Spg016466 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg016466
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationscaffold9: 45698089 .. 45703250 (+)
RNA-Seq ExpressionSpg016466
SyntenySpg016466
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTCAAAGGAACATGAATTTAGTAAAAGTTATATGATACTGAAGCCAGAGAATGCAAATCTGTTGGATCTGTTTCTGTTCATACTTCCATTTGGATTCAGAAAGAGGAAGTTCATAGATTGCCCAGAGGGCAAAGAAGATTCATACAGAAGCTTTGCAAATCGATGGCTCATCTTCATCTCCATGCTGTTGCAGCTATTTCTTGGCGCCATCGCAGCTCCACTCGCAGCCTTGGACTCCATTCTTCTAAAGCACTTCAACTTCATCTCCTTCAACGGCGGCATTCTTGGGCTTCTGTCTAAGCTGGTCAAAAGTACTTTCAAATTTACCCGCACTTTAATTTCCCTTTTGCTTGCATCCATGGTGATAACAATTTTTAATCCTAAGGTCCCGTTTGATAATCATTTCAGTTTTTTGTTTTTGGTTTTTTTGTTTTTGGTTAAAACAGAAAACCATATATGTTTGGTAACTGTTTTCTGTTTTCTGTTTTTTAAAAATTAGAAAGATGAAAAATTGTTTTAGAGAGAGAAAAATGTGTAGAGAGAAACAAAAATTTAGAGATACAAATATAAATGTTTTTGAGAGATTTTTTTTTTTTTTTTAGAGAAAAGTTAAACATAGAAAATTTAAAAAGGTGAAAGAAAATTTTTAGCGAGAGGATATTAGTTAGACCCGAGAAAAGATATTCGTTAGAGGAAAAATAGAAAAAAAGTTAGAGTAAAAACGATAAATTTTAGATAAAAAATTAAATAAAAAGAGAAAATTTAGAGAGATAGTAAGGTTAAAAGTAAAATATAGGGGCATTTGCATCAATAGCAAAAAAAGAAATCATAATTGCAGGCGTGTCTTCGATTTATTGGGTTTGCAGATATAGCAAAAAAAGTTTGGCCCGGCCCAAACAATAACGCAAAAAGACTAAAATAACCTCAGCTCGCCATCCACTGATATACCAAATATCTAATATATTAAACCCTAAAAATATACTAGATATTTGGGATATCAATCAACTGATATACTAAATATATAGTATAGCAGATGTATATTTTCGTGAATATAATATACTTCATTTATGAAGTATACACATGAGCTTTCATTAGTTTTAGATATCTTTGATATACTTTATCAATGAAGAATTAGATATATTAGCTTTATTTTGGGACAATATGTGTGGTAGAGGATTTAAACCTCTAACCTCTTTGTTGAATGTGTATATATCAATGATTTTGTTTAATAACAAATCTATCGTTGATGTATTTAGCTACTCATAATCAAGTATATCAATGTTATTAATTATTTGAGTAGTATAAAAAATGAGGTAAGTTAAAAAAAAAATACAAATGAGGTATATCAATGGTGTATCAACATTCATAACAGGTATAGAAGACACATTTATTTTATAAAAGACAAACATTTTATTTAAAATATGTGAGTAACACCACATAATTCAATAAAGAGAAAAAAAAATATAACAAATATAAGTAAAGAAAGTAACATATCAAGTATATCATTGGTATATCAAATGTTATGCTTATCAAATCAAGTATATATTAGGTATATCATTGTATTCATTCGAGTATATCAAATTTGGTATACTTTATATTTCGTATATCAGTAAATATAAAATATATCAGATATACTACTAATATACCTATCGATATACCAACAAGGGTAAAATTGGAATAATAAAAAAAACGGAAATCAGATTTTTAAATTTTTGCTATGTTTGCAAATCTAAAAAATCATAGCTAAATTTGCAAATATCCTAATATTTTTTGCTACCCATTGCGATTCCCCTAAAATATATGCCAAAGAAATAACGATAAATTAGAAAAAAAAATTGTAGCGATAGAAAATTTTGGAGATTTTTTTTTTCAAAAGAAAAATTAGAATATGTTTATTAAGGAGAGAAAAGTTATTGATAAGTTTTTAGAAATAGATAAGTTATAGAGAAAAAAAAAAATTTTGAGAGAAAATTTTAGGGAGAGAAAAAGAAAGTTTATATAGAGAGAAGAACGTTTTAGAGAGAAAAAAATAGAAAAATGAATTCATTGAAGAGAGATGGGTAAAAGAGAAGAAAAATAAGCAAAATGAGTTTTTGAAAAACATAATTTTTTTTTTAAAAACTACTTTTTGAAATTTGTTTATAATTTCAAAAAAACGTTTTTGAAAAACAAAAAACAAAAAACAGAAATGGTTATCAAACACGCCTGTTTTTTTAAAATGAAAAACAAGAACAAAAAATGAAATGGTTTTCAAACGGACCTAATAGTTTTGTTTTTGTGTGCTTAGGAAAGCCTTTTGTGCAGCCTGATGAAAATTCCCCGGACTACAGATCGGTGGTCGGATTTACAGATTGGAGAAAAGATTTGGACTCATCCATAAAACCCGACGATACTTTCAGATATTATGGTGCCCTAACTATGATGGCTGCCAAAGTCTCCTATGAATTCCTCCCATTTATCCAATCTGTCGTAAATGACCATTGGAAGGTAAATATACTATTCAGGTCCTCTATTTTTAGTTTTGGTTAGGACATCATATCATAATATCTGAAAGTGTCGTCGTAATATATGATCTGATTGGTGTTTTTTTTTTTTTTTTTGAGTACATCAACATTTGGGGTGGGGAGATTCAAACCTATGACCTCATGGTCGAGGGTACATGCCAATTACCGCTGAACCATGCTCGTTTTGGCTGATCTGATTGGTGTTAAAATATAAGTTTATATCATATTGATTAAGGTTGTATTGTACTTGAACAAAAAAAACAGGGATGATAGTTGAACAACGACGGTGGGATCAAAATGATTATTTTTTTGAATAATTTTAATTAATTATTGGATAGCAAAATAAAATTTAAACCAAAATTTTAAATGTATATCCATCAATTAATAGTTGTATATGATTGTGACCATAGTTTTCGTGATCTTTGAATACTCCAAATGGTGACGATGTTTTCTTCTTCATGTTGCAGATGAAGATGCTCCACTACTACGCCTTTTGGAATGGTAAAGACTGACATTTTTTTTTTTTTTGAAAAAAAAAAGACTTTGACATCTTAATTAACGGTCTTAATTCCTATACTTTTAAATTGATTTCTTTGATTTAATCAAATCTCAATATTATTACAATATACAATTTTTATGTGACAATGAGTTTATGTTTTCTAATTTACTATGTTACGTACTTGATTTTATAATGATTTAATGTTGAGTAAAAAAATAGAAGTACTGATGAGGGTGAGACTTTTGTGTGAGTTTAACCCTGTAAAAATAATTTGTGAAGTTTAACAAAATCATAGGGACATATTAATAATTACAAAGTTTTTCAACTTTGTAATTCTTTTTCTTTTGCCAACAAGAGCATAGCTCAATTGGATTTATGGATAACTATGAGGTACGTGGTTCGAATCCCCACCCCCATATTATAAAAAAAATATTTTATTTTTTTTTAAAATTGTAATTGTTTGAGCCCGTTTGATCATATTCATCTTACTCTACTTTTTAAATACGACCTCAAAATCCAAGTCAAATCTTAAAAGCATAAACAAGCAATTTTTTTAAAACTTGATTTTGTTGTTTAAAAAAAAAACTTGATTTAATTTTGTTTTTGAAATTTTTCTAAGAATACTAACGTTTCATAGTTAAGAAAGTATAATATAAGGAATTAACCATCAAAATTTTAAAAAATAGAAAAGAAAACAAAATGATATACAAAACGGGGGATTCATAACCATTTTTAGTTTTAAAAATTTCTAACATGTTTATAAAACTGTAAATAAATTATCAAAACGAAACATGGATATTTTGTTGCTCATGCAAAAGTTTTAGGATCGTGTAAAAGTAAACCCTAATTTATTTCAAAAATCCTTTCAGACTTTCAGAATAAAACTACGACACAAGCCTTCATATTTCAGAACATAGCAACAGACTCAAACATAACAGTGATAGCATTCCGAGGCACCTCTCCCCTCGACGCCTACGACTGGCAAGTGGATGCGGATTTTTCTTGGTACGAAATTGAAGGCATTGGTCGCATCCACAGCGGCTTCATGAAAGCCCTTGGCCTACAAAAGGCCACAGGCTGGCCCAAGAACCTCCCTCCGAGCCCTGACAATCGCCAATTTGCCTACTACACCCTTCGCCAACAGCTGAGAGACATTGCCAAAGCCAACGACAAGGCGAGGTTCATCATCACTGGCCACAGCCTGGGCGGAGCCCTCGCAACCCTCTTTGTCACTCTGCTCTCCTTCCACCAAGAATCGACTCTATTGGAGAAGTTGCAGGCTGTTTACACCTATGGCCAGCCCAGAGTCGGAGACCAGCAATTTGCAGAGTTCATGGTAAATACTATCCACAAATATGGCTTCAAATACTACAGATACGTTTACTCTAATGACTTGGTGCCTAGGATTCCTGCTGATGGTATTGTTTTCGAATACAAACACTTTGGTGGATGTGTTTTCTTCAACAGCCTCTACAAAGGAAAAGTAAGAACAAAACTCCCCTTTTCTCTCTTCTCTTCTTTGTCTTTAAATGAAATATATACTCATTTATAATGTATTTGGAATAGCTTTGAAAAAAAAATGTTTTTTTAAATAATATGATCAGCTTTTCTATAACCACACATTTGAAAAAATGGCTAGTTATAAAACCCCTTTTTAAGAGGTGAGAGAGGGTCCATAACATTCACCAAACCTGGAGATAAAACACTGTAGCAGAAGCCATTTTTTAATTTTTAGCATCCACTTCTTTTTTCAAAGATTTTTTTTTATTTTTTTTTTTCTAAAAGTAAGATAAAAAATATTTTTAAATATTAATTTTTTTTTTATTGGTGTAGATTCTGAGGGAGCAACCAAATAAGAACTATTTCTCATTGGTTTGGTTGATACCCAAGTATTTATTTGCATGGTGGGAGCTGATAAGGAGTTTCATAATCCCAATAGTGAAGGGTTCAGTTTACTATGAGGGTTTGCTAATGAAAGGGGTGAGGGTGTTTGGATTGATTATTCCAGGAGCCGCCGCCCACTTTCCTCAGGACTATGTTAATTCGACCCGTTTGGGACTCTTACAGATACCCGATGTGATTGAAGACCCAATTCTTGGAAACTACGTCGAAGCCGACAGCCAAAACCTATGGATATGGAGAAGAAGAAGCTGA

