Homology
BLAST of Spg016466 vs. NCBI nr
Match:
XP_038899591.1 (triacylglycerol lipase OBL1-like [Benincasa hispida])
HSP 1 Score: 774.2 bits (1998), Expect = 6.6e-220
Identity = 364/479 (75.99%), Postives = 419/479 (87.47%), Query Frame = 0
Query: 5 EHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIFISML 64
E EFSK+Y+ILKPENANLLDLFLF+LPFG RKRKFIDCP GKED +++F +RWLIFISML
Sbjct: 5 ESEFSKNYVILKPENANLLDLFLFLLPFGLRKRKFIDCPLGKEDPFKNFRDRWLIFISML 64
Query: 65 LQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYRSVVG 124
LQ L IA PLA LDS LLK NFISFNGG+ G+LSK++K K F++ +E+SP+Y SVVG
Sbjct: 65 LQFILAIIATPLAKLDSFLLKLLNFISFNGGVSGILSKILKGKSFLEVNEDSPEYTSVVG 124
Query: 125 FTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWND 184
F DWR+DLDSSIKPDDTFRYY ALT+MAAKVSYE PF+QSVVND WKMK+L Y+ FWND
Sbjct: 125 FADWRRDLDSSIKPDDTFRYYSALTVMAAKVSYESKPFVQSVVNDRWKMKLLGYFNFWND 184
Query: 185 FQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMK 244
FQ K TTQAF+F+N A D N+ V+AFRGTS LD+YDWQVDADFSWYEIEG+GRIHSGFMK
Sbjct: 185 FQGKATTQAFMFENTAIDPNVIVVAFRGTSLLDSYDWQVDADFSWYEIEGVGRIHSGFMK 244
Query: 245 ALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFV 304
ALGLQKATGWPK LP S ++FAYYT+RQ+LRDIAK NDKARFIITGHSLGGALATLFV
Sbjct: 245 ALGLQKATGWPKELPKS-QLQEFAYYTIRQKLRDIAKTNDKARFIITGHSLGGALATLFV 304
Query: 305 TLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIP 364
T+LS H EST+LEKL+ +YTYGQPRVGDQQFA+FMVNT+ KYGFKY+RYVYS+DLVPR+P
Sbjct: 305 TVLSLHDESTILEKLEGIYTYGQPRVGDQQFAKFMVNTVQKYGFKYHRYVYSSDLVPRLP 364
Query: 365 ADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPIV 424
+D I+F+YKHFG CVFFN+LY+G+I++EQPNKNYFSLVW+IPKYL AWWELIRS IIP+V
Sbjct: 365 SDAILFKYKHFGRCVFFNTLYRGRIVKEQPNKNYFSLVWVIPKYLSAWWELIRSLIIPLV 424
Query: 425 KGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYVEAD 484
KG YYE LLMKG RV GL IPG AAHFP +YVNSTRLG L +PD +EDPILG+ +E D
Sbjct: 425 KGFDYYESLLMKGGRVVGLFIPGLAAHFPLNYVNSTRLGKLNVPDKVEDPILGDDIEDD 482
BLAST of Spg016466 vs. NCBI nr
Match:
XP_008452563.1 (PREDICTED: uncharacterized protein LOC103493549 [Cucumis melo])
HSP 1 Score: 754.6 bits (1947), Expect = 5.4e-214
Identity = 351/483 (72.67%), Postives = 412/483 (85.30%), Query Frame = 0
Query: 1 MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
M+ E+EFSK+Y+ILKPENANLLDLFLF+LPFG RK+KFIDCP GKED + +FA+RWLIF
Sbjct: 1 MVLMENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFADRWLIF 60
Query: 61 ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
+SMLLQ L IA PLA LD+ LLK FNFISFNGG+LG+LSK+++ K FV+ E+S +Y
Sbjct: 61 VSMLLQFILALIATPLAKLDAFLLKLFNFISFNGGLLGILSKILRGKGFVKVKEDSSEYT 120
Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
SVVGF DWR+DLDSSIK + +FRYY ALT+MA K+SYE PF+QSVVND WKMK+L Y+
Sbjct: 121 SVVGFADWRRDLDSSIKSEKSFRYYSALTVMATKISYESKPFVQSVVNDRWKMKLLGYFN 180
Query: 181 FWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHS 240
FWNDFQ K TTQAF F+N A D N+ ++AFRGTSPLDAYDWQVD D SWYEIEG+GRIHS
Sbjct: 181 FWNDFQGKATTQAFTFENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHS 240
Query: 241 GFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALA 300
GFMKALGLQKATGWPK LP + +FAYYTLR+QLRDIAKAN+KARFI TGHSLGGALA
Sbjct: 241 GFMKALGLQKATGWPKELPKT-QTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALA 300
Query: 301 TLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLV 360
TLFVT+LS H EST+LEKL +VYTYGQPRVGDQQFA+FMVN + KYGFKY+RYVYS DLV
Sbjct: 301 TLFVTVLSLHDESTILEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLV 360
Query: 361 PRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFI 420
PR+P+D ++F+YKHFG CV+FNSLYKG+I++EQPN+NYFSL+W+IPKYL AWWELIRSFI
Sbjct: 361 PRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRSFI 420
Query: 421 IPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYV 480
IP VKG YYE LLMKG R+ GL+IPG +AHFP +YVN TRLG L +PD +EDP LG +
Sbjct: 421 IPFVKGFDYYESLLMKGARLVGLLIPGLSAHFPLNYVNVTRLGKLNVPDEVEDPFLGYEI 480
Query: 481 EAD 484
E D
Sbjct: 481 EDD 482
BLAST of Spg016466 vs. NCBI nr
Match:
XP_011654113.1 (uncharacterized protein LOC101203640 [Cucumis sativus] >KGN55199.1 hypothetical protein Csa_012910 [Cucumis sativus])
HSP 1 Score: 748.4 bits (1931), Expect = 3.9e-212
Identity = 350/483 (72.46%), Postives = 410/483 (84.89%), Query Frame = 0
Query: 1 MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
M+ E+EFSK+Y+ILKPENANLLDLFLF+LPFG RK+KFIDCP GKED + +F +RWLI
Sbjct: 1 MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60
Query: 61 ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
+SMLLQ L IA PLA LD+ LLK FNFISFNGG LG+LSK+++ K FV+ +E+S +Y
Sbjct: 61 VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120
Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
SVVGF DWR+DLDSSIKP+ +FRYY ALT MA K+SYE PF+QSVVND WKMK+L Y+
