EPWLQNADPSRVFLSGDSAGGNIVHHVAVKTIKSN--I 182
           Y+D  + + WL  Q  S       WL   + S VFL+GDSAG NI + VAV+ + S    
Sbjct: 148 YDDGVNVVSWLVKQQISTGGGYPSWLSKCNLSNVFLAGDSAGANIAYQVAVRIMASGKYA 207

Query: 183 PNLTIKGLLLIHPYFGSKKRTEKEKEE----GAAGLVEMNDMFWRLSIPEGRDRDYFGCN 242
             L +KG++LIHP+FG + RT  EK++     +A  +  +D +WRL++P G  RD+  CN
Sbjct: 208 NTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRGASRDHPWCN 267

Query: 243 YENAQVTKEEWSQFPNVVVFVAGLDFLKERGELYAKFLQQMKVKEVKLVEAADESHVFHV 294
                +     ++ P  +VF+A  D LKER     K ++    K V+ +      H FH+
Sbjct: 268 ----PLMSSAGAKLPTTMVFMAEFDILKERNLEMCKVMRS-HGKRVEGIVHGGVGHAFHI 324

BLAST of Spg016878 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 195.7 bits (496), Expect = 5.6e-50
Identity = 122/328 (37.20%), Postives = 184/328 (56.10%), Query Frame = 0

Query: 1   MAMIGEAP-------GYLQVYSDGSVRR--SIPEVVVSTAKQSSKNYKFKDVIIDPKRRV 60
           M  +GE P       G LQ+ S+G+V R  SI  +      ++++   FKD I      +
Sbjct: 1   MGSLGEEPQVAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNL 60

Query: 61  TGRLFLPDIADAPGRLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLA 120
             RL+ P  A     LPV+V+FHGGGFC GS +W  +HNF   LA++   +++S DYRLA
Sbjct: 61  HLRLYKPISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLA 120

Query: 121 PENRLPAAYEDCYSSLEWLGHQASSE---PWLQ---NADPSRVFLSGDSAGGNIVHHVAV 180
           PE+RLPAA+ED  + L WL  QA S+    W +   + D  RVF+ GDS+GGNI H +AV
Sbjct: 121 PEHRLPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAV 180

Query: 181 KTIKSNI--PNLTIKGLLLIHPYFGSKKRTEKEKEEGAAGL-VEMNDMFWRLSIPEGRDR 240
           +    +I    + ++G +L+ P+FG ++RT  E     A L +++ D FWRLS+P G  R
Sbjct: 181 RFGSGSIELTPVRVRGYVLMGPFFGGEERTNSENGPSEALLSLDLLDKFWRLSLPNGATR 240

Query: 241 DYFGCN-YENAQVTKEEWSQFPNVVVFVAGLDFLKERGELYAKFLQQMKVKEVKLVEAAD 300
           D+   N +     T E  S  P ++V V G + L++R + YA  L++M  K V  +E  +
Sbjct: 241 DHHMANPFGPTSPTLESISLEP-MLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFEN 300

Query: 301 ESHVFHVFHPESEATRLLQKQMSQFMKS 310
           + H F+  +P SEA   + + +  FM +
Sbjct: 301 KEHGFYSNYPSSEAAEQVLRIIGDFMNN 327

BLAST of Spg016878 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 195.7 bits (496), Expect = 5.6e-50
Identity = 122/328 (37.20%), Postives = 184/328 (56.10%), Query Frame = 0

Query: 1   MAMIGEAP-------GYLQVYSDGSVRR--SIPEVVVSTAKQSSKNYKFKDVIIDPKRRV 60
           M  +GE P       G LQ+ S+G+V R  SI  +      ++++   FKD I      +
Sbjct: 1   MGSLGEEPQVAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNL 60

Query: 61  TGRLFLPDIADAPGRLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLA 120
             RL+ P  A     LPV+V+FHGGGFC GS +W  +HNF   LA++   +++S DYRLA
Sbjct: 61  HLRLYKPISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLA 120

Query: 121 PENRLPAAYEDCYSSLEWLGHQASSE---PWLQ---NADPSRVFLSGDSAGGNIVHHVAV 180
           PE+RLPAA+ED  + L WL  QA S+    W +   + D  RVF+ GDS+GGNI H +AV
Sbjct: 121 PEHRLPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAV 180

