Spg016475 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016475 vs. NCBI nr
Match: XP_022975992.1 (gamma-tubulin complex component 5-like [Cucurbita maxima])

HSP 1 Score: 1797.7 bits (4655), Expect = 0.0e+00
Identity = 902/991 (91.02%), Postives = 943/991 (95.16%), Query Frame = 0

Query: 1   MEQRKNRSLIDSISDIFTNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60
           MEQRK++SLIDSISDIF+NGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK
Sbjct: 1   MEQRKSKSLIDSISDIFSNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60

Query: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVNSSAKSAPPTLRAFVTSV 120
           KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVL+E+N++AKS PPTLRAFVTSV
Sbjct: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLEEINTTAKSVPPTLRAFVTSV 120

Query: 121 SSWLKRLRDVALKEEIKINDAGSGTTPTLMGLVGSLSSLCSGAEYLLQIVHKAIPKVYFE 180
           S+WLKRLRD  LKEEIKI+DAGSGTTPTLMGL GSLSSLCSGAEYLLQIVHKAIPKV+FE
Sbjct: 121 SAWLKRLRDSVLKEEIKIHDAGSGTTPTLMGLAGSLSSLCSGAEYLLQIVHKAIPKVFFE 180

Query: 181 SSAAITAADLAVHVLDNLYKELDEVCLIQNGQVERTIVEETYQMLLHIFVGSLLPYIEEL 240
           SS A+T ADLAVHVLD+LYK+LDEVCLIQNGQ      EETYQMLLHIFVGSLLPYIEEL
Sbjct: 181 SSGALTTADLAVHVLDSLYKKLDEVCLIQNGQ------EETYQMLLHIFVGSLLPYIEEL 240

Query: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGELSSSIKTEASERES 300
           DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSL+L+GELSSSIK EA+ERES
Sbjct: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLQLEGELSSSIKKEANERES 300

Query: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVCETLSPASEKQNGEEF 360
           ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHV ETLSPASEKQ G+EF
Sbjct: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVYETLSPASEKQYGDEF 360

Query: 361 NGSADYGGSLARLSLSELFCVSLAALIGDGDRISRFFWKHDRYKLETDSLLMSRTNRLEV 420
            GSADYGGSLARLSLSE+FCVSLAALIGDGDRISR+FWKHD+YKLETDSLL S TN+ EV
Sbjct: 361 KGSADYGGSLARLSLSEIFCVSLAALIGDGDRISRYFWKHDQYKLETDSLLKSHTNKFEV 420

Query: 421 ESGISELMCKKKHWYSLLVDASALKGSVCLKSGQKDADKLVGEREKNMTLDMKNCLCSLE 480
            +GI +  CK+KHWYSLLVDA ALK SVCLKSG KDA+KL+GEREKN+T DMKNCLCSLE
Sbjct: 421 ANGIGDSACKEKHWYSLLVDALALKRSVCLKSGHKDANKLIGEREKNITFDMKNCLCSLE 480

Query: 481 VFHPENPVMTVCTTILKDNIDVWKRLNLSRCYNLPPLNDESLLKAIFGDEATPFSATKGT 540
            FHPENPVMTVC TILKDN +VWKRLNLSRC+NLPPLNDESLLKAIFGDE    S TKGT
Sbjct: 481 SFHPENPVMTVCATILKDNTNVWKRLNLSRCFNLPPLNDESLLKAIFGDEDASCSETKGT 540

Query: 541 DFTFGFQFDKYEYRHSQNEAKLIETLFPFPTILPAFQDDVHISDLLPFQKNSTLPSRVLS 600
           DFTFGFQFDKYE+ H + EAKLIETLFPFPTILP F+DD+HISDLLPFQKNSTLPSRVLS
Sbjct: 541 DFTFGFQFDKYEHIHLKKEAKLIETLFPFPTILPEFRDDLHISDLLPFQKNSTLPSRVLS 600

Query: 601 WMQNIVPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660
           WM NIVPRTMPL MVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG
Sbjct: 601 WMSNIVPRTMPLMMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660

Query: 661 SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVKSNS 720
           SGDLLQHF TVIFNKLDKGETWDDDFELNTILQES+RNS+DGMLLSAPDSLVVSIVK+NS
Sbjct: 661 SGDLLQHFFTVIFNKLDKGETWDDDFELNTILQESVRNSSDGMLLSAPDSLVVSIVKTNS 720

Query: 721 LDGDDQSNLVKLPSIPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780
           LDGD+ SNLVK PS PHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF
Sbjct: 721 LDGDEHSNLVKPPSTPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780

Query: 781 LLKVKRAKFVLDKTRRWMWKGKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840
           LLKVKRAKFVLDKTRRWMWKGK TVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR
Sbjct: 781 LLKVKRAKFVLDKTRRWMWKGKGTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840

Query: 841 ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVVLGLALDFYSVQQ 900
           ELCEGMAAAQSLDGVIEVHEAYLL+IHRQCFVVPDKLWALIASRINV+LGLALDFYSVQQ
Sbjct: 841 ELCEGMAAAQSLDGVIEVHEAYLLTIHRQCFVVPDKLWALIASRINVILGLALDFYSVQQ 900

Query: 901 TLSSGGAVSAIKARCEMDVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY 960
           TLSSGGAVSAIKARCEM+VDRIEKQFDDCIAFLLRVLSF LNVGHFPH+ADLVTRINYSY
Sbjct: 901 TLSSGGAVSAIKARCEMEVDRIEKQFDDCIAFLLRVLSFNLNVGHFPHIADLVTRINYSY 960

Query: 961 FYMSDSGNLRTAPSSETVSSRLGKAFMGRTD 992
           FYMSDSGNLRTAPSSETVSSRLGK FMGRTD
Sbjct: 961 FYMSDSGNLRTAPSSETVSSRLGKTFMGRTD 985

BLAST of Spg016475 vs. NCBI nr
Match: XP_022936980.1 (gamma-tubulin complex component 5-like [Cucurbita moschata])

HSP 1 Score: 1793.5 bits (4644), Expect = 0.0e+00
Identity = 901/991 (90.92%), Postives = 939/991 (94.75%), Query Frame = 0

Query: 1   MEQRKNRSLIDSISDIFTNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60
           MEQRK++SLIDSISDIF+NGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK
Sbjct: 1   MEQRKSKSLIDSISDIFSNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60

Query: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVNSSAKSAPPTLRAFVTSV 120
           KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQE+N+SAKS PPTLRAFVTSV
Sbjct: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEINTSAKSVPPTLRAFVTSV 120

Query: 121 SSWLKRLRDVALKEEIKINDAGSGTTPTLMGLVGSLSSLCSGAEYLLQIVHKAIPKVYFE 180
           S WLKRLRD  LKEEIKI+DAGSGTTPTLMGL GSLSSLCSGAEYLLQIVHKAIPKV+FE
Sbjct: 121 SGWLKRLRDSVLKEEIKIHDAGSGTTPTLMGLAGSLSSLCSGAEYLLQIVHKAIPKVFFE 180

Query: 181 SSAAITAADLAVHVLDNLYKELDEVCLIQNGQVERTIVEETYQMLLHIFVGSLLPYIEEL 240
           SS A+T ADLAVHVLD+LYK+LDEVCLIQNGQ      EETYQMLLHIFVGSLLPYIEEL
Sbjct: 181 SSGALTTADLAVHVLDSLYKKLDEVCLIQNGQ------EETYQMLLHIFVGSLLPYIEEL 240

Query: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGELSSSIKTEASERES 300
           DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSL+L+GELSSSIK EA+ERES
Sbjct: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLKLEGELSSSIKKEANERES 300

Query: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVCETLSPASEKQNGEEF 360
           ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHV ETLSPASEKQ G+EF
Sbjct: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVYETLSPASEKQYGDEF 360

Query: 361 NGSADYGGSLARLSLSELFCVSLAALIGDGDRISRFFWKHDRYKLETDSLLMSRTNRLEV 420
           NGSADYGGSLARLSLSE+FC+SLAALIGDGDRISR+FWKHD+YKLETDSL  S TN+ EV
Sbjct: 361 NGSADYGGSLARLSLSEIFCLSLAALIGDGDRISRYFWKHDQYKLETDSLFKSHTNKFEV 420

Query: 421 ESGISELMCKKKHWYSLLVDASALKGSVCLKSGQKDADKLVGEREKNMTLDMKNCLCSLE 480
            +GI +  CK+KHWY+LLVDA ALK  VCLKSG K+A+KL+GEREKN+T DMKNCLCSLE
Sbjct: 421 TNGIGDSACKEKHWYTLLVDALALKRGVCLKSGHKNANKLIGEREKNITFDMKNCLCSLE 480

Query: 481 VFHPENPVMTVCTTILKDNIDVWKRLNLSRCYNLPPLNDESLLKAIFGDEATPFSATKGT 540
            FHPENPVMTVCTTILKDN +VWKRLNLSRC+NLPPLNDESLLKAIFGDE    S TKGT
Sbjct: 481 SFHPENPVMTVCTTILKDNTNVWKRLNLSRCFNLPPLNDESLLKAIFGDEDASCSETKGT 540

Query: 541 DFTFGFQFDKYEYRHSQNEAKLIETLFPFPTILPAFQDDVHISDLLPFQKNSTLPSRVLS 600
           DFTFGFQFDKYE+ H Q EAKLIETLFPFPTILP F+DD+HISDLLPFQKNSTLPSRVLS
Sbjct: 541 DFTFGFQFDKYEHVHLQKEAKLIETLFPFPTILPEFRDDLHISDLLPFQKNSTLPSRVLS 600

Query: 601 WMQNIVPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660
           WM N VPRTMPL MVIMEECLVVYL QQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG
Sbjct: 601 WMSNTVPRTMPLMMVIMEECLVVYLGQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660

Query: 661 SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVKSNS 720
           SGDLLQHF TVIFNKLDKGETWDDDFELNTILQES+RNS+DGMLLSAPDSLVVSIVK+NS
Sbjct: 661 SGDLLQHFFTVIFNKLDKGETWDDDFELNTILQESVRNSSDGMLLSAPDSLVVSIVKTNS 720

Query: 721 LDGDDQSNLVKLPSIPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780
           LDGD+ SNLVK P  PHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF
Sbjct: 721 LDGDEHSNLVKPPLTPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780

Query: 781 LLKVKRAKFVLDKTRRWMWKGKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840
           LLKVKRAKFVLDKTRRWMWKGK TVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR
Sbjct: 781 LLKVKRAKFVLDKTRRWMWKGKGTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840

Query: 841 ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVVLGLALDFYSVQQ 900
           ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINV+LGLALDFYSVQQ
Sbjct: 841 ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVILGLALDFYSVQQ 900

Query: 901 TLSSGGAVSAIKARCEMDVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY 960
           TLSSGGAVSAIKARCEM+VDRIEKQFDDCIAFLLRVLSF LNVGHFPHLADLVTRINYSY
Sbjct: 901 TLSSGGAVSAIKARCEMEVDRIEKQFDDCIAFLLRVLSFNLNVGHFPHLADLVTRINYSY 960

Query: 961 FYMSDSGNLRTAPSSETVSSRLGKAFMGRTD 992
           FYMSDSGNLRTAPSSETVSSRLGK FMGRTD
Sbjct: 961 FYMSDSGNLRTAPSSETVSSRLGKTFMGRTD 985

BLAST of Spg016475 vs. NCBI nr
Match: KAG6591356.1 (Gamma-tubulin complex component 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1792.3 bits (4641), Expect = 0.0e+00
Identity = 900/991 (90.82%), Postives = 938/991 (94.65%), Query Frame = 0

Query: 1   MEQRKNRSLIDSISDIFTNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60
           MEQRK++SLIDSISDIF+NGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK
Sbjct: 1   MEQRKSKSLIDSISDIFSNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60

Query: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVNSSAKSAPPTLRAFVTSV 120
           KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQE+N++AKS PPTLRAFVTSV
Sbjct: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEINTTAKSVPPTLRAFVTSV 120

Query: 121 SSWLKRLRDVALKEEIKINDAGSGTTPTLMGLVGSLSSLCSGAEYLLQIVHKAIPKVYFE 180
           S WLKRLRD  LKEEIKI+DAGSGTTPTLMGL GSLSSLCSGAEYLLQIVHKAIPKV+FE
Sbjct: 121 SGWLKRLRDSVLKEEIKIHDAGSGTTPTLMGLAGSLSSLCSGAEYLLQIVHKAIPKVFFE 180