Sbjct: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180
Query: 181 FWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHS 240
FWNDFQ +TTTQAF+F+N A D N+T++AFRGTSPLDAYDWQVD DFSWYEIE +G IHS
Sbjct: 181 FWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHS 240
Query: 241 GFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALA 300
GFMKALGLQKATGWPK LP + +FAYYTLR+QLRDIAKAN+KARFI TGHSLGGALA
Sbjct: 241 GFMKALGLQKATGWPKELPKT-QTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALA 300
Query: 301 TLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLV 360
TLFVT+L H EST+LEKL +VYTYGQPRVGDQQFA+FM+N I KYGFKY+RYVYS DLV
Sbjct: 301 TLFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLV 360
Query: 361 PRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFI 420
PR+P+D ++F+YKHFG CV+FNSLYKG+I++EQPNKNYFSL+W+IPKYL AWWELIRSFI
Sbjct: 361 PRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFI 420
Query: 421 IPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYV 480
IP VKG YYE LLMKG R+ GL+IPG AHFP +YVN TRLG L +PD +EDPIL +
Sbjct: 421 IPFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEI 480
Query: 481 EAD 484
E D
Sbjct: 481 EDD 482
BLAST of Spg016466 vs. NCBI nr
Match:
XP_022940183.1 (uncharacterized protein LOC111445886 [Cucurbita moschata])
HSP 1 Score: 741.5 bits (1913), Expect = 4.7e-210
Identity = 353/484 (72.93%), Postives = 408/484 (84.30%), Query Frame = 0
Query: 1 MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
M+ KE EFSK+YMILKP +A LLDLFLF+LPFG RK KFIDCPEG EDSY +F +RW IF
Sbjct: 1 MVWKECEFSKNYMILKPNDAKLLDLFLFLLPFGLRKTKFIDCPEGNEDSYNNFTDRWSIF 60
Query: 61 ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
IS+LLQ+ L A A PLA LDS L+ FNFISFNGGILGLL+K++K K V+P+E+S DY
Sbjct: 61 ISVLLQIILAAAATPLAKLDSFLVNLFNFISFNGGILGLLAKILKGKGLVKPNEDSSDYT 120
Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
SVVGF DWR DLDSSI DTFRYYG LT+MAAK++YE PF+QSVVND WKMK+L ++
Sbjct: 121 SVVGFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFN 180
Query: 181 FWNDFQNKTTTQAFIFQNIATDS-NITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIH 240
FWNDFQ K TTQAF+FQN ATD NI VIAFRGTSPLD YDWQVDADFSWY+IEG+GRIH
Sbjct: 181 FWNDFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTYDWQVDADFSWYDIEGVGRIH 240
Query: 241 SGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGAL 300
SGFMKALGLQKATGWPK L + FAYYTLRQ+LRDIAKAN KARFIITGHSLGGAL
Sbjct: 241 SGFMKALGLQKATGWPKEL--TKPQHDFAYYTLRQKLRDIAKANAKARFIITGHSLGGAL 300
Query: 301 ATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDL 360
ATLFVTLLS H E+ +LEKLQ VYTYGQPRVGD++FAEFMVN++ +YGFKYYRYVYS+DL
Sbjct: 301 ATLFVTLLSLHDETPILEKLQGVYTYGQPRVGDRRFAEFMVNSVQQYGFKYYRYVYSSDL 360
Query: 361 VPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSF 420
VPRIP+DG++F+YKHFG ++FN+LYKG+I++ QPNKNYFSL+W+IPKYL AWWEL+RSF
Sbjct: 361 VPRIPSDGVIFKYKHFGRSIYFNTLYKGRIVKGQPNKNYFSLLWVIPKYLSAWWELVRSF 420
Query: 421 IIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNY 480
I P+V G YYE LLM R+ GLIIPG AAHFP +YVNSTRLG L+ PD +EDPIL +
Sbjct: 421 ITPLVWGFDYYESLLMSAARIIGLIIPGLAAHFPLNYVNSTRLGKLKAPDEVEDPILEDD 480
Query: 481 VEAD 484
+E D
Sbjct: 481 IEGD 482
BLAST of Spg016466 vs. NCBI nr
Match:
XP_022139642.1 (uncharacterized protein LOC111010491 [Momordica charantia])
HSP 1 Score: 735.3 bits (1897), Expect = 3.4e-208
Identity = 346/470 (73.62%), Postives = 406/470 (86.38%), Query Frame = 0
Query: 1 MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKR-KFIDCPEGKEDSY-RSFANRWL 60
M SKEHEFSK+Y+ILKP+NAN++DLFLF+LPFGFRKR KFIDCPEGKEDSY SF NRWL
Sbjct: 1 MASKEHEFSKNYLILKPQNANIVDLFLFLLPFGFRKRTKFIDCPEGKEDSYTSSFTNRWL 60
Query: 61 IFISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPD 120
IFIS++LQ+ L AIA PLA LD+ LL FNFISFNGGILGLLS+++K + V+PDENS +
Sbjct: 61 IFISIVLQIILLAIATPLAKLDAFLLNVFNFISFNGGILGLLSRILKGESLVKPDENSAE 120
Query: 121 YRSVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHY 180
Y SVVGF DWR+DLD SIK ++ FRYYG LT+MAA++SYE PF+ SVVND WKMK+L +
Sbjct: 121 YTSVVGFADWRRDLDKSIKAEEIFRYYGGLTVMAAQLSYESKPFVLSVVNDRWKMKLLGH 180
Query: 181 YAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRI 240
+ FWNDFQ K TTQAF+FQN A D N+TV+AFRGTSPLDAYDWQVDADFSWY+IEG+GRI
Sbjct: 181 FNFWNDFQGKATTQAFMFQNTAADPNVTVVAFRGTSPLDAYDWQVDADFSWYDIEGVGRI 240
Query: 241 HSGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGA 300
HSGFMKALGLQKATGWP+ L S FAYYTLRQ+LRDIAK+ND A+FIITGHSLGGA
Sbjct: 241 HSGFMKALGLQKATGWPEELVKS--KHHFAYYTLRQKLRDIAKSNDNAKFIITGHSLGGA 300
Query: 301 LATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSND 360
LA LFVT+LS H++S +LEKLQAVYTY QPRVGD++FAEFMVNT+ KYGFKY+RYVYS+D
Sbjct: 301 LAALFVTVLSLHRDSAVLEKLQAVYTYSQPRVGDRRFAEFMVNTVKKYGFKYHRYVYSSD 360
Query: 361 LVPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRS 420
LVPR+P DG++F+YKHFG C++FNSLYKG+I++E+PNKNYFSL+W++PKYL AWWELIRS