Query: 181 KTIKSNI--PNLTIKGLLLIHPYFGSKKRTEKEKEEGAAGL-VEMNDMFWRLSIPEGRDR 240
           +    +I    + ++G +L+ P+FG ++RT  E     A L +++ D FWRLS+P G  R
Sbjct: 181 RFGSGSIELTPVRVRGYVLMGPFFGGEERTNSENGPSEALLSLDLLDKFWRLSLPNGATR 240

Query: 241 DYFGCN-YENAQVTKEEWSQFPNVVVFVAGLDFLKERGELYAKFLQQMKVKEVKLVEAAD 300
           D+   N +     T E  S  P ++V V G + L++R + YA  L++M  K V  +E  +
Sbjct: 241 DHHMANPFGPTSPTLESISLEP-MLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFEN 300

Query: 301 ESHVFHVFHPESEATRLLQKQMSQFMKS 310
           + H F+  +P SEA   + + +  FM +
Sbjct: 301 KEHGFYSNYPSSEAAEQVLRIIGDFMNN 327

BLAST of Spg016878 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 187.2 bits (474), Expect = 2.0e-47
Identity = 98/261 (37.55%), Postives = 146/261 (55.94%), Query Frame = 0

Query: 3   MIGEAPGYLQVYSDGSVRRSIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLP--DI 62
           ++ E  G ++VY DG V RS     V  +          DV+ID    V  RL++P    
Sbjct: 23  VVDEVEGLIKVYKDGHVERSQLLPCVDPSLPLELGVTCSDVVIDKLTNVWARLYVPMTTT 82

Query: 63  ADAPGRLPVLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAAY 122
             +  +LP++VYFHGGGFC+GS +W+ YH FL  L+A +R +++SV+YRLAPEN LPAAY
Sbjct: 83  KSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLPAAY 142

Query: 123 EDCYSSLEWLGHQASSEPWLQNADPSRVFLSGDSAGGNIVHHVAVKTIKSNIPNLTIKGL 182
           ED  +++ WL    +   W +  D  R+FL+GDSAGGNI   VA +        L I+G 
Sbjct: 143 EDGVNAILWLNKARNDNLWAKQCDFGRIFLAGDSAGGNIAQQVAARLASPEDLALKIEGT 202

Query: 183 LLIHPYFGSKKRTEKEKEEG----AAGLVEMNDMFWRLSIPEGRDRDYFGCNYENAQVTK 242
           +LI P++  ++RTE E+  G    A   +  +D +WR+S+P G +R++  C      +  
Sbjct: 203 ILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANREHPYCKPVKMIIKS 262

Query: 243 EEWSQFPNVVVFVAGLDFLKE 258
              S     +V VA +D L +
Sbjct: 263 ---STVTRTLVCVAEMDLLMD 280

BLAST of Spg016878 vs. TAIR 10
Match: AT1G19190.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 172.6 bits (436), Expect = 5.1e-43
Identity = 102/320 (31.87%), Postives = 166/320 (51.88%), Query Frame = 0

Query: 12  QVYSDGSVRRSIPEVVVSTAKQSSKNYKFKDVIIDPKRRVTGRLFLPD---IADAPGRLP 71
           +++ +G + R +PE  V  +         KD +  P++ ++ R++LP          ++P
Sbjct: 14  RIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRIYLPQNSVYETGEKKIP 73

Query: 72  VLVYFHGGGFCIGSTTWIGYHNFLGDLAAAARTVILSVDYRLAPENRLPAAYEDCYSSLE 131
           +LVYFHGGGF + +     YH FL    +A   + +SV+YR APE+ +P  YED + +++
Sbjct: 74  LLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPIPTLYEDSWDAIQ 133

Query: 132 WLG---HQASSEPWL-QNADPSRVFLSGDSAGGNIVHHVAVKTIKSNIP--NLTIKGLLL 191
           W+     ++  E WL ++AD S+VFL+GDSAG NI HH+A++  K  +P  N  I G++L
Sbjct: 134 WIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPPENFKISGMIL 193

Query: 192 IHPYFGSKKRTEKEKEEGAAGLVEMNDMFWRLSIPE-------------GRDRDYFGCNY 251
            HPYF SK   E+ + E     +   +  WR++ P+             G D    GC  
Sbjct: 194 FHPYFLSKALIEEMEVEA----MRYYERLWRIASPDSGNGVEDPWINVVGSDLTGLGCR- 253

Query: 252 ENAQVTKEEWSQFPNVVVFVAGLDFLKERGELYAKFLQQMK-VKEVKLVEAADESHVFHV 309
                          V+V VAG D L   G  Y   L++   + +VK++E  +E HVFH+
Sbjct: 254 --------------RVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHL 313