Query: 181 SSAAITAADLAVHVLDNLYKELDEVCLIQNGQVERTIVEETYQMLLHIFVGSLLPYIEEL 240
           SS A+T ADLAVHVLD+LYK+LDEVCLIQNGQ      EETYQMLLHIFVGSLLPYIEEL
Sbjct: 181 SSGALTTADLAVHVLDSLYKKLDEVCLIQNGQ------EETYQMLLHIFVGSLLPYIEEL 240

Query: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGELSSSIKTEASERES 300
           DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSL+L+GELSSSIK EA+ERES
Sbjct: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLKLEGELSSSIKKEANERES 300

Query: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVCETLSPASEKQNGEEF 360
           ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHV ETLSPASEKQ G+EF
Sbjct: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVYETLSPASEKQYGDEF 360

Query: 361 NGSADYGGSLARLSLSELFCVSLAALIGDGDRISRFFWKHDRYKLETDSLLMSRTNRLEV 420
           NG ADYGGSLARLSLSE+FC+SLAALIGDGDRISR+FWKHD+YKLETDSL  S TN+ EV
Sbjct: 361 NGIADYGGSLARLSLSEIFCLSLAALIGDGDRISRYFWKHDQYKLETDSLFKSHTNKFEV 420

Query: 421 ESGISELMCKKKHWYSLLVDASALKGSVCLKSGQKDADKLVGEREKNMTLDMKNCLCSLE 480
            +GI +  CK+KHWYSLLVDA ALK   CLKSG KDA+KL+GEREKN+T DMK+CLCSLE
Sbjct: 421 TNGIGDSACKEKHWYSLLVDALALKRGACLKSGHKDANKLIGEREKNITFDMKHCLCSLE 480

Query: 481 VFHPENPVMTVCTTILKDNIDVWKRLNLSRCYNLPPLNDESLLKAIFGDEATPFSATKGT 540
            FHPENPVMTVCTTILKDN +VWKRLNLSRC+NLPPLNDESLLKAIFGDE    S TKGT
Sbjct: 481 SFHPENPVMTVCTTILKDNTNVWKRLNLSRCFNLPPLNDESLLKAIFGDEDASCSETKGT 540

Query: 541 DFTFGFQFDKYEYRHSQNEAKLIETLFPFPTILPAFQDDVHISDLLPFQKNSTLPSRVLS 600
           DFTFGFQFDKYE+ H Q EAKLIETLFPFPTILP F+DD+HISDLLPFQKNSTLPSRVLS
Sbjct: 541 DFTFGFQFDKYEHVHLQKEAKLIETLFPFPTILPEFRDDLHISDLLPFQKNSTLPSRVLS 600

Query: 601 WMQNIVPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660
           WM N VPRTMPL MVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG
Sbjct: 601 WMSNTVPRTMPLMMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660

Query: 661 SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVKSNS 720
           SGDLLQHF TVIFNKLDKGETWDDDFELNTILQES+RNS+DGMLLSAPDSLVVSIVK+NS
Sbjct: 661 SGDLLQHFFTVIFNKLDKGETWDDDFELNTILQESVRNSSDGMLLSAPDSLVVSIVKTNS 720

Query: 721 LDGDDQSNLVKLPSIPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780
           LDGD+ SNLVK P  PHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF
Sbjct: 721 LDGDEHSNLVKPPLTPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780

Query: 781 LLKVKRAKFVLDKTRRWMWKGKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840
           LLKVKRAKFVLDKTRRWMWKGK TVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR
Sbjct: 781 LLKVKRAKFVLDKTRRWMWKGKGTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840

Query: 841 ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVVLGLALDFYSVQQ 900
           ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINV+LGLALDFYSVQQ
Sbjct: 841 ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVILGLALDFYSVQQ 900

Query: 901 TLSSGGAVSAIKARCEMDVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY 960
           TLSSGGAVSAIKARCEM+VDRIEKQFDDCIAFLLRVLSF LNVGHFPHLADLVTRINYSY
Sbjct: 901 TLSSGGAVSAIKARCEMEVDRIEKQFDDCIAFLLRVLSFNLNVGHFPHLADLVTRINYSY 960

Query: 961 FYMSDSGNLRTAPSSETVSSRLGKAFMGRTD 992
           FYMSDSGNLRTAPSSETVSSRLGK FMGRTD
Sbjct: 961 FYMSDSGNLRTAPSSETVSSRLGKTFMGRTD 985

BLAST of Spg016475 vs. NCBI nr
Match: XP_023535878.1 (gamma-tubulin complex component 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1791.2 bits (4638), Expect = 0.0e+00
Identity = 901/991 (90.92%), Postives = 939/991 (94.75%), Query Frame = 0

Query: 1   MEQRKNRSLIDSISDIFTNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60
           MEQRK++SLIDSISDIF+NGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWD SGK
Sbjct: 1   MEQRKSKSLIDSISDIFSNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDYSGK 60

Query: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVNSSAKSAPPTLRAFVTSV 120
           KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQE+N++AKS PPTLRAFVTSV
Sbjct: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEINTTAKSVPPTLRAFVTSV 120

Query: 121 SSWLKRLRDVALKEEIKINDAGSGTTPTLMGLVGSLSSLCSGAEYLLQIVHKAIPKVYFE 180
           S WLKRLRD  LKEEIKI+DAGSGTTPTLMGL GSLSSLCSGAEYLLQIVHKAIPKV+FE
Sbjct: 121 SGWLKRLRDSVLKEEIKIHDAGSGTTPTLMGLAGSLSSLCSGAEYLLQIVHKAIPKVFFE 180

Query: 181 SSAAITAADLAVHVLDNLYKELDEVCLIQNGQVERTIVEETYQMLLHIFVGSLLPYIEEL 240
           SS A+T ADLAVHVLD+LYK+LDEVCLIQNGQ      EETYQMLLHIFVGSLLPYIEEL
Sbjct: 181 SSGALTTADLAVHVLDSLYKKLDEVCLIQNGQ------EETYQMLLHIFVGSLLPYIEEL 240

Query: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGELSSSIKTEASERES 300
           DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSL+L+ ELSSSIK EA+ERES
Sbjct: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLKLEDELSSSIKKEANERES 300

Query: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVCETLSPASEKQNGEEF 360
           ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHV ETLSPASEKQ G+EF
Sbjct: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVYETLSPASEKQYGDEF 360

Query: 361 NGSADYGGSLARLSLSELFCVSLAALIGDGDRISRFFWKHDRYKLETDSLLMSRTNRLEV 420
           NGSADYGGSLARLSLSE+FC+SLAALIGDGDRISR+FWKHD Y+LETDSL  S TN+ EV
Sbjct: 361 NGSADYGGSLARLSLSEIFCLSLAALIGDGDRISRYFWKHDHYQLETDSLFKSHTNKFEV 420

Query: 421 ESGISELMCKKKHWYSLLVDASALKGSVCLKSGQKDADKLVGEREKNMTLDMKNCLCSLE 480
            +GI +  CK+KHWYSLLVDA ALK SVCLKSG KDA+KL+GEREKN+T DMKNCLCSLE
Sbjct: 421 TNGIGDSACKEKHWYSLLVDALALKRSVCLKSGHKDANKLIGEREKNITFDMKNCLCSLE 480

Query: 481 VFHPENPVMTVCTTILKDNIDVWKRLNLSRCYNLPPLNDESLLKAIFGDEATPFSATKGT 540
            FHPENPVMTVCTTILKDN +VWKRLNLSRC+NLPPLNDESLLKAIFGDE    S TKGT
Sbjct: 481 SFHPENPVMTVCTTILKDNTNVWKRLNLSRCFNLPPLNDESLLKAIFGDEDASCSETKGT 540

Query: 541 DFTFGFQFDKYEYRHSQNEAKLIETLFPFPTILPAFQDDVHISDLLPFQKNSTLPSRVLS 600
           DFTFGFQFDKYE+ H Q EAKLIETLFPFPTILP F+DD+HISDLLPFQKNSTLPSRVLS
Sbjct: 541 DFTFGFQFDKYEHVHLQKEAKLIETLFPFPTILPEFRDDLHISDLLPFQKNSTLPSRVLS 600

Query: 601 WMQNIVPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660
           WM N VPRTMPL MVIMEECLVVYLRQQVD+IGKRVLSKLMNEWRLMDELAVLRAIYLLG
Sbjct: 601 WMSNTVPRTMPLMMVIMEECLVVYLRQQVDHIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660

Query: 661 SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVKSNS 720
           SGDLLQHF TVIFNKLDKGETWDDDFELNTILQES+RNS+DGMLLSAPDSLVVSIVK+NS
Sbjct: 661 SGDLLQHFFTVIFNKLDKGETWDDDFELNTILQESVRNSSDGMLLSAPDSLVVSIVKTNS 720

Query: 721 LDGDDQSNLVKLPSIPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780
           LDGD+ SNLVK PS PHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF
Sbjct: 721 LDGDEHSNLVKPPSTPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780

Query: 781 LLKVKRAKFVLDKTRRWMWKGKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840
           LLKVKRAKFVLDKTRRWMWKGK TVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR
Sbjct: 781 LLKVKRAKFVLDKTRRWMWKGKGTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840

Query: 841 ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVVLGLALDFYSVQQ 900
           ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINV+LGLALDFYSVQQ
Sbjct: 841 ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVILGLALDFYSVQQ 900

Query: 901 TLSSGGAVSAIKARCEMDVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY 960
           TLSSGGAVSAIKARCEM+VDRIEKQFDDCIAFLLRVLSF LNVGHFPHLADLVTRINYSY
Sbjct: 901 TLSSGGAVSAIKARCEMEVDRIEKQFDDCIAFLLRVLSFNLNVGHFPHLADLVTRINYSY 960

Query: 961 FYMSDSGNLRTAPSSETVSSRLGKAFMGRTD 992
           FYMSDSGNLRTAPSSETVSSRLGK FMGRTD
Sbjct: 961 FYMSDSGNLRTAPSSETVSSRLGKTFMGRTD 985

BLAST of Spg016475 vs. NCBI nr
Match: XP_038897897.1 (gamma-tubulin complex component 5-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1781.9 bits (4614), Expect = 0.0e+00
Identity = 898/991 (90.62%), Postives = 931/991 (93.95%), Query Frame = 0

Query: 1   MEQRKNRSLIDSISDIFTNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60
           MEQ K++SLIDSISDIFTN IHFAAPISSLRTSELDLVRGVLQMLQGFSG+LFSWDCSGK
Sbjct: 1   MEQMKSKSLIDSISDIFTNDIHFAAPISSLRTSELDLVRGVLQMLQGFSGALFSWDCSGK 60

Query: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVNSSAKSAPPTLRAFVTSV 120
           KF AKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVN++AKSAPPTLRAFVTSV
Sbjct: 61  KFHAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVNTTAKSAPPTLRAFVTSV 120

Query: 121 SSWLKRLRDVALKEEIKINDAGSGTTPTLMGLVGSLSSLCSGAEYLLQIVHKAIPKVYFE 180
           S+WLKRLRDVALKEEIK+NDA SGTTPTLMGL GSLSSLCSGAEYLLQIVH AIPKV+FE
Sbjct: 121 SAWLKRLRDVALKEEIKLNDADSGTTPTLMGLAGSLSSLCSGAEYLLQIVHVAIPKVFFE 180

Query: 181 SSAAITAADLAVHVLDNLYKELDEVCLIQNGQVERTIVEETYQMLLHIFVGSLLPYIEEL 240
           SSAAIT ADLAVHVLDNLYK+LDEVCLIQNGQVERTIVEETYQMLLHIFVGSLLPYIEEL
Sbjct: 181 SSAAITTADLAVHVLDNLYKKLDEVCLIQNGQVERTIVEETYQMLLHIFVGSLLPYIEEL 240

Query: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGELSSSIKTEASERES 300
           DSWVFEGILDDPFEELFFYANEAVSV EHDFWEKSYSLRSLRLDGEL  SIK E  ERES
Sbjct: 241 DSWVFEGILDDPFEELFFYANEAVSVGEHDFWEKSYSLRSLRLDGELYLSIKKETIERES 300

Query: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVCETLSPASEKQNGEEF 360
           IS SHLLKGKDQY GGSIACPLF+K IAKSIVAAGKSLQLIRHVCETL+PASEK NGEEF
Sbjct: 301 ISSSHLLKGKDQYAGGSIACPLFIKGIAKSIVAAGKSLQLIRHVCETLTPASEKHNGEEF 360

Query: 361 NGSADYGGSLARLSLSELFCVSLAALIGDGDRISRFFWKHDRYKLETDSLLMSRTNRLEV 420
           N SAD+GGSLARLSLSELFCVSLAALIGDGD ISR+FWKHD+YKLETDS   SR N  EV
Sbjct: 361 NSSADFGGSLARLSLSELFCVSLAALIGDGDHISRYFWKHDQYKLETDSSFKSRVNGFEV 420

