Spg016532 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg016532
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	isoamylase 3, chloroplastic isoform X1
Location	scaffold9: 42300139 .. 42301354 (-)
RNA-Seq Expression	Spg016532
Synteny	Spg016532

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAAGGGGGAGGCCAATTTCGAGATCCTTGTAATCCGTTCACGTGGCAGACATGAAAAGGCATCCGGTTTCCTTGCAAATAAGCGAAGAAAAAAAACCCGATTTCCAAAAAGCTCCACAAAATCCCATTCTCTAACGAACCTGCATGCAATGATCTTCATCTTCGTCGCTACTTTCCACACTTCCGCGCTACTTCTTTCAGTTCTCTCGGCAATCTGATCGCTTCCACCTTCGAATATGCTCCGCTCTCTATCTCTCCATCACTCCGCTGCTCTTCAGCTTCCTCTCTTCGCGTCATCCTGCACCGGAACTCCTCGTTTCCATCAGCTGCGGTACTTTTCCTCAATCTATTGGCGTTTTTCTGCTTCTTTCAGCGTTTTCTCACTTCGATTCTCAATTCGGAATTGGAGCAGTCTACAATGCCGTCCTATTTGATATGGAGTTCTATTGATTTGGCTGTGTGTGTCGGATTGCGTGTTTCTTTTTTCCTCTTGACTTTCTTTTCCTCATTCGTTTGATTAGATTGAAGCCGCAAGGGAGGCGAGCGGCAGGCGGCGGCAGCATCCGCAGCAGCCAGGAGTTTCTTAGTGAGGTACGATGATTGACGAATCCGCAACTTATGACTTGTTGCTTTCAGCTTGTGATGAATGTCTCGTGTTTGCGAGTGACTTGGCCGAGCTTTCTGCTTTGGTTAGACTAAGGAGGGAAGCGTCAAGCGTGGCTCTGTAAGTGCACTTGGTCGTCGTGGTCAAGATCGTGTGCTGGAGGTTCATACTTATTCCTTATGGACTGGACGTGTTTGTTTTTTTAGTTCCATCATACGTTTTTTTGTAGGAAGAACGAATTGATGTTTGCAAATTAAAAAATCTCTTAATAGTGATTAAGAAAAGTCACCACGGATGGAGAAGAAATAAATATCAATAACAATAGATTTGGGGATGAAAATAAGTTCAAAACCAGCACAAGTATCAAACTGGTAGCCACAGCCCACATCTTTGGGACGTTGGAAAAAAATGTGGGATATCCAACTCTTAATTATTTGCGGAACATCATTGTAGTGAGTTAGTGGTTCTCCAAGAATACTAATTAACTAGTTACTTTATTTTCAGATCATGAAGCTTTGTCGTTACTTCATGGACTACTTTTCTGTGGCAATTGATTTTCCATTATTTGCAATTTATGCCACGATTGATGACAGAAATGGGGACGTGATGTAG

mRNA sequence

ATGCTCCGCTCTCTATCTCTCCATCACTCCGCTGCTCTTCAGCTTCCTCTCTTCGCGTCATCCTGCACCGGAACTCCTCGTTTCCATCAGCTGCGATTGAAGCCGCAAGGGAGGCGAGCGGCAGGCGGCGGCAGCATCCGCAGCAGCCAGGAGTTTCTTAGTGAGACTAAGGAGGGAAGCGTCAAGCGTGGCTCTGTAAGTGCACTTGGTCGTCGTGGTCAAGATCGTGTGCTGGAGATCATGAAGCTTTGTCGTTACTTCATGGACTACTTTTCTGTGGCAATTGATTTTCCATTATTTGCAATTTATGCCACGATTGATGACAGAAATGGGGACGTGATGTAG

Coding sequence (CDS)

ATGCTCCGCTCTCTATCTCTCCATCACTCCGCTGCTCTTCAGCTTCCTCTCTTCGCGTCATCCTGCACCGGAACTCCTCGTTTCCATCAGCTGCGATTGAAGCCGCAAGGGAGGCGAGCGGCAGGCGGCGGCAGCATCCGCAGCAGCCAGGAGTTTCTTAGTGAGACTAAGGAGGGAAGCGTCAAGCGTGGCTCTGTAAGTGCACTTGGTCGTCGTGGTCAAGATCGTGTGCTGGAGATCATGAAGCTTTGTCGTTACTTCATGGACTACTTTTCTGTGGCAATTGATTTTCCATTATTTGCAATTTATGCCACGATTGATGACAGAAATGGGGACGTGATGTAG

Protein sequence

MLRSLSLHHSAALQLPLFASSCTGTPRFHQLRLKPQGRRAAGGGSIRSSQEFLSETKEGSVKRGSVSALGRRGQDRVLEIMKLCRYFMDYFSVAIDFPLFAIYATIDDRNGDVM

Homology

[Close]

BLAST of Spg016532 vs. NCBI nr
Match: XP_022936809.1 (isoamylase 3, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 96.7 bits (239), Expect = 1.4e-16
Identity = 54/79 (68.35%), Postives = 59/79 (74.68%), Query Frame = 0


Query: 1  MLRSLSLHHSAALQLPLFASSCTGTPRFHQLRLKPQGRRAAGGGSIRSSQEFLSETKEGS 60
          M+RS+ LHHSA LQLPLFASSCTG     Q RLKPQG+RA G  S  SSQE  SETK+G 
Sbjct: 1  MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSS-SSSQEVFSETKQGG 60

Query: 61 VKRGSVSALGRRGQDRVLE 80
          VK  +VSA GRR QDRVLE
Sbjct: 61 VKICTVSAFGRRDQDRVLE 78

[Close]

BLAST of Spg016532 vs. NCBI nr
Match: KAG7024560.1 (Isoamylase 3, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 96.7 bits (239), Expect = 1.4e-16
Identity = 54/79 (68.35%), Postives = 59/79 (74.68%), Query Frame = 0


Query: 1  MLRSLSLHHSAALQLPLFASSCTGTPRFHQLRLKPQGRRAAGGGSIRSSQEFLSETKEGS 60
          M+RS+ LHHSA LQLPLFASSCTG     Q RLKPQG+RA G  S  SSQE  SETK+G 
Sbjct: 1  MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSS-SSSQEVFSETKQGG 60

Query: 61 VKRGSVSALGRRGQDRVLE 80
          VK  +VSA GRR QDRVLE
Sbjct: 61 VKICTVSAFGRRDQDRVLE 78

[Close]

BLAST of Spg016532 vs. NCBI nr
Match: KAG6591680.1 (Isoamylase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 96.7 bits (239), Expect = 1.4e-16
Identity = 54/79 (68.35%), Postives = 59/79 (74.68%), Query Frame = 0


Query: 1  MLRSLSLHHSAALQLPLFASSCTGTPRFHQLRLKPQGRRAAGGGSIRSSQEFLSETKEGS 60
          M+RS+ LHHSA LQLPLFASSCTG     Q RLKPQG+RA G  S  SSQE  SETK+G 
Sbjct: 1  MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSS-SSSQEVFSETKQGG 60

Query: 61 VKRGSVSALGRRGQDRVLE 80
          VK  +VSA GRR QDRVLE
Sbjct: 61 VKICTVSAFGRRDQDRVLE 78

[Close]

BLAST of Spg016532 vs. NCBI nr
Match: XP_023535183.1 (isoamylase 3, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 93.2 bits (230), Expect = 1.6e-15
Identity = 52/79 (65.82%), Postives = 57/79 (72.15%), Query Frame = 0


Query: 1  MLRSLSLHHSAALQLPLFASSCTGTPRFHQLRLKPQGRRAAGGGSIRSSQEFLSETKEGS 60
          M+RS+ LHHSA LQLPLFASSCTG     Q RLKPQG+RA G     SSQE  SE K+G 
Sbjct: 1  MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSS---SSQEVFSEAKQGG 60

Query: 61 VKRGSVSALGRRGQDRVLE 80
          VK  +VSA GRR QDRVLE
Sbjct: 61 VKICTVSAFGRRDQDRVLE 76

[Close]

BLAST of Spg016532 vs. NCBI nr
Match: XP_022975928.1 (isoamylase 3, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 91.7 bits (226), Expect = 4.5e-15
Identity = 51/79 (64.56%), Postives = 56/79 (70.89%), Query Frame = 0


Query: 1  MLRSLSLHHSAALQLPLFASSCTGTPRFHQLRLKPQGRRAAGGGSIRSSQEFLSETKEGS 60
          M+RS+ LHHSA LQLPLFASSCTG     Q RLKPQG+R  G     SSQE  SETK+G 
Sbjct: 1  MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRGPGSS---SSQEVFSETKQGG 60

Query: 61 VKRGSVSALGRRGQDRVLE 80
          VK  +VSA GRR QD VLE
Sbjct: 61 VKICTVSAFGRRDQDHVLE 76

[Close]

BLAST of Spg016532 vs. ExPASy TrEMBL
Match: A0A6J1F9D1 (isoamylase 3, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443282 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 6.8e-17
Identity = 54/79 (68.35%), Postives = 59/79 (74.68%), Query Frame = 0


Query: 1  MLRSLSLHHSAALQLPLFASSCTGTPRFHQLRLKPQGRRAAGGGSIRSSQEFLSETKEGS 60
          M+RS+ LHHSA LQLPLFASSCTG     Q RLKPQG+RA G  S  SSQE  SETK+G 
Sbjct: 1  MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSS-SSSQEVFSETKQGG 60

Query: 61 VKRGSVSALGRRGQDRVLE 80
          VK  +VSA GRR QDRVLE
Sbjct: 61 VKICTVSAFGRRDQDRVLE 78

[Close]

BLAST of Spg016532 vs. ExPASy TrEMBL
Match: A0A6J1IKM9 (isoamylase 3, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476487 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 2.2e-15
Identity = 51/79 (64.56%), Postives = 56/79 (70.89%), Query Frame = 0


Query: 1  MLRSLSLHHSAALQLPLFASSCTGTPRFHQLRLKPQGRRAAGGGSIRSSQEFLSETKEGS 60
          M+RS+ LHHSA LQLPLFASSCTG     Q RLKPQG+R  G     SSQE  SETK+G 
Sbjct: 1  MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRGPGSS---SSQEVFSETKQGG 60

Query: 61 VKRGSVSALGRRGQDRVLE 80
          VK  +VSA GRR QD VLE
Sbjct: 61 VKICTVSAFGRRDQDHVLE 76

[Close]

BLAST of Spg016532 vs. ExPASy TrEMBL
Match: A0A1S3CS22 (isoamylase 3, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503684 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.1e-11
Identity = 45/79 (56.96%), Postives = 51/79 (64.56%), Query Frame = 0


Query: 1  MLRSLSLHHSAALQLPLFASSCTGTPRFHQLRLKPQGRRAAGGGSIRSSQEFLSETKEGS 60
          M+RSL LHHS  LQ PLF SSCTG   F   RLK Q +  +G  S   S+E  SETK+G 
Sbjct: 1  MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSS-SDSREIFSETKQGG 60

Query: 61 VKRGSVSALGRRGQDRVLE 80
          VK  +VSA  RR QDRVLE
Sbjct: 61 VKLWTVSAFSRRDQDRVLE 78

[Close]

BLAST of Spg016532 vs. ExPASy TrEMBL
Match: A0A5A7TAT5 (Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G002290 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.1e-11
Identity = 45/79 (56.96%), Postives = 51/79 (64.56%), Query Frame = 0


Query: 1  MLRSLSLHHSAALQLPLFASSCTGTPRFHQLRLKPQGRRAAGGGSIRSSQEFLSETKEGS 60
          M+RSL LHHS  LQ PLF SSCTG   F   RLK Q +  +G  S   S+E  SETK+G 
Sbjct: 1  MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSS-SDSREIFSETKQGG 60

Query: 61 VKRGSVSALGRRGQDRVLE 80
          VK  +VSA  RR QDRVLE
Sbjct: 61 VKLWTVSAFSRRDQDRVLE 78

[Close]

BLAST of Spg016532 vs. ExPASy TrEMBL
Match: A0A5D3E5B0 (Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005950 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.1e-11
Identity = 45/79 (56.96%), Postives = 51/79 (64.56%), Query Frame = 0


Query: 1  MLRSLSLHHSAALQLPLFASSCTGTPRFHQLRLKPQGRRAAGGGSIRSSQEFLSETKEGS 60
          M+RSL LHHS  LQ PLF SSCTG   F   RLK Q +  +G  S   S+E  SETK+G 
Sbjct: 1  MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSS-SDSREIFSETKQGG 60

Query: 61 VKRGSVSALGRRGQDRVLE 80
          VK  +VSA  RR QDRVLE
Sbjct: 61 VKLWTVSAFSRRDQDRVLE 78

The following BLAST results are available for this feature:

BLAST of Spg016532 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022936809.1	1.4e-16	68.35	isoamylase 3, chloroplastic isoform X1 [Cucurbita moschata]	[more]
KAG7024560.1	1.4e-16	68.35	Isoamylase 3, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6591680.1	1.4e-16	68.35	Isoamylase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023535183.1	1.6e-15	65.82	isoamylase 3, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_022975928.1	4.5e-15	64.56	isoamylase 3, chloroplastic isoform X1 [Cucurbita maxima]	[more]

BLAST of Spg016532 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg016532 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F9D1	6.8e-17	68.35	isoamylase 3, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1IKM9	2.2e-15	64.56	isoamylase 3, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114764...	[more]
A0A1S3CS22	1.1e-11	56.96	isoamylase 3, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503684 P...	[more]
A0A5A7TAT5	1.1e-11	56.96	Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G002290 P...	[more]
A0A5D3E5B0	1.1e-11	56.96	Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005950 ...	[more]

BLAST of Spg016532 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 35..63
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..22 score: 5.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg016532.1	Spg016532.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds