Spg016618 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg016618
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionheat stress transcription factor B-4-like
Locationscaffold9: 45662049 .. 45663522 (+)
RNA-Seq ExpressionSpg016618
SyntenySpg016618
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCTAATGCTTGATAATTGCGAAGGCGTTTTGCTTTCCTTAGACTCTCACAAGGCAATCCCAGCTCCATTTCTCACTAAAACCTATCAGCTCGTCGACGATCCCTCCACTGACCATATTGTCTCATGGGGCGAAGATGACACTACCTTCGTCGTTTGGCGTCCTCCTGAATTCGCTAGAGATCTACTTCCTAACTATTTCAAGCACAATAATTTCTCCAGCTTCGTCCGCCAGCTCAACACCTATGTAAGAACATCTCATCCATCGAACAAAAACAAATAAAGATCGTCTTTTTTTCTCTAAAATCTAACCCCTTTCTGTGTCTTCTTTTTTGTAATTTTCTTTAGGGTTTTAGAAAAATTGTGCCGGACAGATGGGAATTTGCGAACGAGTTCTTCAGAAAAGGAGAGAAACATTTATTATGTGAGATCCATAGACGTAAAACCGCTCAACCGCAAGTCGCCGTCAACCAACACCACCAAACTCATTCTCCACTTGGTGTCAACAATCCGAGTTTCTACCACTTCCCCAGCCGAATCAGTATCTCCCCGTCCGACTCCGACGACCAACCCAATTGGTGTGACTCACCGCCGCTCTCTTCCCCTGGAGGCTGCGGCGGCGCCAACAATTACAGCAGCTCCGTCAGCGCCCTCTCGGAGGACAACGAGCGCCTTCGCCGGAGCAACAACATGCTGATGTCGGAATTAGCCCACATGAAAAAACTCTACAACGACATCATCTACTTCGTTCAAAACCATGTGAAGCCCGTGGCACCAAGCAATTCATACCAATACTCAACATCTCTACTTTCCGAGGCTTTTCCGGTGGTGCAACGGCCGCCGAATCATCACCTTCTCGGGTACAATCCGACGAACGCCAAACAAGTTTCCGGGCAGACCCATTTCGTGACCGGAAATCCAAATAACAACGGTTCGTCGAAAAGCTTCGTGACGATTCTAGAAGAACCGAAAACGAAGCTTTTTGGAGTGCCGATTCAATCGAAGAAACGGCTGCACCCCGAGTATGGGTCTAATAATCTTGGCAAAGAGAACAACAAGGCTCGATTGGTGTTGGAAAAAGACGATTTAGGGCTCAATCTCATGCCCCCTTCCGCTTGTTAGTTCAACTTCTTCTCCTAATCCTCAAATTCCTTTAATTTACAGGAAAAAGAAAATTAGAGAGCAAAAAAAAAAAAAGTTATGATCCTTGTTTTATTATTATTATTTTTTTTGGGAATTTTTGTTAGTCTCTCTTACAAGTCTGAGAAGGGATCCTTTTTCAGTGCTGCATTGATGATAATTGAATTAGAAAATGTGTGCGTGCGTATGGTTGTTTGTGATAGTAGTGTAAGTTTCTATGTATATAACCCTCAGTGACGGTATTGGCTTCTCTTTTCCCCATGACCATTATTGTTGCACTGGACTCTGGACTCTCCAATCTCGTCTCTCTCTCTCTGCTTCAAGAGAAGAAG

mRNA sequence

ATGGCTCTAATGCTTGATAATTGCGAAGGCGTTTTGCTTTCCTTAGACTCTCACAAGGCAATCCCAGCTCCATTTCTCACTAAAACCTATCAGCTCGTCGACGATCCCTCCACTGACCATATTGTCTCATGGGGCGAAGATGACACTACCTTCGTCGTTTGGCGTCCTCCTGAATTCGCTAGAGATCTACTTCCTAACTATTTCAAGCACAATAATTTCTCCAGCTTCGTCCGCCAGCTCAACACCTATGGTTTTAGAAAAATTGTGCCGGACAGATGGGAATTTGCGAACGAGTTCTTCAGAAAAGGAGAGAAACATTTATTATGTGAGATCCATAGACGTAAAACCGCTCAACCGCAAGTCGCCGTCAACCAACACCACCAAACTCATTCTCCACTTGGTGTCAACAATCCGAGTTTCTACCACTTCCCCAGCCGAATCAGTATCTCCCCGTCCGACTCCGACGACCAACCCAATTGGTGTGACTCACCGCCGCTCTCTTCCCCTGGAGGCTGCGGCGGCGCCAACAATTACAGCAGCTCCGTCAGCGCCCTCTCGGAGGACAACGAGCGCCTTCGCCGGAGCAACAACATGCTGATGTCGGAATTAGCCCACATGAAAAAACTCTACAACGACATCATCTACTTCGTTCAAAACCATGTGAAGCCCGTGGCACCAAGCAATTCATACCAATACTCAACATCTCTACTTTCCGAGGCTTTTCCGGTGGTGCAACGGCCGCCGAATCATCACCTTCTCGGGTACAATCCGACGAACGCCAAACAAGTTTCCGGGCAGACCCATTTCGTGACCGGAAATCCAAATAACAACGGTTCGTCGAAAAGCTTCGTGACGATTCTAGAAGAACCGAAAACGAAGCTTTTTGGAGTGCCGATTCAATCGAAGAAACGGCTGCACCCCGAGTATGGGTCTAATAATCTTGGCAAAGAGAACAACAAGGCTCGATTGGTGTTGGAAAAAGACGATTTAGGGCTCAATCTCATGCCCCCTTCCGCTTGTTAG