Query: 421 ESGISELMCKKKHWYSLLVDASALKGSVCLKSGQKDADKLVGEREKNMTLDMKNCLCSLE 480
           E+GI E  C++KHWYSLL DA ALKGSV LKSG KD + LVGE  KNM+ D KNCLCSLE
Sbjct: 421 ENGIDESTCRRKHWYSLLADALALKGSVSLKSGHKDVNNLVGEGAKNMSFDKKNCLCSLE 480

Query: 481 VFHPENPVMTVCTTILKDNIDVWKRLNLSRCYNLPPLNDESLLKAIFGDEATPFSATKGT 540
            F+PENPVMTVCTTILKDNI+VWKRLNLSRCYNLPPLNDE LLKAIFGDE  P S  KGT
Sbjct: 481 SFYPENPVMTVCTTILKDNINVWKRLNLSRCYNLPPLNDEGLLKAIFGDEDAPISEAKGT 540

Query: 541 DFTFGFQFDKYEYRHSQNEAKLIETLFPFPTILPAFQDDVHISDLLPFQKNSTLPSRVLS 600
           DFT GFQFDKYEY HSQNEAKLIETLFPFPT+LPAFQDD+H+SDLLPFQKNSTLPSRVLS
Sbjct: 541 DFTLGFQFDKYEYVHSQNEAKLIETLFPFPTLLPAFQDDLHVSDLLPFQKNSTLPSRVLS 600

Query: 601 WMQNIVPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660
           WMQN+VPRTMPLT VIMEECLV+YLRQQVDYIG RVLSKLMNEWRLMDELAVLRAIYLLG
Sbjct: 601 WMQNVVPRTMPLTTVIMEECLVIYLRQQVDYIGNRVLSKLMNEWRLMDELAVLRAIYLLG 660

Query: 661 SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVKSNS 720
           SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVK+NS
Sbjct: 661 SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVKTNS 720

Query: 721 LDGDDQSNLVKLPSIPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780
           LDGD+QSNL KLP  PHKSSA GFG+DGLDSLKF YKVSWPLELIANTEAIKKYNQVTGF
Sbjct: 721 LDGDEQSNLAKLPLTPHKSSALGFGIDGLDSLKFAYKVSWPLELIANTEAIKKYNQVTGF 780

Query: 781 LLKVKRAKFVLDKTRRWMWKGKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840
           LLKVKRAKFVLDKTRRWMWKGK T  NN KRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR
Sbjct: 781 LLKVKRAKFVLDKTRRWMWKGKGTPKNNSKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840

Query: 841 ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVVLGLALDFYSVQQ 900
           ELCEGMAAAQSLDGVIEVHEAYLL+IHRQCFVVPDKLWALIASRINV+LGLALDFYSVQQ
Sbjct: 841 ELCEGMAAAQSLDGVIEVHEAYLLTIHRQCFVVPDKLWALIASRINVILGLALDFYSVQQ 900

Query: 901 TLSSGGAVSAIKARCEMDVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY 960
           TLSSGGAVSAIK RCEM+VDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY
Sbjct: 901 TLSSGGAVSAIKLRCEMEVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY 960

Query: 961 FYMSDSGNLRTAPSSETVSSRLGKAFMGRTD 992
           FYMSDSGNLRTAPS+ETVSSRLGK FMGRTD
Sbjct: 961 FYMSDSGNLRTAPSAETVSSRLGKGFMGRTD 991

BLAST of Spg016475 vs. ExPASy Swiss-Prot
Match: Q8BKN5 (Gamma-tubulin complex component 5 OS=Mus musculus OX=10090 GN=Tubgcp5 PE=2 SV=2)

HSP 1 Score: 160.2 bits (404), Expect = 1.2e-37
Identity = 219/971 (22.55%), Postives = 378/971 (38.93%), Query Frame = 0

Query: 26   PISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGKKFCAKSGIYVSHLSRSSLLAILNQF 85
            P   +  +E  ++R  L +L G    +F +     K   ++ I V+HL+ S L ++L Q 
Sbjct: 259  PDDRVVVTETQVIRETLWLLSGVK-KMFIFQLIDGKVTVRNNIIVTHLTHSCLRSVLEQI 318

Query: 86   -MYAATCLQLTQLVLQEVNSSAKSAPP---------------TLRAFVTSVSSWLKRLRD 145
              Y     +L + + + +  S++S PP               T +AF+ ++  +      
Sbjct: 319  AAYGQVVFRLQEFIDEVMGHSSESLPPGNGPIPKKQPDAPFRTYQAFMWALYKYF----- 378

Query: 146  VALKEEIKIND---AGSGTTPTLMGLVGSLSSLCSGAEYLLQIVHKAIPKVYFESSAAIT 205
            +  KEE+   +     S TT TL  +V  L+   +  + L ++    + +V  ++   + 
Sbjct: 379  INFKEELTDIEKCVISSDTTITLAIVVNKLAPRLAQLKVLDKVFSTGVAEVPPDTRNVVR 438

Query: 206  AADLAVHVLDNLYKELDEVCLIQNGQVERTIVEETYQMLLHIFVGSLLPYIEELDSWVFE 265
            A+    H+L+ LYK + E   +          E+T  +L  ++V ++ PY++ +D W+  
Sbjct: 439  AS----HLLNTLYKAILEYDNVGEAS------EQTVSLLFSLWVETVRPYLQTVDEWIVH 498

Query: 266  GILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGELSSSIKTEASERESISLSHL 325
            G L D   E     N+ V V+  DFW  +Y+L S+        S KTE  ++ S S S  
Sbjct: 499  GHLWDGAREFIIQRNKNVPVNHRDFWYATYTLYSV--------SEKTENEDKVSDSAS-A 558

Query: 326  LKGKDQ-YTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVCETLSPASEKQNGEEFNGSAD 385
              G DQ  +        F+K + K I+ AGKS+QL++++     PA +            
Sbjct: 559  SSGSDQGPSSRQHTMVSFLKPVLKQIIMAGKSMQLLKNLNCAEGPACQ------------ 618

Query: 386  YGGSLARLSLSELFCVSLAALIGDGDRISRFFWKHDRYKLETDSLLMSRTNRLEVESGIS 445
                               A   D +R                                 
Sbjct: 619  -------------------AAARDAER--------------------------------- 678

Query: 446  ELMCKKKHWYSLLVDASALKGSVCLKSGQKDADKLVGEREKNMTLDMKNCLCSLEVFHPE 505
                  K  Y+L +++  L+    L+ G+  A  +V E +      +K  + S+   H E
Sbjct: 679  ------KSLYTLFLESIQLR----LQHGEDSAPHIVNEDQTTKENLIK--MQSIAERHLE 738

Query: 506  NPVMTVCTTILKDNIDVWKRLNLSRCYNLPPLNDESLLKAIFGDEATPFSATKGTDFTFG 565
                      L D  D    +N +R Y                                 
Sbjct: 739  ----------LDDIHDPLLAINFARLY--------------------------------- 798

Query: 566  FQFDKYEYRHSQNEAKLIETLFPFPTILPAFQDDVHISDLLPFQKNSTLPSRVLSWMQNI 625
                                           Q D H       +K +     V    +++
Sbjct: 799  -----------------------------LEQSDFH-------EKFAGGDICVDRSSESV 858

Query: 626  VPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLGSGDLL 685
              +T  LT   +  CL  ++ +Q  +    ++  L  ++RL++ L  +R  +L+  GD +
Sbjct: 859  TCQTFELT---LRSCLYPHIDKQYLHCCGNLMQTLKRDFRLVEYLQAMRNFFLMEGGDTM 918

Query: 686  QHFLTVIFNKLDKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVKSNSLDGDD 745
              F T IF+K+ + ETW +   LN  LQE++            DSL +SI   N      
Sbjct: 919  YDFYTSIFDKIREKETWQNVSFLNVQLQEAVGQR------YPEDSLRLSISFENV----- 978

Query: 746  QSNLVKLPSIPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGFLLKVK 805
             +   KLP            +  LD L  +YKV WP++++ + E  K YNQV   LL++K
Sbjct: 979  DTTKKKLP------------VHILDGLTLSYKVPWPVDIVISVECQKIYNQVFLLLLQIK 1013

Query: 806  RAKFVLD------------------------KTRRWMWKGKSTVVNNCKRHWLVEQKLLH 865
             AK+ LD                         T       K ++     R +L+  KL+H
Sbjct: 1039 WAKYSLDVLLFGELGNAAERSQAKEDIPRDQDTPSQFGPPKESLRQQIHRMFLLRVKLMH 1013

Query: 866  FVDAFHQYVMDRVYHSAWRELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALI 925
            FV++ H Y+M R+ HS   E    +  A+ LD +I++H  YL +IH +C ++ +K+ + +
Sbjct: 1099 FVNSLHNYIMTRILHSTGLEFQHQVEEAKDLDQLIKIHYRYLSTIHDRC-LLREKV-SFV 1013

Query: 926  ASRINVVLGLALDFYSVQQTLSSGGAVSAIKARCEMDVDRIEKQFDDCIAFLLRVLSFKL 953
               I  VL LAL F        + G  + + A     ++++E  F +C  FL+ +L+  +
Sbjct: 1159 KEAIMKVLNLALMF--------AEGWQAGLGAWQMESIEKMESDFKNCHMFLVTILNKAV 1013

BLAST of Spg016475 vs. ExPASy Swiss-Prot
Match: Q96RT8 (Gamma-tubulin complex component 5 OS=Homo sapiens OX=9606 GN=TUBGCP5 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 1.4e-35
Identity = 225/973 (23.12%), Postives = 374/973 (38.44%), Query Frame = 0

Query: 26   PISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGKKFCAKSGIYVSHLSRSSLLAILNQF 85
            P   +  +E  ++R  L +L G    LF +     K   ++ I V+HL+ S L ++L Q 
Sbjct: 259  PDDRVLVTETQVIRETLWLLSGVK-KLFIFQLIDGKVTVRNNIIVTHLTHSCLRSVLEQI 318

Query: 86   -MYAATCLQLTQLVLQEVNSSAKSAPP---------------TLRAFVTSVSSWLKRLRD 145
              Y     +L + + + +  S++S  P               T +AF+ ++  +      
Sbjct: 319  AAYGQVVFRLQEFIDEVMGHSSESMLPGSGSVPKKSTEAPFRTYQAFMWALYKYF----- 378

Query: 146  VALKEEIKIND---AGSGTTPTLMGLVGSLSSLCSGAEYLLQIVHKAIPKVYFESSAAIT 205
            ++ KEE+   +     + TT TL  +V  L+   S  + L ++    + +V  ++   + 
Sbjct: 379  ISFKEELAEIEKCIINNDTTITLAIVVDKLAPRLSQLKVLHKVFSTGVAEVPPDTRNVVR 438

Query: 206  AADLAVHVLDNLYKELDEVCLIQNGQVERTIVEETYQMLLHIFVGSLLPYIEELDSWVFE 265
            A+    H+L+ LYK + E   +          E+T  +L  ++V ++ PY++ +D W+  
Sbjct: 439  AS----HLLNTLYKAILEYDNVGEAS------EQTVSLLFSLWVETVRPYLQTVDEWIVH 498

Query: 266  GILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGELSSSIKTEASERESISLSHL 325
            G L D   E     N+ V V+  DFW  +Y+L S+        S KTE  E+ S + S  
Sbjct: 499  GHLWDGAREFIIQRNKNVPVNHRDFWYATYTLYSV--------SEKTENEEKMSDNAS-A 558

Query: 326  LKGKDQ-YTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVCETLSPASEKQNGEEFNGSAD 385
              G DQ  +        F+K + K I+ AGKS+QL+++                      
Sbjct: 559  SSGSDQGPSSRQHTMVSFLKPVLKQIIMAGKSMQLLKN---------------------- 618

Query: 386  YGGSLARLSLSELFCVSLAALIGDGDRISRFFWKHDRYKLETDSLLMSRTNRLEVESGIS 445
                   L  +E    S     G  D                                  
Sbjct: 619  -------LQCAE----STTCQAGARD---------------------------------- 678

Query: 446  ELMCKKKHWYSLLVDASALKGSVCLKSGQKDADKLVGEREKNMTLDMKNCLCSLEVFHPE 505
                ++K  Y+L +++   +    L+ G+    +++ E++      MK  + S+   H E
Sbjct: 679  ---AERKSLYTLFLESVQSR----LRHGEDSTPQVLTEQQATKENLMK--MQSIAESHLE 738