mRNA sequence

ATGATTTCAAAGGAACATGAATTTAGTAAAAGTTATATGATACTGAAGCCAGAGAATGCAAATCTGTTGGATCTGTTTCTGTTCATACTTCCATTTGGATTCAGAAAGAGGAAGTTCATAGATTGCCCAGAGGGCAAAGAAGATTCATACAGAAGCTTTGCAAATCGATGGCTCATCTTCATCTCCATGCTGTTGCAGCTATTTCTTGGCGCCATCGCAGCTCCACTCGCAGCCTTGGACTCCATTCTTCTAAAGCACTTCAACTTCATCTCCTTCAACGGCGGCATTCTTGGGCTTCTGTCTAAGCTGGTCAAAAGAAAGCCTTTTGTGCAGCCTGATGAAAATTCCCCGGACTACAGATCGGTGGTCGGATTTACAGATTGGAGAAAAGATTTGGACTCATCCATAAAACCCGACGATACTTTCAGATATTATGGTGCCCTAACTATGATGGCTGCCAAAGTCTCCTATGAATTCCTCCCATTTATCCAATCTGTCGTAAATGACCATTGGAAGATGAAGATGCTCCACTACTACGCCTTTTGGAATGACTTTCAGAATAAAACTACGACACAAGCCTTCATATTTCAGAACATAGCAACAGACTCAAACATAACAGTGATAGCATTCCGAGGCACCTCTCCCCTCGACGCCTACGACTGGCAAGTGGATGCGGATTTTTCTTGGTACGAAATTGAAGGCATTGGTCGCATCCACAGCGGCTTCATGAAAGCCCTTGGCCTACAAAAGGCCACAGGCTGGCCCAAGAACCTCCCTCCGAGCCCTGACAATCGCCAATTTGCCTACTACACCCTTCGCCAACAGCTGAGAGACATTGCCAAAGCCAACGACAAGGCGAGGTTCATCATCACTGGCCACAGCCTGGGCGGAGCCCTCGCAACCCTCTTTGTCACTCTGCTCTCCTTCCACCAAGAATCGACTCTATTGGAGAAGTTGCAGGCTGTTTACACCTATGGCCAGCCCAGAGTCGGAGACCAGCAATTTGCAGAGTTCATGGTAAATACTATCCACAAATATGGCTTCAAATACTACAGATACGTTTACTCTAATGACTTGGTGCCTAGGATTCCTGCTGATGGTATTGTTTTCGAATACAAACACTTTGGTGGATGTGTTTTCTTCAACAGCCTCTACAAAGGAAAAATTCTGAGGGAGCAACCAAATAAGAACTATTTCTCATTGGTTTGGTTGATACCCAAGTATTTATTTGCATGGTGGGAGCTGATAAGGAGTTTCATAATCCCAATAGTGAAGGGTTCAGTTTACTATGAGGGTTTGCTAATGAAAGGGGTGAGGGTGTTTGGATTGATTATTCCAGGAGCCGCCGCCCACTTTCCTCAGGACTATGTTAATTCGACCCGTTTGGGACTCTTACAGATACCCGATGTGATTGAAGACCCAATTCTTGGAAACTACGTCGAAGCCGACAGCCAAAACCTATGGATATGGAGAAGAAGAAGCTGA

Coding sequence (CDS)

ATGATTTCAAAGGAACATGAATTTAGTAAAAGTTATATGATACTGAAGCCAGAGAATGCAAATCTGTTGGATCTGTTTCTGTTCATACTTCCATTTGGATTCAGAAAGAGGAAGTTCATAGATTGCCCAGAGGGCAAAGAAGATTCATACAGAAGCTTTGCAAATCGATGGCTCATCTTCATCTCCATGCTGTTGCAGCTATTTCTTGGCGCCATCGCAGCTCCACTCGCAGCCTTGGACTCCATTCTTCTAAAGCACTTCAACTTCATCTCCTTCAACGGCGGCATTCTTGGGCTTCTGTCTAAGCTGGTCAAAAGAAAGCCTTTTGTGCAGCCTGATGAAAATTCCCCGGACTACAGATCGGTGGTCGGATTTACAGATTGGAGAAAAGATTTGGACTCATCCATAAAACCCGACGATACTTTCAGATATTATGGTGCCCTAACTATGATGGCTGCCAAAGTCTCCTATGAATTCCTCCCATTTATCCAATCTGTCGTAAATGACCATTGGAAGATGAAGATGCTCCACTACTACGCCTTTTGGAATGACTTTCAGAATAAAACTACGACACAAGCCTTCATATTTCAGAACATAGCAACAGACTCAAACATAACAGTGATAGCATTCCGAGGCACCTCTCCCCTCGACGCCTACGACTGGCAAGTGGATGCGGATTTTTCTTGGTACGAAATTGAAGGCATTGGTCGCATCCACAGCGGCTTCATGAAAGCCCTTGGCCTACAAAAGGCCACAGGCTGGCCCAAGAACCTCCCTCCGAGCCCTGACAATCGCCAATTTGCCTACTACACCCTTCGCCAACAGCTGAGAGACATTGCCAAAGCCAACGACAAGGCGAGGTTCATCATCACTGGCCACAGCCTGGGCGGAGCCCTCGCAACCCTCTTTGTCACTCTGCTCTCCTTCCACCAAGAATCGACTCTATTGGAGAAGTTGCAGGCTGTTTACACCTATGGCCAGCCCAGAGTCGGAGACCAGCAATTTGCAGAGTTCATGGTAAATACTATCCACAAATATGGCTTCAAATACTACAGATACGTTTACTCTAATGACTTGGTGCCTAGGATTCCTGCTGATGGTATTGTTTTCGAATACAAACACTTTGGTGGATGTGTTTTCTTCAACAGCCTCTACAAAGGAAAAATTCTGAGGGAGCAACCAAATAAGAACTATTTCTCATTGGTTTGGTTGATACCCAAGTATTTATTTGCATGGTGGGAGCTGATAAGGAGTTTCATAATCCCAATAGTGAAGGGTTCAGTTTACTATGAGGGTTTGCTAATGAAAGGGGTGAGGGTGTTTGGATTGATTATTCCAGGAGCCGCCGCCCACTTTCCTCAGGACTATGTTAATTCGACCCGTTTGGGACTCTTACAGATACCCGATGTGATTGAAGACCCAATTCTTGGAAACTACGTCGAAGCCGACAGCCAAAACCTATGGATATGGAGAAGAAGAAGCTGA

Protein sequence

MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIFISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYRSVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYVEADSQNLWIWRRRS
Homology
BLAST of Spg016466 vs. NCBI nr
Match: XP_038899591.1 (triacylglycerol lipase OBL1-like [Benincasa hispida])

HSP 1 Score: 774.2 bits (1998), Expect = 6.6e-220
Identity = 364/479 (75.99%), Postives = 419/479 (87.47%), Query Frame = 0

Query: 5   EHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIFISML 64
           E EFSK+Y+ILKPENANLLDLFLF+LPFG RKRKFIDCP GKED +++F +RWLIFISML
Sbjct: 5   ESEFSKNYVILKPENANLLDLFLFLLPFGLRKRKFIDCPLGKEDPFKNFRDRWLIFISML 64

Query: 65  LQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYRSVVG 124
           LQ  L  IA PLA LDS LLK  NFISFNGG+ G+LSK++K K F++ +E+SP+Y SVVG
Sbjct: 65  LQFILAIIATPLAKLDSFLLKLLNFISFNGGVSGILSKILKGKSFLEVNEDSPEYTSVVG 124

Query: 125 FTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWND 184
           F DWR+DLDSSIKPDDTFRYY ALT+MAAKVSYE  PF+QSVVND WKMK+L Y+ FWND
Sbjct: 125 FADWRRDLDSSIKPDDTFRYYSALTVMAAKVSYESKPFVQSVVNDRWKMKLLGYFNFWND 184

Query: 185 FQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMK 244
           FQ K TTQAF+F+N A D N+ V+AFRGTS LD+YDWQVDADFSWYEIEG+GRIHSGFMK
Sbjct: 185 FQGKATTQAFMFENTAIDPNVIVVAFRGTSLLDSYDWQVDADFSWYEIEGVGRIHSGFMK 244

Query: 245 ALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFV 304
           ALGLQKATGWPK LP S   ++FAYYT+RQ+LRDIAK NDKARFIITGHSLGGALATLFV
Sbjct: 245 ALGLQKATGWPKELPKS-QLQEFAYYTIRQKLRDIAKTNDKARFIITGHSLGGALATLFV 304

Query: 305 TLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIP 364
           T+LS H EST+LEKL+ +YTYGQPRVGDQQFA+FMVNT+ KYGFKY+RYVYS+DLVPR+P
Sbjct: 305 TVLSLHDESTILEKLEGIYTYGQPRVGDQQFAKFMVNTVQKYGFKYHRYVYSSDLVPRLP 364

Query: 365 ADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPIV 424
           +D I+F+YKHFG CVFFN+LY+G+I++EQPNKNYFSLVW+IPKYL AWWELIRS IIP+V
Sbjct: 365 SDAILFKYKHFGRCVFFNTLYRGRIVKEQPNKNYFSLVWVIPKYLSAWWELIRSLIIPLV 424

Query: 425 KGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYVEAD 484
           KG  YYE LLMKG RV GL IPG AAHFP +YVNSTRLG L +PD +EDPILG+ +E D
Sbjct: 425 KGFDYYESLLMKGGRVVGLFIPGLAAHFPLNYVNSTRLGKLNVPDKVEDPILGDDIEDD 482

BLAST of Spg016466 vs. NCBI nr
Match: XP_008452563.1 (PREDICTED: uncharacterized protein LOC103493549 [Cucumis melo])

HSP 1 Score: 754.6 bits (1947), Expect = 5.4e-214
Identity = 351/483 (72.67%), Postives = 412/483 (85.30%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M+  E+EFSK+Y+ILKPENANLLDLFLF+LPFG RK+KFIDCP GKED + +FA+RWLIF
Sbjct: 1   MVLMENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFADRWLIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
           +SMLLQ  L  IA PLA LD+ LLK FNFISFNGG+LG+LSK+++ K FV+  E+S +Y 
Sbjct: 61  VSMLLQFILALIATPLAKLDAFLLKLFNFISFNGGLLGILSKILRGKGFVKVKEDSSEYT 120

Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
           SVVGF DWR+DLDSSIK + +FRYY ALT+MA K+SYE  PF+QSVVND WKMK+L Y+ 
Sbjct: 121 SVVGFADWRRDLDSSIKSEKSFRYYSALTVMATKISYESKPFVQSVVNDRWKMKLLGYFN 180

Query: 181 FWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHS 240
           FWNDFQ K TTQAF F+N A D N+ ++AFRGTSPLDAYDWQVD D SWYEIEG+GRIHS
Sbjct: 181 FWNDFQGKATTQAFTFENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHS 240

Query: 241 GFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALA 300
           GFMKALGLQKATGWPK LP +    +FAYYTLR+QLRDIAKAN+KARFI TGHSLGGALA
Sbjct: 241 GFMKALGLQKATGWPKELPKT-QTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALA 300

Query: 301 TLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLV 360
           TLFVT+LS H EST+LEKL +VYTYGQPRVGDQQFA+FMVN + KYGFKY+RYVYS DLV
Sbjct: 301 TLFVTVLSLHDESTILEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLV 360

Query: 361 PRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFI 420
           PR+P+D ++F+YKHFG CV+FNSLYKG+I++EQPN+NYFSL+W+IPKYL AWWELIRSFI
Sbjct: 361 PRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRSFI 420

Query: 421 IPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYV 480
           IP VKG  YYE LLMKG R+ GL+IPG +AHFP +YVN TRLG L +PD +EDP LG  +
Sbjct: 421 IPFVKGFDYYESLLMKGARLVGLLIPGLSAHFPLNYVNVTRLGKLNVPDEVEDPFLGYEI 480

Query: 481 EAD 484
           E D
Sbjct: 481 EDD 482

BLAST of Spg016466 vs. NCBI nr
Match: XP_011654113.1 (uncharacterized protein LOC101203640 [Cucumis sativus] >KGN55199.1 hypothetical protein Csa_012910 [Cucumis sativus])

HSP 1 Score: 748.4 bits (1931), Expect = 3.9e-212
Identity = 350/483 (72.46%), Postives = 410/483 (84.89%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M+  E+EFSK+Y+ILKPENANLLDLFLF+LPFG RK+KFIDCP GKED + +F +RWLI 
Sbjct: 1   MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
           +SMLLQ  L  IA PLA LD+ LLK FNFISFNGG LG+LSK+++ K FV+ +E+S +Y 
Sbjct: 61  VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120

Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
           SVVGF DWR+DLDSSIKP+ +FRYY ALT MA K+SYE  PF+QSVVND WKMK+L Y+ 
Sbjct: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180

Query: 181 FWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHS 240
           FWNDFQ +TTTQAF+F+N A D N+T++AFRGTSPLDAYDWQVD DFSWYEIE +G IHS
Sbjct: 181 FWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHS 240

Query: 241 GFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALA 300
           GFMKALGLQKATGWPK LP +    +FAYYTLR+QLRDIAKAN+KARFI TGHSLGGALA
Sbjct: 241 GFMKALGLQKATGWPKELPKT-QTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALA 300

Query: 301 TLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLV 360
           TLFVT+L  H EST+LEKL +VYTYGQPRVGDQQFA+FM+N I KYGFKY+RYVYS DLV
Sbjct: 301 TLFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLV 360

Query: 361 PRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFI 420
           PR+P+D ++F+YKHFG CV+FNSLYKG+I++EQPNKNYFSL+W+IPKYL AWWELIRSFI
Sbjct: 361 PRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFI 420

Query: 421 IPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYV 480
           IP VKG  YYE LLMKG R+ GL+IPG  AHFP +YVN TRLG L +PD +EDPIL   +
Sbjct: 421 IPFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEI 480

Query: 481 EAD 484
           E D
Sbjct: 481 EDD 482

BLAST of Spg016466 vs. NCBI nr
Match: XP_022940183.1 (uncharacterized protein LOC111445886 [Cucurbita moschata])

HSP 1 Score: 741.5 bits (1913), Expect = 4.7e-210
Identity = 353/484 (72.93%), Postives = 408/484 (84.30%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M+ KE EFSK+YMILKP +A LLDLFLF+LPFG RK KFIDCPEG EDSY +F +RW IF
Sbjct: 1   MVWKECEFSKNYMILKPNDAKLLDLFLFLLPFGLRKTKFIDCPEGNEDSYNNFTDRWSIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
           IS+LLQ+ L A A PLA LDS L+  FNFISFNGGILGLL+K++K K  V+P+E+S DY 
Sbjct: 61  ISVLLQIILAAAATPLAKLDSFLVNLFNFISFNGGILGLLAKILKGKGLVKPNEDSSDYT 120

Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
           SVVGF DWR DLDSSI   DTFRYYG LT+MAAK++YE  PF+QSVVND WKMK+L ++ 
Sbjct: 121 SVVGFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFN 180

Query: 181 FWNDFQNKTTTQAFIFQNIATDS-NITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIH 240
           FWNDFQ K TTQAF+FQN ATD  NI VIAFRGTSPLD YDWQVDADFSWY+IEG+GRIH
Sbjct: 181 FWNDFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTYDWQVDADFSWYDIEGVGRIH 240

Query: 241 SGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGAL 300
           SGFMKALGLQKATGWPK L  +     FAYYTLRQ+LRDIAKAN KARFIITGHSLGGAL
Sbjct: 241 SGFMKALGLQKATGWPKEL--TKPQHDFAYYTLRQKLRDIAKANAKARFIITGHSLGGAL 300

Query: 301 ATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDL 360
           ATLFVTLLS H E+ +LEKLQ VYTYGQPRVGD++FAEFMVN++ +YGFKYYRYVYS+DL
Sbjct: 301 ATLFVTLLSLHDETPILEKLQGVYTYGQPRVGDRRFAEFMVNSVQQYGFKYYRYVYSSDL 360

Query: 361 VPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSF 420
           VPRIP+DG++F+YKHFG  ++FN+LYKG+I++ QPNKNYFSL+W+IPKYL AWWEL+RSF
Sbjct: 361 VPRIPSDGVIFKYKHFGRSIYFNTLYKGRIVKGQPNKNYFSLLWVIPKYLSAWWELVRSF 420

Query: 421 IIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNY 480
           I P+V G  YYE LLM   R+ GLIIPG AAHFP +YVNSTRLG L+ PD +EDPIL + 
Sbjct: 421 ITPLVWGFDYYESLLMSAARIIGLIIPGLAAHFPLNYVNSTRLGKLKAPDEVEDPILEDD 480

Query: 481 VEAD 484
           +E D
Sbjct: 481 IEGD 482

BLAST of Spg016466 vs. NCBI nr
Match: XP_022139642.1 (uncharacterized protein LOC111010491 [Momordica charantia])

HSP 1 Score: 735.3 bits (1897), Expect = 3.4e-208
Identity = 346/470 (73.62%), Postives = 406/470 (86.38%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKR-KFIDCPEGKEDSY-RSFANRWL 60
           M SKEHEFSK+Y+ILKP+NAN++DLFLF+LPFGFRKR KFIDCPEGKEDSY  SF NRWL
Sbjct: 1   MASKEHEFSKNYLILKPQNANIVDLFLFLLPFGFRKRTKFIDCPEGKEDSYTSSFTNRWL 60

Query: 61  IFISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPD 120
           IFIS++LQ+ L AIA PLA LD+ LL  FNFISFNGGILGLLS+++K +  V+PDENS +
Sbjct: 61  IFISIVLQIILLAIATPLAKLDAFLLNVFNFISFNGGILGLLSRILKGESLVKPDENSAE 120

Query: 121 YRSVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHY 180
           Y SVVGF DWR+DLD SIK ++ FRYYG LT+MAA++SYE  PF+ SVVND WKMK+L +
Sbjct: 121 YTSVVGFADWRRDLDKSIKAEEIFRYYGGLTVMAAQLSYESKPFVLSVVNDRWKMKLLGH 180

Query: 181 YAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRI 240
           + FWNDFQ K TTQAF+FQN A D N+TV+AFRGTSPLDAYDWQVDADFSWY+IEG+GRI
Sbjct: 181 FNFWNDFQGKATTQAFMFQNTAADPNVTVVAFRGTSPLDAYDWQVDADFSWYDIEGVGRI 240

Query: 241 HSGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGA 300
           HSGFMKALGLQKATGWP+ L  S     FAYYTLRQ+LRDIAK+ND A+FIITGHSLGGA
Sbjct: 241 HSGFMKALGLQKATGWPEELVKS--KHHFAYYTLRQKLRDIAKSNDNAKFIITGHSLGGA 300

Query: 301 LATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSND 360
           LA LFVT+LS H++S +LEKLQAVYTY QPRVGD++FAEFMVNT+ KYGFKY+RYVYS+D
Sbjct: 301 LAALFVTVLSLHRDSAVLEKLQAVYTYSQPRVGDRRFAEFMVNTVKKYGFKYHRYVYSSD 360

Query: 361 LVPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRS 420
           LVPR+P DG++F+YKHFG C++FNSLYKG+I++E+PNKNYFSL+W++PKYL AWWELIRS
Sbjct: 361 LVPRVPFDGVIFKYKHFGRCIYFNSLYKGRIVKEEPNKNYFSLIWVMPKYLNAWWELIRS 420

Query: 421 FIIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIP 469
           FI+P+VKG  Y+E LLMKG RV GL IPG AAH P DYVNS RLG    P
Sbjct: 421 FIVPLVKGFDYHESLLMKGARVVGLFIPGLAAHIPTDYVNSIRLGKFNPP 468

BLAST of Spg016466 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 6.4e-85
Identity = 178/479 (37.16%), Postives = 257/479 (53.65%), Query Frame = 0

Query: 55  NRWLIFISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDE 114
           +RW+IF+S++++  +     P+     ++    N  S NG  LGLL  ++  K  V P  
Sbjct: 85  HRWVIFVSIIVRKLIAIFGKPMEWTGYLVEFFLNLFSLNGNFLGLLYNILHGK-VVMPHR 144

Query: 115 NSPDYRSVVGFTDWR------KDLDSSIKPDDTFRYYGA-------LTMMAAKVSYEFLP 174
            S  + S +G  D R      + L   I   D ++  G        L MMA+K++YE   
Sbjct: 145 GSETFISAIGHLDGRINLYKSETLTKEIGEPDFWQKIGIGHRDLMDLCMMASKLAYENEK 204

Query: 175 FIQSVVNDHWKMKMLHYYAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDW 234
            +++VVN HWKM  + +Y  WNDF+ + +TQ F+  +   D+N+ +++FRGT P DA DW
Sbjct: 205 VVRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDANLILVSFRGTEPFDADDW 264

Query: 235 QVDADFSWYEIEGIGRIHSGFMKALGLQKATG--------WPKNL------------PPS 294
             D D+SWYEI  +G++H GF++ALGL   T         +  NL            PPS
Sbjct: 265 ITDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFVNNLKFANLENVHATIPPS 324

Query: 295 PDNR-------------------------------------QFAYYTLRQQLRDIAKAND 354
             ++                                     + AYY +R +L+ + K + 
Sbjct: 325 ESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERTAYYVVRSKLKRLLKEHK 384

Query: 355 KARFIITGHSLGGALATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIH 414
            A+F++TGHSLGGALA LF  +L  H+E  ++E+L  +YTYGQPRVG++Q   FM   + 
Sbjct: 385 NAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRVGNRQLGRFMEAHLE 444

Query: 415 KYGFKYYRYVYSNDLVPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWL 464
               KY+R VY NDLVPR+P D   F +KHFG C ++NSLY  + + E+PN NYF + +L
Sbjct: 445 HPVPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNSLYIEQNINEEPNPNYFGMRFL 504

BLAST of Spg016466 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.6e-83
Identity = 171/492 (34.76%), Postives = 273/492 (55.49%), Query Frame = 0

Query: 11  SYMILKPENANLLDLFLF-ILPFGFRKRKFIDCPEGKEDS-----YRSFANRWLIFISML 70
           +Y+I++P     +DLF + +      K KF++ P+ +E S       +  +RW+I +S+L
Sbjct: 18  NYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDHRWVIVVSIL 77

Query: 71  LQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYRSVVG 130
           ++  +  +  P+     ++    N  S NGG  GLL +L++ K  V P+  S  + S +G
Sbjct: 78  VRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAK-VVIPERGSVTFVSTIG 137

Query: 131 FTDWRKDL-----------------DSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVV 190
             D R  L                    +K +   R    L +MA+K++YE    +++VV
Sbjct: 138 QLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYENAKVVENVV 197

Query: 191 NDHWKMKMLHYYAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADF 250
           + HWKM ++ +   WND+Q + +TQ F+F +   D+N+ VI+FRGT P DA DW  D D+
Sbjct: 198 DLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDADDWGTDFDY 257

Query: 251 SWYEIEGIGRIHSGFMKALGL---QKATGWPKNLPPSPDNRQ-------------FAYYT 310
           SWYE+  +G++H GF++A+GL      T +  NL     + +              AYY 
Sbjct: 258 SWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDMVERSAYYA 317

Query: 311 LRQQLRDIAKANDKARFIITGHSLGGALATLFVTLLSFHQESTLLEKLQAVYTYGQPRVG 370
           +R  L+ +   ++ ARF++TGHSLGGALA LF TLL  ++E+ ++++L  VYT+GQPR+G
Sbjct: 318 VRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFGQPRIG 377

Query: 371 DQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIPADGIVFEYKHFGGCVFFNSLYKGKILR 430
           +++   FM   +++   +Y+R VY ND+VPR+P D   F YKHFG C+F++S Y      
Sbjct: 378 NREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFYDSFYNETKAE 437

Query: 431 EQPNKNYFSLVWLIPKYLFAWWELIRSFIIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAH 464
           ++P+ N + L + I  ++ A WEL+R   +    G  Y EG      R+ GL+IPG + H
Sbjct: 438 DEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYKEGWFRILFRLMGLVIPGLSDH 497

BLAST of Spg016466 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 5.4e-76
Identity = 176/506 (34.78%), Postives = 263/506 (51.98%), Query Frame = 0

Query: 9   SKSYMILKPENANLLDLFLFILPFGFRK-RKFIDCPEGKEDSYRSFANRW--LIFISMLL 68
           S S++I+ P+    LDLF  I+        KF    + +  +  ++ +RW   +FI  +L
Sbjct: 9   STSHLIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRWPVSVFICKIL 68

Query: 69  QLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYRSVVGF 128
           QLF    AA L  L   LL   NF   N G LG+L  +   +  + P     D+ S +G+
Sbjct: 69  QLF-QMPAAMLGHLTDFLL---NFYYQNHGFLGILRNIFLIRLKI-PKRGEADFISTIGY 128

Query: 129 TDWRKDL------------------DSSIKPDDTFRYYGA--------LTMMAAKVSYEF 188
            D R DL                  + S+K     +  GA        L +MA+K++YE 
Sbjct: 129 LDSRMDLHGTPMVSHQADEVISNADNPSLKEGHNSKIKGALGNRSLMDLCIMASKLAYEN 188

Query: 189 LPFIQSVVNDHWKMKMLHYYAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAY 248
              ++ VV +HWKM  +  Y   N FQ+   T AFIF +   D+N+ VI+FRGT P    
Sbjct: 189 TKVVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSIP 248

Query: 249 DWQVDADFSWYEIEGIGRIHSGFMKALGL------------------QKATGWPKNLPPS 308
           +W  D DFS   +   G +H GF++A+GL                     T   K    +
Sbjct: 249 NWCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEMA 308

Query: 309 PDNRQFA----YYTLRQQLRDIAKANDKARFIITGHSLGGALATLFVTLLSFHQESTLLE 368
           PD+  +A    Y+     L+ + K +  A+F++TGHSLGGALA LF  +L   QE+ +L+
Sbjct: 309 PDHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETEVLD 368

Query: 369 KLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIPADGIVFEYKHFGG 428
           +L  VYT+GQPR+G+     FM N ++    +Y+R VY ND+VPR+P D + F ++HFG 
Sbjct: 369 RLLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPFDDVFFTFEHFGT 428

Query: 429 CVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPIVKGSVYYEGLLMKG 464
           C++++S + G   +E+P++N F +   I  ++ AWWEL RSFI+  V G+ Y E    + 
Sbjct: 429 CIYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNHVYGAEYKETWESRM 488

BLAST of Spg016466 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.4e-10
Identity = 58/197 (29.44%), Postives = 90/197 (45.69%), Query Frame = 0

Query: 181 FWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGI---GR 240
           F   F++        F  +   + + V++FRG+  ++  +W  + +F   EI  I    R
Sbjct: 73  FLYSFEDSGVGDVTGFLALDNTNKLIVLSFRGSRSIE--NWIGNLNFDLKEINDICSGCR 132

Query: 241 IHSGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGG 300
            H GF         + W           +    TLRQ++ D  + +   R + TGHSLGG
Sbjct: 133 GHDGF--------TSSW-----------RSVADTLRQKVEDAVREHPDYRVVFTGHSLGG 192

Query: 301 ALATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSN 360
           ALAT+    L  +           V++YG PRVG++ FAEF+     + G   YR  ++N
Sbjct: 193 ALATVAGADLRGNGYDI------DVFSYGAPRVGNRAFAEFLT---VQTGGTLYRITHTN 237

Query: 361 DLVPRIPADGIVFEYKH 375
           D+VPR+P     F Y H
Sbjct: 253 DIVPRLPPR--EFGYSH 237

BLAST of Spg016466 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 1.2e-09
Identity = 58/179 (32.40%), Postives = 80/179 (44.69%), Query Frame = 0

Query: 202 DSNITV-IAFRGTSPLDAYDWQVDADF---SWYEIEGIGRIHSGFMKALGLQKATGWPKN 261
           DS  T+ I FRG+S +   +W  D  F   S+  + G  ++H GF+ + G          
Sbjct: 164 DSEKTIYIVFRGSSSI--RNWIADLTFVPVSYPPVSGT-KVHKGFLDSYG---------- 223

Query: 262 LPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFVTLLSFHQESTLLE 321
                 N   A       + D  K     +  +TGHSLGGA A L   L  + +E  L  
Sbjct: 224 ---EVQNELVA------TVLDQFKQYPSYKVAVTGHSLGGATA-LLCALDLYQREEGLSS 283

Query: 322 KLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIPADGIVFEYKHFG 377
               +YT GQPRVGD  FA ++V+T    G  Y R V   D+VP +P     F + H G
Sbjct: 284 SNLFLYTQGQPRVGDPAFANYVVST----GIPYRRTVNERDIVPHLPP--AAFGFLHAG 313

BLAST of Spg016466 vs. ExPASy TrEMBL
Match: A0A1S3BUY0 (uncharacterized protein LOC103493549 OS=Cucumis melo OX=3656 GN=LOC103493549 PE=4 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 2.6e-214
Identity = 351/483 (72.67%), Postives = 412/483 (85.30%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M+  E+EFSK+Y+ILKPENANLLDLFLF+LPFG RK+KFIDCP GKED + +FA+RWLIF
Sbjct: 1   MVLMENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFADRWLIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
           +SMLLQ  L  IA PLA LD+ LLK FNFISFNGG+LG+LSK+++ K FV+  E+S +Y 
Sbjct: 61  VSMLLQFILALIATPLAKLDAFLLKLFNFISFNGGLLGILSKILRGKGFVKVKEDSSEYT 120

Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
           SVVGF DWR+DLDSSIK + +FRYY ALT+MA K+SYE  PF+QSVVND WKMK+L Y+ 
Sbjct: 121 SVVGFADWRRDLDSSIKSEKSFRYYSALTVMATKISYESKPFVQSVVNDRWKMKLLGYFN 180

Query: 181 FWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHS 240
           FWNDFQ K TTQAF F+N A D N+ ++AFRGTSPLDAYDWQVD D SWYEIEG+GRIHS
Sbjct: 181 FWNDFQGKATTQAFTFENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHS 240

Query: 241 GFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALA 300
           GFMKALGLQKATGWPK LP +    +FAYYTLR+QLRDIAKAN+KARFI TGHSLGGALA
Sbjct: 241 GFMKALGLQKATGWPKELPKT-QTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALA 300

Query: 301 TLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLV 360
           TLFVT+LS H EST+LEKL +VYTYGQPRVGDQQFA+FMVN + KYGFKY+RYVYS DLV
Sbjct: 301 TLFVTVLSLHDESTILEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLV 360

Query: 361 PRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFI 420
           PR+P+D ++F+YKHFG CV+FNSLYKG+I++EQPN+NYFSL+W+IPKYL AWWELIRSFI
Sbjct: 361 PRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRSFI 420

Query: 421 IPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYV 480
           IP VKG  YYE LLMKG R+ GL+IPG +AHFP +YVN TRLG L +PD +EDP LG  +
Sbjct: 421 IPFVKGFDYYESLLMKGARLVGLLIPGLSAHFPLNYVNVTRLGKLNVPDEVEDPFLGYEI 480

Query: 481 EAD 484
           E D
Sbjct: 481 EDD 482

BLAST of Spg016466 vs. ExPASy TrEMBL
Match: A0A0A0L2D5 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639870 PE=4 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 1.9e-212
Identity = 350/483 (72.46%), Postives = 410/483 (84.89%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M+  E+EFSK+Y+ILKPENANLLDLFLF+LPFG RK+KFIDCP GKED + +F +RWLI 
Sbjct: 1   MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
           +SMLLQ  L  IA PLA LD+ LLK FNFISFNGG LG+LSK+++ K FV+ +E+S +Y 
Sbjct: 61  VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120

Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
           SVVGF DWR+DLDSSIKP+ +FRYY ALT MA K+SYE  PF+QSVVND WKMK+L Y+ 
Sbjct: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180

Query: 181 FWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHS 240
           FWNDFQ +TTTQAF+F+N A D N+T++AFRGTSPLDAYDWQVD DFSWYEIE +G IHS
Sbjct: 181 FWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHS 240

Query: 241 GFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALA 300
           GFMKALGLQKATGWPK LP +    +FAYYTLR+QLRDIAKAN+KARFI TGHSLGGALA
Sbjct: 241 GFMKALGLQKATGWPKELPKT-QTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALA 300

Query: 301 TLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLV 360
           TLFVT+L  H EST+LEKL +VYTYGQPRVGDQQFA+FM+N I KYGFKY+RYVYS DLV
Sbjct: 301 TLFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLV 360

Query: 361 PRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFI 420
           PR+P+D ++F+YKHFG CV+FNSLYKG+I++EQPNKNYFSL+W+IPKYL AWWELIRSFI
Sbjct: 361 PRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFI 420

Query: 421 IPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYV 480
           IP VKG  YYE LLMKG R+ GL+IPG  AHFP +YVN TRLG L +PD +EDPIL   +
Sbjct: 421 IPFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEI 480

Query: 481 EAD 484
           E D
Sbjct: 481 EDD 482

BLAST of Spg016466 vs. ExPASy TrEMBL
Match: A0A6J1FNK8 (uncharacterized protein LOC111445886 OS=Cucurbita moschata OX=3662 GN=LOC111445886 PE=4 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 2.3e-210
Identity = 353/484 (72.93%), Postives = 408/484 (84.30%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M+ KE EFSK+YMILKP +A LLDLFLF+LPFG RK KFIDCPEG EDSY +F +RW IF
Sbjct: 1   MVWKECEFSKNYMILKPNDAKLLDLFLFLLPFGLRKTKFIDCPEGNEDSYNNFTDRWSIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
           IS+LLQ+ L A A PLA LDS L+  FNFISFNGGILGLL+K++K K  V+P+E+S DY 
Sbjct: 61  ISVLLQIILAAAATPLAKLDSFLVNLFNFISFNGGILGLLAKILKGKGLVKPNEDSSDYT 120

Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
           SVVGF DWR DLDSSI   DTFRYYG LT+MAAK++YE  PF+QSVVND WKMK+L ++ 
Sbjct: 121 SVVGFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFN 180

Query: 181 FWNDFQNKTTTQAFIFQNIATDS-NITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIH 240
           FWNDFQ K TTQAF+FQN ATD  NI VIAFRGTSPLD YDWQVDADFSWY+IEG+GRIH
Sbjct: 181 FWNDFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTYDWQVDADFSWYDIEGVGRIH 240

Query: 241 SGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGAL 300
           SGFMKALGLQKATGWPK L  +     FAYYTLRQ+LRDIAKAN KARFIITGHSLGGAL
Sbjct: 241 SGFMKALGLQKATGWPKEL--TKPQHDFAYYTLRQKLRDIAKANAKARFIITGHSLGGAL 300

Query: 301 ATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDL 360
           ATLFVTLLS H E+ +LEKLQ VYTYGQPRVGD++FAEFMVN++ +YGFKYYRYVYS+DL
Sbjct: 301 ATLFVTLLSLHDETPILEKLQGVYTYGQPRVGDRRFAEFMVNSVQQYGFKYYRYVYSSDL 360

Query: 361 VPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSF 420
           VPRIP+DG++F+YKHFG  ++FN+LYKG+I++ QPNKNYFSL+W+IPKYL AWWEL+RSF
Sbjct: 361 VPRIPSDGVIFKYKHFGRSIYFNTLYKGRIVKGQPNKNYFSLLWVIPKYLSAWWELVRSF 420

Query: 421 IIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNY 480
           I P+V G  YYE LLM   R+ GLIIPG AAHFP +YVNSTRLG L+ PD +EDPIL + 
Sbjct: 421 ITPLVWGFDYYESLLMSAARIIGLIIPGLAAHFPLNYVNSTRLGKLKAPDEVEDPILEDD 480

Query: 481 VEAD 484
           +E D
Sbjct: 481 IEGD 482

BLAST of Spg016466 vs. ExPASy TrEMBL
Match: A0A6J1CEI9 (uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010491 PE=4 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 1.6e-208
Identity = 346/470 (73.62%), Postives = 406/470 (86.38%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKR-KFIDCPEGKEDSY-RSFANRWL 60
           M SKEHEFSK+Y+ILKP+NAN++DLFLF+LPFGFRKR KFIDCPEGKEDSY  SF NRWL
Sbjct: 1   MASKEHEFSKNYLILKPQNANIVDLFLFLLPFGFRKRTKFIDCPEGKEDSYTSSFTNRWL 60

Query: 61  IFISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPD 120
           IFIS++LQ+ L AIA PLA LD+ LL  FNFISFNGGILGLLS+++K +  V+PDENS +
Sbjct: 61  IFISIVLQIILLAIATPLAKLDAFLLNVFNFISFNGGILGLLSRILKGESLVKPDENSAE 120

Query: 121 YRSVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHY 180
           Y SVVGF DWR+DLD SIK ++ FRYYG LT+MAA++SYE  PF+ SVVND WKMK+L +
Sbjct: 121 YTSVVGFADWRRDLDKSIKAEEIFRYYGGLTVMAAQLSYESKPFVLSVVNDRWKMKLLGH 180

Query: 181 YAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRI 240
           + FWNDFQ K TTQAF+FQN A D N+TV+AFRGTSPLDAYDWQVDADFSWY+IEG+GRI
Sbjct: 181 FNFWNDFQGKATTQAFMFQNTAADPNVTVVAFRGTSPLDAYDWQVDADFSWYDIEGVGRI 240

Query: 241 HSGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGA 300
           HSGFMKALGLQKATGWP+ L  S     FAYYTLRQ+LRDIAK+ND A+FIITGHSLGGA
Sbjct: 241 HSGFMKALGLQKATGWPEELVKS--KHHFAYYTLRQKLRDIAKSNDNAKFIITGHSLGGA 300

Query: 301 LATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSND 360
           LA LFVT+LS H++S +LEKLQAVYTY QPRVGD++FAEFMVNT+ KYGFKY+RYVYS+D
Sbjct: 301 LAALFVTVLSLHRDSAVLEKLQAVYTYSQPRVGDRRFAEFMVNTVKKYGFKYHRYVYSSD 360

Query: 361 LVPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRS 420
           LVPR+P DG++F+YKHFG C++FNSLYKG+I++E+PNKNYFSL+W++PKYL AWWELIRS
Sbjct: 361 LVPRVPFDGVIFKYKHFGRCIYFNSLYKGRIVKEEPNKNYFSLIWVMPKYLNAWWELIRS 420

Query: 421 FIIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIP 469
           FI+P+VKG  Y+E LLMKG RV GL IPG AAH P DYVNS RLG    P
Sbjct: 421 FIVPLVKGFDYHESLLMKGARVVGLFIPGLAAHIPTDYVNSIRLGKFNPP 468

BLAST of Spg016466 vs. ExPASy TrEMBL
Match: A0A6J1J3Y9 (uncharacterized protein LOC111481121 OS=Cucurbita maxima OX=3661 GN=LOC111481121 PE=4 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 3.6e-208
Identity = 350/484 (72.31%), Postives = 409/484 (84.50%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M+SK+ EFSK+YMILKP +A LLDLFLF+LPFG RK KFIDCPEG E SY +F +RW IF
Sbjct: 1   MVSKKCEFSKNYMILKPNDAKLLDLFLFLLPFGLRKTKFIDCPEGNEASYSNFTDRWFIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
           IS+LLQ+ L A A PL+ LDS L+  FNFISFNGGILGLLSK++K K  V+P+E+S DY 
Sbjct: 61  ISILLQIILAAAATPLSKLDSFLVNLFNFISFNGGILGLLSKILKGKGLVKPNEDSSDYT 120

Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
           SV+GF DWR DLDSSI   DTFRYYG LT+MAAK++YE  PF+QSVVND WKMK+L ++ 
Sbjct: 121 SVLGFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFN 180

Query: 181 FWNDFQNKTTTQAFIFQNIATDS-NITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIH 240
           FWNDFQ K TTQAF+FQN ATD  NI VIAFRGTSPLD  DWQV+ADFSWY+IEG+GRIH
Sbjct: 181 FWNDFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTDDWQVNADFSWYDIEGLGRIH 240

Query: 241 SGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGAL 300
           SGFMKALGLQKATGWPK L  +     FAYY+LRQ+LRDIAKAN KARFIITGHSLGGAL
Sbjct: 241 SGFMKALGLQKATGWPKEL--TKPQHDFAYYSLRQKLRDIAKANAKARFIITGHSLGGAL 300

Query: 301 ATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDL 360
           ATLFVTLLS H E+T+LEKLQ VYTYGQPRVGD++FAEFMVNT+ KYGFKYYRYVYS+DL
Sbjct: 301 ATLFVTLLSLHDETTILEKLQGVYTYGQPRVGDRRFAEFMVNTVQKYGFKYYRYVYSSDL 360

Query: 361 VPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSF 420
           VPRIP+DG++F+YKHFG  ++FNSLYKG+I++EQPNKNYFSL+W+IPKYL AWWEL+RSF
Sbjct: 361 VPRIPSDGVIFKYKHFGRSIYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELVRSF 420

Query: 421 IIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNY 480
           I P++ G  +YE LLM   R+ GLIIPG AAHFP +YVNS RLG L++PD IEDPIL + 
Sbjct: 421 ITPLMWGFDHYESLLMSAARMVGLIIPGLAAHFPLNYVNSIRLGKLKVPDEIEDPILEDD 480

Query: 481 VEAD 484
           +E +
Sbjct: 481 IEGE 482

BLAST of Spg016466 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 431.4 bits (1108), Expect = 9.7e-121
Identity = 217/471 (46.07%), Postives = 301/471 (63.91%), Query Frame = 0

Query: 7   EFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSY-RSFANRWLIFISMLL 66
           +F  SY ++ P  A+ LDL L +        +FID P      + RSFA+RW++ +++ L
Sbjct: 7   KFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFL 66

Query: 67  QLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYRSVVGF 126
           Q  L  ++ P A +   L    N ++ NGG   L+  L+  K  V+PD++S  Y S +G 
Sbjct: 67  QKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGK-LVKPDKSSATYTSFIGC 126

Query: 127 TDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWNDF 186
           +D R +LD  I    +  Y   L++MA+K+SYE  P+I SVV + WKM ++  Y F+N F
Sbjct: 127 SDRRIELDEKINV-GSIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAF 186

Query: 187 QNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKA 246
           Q    TQAF+F+  +T+ ++ V++FRGT P +A DW  D D SWYE++ +G++H+GF +A
Sbjct: 187 QESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRA 246

Query: 247 LGLQKATGWPK-NLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFV 306
           LGLQK  GWPK N+       Q+AYYT+RQ LRD    N   ++I+TGHSLGGALA LF 
Sbjct: 247 LGLQK-DGWPKENISLL---HQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFP 306

Query: 307 TLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIP 366
            +L+ H E  LL+KL+ +YT+GQPRVGD+ F EFM   + K+G +Y R+VY+ND+VPR+P
Sbjct: 307 AILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVP 366

Query: 367 ADG-IVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPI 426
            D   +F YKH+G C  FNSLYKGK+  + PN NYF+L+WLIP+ L   WE IRSFI+  
Sbjct: 367 FDDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTGLWEFIRSFILQF 426

Query: 427 VKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDP 475
            KG  Y E  LM+ VRV G++ PG + HFP DYVNSTRLG L  P     P
Sbjct: 427 WKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRPPPTTTP 471

BLAST of Spg016466 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 422.9 bits (1086), Expect = 3.5e-118
Identity = 206/465 (44.30%), Postives = 301/465 (64.73%), Query Frame = 0

Query: 9   SKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGK-EDSYRSFANRWLIFISMLLQL 68
           +K+Y +L P  A + DL   +       RKFI   E + ED    F  RW+IF+S+++Q 
Sbjct: 4   TKNYFVLDPREATVSDLIHLLFSSDLGDRKFIHSSEERIEDDLSRFRRRWIIFVSIVIQK 63

Query: 69  FLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYRSVVGFTD 128
            +     PL  +   L    N +S NGG L +L  L K    + P++ S  + S+ G  D
Sbjct: 64  LIILFKKPLYFVGFSLACWLNLLSSNGGFLMILPNLFKGN-IIWPEKASATFASLNGNLD 123

Query: 129 WRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWNDFQN 188
            R +L+  ++     RY   L++MA+K+SYE + F+ SV+++HWKM +L +Y+ WN +Q 
Sbjct: 124 RRVELNPKVERGSK-RYKAMLSIMASKLSYENINFVSSVLHNHWKMDLLGFYSCWNGYQK 183

Query: 189 KTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKALG 248
           + +T+  + ++ +TD N+ +++FRGT P DA DW  D D SWYE++ +G+IH GFMKALG
Sbjct: 184 QRSTEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKIHGGFMKALG 243

Query: 249 LQKATGWPK--NLPPSPD-NRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFV 308
           LQK  GWPK  NL  + +    +AYYT+R+ L++I   N  ++FI+TGHSLGGALA LF 
Sbjct: 244 LQK-EGWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTGHSLGGALAILFT 303

Query: 309 TLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIP 368
            +L  H E  +LE+L+ VYT+GQPRVGD++F  FM +++ K+  KY RYVY ND+VPR+P
Sbjct: 304 AVLVMHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDSLKKFDVKYERYVYCNDMVPRLP 363

Query: 369 ADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPIV 428
            D     +KHFG C++++S YKGK+  E+PNKNYF+LVW++PK + A WELIRSF++P  
Sbjct: 364 FDDKTLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKIMNALWELIRSFVMPYW 423

Query: 429 KGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPD 470
           KG  + EG  ++  RV  L+IPG  AHFP +Y+N T LG   +PD
Sbjct: 424 KGGEFREGWFLRCFRVVALLIPGLPAHFPNEYINVTLLG--DLPD 463

BLAST of Spg016466 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 393.7 bits (1010), Expect = 2.2e-109
Identity = 206/480 (42.92%), Postives = 284/480 (59.17%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M S + +  + Y+IL+PE     +L   +      K + +D  E +E S+R   +RWLIF
Sbjct: 2   MNSDDDDEPRGYLILRPEELRPWELVRLLFSGDIEKPRSVDSSETEEHSFR---HRWLIF 61

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
           +S++L   L   +  LA + S L    NF+S N       S L  R   V P   S +Y+
Sbjct: 62  VSLVLLKLLRFFSKLLALVGSALEFSLNFLSNNS-----FSGLFLRGEVVMPQRTSENYQ 121

Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
           S +G  D R  LD ++  +D  +YY AL++MA+K++YE    I+ VV +HW MK L    
Sbjct: 122 SFIGHLDTRVSLDMTLNREDGEKYYAALSIMASKIAYENAARIKHVVENHWNMKYLGLVD 181

Query: 181 FWNDFQNKTTTQAFIFQNIAT------DSNITVIAFRGTSPLDAYDWQVDADFSWYEIEG 240
           +WN++Q K TTQAFI     T           V+AFRGT   ++ DW  D D +W+E+  
Sbjct: 182 YWNEYQEKETTQAFIMSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDFDITWFELPN 241

Query: 241 IGRIHSGFMKALGLQKATGWPKNLPPSPDNRQ-FAYYTLRQQLRDIAKANDKARFIITGH 300
           IG IH GFMKALGLQ    WPK    +PD +   AYY++R  L+ +   N   +F++TGH
Sbjct: 242 IGNIHGGFMKALGLQNNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTKFVLTGH 301

Query: 301 SLGGALATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRY 360
           SLGGALA LF  +L  H E+ LLE++Q VYTYGQPRVGD +F EFM   + KY  KYYR+
Sbjct: 302 SLGGALAILFTAVLVIHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYNIKYYRF 361

Query: 361 VYSNDLVPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWW 420
           VY+ND+VPR+P D     +KHFG C++++  Y+ K++REQ ++N+F L  +I     A  
Sbjct: 362 VYNNDIVPRLPYDDKDLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIKMMWSAIL 421

Query: 421 ELIRSFIIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIED 474
           E IRSF I   KGS Y EG L+KG R  G+I+PG + H PQDYVN+TRL L  +  V  D
Sbjct: 422 EFIRSFTIVAEKGSEYSEGWLLKGGRALGIIVPGVSNHTPQDYVNATRLTLPCVFQVYRD 473

BLAST of Spg016466 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 387.9 bits (995), Expect = 1.2e-107
Identity = 200/468 (42.74%), Postives = 288/468 (61.54%), Query Frame = 0

Query: 10  KSYMILKPENANLLDLFLFILPFGFRKRKFIDCP-EGKEDSYRSFANRWLIFISMLLQLF 69
           K+Y +L P  A + DL   +       RKF+D   E  E+    F  RW+IF+S+++Q  
Sbjct: 5   KNYFVLDPREATVTDLMRLLFYSDLENRKFVDTSVENLENRLCEFRGRWIIFVSIVVQKL 64

Query: 70  LGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYRSVVGFTDW 129
           L  +  PL+ L   L    N  S NGG   +   LVK + F+ P++ S  + S+ G  D 
Sbjct: 65  LIILRKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGR-FIWPEKTSATFASINGNLDQ 124

Query: 130 RKD--LDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWNDFQ 189
           + +  L  SIK  D  RY   L++MA+K++YE   FI+SV+ DHW+M +L +Y+  NDF 
Sbjct: 125 KVELGLGRSIKIGDE-RYKPLLSIMASKLAYENEHFIRSVLQDHWQMDLLGFYSCPNDFD 184

Query: 190 NKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKAL 249
              +T+  + ++   + N+ V++FRGT P +A DW  D D SW+ +  +G+IH GFMKAL
Sbjct: 185 QTRSTEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGKIHGGFMKAL 244

Query: 250 GLQKATGWPKNLPPSPDNR---QFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLF 309
           GL K  GW + +          Q AYYT+ +QL+++ + N  ++FI++GHSLGGALA LF
Sbjct: 245 GLPK-EGWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSLGGALAILF 304

Query: 310 VTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRI 369
             +L  H E  +LE+L+ VYT+GQPRVGD+ F  +M + + ++  KY RYVY ND+VPR+
Sbjct: 305 TAVLIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVYCNDMVPRL 364

Query: 370 PADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPI 429
           P D     +KHFGGC++ +S YKGK+  E+PNKNYF++ W+IPK + A WELIRSFII  
Sbjct: 365 PFDDKTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKIINAVWELIRSFIISC 424

Query: 430 VKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLL--QIPD 470
            +G  Y EG L+   R+  L+IPG  AHFP +YVN   LG    Q+PD
Sbjct: 425 WQGREYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLGNFPPQVPD 469

BLAST of Spg016466 vs. TAIR 10
Match: AT1G45201.2 (triacylglycerol lipase-like 1 )

HSP 1 Score: 342.0 bits (876), Expect = 7.8e-94
Identity = 172/386 (44.56%), Postives = 247/386 (63.99%), Query Frame = 0

Query: 7   EFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSY-RSFANRWLIFISMLL 66
           +F  SY ++ P  A+ LDL L +        +FID P      + RSFA+RW++ +++ L
Sbjct: 7   KFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFL 66

Query: 67  QLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYRSVVGF 126
           Q  L  ++ P A +   L    N ++ NGG   L+  L+  K  V+PD++S  Y S +G 
Sbjct: 67  QKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGK-LVKPDKSSATYTSFIGC 126

Query: 127 TDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWNDF 186
           +D R +LD  I    +  Y   L++MA+K+SYE  P+I SVV + WKM ++  Y F+N F
Sbjct: 127 SDRRIELDEKINV-GSIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAF 186

Query: 187 QNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKA 246
           Q    TQAF+F+  +T+ ++ V++FRGT P +A DW  D D SWYE++ +G++H+GF +A
Sbjct: 187 QESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRA 246

Query: 247 LGLQKATGWPK-NLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFV 306
           LGLQK  GWPK N+       Q+AYYT+RQ LRD    N   ++I+TGHSLGGALA LF 
Sbjct: 247 LGLQK-DGWPKENISLL---HQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFP 306

Query: 307 TLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIP 366
            +L+ H E  LL+KL+ +YT+GQPRVGD+ F EFM   + K+G +Y R+VY+ND+VPR+P
Sbjct: 307 AILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVP 366

Query: 367 ADG-IVFEYKHFGGCVFFNSLYKGKI 390
            D   +F YKH+G C  FNSLYKGK+
Sbjct: 367 FDDKYLFSYKHYGPCNSFNSLYKGKV 386

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038899591.16.6e-22075.99triacylglycerol lipase OBL1-like [Benincasa hispida][more]
XP_008452563.15.4e-21472.67PREDICTED: uncharacterized protein LOC103493549 [Cucumis melo][more]
XP_011654113.13.9e-21272.46uncharacterized protein LOC101203640 [Cucumis sativus] >KGN55199.1 hypothetical ... [more]
XP_022940183.14.7e-21072.93uncharacterized protein LOC111445886 [Cucurbita moschata][more]
XP_022139642.13.4e-20873.62uncharacterized protein LOC111010491 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A1S3ZP856.4e-8537.16Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1[more]
F4JFU81.6e-8334.76Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1[more]
Q5VKJ75.4e-7634.78Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1[more]
O599521.4e-1029.44Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1[more]
P195151.2e-0932.40Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S3BUY02.6e-21472.67uncharacterized protein LOC103493549 OS=Cucumis melo OX=3656 GN=LOC103493549 PE=... [more]
A0A0A0L2D51.9e-21272.46Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639870 PE... [more]
A0A6J1FNK82.3e-21072.93uncharacterized protein LOC111445886 OS=Cucurbita moschata OX=3662 GN=LOC1114458... [more]
A0A6J1CEI91.6e-20873.62uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A6J1J3Y93.6e-20872.31uncharacterized protein LOC111481121 OS=Cucurbita maxima OX=3661 GN=LOC111481121... [more]
Match NameE-valueIdentityDescription
AT1G45201.19.7e-12146.07triacylglycerol lipase-like 1 [more]
AT5G42930.13.5e-11844.30alpha/beta-Hydrolases superfamily protein [more]
AT5G67050.12.2e-10942.92alpha/beta-Hydrolases superfamily protein [more]
AT1G56630.11.2e-10742.74alpha/beta-Hydrolases superfamily protein [more]
AT1G45201.27.8e-9444.56triacylglycerol lipase-like 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 207..366
e-value: 9.2E-37
score: 126.1
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 143..401
e-value: 1.3E-47
score: 164.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 178..399
IPR044819Triacylglycerol lipase OBL1-likePANTHERPTHR46086ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 4..466
NoneNo IPR availablePANTHERPTHR46086:SF4ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 4..466
NoneNo IPR availableCDDcd00519Lipase_3coord: 140..382
e-value: 2.27334E-42
score: 148.779

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg016466.1Spg016466.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004806 triglyceride lipase activity