Coding sequence (CDS)

ATGGCTCTAATGCTTGATAATTGCGAAGGCGTTTTGCTTTCCTTAGACTCTCACAAGGCAATCCCAGCTCCATTTCTCACTAAAACCTATCAGCTCGTCGACGATCCCTCCACTGACCATATTGTCTCATGGGGCGAAGATGACACTACCTTCGTCGTTTGGCGTCCTCCTGAATTCGCTAGAGATCTACTTCCTAACTATTTCAAGCACAATAATTTCTCCAGCTTCGTCCGCCAGCTCAACACCTATGGTTTTAGAAAAATTGTGCCGGACAGATGGGAATTTGCGAACGAGTTCTTCAGAAAAGGAGAGAAACATTTATTATGTGAGATCCATAGACGTAAAACCGCTCAACCGCAAGTCGCCGTCAACCAACACCACCAAACTCATTCTCCACTTGGTGTCAACAATCCGAGTTTCTACCACTTCCCCAGCCGAATCAGTATCTCCCCGTCCGACTCCGACGACCAACCCAATTGGTGTGACTCACCGCCGCTCTCTTCCCCTGGAGGCTGCGGCGGCGCCAACAATTACAGCAGCTCCGTCAGCGCCCTCTCGGAGGACAACGAGCGCCTTCGCCGGAGCAACAACATGCTGATGTCGGAATTAGCCCACATGAAAAAACTCTACAACGACATCATCTACTTCGTTCAAAACCATGTGAAGCCCGTGGCACCAAGCAATTCATACCAATACTCAACATCTCTACTTTCCGAGGCTTTTCCGGTGGTGCAACGGCCGCCGAATCATCACCTTCTCGGGTACAATCCGACGAACGCCAAACAAGTTTCCGGGCAGACCCATTTCGTGACCGGAAATCCAAATAACAACGGTTCGTCGAAAAGCTTCGTGACGATTCTAGAAGAACCGAAAACGAAGCTTTTTGGAGTGCCGATTCAATCGAAGAAACGGCTGCACCCCGAGTATGGGTCTAATAATCTTGGCAAAGAGAACAACAAGGCTCGATTGGTGTTGGAAAAAGACGATTTAGGGCTCAATCTCATGCCCCCTTCCGCTTGTTAG

Protein sequence

MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVAVNQHHQTHSPLGVNNPSFYHFPSRISISPSDSDDQPNWCDSPPLSSPGGCGGANNYSSSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSEAFPVVQRPPNHHLLGYNPTNAKQVSGQTHFVTGNPNNNGSSKSFVTILEEPKTKLFGVPIQSKKRLHPEYGSNNLGKENNKARLVLEKDDLGLNLMPPSAC
Homology
BLAST of Spg016618 vs. NCBI nr
Match: XP_022940239.1 (heat stress transcription factor B-4-like [Cucurbita moschata] >XP_023525267.1 heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 620.9 bits (1600), Expect = 6.4e-174
Identity = 304/341 (89.15%), Postives = 321/341 (94.13%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAVNQHHQTHSPLGVNNPSFYHFPSRISISPSDSDDQPNWCDSPPLSSPGGCGGANNYSS 180
           V VNQHHQ++SPLG+ N  FYH+P+R+SISPSDSDD PNWCDSPPLSS       NN+++
Sbjct: 121 VTVNQHHQSYSPLGL-NAGFYHYPARVSISPSDSDDNPNWCDSPPLSSN---NNNNNFNN 180

Query: 181 SVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSEA 240
           SV+ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLS+ 
Sbjct: 181 SVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSDG 240

Query: 241 FPVVQRPPNHHLLGYNPTNAKQVSGQTHFVTGNP-NNNGSSKSFVTILEEPKTKLFGVPI 300
           FPV QR PNHHLLGY+PTNAKQVSGQTHFV G P NNNGSSKSFVTI+EEPKTKLFGV +
Sbjct: 241 FPVGQRQPNHHLLGYHPTNAKQVSGQTHFVAGTPNNNNGSSKSFVTIVEEPKTKLFGVSL 300

Query: 301 QSKKRLHPEYGSNNLGKENNKARLVLEKDDLGLNLMPPSAC 341
           QSKKR+HPEYGSNN+ KENNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 QSKKRVHPEYGSNNIVKENNKARLVLEKDDLGLNLMPPSAC 337

BLAST of Spg016618 vs. NCBI nr
Match: KAG6608467.1 (Heat stress transcription factor B-4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 620.9 bits (1600), Expect = 6.4e-174
Identity = 304/342 (88.89%), Postives = 321/342 (93.86%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAVNQHHQTHSPLGVNNPSFYHFPSRISISPSDSDDQPNWCDSPPLSS-PGGCGGANNYS 180
           V VNQHHQ++SPLG+ N  FYH+P+R+SISPSDSDD PNWCDSPPLSS        NN++
Sbjct: 121 VTVNQHHQSYSPLGL-NAGFYHYPARVSISPSDSDDNPNWCDSPPLSSNNNNSNNNNNFN 180

Query: 181 SSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSE 240
           +SV+ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLS+
Sbjct: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSD 240

Query: 241 AFPVVQRPPNHHLLGYNPTNAKQVSGQTHFVTGNP-NNNGSSKSFVTILEEPKTKLFGVP 300
            FPV QR PNHHLLGY+PTNAKQVSGQTHFV G P NNNGSSKSFVTI+EEPKTKLFGV 
Sbjct: 241 GFPVGQRQPNHHLLGYHPTNAKQVSGQTHFVAGTPNNNNGSSKSFVTIVEEPKTKLFGVS 300

Query: 301 IQSKKRLHPEYGSNNLGKENNKARLVLEKDDLGLNLMPPSAC 341
           +QSKKR+HPEYGSNN+ KENNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 LQSKKRVHPEYGSNNIVKENNKARLVLEKDDLGLNLMPPSAC 341

BLAST of Spg016618 vs. NCBI nr
Match: XP_022982267.1 (heat stress transcription factor B-4-like [Cucurbita maxima])

HSP 1 Score: 617.1 bits (1590), Expect = 9.2e-173
Identity = 303/343 (88.34%), Postives = 320/343 (93.29%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAVNQHHQTHSPLGVNNPSFYHFPSRISISPSDSDDQPNWCDSPPLSSPGGCGGANNYSS 180
           V VNQHHQ++SPLG+ N  FYH+P+R+SISPSDSDD PNWCDSPPLSS       N +++
Sbjct: 121 VTVNQHHQSYSPLGL-NAGFYHYPARVSISPSDSDDNPNWCDSPPLSSNN--NNNNVFNN 180

Query: 181 SVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSEA 240
           SV+ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLS+ 
Sbjct: 181 SVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSDG 240

Query: 241 FPVVQRPPNHHLLGYNPTNAKQVSGQTHFVTGNP---NNNGSSKSFVTILEEPKTKLFGV 300
           FPV QR PNHHLLGY+PTNAKQVSGQTHFV G P   NNNGSSKSFVTI+EEPKTKLFGV
Sbjct: 241 FPVGQRQPNHHLLGYHPTNAKQVSGQTHFVGGTPNNNNNNGSSKSFVTIVEEPKTKLFGV 300

Query: 301 PIQSKKRLHPEYGSNNLGKENNKARLVLEKDDLGLNLMPPSAC 341
            +QSKKR+HPEYGSNN+ KENNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 SLQSKKRVHPEYGSNNIAKENNKARLVLEKDDLGLNLMPPSAC 340

BLAST of Spg016618 vs. NCBI nr
Match: XP_038897232.1 (heat stress transcription factor B-4-like [Benincasa hispida])

HSP 1 Score: 568.5 bits (1464), Expect = 3.8e-158
Identity = 295/362 (81.49%), Postives = 305/362 (84.25%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAVNQHHQTHSPLGVNNPSFYHFPSRISISPSDSDDQPNWCDSPPLSSPGGCGGANNYSS 180
           V VNQHHQ+HSPLG+NNP FYHFP R+SISPSDSDDQ NWCDSP LSSP      NN ++
Sbjct: 121 VTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPSLSSP---APHNNNNN 180

Query: 181 SVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS-TSLLSE 240
           SV+ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS TSLLS+
Sbjct: 181 SVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSLLSD 240

Query: 241 AFPVV-QRPPNHHLLGYNPTNAKQVSGQTHFVTGNP----------------NNNGSSKS 300
            FPVV QRPPNHH   Y+         Q H VTG                  NNN  +KS
Sbjct: 241 RFPVVQQRPPNHHHHHYHQ------QSQIHLVTGTTPTNNNNNHNNNNNIIINNNSGTKS 300

Query: 301 FVTILEE--PKTKLFGVPIQSKKRLHPEYGSNNLGKE--NNKARLVLEKDDLGLNLMPPS 341
           FVTILEE   KTKLFGV IQSKKRLHPEYGSNN+GKE  NNKARLVLE DDLGLNLMPPS
Sbjct: 301 FVTILEEQPTKTKLFGVAIQSKKRLHPEYGSNNIGKENNNNKARLVLENDDLGLNLMPPS 353

BLAST of Spg016618 vs. NCBI nr
Match: XP_023536624.1 (heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 563.5 bits (1451), Expect = 1.2e-156
Identity = 289/344 (84.01%), Postives = 304/344 (88.37%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALM+DNCEGVL+SLDSHK IPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLDSHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ 120

Query: 121 VAVNQHHQTHSPLGVNNPSFYHFPSRISISPSDSDDQPNWCDSPPLSSPGGCGGANNYSS 180
           +     HQ+ SPL +NNP FYHF  R SISPSDSDDQ NWCDSPPLSS  G    NN ++
Sbjct: 121 L-----HQSLSPLALNNPGFYHFHGRGSISPSDSDDQNNWCDSPPLSS-SGTNNNNNNNN 180

Query: 181 SVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSEA 240
           SV+ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST      
Sbjct: 181 SVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST------ 240

Query: 241 FPVVQRPPNHHLLGY-NPTNAKQVSGQTHFVTGNPNNNG-SSKSFVTILEE-PKTKLFGV 300
           FPVVQR PNHHL+GY NPTNAKQVSGQTH VTG PNNN  ++KSFV I+EE  KTKLFGV
Sbjct: 241 FPVVQRQPNHHLVGYNNPTNAKQVSGQTHLVTGIPNNNNKNNKSFVRIVEEQQKTKLFGV 300