Query: 506  NPVMTVCTTILKDNIDVWKRLNLSRCYNLPPLNDESLLKAIFGDEATPFSATKGTDFTFG 565
                      L D  D    +N +R Y       E   K   GD     S+   T  TF 
Sbjct: 739  ----------LDDVHDPLLAINFARMYLEQSDFHE---KFAGGDVCVDRSSESVTCQTF- 798

Query: 566  FQFDKYEYRHSQNEAKLIETLFPFPTILPAFQDDVHISDLLPFQKNSTLPSRVLSWMQNI 625
                         E  L   L+P            HI                       
Sbjct: 799  -------------ELTLRSCLYP------------HID---------------------- 858

Query: 626  VPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLGSGDLL 685
                                +Q +D  G  ++  L  ++RL++ L  +R  +L+  GD +
Sbjct: 859  --------------------KQYLDCCG-NLMQTLKKDYRLVEYLQAMRNFFLMEGGDTM 918

Query: 686  QHFLTVIFNKLDKGETWDDDFELNTILQESI--RNSADGMLLSAPDSLVVSIVKSNSLDG 745
              F T IF+K+ + ETW +   LN  LQE++  R   D   LS     V +  K      
Sbjct: 919  YDFYTSIFDKIREKETWQNVSFLNVQLQEAVGQRYPEDSSRLSISFENVDTAKK------ 978

Query: 746  DDQSNLVKLPSIPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGFLLK 805
                   KLP            +  LD L  +YKV WP++++ + E  K YNQV   LL+
Sbjct: 979  -------KLP------------VHILDGLTLSYKVPWPVDIVISLECQKIYNQVFLLLLQ 1013

Query: 806  VKRAKFVLD------------------------KTRRWMWKGKSTVVNNCKRHWLVEQKL 865
            +K AK+ LD                         T       K  V     R +L+  KL
Sbjct: 1039 IKWAKYSLDVLLFGELVSTAEKPRLKEGLIHEQDTVAQFGPQKEPVRQQIHRMFLLRVKL 1013

Query: 866  LHFVDAFHQYVMDRVYHSAWRELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWA 925
            +HFV++ H Y+M R+ HS   E    +  A+ LD +I++H  YL +IH +C ++ +K+ +
Sbjct: 1099 MHFVNSLHNYIMTRILHSTGLEFQHQVEEAKDLDQLIKIHYRYLSTIHDRC-LLREKV-S 1013

Query: 926  LIASRINVVLGLALDFYSVQQTLSSGGAVSAIKARCEMDVDRIEKQFDDCIAFLLRVLSF 953
             +   I  VL LAL F       + G        R E  ++++E  F +C  FL+ +L+ 
Sbjct: 1159 FVKEAIMKVLNLALMF-------ADGWQAGLGTWRME-SIEKMESDFKNCHMFLVTILNK 1013

BLAST of Spg016475 vs. ExPASy Swiss-Prot
Match: Q95K09 (Gamma-tubulin complex component 5 (Fragment) OS=Macaca fascicularis OX=9541 GN=TUBGCP5 PE=2 SV=2)

HSP 1 Score: 142.1 bits (357), Expect = 3.3e-32
Identity = 221/932 (23.71%), Postives = 360/932 (38.63%), Query Frame = 0

Query: 68  IYVSHLSRSSLLAILNQF-MYAATCLQLTQLVLQEVNSSAKSAPP--------------- 127
           I V+HL+ S L ++L Q   Y     +L Q + + +  S++S  P               
Sbjct: 1   IIVTHLTHSCLRSVLEQIAAYGQVVFRLQQFIDEVMGHSSESMLPGSGSVPKKSTEAPFR 60

Query: 128 TLRAFVTSVSSW-LKRLRDVALKEEIKINDAGSGTTPTLMGLVGSLSSLCSGAEYLLQIV 187
           T +AF+ ++  + +    ++A  E+  IN   +  T TL  +V  L+   +  + L ++ 
Sbjct: 61  TYQAFMWALYKYFISSKEELAEIEKCIIN---NDATITLAIVVDKLAPRLAQLKVLHKVF 120

Query: 188 HKAIPKVYFESSAAITAADLAVHVLDNLYK---ELDEVCLIQNGQVERTIVEETYQMLLH 247
              + +V  ++   + A+    H+L+ LYK   E D+V     G+      E+T  +L  
Sbjct: 121 STGVAEVPPDTRNVVRAS----HLLNTLYKAILEYDDV-----GEAS----EQTVSLLFS 180

Query: 248 IFVGSLLPYIEELDSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGEL 307
           ++V ++ PY++ +D W+  G L D   E     N+ V V+  DFW  +Y+L S+      
Sbjct: 181 LWVETVRPYLQTVDEWIVHGHLWDGAREFIIQRNKNVPVNHRDFWYATYTLYSV------ 240

Query: 308 SSSIKTEASERESISLSHLLKGKDQ-YTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVCE 367
             S KTE  E+ S + S    G DQ  +        F+K + K I+ AGKS+QL++++  
Sbjct: 241 --SEKTENEEKMSDNAS-ASSGSDQGPSSRQHTMVSFLKPVLKQIIMAGKSMQLLKNLQC 300

Query: 368 TLSPASEKQNGEEFNGSADYGGSLARLSLSELFCVSLAALIGDGDRISRFFWKHDRYKLE 427
             S   +        G+ D      R SL  LF  S+ + +  G+               
Sbjct: 301 AESTTCQA-------GARD----AERKSLYTLFLESVQSRLRHGE--------------- 360

Query: 428 TDSLLMSRTNRLEVESGISELMCKKKHWYSLLVDASALKGSVCLKSGQKDADKLVGEREK 487
            DS                           +L +  A K ++          K+    E+
Sbjct: 361 -DST------------------------PQVLTEQQATKENLM---------KMQSIAER 420

Query: 488 NMTLDMKNCLCSLEVFHPENPVMTVCTTILKDNIDVWKRLNLSRCYNLPPLNDESLLKAI 547
           ++ LD                          D  D    +N +R Y       E   K  
Sbjct: 421 HLELD--------------------------DVHDPLLAINFARMYLEQSDFHE---KFA 480

Query: 548 FGDEATPFSATKGTDFTFGFQFDKYEYRHSQNEAKLIETLFPFPTILPAFQDDVHISDLL 607
            GD     S+   T  TF              E  L   L+P            HI    
Sbjct: 481 GGDVCVDRSSESVTCQTF--------------ELTLRSCLYP------------HID--- 540

Query: 608 PFQKNSTLPSRVLSWMQNIVPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRL 667
                                                  +Q +D  G  ++  L  ++RL
Sbjct: 541 ---------------------------------------KQYLDCCG-NLMQTLKKDYRL 600

Query: 668 MDELAVLRAIYLLGSGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESI--RNSADGML 727
           ++ L  +R  +L+  GD +  F T IF+K+ + ETW +   LN  LQE++  R   D   
Sbjct: 601 VEYLQAMRNFFLMEGGDTMYDFYTSIFDKIREKETWQNVSFLNVQLQEAVGQRYPEDSSR 660

Query: 728 LSAPDSLVVSIVKSNSLDGDDQSNLVKLPSIPHKSSAHGFGMDGLDSLKFTYKVSWPLEL 787
           LS     V +  K             KLP            +  LD L  +YKV WP+++
Sbjct: 661 LSISFENVDTAKK-------------KLP------------VHILDGLTLSYKVPWPVDI 714

Query: 788 IANTEAIKKYNQVTGFLLKVKRAKFVLD------------------------KTRRWMWK 847
           + + E  K YNQV   LL++K AK+ LD                         T      
Sbjct: 721 VISLECQKIYNQVFLLLLQIKWAKYSLDVLLFGELVSTAEKPRLQEGLVREQDTVAQFGP 714

Query: 848 GKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWRELCEGMAAAQSLDGVIEVHE 907
            K  V     R +L+  KL+HFV++ H Y+M R+ HS   E    +  A+ LD +I++H 
Sbjct: 781 QKEPVRQQIHRMFLLRVKLMHFVNSLHNYIMTRILHSTGLEFQHQVEEAKDLDQLIKIHY 714

Query: 908 AYLLSIHRQCFVVPDKLWALIASRINVVLGLALDFYSVQQTLSSGGAVSAIKARCEMDVD 953
            YL +IH +C ++ +K+ + +   I  VL LAL F       + G        R E  ++
Sbjct: 841 RYLSTIHDRC-LLREKV-SFVKEAIMKVLNLALMF-------ADGWQAGLGTWRME-SIE 714

BLAST of Spg016475 vs. ExPASy Swiss-Prot
Match: Q95ZG3 (Spindle pole body component 97 OS=Dictyostelium discoideum OX=44689 GN=spc97 PE=2 SV=2)

HSP 1 Score: 85.1 bits (209), Expect = 4.8e-15
Identity = 87/430 (20.23%), Postives = 175/430 (40.70%), Query Frame = 0

Query: 592  STLPSRVLSWMQNIVPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELA 651
            S +P+ ++ + +N             E   +  + +  DY    +L+ L+NE  L+  L 
Sbjct: 734  SMIPTTIIEYSKN-------------ENVYIDKIEKAYDYASGILLNLLINERHLISRLK 793

Query: 652  VLRAIYLLGSGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSA----------- 711
             ++  +LL  GD   HF+   +++L K     +  ++N++LQ S+R S+           
Sbjct: 794  AIKHYFLLCKGDFFSHFMDTTYDELKKPLDQINMVKMNSLLQLSLRTSSISEEDEFKDDL 853

Query: 712  ----------DGML-------LSAPDSLVVSIVKSNSLDGDDQS-------NLVKLPSIP 771
                      D +L        + P +   +   SN++     +       N     ++ 
Sbjct: 854  ECEFIPYKLVDQLLNIININNFNNPTTTTTTNTSSNNITATTNTSSNNTTINTTVNTTLI 913

Query: 772  HKSSAHGF----GMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGFLLKVKRAKFVLD 831
              +SAH       + G++SL F Y V WPL LI + +++ KY  +   L   K  + VL 
Sbjct: 914  QSTSAHEVLKTNKLFGIESLAFNYNVGWPLSLIISRKSLIKYQIIFRHLFLCKHVEKVLV 973

Query: 832  KT------RRWMWK--GKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWRELCE 891
             T      RR  +   G ST+++      L+  +++HF+     Y+M  V    W ++  
Sbjct: 974  DTWSQHQFRRSSYNKPGLSTLLSFTH---LLRHRMIHFLQNLEYYMMLEVLEPNWNKMKN 1033

Query: 892  GMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVVLGLA------------ 951
             +  ++++D VI +H  +L +   +C +   KL  ++   +++ +  A            
Sbjct: 1034 SIKTSKTVDDVIRIHNDFLETCLTECMLTDTKLVNILMKFMSLCITFANFTNEMMQDEKN 1093

Query: 952  LDFYSVQQTLSSGGAVSAIKARCEMDVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADL 963
            +D   +++T+SS                  E +F   +  L+  L          H+  L
Sbjct: 1094 IDTEQIKKTISSS-----------------ENKFHAILKVLMDTLKSFSKTESNRHMIHL 1130

BLAST of Spg016475 vs. ExPASy Swiss-Prot
Match: Q9D4F8 (Gamma-tubulin complex component 4 OS=Mus musculus OX=10090 GN=Tubgcp4 PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 4.1e-14
Identity = 87/356 (24.44%), Postives = 146/356 (41.01%), Query Frame = 0

Query: 622 VVYLRQQVDYIGKRV---LSKLM-NEWRLMDELAVLRAIYLLGSGDLLQHFLTVIFNKLD 681
           +V   Q VD I   V   L KLM  E  L+ +L +++  YLLG G+L Q F+    + L 
Sbjct: 322 LVDFEQVVDRIRSTVAEHLWKLMVEESDLLGQLKIIKDFYLLGRGELFQAFIDTAQHMLK 381

Query: 682 KGETWDDDFELNTILQESIRNSA--DGMLLSAPDSLVVSIVKSNSLDGDDQSNLVKLPSI 741
              T   + ++N   Q+S       D  LL      +    K +  D      +    + 
Sbjct: 382 TPPTAVTEHDVNVAFQQSAHKVLLDDDNLLPLLHLTIEYHGKDHKADATQPREVPSRETS 441

Query: 742 PHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGFLLKVKRAKFVLDKTR 801
           P ++ + G+   GL     +YKV WPL ++     ++KYN V  +LL V+R +  L    
Sbjct: 442 PREAPSSGWAALGL-----SYKVQWPLHILFTPAVLEKYNVVFKYLLSVRRVQAELQHCW 501

Query: 802 RWMWKGKSTVVNNCKR-HWLVEQKLLHFVDAFHQYVMDRVYHSAWRELCEGMAAAQSLDG 861
               + K    N      W +   +   VD    Y+   V  S + +L   + + +  + 
Sbjct: 502 ALQMQRKHLKSNQTDAVKWRLRNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFES 561

Query: 862 VIEVHEAYLLSIHRQCFVVPDKLWALIASRINVVLGLALDFYS-VQQTLSSGGAVSAIKA 921
           +   H+ +L ++  Q F+    L   +   +N +L L   F S V Q L        +  
Sbjct: 562 IRLAHDHFLSNLLAQSFI----LLKPVFHCLNEILDLCHSFCSLVSQNL------GPLDE 621

Query: 922 RCEMDVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSYFYMSDSGNL 970
           R    +  + K F    + L ++LS   N      LA L+ R++Y+ +Y    G L
Sbjct: 622 RGAAQLSILVKGFSRQSSLLFKILSSVRNHQINSDLAQLLLRLDYNKYYTQAGGTL 662

BLAST of Spg016475 vs. ExPASy TrEMBL
Match: A0A6J1IEK0 (Gamma-tubulin complex component OS=Cucurbita maxima OX=3661 GN=LOC111476527 PE=3 SV=1)

HSP 1 Score: 1797.7 bits (4655), Expect = 0.0e+00
Identity = 902/991 (91.02%), Postives = 943/991 (95.16%), Query Frame = 0

Query: 1   MEQRKNRSLIDSISDIFTNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60
           MEQRK++SLIDSISDIF+NGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK
Sbjct: 1   MEQRKSKSLIDSISDIFSNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60

Query: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVNSSAKSAPPTLRAFVTSV 120
           KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVL+E+N++AKS PPTLRAFVTSV
Sbjct: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLEEINTTAKSVPPTLRAFVTSV 120

Query: 121 SSWLKRLRDVALKEEIKINDAGSGTTPTLMGLVGSLSSLCSGAEYLLQIVHKAIPKVYFE 180
           S+WLKRLRD  LKEEIKI+DAGSGTTPTLMGL GSLSSLCSGAEYLLQIVHKAIPKV+FE
Sbjct: 121 SAWLKRLRDSVLKEEIKIHDAGSGTTPTLMGLAGSLSSLCSGAEYLLQIVHKAIPKVFFE 180

Query: 181 SSAAITAADLAVHVLDNLYKELDEVCLIQNGQVERTIVEETYQMLLHIFVGSLLPYIEEL 240
           SS A+T ADLAVHVLD+LYK+LDEVCLIQNGQ      EETYQMLLHIFVGSLLPYIEEL
Sbjct: 181 SSGALTTADLAVHVLDSLYKKLDEVCLIQNGQ------EETYQMLLHIFVGSLLPYIEEL 240

Query: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGELSSSIKTEASERES 300
           DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSL+L+GELSSSIK EA+ERES
Sbjct: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLQLEGELSSSIKKEANERES 300

Query: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVCETLSPASEKQNGEEF 360
           ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHV ETLSPASEKQ G+EF
Sbjct: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVYETLSPASEKQYGDEF 360

Query: 361 NGSADYGGSLARLSLSELFCVSLAALIGDGDRISRFFWKHDRYKLETDSLLMSRTNRLEV 420
            GSADYGGSLARLSLSE+FCVSLAALIGDGDRISR+FWKHD+YKLETDSLL S TN+ EV
Sbjct: 361 KGSADYGGSLARLSLSEIFCVSLAALIGDGDRISRYFWKHDQYKLETDSLLKSHTNKFEV 420

Query: 421 ESGISELMCKKKHWYSLLVDASALKGSVCLKSGQKDADKLVGEREKNMTLDMKNCLCSLE 480
            +GI +  CK+KHWYSLLVDA ALK SVCLKSG KDA+KL+GEREKN+T DMKNCLCSLE
Sbjct: 421 ANGIGDSACKEKHWYSLLVDALALKRSVCLKSGHKDANKLIGEREKNITFDMKNCLCSLE 480

Query: 481 VFHPENPVMTVCTTILKDNIDVWKRLNLSRCYNLPPLNDESLLKAIFGDEATPFSATKGT 540
            FHPENPVMTVC TILKDN +VWKRLNLSRC+NLPPLNDESLLKAIFGDE    S TKGT
Sbjct: 481 SFHPENPVMTVCATILKDNTNVWKRLNLSRCFNLPPLNDESLLKAIFGDEDASCSETKGT 540

Query: 541 DFTFGFQFDKYEYRHSQNEAKLIETLFPFPTILPAFQDDVHISDLLPFQKNSTLPSRVLS 600
           DFTFGFQFDKYE+ H + EAKLIETLFPFPTILP F+DD+HISDLLPFQKNSTLPSRVLS
Sbjct: 541 DFTFGFQFDKYEHIHLKKEAKLIETLFPFPTILPEFRDDLHISDLLPFQKNSTLPSRVLS 600

Query: 601 WMQNIVPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660
           WM NIVPRTMPL MVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG
Sbjct: 601 WMSNIVPRTMPLMMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660

Query: 661 SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVKSNS 720
           SGDLLQHF TVIFNKLDKGETWDDDFELNTILQES+RNS+DGMLLSAPDSLVVSIVK+NS
Sbjct: 661 SGDLLQHFFTVIFNKLDKGETWDDDFELNTILQESVRNSSDGMLLSAPDSLVVSIVKTNS 720

Query: 721 LDGDDQSNLVKLPSIPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780
           LDGD+ SNLVK PS PHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF
Sbjct: 721 LDGDEHSNLVKPPSTPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780

Query: 781 LLKVKRAKFVLDKTRRWMWKGKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840
           LLKVKRAKFVLDKTRRWMWKGK TVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR
Sbjct: 781 LLKVKRAKFVLDKTRRWMWKGKGTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840

Query: 841 ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVVLGLALDFYSVQQ 900
           ELCEGMAAAQSLDGVIEVHEAYLL+IHRQCFVVPDKLWALIASRINV+LGLALDFYSVQQ
Sbjct: 841 ELCEGMAAAQSLDGVIEVHEAYLLTIHRQCFVVPDKLWALIASRINVILGLALDFYSVQQ 900

Query: 901 TLSSGGAVSAIKARCEMDVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY 960
           TLSSGGAVSAIKARCEM+VDRIEKQFDDCIAFLLRVLSF LNVGHFPH+ADLVTRINYSY
Sbjct: 901 TLSSGGAVSAIKARCEMEVDRIEKQFDDCIAFLLRVLSFNLNVGHFPHIADLVTRINYSY 960

Query: 961 FYMSDSGNLRTAPSSETVSSRLGKAFMGRTD 992
           FYMSDSGNLRTAPSSETVSSRLGK FMGRTD
Sbjct: 961 FYMSDSGNLRTAPSSETVSSRLGKTFMGRTD 985

BLAST of Spg016475 vs. ExPASy TrEMBL
Match: A0A6J1FF89 (Gamma-tubulin complex component OS=Cucurbita moschata OX=3662 GN=LOC111443409 PE=3 SV=1)

HSP 1 Score: 1793.5 bits (4644), Expect = 0.0e+00
Identity = 901/991 (90.92%), Postives = 939/991 (94.75%), Query Frame = 0

Query: 1   MEQRKNRSLIDSISDIFTNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60
           MEQRK++SLIDSISDIF+NGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK
Sbjct: 1   MEQRKSKSLIDSISDIFSNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60

Query: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVNSSAKSAPPTLRAFVTSV 120
           KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQE+N+SAKS PPTLRAFVTSV
Sbjct: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEINTSAKSVPPTLRAFVTSV 120

Query: 121 SSWLKRLRDVALKEEIKINDAGSGTTPTLMGLVGSLSSLCSGAEYLLQIVHKAIPKVYFE 180
           S WLKRLRD  LKEEIKI+DAGSGTTPTLMGL GSLSSLCSGAEYLLQIVHKAIPKV+FE
Sbjct: 121 SGWLKRLRDSVLKEEIKIHDAGSGTTPTLMGLAGSLSSLCSGAEYLLQIVHKAIPKVFFE 180

Query: 181 SSAAITAADLAVHVLDNLYKELDEVCLIQNGQVERTIVEETYQMLLHIFVGSLLPYIEEL 240
           SS A+T ADLAVHVLD+LYK+LDEVCLIQNGQ      EETYQMLLHIFVGSLLPYIEEL
Sbjct: 181 SSGALTTADLAVHVLDSLYKKLDEVCLIQNGQ------EETYQMLLHIFVGSLLPYIEEL 240

Query: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGELSSSIKTEASERES 300
           DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSL+L+GELSSSIK EA+ERES
Sbjct: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLKLEGELSSSIKKEANERES 300

Query: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVCETLSPASEKQNGEEF 360
           ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHV ETLSPASEKQ G+EF
Sbjct: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVYETLSPASEKQYGDEF 360

Query: 361 NGSADYGGSLARLSLSELFCVSLAALIGDGDRISRFFWKHDRYKLETDSLLMSRTNRLEV 420
           NGSADYGGSLARLSLSE+FC+SLAALIGDGDRISR+FWKHD+YKLETDSL  S TN+ EV
Sbjct: 361 NGSADYGGSLARLSLSEIFCLSLAALIGDGDRISRYFWKHDQYKLETDSLFKSHTNKFEV 420

Query: 421 ESGISELMCKKKHWYSLLVDASALKGSVCLKSGQKDADKLVGEREKNMTLDMKNCLCSLE 480
            +GI +  CK+KHWY+LLVDA ALK  VCLKSG K+A+KL+GEREKN+T DMKNCLCSLE
Sbjct: 421 TNGIGDSACKEKHWYTLLVDALALKRGVCLKSGHKNANKLIGEREKNITFDMKNCLCSLE 480

Query: 481 VFHPENPVMTVCTTILKDNIDVWKRLNLSRCYNLPPLNDESLLKAIFGDEATPFSATKGT 540
            FHPENPVMTVCTTILKDN +VWKRLNLSRC+NLPPLNDESLLKAIFGDE    S TKGT
Sbjct: 481 SFHPENPVMTVCTTILKDNTNVWKRLNLSRCFNLPPLNDESLLKAIFGDEDASCSETKGT 540

Query: 541 DFTFGFQFDKYEYRHSQNEAKLIETLFPFPTILPAFQDDVHISDLLPFQKNSTLPSRVLS 600
           DFTFGFQFDKYE+ H Q EAKLIETLFPFPTILP F+DD+HISDLLPFQKNSTLPSRVLS
Sbjct: 541 DFTFGFQFDKYEHVHLQKEAKLIETLFPFPTILPEFRDDLHISDLLPFQKNSTLPSRVLS 600

Query: 601 WMQNIVPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660
           WM N VPRTMPL MVIMEECLVVYL QQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG
Sbjct: 601 WMSNTVPRTMPLMMVIMEECLVVYLGQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660

Query: 661 SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVKSNS 720
           SGDLLQHF TVIFNKLDKGETWDDDFELNTILQES+RNS+DGMLLSAPDSLVVSIVK+NS
Sbjct: 661 SGDLLQHFFTVIFNKLDKGETWDDDFELNTILQESVRNSSDGMLLSAPDSLVVSIVKTNS 720

Query: 721 LDGDDQSNLVKLPSIPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780
           LDGD+ SNLVK P  PHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF
Sbjct: 721 LDGDEHSNLVKPPLTPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780

Query: 781 LLKVKRAKFVLDKTRRWMWKGKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840
           LLKVKRAKFVLDKTRRWMWKGK TVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR
Sbjct: 781 LLKVKRAKFVLDKTRRWMWKGKGTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840

Query: 841 ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVVLGLALDFYSVQQ 900
           ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINV+LGLALDFYSVQQ
Sbjct: 841 ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVILGLALDFYSVQQ 900

Query: 901 TLSSGGAVSAIKARCEMDVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY 960
           TLSSGGAVSAIKARCEM+VDRIEKQFDDCIAFLLRVLSF LNVGHFPHLADLVTRINYSY
Sbjct: 901 TLSSGGAVSAIKARCEMEVDRIEKQFDDCIAFLLRVLSFNLNVGHFPHLADLVTRINYSY 960

Query: 961 FYMSDSGNLRTAPSSETVSSRLGKAFMGRTD 992
           FYMSDSGNLRTAPSSETVSSRLGK FMGRTD
Sbjct: 961 FYMSDSGNLRTAPSSETVSSRLGKTFMGRTD 985

BLAST of Spg016475 vs. ExPASy TrEMBL
Match: A0A6J1C5X9 (Gamma-tubulin complex component OS=Momordica charantia OX=3673 GN=LOC111008244 PE=3 SV=1)

HSP 1 Score: 1781.5 bits (4613), Expect = 0.0e+00
Identity = 903/991 (91.12%), Postives = 935/991 (94.35%), Query Frame = 0

Query: 1   MEQRKNRSLIDSISDIFTNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60
           MEQRK+RSLIDSISDIFTNGIHFAAP+SSLRT ELDLVRGVLQMLQGF GSLFSWD  GK
Sbjct: 1   MEQRKSRSLIDSISDIFTNGIHFAAPVSSLRTCELDLVRGVLQMLQGFPGSLFSWDDIGK 60

Query: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVNSSAKSAPPTLRAFVTSV 120
           KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEV++SAKSA PTLRAFVTSV
Sbjct: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVSTSAKSAAPTLRAFVTSV 120

Query: 121 SSWLKRLRDVALKEEIKINDAGSGTTPTLMGLVGSLSSLCSGAEYLLQIVHKAIPKVYFE 180
           S+WLKRLRDVA  EE+KINDAGS TTPTLMGL GSLSSLCSGAEYLLQIVHKAIPKVYFE
Sbjct: 121 SAWLKRLRDVASSEEMKINDAGSVTTPTLMGLAGSLSSLCSGAEYLLQIVHKAIPKVYFE 180

Query: 181 SSAAITAADLAVHVLDNLYKELDEVCLIQNGQVERTIVEETYQMLLHIFVGSLLPYIEEL 240
           SS AITAA+LAVHVLDNLYK+LDEVCLIQNGQ      EETYQMLLHIFVGSLLPYIEEL
Sbjct: 181 SSDAITAAELAVHVLDNLYKKLDEVCLIQNGQ------EETYQMLLHIFVGSLLPYIEEL 240

Query: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGELSSSIKTEASERES 300
           DSWVFEGILDDP+EELFFYANEAVSVDEH+FWEKSY LRS RLDGELSSSIK EASERES
Sbjct: 241 DSWVFEGILDDPYEELFFYANEAVSVDEHEFWEKSYFLRSPRLDGELSSSIKKEASERES 300

Query: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVCETLSPASEKQNGEEF 360
           ISLSHLLKGKDQYTGGSIACPLFMKD+AKS+VAAGKSLQLIRHVC TLSPASEKQNGEEF
Sbjct: 301 ISLSHLLKGKDQYTGGSIACPLFMKDVAKSMVAAGKSLQLIRHVCATLSPASEKQNGEEF 360

Query: 361 NGSADYGGSLARLSLSELFCVSLAALIGDGDRISRFFWKHDRYKLETDSLLMSRTNRLEV 420
           NG ADYGGSLARLSL+ELFCVSLAALIGDGDRISR+FWKHD+YKL TDSL  S  NRLEV
Sbjct: 361 NGGADYGGSLARLSLAELFCVSLAALIGDGDRISRYFWKHDQYKLGTDSLFKSHMNRLEV 420

Query: 421 ESGISELMCKKKHWYSLLVDASALKGSVCLKSGQKDADKLVGEREKNMTLDMKNCLCSLE 480
           E+GI EL CKKKHW+SLLVDA +LKGS  LKSG K A+KLVGE EK M   M NCLCSLE
Sbjct: 421 ENGIGELTCKKKHWHSLLVDALSLKGSDGLKSGHKVANKLVGESEKKMAFGMTNCLCSLE 480

Query: 481 VFHPENPVMTVCTTILKDNIDVWKRLNLSRCYNLPPLNDESLLKAIFGDEATPFSATKGT 540
            FHPENPV+TVCTTILKDNI+VWKRLNLSRC+NLPPLNDE L KAIFGDE   FSA KGT
Sbjct: 481 SFHPENPVITVCTTILKDNINVWKRLNLSRCFNLPPLNDEGLFKAIFGDEDATFSAIKGT 540

Query: 541 DFTFGFQFDKYEYRHSQNEAKLIETLFPFPTILPAFQDDVHISDLLPFQKNSTLPSRVLS 600
           DFTFGFQFDKYE+ HSQNEAKLIETLFPFPTILPA QDD+H+SDLLPFQKNSTLPSRVL 
Sbjct: 541 DFTFGFQFDKYEHLHSQNEAKLIETLFPFPTILPACQDDLHMSDLLPFQKNSTLPSRVLR 600

Query: 601 WMQNIVPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660
           WMQN+VPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG
Sbjct: 601 WMQNVVPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660

Query: 661 SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVKSNS 720
           SGDLLQHFLTVIF+KLDKGETWDDDFELNTILQES+RNSADGMLLSAPDSLVVSIVKSNS
Sbjct: 661 SGDLLQHFLTVIFDKLDKGETWDDDFELNTILQESMRNSADGMLLSAPDSLVVSIVKSNS 720

Query: 721 LDGDDQSNLVKLPSIPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780
           LDGD+QSNLVKLPS PHKSSAHGFGMDGLDSLKFTYKVSWPLELI NTEAIKKYNQVTGF
Sbjct: 721 LDGDEQSNLVKLPSTPHKSSAHGFGMDGLDSLKFTYKVSWPLELIFNTEAIKKYNQVTGF 780

Query: 781 LLKVKRAKFVLDKTRRWMWKGKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840
           LLKVKRAKFVLDKTRRWMWKGKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR
Sbjct: 781 LLKVKRAKFVLDKTRRWMWKGKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840

Query: 841 ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVVLGLALDFYSVQQ 900
           ELCEGMAAAQSLD VIEVHEAYLL IHRQCFVVPDKLWALIASRINVVLGLALDFYSVQQ
Sbjct: 841 ELCEGMAAAQSLDAVIEVHEAYLLLIHRQCFVVPDKLWALIASRINVVLGLALDFYSVQQ 900

Query: 901 TLSSGGAVSAIKARCEMDVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY 960
            LSSGG VSAIKARCEM+VDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINY+Y
Sbjct: 901 ALSSGGTVSAIKARCEMEVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYNY 960

Query: 961 FYMSDSGNLRTAPSSETVSSRLGKAFMGRTD 992
           FYMSDSGNLRTAPSSET SSRLGKAF+GRTD
Sbjct: 961 FYMSDSGNLRTAPSSETASSRLGKAFVGRTD 985

BLAST of Spg016475 vs. ExPASy TrEMBL
Match: A0A5A7VD97 (Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G00550 PE=3 SV=1)

HSP 1 Score: 1770.7 bits (4585), Expect = 0.0e+00
Identity = 893/991 (90.11%), Postives = 935/991 (94.35%), Query Frame = 0

Query: 1   MEQRKNRSLIDSISDIFTNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60
           MEQRK++SLIDS SDIF NGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK
Sbjct: 1   MEQRKSKSLIDSTSDIFANGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60

Query: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVNSSAKSAPPTLRAFVTSV 120
           KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVN++AKSA PTLRAFVTSV
Sbjct: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVNTAAKSASPTLRAFVTSV 120

Query: 121 SSWLKRLRDVALKEEIKINDAGSGTTPTLMGLVGSLSSLCSGAEYLLQIVHKAIPKVYFE 180
           SSWLKRLRD+ALKEEIK+NDAGSGTTPTLMGL GSLSSLC GAEYLLQIVHKAIPKV+FE
Sbjct: 121 SSWLKRLRDIALKEEIKLNDAGSGTTPTLMGLAGSLSSLCPGAEYLLQIVHKAIPKVFFE 180

Query: 181 SSAAITAADLAVHVLDNLYKELDEVCLIQNGQVERTIVEETYQMLLHIFVGSLLPYIEEL 240
           +SA I  ADLAV+VLDNLYK+LDEVCLIQNGQVE T+VEETYQMLLHIFVGSLLPYIEEL
Sbjct: 181 ASATIAPADLAVYVLDNLYKKLDEVCLIQNGQVETTVVEETYQMLLHIFVGSLLPYIEEL 240

Query: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGELSSSIKTEASERES 300
           DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGE++ SIK E SERES
Sbjct: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGEINLSIKKETSERES 300

Query: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVCETLSPASEKQNGEEF 360
           ISLSHL+KGKDQYTGGSIA PLFMKDIAKSIVAAGKSLQLIRHVCET SPASEKQNGEEF
Sbjct: 301 ISLSHLVKGKDQYTGGSIAIPLFMKDIAKSIVAAGKSLQLIRHVCET-SPASEKQNGEEF 360

Query: 361 NGSADYGGSLARLSLSELFCVSLAALIGDGDRISRFFWKHDRYKLETDSLLMSRTNRLEV 420
           +GS D+GGSLARLSLSELFCVSLAALIGDGDRISR+FWKHD+YK++T S   +RTN  EV
Sbjct: 361 SGSGDFGGSLARLSLSELFCVSLAALIGDGDRISRYFWKHDQYKIKTVSSFKTRTNCSEV 420

Query: 421 ESGISELMCKKKHWYSLLVDASALKGSVCLKSGQKDADKLVGEREKNMTLDMKNCLCSLE 480
           E GI    CK+KHW+SLLVDA   KGSV LKSG KD +K VGE E  MTLD+KNCLCSLE
Sbjct: 421 EKGIDGSTCKRKHWFSLLVDALPPKGSVSLKSGHKDVNKPVGEGENYMTLDIKNCLCSLE 480

Query: 481 VFHPENPVMTVCTTILKDNIDVWKRLNLSRCYNLPPLNDESLLKAIFGDEATPFSATKGT 540
            FHPENPVMTVCT ILKDNI+VWKRLNLSRCYNLPPLNDESLL+AI GDE TPFS TKGT
Sbjct: 481 SFHPENPVMTVCTAILKDNINVWKRLNLSRCYNLPPLNDESLLRAILGDEDTPFSETKGT 540

Query: 541 DFTFGFQFDKYEYRHSQNEAKLIETLFPFPTILPAFQDDVHISDLLPFQKNSTLPSRVLS 600
           DFTFGFQFDK E+ H Q EAKLIETLFPFPT+LPAFQDD+ ISDLLPFQKNSTLPSR+LS
Sbjct: 541 DFTFGFQFDKSEHVHLQKEAKLIETLFPFPTLLPAFQDDLRISDLLPFQKNSTLPSRILS 600

Query: 601 WMQNIVPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660
           WMQNI+PRTMPLTM+IMEECL+VYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG
Sbjct: 601 WMQNIMPRTMPLTMIIMEECLIVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660

Query: 661 SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVKSNS 720
           SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSA GMLLS+P+SLVVSIVK+NS
Sbjct: 661 SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSAVGMLLSSPESLVVSIVKTNS 720

Query: 721 LDGDDQSNLVKLPSIPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780
           LDGD+QSNL KLPS PHKSSAH  GMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF
Sbjct: 721 LDGDEQSNLAKLPSTPHKSSAHFLGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780

Query: 781 LLKVKRAKFVLDKTRRWMWKGKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840
           LLKVKRAKFVLDKTRRWMWKGK T  NN KRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR
Sbjct: 781 LLKVKRAKFVLDKTRRWMWKGKGTPKNNSKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840

Query: 841 ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVVLGLALDFYSVQQ 900
           ELCEGMAAAQSLDGVIEVHEAYLL+IHRQCFVVPDKLWALIASRINV+LGLALDFYSVQQ
Sbjct: 841 ELCEGMAAAQSLDGVIEVHEAYLLTIHRQCFVVPDKLWALIASRINVILGLALDFYSVQQ 900

Query: 901 TLSSGGAVSAIKARCEMDVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY 960
           TLSSGGAVSAIK RCEM+VDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY
Sbjct: 901 TLSSGGAVSAIKLRCEMEVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY 960

Query: 961 FYMSDSGNLRTAPSSETVSSRLGKAFMGRTD 992
           FYMSDSGNLRTAPSSETVSSRLGK+FMGRTD
Sbjct: 961 FYMSDSGNLRTAPSSETVSSRLGKSFMGRTD 990

BLAST of Spg016475 vs. ExPASy TrEMBL
Match: A0A1S3BV48 (Gamma-tubulin complex component OS=Cucumis melo OX=3656 GN=LOC103493512 PE=3 SV=1)

HSP 1 Score: 1770.7 bits (4585), Expect = 0.0e+00
Identity = 893/991 (90.11%), Postives = 935/991 (94.35%), Query Frame = 0

Query: 1   MEQRKNRSLIDSISDIFTNGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60
           MEQRK++SLIDS SDIF NGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK
Sbjct: 1   MEQRKSKSLIDSTSDIFANGIHFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGK 60

Query: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVNSSAKSAPPTLRAFVTSV 120
           KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVN++AKSA PTLRAFVTSV
Sbjct: 61  KFCAKSGIYVSHLSRSSLLAILNQFMYAATCLQLTQLVLQEVNTAAKSASPTLRAFVTSV 120

Query: 121 SSWLKRLRDVALKEEIKINDAGSGTTPTLMGLVGSLSSLCSGAEYLLQIVHKAIPKVYFE 180
           SSWLKRLRD+ALKEEIK+NDAGSGTTPTLMGL GSLSSLC GAEYLLQIVHKAIPKV+FE
Sbjct: 121 SSWLKRLRDIALKEEIKLNDAGSGTTPTLMGLAGSLSSLCPGAEYLLQIVHKAIPKVFFE 180

Query: 181 SSAAITAADLAVHVLDNLYKELDEVCLIQNGQVERTIVEETYQMLLHIFVGSLLPYIEEL 240
           +SA I  ADLAV+VLDNLYK+LDEVCLIQNGQVE T+VEETYQMLLHIFVGSLLPYIEEL
Sbjct: 181 ASATIAPADLAVYVLDNLYKKLDEVCLIQNGQVETTVVEETYQMLLHIFVGSLLPYIEEL 240

Query: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGELSSSIKTEASERES 300
           DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGE++ SIK E SERES
Sbjct: 241 DSWVFEGILDDPFEELFFYANEAVSVDEHDFWEKSYSLRSLRLDGEINLSIKKETSERES 300

Query: 301 ISLSHLLKGKDQYTGGSIACPLFMKDIAKSIVAAGKSLQLIRHVCETLSPASEKQNGEEF 360
           ISLSHL+KGKDQYTGGSIA PLFMKDIAKSIVAAGKSLQLIRHVCET SPASEKQNGEEF
Sbjct: 301 ISLSHLVKGKDQYTGGSIAIPLFMKDIAKSIVAAGKSLQLIRHVCET-SPASEKQNGEEF 360

Query: 361 NGSADYGGSLARLSLSELFCVSLAALIGDGDRISRFFWKHDRYKLETDSLLMSRTNRLEV 420
           +GS D+GGSLARLSLSELFCVSLAALIGDGDRISR+FWKHD+YK++T S   +RTN  EV
Sbjct: 361 SGSGDFGGSLARLSLSELFCVSLAALIGDGDRISRYFWKHDQYKIKTVSSFKTRTNCSEV 420

Query: 421 ESGISELMCKKKHWYSLLVDASALKGSVCLKSGQKDADKLVGEREKNMTLDMKNCLCSLE 480
           E GI    CK+KHW+SLLVDA   KGSV LKSG KD +K VGE E  MTLD+KNCLCSLE
Sbjct: 421 EKGIDGSTCKRKHWFSLLVDALPPKGSVSLKSGHKDVNKPVGEGENYMTLDIKNCLCSLE 480

Query: 481 VFHPENPVMTVCTTILKDNIDVWKRLNLSRCYNLPPLNDESLLKAIFGDEATPFSATKGT 540
            FHPENPVMTVCT ILKDNI+VWKRLNLSRCYNLPPLNDESLL+AI GDE TPFS TKGT
Sbjct: 481 SFHPENPVMTVCTAILKDNINVWKRLNLSRCYNLPPLNDESLLRAILGDEDTPFSETKGT 540

Query: 541 DFTFGFQFDKYEYRHSQNEAKLIETLFPFPTILPAFQDDVHISDLLPFQKNSTLPSRVLS 600
           DFTFGFQFDK E+ H Q EAKLIETLFPFPT+LPAFQDD+ ISDLLPFQKNSTLPSR+LS
Sbjct: 541 DFTFGFQFDKSEHVHLQKEAKLIETLFPFPTLLPAFQDDLRISDLLPFQKNSTLPSRILS 600

Query: 601 WMQNIVPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660
           WMQNI+PRTMPLTM+IMEECL+VYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG
Sbjct: 601 WMQNIMPRTMPLTMIIMEECLIVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLG 660

Query: 661 SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVKSNS 720
           SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSA GMLLS+P+SLVVSIVK+NS
Sbjct: 661 SGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSAVGMLLSSPESLVVSIVKTNS 720

Query: 721 LDGDDQSNLVKLPSIPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780
           LDGD+QSNL KLPS PHKSSAH  GMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF
Sbjct: 721 LDGDEQSNLAKLPSTPHKSSAHFLGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGF 780

Query: 781 LLKVKRAKFVLDKTRRWMWKGKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840
           LLKVKRAKFVLDKTRRWMWKGK T  NN KRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR
Sbjct: 781 LLKVKRAKFVLDKTRRWMWKGKGTPKNNSKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWR 840

Query: 841 ELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVVLGLALDFYSVQQ 900
           ELCEGMAAAQSLDGVIEVHEAYLL+IHRQCFVVPDKLWALIASRINV+LGLALDFYSVQQ
Sbjct: 841 ELCEGMAAAQSLDGVIEVHEAYLLTIHRQCFVVPDKLWALIASRINVILGLALDFYSVQQ 900

Query: 901 TLSSGGAVSAIKARCEMDVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY 960
           TLSSGGAVSAIK RCEM+VDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY
Sbjct: 901 TLSSGGAVSAIKLRCEMEVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSY 960

Query: 961 FYMSDSGNLRTAPSSETVSSRLGKAFMGRTD 992
           FYMSDSGNLRTAPSSETVSSRLGK+FMGRTD
Sbjct: 961 FYMSDSGNLRTAPSSETVSSRLGKSFMGRTD 990

BLAST of Spg016475 vs. TAIR 10
Match: AT1G80260.1 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 1061.6 bits (2744), Expect = 3.8e-310
Identity = 575/982 (58.55%), Postives = 709/982 (72.20%), Query Frame = 0

Query: 22  HFAAPISSLRTSELDLVRGVLQMLQGFSGSLFSWDCSGKKFCAKSGIYVSHLSRSSLLAI 81
           H    + S+  +ELDLVRG+LQ LQG S     WD +G+ F AKS I VSHLS SSL  +
Sbjct: 24  HSELALPSVSVTELDLVRGLLQALQGLSSPFLFWDQTGQTFRAKSHIRVSHLSHSSLHVL 83

Query: 82  LNQFMYAATCLQLTQLVLQEVNSSAKSAPPTLRAFVTSVSSWLKRLRDVALKEEIKINDA 141
           L  F+Y ATCL+L + ++  +N+S +S PPTL AF  SVS+WL+RLRD+ALKEE+ I+++
Sbjct: 84  LAGFLYPATCLKLVESIVAAINTSLRS-PPTLMAFSDSVSAWLERLRDIALKEEVMIDNS 143

Query: 142 GSGTTPTLMGLVGSLSSLCSGAEYLLQIVHKAIPKVYFESSAAITAADLAVHVLDNLYKE 201
               TPTL+GL  SLSSLCSGAEYLLQ+VH AIP  +F+S++ I+AA++AVHVLD LYK+
Sbjct: 144 DITVTPTLLGLTSSLSSLCSGAEYLLQVVHGAIPHTFFDSNSTISAAEIAVHVLDYLYKK 203

Query: 202 LDEVCLIQNGQVERTIVEETYQMLLHIFVGSLLPYIEELDSWVFEGILDDPFEELFFYAN 261
           LDEVCL+Q G+V      E + MLL +F GSLLPYIE LDSW+FEG LDDP EELFF AN
Sbjct: 204 LDEVCLVQGGEV------EGFHMLLQMFAGSLLPYIEGLDSWLFEGTLDDPCEELFFTAN 263

Query: 262 EAVSVDEHDFWEKSYSLRSLRLDGELSSSIKTEASERESIS----LSHLLKGKDQYTGGS 321
           ++VSVD+ +FWEKSY L  +      S S  T  +E++ +S     S L   KD+     
Sbjct: 264 QSVSVDDAEFWEKSYQLMKV----PNSKSNVTSLNEKKVMSGHDANSSLASDKDKEQNTR 323

Query: 322 IACPLFMKDIAKSIVAAGKSLQLIRHVCETLSPASEKQNGEEFNGSADYG---------- 381
           + CPLF+KDI KSIV+AGKSLQL++H+  T   +SE     +F+G   YG          
Sbjct: 324 VLCPLFIKDICKSIVSAGKSLQLMQHIPST---SSENSGKTQFHGRNGYGKSSVGSLLTK 383

Query: 382 ----GSLARLSLSELFCVSLAALIGDGDRISRFFWKHDRYKLETDSLLMSRTNRLEV--- 441
                S A LSLSE+FC++LA LIG GD +SR+ WK +  + E    L S  +   V   
Sbjct: 384 MSSCSSTADLSLSEVFCLTLAGLIGHGDHVSRYLWKDEADEWEISPTLASYISGELVNDM 443

Query: 442 -ESGISELMCKKKHWYSLLVDASALKGSVCLKSGQKDADKLVGEREKNMTLDMKNCLCSL 501
               +  L C ++ WY LLV A   K ++  KS  + A    G ++ N  L  +  L  L
Sbjct: 444 DNKDLPVLTCSERMWYKLLVGAVQEKRAMEAKSELQSACYATGVKDGNSGLTAQKALQGL 503

Query: 502 EVFHPENPVMTVCTTILKDNIDVWKRLNLSRCYNLPPLNDESLLKAIFGDEATPFSATKG 561
             F  EN V++V    L+ N + W  LNLS+ Y LP LNDESLL A+F +     +   G
Sbjct: 504 --FCNENLVVSVSKMDLERNRNAWNVLNLSQNYCLPSLNDESLLSAVFEESGMADAGLSG 563

Query: 562 TDFTFGFQFDKYEYRHSQNEAKLIETLFPFPTILPAFQDDVHISDLLPFQKNSTLPSRVL 621
           T++ FGFQF + EY  SQ++  L+ETLFPFPT+LP+FQ  +H+S+ LPFQKNSTLPSRVL
Sbjct: 564 TNYKFGFQFGRSEYISSQDDTNLLETLFPFPTLLPSFQPKLHLSEFLPFQKNSTLPSRVL 623

Query: 622 SWMQNIVPRTMPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLL 681
           SW+    P    L +VIM+EC  +Y+R+QVDYIGK +LSKLMN+W+LM ELAVLRAIYLL
Sbjct: 624 SWLLKAEPMDTRLPVVIMQECFTIYIRRQVDYIGKVILSKLMNDWKLMHELAVLRAIYLL 683

Query: 682 GSGDLLQHFLTVIFNKLDKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVKSN 741
           GSGDLLQHFLTVIF++L KGE+ +DDFELN ILQESIRNSAD MLLS+PDSLVVSI + +
Sbjct: 684 GSGDLLQHFLTVIFDRLGKGESSNDDFELNIILQESIRNSADAMLLSSPDSLVVSISRED 743

Query: 742 SLDGDDQSNLVKLPSIPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTG 801
             D DD+ +++ L S   KS  + FG+D L+SLKFTYKV WPLELIAN+EAIKKYNQV G
Sbjct: 744 R-DKDDKGDIIPLSS-TRKSRVNSFGIDCLESLKFTYKVPWPLELIANSEAIKKYNQVMG 803

Query: 802 FLLKVKRAKFVLDKTRRWMWKGKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAW 861
           FLLKVKRAK+VLDK RRWMWKGK +     K HWL+EQKLL+FVDAFHQYVMDRVYH+AW
Sbjct: 804 FLLKVKRAKYVLDKARRWMWKGKGSATKIRKHHWLLEQKLLNFVDAFHQYVMDRVYHTAW 863

Query: 862 RELCEGMAAAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVVLGLALDFYSVQ 921
           RELCE M  A SLD VI VHE YLLSI RQCFVV +KLWA+IASRIN++LGLAL+FYS+Q
Sbjct: 864 RELCEAMVKAGSLDEVIYVHETYLLSIQRQCFVVQEKLWAIIASRINMILGLALEFYSIQ 923

Query: 922 QTLSSGGAVSAIKARCEMDVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYS 981
           QTLSSGGAVSAIKARCEM++DRIEKQF+DCIAFLLRVLS KLNVGHFPHLADLVTRINY+
Sbjct: 924 QTLSSGGAVSAIKARCEMEIDRIEKQFEDCIAFLLRVLSSKLNVGHFPHLADLVTRINYN 983

BLAST of Spg016475 vs. TAIR 10
Match: AT1G20570.1 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 982.6 bits (2539), Expect = 2.3e-286
Identity = 546/960 (56.88%), Postives = 672/960 (70.00%), Query Frame = 0

Query: 29  SLRTSELDLVRGVLQMLQGFSGSLFSWDCSGKKFCAKSGIYVSHLSRSSLLAILNQFMYA 88
           S+  +E  LV+G+LQ LQGFS     WD   + F AKS I VSHLS+SSL  +L  F+YA
Sbjct: 36  SVSVNESHLVKGLLQALQGFSSPFIFWDRKEQTFRAKSEIRVSHLSQSSLHVLLAGFLYA 95

Query: 89  ATCLQLTQLVLQEVNSSAKSAPPTLRAFVTSVSSWLKRLRDVALKEEIKINDAGSGTTPT 148
           ATCL+L + ++  +N+S KS PPTL AF  S S WL+   ++AL EE+KIND+    TPT
Sbjct: 96  ATCLKLVESIVSGINASLKS-PPTLMAFSNSASGWLE--ANIALNEEVKINDSNVAVTPT 155

Query: 149 LMGLVGSLSSLCSGAEYLLQIVHKAIPKVYFESSAAITAADLAVHVLDNLYKELDEVCLI 208
           L+GL  SLSSLCS AEYL Q+V  AIP  YFESS+AI+ A++AVHVLD LYK LDEVCL+
Sbjct: 156 LLGLTSSLSSLCSDAEYLFQVVRGAIPHAYFESSSAISTAEIAVHVLDYLYKRLDEVCLV 215

Query: 209 QNGQVERTIVEETYQMLLHIFVGSLLPYIEELDSWVFEGILDDPFEELFFYANEAVSVDE 268
           Q G++   +  E + MLL IF GSLLPY+E LDSW+FEG LDDPFEELFF AN++VSV +
Sbjct: 216 QGGEL---VAVEGFHMLLQIFAGSLLPYVESLDSWLFEGTLDDPFEELFFTANQSVSVSD 275

Query: 269 HDFWEKSYSLRSLRLDGELSSSIKTEASERESISLSHLLKGKDQYTGGSIACPLFMKDIA 328
            +FWEKSY L   R+ G  S+       +  S + S+ +  KD+     + CPLF+KDI 
Sbjct: 276 AEFWEKSYLL--TRVLGPKSNVTSLNQKKGMSGNDSNSVSDKDKEQNNRVLCPLFIKDIC 335

Query: 329 KSIVAAGKSLQLIRHVCETLSPASEKQNGEEFNGSADYG-----------GSLARLSLSE 388
           KSIV+AGKSLQL++H+  T S   EK      NG  + G            S+A LSLSE
Sbjct: 336 KSIVSAGKSLQLMQHIPSTSSENCEKIQYHGRNGFGNSGCGILLAGKNSFRSIADLSLSE 395

Query: 389 LFCVSLAALIGDGDRISRFFWKHDRYKLETDSLLMSRTNRLEVESGISELMCKKKHWYSL 448
           +FC+SLA LIG GD +SR+ WK +  + E    L S  +   V      L   ++ WY L
Sbjct: 396 IFCLSLAGLIGHGDHVSRYLWKDETDEWEISPTLASYISGKLVNGTGDLLTYSERMWYKL 455

Query: 449 LVDASALKGSVCLKSGQKDADKLVGEREKNMTLDMKNCLCSLEVFHPENPVMTVCTTILK 508
           LV A   K S+  KS  +    +   +E+   L  +  L  L  F  EN V++     L+
Sbjct: 456 LVGAVQEKKSIEAKSELQSPCCVTCVKEEKNVLAAEKVLQGL--FCHENLVVSASKMDLE 515

Query: 509 DNIDVWKRLNLSRCYNLPPLNDESLLKAIF-GDEATPFSATKGTDFTFGFQFDKYEYRHS 568
            N + W  LNLS  Y LP LND+SLL A+F G    P     GT++ +GFQF + EY  S
Sbjct: 516 RNKNAWHVLNLSENYCLPSLNDKSLLSAVFEGSGVAP--KFVGTNYKYGFQFGRSEYLSS 575

Query: 569 QNEAKLIETLFPFPTILPAFQDDVHISDLLPFQKNSTLPSRVLSWMQNIVPRTMPLTMVI 628
           Q++ K++ETLFPFPT+LP+FQ  +H+S+ LP+QKNSTLPSRVLSW+    PR   L +VI
Sbjct: 576 QDDTKILETLFPFPTLLPSFQSKLHMSEFLPYQKNSTLPSRVLSWILRTEPRNTLLPVVI 635

Query: 629 MEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLGSGDLLQHFLTVIFNKL 688
           M+EC  + +R+QVD I K + SKLMNEW+LM ELAVLRAIYLLGSGDLLQHFLTVIF++L
Sbjct: 636 MQECFTINIRRQVDNISKVIFSKLMNEWKLMHELAVLRAIYLLGSGDLLQHFLTVIFDRL 695

Query: 689 DKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVKSNSLDGD-DQSNLVKLPSI 748
            KGE+ +DDFELN I+QESIRNSAD MLLS+PD+LVVSI     LD D D    VK  S 
Sbjct: 696 GKGESSNDDFELNIIIQESIRNSADTMLLSSPDALVVSISSEGCLDRDKDDKGDVKSLSS 755

Query: 749 PHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGFLLKVKRAKFVLDKTR 808
           P +SS + + +D L+SLKFTYKV WPLELIAN+EAIKKYNQ       VKRAK+VLDK R
Sbjct: 756 PRESSVNNYAIDCLESLKFTYKVPWPLELIANSEAIKKYNQ-------VKRAKYVLDKAR 815

Query: 809 RWMWKGKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWRELCEGMAAAQSLDGV 868
           R MWKGK +     K H L+EQKLL+FVDAFHQYVMDRVYH+AWRELCE M  A SLD V
Sbjct: 816 RLMWKGKGSATKIRKHHCLLEQKLLNFVDAFHQYVMDRVYHTAWRELCEAMVKAGSLDEV 875

Query: 869 IEVHEAYLLSIHRQCFVVPDKLWALIASRINVVLGLALDFYSVQQTLSSGGAVSAIKARC 928
           I+VHE YLLSI RQCFVV +KLWA+IASRIN++LGLAL+FYS+QQTLSSGGAVSAIKAR 
Sbjct: 876 IDVHETYLLSIQRQCFVVQEKLWAIIASRINMILGLALEFYSIQQTLSSGGAVSAIKARW 935

Query: 929 EMDVDRIEKQFDDCIAFLLRVLSFKLNVGHFPHLADLVTRINYSYFYMSDSGNLRTAPSS 976
           EM++DRIEKQF+DCIAFLLRVL+ K NVGHFPHLADLVTRINY+Y YMSD+G+  TA  S
Sbjct: 936 EMEIDRIEKQFEDCIAFLLRVLTSKKNVGHFPHLADLVTRINYNYHYMSDTGSSMTASGS 976

BLAST of Spg016475 vs. TAIR 10
Match: AT3G43610.1 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 77.0 bits (188), Expect = 9.3e-14
Identity = 78/303 (25.74%), Postives = 128/303 (42.24%), Query Frame = 0

Query: 610  MPLTMVIMEECLVVYLRQQVDYIGKRVLSKLMNEWRLMDELAVLRAIYLLGSGDLLQHFL 669
            +PL  VI ++CL+  +  Q +++ K  +  L   + L + L  LR  + +   D      
Sbjct: 844  LPLDFVI-DKCLLQEIHLQYNFVSKLAIKLLEEGFGLQEHLLALRRYHFMELAD------ 903

Query: 670  TVIFNKLDKGETWDDDFELNTILQESIRNSADGMLLSAPDSLVVSIVKSNSLDGD---DQ 729
                        W D F ++    + +   AD  +      L  SI +S S + D   D+
Sbjct: 904  ------------WADVFVVSLWHHKWLVTEADKRIAEIQGFLESSIQRS-SCERDICKDR 963

Query: 730  SNLVKLPSIPHKSSAHGFGMDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGFLLKVKR 789
              L K     H   +   G+   D L+  Y+V WP+ +I   +A+  Y  V  FL++VK 
Sbjct: 964  IFLYKRQGTMHIPPS-TIGVRSFDFLRLGYRVDWPISIILTCDALTAYADVFSFLVQVKL 1023

Query: 790  AKFVLD------KTRRWMWKGKSTVVNNCKRHWL-----VEQKLLHFVDAFHQYVMDRVY 849
            A +VL       K  R M   K   +   +  WL     +  ++ HFV A  QYV   + 
Sbjct: 1024 AAYVLTDVWCSLKDVRHMMHEKKEKILKQELRWLNILMKLRHQVNHFVTALQQYVHSELS 1083

Query: 850  HSAWRELCEGMA-AAQSLDGVIEVHEAYLLSIHRQCFVVPDKLWALIASRINVVLGLALD 898
            H +W +    +    + +  +  VH AYL    R CF+  +    +I++ I  +L  ALD
Sbjct: 1084 HVSWSKFLHSLKNKVKDMMDLESVHMAYLSEALRICFLSDET--QIISNIIENILQCALD 1123

BLAST of Spg016475 vs. TAIR 10
Match: AT5G06680.1 (spindle pole body component 98 )

HSP 1 Score: 77.0 bits (188), Expect = 9.3e-14
Identity = 60/249 (24.10%), Postives = 103/249 (41.37%), Query Frame = 0

Query: 632 IGKRVLSKLMNEWRLMDELAVLRAIYLLGSGDLLQHFLTVIFNKLDKGETWDDDFELNTI 691
           I K +L  L   ++  +    ++   LLG GD +Q+ + ++  KL +       FEL   
Sbjct: 483 IDKHLLDVLYKRYKFKEHCLAIKRYLLLGQGDFVQYLMDIVGPKLSEPANNISSFELAGF 542

Query: 692 LQESIRNSADGMLLSAPDSLVVSIVKSNSLDGDDQSNLVKLPSIPHKSSAHGFGMDGLDS 751
           L+ +IR S                  +   D  D  + +++  +P     HG G  G D 
Sbjct: 543 LEAAIRAS------------------NAQYDDRDMLDRLRVKMMP-----HGSGDRGWDV 602

Query: 752 LKFTYKVSWPLELIANTEAIKKYNQVTGFLLKVKRAKFVLDKTRRWMWKG---------- 811
               Y+   PL+ +     + KY +V  FL K+KR +  L    + M             
Sbjct: 603 FSLEYEARVPLDTVFTESVLSKYLRVFNFLWKLKRVEHALIGIWKTMKPNCITSNSFVKL 662

Query: 812 ----KSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWRELCEGMAAAQSLDGVIE 867
               K  +++  +R  ++  ++ HFV  F  Y+M  V   +W    + M AA+ LD ++ 
Sbjct: 663 QSSVKLQLLSALRRCQVLWNEMNHFVTNFQYYIMFEVLEVSWSNFSKEMEAAKDLDDLLA 708

BLAST of Spg016475 vs. TAIR 10
Match: AT5G17410.2 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 66.2 bits (160), Expect = 1.6e-10
Identity = 57/260 (21.92%), Postives = 114/260 (43.85%), Query Frame = 0

Query: 630 DYIGKRVLSKLMNEWRLMDELAVLRAIYLLGSGDLLQHFLTVIFNKLDKGETWDDDFELN 689
           ++    +++ + +++ L+  L  ++   LL  GD L HF+ +   +L+K        +L 
Sbjct: 347 EFASIELVNLIKDKYDLVGRLRSIKHYLLLDQGDFLVHFMDIAREELNKKVHEISVEKLQ 406

Query: 690 TILQESIRNSADGMLLSAPDSLV----VSIVKSNSLDGDDQSNLVKLPSIPHKSSAHGFG 749
           ++L  ++R +A        D        S++ +  +  D  SN ++ P            
Sbjct: 407 SLLDLALRTTAAAADPRHEDLTCCVDRASLLTTLGMHKDTDSNSIEDP----------MS 466

Query: 750 MDGLDSLKFTYKVSWPLELIANTEAIKKYNQVTGFLLKVKRAKFVLDKTRRWM------- 809
           + GL++   +YKV WPL ++ + +A+ KY  +  FL   K  +  L     W        
Sbjct: 467 ITGLETFSLSYKVQWPLSIVISKKALSKYQLIFRFLFHCKHVERQL--CGAWQIHQGIRS 526

Query: 810 WKGKSTVVNNCKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWRELCEGMAAAQSLDGVIEV 869
              K T +    R  L+ + +L F+ +   Y+   V    W  + + + + +S+D VI+ 
Sbjct: 527 MNSKGTAI---LRSSLLCRSMLKFISSLLHYLTFEVLEPNWHVMHDRLQSTRSVDEVIQH 586

Query: 870 HEAYLLSIHRQC-FVVPDKL 878
           H+ +L    R C  ++PD L
Sbjct: 587 HDFFLDKCLRGCLLLLPDVL 591