Sbjct: 361 LVPRVPFDGVIFKYKHFGRCIYFNSLYKGRIVKEEPNKNYFSLIWVMPKYLNAWWELIRS 420
Query: 421 FIIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIP 469
FI+P+VKG Y+E LLMKG RV GL IPG AAH P DYVNS RLG P
Sbjct: 421 FIVPLVKGFDYHESLLMKGARVVGLFIPGLAAHIPTDYVNSIRLGKFNPP 468
BLAST of Spg016466 vs. ExPASy Swiss-Prot
Match:
A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)
HSP 1 Score: 316.2 bits (809), Expect = 6.4e-85
Identity = 178/479 (37.16%), Postives = 257/479 (53.65%), Query Frame = 0
Query: 55 NRWLIFISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDE 114
+RW+IF+S++++ + P+ ++ N S NG LGLL ++ K V P
Sbjct: 85 HRWVIFVSIIVRKLIAIFGKPMEWTGYLVEFFLNLFSLNGNFLGLLYNILHGK-VVMPHR 144
Query: 115 NSPDYRSVVGFTDWR------KDLDSSIKPDDTFRYYGA-------LTMMAAKVSYEFLP 174
S + S +G D R + L I D ++ G L MMA+K++YE
Sbjct: 145 GSETFISAIGHLDGRINLYKSETLTKEIGEPDFWQKIGIGHRDLMDLCMMASKLAYENEK 204
Query: 175 FIQSVVNDHWKMKMLHYYAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDW 234
+++VVN HWKM + +Y WNDF+ + +TQ F+ + D+N+ +++FRGT P DA DW
Sbjct: 205 VVRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDANLILVSFRGTEPFDADDW 264
Query: 235 QVDADFSWYEIEGIGRIHSGFMKALGLQKATG--------WPKNL------------PPS 294
D D+SWYEI +G++H GF++ALGL T + NL PPS
Sbjct: 265 ITDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFVNNLKFANLENVHATIPPS 324
Query: 295 PDNR-------------------------------------QFAYYTLRQQLRDIAKAND 354
++ + AYY +R +L+ + K +
Sbjct: 325 ESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERTAYYVVRSKLKRLLKEHK 384
Query: 355 KARFIITGHSLGGALATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIH 414
A+F++TGHSLGGALA LF +L H+E ++E+L +YTYGQPRVG++Q FM +
Sbjct: 385 NAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRVGNRQLGRFMEAHLE 444
Query: 415 KYGFKYYRYVYSNDLVPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWL 464
KY+R VY NDLVPR+P D F +KHFG C ++NSLY + + E+PN NYF + +L
Sbjct: 445 HPVPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNSLYIEQNINEEPNPNYFGMRFL 504
BLAST of Spg016466 vs. ExPASy Swiss-Prot
Match:
F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)
HSP 1 Score: 311.6 bits (797), Expect = 1.6e-83
Identity = 171/492 (34.76%), Postives = 273/492 (55.49%), Query Frame = 0
Query: 11 SYMILKPENANLLDLFLF-ILPFGFRKRKFIDCPEGKEDS-----YRSFANRWLIFISML 70
+Y+I++P +DLF + + K KF++ P+ +E S + +RW+I +S+L
Sbjct: 18 NYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDHRWVIVVSIL 77
Query: 71 LQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYRSVVG 130
++ + + P+ ++ N S NGG GLL +L++ K V P+ S + S +G
Sbjct: 78 VRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAK-VVIPERGSVTFVSTIG 137
Query: 131 FTDWRKDL-----------------DSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVV 190
D R L +K + R L +MA+K++YE +++VV
Sbjct: 138 QLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYENAKVVENVV 197
Query: 191 NDHWKMKMLHYYAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADF 250
+ HWKM ++ + WND+Q + +TQ F+F + D+N+ VI+FRGT P DA DW D D+
Sbjct: 198 DLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDADDWGTDFDY 257
Query: 251 SWYEIEGIGRIHSGFMKALGL---QKATGWPKNLPPSPDNRQ-------------FAYYT 310
SWYE+ +G++H GF++A+GL T + NL + + AYY
Sbjct: 258 SWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDMVERSAYYA 317
Query: 311 LRQQLRDIAKANDKARFIITGHSLGGALATLFVTLLSFHQESTLLEKLQAVYTYGQPRVG 370
+R L+ + ++ ARF++TGHSLGGALA LF TLL ++E+ ++++L VYT+GQPR+G
Sbjct: 318 VRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFGQPRIG 377
Query: 371 DQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIPADGIVFEYKHFGGCVFFNSLYKGKILR 430
+++ FM +++ +Y+R VY ND+VPR+P D F YKHFG C+F++S Y
Sbjct: 378 NREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFYDSFYNETKAE 437
Query: 431 EQPNKNYFSLVWLIPKYLFAWWELIRSFIIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAH 464
++P+ N + L + I ++ A WEL+R + G Y EG R+ GL+IPG + H
Sbjct: 438 DEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYKEGWFRILFRLMGLVIPGLSDH 497
BLAST of Spg016466 vs. ExPASy Swiss-Prot
Match:
Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)
HSP 1 Score: 286.6 bits (732), Expect = 5.4e-76
Identity = 176/506 (34.78%), Postives = 263/506 (51.98%), Query Frame = 0
Query: 9 SKSYMILKPENANLLDLFLFILPFGFRK-RKFIDCPEGKEDSYRSFANRW--LIFISMLL 68
S S++I+ P+ LDLF I+ KF + + + ++ +RW +FI +L
Sbjct: 9 STSHLIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRWPVSVFICKIL 68
Query: 69 QLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYRSVVGF 128
QLF AA L L LL NF N G LG+L + + + P D+ S +G+
Sbjct: 69 QLF-QMPAAMLGHLTDFLL---NFYYQNHGFLGILRNIFLIRLKI-PKRGEADFISTIGY 128
Query: 129 TDWRKDL------------------DSSIKPDDTFRYYGA--------LTMMAAKVSYEF 188
D R DL + S+K + GA L +MA+K++YE
Sbjct: 129 LDSRMDLHGTPMVSHQADEVISNADNPSLKEGHNSKIKGALGNRSLMDLCIMASKLAYEN 188
Query: 189 LPFIQSVVNDHWKMKMLHYYAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAY 248
++ VV +HWKM + Y N FQ+ T AFIF + D+N+ VI+FRGT P
Sbjct: 189 TKVVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSIP 248
Query: 249 DWQVDADFSWYEIEGIGRIHSGFMKALGL------------------QKATGWPKNLPPS 308
+W D DFS + G +H GF++A+GL T K +
Sbjct: 249 NWCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEMA 308
Query: 309 PDNRQFA----YYTLRQQLRDIAKANDKARFIITGHSLGGALATLFVTLLSFHQESTLLE 368
PD+ +A Y+ L+ + K + A+F++TGHSLGGALA LF +L QE+ +L+
Sbjct: 309 PDHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETEVLD 368
Query: 369 KLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIPADGIVFEYKHFGG 428
+L VYT+GQPR+G+ FM N ++ +Y+R VY ND+VPR+P D + F ++HFG
Sbjct: 369 RLLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPFDDVFFTFEHFGT 428
Query: 429 CVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPIVKGSVYYEGLLMKG 464
C++++S + G +E+P++N F + I ++ AWWEL RSFI+ V G+ Y E +
Sbjct: 429 CIYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNHVYGAEYKETWESRM 488
BLAST of Spg016466 vs. ExPASy Swiss-Prot
Match:
O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)
HSP 1 Score: 69.3 bits (168), Expect = 1.4e-10
Identity = 58/197 (29.44%), Postives = 90/197 (45.69%), Query Frame = 0
Query: 181 FWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGI---GR 240
F F++ F + + + V++FRG+ ++ +W + +F EI I R
Sbjct: 73 FLYSFEDSGVGDVTGFLALDNTNKLIVLSFRGSRSIE--NWIGNLNFDLKEINDICSGCR 132
Query: 241 IHSGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGG 300
H GF + W + TLRQ++ D + + R + TGHSLGG
Sbjct: 133 GHDGF--------TSSW-----------RSVADTLRQKVEDAVREHPDYRVVFTGHSLGG 192
Query: 301 ALATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSN 360
ALAT+ L + V++YG PRVG++ FAEF+ + G YR ++N
Sbjct: 193 ALATVAGADLRGNGYDI------DVFSYGAPRVGNRAFAEFLT---VQTGGTLYRITHTN 237
Query: 361 DLVPRIPADGIVFEYKH 375
D+VPR+P F Y H
Sbjct: 253 DIVPRLPPR--EFGYSH 237
BLAST of Spg016466 vs. ExPASy Swiss-Prot
Match:
P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)
HSP 1 Score: 66.2 bits (160), Expect = 1.2e-09
Identity = 58/179 (32.40%), Postives = 80/179 (44.69%), Query Frame = 0
Query: 202 DSNITV-IAFRGTSPLDAYDWQVDADF---SWYEIEGIGRIHSGFMKALGLQKATGWPKN 261
DS T+ I FRG+S + +W D F S+ + G ++H GF+ + G
Sbjct: 164 DSEKTIYIVFRGSSSI--RNWIADLTFVPVSYPPVSGT-KVHKGFLDSYG---------- 223
Query: 262 LPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFVTLLSFHQESTLLE 321
N A + D K + +TGHSLGGA A L L + +E L
Sbjct: 224 ---EVQNELVA------TVLDQFKQYPSYKVAVTGHSLGGATA-LLCALDLYQREEGLSS 283
Query: 322 KLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIPADGIVFEYKHFG 377
+YT GQPRVGD FA ++V+T G Y R V D+VP +P F + H G
Sbjct: 284 SNLFLYTQGQPRVGDPAFANYVVST----GIPYRRTVNERDIVPHLPP--AAFGFLHAG 313
BLAST of Spg016466 vs. ExPASy TrEMBL
Match:
A0A1S3BUY0 (uncharacterized protein LOC103493549 OS=Cucumis melo OX=3656 GN=LOC103493549 PE=4 SV=1)
HSP 1 Score: 754.6 bits (1947), Expect = 2.6e-214
Identity = 351/483 (72.67%), Postives = 412/483 (85.30%), Query Frame = 0
Query: 1 MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
M+ E+EFSK+Y+ILKPENANLLDLFLF+LPFG RK+KFIDCP GKED + +FA+RWLIF
Sbjct: 1 MVLMENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFADRWLIF 60
Query: 61 ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
+SMLLQ L IA PLA LD+ LLK FNFISFNGG+LG+LSK+++ K FV+ E+S +Y
Sbjct: 61 VSMLLQFILALIATPLAKLDAFLLKLFNFISFNGGLLGILSKILRGKGFVKVKEDSSEYT 120
Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
SVVGF DWR+DLDSSIK + +FRYY ALT+MA K+SYE PF+QSVVND WKMK+L Y+
Sbjct: 121 SVVGFADWRRDLDSSIKSEKSFRYYSALTVMATKISYESKPFVQSVVNDRWKMKLLGYFN 180
Query: 181 FWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHS 240
FWNDFQ K TTQAF F+N A D N+ ++AFRGTSPLDAYDWQVD D SWYEIEG+GRIHS
Sbjct: 181 FWNDFQGKATTQAFTFENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHS 240
Query: 241 GFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALA 300
GFMKALGLQKATGWPK LP + +FAYYTLR+QLRDIAKAN+KARFI TGHSLGGALA
Sbjct: 241 GFMKALGLQKATGWPKELPKT-QTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALA 300
Query: 301 TLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLV 360
TLFVT+LS H EST+LEKL +VYTYGQPRVGDQQFA+FMVN + KYGFKY+RYVYS DLV
Sbjct: 301 TLFVTVLSLHDESTILEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLV 360
Query: 361 PRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFI 420
PR+P+D ++F+YKHFG CV+FNSLYKG+I++EQPN+NYFSL+W+IPKYL AWWELIRSFI
Sbjct: 361 PRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRSFI 420
Query: 421 IPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYV 480
IP VKG YYE LLMKG R+ GL+IPG +AHFP +YVN TRLG L +PD +EDP LG +
Sbjct: 421 IPFVKGFDYYESLLMKGARLVGLLIPGLSAHFPLNYVNVTRLGKLNVPDEVEDPFLGYEI 480
Query: 481 EAD 484
E D
Sbjct: 481 EDD 482
BLAST of Spg016466 vs. ExPASy TrEMBL
Match:
A0A0A0L2D5 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639870 PE=4 SV=1)
HSP 1 Score: 748.4 bits (1931), Expect = 1.9e-212
Identity = 350/483 (72.46%), Postives = 410/483 (84.89%), Query Frame = 0
Query: 1 MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
M+ E+EFSK+Y+ILKPENANLLDLFLF+LPFG RK+KFIDCP GKED + +F +RWLI
Sbjct: 1 MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60
Query: 61 ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
+SMLLQ L IA PLA LD+ LLK FNFISFNGG LG+LSK+++ K FV+ +E+S +Y
Sbjct: 61 VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120
Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
SVVGF DWR+DLDSSIKP+ +FRYY ALT MA K+SYE PF+QSVVND WKMK+L Y+
Sbjct: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180
Query: 181 FWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHS 240
FWNDFQ +TTTQAF+F+N A D N+T++AFRGTSPLDAYDWQVD DFSWYEIE +G IHS
Sbjct: 181 FWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHS 240
Query: 241 GFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALA 300
GFMKALGLQKATGWPK LP + +FAYYTLR+QLRDIAKAN+KARFI TGHSLGGALA
Sbjct: 241 GFMKALGLQKATGWPKELPKT-QTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALA 300
Query: 301 TLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLV 360
TLFVT+L H EST+LEKL +VYTYGQPRVGDQQFA+FM+N I KYGFKY+RYVYS DLV
Sbjct: 301 TLFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLV 360
Query: 361 PRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFI 420
PR+P+D ++F+YKHFG CV+FNSLYKG+I++EQPNKNYFSL+W+IPKYL AWWELIRSFI
Sbjct: 361 PRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFI 420
Query: 421 IPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYV 480
IP VKG YYE LLMKG R+ GL+IPG AHFP +YVN TRLG L +PD +EDPIL +
Sbjct: 421 IPFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEI 480
Query: 481 EAD 484
E D
Sbjct: 481 EDD 482
BLAST of Spg016466 vs. ExPASy TrEMBL
Match:
A0A6J1FNK8 (uncharacterized protein LOC111445886 OS=Cucurbita moschata OX=3662 GN=LOC111445886 PE=4 SV=1)
HSP 1 Score: 741.5 bits (1913), Expect = 2.3e-210
Identity = 353/484 (72.93%), Postives = 408/484 (84.30%), Query Frame = 0
Query: 1 MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
M+ KE EFSK+YMILKP +A LLDLFLF+LPFG RK KFIDCPEG EDSY +F +RW IF
Sbjct: 1 MVWKECEFSKNYMILKPNDAKLLDLFLFLLPFGLRKTKFIDCPEGNEDSYNNFTDRWSIF 60
Query: 61 ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
IS+LLQ+ L A A PLA LDS L+ FNFISFNGGILGLL+K++K K V+P+E+S DY
Sbjct: 61 ISVLLQIILAAAATPLAKLDSFLVNLFNFISFNGGILGLLAKILKGKGLVKPNEDSSDYT 120
Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
SVVGF DWR DLDSSI DTFRYYG LT+MAAK++YE PF+QSVVND WKMK+L ++
Sbjct: 121 SVVGFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFN 180
Query: 181 FWNDFQNKTTTQAFIFQNIATDS-NITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIH 240
FWNDFQ K TTQAF+FQN ATD NI VIAFRGTSPLD YDWQVDADFSWY+IEG+GRIH
Sbjct: 181 FWNDFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTYDWQVDADFSWYDIEGVGRIH 240
Query: 241 SGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGAL 300
SGFMKALGLQKATGWPK L + FAYYTLRQ+LRDIAKAN KARFIITGHSLGGAL
Sbjct: 241 SGFMKALGLQKATGWPKEL--TKPQHDFAYYTLRQKLRDIAKANAKARFIITGHSLGGAL 300
Query: 301 ATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDL 360
ATLFVTLLS H E+ +LEKLQ VYTYGQPRVGD++FAEFMVN++ +YGFKYYRYVYS+DL
Sbjct: 301 ATLFVTLLSLHDETPILEKLQGVYTYGQPRVGDRRFAEFMVNSVQQYGFKYYRYVYSSDL 360
Query: 361 VPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSF 420
VPRIP+DG++F+YKHFG ++FN+LYKG+I++ QPNKNYFSL+W+IPKYL AWWEL+RSF
Sbjct: 361 VPRIPSDGVIFKYKHFGRSIYFNTLYKGRIVKGQPNKNYFSLLWVIPKYLSAWWELVRSF 420
Query: 421 IIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNY 480
I P+V G YYE LLM R+ GLIIPG AAHFP +YVNSTRLG L+ PD +EDPIL +
Sbjct: 421 ITPLVWGFDYYESLLMSAARIIGLIIPGLAAHFPLNYVNSTRLGKLKAPDEVEDPILEDD 480
Query: 481 VEAD 484
+E D
Sbjct: 481 IEGD 482
BLAST of Spg016466 vs. ExPASy TrEMBL
Match:
A0A6J1CEI9 (uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010491 PE=4 SV=1)
HSP 1 Score: 735.3 bits (1897), Expect = 1.6e-208
Identity = 346/470 (73.62%), Postives = 406/470 (86.38%), Query Frame = 0
Query: 1 MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKR-KFIDCPEGKEDSY-RSFANRWL 60
M SKEHEFSK+Y+ILKP+NAN++DLFLF+LPFGFRKR KFIDCPEGKEDSY SF NRWL
Sbjct: 1 MASKEHEFSKNYLILKPQNANIVDLFLFLLPFGFRKRTKFIDCPEGKEDSYTSSFTNRWL 60
Query: 61 IFISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPD 120
IFIS++LQ+ L AIA PLA LD+ LL FNFISFNGGILGLLS+++K + V+PDENS +
Sbjct: 61 IFISIVLQIILLAIATPLAKLDAFLLNVFNFISFNGGILGLLSRILKGESLVKPDENSAE 120
Query: 121 YRSVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHY 180
Y SVVGF DWR+DLD SIK ++ FRYYG LT+MAA++SYE PF+ SVVND WKMK+L +
Sbjct: 121 YTSVVGFADWRRDLDKSIKAEEIFRYYGGLTVMAAQLSYESKPFVLSVVNDRWKMKLLGH 180
Query: 181 YAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRI 240
+ FWNDFQ K TTQAF+FQN A D N+TV+AFRGTSPLDAYDWQVDADFSWY+IEG+GRI
Sbjct: 181 FNFWNDFQGKATTQAFMFQNTAADPNVTVVAFRGTSPLDAYDWQVDADFSWYDIEGVGRI 240
Query: 241 HSGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGA 300
HSGFMKALGLQKATGWP+ L S FAYYTLRQ+LRDIAK+ND A+FIITGHSLGGA
Sbjct: 241 HSGFMKALGLQKATGWPEELVKS--KHHFAYYTLRQKLRDIAKSNDNAKFIITGHSLGGA 300
Query: 301 LATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSND 360
LA LFVT+LS H++S +LEKLQAVYTY QPRVGD++FAEFMVNT+ KYGFKY+RYVYS+D
Sbjct: 301 LAALFVTVLSLHRDSAVLEKLQAVYTYSQPRVGDRRFAEFMVNTVKKYGFKYHRYVYSSD 360
Query: 361 LVPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRS 420
LVPR+P DG++F+YKHFG C++FNSLYKG+I++E+PNKNYFSL+W++PKYL AWWELIRS
Sbjct: 361 LVPRVPFDGVIFKYKHFGRCIYFNSLYKGRIVKEEPNKNYFSLIWVMPKYLNAWWELIRS 420
Query: 421 FIIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIP 469
FI+P+VKG Y+E LLMKG RV GL IPG AAH P DYVNS RLG P
Sbjct: 421 FIVPLVKGFDYHESLLMKGARVVGLFIPGLAAHIPTDYVNSIRLGKFNPP 468
BLAST of Spg016466 vs. ExPASy TrEMBL
Match:
A0A6J1J3Y9 (uncharacterized protein LOC111481121 OS=Cucurbita maxima OX=3661 GN=LOC111481121 PE=4 SV=1)
HSP 1 Score: 734.2 bits (1894), Expect = 3.6e-208
Identity = 350/484 (72.31%), Postives = 409/484 (84.50%), Query Frame = 0
Query: 1 MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
M+SK+ EFSK+YMILKP +A LLDLFLF+LPFG RK KFIDCPEG E SY +F +RW IF
Sbjct: 1 MVSKKCEFSKNYMILKPNDAKLLDLFLFLLPFGLRKTKFIDCPEGNEASYSNFTDRWFIF 60
Query: 61 ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
IS+LLQ+ L A A PL+ LDS L+ FNFISFNGGILGLLSK++K K V+P+E+S DY
Sbjct: 61 ISILLQIILAAAATPLSKLDSFLVNLFNFISFNGGILGLLSKILKGKGLVKPNEDSSDYT 120
Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
SV+GF DWR DLDSSI DTFRYYG LT+MAAK++YE PF+QSVVND WKMK+L ++
Sbjct: 121 SVLGFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFN 180
Query: 181 FWNDFQNKTTTQAFIFQNIATDS-NITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIH 240
FWNDFQ K TTQAF+FQN ATD NI VIAFRGTSPLD DWQV+ADFSWY+IEG+GRIH
Sbjct: 181 FWNDFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTDDWQVNADFSWYDIEGLGRIH 240
Query: 241 SGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGAL 300
SGFMKALGLQKATGWPK L + FAYY+LRQ+LRDIAKAN KARFIITGHSLGGAL
Sbjct: 241 SGFMKALGLQKATGWPKEL--TKPQHDFAYYSLRQKLRDIAKANAKARFIITGHSLGGAL 300
Query: 301 ATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDL 360
ATLFVTLLS H E+T+LEKLQ VYTYGQPRVGD++FAEFMVNT+ KYGFKYYRYVYS+DL
Sbjct: 301 ATLFVTLLSLHDETTILEKLQGVYTYGQPRVGDRRFAEFMVNTVQKYGFKYYRYVYSSDL 360
Query: 361 VPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSF 420
VPRIP+DG++F+YKHFG ++FNSLYKG+I++EQPNKNYFSL+W+IPKYL AWWEL+RSF
Sbjct: 361 VPRIPSDGVIFKYKHFGRSIYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELVRSF 420
Query: 421 IIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNY 480
I P++ G +YE LLM R+ GLIIPG AAHFP +YVNS RLG L++PD IEDPIL +
Sbjct: 421 ITPLMWGFDHYESLLMSAARMVGLIIPGLAAHFPLNYVNSIRLGKLKVPDEIEDPILEDD 480
Query: 481 VEAD 484
+E +
Sbjct: 481 IEGE 482
BLAST of Spg016466 vs. TAIR 10
Match:
AT1G45201.1 (triacylglycerol lipase-like 1 )
HSP 1 Score: 431.4 bits (1108), Expect = 9.7e-121
Identity = 217/471 (46.07%), Postives = 301/471 (63.91%), Query Frame = 0
Query: 7 EFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSY-RSFANRWLIFISMLL 66
+F SY ++ P A+ LDL L + +FID P + RSFA+RW++ +++ L
Sbjct: 7 KFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFL 66
Query: 67 QLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYRSVVGF 126
Q L ++ P A + L N ++ NGG L+ L+ K V+PD++S Y S +G
Sbjct: 67 QKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGK-LVKPDKSSATYTSFIGC 126
Query: 127 TDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWNDF 186
+D R +LD I + Y L++MA+K+SYE P+I SVV + WKM ++ Y F+N F
Sbjct: 127 SDRRIELDEKINV-GSIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAF 186
Query: 187 QNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKA 246
Q TQAF+F+ +T+ ++ V++FRGT P +A DW D D SWYE++ +G++H+GF +A
Sbjct: 187 QESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRA 246
Query: 247 LGLQKATGWPK-NLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFV 306
LGLQK GWPK N+ Q+AYYT+RQ LRD N ++I+TGHSLGGALA LF
Sbjct: 247 LGLQK-DGWPKENISLL---HQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFP 306
Query: 307 TLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIP 366
+L+ H E LL+KL+ +YT+GQPRVGD+ F EFM + K+G +Y R+VY+ND+VPR+P
Sbjct: 307 AILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVP 366
Query: 367 ADG-IVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPI 426
D +F YKH+G C FNSLYKGK+ + PN NYF+L+WLIP+ L WE IRSFI+
Sbjct: 367 FDDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTGLWEFIRSFILQF 426
Query: 427 VKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDP 475
KG Y E LM+ VRV G++ PG + HFP DYVNSTRLG L P P
Sbjct: 427 WKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRPPPTTTP 471
BLAST of Spg016466 vs. TAIR 10
Match:
AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )
HSP 1 Score: 422.9 bits (1086), Expect = 3.5e-118
Identity = 206/465 (44.30%), Postives = 301/465 (64.73%), Query Frame = 0
Query: 9 SKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGK-EDSYRSFANRWLIFISMLLQL 68
+K+Y +L P A + DL + RKFI E + ED F RW+IF+S+++Q
Sbjct: 4 TKNYFVLDPREATVSDLIHLLFSSDLGDRKFIHSSEERIEDDLSRFRRRWIIFVSIVIQK 63
Query: 69 FLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYRSVVGFTD 128
+ PL + L N +S NGG L +L L K + P++ S + S+ G D
Sbjct: 64 LIILFKKPLYFVGFSLACWLNLLSSNGGFLMILPNLFKGN-IIWPEKASATFASLNGNLD 123
Query: 129 WRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWNDFQN 188
R +L+ ++ RY L++MA+K+SYE + F+ SV+++HWKM +L +Y+ WN +Q
Sbjct: 124 RRVELNPKVERGSK-RYKAMLSIMASKLSYENINFVSSVLHNHWKMDLLGFYSCWNGYQK 183
Query: 189 KTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKALG 248
+ +T+ + ++ +TD N+ +++FRGT P DA DW D D SWYE++ +G+IH GFMKALG
Sbjct: 184 QRSTEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKIHGGFMKALG 243
Query: 249 LQKATGWPK--NLPPSPD-NRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFV 308
LQK GWPK NL + + +AYYT+R+ L++I N ++FI+TGHSLGGALA LF
Sbjct: 244 LQK-EGWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTGHSLGGALAILFT 303
Query: 309 TLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIP 368
+L H E +LE+L+ VYT+GQPRVGD++F FM +++ K+ KY RYVY ND+VPR+P
Sbjct: 304 AVLVMHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDSLKKFDVKYERYVYCNDMVPRLP 363
Query: 369 ADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPIV 428
D +KHFG C++++S YKGK+ E+PNKNYF+LVW++PK + A WELIRSF++P
Sbjct: 364 FDDKTLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKIMNALWELIRSFVMPYW 423
Query: 429 KGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPD 470
KG + EG ++ RV L+IPG AHFP +Y+N T LG +PD
Sbjct: 424 KGGEFREGWFLRCFRVVALLIPGLPAHFPNEYINVTLLG--DLPD 463
BLAST of Spg016466 vs. TAIR 10
Match:
AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )
HSP 1 Score: 393.7 bits (1010), Expect = 2.2e-109
Identity = 206/480 (42.92%), Postives = 284/480 (59.17%), Query Frame = 0
Query: 1 MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
M S + + + Y+IL+PE +L + K + +D E +E S+R +RWLIF
Sbjct: 2 MNSDDDDEPRGYLILRPEELRPWELVRLLFSGDIEKPRSVDSSETEEHSFR---HRWLIF 61
Query: 61 ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYR 120
+S++L L + LA + S L NF+S N S L R V P S +Y+
Sbjct: 62 VSLVLLKLLRFFSKLLALVGSALEFSLNFLSNNS-----FSGLFLRGEVVMPQRTSENYQ 121
Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
S +G D R LD ++ +D +YY AL++MA+K++YE I+ VV +HW MK L
Sbjct: 122 SFIGHLDTRVSLDMTLNREDGEKYYAALSIMASKIAYENAARIKHVVENHWNMKYLGLVD 181
Query: 181 FWNDFQNKTTTQAFIFQNIAT------DSNITVIAFRGTSPLDAYDWQVDADFSWYEIEG 240
+WN++Q K TTQAFI T V+AFRGT ++ DW D D +W+E+
Sbjct: 182 YWNEYQEKETTQAFIMSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDFDITWFELPN 241
Query: 241 IGRIHSGFMKALGLQKATGWPKNLPPSPDNRQ-FAYYTLRQQLRDIAKANDKARFIITGH 300
IG IH GFMKALGLQ WPK +PD + AYY++R L+ + N +F++TGH
Sbjct: 242 IGNIHGGFMKALGLQNNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTKFVLTGH 301
Query: 301 SLGGALATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRY 360
SLGGALA LF +L H E+ LLE++Q VYTYGQPRVGD +F EFM + KY KYYR+
Sbjct: 302 SLGGALAILFTAVLVIHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYNIKYYRF 361
Query: 361 VYSNDLVPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWW 420
VY+ND+VPR+P D +KHFG C++++ Y+ K++REQ ++N+F L +I A
Sbjct: 362 VYNNDIVPRLPYDDKDLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIKMMWSAIL 421
Query: 421 ELIRSFIIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIED 474
E IRSF I KGS Y EG L+KG R G+I+PG + H PQDYVN+TRL L + V D
Sbjct: 422 EFIRSFTIVAEKGSEYSEGWLLKGGRALGIIVPGVSNHTPQDYVNATRLTLPCVFQVYRD 473
BLAST of Spg016466 vs. TAIR 10
Match:
AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )
HSP 1 Score: 387.9 bits (995), Expect = 1.2e-107
Identity = 200/468 (42.74%), Postives = 288/468 (61.54%), Query Frame = 0
Query: 10 KSYMILKPENANLLDLFLFILPFGFRKRKFIDCP-EGKEDSYRSFANRWLIFISMLLQLF 69
K+Y +L P A + DL + RKF+D E E+ F RW+IF+S+++Q
Sbjct: 5 KNYFVLDPREATVTDLMRLLFYSDLENRKFVDTSVENLENRLCEFRGRWIIFVSIVVQKL 64
Query: 70 LGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYRSVVGFTDW 129
L + PL+ L L N S NGG + LVK + F+ P++ S + S+ G D
Sbjct: 65 LIILRKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGR-FIWPEKTSATFASINGNLDQ 124
Query: 130 RKD--LDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWNDFQ 189
+ + L SIK D RY L++MA+K++YE FI+SV+ DHW+M +L +Y+ NDF
Sbjct: 125 KVELGLGRSIKIGDE-RYKPLLSIMASKLAYENEHFIRSVLQDHWQMDLLGFYSCPNDFD 184
Query: 190 NKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKAL 249
+T+ + ++ + N+ V++FRGT P +A DW D D SW+ + +G+IH GFMKAL
Sbjct: 185 QTRSTEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGKIHGGFMKAL 244
Query: 250 GLQKATGWPKNLPPSPDNR---QFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLF 309
GL K GW + + Q AYYT+ +QL+++ + N ++FI++GHSLGGALA LF
Sbjct: 245 GLPK-EGWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSLGGALAILF 304
Query: 310 VTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRI 369
+L H E +LE+L+ VYT+GQPRVGD+ F +M + + ++ KY RYVY ND+VPR+
Sbjct: 305 TAVLIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVYCNDMVPRL 364
Query: 370 PADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPI 429
P D +KHFGGC++ +S YKGK+ E+PNKNYF++ W+IPK + A WELIRSFII
Sbjct: 365 PFDDKTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKIINAVWELIRSFIISC 424
Query: 430 VKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLL--QIPD 470
+G Y EG L+ R+ L+IPG AHFP +YVN LG Q+PD
Sbjct: 425 WQGREYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLGNFPPQVPD 469
BLAST of Spg016466 vs. TAIR 10
Match:
AT1G45201.2 (triacylglycerol lipase-like 1 )
HSP 1 Score: 342.0 bits (876), Expect = 7.8e-94
Identity = 172/386 (44.56%), Postives = 247/386 (63.99%), Query Frame = 0
Query: 7 EFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSY-RSFANRWLIFISMLL 66
+F SY ++ P A+ LDL L + +FID P + RSFA+RW++ +++ L
Sbjct: 7 KFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFL 66
Query: 67 QLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKLVKRKPFVQPDENSPDYRSVVGF 126
Q L ++ P A + L N ++ NGG L+ L+ K V+PD++S Y S +G
Sbjct: 67 QKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGK-LVKPDKSSATYTSFIGC 126
Query: 127 TDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWNDF 186
+D R +LD I + Y L++MA+K+SYE P+I SVV + WKM ++ Y F+N F
Sbjct: 127 SDRRIELDEKINV-GSIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAF 186
Query: 187 QNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKA 246
Q TQAF+F+ +T+ ++ V++FRGT P +A DW D D SWYE++ +G++H+GF +A
Sbjct: 187 QESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRA 246
Query: 247 LGLQKATGWPK-NLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFV 306
LGLQK GWPK N+ Q+AYYT+RQ LRD N ++I+TGHSLGGALA LF
Sbjct: 247 LGLQK-DGWPKENISLL---HQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFP 306
Query: 307 TLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIP 366
+L+ H E LL+KL+ +YT+GQPRVGD+ F EFM + K+G +Y R+VY+ND+VPR+P
Sbjct: 307 AILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVP 366
Query: 367 ADG-IVFEYKHFGGCVFFNSLYKGKI 390
D +F YKH+G C FNSLYKGK+
Sbjct: 367 FDDKYLFSYKHYGPCNSFNSLYKGKV 386
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038899591.1 | 6.6e-220 | 75.99 | triacylglycerol lipase OBL1-like [Benincasa hispida] | [more] |
XP_008452563.1 | 5.4e-214 | 72.67 | PREDICTED: uncharacterized protein LOC103493549 [Cucumis melo] | [more] |
XP_011654113.1 | 3.9e-212 | 72.46 | uncharacterized protein LOC101203640 [Cucumis sativus] >KGN55199.1 hypothetical ... | [more] |
XP_022940183.1 | 4.7e-210 | 72.93 | uncharacterized protein LOC111445886 [Cucurbita moschata] | [more] |
XP_022139642.1 | 3.4e-208 | 73.62 | uncharacterized protein LOC111010491 [Momordica charantia] | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3ZP85 | 6.4e-85 | 37.16 | Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1 | [more] |
F4JFU8 | 1.6e-83 | 34.76 | Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1 | [more] |
Q5VKJ7 | 5.4e-76 | 34.78 | Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1 | [more] |
O59952 | 1.4e-10 | 29.44 | Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1 | [more] |
P19515 | 1.2e-09 | 32.40 | Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2 | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3BUY0 | 2.6e-214 | 72.67 | uncharacterized protein LOC103493549 OS=Cucumis melo OX=3656 GN=LOC103493549 PE=... | [more] |
A0A0A0L2D5 | 1.9e-212 | 72.46 | Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639870 PE... | [more] |
A0A6J1FNK8 | 2.3e-210 | 72.93 | uncharacterized protein LOC111445886 OS=Cucurbita moschata OX=3662 GN=LOC1114458... | [more] |
A0A6J1CEI9 | 1.6e-208 | 73.62 | uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010... | [more] |
A0A6J1J3Y9 | 3.6e-208 | 72.31 | uncharacterized protein LOC111481121 OS=Cucurbita maxima OX=3661 GN=LOC111481121... | [more] |