Query: 301 PIQSKKRLHPEYGSNNLGKE-NNKARLVLEKDDLGLNLMPPSAC 341
            I SKKRLHPEY SNN+GKE NNKAR VLEKDDLGLNLMPPSAC
Sbjct: 301 AIHSKKRLHPEYASNNIGKENNNKARFVLEKDDLGLNLMPPSAC 332

BLAST of Spg016618 vs. ExPASy Swiss-Prot
Match: Q9C635 (Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.6e-90
Identity = 192/363 (52.89%), Postives = 235/363 (64.74%), Query Frame = 0

Query: 1   MALMLDNCEG----------VLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTT 60
           MA+M++N  G           L+     KA+PAPFLTKTYQLVDDP+TDH+VSWG+DDTT
Sbjct: 1   MAMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFF++GEKHLLCE
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCE 120

Query: 121 IHRRKTAQ--PQ--VAVNQHHQTHSPLGVNNPSFYHFPSRISISPSDSDDQPNWCDSPPL 180
           IHRRKT+Q  PQ       HH     +  +  SF+  P     +P   ++   WCD  P 
Sbjct: 121 IHRRKTSQMIPQQHSPFMSHHHAPPQIPFSGGSFFPLPPPRVTTP---EEDHYWCDDSPP 180

Query: 181 SSPGGCGGANNYSSSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAP 240
           S P       + ++ V+ALSEDNERLRRSN +LMSELAHMKKLYNDIIYFVQNHVKPVAP
Sbjct: 181 SRPRVIPQQIDTAAQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVKPVAP 240

Query: 241 SNSYQYSTSLLSEAFPVVQRPPNHHLLGYNPTNAKQVSGQTHFVTGNPNNNGSSKSFVTI 300
           SN+  Y +S L +     Q+PP          NA  ++          ++  +S+S +T+
Sbjct: 241 SNNSSYLSSFLQK--QQQQQPPTLDYYNTATVNATNLNALN-------SSPPTSQSSITV 300

Query: 301 LEEP-----------KTKLFGVPIQSKKRLHPEYGSNNLGKENNKARLVLEKDDLGLNLM 339
           LE+            KTKLFGV + S K+      S++   + +K RLVL++ DL LNLM
Sbjct: 301 LEDDHTNHHDQSNMRKTKLFGVSLPSSKK-----RSHHFSDQTSKTRLVLDQSDLALNLM 346

BLAST of Spg016618 vs. ExPASy Swiss-Prot
Match: Q7XHZ0 (Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4B PE=1 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.2e-71
Identity = 159/323 (49.23%), Postives = 194/323 (60.06%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLD----------SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTT 60
           MA +++ C  +++S++          + K +PAPFLTKTYQLVDDP TDHIVSWGEDDTT
Sbjct: 1   MAFLVERCGEMVVSMEMGPHGGGGAAAGKPVPAPFLTKTYQLVDDPCTDHIVSWGEDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL E
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLLAE 120

Query: 121 IHRRKTAQPQVAVNQHHQTHSPLGVN------NPSFYHFPSRISISPSDSDDQPNWCDSP 180
           IHRRK++QP      H   H    +N       P  YH    I        ++P      
Sbjct: 121 IHRRKSSQPPPPPMPHQPYHHHHHLNPFSLPPPPPAYHHHHLI-------QEEPATTAHC 180

Query: 181 PLSSPGGCGGANNYSSSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPV 240
            ++  GG GG       ++ALSEDN +LRR N++L+SELAHMKKLYNDIIYF+QNHV PV
Sbjct: 181 TVAGDGGEGG-----DFLAALSEDNRQLRRRNSLLLSELAHMKKLYNDIIYFLQNHVAPV 240

Query: 241 APSNSYQYSTSLLSEAFPVVQRPPNHHLLGYNPTNAKQVSGQTHFVTGNPNNNGSSKSFV 300
             + +   ST++ +           HHL         ++    H     P    ++    
Sbjct: 241 TTTTTTPSSTAMAA---------AQHHLPAAASCRLMELDSPDHSPPPPPPKTPATDGGD 296

Query: 301 TILEEPKTKLFGVPIQS-KKRLH 307
           T+      KLFGV +   KKR H
Sbjct: 301 TV------KLFGVSLHGRKKRAH 296

BLAST of Spg016618 vs. ExPASy Swiss-Prot
Match: Q67U94 (Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4C PE=2 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 5.8e-69
Identity = 175/396 (44.19%), Postives = 216/396 (54.55%), Query Frame = 0

Query: 7   NCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDD-TTFVVWRPPEFARDLLP 66
           +CE    +  + KA+PAPFLTKTYQLVDDP+TDHIVSWG+D  +TFVVWRPPEFARD+LP
Sbjct: 9   DCEAAAAA--AQKAVPAPFLTKTYQLVDDPATDHIVSWGDDRVSTFVVWRPPEFARDILP 68

Query: 67  NYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA-------- 126
           NYFKHNNFSSFVRQLNTYGFRK+VP+RWEFANEFFRKGEK LL EIHRRKT+        
Sbjct: 69  NYFKHNNFSSFVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLTEIHRRKTSSASTASPS 128

Query: 127 --------------QPQVAVNQHHQTHSPLGVNNPSFYH-----FPSRISISPSDSDDQP 186
                          P VA  QHH  H+ +G +     H     FP            QP
Sbjct: 129 PPPFFAPPHFPLFHHPGVAAAQHH--HAFVGDDGVVAAHGIGMPFP------------QP 188

Query: 187 NW-----------------CDSPPLSSPGGCGGANNYSSSVSALSEDNERLRRSNNMLMS 246
           +W                   SP  +  GG G A    ++ + L E+NERLRRSN  L+ 
Sbjct: 189 HWREPNLPVATRLLALGGPAPSPSSAEAGGAGRA----ATAAVLMEENERLRRSNTALLQ 248

Query: 247 ELAHMKKLYNDIIYFVQNHVKPVAPS---NSYQYSTSLLSEAFPVVQRPPNHHLLGYNPT 306
           ELAHM+KLYNDIIYFVQNHV+PVAPS    ++     + +   P      N+   G + T
Sbjct: 249 ELAHMRKLYNDIIYFVQNHVRPVAPSPAAAAFLQGLGMQARKKPAAANVLNNS--GGSTT 308

Query: 307 NAKQVS--------GQTHFVTGNPNNNGSSKSFVTILEEPKTKLFGVPIQ-------SKK 340
           ++  ++         Q   + G  +   +  S       P TKLFGV +        SK+
Sbjct: 309 SSSSLTIAEEPSPPPQQQHLAGEKSGGEAGNSSAARSSAP-TKLFGVHLSAAPCGAGSKR 368

BLAST of Spg016618 vs. ExPASy Swiss-Prot
Match: Q10KX8 (Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4D PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.9e-67
Identity = 142/242 (58.68%), Postives = 172/242 (71.07%), Query Frame = 0

Query: 1   MALMLDNCEG-VLLSLD-SH----------KAIPAPFLTKTYQLVDDPSTDHIVSWGEDD 60
           MA +++ C G +++S++ SH           A PAPFL+KTYQLVDDPSTD +VSWGED+
Sbjct: 1   MAFLVERCGGEMVVSMERSHGRSTTTAAAVTAAPAPFLSKTYQLVDDPSTDDVVSWGEDE 60

Query: 61  TTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLL 120
            TFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL
Sbjct: 61  ATFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLL 120

Query: 121 CEIHRRKT---AQPQVAVNQHHQTHSPLGV-NNPSFYHFPSRISISPSDSDDQPNWCDSP 180
            EIHRRK+   +QPQ         H PL + + P+    P   + + +    Q  +C SP
Sbjct: 121 SEIHRRKSSSCSQPQPPPPFPMHQHYPLSLFSPPTTPRSPPVGAAAAAAYHFQEEYCSSP 180

Query: 181 PLSSPGGCGGANNYSSSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPV 227
              + GG          ++ALSEDN +LRR N++L+SELAHM+KLYNDIIYF+QNHV+PV
Sbjct: 181 ADYAGGG-------GDLLAALSEDNRQLRRRNSLLLSELAHMRKLYNDIIYFLQNHVEPV 235

BLAST of Spg016618 vs. ExPASy Swiss-Prot
Match: Q6Z9R8 (Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4A PE=3 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.9e-60
Identity = 148/335 (44.18%), Postives = 189/335 (56.42%), Query Frame = 0

Query: 17  SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDD-----TTFVVWRPPEFARDLLPNYFKHN 76
           S   +PAPFLTKTYQLVDDP+TDH+VSW +DD     ++FVVWRPPEFARD+LPNYFKH+
Sbjct: 18  SASVVPAPFLTKTYQLVDDPATDHVVSWEDDDGGESASSFVVWRPPEFARDILPNYFKHS 77

Query: 77  NFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVAVNQHHQTHS 136
           NFSSFVRQLNTYGFRK+VP+RWEFANEFFRKGEK LLCEIHRRK+A           T  
Sbjct: 78  NFSSFVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLCEIHRRKSAA---------ATWP 137

Query: 137 PLGVNNPSFY---HFPSRISISPSD------------SDDQPNWCDSP------------ 196
           P     P F+   HF +       D            + ++ +W +S             
Sbjct: 138 PFPPPPPPFFAPRHFAAGAFFRHGDGMLHGRLGALVTTTERRHWFESAALPVAPSSRLLS 197

Query: 197 ---PLSSPGGCGGANNYSSSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHV 256
              P+ +P     A   +    AL ++N RL R N  L+ ELAHM+KLY+DIIYFVQNHV
Sbjct: 198 QLGPVIAPARRAAA---TPEEEALMQENHRLLRGNAALVQELAHMRKLYSDIIYFVQNHV 257

Query: 257 KPVAPSNSYQYSTSLLSEAFPVVQRPPNHHLLGYNPTNAKQVSGQTHFVTGNPNNNGSSK 316
           +PVAPS +   +   L      V RPP        P   K  + +   V G    + +S 
Sbjct: 258 RPVAPSPAAAAALHGLG-----VLRPP--------PAGGKAPASE---VRGASGRSATSS 317

BLAST of Spg016618 vs. ExPASy TrEMBL
Match: A0A6J1FJH2 (heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC111445920 PE=3 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.1e-174
Identity = 304/341 (89.15%), Postives = 321/341 (94.13%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAVNQHHQTHSPLGVNNPSFYHFPSRISISPSDSDDQPNWCDSPPLSSPGGCGGANNYSS 180
           V VNQHHQ++SPLG+ N  FYH+P+R+SISPSDSDD PNWCDSPPLSS       NN+++
Sbjct: 121 VTVNQHHQSYSPLGL-NAGFYHYPARVSISPSDSDDNPNWCDSPPLSSN---NNNNNFNN 180

Query: 181 SVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSEA 240
           SV+ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLS+ 
Sbjct: 181 SVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSDG 240

Query: 241 FPVVQRPPNHHLLGYNPTNAKQVSGQTHFVTGNP-NNNGSSKSFVTILEEPKTKLFGVPI 300
           FPV QR PNHHLLGY+PTNAKQVSGQTHFV G P NNNGSSKSFVTI+EEPKTKLFGV +
Sbjct: 241 FPVGQRQPNHHLLGYHPTNAKQVSGQTHFVAGTPNNNNGSSKSFVTIVEEPKTKLFGVSL 300

Query: 301 QSKKRLHPEYGSNNLGKENNKARLVLEKDDLGLNLMPPSAC 341
           QSKKR+HPEYGSNN+ KENNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 QSKKRVHPEYGSNNIVKENNKARLVLEKDDLGLNLMPPSAC 337

BLAST of Spg016618 vs. ExPASy TrEMBL
Match: A0A6J1IW73 (heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC111481146 PE=3 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 4.5e-173
Identity = 303/343 (88.34%), Postives = 320/343 (93.29%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAVNQHHQTHSPLGVNNPSFYHFPSRISISPSDSDDQPNWCDSPPLSSPGGCGGANNYSS 180
           V VNQHHQ++SPLG+ N  FYH+P+R+SISPSDSDD PNWCDSPPLSS       N +++
Sbjct: 121 VTVNQHHQSYSPLGL-NAGFYHYPARVSISPSDSDDNPNWCDSPPLSSNN--NNNNVFNN 180

Query: 181 SVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSEA 240
           SV+ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLS+ 
Sbjct: 181 SVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSDG 240

Query: 241 FPVVQRPPNHHLLGYNPTNAKQVSGQTHFVTGNP---NNNGSSKSFVTILEEPKTKLFGV 300
           FPV QR PNHHLLGY+PTNAKQVSGQTHFV G P   NNNGSSKSFVTI+EEPKTKLFGV
Sbjct: 241 FPVGQRQPNHHLLGYHPTNAKQVSGQTHFVGGTPNNNNNNGSSKSFVTIVEEPKTKLFGV 300

Query: 301 PIQSKKRLHPEYGSNNLGKENNKARLVLEKDDLGLNLMPPSAC 341
            +QSKKR+HPEYGSNN+ KENNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 SLQSKKRVHPEYGSNNIAKENNKARLVLEKDDLGLNLMPPSAC 340

BLAST of Spg016618 vs. ExPASy TrEMBL
Match: A0A6J1IFM1 (heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC111476507 PE=3 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.9e-157
Identity = 289/345 (83.77%), Postives = 303/345 (87.83%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALM+DNCEGVL+SL+ HK IPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLEPHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQP- 120

Query: 121 VAVNQHHQTHSPLGVNNPSFYHFPSRISISPSDSDDQPNWCDSPPLSSPG-GCGGANNYS 180
               QHHQ+ SPL +NNP FYHF  R SISPSDSDDQ NWCDSPPLSS G      NNYS
Sbjct: 121 ----QHHQSLSPLALNNPGFYHFHGRGSISPSDSDDQNNWCDSPPLSSSGTNNNNNNNYS 180

Query: 181 SSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSE 240
           +SV+ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST     
Sbjct: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST----- 240

Query: 241 AFPVVQRPPNHHLLGY-NPTNAKQVSGQTHFVTGNPNNNG-SSKSFVTILEE-PKTKLFG 300
            FPVVQR PNHHL+GY NPTNAK+VSGQTH VTG PNNN  S+KS V I+EE  KTKLFG
Sbjct: 241 -FPVVQRQPNHHLVGYNNPTNAKRVSGQTHLVTGTPNNNNDSNKSLVRIVEEQQKTKLFG 300

Query: 301 VPIQSKKRLHPEYGSNNLGKE-NNKARLVLEKDDLGLNLMPPSAC 341
           V I SKKRLHPEY SNN+GKE NNKAR VLEKDDLGLNLMPPSAC
Sbjct: 301 VAIHSKKRLHPEYASNNIGKENNNKARFVLEKDDLGLNLMPPSAC 334

BLAST of Spg016618 vs. ExPASy TrEMBL
Match: A0A6J1F8Q8 (heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC111443150 PE=3 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.3e-156
Identity = 288/344 (83.72%), Postives = 303/344 (88.08%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALM+DNCEGVL+SL+SHK IPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQP- 120

Query: 121 VAVNQHHQTHSPLGVNNPSFYHFPSRISISPSDSDDQPNWCDSPPLSSPGGCGGANNYSS 180
               QH Q+ SPL +NNP FYHF +R SISPSDSDDQ NWCDSPPLSS     G NN ++
Sbjct: 121 ----QHQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSS----SGTNNNNN 180

Query: 181 SVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSEA 240
           SVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST      
Sbjct: 181 SVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST------ 240

Query: 241 FPVVQRPPNHHLLGY-NPTNAKQVSGQTHFVTGNP-NNNGSSKSFVTILEE-PKTKLFGV 300
           FPVVQR PNHHL+GY NPTNAKQVSGQTH VT  P NNN ++KSFV I+EE  KTKLFGV
Sbjct: 241 FPVVQRQPNHHLVGYNNPTNAKQVSGQTHLVTATPNNNNNNNKSFVRIVEEQQKTKLFGV 300

Query: 301 PIQSKKRLHPEYGSNNLGKE-NNKARLVLEKDDLGLNLMPPSAC 341
            I SKKRLHPEY SNN+GKE NNKAR VLEKDDLGLNLMPPSAC
Sbjct: 301 AIHSKKRLHPEYASNNIGKENNNKARFVLEKDDLGLNLMPPSAC 329

BLAST of Spg016618 vs. ExPASy TrEMBL
Match: A0A0A0L4W8 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639930 PE=3 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.8e-145
Identity = 278/349 (79.66%), Postives = 296/349 (84.81%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD C+GVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAVNQHHQTHSPLGVNNPSFYHFP-SRISISPSDSDDQPN-WCDSPPLSSPGGCGGANNY 180
           V VNQHHQ HSPL   NP FYHFP +R+SISPSDSDDQ N WCDSP   SP      NN 
Sbjct: 121 VTVNQHHQPHSPL---NPGFYHFPTARLSISPSDSDDQNNYWCDSP---SPN-----NNN 180

Query: 181 SSSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS--TSL 240
           ++SV+ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS  TSL
Sbjct: 181 NNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSL 240

Query: 241 LSEAFPVVQRPPNHHLLGYNPTNAKQVSGQTHFVTGNPNNNGSSKSFVTILEE-----PK 300
           LS+ FPVV++P ++H   ++  + +QVS Q        NN   +KSFVTILEE      K
Sbjct: 241 LSDGFPVVRQPNHYH---HHHHHHQQVSSQIR------NNTVGTKSFVTILEEEQQQQTK 300

Query: 301 TKLFGVPIQSKKRLHPEYGSNNLGKENNKARLVLEKDDLGLNLMPPSAC 341
           TKLFGV IQSKKRLHPEYG++N    NNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 TKLFGVAIQSKKRLHPEYGNSNSNNNNNKARLVLEKDDLGLNLMPPSAC 329

BLAST of Spg016618 vs. TAIR 10
Match: AT1G46264.1 (heat shock transcription factor B4 )

HSP 1 Score: 334.3 bits (856), Expect = 1.1e-91
Identity = 192/363 (52.89%), Postives = 235/363 (64.74%), Query Frame = 0

Query: 1   MALMLDNCEG----------VLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTT 60
           MA+M++N  G           L+     KA+PAPFLTKTYQLVDDP+TDH+VSWG+DDTT
Sbjct: 1   MAMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFF++GEKHLLCE
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCE 120

Query: 121 IHRRKTAQ--PQ--VAVNQHHQTHSPLGVNNPSFYHFPSRISISPSDSDDQPNWCDSPPL 180
           IHRRKT+Q  PQ       HH     +  +  SF+  P     +P   ++   WCD  P 
Sbjct: 121 IHRRKTSQMIPQQHSPFMSHHHAPPQIPFSGGSFFPLPPPRVTTP---EEDHYWCDDSPP 180

Query: 181 SSPGGCGGANNYSSSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAP 240
           S P       + ++ V+ALSEDNERLRRSN +LMSELAHMKKLYNDIIYFVQNHVKPVAP
Sbjct: 181 SRPRVIPQQIDTAAQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVKPVAP 240

Query: 241 SNSYQYSTSLLSEAFPVVQRPPNHHLLGYNPTNAKQVSGQTHFVTGNPNNNGSSKSFVTI 300
           SN+  Y +S L +     Q+PP          NA  ++          ++  +S+S +T+
Sbjct: 241 SNNSSYLSSFLQK--QQQQQPPTLDYYNTATVNATNLNALN-------SSPPTSQSSITV 300

Query: 301 LEEP-----------KTKLFGVPIQSKKRLHPEYGSNNLGKENNKARLVLEKDDLGLNLM 339
           LE+            KTKLFGV + S K+      S++   + +K RLVL++ DL LNLM
Sbjct: 301 LEDDHTNHHDQSNMRKTKLFGVSLPSSKK-----RSHHFSDQTSKTRLVLDQSDLALNLM 346

BLAST of Spg016618 vs. TAIR 10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 186.8 bits (473), Expect = 2.9e-47
Identity = 104/214 (48.60%), Postives = 136/214 (63.55%), Query Frame = 0

Query: 16  DSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSS 75
           DS ++IP PFLTKTYQLV+DP  D ++SW ED TTF+VWRP EFARDLLP YFKHNNFSS
Sbjct: 51  DSQRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSS 110

Query: 76  FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVAVNQHHQTHSPLGV 135
           FVRQLNTYGFRK+VPDRWEF+N+ F++GEK LL +I RRK +QP +A        + +  
Sbjct: 111 FVRQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAA-AAAAAAAAVAA 170

Query: 136 NNPSFYHFPSRISI-SPSDS-DDQPNWCDSPPLSSPGGCGGA--------NNYSSSVSAL 195
           +  +    P    I SPS+S ++Q    +S P ++    GG             ++   L
Sbjct: 171 SAVTVAAVPVVAHIVSPSNSGEEQVISSNSSPAAAAAAIGGVVGGGSLQRTTSCTTAPEL 230

Query: 196 SEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQN 220
            E+NERLR+ N  L  E+  +K LY +I   + N
Sbjct: 231 VEENERLRKDNERLRKEMTKLKGLYANIYTLMAN 263

BLAST of Spg016618 vs. TAIR 10
Match: AT4G36990.1 (heat shock factor 4 )

HSP 1 Score: 183.7 bits (465), Expect = 2.4e-46
Identity = 99/206 (48.06%), Postives = 131/206 (63.59%), Query Frame = 0

Query: 17  SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           + +++PAPFL+KTYQLVDD STD +VSW E+ T FVVW+  EFA+DLLP YFKHNNFSSF
Sbjct: 7   AQRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSF 66

Query: 77  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVAVNQHHQTHSPLGVN 136
           +RQLNTYGFRK VPD+WEFAN++FR+G + LL +I RRK+     A          + V 
Sbjct: 67  IRQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRKSVIASTA-------GKCVVVG 126

Query: 137 NPSFYHFPSRISISPSDSDDQPNWCDSPPLSSPGGCGGANNYSSSVSALSEDNERLRRSN 196
           +PS          +    DD      S   SSPG      +  + V+ LS +NE+L+R N
Sbjct: 127 SPS--------ESNSGGGDDH----GSSSTSSPGSSKNPGSVENMVADLSGENEKLKREN 186

Query: 197 NMLMSELAHMKKLYNDIIYFVQNHVK 223
           N L SELA  KK  ++++ F+  H+K
Sbjct: 187 NNLSSELAAAKKQRDELVTFLTGHLK 193

BLAST of Spg016618 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 173.7 bits (439), Expect = 2.5e-43
Identity = 99/218 (45.41%), Postives = 132/218 (60.55%), Query Frame = 0

Query: 17  SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           S ++IP PFLTKT+ LV+D S D ++SW ED ++F+VW P +FA+DLLP +FKHNNFSSF
Sbjct: 16  SQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSSF 75

Query: 77  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVAVNQHHQTHSPLGVN 136
           VRQLNTYGF+K+VPDRWEF+N+FF++GEK LL EI RRK       +   HQT     V 
Sbjct: 76  VRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRK-------ITTTHQT-----VV 135

Query: 137 NPSFYHFPSRISISPSDSDDQPNWCDSPPLSSPGGCGGANNYSSSVSALS----EDNERL 196
            PS       + +SPS+S +  N  +    SSP         ++    LS    E+NE+L
Sbjct: 136 APSSEQRNQTMVVSPSNSGEDNN-NNQVMSSSPSSWYCHQTKTTGNGGLSVELLEENEKL 195

Query: 197 RRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSY 231
           R  N  L  EL  MK + ++I   + N+V       SY
Sbjct: 196 RSQNIQLNRELTQMKSICDNIYSLMSNYVGSQPTDRSY 220

BLAST of Spg016618 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 159.5 bits (402), Expect = 4.9e-39
Identity = 69/99 (69.70%), Postives = 84/99 (84.85%), Query Frame = 0

Query: 20  AIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQ 79
           ++P PFL+KTY +V+DP+TD IVSW   + +F+VW PPEF+RDLLP YFKHNNFSSFVRQ
Sbjct: 48  SLPPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQ 107

Query: 80  LNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQ 119
           LNTYGFRK+ PDRWEFANE F +G+KHLL +I RRK+ Q
Sbjct: 108 LNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQ 146

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022940239.16.4e-17489.15heat stress transcription factor B-4-like [Cucurbita moschata] >XP_023525267.1 h... [more]
KAG6608467.16.4e-17488.89Heat stress transcription factor B-4, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022982267.19.2e-17388.34heat stress transcription factor B-4-like [Cucurbita maxima][more]
XP_038897232.13.8e-15881.49heat stress transcription factor B-4-like [Benincasa hispida][more]
XP_023536624.11.2e-15684.01heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9C6351.6e-9052.89Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE... [more]
Q7XHZ01.2e-7149.23Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q67U945.8e-6944.19Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q10KX81.9e-6758.68Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q6Z9R82.9e-6044.18Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica O... [more]
Match NameE-valueIdentityDescription
A0A6J1FJH23.1e-17489.15heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IW734.5e-17388.34heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1IFM15.9e-15783.77heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1F8Q81.3e-15683.72heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A0A0L4W81.8e-14579.66HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639930 ... [more]
Match NameE-valueIdentityDescription
AT1G46264.11.1e-9152.89heat shock transcription factor B4 [more]
AT4G11660.12.9e-4748.60winged-helix DNA-binding transcription factor family protein [more]
AT4G36990.12.4e-4648.06heat shock factor 4 [more]
AT5G62020.12.5e-4345.41heat shock transcription factor B2A [more]
AT4G17750.14.9e-3969.70heat shock factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 182..202
NoneNo IPR availablePANTHERPTHR10015:SF382BNAC06G00310D PROTEINcoord: 12..338
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 25..48
score: 47.38
coord: 63..75
score: 78.68
coord: 76..88
score: 84.62
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 21..114
e-value: 5.1E-60
score: 215.4
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 25..114
e-value: 2.6E-32
score: 111.3
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 64..88
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 17..116
e-value: 1.2E-37
score: 130.5
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 12..338
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 22..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg016618.1Spg016618.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding